CN109164100B - Test strip for rapidly detecting pesticide - Google Patents

Test strip for rapidly detecting pesticide Download PDF

Info

Publication number
CN109164100B
CN109164100B CN201811274435.XA CN201811274435A CN109164100B CN 109164100 B CN109164100 B CN 109164100B CN 201811274435 A CN201811274435 A CN 201811274435A CN 109164100 B CN109164100 B CN 109164100B
Authority
CN
China
Prior art keywords
test strip
filter membrane
pesticide
mmol
strip
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201811274435.XA
Other languages
Chinese (zh)
Other versions
CN109164100A (en
Inventor
韩磊
吴加慧
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Agricultural University
Original Assignee
Qingdao Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Agricultural University filed Critical Qingdao Agricultural University
Priority to CN201811274435.XA priority Critical patent/CN109164100B/en
Publication of CN109164100A publication Critical patent/CN109164100A/en
Application granted granted Critical
Publication of CN109164100B publication Critical patent/CN109164100B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The invention relates to the fields of nano catalysis, environmental protection and analytical chemistry, and particularly relates to a test strip for rapidly detecting pesticides. Ferroferric oxide nano particles with peroxidase-imitated activity are generated on a test strip in situ, acetylcholinesterase and choline oxidase are modified, when an organophosphorus pesticide exists, the activity of the acetylcholinesterase can be irreversibly inhibited, the amount of choline generated by hydrolysis of acetylcholine is reduced, hydrogen peroxide generated by cascade reaction is reduced, and the color development of a chromogenic substrate is lightened. Furthermore, interference of other substances in the sample can be eliminated by the chromatography phenomenon of the test paper. Convenient quantitative detection of acetylcholine can be realized by mobile phone photographing and mobile phone software analysis. The invention can be used in the fields of pesticide residue analysis, biological medicine, environmental monitoring and the like.

