CN109161502A - A kind of Preparation method and use of oil removing microbial inoculum, its composite dry powder microbial inoculum - Google Patents
A kind of Preparation method and use of oil removing microbial inoculum, its composite dry powder microbial inoculum Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C02F2101/00—Nature of the contaminant
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- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
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Abstract
The invention discloses the Preparation method and use of a kind of oil removing microbial inoculum, its composite dry powder microbial inoculum in contained Mud Treatment Technology field, which contains the mixed liquor of two kinds of bacterium solutions of GWN-1 bacterial strain, GWN-2 bacterial strain, and the viable count of every kind of bacterium solution is 109~1011A/ml；Two kinds of bacterium solutions are respectively GWN-1 bacterium solution: GWN-2 bacterium solution=1:(1.9~2.1 by volume) it is mixed；Its bacterium solution is fermented respectively, bacterium solution after two kinds of fermentations, then filters pressing and dry acquisition dry powder, is used in the processing of Sludge in Oilfields.The composite dry powder microbial inoculum is suitable for the direct biodegrade of High water cut, high oil-containing, high salinity Sludge in Oilfields, and environmental resistance is good, and preparation method has feature easy to operate, low in cost, is suitble to large-scale production application.
Description
Technical field
The present invention relates to the preparation and its application method of a kind of deoiling of sludge microbial inoculum, be particularly suitable for crude oil exploitation with
The oil patch of the oily sludge, drilling process generation that generate in process field, landing oil processing, belong at Sludge in Oilfields
Manage technical field.
Background technique
In the production of China's oil chemical industry, a large amount of oily sludge is generated every year, and yield is also rising year by year, oil-containing
Sludge is as the polluter in " National Hazard waste register ", it is necessary to it is handled, it otherwise can be to surrounding enviroment and production
Region generates different degrees of influence, and is also a kind of waste to resource, efficiently separates oily ingredient in recycling sludge, both
It is also to carry out the premise of oily sludge harmless treatment and resource utilization that the wasting of resources, which can be reduced,.
In recent years, researchers at home and abroad have carried out a large amount of research work to deoiling of sludge technology, form many skills
Art method.In deoiling of sludge process field, quenched isolation technics, incineration technology, solvent extraction oil recovery are mainly used both at home and abroad
The methods of technology, pyrogenic process modification treatment technology, but from application effect, more common problem is: at incineration method
It manages cost and operating cost height, treatment process generates SO2, the secondary pollutions such as CO and dust；Solvent method long flow path, complex process,
Solvent price is high, processing cost is high；Pyrogenic process processing investment height, complex process.
Relative to the physical treatment and chemical treatment method means of deoiling of sludge, sludge organism degreasing technique method is because of its tool
Have that processing cost is low, not will cause soil secondary pollution or leads to pollutant transfer, the features such as treatment process is simple, can effectively change
Kind sludge organism activity, by biochemical treatment, pollutant residual quantity can be greatly lowered, the research carried out in recent years compared with
It is more.For biological treatment, it is generally used adds efficient degrading bacterial strain (group) to reach effect of deoiling at this stage, or directly throw
The nutriments such as nitrogen phosphorus are added to carry out biostimulation to stimulate microorganism to grow, or by selecting plant and microorganism appropriate to assist
The same quick reparation to realize contaminated soil.But from research with from the point of view of applicable cases, biologic treating technique is generally existing following difficult
Select: the selection of Biochemical method sludge high-efficiency strain is difficult, microbial inoculum handles greasy filth excessive cycle；High oily sludge is difficult to adapt to,
Bacterial strain can not survive；Oily ingredient in sludge is mainly the heavy and super heavy crude of colloid Yu asphalitine ingredient, it is difficult to quilt
Microorganism is using degradation, therefore bioanalysis deoiling of sludge efficiency is generally lower, and the technology of Biochemical method oily sludge is not yet thrown
Enter scale application；Retrieval, which has no, is provided simultaneously with quick and efficient biological sludge processing method or bacterial strain.Research and establishment is efficient
Flora, optimal control condition shorten the main contents that process cycle is current this method research.
