CN109158137B - Preparation method of micro-fluidic chip - Google Patents
Preparation method of micro-fluidic chip Download PDFInfo
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- CN109158137B CN109158137B CN201811258313.1A CN201811258313A CN109158137B CN 109158137 B CN109158137 B CN 109158137B CN 201811258313 A CN201811258313 A CN 201811258313A CN 109158137 B CN109158137 B CN 109158137B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
Abstract
The invention discloses a preparation method of a microfluidic chip, which is prepared by adding carbon nano materials before forming a substrate and a cover plate and then bonding. The invention adds the carbon nano material into the raw materials before the base sheet and the cover sheet are molded, so that the carbon nano material and the macromolecular micro-fluidic chip are mixed into a whole, the bonding strength is high, and the surface microstructure and the surface functional property of the micro-fluidic chip are greatly changed. The carbon nano material has strong hydrophobicity and ultrahigh specific surface area, so when water is used as a disperse phase, the addition of the carbon nano material is easier to form liquid drops.
Description
Technical Field
The invention relates to the technical field of micro-droplets, in particular to a preparation process of a micro-fluidic chip easy to generate droplets.
Background
The micro-droplet technology, an important branch of the micro-fluidic chip technology, is due to the immiscible nature of the two solutions in the micro-scale channel. The micro-nano technology is characterized in that one solution is used as a dispersed phase, the other solution is used as a continuous phase, and the dispersed phase is sheared into micro droplets with nano-scale and lower volumes by utilizing the interaction between the flow shearing force and the surface tension by combining the micro-fluidic chip material, the structure of a micro-fluidic chip channel and the external force control action.
The core of the micro-droplet technology is also the first step of generating micro-droplets, so that the key of the micro-droplet technology is to manufacture a micro-fluidic chip which is beneficial to generating the micro-droplets. The generation of micro-droplets has a great relationship with the properties of the structure, the hydrophilicity and the hydrophobicity, the specific surface area and the like of the micro-fluidic chip channel.
At present, the surface coating method is mostly adopted in the technology for changing the surface wetting property of the microfluidic chip channel or a surfactant is added into a processing target, on one hand, special instrument and equipment are needed, the operation is complicated, the cost is higher, and on the other hand, the conditions of uneven coating and low bonding strength between the coating and the surface of the microfluidic chip can also occur. The method for changing the wettability of the microfluidic chip channel by adopting the surface coating and introducing the surfactant needs to introduce a chemical reagent, so that the problem of reagent pollution exists, and interference is brought to subsequent application.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a method for preparing a microfluidic chip, which has the advantages of simple process, no reagent pollution and capability of effectively generating micro liquid drops.
In order to achieve the above object, the present invention provides a method for preparing a microfluidic chip, comprising the steps of:
(1) preparation of a substrate: adding carbon nano material into the raw material liquid high molecular material and the curing agent to prepare a substrate;
(2) preparation of cover slip: adding carbon nano material into the raw material liquid high molecular material and the curing agent to prepare a cover plate;
(3) preparing a micro-fluidic chip: bonding the substrate prepared in the step (1) and the cover plate prepared in the step (2) to obtain the microfluidic chip;
wherein, the carbon nano material in the step (1) is one or a mixture of more of graphene, carbon nano tubes and carbon quantum dots; the carbon nano material in the step (2) is one or a mixture of more of graphene, carbon nano tubes and carbon quantum dots; in the substrate prepared in the step (1), the content of the carbon nano material is 0.005-0.1 wt%; and (3) in the cover plate prepared in the step (2), the content of the carbon nano material is 0.005-0.1 wt%.
Further, the substrate in step (1) can be prepared by molding, hot pressing, LIGA technique or laser ablation; the cover plate in the step (2) can be prepared by adopting a molding method, a hot pressing method, a LIGA technology or a laser ablation method.