Description

Test strip for rapidly detecting pesticide
Technical Field
The invention relates to the field of analytical chemistry, and particularly relates to a test strip for rapidly detecting pesticides.
Technical Field
Organophosphorus Pesticides (OP) are the most widely used agricultural pesticides and play an important role in improving agricultural productivity and preventing diseases and pests. However, organophosphorus pesticides can inhibit the activity of acetylcholinesterase in human body, thus causing various diseases of human body, such as senile dementia, Parkinson's disease, apoplexy and the like. Meanwhile, due to the high residue characteristic of the organophosphorus pesticide and the serious overuse or nonstandard operation in agricultural production, the high content residue of the pesticide on agricultural products is caused, and the ecological environment balance is seriously threatened. Therefore, the construction of a high-sensitivity detection platform and the realization of simple, convenient, rapid and high-sensitivity detection of the pesticide have important practical application values.
To date, many methods have been successfully applied to the sensing detection of organophosphorus pesticides, such as high performance liquid chromatography, gas chromatography-mass spectrometry, electrochemical analysis and the like. However, these methods tend to have long sample pretreatment times, typically 3-4 days, require special professionals, and rely on expensive large-scale instruments, which severely limits the large-scale application of the above methods in the field of pesticide residue detection.
The nano enzyme is a novel mimic enzyme, and becomes a human body due to excellent catalytic activity and potential application value in the aspects of biosensing, food technology, environmental protection and the likeThe focus of attention. The nano enzyme overcomes many defects of natural enzyme, such as high price, easy inactivation, strict requirement on storage condition and the like, and has great influence on the fields of biosensing, immunoassay, cancer diagnosis, treatment and the like. Currently, ferroferric oxide nanoenzyme has been reported to have peroxidase-like activity and can catalyze and oxidize hydrogen peroxide (H)2O2) The organic chromogenic substrate is oxidized to produce a color change. At present, reports on the in situ generation of nanoenzymes on paper are rare.
Compared with other detection modes, the test strip has the advantages of small volume, convenience in storage, simplicity in test, convenience in carrying and the like, and is popular with people. At present, some urine glucose test paper, HIV test paper and drug test paper are reported, but reports about test paper for in-situ generation of nano-enzyme and combination of nano-enzyme and smart phone for pesticide detection are rare.
Disclosure of Invention
The invention aims to provide a test strip for rapidly detecting pesticides.
In order to achieve the purpose, the invention adopts the technical scheme that:
the test strip can carry out qualitative and quantitative analysis on the pesticide.
Preferably, the test strip for rapidly detecting the pesticide has the following preparation process:
(1) cutting the filter membrane into filter membrane strips with the size of 0.2 multiplied by 2.5 cm by using a paper cutter;
(2) fixing two ends of the filter membrane strip by using a clamp, and reserving a gap of 2 mm at the position of 1-2 cm below the filter membrane strip;
(3) dripping equal-volume mixed liquid of 10% Bovine Serum Albumin (BSA) solution, 1 mmol/L hydroxysuccinimide (NHS) and 0.5 mmol/L1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC) into the filter membrane gap, incubating at room temperature for 30 min, washing with water and drying;
(4) adding 4 mmol/L ferric chloride solution (FeCl)3)2 mmol/L ferrous chloride solution (FeCl)2) And 16 mmol/L ammonia waterSequentially and uniformly dispersing the particles into the filter membrane gaps obtained in the previous step, incubating for 1 h at room temperature, washing with water and drying, wherein the filter membrane gaps are detection strips;
(5) and respectively dropwise adding acetylcholinesterase and choline oxidase with certain concentrations to the filter membrane gaps, incubating for 1 h, washing with water, and drying to obtain the test strip for detecting the pesticide.
It is further preferable that the test paper may be a nylon membrane, a nitrocellulose membrane, or a polytetrafluoroethylene membrane.
Further preferably, the use process is as follows:
(1) uniformly dispersing a pesticide sample, acetylcholine and an organic color-developing agent (2, 2' -diaza-bis (3-ethylbenzthiazoline-6-sulfonic acid) diamine salt (ABTS) or 3, 3', 5, 5' -Tetramethylbenzidine (TMB)) in a solution, immersing the lower edge of a test strip (the distance between the liquid surface and the lower edge of the test strip is less than 1 cm) into the mixed solution to serve as an experimental group, and immersing the lower edge of the test strip into a solution containing only the acetylcholine and the organic color-developing agent but not containing the pesticide sample to serve as a control group;
(2) after 2-5 minutes, taking out the test strip, observing the color of the test strip, and if the color of the experimental group is lighter than that of the control group, determining that the pesticide is contained;
(3) under the irradiation of the LED lamp, a smart phone is used for photographing, the RGB value on the detection test strip is read by the color recognizer, and the specific numerical value is substituted into the standard curve equation to carry out quantitative detection on the pesticide.
Still more preferably, the pesticide may be an organophosphorus pesticide or a carbamate pesticide.
The invention has the following effects:
1. the qualitative and quantitative test strip capable of rapidly detecting the pesticide on site is prepared;
2. the invention generates Fe in situ on paper3O4The preparation steps of the test strip are simplified by imitating enzyme materials;
3. the invention utilizes the in-situ generation of Fe3O4BSA on the enzyme-like material realizes the rapid speed of two biological enzymesThe stability of the natural enzyme is improved by fixing, and the active pH range of the natural enzyme can be changed, so that the two biological enzymes and the enzyme imitation material can simultaneously exert the activity;
4. the test strip prepared by the invention can eliminate the interference of the pigment and other impurities in a real sample through a chromatography strategy.
Drawings