Existing patented technology analysis: in the patented technology of oil field sludge bioremediation, at oil field sludge biology
Reason microbial inoculum and method have related patents technology, and comparison of classification is analyzed as follows, such as a kind of biological air floatation method for oil-containing sludge
(ZL200510020666.4), add water is mixed to adjust sludge water pump agitation cycle, realize water-oil separating, mix and adjust aqueous average 95%, power
Consume it is excessive, and react after be mixed into microbial inoculum after 1 month compost oil content reach 0.3% standard；It is a kind of to utilize microbial degradation well site
The method (ZL201010550531.X) of oily sludge, live sludge are degraded to 1.1% by 10.55% through 56 days processing oil content,
Still it is unable to reach 0.3% standard；Oil degradation bacteria and its (ZL201410072228.1) is applied in a kind of oily sludge, in temperature
37 degree, salinity 0.5-5% isolate total petroleum hydrocarbon degradation rate 39.69% in bacterial strain 30d, practical oil field sludge salinity and processing temperature
Degree is generally more difficult to reach this requirement；By the oil degradation agent (ZL20110343690.5) in oil contaminated soil, process object
Do not include the oily sludge that generates in the oil-water system of oil field production link, is only limitted to the soil that earth's surface receives crude oil pollution, and its
Treatment effect is that 100g/kg degrades to 15g/kg, not up to 0.3% standard, and is not suitable for processed in oil field production link grease
The technical field of the High water cut disposal of oily sludge generated in journey.
Problems of the prior art are as follows:
1, the exploitation of efficient high activity oil-removing bacteria, it is desirable that be suitable for the condition of High water cut, high oily sludge.
2, it is preferably suitable for the industrial cheap substrate of efficient high activity oil-removing bacteria, enhances microorganism to salinity and other lifes
The tolerance level of object restraining factors effectively improves the activity of microorganism.
3, biodegradable treatment effeciency how is improved, process cycle is shortened.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the existing defects, a kind of oil removing microbial inoculum is provided, in high oily sludge
Under the conditions of efficient high activity oil removing, the present invention discloses the preparation method of the composite dry powder microbial inoculum of the oil removing microbial inoculum and
Purposes.
To solve the above-mentioned problems, a kind of oil removing microbial inoculum provided by the invention, for two containing GWN-1 bacterial strain, GWN-2 bacterial strain
The mixed liquor of kind bacterium solution, the viable count of every kind of bacterium solution are 109~1011A/ml；Two kinds of bacterium solutions are respectively GWN-1 bacterium by volume
Liquid: GWN-2 bacterium solution=1:(1.9~2.1) it is mixed；
Wherein,
GWN-1: Sphingobacterium (Latin name is Sphingobacterium sp.) is preserved in Chinese microorganism strain guarantor
It hides administration committee's common micro-organisms collection (CGMCC), preservation date: on March 20th, 2017, deposit number are as follows: CGMCC
No. 13902；
GWN-2: class Brevibacillus brevis (Latin name is Brevibacillus parabrevis) is preserved in the micro- life of China
Object culture presevation administration committee common micro-organisms collection (CGMCC), preservation date: on March 20th, 2017, deposit number
Are as follows: CGMCC No. 13903.
Above-mentioned GWN-1 bacterial strain, GWN-2 bacterial strain in the multiple oily wastewater ponds of Jiangsu oilfield, sludge drying bed by acquiring
Oily wastewater and mud sample are obtained again through screening after cultivating in the medium, are removed to Sludge in Oilfields with high activity
Oil effect.
To obtain dry bacteria, the invention also discloses the preparation method of the composite dry powder microbial inoculum of above-mentioned oil removing microbial inoculum, packets
Include following steps:
1) preparation of culture solution: being added peptone, NaCl in water, and the peptone additional amount is 9~11g/L, and NaCl's adds
Entering amount is 9~11g/L, then sterilizes 25~35 minutes, is cooled to room temperature at 118~125 DEG C；
2) it is inoculated with: two kinds of bacterium solutions in claim 1 being seeded to culture solution respectively and are fermented, inoculum concentration are as follows: 0.01v/v；
Fermentation temperature: 28~32 DEG C；Fermentation time: 40~56 h；Air is passed through in fermentation process into culture solution, is passed through sky per minute
The gas-liquid volume ratio of gas is 1:(0.8~3)；
3) filters pressing: after fermentation, by the fermentation liquid GWN-1 bacterium solution of two kinds of bacterium: GWN-2 bacterium solution according to volume ratio=1:(1.9~
2.1) mixed, then pressed dry with filter press, be allowed to moisture content weight content be 40~50%, filters pressing pressure be 0.15~
0.25MPa；
4) drying and crushing: remaining moisture content part is removed by drying tower, makes moisture content weight content 8~10%, drying temperature is
55~65 DEG C, composite dry powder microbial inoculum is made in crushed after being dried.