The preparation of the base sheet and the cover sheet can be carried out by adopting a molding method, and the specific steps are as follows:
(1) preparation of a substrate: uniformly stirring a PDMS precursor and a curing agent in a mass ratio of 10:1, adding a carbon nano material into the PDMS precursor and the curing agent, uniformly stirring, degassing, pouring, curing and demolding to prepare a substrate with a microchannel; in the substrate, the content of the carbon nano material is 0.005-0.1 wt%;
(2) preparation of cover slip: uniformly stirring a PDMS precursor and a curing agent in a mass ratio of 10:1, adding a carbon nano material into the mixture, uniformly stirring, degassing, pouring, curing and demolding to prepare a cover plate; the content of the carbon nano material in the cover plate is 0.005wt% -0.1 wt%.
(3) Preparing a micro-fluidic chip: and (3) bonding the substrate prepared in the step (1) and the cover plate prepared in the step (2) to obtain the microfluidic chip.
The preparation of the base sheet and the cover sheet can be carried out by adopting a hot pressing method, and the preparation method comprises the following specific steps:
(1) preparation of a substrate: dissolving a PMMA precursor and an initiator in DMF (dimethyl formamide) to prepare a uniform PMMA/DMF solution, dissolving a carbon nano material in the DMF solution to obtain a uniform carbon nano material/DMF solution, mixing the PMMA/DMF solution and the carbon nano material/DMF solution, pouring the mixture into a watch glass, and preparing a substrate with a microchannel by a hot pressing method after the solvent is completely volatilized and the PMMA/carbon nano material is cured and molded; the content of the carbon nano material in the substrate is 0.005-0.1 wt%;
(2) preparation of cover slip: : dissolving a PMMA precursor and an initiator in DMF to prepare a uniform PMMA/DMF solution, dissolving a carbon nano material in the DMF solution to obtain a uniform carbon nano material/DMF solution, mixing the PMMA/DMF solution and the carbon nano material/DMF solution, pouring the mixture into a surface dish, and curing and molding the PMMA/carbon nano material after the solvent is completely volatilized to prepare a cover plate; the content of the carbon nano material in the cover plate is 0.005-0.1 wt%;
(3) preparing a micro-fluidic chip: and (3) bonding the substrate prepared in the step (1) and the cover plate prepared in the step (2) to obtain the microfluidic chip.
The preparation process and raw material selection of the base sheet and the cover sheet can be different, and the specific steps are as follows:
(1) preparation of a substrate: dissolving a PMMA precursor and an initiator in DMF (dimethyl formamide) to prepare a uniform PMMA/DMF solution, dissolving a carbon nano material in the DMF solution to obtain a uniform carbon nano material/DMF solution, mixing the PMMA/DMF solution and the carbon nano material/DMF solution, pouring the mixture into a watch glass, and preparing a substrate with a microchannel by a hot pressing method after the solvent is completely volatilized and the PMMA/carbon nano material is cured and molded; the content of the carbon nano material in the substrate is 0.005-0.1 wt%;
(2) preparation of cover slip: uniformly stirring a PDMS precursor and a curing agent in a mass ratio of 10:1, adding a carbon nano material into the mixture, uniformly stirring, degassing, pouring, curing and demolding to prepare a cover plate; the content of the carbon nano material in the cover plate is 0.005-0.1 wt%;
(3) preparing a micro-fluidic chip: and (3) bonding the substrate prepared in the step (1) and the cover plate prepared in the step (2) to obtain the microfluidic chip.
Compared with the prior art, the invention has the following advantages:
1. the invention adds the carbon nano material into the raw materials before the base sheet and the cover sheet are molded, so that the carbon nano material and the macromolecular micro-fluidic chip are mixed into a whole, the bonding strength is high, and the surface microstructure and the surface functional property of the micro-fluidic chip are greatly changed. The carbon nano material has strong hydrophobicity and ultrahigh specific surface area, so when water is used as a disperse phase, the addition of the carbon nano material is easier to form liquid drops.
2. The invention adopts high molecular polymer as the main material of the microfluidic chip, has the advantages of capability of copying the microfluidic chip with high fidelity by a molding method, durability, certain chemical inertia, simple operation, low price, no toxicity and the like, and is very suitable for manufacturing the disposable microfluidic chip in a large scale. And the high molecular polymer has good optical properties, so that the subsequent detection application is facilitated.
3. The preparation process of the microfluidic chip is simple, easy to operate, convenient and quick, greatly simplifies the operation steps and saves the experiment time. And chemical reagents such as surfactants and the like are not required to be introduced in the whole preparation process, so that the pollution of the reagents is avoided, and the preparation method is green and environment-friendly.