FIG. 1 is a schematic diagram of a test strip for detecting pesticides provided by an embodiment of the present invention;
FIG. 2 is a diagram showing the effect of the test strip on the inhibition of active pesticides provided by the embodiment of the invention;
FIG. 3 is a photograph of a test strip provided in an embodiment of the present invention;
fig. 4 is a working curve of pesticide detection provided by the embodiment of the invention.
Detailed Description
In order to more clearly and more deeply illustrate the contents of the present invention, some examples will be further illustrated below, but the present invention is not limited to the illustrated examples. The specific experimental conditions or methods in the following examples, if not noted, were carried out according to conventional conditions or methods in the art.
Example 1
Preparation of pesticide detection test strip
(1) Cutting the filter membrane into filter membrane strips with the size of 0.2 multiplied by 2.5 cm by using a paper cutter;
(2) fixing two ends of the filter membrane strip by using a clamp, and reserving a gap of 2 mm at a position of 1-2 cm below the filter membrane strip for preparing a subsequent test strip;
(3) dropwise adding a mixed liquid of 10% Bovine Serum Albumin (BSA) solution, 1 mmol/L NHS and 0.5 mmol/L EDC in the same volume into the gap of the test strip (BSA is used for improving the adsorption capacity of a nylon filter membrane and is used as a template for the next reaction, EDC/NHS is used for activating carboxyl on BSA), incubating for 30 min at room temperature, washing with water and drying;
(4) 4 mmol/L FeCl32 mmol/L FeCl2And 16 mmol/L ammonia water are sequentially and uniformly dispersed into the filter membrane gaps obtained in the previous step in equal volume for in-situ generation of the four components with BSA as a templateFerroferric oxide enzyme-like material (Fe)3O4@ BSA), incubation for 1 h at room temperature, washing with water and drying;
(5) respectively dripping AchE and ChOx with certain concentration on the filter membrane gap to obtain Fe3O4And (3) connecting carboxyl on the @ BSA and amino on the enzyme through an amide reaction, washing away the unconnected enzyme with water, then drying at room temperature, and removing the clamp to obtain the pesticide detection test strip.
Example 2
The detection area in the test strip has the activities of AchE, ChOx and pseudoperoxidase under the weak acid condition, the AchE can catalyze the Ach to generate choline, and the ChOx can catalyze the choline to generate H2O2Peroxidase-like enzymes catalyze H2O2And an organic color developing agent to perform color development reaction, and when the pesticide exists, the activity of the AchE is irreversibly inhibited, so that H generated by cascade reaction is generated2O2The color of the organic color developing agent is reduced, and the detection schematic diagram is shown in the attached figure 1 of the specification.
And (3) verifying the active pesticide inhibition effect of the test strip:
experiment system a: the catalytic reaction system comprises a sample solution (0.1 mmol/L) containing Ach, fragments of the test strip obtained in example 1, an organic color-developing agent TMB (0.5 mmol/L) and an acetate buffer solution (pH 4.0, 100 mmol/L). After the detection strip is immersed in a buffer solution containing Ach and TMB at room temperature (25 ℃) for reaction for 5 min, the detection test strip is removed by centrifugation, and the absorbance value of the supernatant within 500-800 nm is detected by using an ultraviolet spectrophotometer.
Three additional experimental systems were performed: the reaction system b is a catalytic reaction system a, a detection test strip is not added, and the reaction is carried out for 5 minutes under the same condition as the experimental system, and then the light absorption value is detected; the catalytic reaction system c is only used for detecting the light absorption value after the test strip is immersed into acetate buffer solution (pH 4.0, 100 mmol/L) and is kept stand for 5 minutes under the same condition as the experimental system; in the reaction system d, after 30. mu. mol/L of OP is added into the experiment system a, the reaction is carried out for 5 minutes under the same other experiment conditions as the experiment system, and then the light absorption value is detected.
As shown in the accompanying figure 2 of the specification, the experimental system a shows an obvious peak near 650 nm, which indicates that the detection test strip has obvious activity of three enzymes at pH 4.0; the test system b has no obvious peak, which shows that if no detection strip is used as a catalyst, no obvious reaction is caused; the test system c has no obvious peak near 650 nm, which indicates that the peak of the test system a is not the response peak of the test strip; the peak shown by the experimental system d is lower than the curve a, which shows that the activity of the test strip is inhibited after the test strip is added with OP, and the feasibility of the experimental scheme is proved.
Example 3
Qualitative analysis of OP
The specific system is as follows: two previously prepared strips were prepared, one of which was immersed in a weakly acidic solution containing Ach (1 mM) and TMB (0.5 mmol/L) at the lower edge and the other in a weakly acidic solution containing OP (50 pmol/L), Ach (1 mmol/L) and TMB (0.5 mmol/L), and after 2-5 min, the color of both test strips was observed. As shown in the attached figure 3 of the specification, the test strip without OP has darker color than the test strip with OP, and the test strip prepared by the scheme can be used for qualitatively detecting OP.
Example 4
Quantitative detection of OP
The detection system comprises: the method comprises the steps of preparing acetylcholine (1 mM), organophosphorus pesticides (0-500 pM) with different concentrations and TMB (0.5 mM), immersing the prepared test strips into solutions containing OP with different concentrations respectively, sequencing the test strips according to the sequence of detection concentrations from small to large, taking a picture with a smart phone under the irradiation of an LED lamp after 2 min, reading specific RGB values by using a color recognizer, and calculating a standard working curve of the test strips according to the RGB values. Treating and taking BNIs a final parameter, and BNSpecific working curve is shown in FIG. 4, the linear range is 0-300 pM, and the equation is that y = -0.0027x +0.89 (R + G + B)2= 0.9983), indicating that the prepared test strip can quantitatively detect OP.