Composite dry powder microbial inoculum obtained can be used in the processing of Sludge in Oilfields.It can handle as follows containing greasy dirt
Mud:
1) oily sludge is added in open sludge-tank；
2) using wheat bran or attapulgite as bio-carrier, the composite dry powder bacterium of biological reinforced dose and claim 2 acquisition is added
Agent obtains band carrier microbial inoculum；Weight ratio with each component in carrier microbial inoculum is
Bio-carrier: composite dry powder microbial inoculum: biological reinforced dose=100:(0.09~0.11): (0.03~0.05)；
3) the band carrier microbial inoculum that step 2 obtains is added in sludge-tank, the weight proportion of addition is band carrier microbial inoculum: containing greasy dirt
Mud=1:(10~40)；
4) lasting 15~30 days Air Exposures are carried out to the sludge in sludge-tank, makes the oil in sludge by fully degraded.
Itself further improvement lies in that, described biological reinforced dose be potassium phosphate or potassium nitrate.The oil-containing being added in sludge-tank
Sludge is former mud of the dry mud oil-containing weight content less than 50%, and usually the weight content of water is no less than 50% in original mud.
Wheat bran or attapulgite are loose porous, have the good colloidal nature such as unique dispersion, high temperature resistant, salt resistance alkali and
Higher adsorption capacity, and there is certain plasticity, Soil ventilation water holding capacity is helped to improve, high activity oil removing microbial inoculum is built
The sludge organism processing system of standing improves soil texture, adds with the interaction process of soil particle and pollutant
Big porosity, is effectively improved greasy filth character, improves microorganism adaptability, thus deoiling of sludge bioprocess is greatly improved
Efficiency.
Sludge after reaction can be placed in biological plant complex repairation case, carry out 10~30 days plants and microbial associations
It repairs.
The utility model has the advantages that
1, it is suitable for the direct biodegrade of High water cut, high oil-containing, high salinity Sludge in Oilfields, environmental resistance is good.
2, the preparation method of microbial inoculum has feature easy to operate, low in cost, is suitble to large-scale production application.
3, the high activity oil removing microbial inoculum developed can be suitable for High water cut, high oil-containing, high salinity Sludge in Oilfields
Directly biodegrade, microbial activity is high, adhesive force is strong, treatment effeciency is high.Sludge organism toxicity is greatly lowered after processing, plants
Object beneficiating ingredient substantial increase, clump count reaches >=10 in sludge after processing8cfu/g。
Specific embodiment
Embodiment 1
In the multiple oily wastewaters of Jiangsu oilfield, sludge-tank, oily wastewater and mud sample are taken；Bacterium therein is cultivated in LB
Base, soda acid neutral environment, enrichment culture under the conditions of 25~30 DEG C, then separate, purify and screen, obtain two plant height activity oil removings
Bacterium is respectively as follows:
GWN-1: Sphingobacterium (Latin name is Sphingobacterium sp.) is preserved in Chinese microorganism strain guarantor
It hides administration committee's common micro-organisms collection (CGMCC), preservation date: on March 20th, 2017, deposit number are as follows: CGMCC
No. 13902；It is sequenced as shown in 1 in sequence table.
GWN-2: class Brevibacillus brevis (Latin name is Brevibacillus parabrevis) is preserved in China
Microbiological Culture Collection administration committee common micro-organisms collection (CGMCC), preservation date: on March 20th, 2017, preservation
Number are as follows: CGMCC No. 13903；It is sequenced as shown in 2 in sequence table.
The bacterium solution containing GWN-1 bacterial strain is taken, the bacterium solution containing GWN-2 bacterial strain, it is desirable that the viable count of every kind of bacterium solution is 109~
1011A/ml；Two kinds of bacterium solutions are respectively GWN-1 bacterium solution by volume: GWN-2 bacterium solution=1:2 carries out mixing and obtains mixed liquor.