Drawings
Fig. 1 is a flow chart of a process for preparing a microfluidic chip modified by a carbon nanomaterial in embodiments 1 to 3 of the present invention;
FIG. 2 is a diagram showing the experimental effect of the microfluidic chip prepared by modifying the carbon nanomaterial according to the present invention;
fig. 3 is a graph showing experimental effects of a conventional microfluidic chip without modification by adding a carbon nanomaterial.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Example 1
As shown in fig. 1, the preparation process of the microfluidic chip modified by the carbon nanomaterial comprises the following steps:
(1) weighing: weighing the PDMS precursor and the curing agent in a mass ratio of 10:1 in a beaker, and uniformly stirring by using a glass rod.
(2) Adding carbon nano materials: and (2) adding graphene into the beaker in the step (1) to enable the content of the graphene to be 0.005wt%, and uniformly stirring.
(3) Degassing: and (3) putting the beaker obtained in the step (2) into a vacuum drying oven, and turning on a vacuum pump to prevent the PDMS in the beaker from overflowing until the air in the PDMS is completely exhausted.
(4) Pouring: and (4) taking out the PDMS liquid obtained in the step (3) and pouring the PDMS liquid on the chip male mold, and placing the chip male mold in a container with a groove in advance in order to prevent the PDMS liquid from flowing out. During pouring, the pouring is carried out from the middle to the periphery, so that bubbles are avoided.
(5) And (3) curing: and (4) putting the container in the step (4) into a 40 ℃ oven for heating for 2h, taking out the container after beginning to heat for 10min, observing whether bubbles exist, puncturing the bubbles by using a needle, and then continuously putting the container into the oven for heating.
(6) Making a cover plate: and (4) pouring the emptied PDMS liquid on a clean blank culture medium according to the method of the step (1-3), and curing in the same step (5).
(7) Bonding: and (3) upward bonding surfaces of the substrate uncovered from the male die and the cover plate uncovered from the clean culture dish, and adjusting the voltage of a laboratory corona treater to enable an electrode to generate purple corona, wherein the distance between the electrode and the microfluidic chip is 4-5 mm, and the treatment time is 1 min. And bonding and pressing the two bonded surfaces within 1min, and putting the bonded surfaces into a 100 ℃ oven to be heated for 1h to obtain the microfluidic chip.
Comparative example 1
The preparation process of the micro-fluidic chip without carbon nano material modification by adopting the conventional process comprises the following steps:
(1) weighing: weighing the PDMS precursor and the curing agent in a mass ratio of 10:1 in a beaker, and uniformly stirring by using a glass rod.
(2) Degassing: and (3) putting the beaker obtained in the step (1) into a vacuum drying oven, and turning on a vacuum pump to prevent the PDMS in the beaker from overflowing until the air in the PDMS is completely exhausted.
(3) Pouring: and (3) taking out the PDMS liquid obtained in the step (2) and pouring the PDMS liquid on the chip male mold, and placing the chip male mold in a container with a groove in advance in order to prevent the PDMS liquid from flowing out. During pouring, the pouring is carried out from the middle to the periphery, so that bubbles are avoided.
(4) And (3) curing: and (4) putting the container in the step (3) into a 40 ℃ oven for heating for 2h, taking out the container after beginning to heat for 10min, observing whether bubbles exist, puncturing the bubbles by using a needle, and then continuously putting the container into the oven for heating.
(5) Making a cover plate: pouring the emptied PDMS liquid on a clean blank culture medium according to the method of the step 1-2, and curing in the same step (4).
(6) Bonding: and (3) upward bonding surfaces of the substrate uncovered from the male die and the cover plate uncovered from the clean culture dish, and adjusting the voltage of a laboratory corona treater to enable an electrode to generate purple corona, wherein the distance between the electrode and the microfluidic chip is 4-5 mm, and the treatment time is 1 min. And bonding and pressing the two bonded surfaces within 1min, and heating in an oven at 100 ℃ for 1h to obtain the microfluidic chip of the comparative example.