Claims (3)

1. A preparation method of a test strip for rapidly detecting pesticides is characterized by comprising the following steps:
(1) cutting the filter membrane into filter membrane strips with the size of 0.2 multiplied by 2.5 cm by a paper cutter;
(2) fixing two ends of the filter membrane strip by using a clamp, and reserving a gap of 2 mm at the position of 1-2 cm below the filter membrane strip; (3) dripping equal-volume mixed liquid of 10% Bovine Serum Albumin (BSA) solution, 1 mmol/L hydroxysuccinimide (NHS) and 0.5 mmol/L1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC) into the filter membrane gap, incubating at room temperature for 30 min, washing with water and drying;
(4) a4 mmo1/L solution of ferric chloride (FeCl) was added3)2 mmol/L ferrous chloride solution (FeC 1)2) And 16 mmol/L ammonia water are sequentially and uniformly dispersed into the filter membrane gaps obtained in the previous step in equal volume, and after L h incubation at room temperature, the filter membrane gaps are washed and dried, and the filter membrane gaps are detection strips;
(5) and respectively dripping the solutions of the acetylcholinesterase and the choline oxidase on the gaps of the filter membrane, incubating l h, washing with water and drying to obtain the test strip for detecting the pesticide.
2. The preparation method of the test strip for rapidly detecting the pesticide according to claim l, which is characterized in that: the filter membrane is one of a nylon membrane, a nitrocellulose membrane and a polytetrafluoroethylene membrane.
3. The application of the test strip for rapidly detecting the pesticide, which is prepared by the preparation method according to claim 1, is characterized in that the application process is as follows:
(1) immersing the lower edge of the test strip into a solution containing a pesticide sample, acetylcholine and an organic color developing agent to serve as an experimental group; immersing the lower edge of the test strip into a solution which only contains acetylcholine and an organic color developing agent and does not contain a pesticide sample to serve as a control group; the organic color developing agent is 2, 2' -dinitro-bis (3-ethylbenzthiazoline-6-sulfonic acid) diamine salt or 3, 3', 5, 5' -tetramethyl benzidine;
(2) after 2-5 minutes, taking out the test strip, observing the color of the test strip, and if the color of the experimental group is lighter than that of the control group, determining that the pesticide is contained;
(3) under the irradiation of the LED lamp, a smart phone is used for photographing, the RGB value on the detection test strip is read by the color recognizer, and the specific numerical value is substituted into the standard curve equation to carry out quantitative detection on the pesticide.
CN201811274435.XA 2018-10-30 2018-10-30 Test strip for rapidly detecting pesticide Active CN109164100B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811274435.XA CN109164100B (en) 2018-10-30 2018-10-30 Test strip for rapidly detecting pesticide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811274435.XA CN109164100B (en) 2018-10-30 2018-10-30 Test strip for rapidly detecting pesticide

