CN109125809A - To inhibit bacterial growth and promote the titanium surface and preparation method thereof of protein adherence - Google Patents
To inhibit bacterial growth and promote the titanium surface and preparation method thereof of protein adherence Download PDFInfo
- Publication number
- CN109125809A CN109125809A CN201810951824.5A CN201810951824A CN109125809A CN 109125809 A CN109125809 A CN 109125809A CN 201810951824 A CN201810951824 A CN 201810951824A CN 109125809 A CN109125809 A CN 109125809A
- Authority
- CN
- China
- Prior art keywords
- titanium
- preparation
- titanium sheet
- bacterial growth
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/30—Inorganic materials
- A61L27/306—Other specific inorganic materials not covered by A61L27/303 - A61L27/32
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/08—Materials for coatings
- A61L31/082—Inorganic materials
- A61L31/088—Other specific inorganic materials not covered by A61L31/084 or A61L31/086
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/16—Biologically active materials, e.g. therapeutic substances
-
- C—CHEMISTRY; METALLURGY
- C25—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES; APPARATUS THEREFOR
- C25D—PROCESSES FOR THE ELECTROLYTIC OR ELECTROPHORETIC PRODUCTION OF COATINGS; ELECTROFORMING; APPARATUS THEREFOR
- C25D11/00—Electrolytic coating by surface reaction, i.e. forming conversion layers
- C25D11/02—Anodisation
- C25D11/26—Anodisation of refractory metals or alloys based thereon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
Abstract
The invention discloses to inhibit bacterial growth and promote the titanium surface and preparation method thereof of protein adherence.Pass through the mask of customization various sizes and figure first, then titanium plate surface is cleaned, anodic oxidation, annealing, hydrophobization, ultraviolet lighting technology, make a kind of Nano tube array of titanium dioxide that height specification is orderly of smooth Surface Creation, while breaking forth the controllable parents surface of precision in Nano tube array of titanium dioxide glazing, one kind, which has been made, can inhibit bacterial growth, it can promote protein adherence again, while the titanium surface of regulation protein adhesion locations can be oriented again.This method can prepare the titanium sheet combined with arbitrary shape micron pattern and indefinite form nano-pattern in titanium sheet, there is inhibition bacterial growth to assign titanium sheet, promote the function of protein adherence, while the adhesion locations of regulation protein can be oriented again, offers reference for design man-made organ.
Description
Technology neighborhood
The invention belongs to Bionic medicine technical fields, and in particular to inhibit bacterial growth and promote the titanium table of protein adherence
Face and preparation method thereof.
Background technique
In field of biomedicine, central venous catheter and Needleless connector, endotracheal tube, intrauterine device, the mechanical heart
Dirty valve, pacemaker, peritoneal dialysis catheters, Tympanostomy pipe, sounding artificial limb, orthopaedic joint prosthesis and percutaneous orthopedic dress
(including external fixator and bone anchor amputation artificial limb) etc. is set, more and more artificial joint replacements change for mitigating patient's pain
Philanthropists' quality of the life.
Titanium have with the good compatibility of human body cell, thus for however transplanting in the transfer operation of man-made organ
The reason of operative failure is often resulted in operation is exactly that biomaterial is infected by bacterial, once bacterium is integrated to biomaterial table
Face, function will change.More specifically, gene expression changes, and growth rate changes, and host defense mechanism no longer can
It is enough to remove them from internal, and the biomembrane of antibiotic-resistant is formed, the development of this biomembrane is to cause many chronic senses
The reason of dye.