Comparative examples
Experiments were conducted separately on the samples prepared in example 1 and comparative example 1, with silicone oil being the continuous phase and rhodamine B dye being the dispersed phase. The continuous phase and the dispersed phase of the two experiments have no change, the experiments adopt micro-injection pumps to drive fluid to enter a micro-channel, and the flow rates of all the micro-injection pumps are the same. The flow rate of the micropump connected to the branch inlet is 0.1-0.6. mu.L/m, and the flow rate of the microinjection pump connected to the continuous phase inlet is 1-2. mu.L/m. The experimental result shows that the microfluidic chip added with the carbon nanomaterial in example 1 can generate single and uniformly dispersed droplets, while the microfluidic chip without the carbon nanomaterial in comparative example 1 cannot generate droplets. The experimental results are shown in fig. 2 and 3.
According to the experimental effect, the experimental results shown in fig. 2 and 3 show that the micro-fluidic chip preparation process adopted by the invention effectively promotes the generation of micro-droplets and is simple and convenient to operate.
Comparative example 2
The preparation process of the micro-fluidic chip modified by the carbon nano material comprises the following steps:
(1) weighing: weighing the PDMS precursor and the curing agent in a mass ratio of 10:1 in a beaker, and uniformly stirring by using a glass rod.
(2) Adding carbon nano materials: and (2) adding graphene into the beaker in the step (1) to enable the content of the graphene to be 0.004wt%, and uniformly stirring.
(3) Degassing: and (3) putting the beaker obtained in the step (2) into a vacuum drying oven, and turning on a vacuum pump to prevent the PDMS in the beaker from overflowing until the air in the PDMS is completely exhausted.
(4) Pouring: and (4) taking out the PDMS liquid obtained in the step (3) and pouring the PDMS liquid on the chip male mold, and placing the chip male mold in a container with a groove in advance in order to prevent the PDMS liquid from flowing out. During pouring, the pouring is carried out from the middle to the periphery, so that bubbles are avoided.
(5) And (3) curing: and (4) putting the container in the step (4) into a 40 ℃ oven for heating for 2h, taking out the container after beginning to heat for 10min, observing whether bubbles exist, puncturing the bubbles by using a needle, and then continuously putting the container into the oven for heating.
(6) Making a cover plate: and (4) pouring the emptied PDMS liquid on a clean blank culture medium according to the method of the step (1-3), and curing in the same step (5).
(7) Bonding: and (3) upward bonding surfaces of the substrate uncovered from the male die and the cover plate uncovered from the clean culture dish, and adjusting the voltage of a laboratory corona treater to enable an electrode to generate purple corona, wherein the distance between the electrode and the microfluidic chip is 4-5 mm, and the treatment time is 1 min. And bonding and pressing the two bonded surfaces within 1min, and heating in an oven at 100 ℃ for 1h to obtain the microfluidic chip of the comparative example.
The microfluidic chip prepared by the steps cannot generate liquid drops, and the effect is as shown in figure 3 in the comparative example.
Comparative example 3
The preparation process of the micro-fluidic chip modified by the carbon nano material comprises the following steps:
(1) weighing: weighing the PDMS precursor and the curing agent in a mass ratio of 10:1 in a beaker, and uniformly stirring by using a glass rod.
(2) Adding carbon nano materials: and (2) adding graphene into the beaker in the step (1) to enable the content of the graphene to be 0.110wt%, and uniformly stirring.
(3) Degassing: and (3) putting the beaker obtained in the step (2) into a vacuum drying oven, and turning on a vacuum pump to prevent the PDMS in the beaker from overflowing until the air in the PDMS is completely exhausted.
(4) Pouring: and (4) taking out the PDMS liquid obtained in the step (3) and pouring the PDMS liquid on the chip male mold, and placing the chip male mold in a container with a groove in advance in order to prevent the PDMS liquid from flowing out. During pouring, the pouring is carried out from the middle to the periphery, so that bubbles are avoided.
(5) And (3) curing: and (4) putting the container in the step (4) into a 40 ℃ oven for heating for 2h, taking out the container after beginning to heat for 10min, observing whether bubbles exist, puncturing the bubbles by using a needle, and then continuously putting the container into the oven for heating.
(6) Making a cover plate: and (4) pouring the emptied PDMS liquid on a clean blank culture medium according to the method of the step (1-3), and curing in the same step (5).