Microorganism in the mixed liquor has preferable activity in oily sludge environmental system, while having with good
Greasy filth degradation can survive in floorboard with high oil content, high-moisture-contensludge sludge system, and activity is high.
Embodiment 2
Difference from example 1 is that two kinds of bacterium solutions by volume be respectively GWN-1 bacterium solution: GWN-2 bacterium solution=1:1.9 into
Row mixing obtains mixed liquor.
Embodiment 3
Two kinds of bacterium solutions are respectively GWN-1 bacterium solution by volume: GWN-2 bacterium solution=1:2.1 carries out mixing and obtains mixed liquor.
Embodiment 4
Composite dry powder microbial inoculum can be prepared using the oil removing microbial inoculum in embodiment 1, preparation method includes the following steps:
1) preparation of culture solution: being added peptone, NaCl in water, and the peptone additional amount is 10g/L, the additional amount of NaCl
For 10g/L, then sterilizes 30 minutes, be cooled to room temperature at 118~125 DEG C；
2) it is inoculated with: two kinds of bacterium solutions in embodiment 1 being seeded to culture solution respectively and are fermented, inoculum concentration are as follows: 0.01v/v；Hair
Ferment temperature: 28~32 DEG C；Fermentation time: 48 h；Air is passed through in fermentation process into culture solution, is passed through the gas of air per minute
Liquid volume ratio is 1:1.5；
3) filters pressing: after fermentation, fermentation liquid is pressed dry with filter press by plate and frame type filter-press, being allowed to moisture content weight content is
40~50%, filters pressing pressure is 0.2MPa；
4) drying and crushing: being removed remaining moisture content part by passing through boiling type drying tower, make moisture content weight content 8~10%,
Drying temperature is 55~65 DEG C, and composite dry powder microbial inoculum is made in crushed after being dried.
Embodiment 5
Composite dry powder microbial inoculum can be prepared using the oil removing microbial inoculum in embodiment 2, preparation method includes the following steps:
1) preparation of culture solution: being added peptone, NaCl in water, and the peptone additional amount is 9g/L, the additional amount of NaCl
For 11g/L, then sterilizes 25 minutes, be cooled to room temperature at 118~125 DEG C；
2) it is inoculated with: two kinds of bacterium solutions in embodiment 2 being seeded to culture solution respectively and are fermented, inoculum concentration are as follows: 0.01v/v；Hair
Ferment temperature: 28~32 DEG C；Fermentation time: 56 h；Air is passed through in fermentation process into culture solution, is passed through air per minute
Gas-liquid volume ratio is 1:0.8；
3) filters pressing: after fermentation, fermentation liquid being pressed dry with filter press, and being allowed to moisture content weight content is 40~50%, filters pressing pressure
For 0.15MPa；
4) drying and crushing: remaining moisture content part is removed by drying tower, makes moisture content weight content 8~10%, drying temperature is
55~65 DEG C, composite dry powder microbial inoculum is made in crushed after being dried.
Embodiment 6
Composite dry powder microbial inoculum can be prepared using the oil removing microbial inoculum in embodiment 3, preparation method includes the following steps:
1) preparation of culture solution: being added peptone, NaCl in water, and the peptone additional amount is 11g/L, the additional amount of NaCl
For 9g/L, then sterilizes 35 minutes, be cooled to room temperature at 118~125 DEG C；
2) it is inoculated with: two kinds of bacterium solutions in embodiment 3 being seeded to culture solution respectively and are fermented, inoculum concentration are as follows: 0.01v/v；Hair
Ferment temperature: 28~32 DEG C；Fermentation time: 40 h；Air is passed through in fermentation process into culture solution, is passed through the gas of air per minute
Liquid volume ratio is 1:3；
3) filters pressing: after fermentation, fermentation liquid being pressed dry with filter press, and being allowed to moisture content weight content is 40~50%, filters pressing pressure
For 0.25MPa；
4) drying and crushing: remaining moisture content part is removed by drying tower, makes moisture content weight content 8~10%, drying temperature is
55~65 DEG C, composite dry powder microbial inoculum is made in crushed after being dried.