In addition, antibiotics resistance bacterial strain with antibiotic treatment cannot be used after surface adhesion.It is common more in clinical orthopaedics
Kind drug-fast bacteria includes staphylococcus aureus and staphylococcus epidermis.Accordingly, it is believed that preventing bacterial adhesion (without using medicine
Object) it may be one of the best way, to reduce orthopaedics implant infection.Application number 201510024331.3 discloses a kind of table
Face micro-patterning antimicrobial surface antibacterial film utilizes electron beam lithography, the removing of electron beam deposition glue, reactive ion etching, hotting mask pressure
A series of micro-processing technologies such as print, production surface have polyethylene, polystyrene, the polyethylene terephthalate of micro-nano structure
The materials such as vinegar, polylactic acid.Manufactured micro-nano structure includes rice font, stripe, dot matrix type, and demonstrates the film of this class formation
Material has good anti-microbial property.
After environment contacts in implant and living organism, first happens is that protein is in the non-specific of implant surfaces
Property absorption, be broadly divided into three processes: protein molecule in body fluid to material surface spread it is close, attach and structural rearrangement after
Desorption.With the continuous deepening of research, construct on the surface of the material in recent years pattern structure (or topological structure) with observe with
The interaction of protein becomes hot spot.Firstly, the pattern structure that material surface is constructed directly affects roughness and surface
Product, this will provide more adsorption sites and proteins interaction；Secondly, the geometric dimension and shape of pattern can be to not
It is made a significant impact with the absorption quantity of protein species and variation of conformation etc..The protein molecular of different sizes and shape
It is capable of the patterning on specifically identification material surface, i.e., is selected along the width of certain geometric dimension and the groove of depth
Adsorb to property.
Titanium surface TiO2The building of nanotube micro-patterning not only increases the roughness of original surface, more creates multistage
Roughness, and then the adsorption capacity of protein is largely improved, in the fusion process for promoting implantation material and surrounding tissue
In, titanium surface hydrophilic part is preferentially adhered to by histocyte, and hydrophobic part has good antibacterial adhesive capacity, to effectively press down
The adherency growth of surrounding bacterial is made.
Prevent bacterial growth and the peculiar surface texture of promotion protein adherence from just seeming by preparing to have in titanium sheet
Particularly important, the present invention is prepared on the surface of titanium sheet with strip and netted micro- by method that ultraviolet catalytic etches
Patterning, compared with prior art, without expensive laboratory apparatus, micro-patterning is easily achieved, the addition of no nano antibacterial agent,
Material production cost is reduced, the pattern of titanium plate surface more easily regulates and controls.Meanwhile this method is with easy to operate, preparation
Rough surface, precision are high, wetability is controllable, unglazed photoresist and the advantages that developer pollution, while also can arbitrarily regulate and control surface
Micropatterning, a possibility that also eliminating antimicrobial nano particle and direct body contact and be enriched in vivo, more to human body
Add safe and reliable.
Summary of the invention
For overcome the deficiencies in the prior art, the object of the present invention is to provide to inhibit bacterial growth and promote protein
Titanium surface of adherency and preparation method thereof.
The purpose of the present invention is achieved through the following technical solutions.
To inhibit the preparation method on the titanium surface of bacterial growth and promotion protein adherence, comprising the following steps:
(1) titanium sheet being cut into fritter, is then cleaned, is degreased under ultrasonic state with cleaning solution, taking-up is dried, spare:
(2) electrolyte is prepared: ammonium fluoride is soluble in water, glycerine is then added, is uniformly mixed, obtains electrolyte:
(3) anodic oxidation: using platinized platinum as cathode, using the cleaned titanium sheet of step (1) as anode, the electrolyte obtained by step (2)
In be electrolysed:
(4) titanium sheet obtained by step (3) is placed in Muffle furnace and is annealed, obtain the TiO with photocatalytic activity2Film:
(5) by step (4), treated, and titanium sheet is soaked in hydrophobing agent, and sealing, which is protected from light, is placed on curing and drying in baking oven:
(6) by step (5), treated, and titanium sheet is placed under ultraviolet lamp, the mask made is placed in titanium sheet, illumination, is obtained
To inhibit bacterial growth and promote the titanium surface of protein adherence.
Preferably, in step (1), the specification of titanium sheet is 2cm × 3.5cm × 0.2mm after shearing, and the cleaning is to select clearly
Washing lotion is that acetone, ethyl alcohol, water respectively clean 15min according to this.