(7) Bonding: and (3) upward bonding surfaces of the substrate uncovered from the male die and the cover plate uncovered from the clean culture dish, and adjusting the voltage of a laboratory corona treater to enable an electrode to generate purple corona, wherein the distance between the electrode and the microfluidic chip is 4-5 mm, and the treatment time is 1 min. And bonding and pressing the two bonded surfaces within 1min, and heating in an oven at 100 ℃ for 1h to obtain the microfluidic chip of the comparative example.
The micro-fluidic chip prepared by adopting the steps is difficult to observe the formation of liquid drops due to the overhigh content of the carbon nano material, and the high content of the carbon nano material increases the cost of the micro-fluidic chip, so that the micro-fluidic chip is not suggested to be adopted.
Example 2
As shown in fig. 1, the preparation process of the microfluidic chip modified by the carbon nanomaterial comprises the following steps:
(1) weighing: weighing the PDMS precursor and the curing agent in a mass ratio of 10:1 in a beaker, and uniformly stirring by using a glass rod.
(2) Adding carbon nano materials: and (2) adding the carbon nano tube, the graphene and the carbon quantum dot into the beaker in the step (1) to enable the contents of the carbon nano tube, the graphene and the carbon quantum dot to be 0.005wt%, and uniformly stirring.
(3) Degassing: and (3) putting the beaker obtained in the step (2) into a vacuum drying oven, and turning on a vacuum pump to prevent the PDMS in the beaker from overflowing until the air in the PDMS is completely exhausted.
(4) Pouring: and (4) taking out the PDMS liquid obtained in the step (3) and pouring the PDMS liquid on the chip male mold, and placing the chip male mold in a container with a groove in advance in order to prevent the PDMS liquid from flowing out. During pouring, the pouring is carried out from the middle to the periphery, so that bubbles are avoided.
(5) And (3) curing: and (4) putting the container in the step (4) into a 75 ℃ oven for heating for 1h, taking out the container after beginning to heat for 10min, observing whether bubbles exist, puncturing the bubbles by using a needle, and then continuously putting the container into the oven for heating.
(6) Making a cover plate: and (4) pouring the emptied PDMS liquid on a clean blank culture medium according to the method of the step (1-3), and curing in the same step (5).
(7) Bonding: and (3) upward bonding surfaces of the substrate uncovered from the male die and the cover plate uncovered from the clean culture dish, and adjusting the voltage of a laboratory corona treater to enable an electrode to generate purple corona, wherein the distance between the electrode and the microfluidic chip is 4-5 mm, and the treatment time is 1 min. And bonding and pressing the two bonded surfaces within 1min, and putting the bonded surfaces into a 100 ℃ oven to be heated for 1h to obtain the microfluidic chip.
The microfluidic chip prepared by the steps can generate single and uniformly dispersed liquid drops, and the effect is as shown in the figure 2 in the embodiment with the same effect.
Example 3
As shown in fig. 1, the preparation process of the microfluidic chip modified by the carbon nanomaterial comprises the following steps:
(1) weighing: weighing the PDMS precursor and the curing agent in a mass ratio of 10:1 in a beaker, and uniformly stirring by using a glass rod.
(2) Adding carbon nano materials: adding carbon quanta into the beaker in the step (1) to enable the content of the carbon quanta to be 0.1wt%, and uniformly stirring.
(3) Degassing: and (3) putting the beaker obtained in the step (2) into a vacuum drying oven, and turning on a vacuum pump to prevent the PDMS in the beaker from overflowing until the air in the PDMS is completely exhausted.
(4) Pouring: and (4) taking out the PDMS liquid obtained in the step (3) and pouring the PDMS liquid on the chip male mold, and placing the chip male mold in a container with a groove in advance in order to prevent the PDMS liquid from flowing out. During pouring, the pouring is carried out from the middle to the periphery, so that bubbles are avoided.
(5) And (3) curing: and (4) putting the container in the step (4) into a 90 ℃ oven for heating for 20min, taking out the container after heating for 10min, observing whether bubbles exist or not, pricking the bubbles with a needle, and then continuously putting the container into the oven for heating.