Embodiment 7
Above-described embodiment 4 obtain composite dry powder microbial inoculum can be used in disposal of oily sludge, processing method as follows into
Row:
1) oily sludge is added in open sludge-tank；The oily sludge being added in sludge-tank is dry mud oil-containing weight content
30% former mud.
2) using wheat bran or attapulgite as bio-carrier, potassium phosphate is added and is obtained as biological reinforced dose and embodiment 4
Composite dry powder microbial inoculum obtain band carrier microbial inoculum；Weight ratio with each component in carrier microbial inoculum is
Bio-carrier: composite dry powder microbial inoculum: biological reinforced dose=100:0.09:0.05；
3) the band carrier microbial inoculum that previous step obtains is added in sludge-tank, the weight proportion of addition is band carrier microbial inoculum: oil-containing
Sludge=1:10；
4) lasting 15 days Air Exposures are carried out to sludge-tank, makes the oil in sludge by fully degraded.
Embodiment 8
Above-described embodiment 5 obtain composite dry powder microbial inoculum can be used in disposal of oily sludge, processing method as follows into
Row:
1) oily sludge is added in open sludge-tank；The oily sludge being added in sludge-tank is dry mud oil-containing weight content
49% former mud.
2) using wheat bran or attapulgite as bio-carrier, potassium nitrate is added and is obtained as biological reinforced dose and embodiment 5
Composite dry powder microbial inoculum obtain band carrier microbial inoculum；Weight ratio with each component in carrier microbial inoculum is
Bio-carrier: composite dry powder microbial inoculum: biological reinforced dose=100:0.11:0.03；
3) the band carrier microbial inoculum that previous step obtains is added in sludge-tank, the weight proportion of addition is band carrier microbial inoculum: oil-containing
Sludge=1:20；
4) lasting 25 days Air Exposures are carried out to sludge-tank, makes the oil in sludge by fully degraded.
Embodiment 9
Above-described embodiment 6 obtain composite dry powder microbial inoculum can be used in disposal of oily sludge, processing method as follows into
Row:
1) oily sludge is added in side or round open sludge-tank；The oily sludge is dry mud oil-containing weight content 15%
Former mud；Sludge pool volume 100m3；
2) using wheat bran or attapulgite as bio-carrier, the composite dry powder microbial inoculum of biological reinforced dose and the acquisition of embodiment 6 is added
Obtain band carrier microbial inoculum；Weight ratio with each component in carrier microbial inoculum is
Bio-carrier: composite dry powder microbial inoculum: biological reinforced dose=100:0.1:0.04；Biological reinforced dose is potassium phosphate or potassium nitrate；
3) the band carrier microbial inoculum that step 2 obtains is added in sludge-tank, the weight proportion of addition is band carrier microbial inoculum: containing greasy dirt
Mud=1:40；
4) lasting 30 days Air Exposures are carried out to sludge-tank using lower part aeration mode, makes the oil in sludge by fully degraded.
Sludge oil content≤0.3% after processing, oil removal efficiency is up to 98% or more；It further, can again will be after degradation reaction
Sludge, which is placed in microorganism-plant complex repairation case, carries out reparation in 20 days or so, so that sludge reparation becomes Nutrition Soil, place
Sludge organism toxicity is greatly lowered after reason, plant beneficiating ingredient substantial increase, after reparation in sludge clump count reach >=
108cfu/g。
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although referring to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features.It is all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Sequence table
<110>Sinopec Group
Sinopec Co., Ltd. Jiangsu Oil Field Branch
<120>Preparation method and use of a kind of oil removing microbial inoculum, its composite dry powder microbial inoculum
<160> 2
<170> SIPOSequenceListing 1.0
<210> 2
<211> 1387
<212> DNA
<213>Sphingobacterium (Sphingobacterium sp.)