Preferably, the temperature of step (2) described stirring is 20-40 DEG C, time 15-30min.
Preferably, in step (3), electrolysis is carried out in 30-40 DEG C of water bath with thermostatic control, and voltage is with the speed of 0.1-0.3V/s
20-50V is risen to, 1-2h is electrolysed.
Preferably, in step (3), at a distance of 2-3cm between two electrodes when electrolysis.
Preferably, in step (4), the annealing is that Muffle furnace with the heating rate of 5-10 DEG C/min rises to 250-350 DEG C
Anneal 2-3h.
Preferably, in step (5), selected hydrophobing agent is that 17 fluorine certain herbaceous plants with big flowers ethyl triethoxy silicane alkane are dissolved in n-hexane
Mixed solution.
Preferably, in step (5), the temperature of the curing and drying is 100-140 DEG C, time 1-2h.
Preferably, in step (6), the time of the illumination is 3-5 minutes.
To inhibit bacterial growth and promote the titanium surface of protein adherence as made from above-described preparation method.
The present invention prepares a kind of micro- pattern nanostructure on the surface of titanium sheet, so that surface, which has, inhibits bacterial adhesion, from
And a kind of antibacterial film is formed on the surface of Titanium, while also can be conducive to protein adherence on the surface thereof.Micro-patterning
It is formed with photoetching process, sol-gel method, phase separation method and electrochemical method.The present invention carries out micro- figure using electrochemical method
The production of case is produced the topological structure that precision is 10nm-10um on the surface of titanium, is produced by electrochemical method micro-
Type structure mainly has netted and strip, makes it have the effect for inhibiting microorganism adhering and growth, while micro- pattern on surface
The surface area of titanium can effectively be increased by changing structure, provide more sites for the adherency of protein, this special titanium is mainly used
In in the manufacture of man-made organ, the problem of operative failure is led to due to bacterium infection is greatly reduced, itself and human body cell are promoted
Compatibility.It also can be applied on medical instrument simultaneously, instrument prevented to be infected by bacterial.
The realization of the invention is mainly a series of by the cleaning of titanium plate surface, anodic oxidation, annealing, hydrophobization, photoetching etc.
Step is realized, the titanium surface with micro-nano structure is prepared, which mainly includes reticular structure and strip structure, essence
Degree is 10nm-10um, the wetting of optical figure on surface such as Fig. 1 a(strip structure) and Fig. 4 (reticular structure) it is shown.It is connect by bacterium
Kind experiment discovery, the titanium sheet on the surface has preferable anti-microbial property, while passing through cell culture experiments, and discovery can promote significantly
Into the absorption of cell.
It is exactly that can greatly increase the energy of titanium surface anti-bacterial attachment in medical domain for usefulness of the present invention
Power reduces infection of the bacterium to wound in surgical procedure, reduces adherency of the bacterium to artificial skelecton tissue is moved into, promotes artifical bone
Bone is organizationally efficient and human body inner tissue cell adherency, selectivity improve reacting between osteocyte and immigration artifical bone, make
The integration obtained between bone and implantation material is promoted, to greatly improve the success rate of operation, while the titanium sheet energy on this surface
The growth of regulating cell is oriented, therefore can also be used to the growth of observation cell, development.
Compared with prior art, the present invention has the advantage that
1, titanium surface prepared by the present invention has the ability for inhibiting bacterial growth and promoting protein adherence.
2, compared with the existing technology, required equipment is simple by the present invention, easy to operate, substantially reduces cost.
3, the present invention can the hydrophobic position of flexible modulation surface hydrophilic and size, this for orient regulating cell growth
It is helpful.
Detailed description of the invention
Fig. 1 a is the top view with the moistened surface figure of striated structure.
Fig. 1 b be have the moistened surface figure of the micro- pattern of network top view (actual size of display area be 10
The mm of mm × 10).