(6) Making a cover plate: and (4) pouring the emptied PDMS liquid on a clean blank culture medium according to the method of the step (1-3), and curing in the same step (5).
(7) Bonding: and (3) upward bonding surfaces of the substrate uncovered from the male die and the cover plate uncovered from the clean culture dish, and adjusting the voltage of a laboratory corona treater to enable an electrode to generate purple corona, wherein the distance between the electrode and the microfluidic chip is 4-5 mm, and the treatment time is 1 min. And bonding and pressing the two bonded surfaces within 1min, and putting the bonded surfaces into a 100 ℃ oven to be heated for 1h to obtain the microfluidic chip.
The microfluidic chip prepared by the steps can generate single and uniformly dispersed liquid drops, and the effect is as shown in the figure 2 in the embodiment with the same effect.
Example 4
The preparation process of the micro-fluidic chip modified by the carbon nano material comprises the following steps:
(1) weighing: a proper amount of PMMA precursor and initiator (the proportion of which is selected according to the conventional process) are weighed and dissolved in Dimethylformamide (DMF) to obtain a uniform PMMA/DMF solution.
(2) Adding carbon nano materials: and (2) dissolving Graphene (GO) accounting for 0.005wt% of the total amount of the raw materials in DMF to prepare a GO/DMF solution, adding the GO/DMF solution into the PMMA/DMF solution obtained in the step (1), and uniformly stirring to prepare a GO/PMMA/DMF solution.
(3) Curing the GO/PMMA composite material: and (3) pouring the GO/PMMA/DMF solution obtained in the step (2) into a surface dish, heating for 2d on an electric heating plate at the temperature of 45 ℃, and obtaining the solidified GO/PMMA composite material after the DMF solution slowly volatilizes completely.
(4) Manufacturing a substrate: and preparing the PMMA substrate with the micro-channel by using a hot pressing method. The method comprises the specific steps of heating a PMMA substrate to 106 ℃ in a hot pressing device, applying certain pressure (the area of 4 inches is applied with force by 20-30 kN) on a male die, keeping for 30-60 s, and then cooling and demoulding the male die and the substrate together under the pressurizing condition to obtain the PMMA substrate with the micro-channel.
(5) Making a cover plate: preparing the GO/PMMA composite material cover plate according to the method in the step 1-3.
(6) Bonding: and (3) attaching and pressing the PMMA substrate with the micro-channel and the cover plate in the step (5), and placing the PMMA substrate and the cover plate in a 105 ℃ oven for heat preservation for 5min to obtain the GO/PMMA composite material micro-fluidic chip.
The microfluidic chip prepared by the steps can generate single and uniformly dispersed liquid drops, and the effect is as shown in the figure 2 in the embodiment with the same effect.
Example 5
The preparation process of the micro-fluidic chip modified by the carbon nano material comprises the following steps:
(1) manufacturing a substrate: a GO/PMMA composite substrate with channels was prepared as described in alternative example 4.
(2) Making a cover plate: the CQDS/PDMS composite coverslip prepared in example 3 was selected.
(3) Bonding: and (4) enabling the bonding surface of the cover plate in the step (5) to face upwards, adjusting the voltage of a laboratory corona treater to enable an electrode to generate purple corona, wherein the distance between the electrode and the microfluidic chip is 4-5 mm, and the treatment time is 1 min. And attaching and pressing the cover plate subjected to corona treatment with a PMMA substrate with a micro-channel within 1min, and heating in a 75 ℃ oven for 10min to obtain the micro-fluidic chip with the substrate made of GO/PMMA composite material and the cover plate made of CQDS/PDMS composite material.
The microfluidic chip prepared by the steps can generate single and uniformly dispersed liquid drops, and the effect is as shown in the figure 2 in the embodiment with the same effect.