Publications (2)

Publication Number Publication Date
CN109164100A CN109164100A (en) 2019-01-08
CN109164100B true CN109164100B (en) 2021-06-04

Family

ID=64876397

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811274435.XA Active CN109164100B (en) 2018-10-30 2018-10-30 Test strip for rapidly detecting pesticide

Country Status (1)

Country Link
CN (1) CN109164100B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111003859A (en) * 2019-11-23 2020-04-14 江苏久吾高科技股份有限公司 Zero-discharge treatment method and device for reclaimed water
CN113406068B (en) * 2021-07-08 2024-07-23 云南伦扬科技有限公司 Method for rapidly detecting glyphosate based on smart phone
CN113791064B (en) * 2021-09-08 2024-03-12 武汉谱信环保科技有限公司 Rapid detection method for quinfos pesticide residue
CN114384064B (en) * 2021-12-08 2023-11-10 江苏大学 Method for high-sensitivity and rapid detection of pesticide residues based on imprinted MOFs (metal-organic frameworks) probes
CN116380886B (en) * 2023-06-05 2023-08-15 中国农业大学 Method for intelligently detecting organophosphorus pesticide by double signals

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101387606A (en) * 2008-08-01 2009-03-18 中国科学院长春应用化学研究所 Method for detecting hydrogen peroxide or glucose based on enzyme simulation by ferroferric oxide magnetic nanometer particle
CN102399850A (en) * 2010-09-16 2012-04-04 上海市农业科学院 Rapid detection card for detecting pesticide residues, preparation method and application thereof
CN103712983A (en) * 2013-12-23 2014-04-09 江苏大学 Magnetic graphene enzyme-mimicking property-based acetylcholine visual-detection method
WO2018013697A2 (en) * 2016-07-12 2018-01-18 Purdue Research Foundation Devices systems, and methods for the detection of a target analyte using magnetic focus lateral flow immunoassay techniques
CN107607527A (en) * 2017-08-15 2018-01-19 江苏大学 A kind of Fast Determination of Pesticide Residue method based on nanogold analogue enztme
CN108084375A (en) * 2017-12-25 2018-05-29 中国农业科学院农业质量标准与检测技术研究所 A kind of triazole pesticide molecular blotting magnetic microsphere and its application
CN108387545A (en) * 2018-02-01 2018-08-10 上海工程技术大学 Ferroso-ferric oxide base composite carbon nanometer tube analogue enztme and its preparation method and application

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015130835A1 (en) * 2014-02-26 2015-09-03 The Trustees Of Princeton University Polymer nanoparticles

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101387606A (en) * 2008-08-01 2009-03-18 中国科学院长春应用化学研究所 Method for detecting hydrogen peroxide or glucose based on enzyme simulation by ferroferric oxide magnetic nanometer particle
CN102399850A (en) * 2010-09-16 2012-04-04 上海市农业科学院 Rapid detection card for detecting pesticide residues, preparation method and application thereof
CN103712983A (en) * 2013-12-23 2014-04-09 江苏大学 Magnetic graphene enzyme-mimicking property-based acetylcholine visual-detection method
WO2018013697A2 (en) * 2016-07-12 2018-01-18 Purdue Research Foundation Devices systems, and methods for the detection of a target analyte using magnetic focus lateral flow immunoassay techniques
CN107607527A (en) * 2017-08-15 2018-01-19 江苏大学 A kind of Fast Determination of Pesticide Residue method based on nanogold analogue enztme
CN108084375A (en) * 2017-12-25 2018-05-29 中国农业科学院农业质量标准与检测技术研究所 A kind of triazole pesticide molecular blotting magnetic microsphere and its application
CN108387545A (en) * 2018-02-01 2018-08-10 上海工程技术大学 Ferroso-ferric oxide base composite carbon nanometer tube analogue enztme and its preparation method and application