Fig. 2 a is network exposure mask schematic diagram, and wherein p indicates adjacent lightproof area spacing, and i indicates hydrophobic mesh-like area
Width.
Fig. 2 b is network exposure mask exposure mask figure.
Fig. 3 a, Fig. 3 b are the TiO of Surface Creation2The SEM of nanotube schemes.
Fig. 4 is waffle-like pattern surface drop optics top view.
Fig. 5 is the side view of the moistened surface figure of the micro- pattern of network.
Fig. 6 is process flow chart of the invention.
Specific embodiment
Specific implementation of the invention is further described below in conjunction with example and attached drawing, but embodiments of the present invention
It is without being limited thereto.
Process flow chart of the invention is as shown in Figure 6.
The preparation of embodiment 1 has netted micropatterning
Before carrying out anodic oxidation to titanium sheet, by purity it is 99.9 %, shears to specification with a thickness of the bulk titanium sheet of 0.2 mm and be
2 cm×3.5 cm.Then Ti piece is successively cleaned by ultrasonic 15 min with acetone, ethyl alcohol, distilled water, degreases, takes out
It dries rear spare.
The electrolyte of anodic oxidation is by glycerine, H2O (20 vol%) and NH4F (1.0 wt%) composition first will
NH4F is dissolved in the water of 20 ml, and the glycerine of 80 ml is then added, and 20 min are stirred at 30 DEG C and are uniformly mixed.With
The platinized platinum of the cm of 2 cm × 3 is cathode, using the pure Ti piece of the cm of 2 cm × 3.5 of cleaning as anode, forms two electrode bodies
System carries out anodic oxidation, and two electrode spacings are 2 cm, and oxidation carries out in 30 DEG C of waters bath with thermostatic control.It will be electric when anodic oxidation starts
Pressure is with 0.2Vs-1Speed slowly and steadily rise to 35 V, keep voltage 1.5h.
The unformed TiO that anodic oxidation obtains2 Layer does not have photocatalytic activity.Therefore, it is necessary to by unformed TiO2 By
Annealing Crystallization forms the Anatase TiO with photocatalytic activity2.There to be TiO2The titanium sheet of film is placed in chamber type electric resistance furnace
In anneal, heating rate be 7.5 DEG C of min-1, anneal 1.5h at 300 DEG C, then natural cooling.Had at this time
There is photocatalytic activity TiO2The titanium sheet of film.
0.1mlFAS-17 is dissolved in 12ml n-hexane, the TiO containing anatase after taking annealing2The titanium sheet of film is soaked in
In solution, sealing is protected from light 2h.Titanium sheet is then placed in solidification in 120 DEG C of baking oven, dry 1.5 h.
It opens ultraviolet lamp to cover netted mask in Ti on piece after light stability, and is exposed to UV intensity and is
20mw/cm2Ultraviolet lamp under 3min by photoetching just obtain the patterned surface with mixed wettability, the pattern on surface
Change the resolution ratio that resolution ratio depends on mask, this experiment is prepared patterned having a size of 3um, the design drawing of mask such as attached drawing 2a
With shown in 2b, Fig. 2 a is mask hydrophobe dimensional drawing, and i is the distance at hydrophobic interval, and p is the distance between two hydrophobic lattices.
Naked eyes can not tell the pattern on the surface, but display after being soaked with water, (the vertical view after wetting as shown in Figure 1 b
Figure).
The preparation of embodiment 2 has netted micropatterning
First be 99.9 % by purity, with a thickness of the bulk titanium sheet of 0.2 mm, to shear to specification be the cm of 2 cm × 3.5.According to
It is secondary that Ti piece is cleaned by ultrasonic 15 min with acetone, ethyl alcohol, distilled water, it degreases, taking-up is dried rear spare.