<400> 2
agtcgaacgg gatccatttg ttagcttgct aacagatggt gagagtggcg cacgggtgcg 60
taacgcgtga gcaacctacc catatcaggg ggatagcccg aagaaattcg gattaacacc 120
gcataagact acgagatggc atcatcaagt agttaaatat ttataggata tggatgggct 180
cgcgtgacat tagctagttg gtgaggtaac ggctcaccaa ggcgacgatg tctaggggct 240
ctgagaggag aatcccccac actggtactg agacacggac cagactccta cgggaggcag 300
cagtaaggaa tattggtcaa tggacggaag tctgaaccag ccatgccgcg tgcaggatga 360
ctgccctatg ggttgtaaac tgcttttgtc gaggaataaa cctatctacg tgtagatagc 420
tgaatgtact cgaagaataa ggatcggcta actccgtgcc agcagccgcg gtaatacgga 480
ggatccgagc gttatccgga tttattgggt ttaaagggtg cgtaggcggc actttaagtc 540
aggggtgaaa gacggcagct caactgtcgc agtgcctttg atactgaagt gcttgaatgc 600
ggttgaagac ggcggaatga gacaagtagc ggtgaaatgc atagatatgt ctcagaacac 660
cgattgcgaa ggcagctgtc taagccgtta ttgacgctga tgcacgaaag cgtggggatc 720
gaacaggatt agataccctg gtagtccacg ccctaaacga tgataactcg atgtttgcga 780
tatacagtaa gcgtccaagc gaaagcgtta agttatccac ctggggagta cgcccgcaag 840
ggtgaaactc aaaggaattg acgggggccc gcacaagcgg aggagcatgt ggtttaattc 900
gatgatacgc gaggaacctt acccgggctt gaaagttact gaaggatgca gagacgcatc 960
cgtccttcgg gacaggaaac taggtgctgc atggctgtcg tcagctcgtg ccgtgaggtg 1020
ttgggttaag tcccgcaacg agcgcaaccc ctatgtttag ttgccagcat ttaaggtggg 1080
gactctaaac agactgcctg cgcaagcaga gaggaaggcg gggacgacgt caagtcatca 1140
tggcccttac gtccggggct acacacgtgc tacaatggat ggtacagcgg gcagctacat 1200
agcaatatga tgccaatctc gaaaagccat tcacagttcg gatcggggtc tgcaactcga 1260
ccccgtgaag ttggattcgc tagtaatcgc gtatcagcaa tgacgcggtg aatacgttcc 1320
cgggccttgt acacaccgcc cgtcaagcca tgaaagctgg gggtacctaa agcatgtaac 1380
cgcaagg 1387
<210> 2
<211> 1394
<212> DNA
<213>class Brevibacillus brevis (Brevibacillus parabrevis)
<400> 2
gtcgagcgag ggtcttcgga ccctagcggc ggacgggtga gtaacacgta ggcaacctgc 60
ctctcagacc gggataacat agggaaactt atgctaatac cggataggtt tttggattgc 120
atgatccgaa aagaaaagat ggcttcggct atcactggga gatgggcctg cggcgcatta 180
gctagttggt ggggtaacgg cctaccaagg cgacgatgcg tagccgacct gagagggtga 240
ccggccacac tgggactgag acacggccca gactcctacg ggaggcagca gtagggaatt 300
ttccacaatg gacgaaagtc tgatggagca acgccgcgtg aacgatgaag gtcttcggat 360
tgtaaagttc tgttgtcagg gacgaacacg tgccgttcga atagggcggt accttgacgg 420
tacctgacga gaaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc 480
aagcgttgtc cggatttatt gggcgtaaag cgcgcgcagg cggctatgta agtctggtgt 540
taaagcccgg agctcaactc cggttcgcat cggaaactgt gtagcttgag tgcagaagag 600
gaaagcggta ttccacgtgt agcggtgaaa tgcgtagaga tgtggaggaa caccagtggc 660
gaaggcggct ttctggtctg taactgacgc tgaggcgcga aagcgtgggg agcaaacagg 720
attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taggtgttgg gggtttcaat 780
accctcagtg ccgcagctaa cgcaataagc actccgcctg gggagtacgc tcgcaagagt 840
gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa 900
gcaacgcgaa gaaccttacc aggtcttgac atcccgctga ccgctctgga gacagagctt 960
cccttcgggg cagcggtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt 1020
tgggttaagt cccgcaacga gcgcaaccct tatctttagt tgccagcatt cagttgggca 1080
ctctagagag actgccgtcg acaagacgga ggaaggcggg gatgacgtca aatcatcatg 1140
ccccttatga cctgggctac acacgtgcta caatggttgg tacaacggga tgctacctcg 1200
cgaggggacg ccaatctctg aaaaccaatc tcagttcgga ttgtaggctg caactcgcct 1260
acatgaagtc ggaatcgcta gtaatcgcgg atcagcatgc cgcggtgaat acgttcccgg 1320
gccttgtaca caccgcccgt cacaccacgg gagtttgcaa cacccgaagt cggtgaggta 1380
accgcaagga gcca 1394
Claims (7)
1. a kind of oil removing microbial inoculum, it is characterised in that: the mixed liquor of two kinds of bacterium solutions containing GWN-1 bacterial strain, GWN-2 bacterial strain, every kind
The viable count of bacterium solution is 109~1011A/ml；Two kinds of bacterium solutions are respectively GWN-1 bacterium solution: GWN-2 bacterium solution=1:(1.9 by volume
~2.1) it is mixed；
Wherein,
GWN-1: Sphingobacterium is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms collection
(CGMCC), preservation date: on March 20th, 2017, deposit number are as follows: CGMCC No. 13902；
GWN-2: class Brevibacillus brevis is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms collection
(CGMCC), preservation date: on March 20th, 2017, deposit number are as follows: CGMCC No. 13903.