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Aptamer-controlled reversible inhibition of gold nanozyme activity for pesticide sensing;Weerathunge;《Analytical Chemistry》;20141231;第86卷(第24期);第11937-11941页 *
基于DNA的仿生酶的构建及检测应用;杨金金;《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》;20180315(第3期);B014-599 *

Also Published As

Publication number Publication date
CN109164100A (en) 2019-01-08

Similar Documents

Publication Publication Date Title
CN109164100B (en) Test strip for rapidly detecting pesticide
Hu et al. A label-free electrochemical immunosensor based on gold nanoparticles for detection of paraoxon
Eggins Biosensors: an introduction
Badawy et al. Bioactive paper sensor based on the acetylcholinesterase for the rapid detection of organophosphate and carbamate pesticides
Ciucu et al. Detection of pesticides using an amperometric biosensor based on ferophthalocyanine chemically modified carbon paste electrode and immobilized bienzymatic system
Zhang et al. Improved ELISA for tumor marker detection using electro-readout-mode based on label triggered degradation of methylene blue
Akyilmaz et al. Voltammetric determination of epinephrine by White rot fungi (Phanerochaete chrysosporium ME446) cells based microbial biosensor
Rawal et al. A comprehensive review of bilirubin determination methods with special emphasis on biosensors
Chang et al. Determination of glutamate pyruvate transaminase activity in clinical specimens using a biosensor composed of immobilized L-glutamate oxidase in a photo-crosslinkable polymer membrane on a palladium-deposited screen-printed carbon electrode
Nedeljko et al. Hybrid sol-gel based sensor layers for optical determination of biogenic amines
Huang et al. Recent developments on nanomaterial probes for detection of pesticide residues: A review
JP2007333714A (en) Electrochemical biosensor for measuring ultratrace amount of histamine
CN114414514B (en) Preparation method of manganese Prussian blue nano enzyme and application of manganese Prussian blue nano enzyme in alcohol concentration detection
Noguer et al. Biosensors based on enzyme inhibition: Detection of organophosphorus and carbamate insecticides and dithiocarbamate fungicides
Rastegarzadeh et al. An optical sensor for zinc determination based on Zincon as sensing reagent
Pundir et al. A comprehensive review of methods for determination of L-lysine with detailed description of biosensors
Cunha-Silva et al. Inhibited enzymatic reaction of crosslinked lactate oxidase through a pH-dependent mechanism
CN109253999B (en) Preparation and application of pesticide quick test card
CN111239124A (en) Hydrogen peroxide colorimetric detection method by utilizing platinum disulfide
Yang et al. Portable intelligent paper-based sensors for rapid colorimetric and smartphone-assisted analysis of hydrogen peroxide for food, environmental and medical detection applications
CN110227483A (en) A kind of novel magnetic nanometer simulation oxidizing ferment and its preparation method and application
CN109164098B (en) Acetylcholine detection test strip and application thereof
CN115479929A (en) Fluorescent test paper for detecting glyphosate, preparation method and application thereof
CN114002213A (en) Application of Cu/Au/Pt-MOFs and visual test paper thereof in detection of H2O2, Cys or glucose
US20030232322A1 (en) Nitrate sensor

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB03 Change of inventor or designer information
CB03 Change of inventor or designer information

Inventor after: Han Lei

Inventor after: Wu Jiahui

Inventor before: Li Feng

Inventor before: Wu Jiahui

Inventor before: Han Lei

GR01 Patent grant
GR01 Patent grant