The electrolyte of anodic oxidation is by glycerine, H2O (20 vol%) and NH4F (1.0 wt%) composition first will
NH4F is dissolved in the water of 20 ml, and the glycerine of 80 ml is then added, and 20 min are stirred at 30 DEG C and are uniformly mixed.With
The platinized platinum of the cm of 2 cm × 3 is cathode, and using the pure Ti piece of the cm of 2 cm × 3.5 of cleaning as anode, two electrode spacings are
3 cm are carried out in 40 DEG C of waters bath with thermostatic control.By voltage with 0.3Vs-1Speed slowly and steadily rise to 50V, keep voltage
1.5h。
There to be TiO2The titanium sheet of film, which is placed in chamber type electric resistance furnace, anneals, and heating rate is 10 DEG C of min-1,
Anneal 1.5h at 350 DEG C, then natural cooling.It obtains with photocatalytic activity TiO2The titanium sheet of film.
0.1mlFAS-17 is dissolved in 12ml n-hexane, the TiO containing anatase after taking annealing2The titanium sheet of film is soaked in
In solution, sealing is protected from light 4h.Titanium sheet is then placed in solidification in 140 DEG C of baking oven, dry 2h.
It opens ultraviolet lamp to cover netted mask in Ti on piece after light stability, and is exposed to UV intensity and is
20mw/ cm2Ultraviolet lamp under 3 min by photoetching just obtain the patterned surface with mixed wettability, the figure on surface
Case resolution ratio depends on the resolution ratio of mask, this experiment is prepared patterned having a size of 3um, the design drawing of mask such as attached drawing
Shown in 2a and 2b, Fig. 2 a be mask hydrophobe dimensional drawing, i be hydrophobic interval distance, p between two hydrophobic lattices away from
From.Naked eyes can not tell the pattern on the surface, but display after being soaked with water, as shown in Figure 4.Fig. 5 is after soaking
Side view.
The preparation of embodiment 3 has the micropatterning of strip
First be 99.9 % by purity, with a thickness of the bulk titanium sheet of 0.2 mm, to shear to specification be the cm of 2 cm × 3.5.According to
It is secondary that Ti piece is cleaned by ultrasonic 15 min with acetone, ethyl alcohol, distilled water, it degreases, taking-up is dried rear spare.
The electrolyte of anodic oxidation is by glycerine, H2O (20 vol%) and NH4F (1.0 wt%) composition first will
NH4F is dissolved in the water of 20 ml, and the glycerine of 80 ml is then added, and 30 min are stirred at 40 DEG C and are uniformly mixed.With
The platinized platinum of the cm of 2 cm × 3 is cathode, and using the pure Ti piece of the cm of 2 cm × 3.5 of cleaning as anode, two electrode spacings are
2cm is carried out in 40 DEG C of waters bath with thermostatic control.By voltage with 0.1Vs-1Speed slowly and steadily rise to 20 V, keep voltage
1h。
There to be TiO2The titanium sheet of film, which is placed in chamber type electric resistance furnace, anneals, and heating rate is 5 DEG C of min-1,
Anneal 1h at 250 DEG C, then natural cooling.It obtains with photocatalytic activity TiO2The titanium sheet of film.
0.1mlFAS-17 is dissolved in 12ml n-hexane, the TiO containing anatase after taking annealing2The titanium sheet of film is soaked in
In solution, sealing is protected from light 1h.Titanium sheet is then placed in solidification in 100 DEG C of baking oven, dry 2h.
It opens ultraviolet lamp to cover netted mask in Ti on piece after light stability, and is exposed to UV intensity and is
20mw/ cm2Ultraviolet lamp under 3 min by photoetching just obtain the patterned surface with mixed wettability, the figure on surface
Case resolution ratio depends on the resolution ratio of mask, and the precision of strip micropatterning is 4um, it can not visually tell the surface
Pattern, but displayed after being soaked with water, as shown in Figure 1a (optics picture size is 10mm x 10mm).
The titanium sheet after anodic oxidation is observed by field emission scanning electron microscope, obtains surface texture such as Fig. 3 a
The side view of (top view of SEM) and 3b(SEM) shown in, nanotube-shaped two of Surface Creation as can clearly see from the figure
Titanium oxide structure.