2. a kind of preparation method of the composite dry powder microbial inoculum of oil removing microbial inoculum, it is characterised in that include the following steps:
1) preparation of culture solution: being added peptone, NaCl in water, and the peptone additional amount is 9~11g/L, and NaCl's adds
Entering amount is 9~11g/L, then sterilizes 25~35 minutes, is cooled to room temperature at 118~125 DEG C；
2) it is inoculated with: two kinds of bacterium solutions in claim 1 being seeded to culture solution respectively and are fermented, inoculum concentration are as follows: 0.01v/v；
Fermentation temperature: 28~32 DEG C；Fermentation time: 40~56 h；Air is passed through in fermentation process into culture solution, is passed through sky per minute
The gas-liquid volume ratio of gas is 1:(0.8~3)；
3) filters pressing: after fermentation, by the fermentation liquid GWN-1 bacterium solution of two kinds of bacterium: GWN-2 bacterium solution according to volume ratio=1:(1.9~
2.1) mixed, then pressed dry with filter press, be allowed to moisture content weight content be 40~50%, filters pressing pressure be 0.15~
0.25MPa；
4) drying and crushing: remaining moisture content part is removed by drying tower, makes moisture content weight content 8~10%, drying temperature is
55~65 DEG C, composite dry powder microbial inoculum is made in crushed after being dried.
3. a kind of purposes of the composite dry powder microbial inoculum of oil removing microbial inoculum according to claim 2, it is characterised in that be used for oil field oil-containing
In the processing of sludge.
4. a kind of purposes of the composite dry powder microbial inoculum of oil removing microbial inoculum according to claim 2, it is characterised in that locate as follows
Manage oily sludge:
1) oily sludge is added in open sludge-tank；
2) using wheat bran or attapulgite as bio-carrier, the composite dry powder bacterium of biological reinforced dose and claim 2 acquisition is added
Agent obtains band carrier microbial inoculum；Weight ratio with each component in carrier microbial inoculum is
Bio-carrier: composite dry powder microbial inoculum: biological reinforced dose=100:(0.09~0.11): (0.03~0.05)；
3) the band carrier microbial inoculum that step 2 obtains is added in sludge-tank, the weight proportion of addition is band carrier microbial inoculum: containing greasy dirt
Mud=1:(10~40)；
4) lasting 15~30 days Air Exposures are carried out to the sludge in sludge-tank, makes the oil in sludge by fully degraded.
5. a kind of purposes of the composite dry powder microbial inoculum of oil removing microbial inoculum according to claim 4, it is characterised in that the biology
Hardening agent is potassium phosphate or potassium nitrate.
6. a kind of purposes of the composite dry powder microbial inoculum of oil removing microbial inoculum according to claim 4, it is characterised in that in step 1)
The oily sludge being added in sludge-tank is former mud of the dry mud oil-containing weight content less than 50%.
7. a kind of purposes of the composite dry powder microbial inoculum of oil removing microbial inoculum according to claim 4, it is characterised in that in step 4)
Air Exposure is carried out to sludge-tank using lower part aeration mode.
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