Claims (5)
1. A method for preparing a microfluidic chip is characterized by comprising the following steps: the method comprises the following steps:
(1) preparation of a substrate: adding carbon nano material into the raw material liquid high molecular material and the curing agent to prepare a substrate;
(2) preparation of cover slip: adding carbon nano material into the raw material liquid high molecular material and the curing agent to prepare a cover plate;
(3) preparing a micro-fluidic chip: bonding the substrate prepared in the step (1) and the cover plate prepared in the step (2) to obtain the microfluidic chip;
the carbon nano material in the step (1) is one or a mixture of Graphene (GO), Carbon Nano Tubes (CNT) and Carbon Quantum Dots (CQDS); the carbon nano material in the step (2) is one or a mixture of graphene, carbon nano tubes and carbon quantum dots; in the substrate prepared in the step (1), the content of the carbon nano material is 0.005-0.1 wt%; the content of the carbon nano material in the cover plate prepared in the step (2) is 0.005-0.1 wt%.
2. The method of claim 1, wherein: the preparation of the substrate in the step (1) adopts a molding method, a hot pressing method, a LIGA technology or a laser ablation method; and (3) preparing the cover plate in the step (2) by adopting a molding method, a hot pressing method, a LIGA technology or a laser ablation method.
3. The method of claim 2, wherein: the method comprises the following steps:
(1) preparation of a substrate: uniformly stirring a PDMS precursor and a curing agent in a mass ratio of 10:1, adding a carbon nano material into the PDMS precursor and the curing agent, uniformly stirring, degassing, pouring, curing and demolding to prepare a substrate with a microchannel; the content of the carbon nano material in the substrate is 0.005-0.1 wt%;
(2) preparation of cover slip: uniformly stirring a PDMS precursor and a curing agent in a mass ratio of 10:1, adding a carbon nano material into the mixture, uniformly stirring, degassing, pouring, curing and demolding to prepare a cover plate; the content of the carbon nano material in the cover plate is 0.005-0.1 wt%;
(3) preparing a micro-fluidic chip: and (3) bonding the substrate prepared in the step (1) and the cover plate prepared in the step (2) to obtain the microfluidic chip.
4. The method of claim 2, wherein: the method comprises the following steps:
(1) preparation of a substrate: dissolving a PMMA precursor and an initiator in Dimethylformamide (DMF) to prepare a uniform PMMA/DMF solution, dissolving a carbon nano material in the DMF solution to obtain a uniform carbon nano material/DMF solution, mixing the PMMA/DMF solution and the carbon nano material/DMF solution, pouring the mixture into a watch glass, and preparing a substrate with a microchannel by a hot pressing method after the solvent is completely volatilized and the PMMA/carbon nano material is cured and molded; the content of the carbon nano material in the substrate is 0.005-0.1 wt%;
(2) preparation of cover slip: dissolving a PMMA precursor and an initiator in DMF to prepare a uniform PMMA/DMF solution, dissolving a carbon nano material in the DMF solution to obtain a uniform carbon nano material/DMF solution, mixing the PMMA/DMF solution and the carbon nano material/DMF solution, pouring the mixture into a surface dish, and curing and molding the PMMA/carbon nano material after the solvent is completely volatilized to prepare a cover plate; the content of the carbon nano material in the cover plate is 0.005-0.1 wt%;
(3) preparing a micro-fluidic chip: and (3) bonding the substrate prepared in the step (1) and the cover plate prepared in the step (2) to obtain the microfluidic chip.
5. The method of claim 2, wherein: the method comprises the following steps:
(1) preparation of a substrate: dissolving a PMMA precursor and an initiator in DMF (dimethyl formamide) to prepare a uniform PMMA/DMF solution, dissolving a carbon nano material in the DMF solution to obtain a uniform carbon nano material/DMF solution, mixing the PMMA/DMF solution and the carbon nano material/DMF solution, pouring the mixture into a watch glass, and preparing a substrate with a microchannel by a hot pressing method after the solvent is completely volatilized and the PMMA/carbon nano material is cured and molded; the content of the carbon nano material in the substrate is 0.005-0.1 wt%;
(2) preparation of cover slip: uniformly stirring a PDMS precursor and a curing agent in a mass ratio of 10:1, adding a carbon nano material into the mixture, uniformly stirring, degassing, pouring, curing and demolding to prepare a cover plate; the carbon nano material accounts for 0.005-0.1 wt% of the total amount of the PDMS precursor, the curing agent and the carbon nano material;
(3) preparing a micro-fluidic chip: and (3) bonding the substrate prepared in the step (1) and the cover plate prepared in the step (2) to obtain the microfluidic chip.
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