Claims (10)
1. the preparation method on the titanium surface to inhibit bacterial growth and promotion protein adherence, which is characterized in that including following
Step:
(1) titanium sheet being cut into fritter, is then cleaned under ultrasonic state with cleaning solution, taking-up is dried, spare:
(2) electrolyte is prepared: ammonium fluoride is soluble in water, glycerine is then added, is uniformly mixed, obtains electrolyte:
(3) using platinized platinum as cathode, using the cleaned titanium sheet of step (1) as anode, electrolysis obtained by step (2) anodic oxidation: is added
Liquid is electrolysed:
(4) titanium sheet obtained by step (3) is placed in Muffle furnace and is annealed, obtain the TiO with photocatalytic activity2Film:
(5) by step (4), treated, and titanium sheet is soaked in hydrophobing agent, and sealing, which is protected from light, is placed on curing and drying in baking oven:
(6) by step (5), treated, and titanium sheet is placed under ultraviolet lamp, the mask made is placed in titanium sheet, illumination, is obtained
To inhibit bacterial growth and promote the titanium surface of protein adherence.
2. preparation method according to claim 1, which is characterized in that in step (1), the specification of titanium sheet is 2cm after shearing
× 3.5cm × 0.2mm, the cleaning solution are that acetone, ethyl alcohol, water respectively clean 15min according to this.
3. preparation method according to claim 1, which is characterized in that the temperature of step (2) described stirring is 20-40 DEG C,
Time is 15-30min.
4. preparation method according to claim 1, which is characterized in that in step (3), electrolysis is in 30-40 DEG C of thermostatted water
It is carried out in bath, voltage rises to 20-50V with the speed of 0.1V/s-0.3V/s, is electrolysed 1-2h.
5. preparation method according to claim 1, which is characterized in that in step (3), at a distance of 2- between two electrodes when electrolysis
3cm。
6. preparation method according to claim 1, which is characterized in that in step (4), the annealing is Muffle furnace with 5-10
DEG C/heating rate of min rises to 250-350 DEG C of annealing 2-3h.
7. preparation method according to claim 1, which is characterized in that in step (5), selected hydrophobing agent is by 17
Fluorine certain herbaceous plants with big flowers ethyl triethoxy silicane alkane is dissolved in the mixed solution of n-hexane.
8. preparation method according to claim 1, which is characterized in that in step (5), the temperature of the curing and drying is
100-140 DEG C, time 1-2h.
9. preparation method according to claim 1, which is characterized in that in step (6), the time of the illumination is 3-5 points
Clock.
10. to inhibit bacterial growth and protein is promoted to glue as made from the described in any item preparation methods of claim 1-9
Attached titanium surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810951824.5A CN109125809A (en) | 2018-08-21 | 2018-08-21 | To inhibit bacterial growth and promote the titanium surface and preparation method thereof of protein adherence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810951824.5A CN109125809A (en) | 2018-08-21 | 2018-08-21 | To inhibit bacterial growth and promote the titanium surface and preparation method thereof of protein adherence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109125809A true CN109125809A (en) | 2019-01-04 |
Family
ID=64790320
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810951824.5A Pending CN109125809A (en) | 2018-08-21 | 2018-08-21 | To inhibit bacterial growth and promote the titanium surface and preparation method thereof of protein adherence |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109125809A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113730042A (en) * | 2021-09-09 | 2021-12-03 | 北京理工大学 | Orthopedic implant with multistage micro-nano structure and manufacturing method thereof |
| CN110436407B (en) * | 2019-07-26 | 2022-02-11 | 广州大学 | Preparation method and application of efficient antibacterial synergistic anti-bacterial adhesion nano material |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1226918A (en) * | 1996-05-31 | 1999-08-25 | 东陶机器株式会社 | Antifouling member and coating composition |
| US20040259374A1 (en) * | 2003-06-19 | 2004-12-23 | Nec Electronics Corporation | Semiconductor device and method for manufacturing same |
| CN100998892A (en) * | 2006-12-31 | 2007-07-18 | 厦门大学 | Preparation method of nanometer ordered structure biomaterial membranous layer based on super hydro philic/hydro phobic characteristic template |
| CN102345134A (en) * | 2011-09-13 | 2012-02-08 | 蔺增 | Preparation method for wettability controllable porous structure of titanium and titanium alloy surface |
| CN106902384A (en) * | 2017-03-07 | 2017-06-30 | 珠海乔丹科技股份有限公司 | A kind of method for preparing class bone structure film layer on titanium surface |
| CN107324276A (en) * | 2017-06-22 | 2017-11-07 | 华南理工大学 | A kind of micro-patterning wettable surfaces and preparation method and application are in spray cooling device |
| CN107983601A (en) * | 2017-11-17 | 2018-05-04 | 华南理工大学 | A kind of micro-patterning wettable surfaces and its preparation method and application |
| BR202018004368U2 (en) * | 2018-03-05 | 2019-09-17 | Bruno Dos Santos Pavei | IMPROVEMENT INTRODUCED IN DENTAL IMPLANT |
-
2018
- 2018-08-21 CN CN201810951824.5A patent/CN109125809A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1226918A (en) * | 1996-05-31 | 1999-08-25 | 东陶机器株式会社 | Antifouling member and coating composition |
| US20040259374A1 (en) * | 2003-06-19 | 2004-12-23 | Nec Electronics Corporation | Semiconductor device and method for manufacturing same |
| CN100998892A (en) * | 2006-12-31 | 2007-07-18 | 厦门大学 | Preparation method of nanometer ordered structure biomaterial membranous layer based on super hydro philic/hydro phobic characteristic template |
| CN102345134A (en) * | 2011-09-13 | 2012-02-08 | 蔺增 | Preparation method for wettability controllable porous structure of titanium and titanium alloy surface |
| CN106902384A (en) * | 2017-03-07 | 2017-06-30 | 珠海乔丹科技股份有限公司 | A kind of method for preparing class bone structure film layer on titanium surface |
| CN107324276A (en) * | 2017-06-22 | 2017-11-07 | 华南理工大学 | A kind of micro-patterning wettable surfaces and preparation method and application are in spray cooling device |
| CN107983601A (en) * | 2017-11-17 | 2018-05-04 | 华南理工大学 | A kind of micro-patterning wettable surfaces and its preparation method and application |
| BR202018004368U2 (en) * | 2018-03-05 | 2019-09-17 | Bruno Dos Santos Pavei | IMPROVEMENT INTRODUCED IN DENTAL IMPLANT |
Non-Patent Citations (1)
| Title |
|---|
| 沈一州等: "《分级结构表面的超疏水特性与应用》", 28 February 2017, 西北工业大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110436407B (en) * | 2019-07-26 | 2022-02-11 | 广州大学 | Preparation method and application of efficient antibacterial synergistic anti-bacterial adhesion nano material |
| CN113730042A (en) * | 2021-09-09 | 2021-12-03 | 北京理工大学 | Orthopedic implant with multistage micro-nano structure and manufacturing method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xin et al. | Bioactive SrTiO3 nanotube arrays: strontium delivery platform on Ti-based osteoporotic bone implants | |
| Dong et al. | Multifunctional nanowire bioscaffolds on titanium | |
| Skorb et al. | Surface Nanoarchitecture for Bio‐Applications: Self‐Regulating Intelligent Interfaces | |
| CN102677125B (en) | Preparation method of active antibacterial composite coating on surface of titanium and titanium alloy medical instrument | |
| JP4199545B2 (en) | Method for improving soft tissue adhesion and use of said method for graft preparation | |
| CN107096068A (en) | A kind of preparation method of dentistry implant and its bioactivity antimicrobial surface | |
| Ge et al. | Cicada-inspired fluoridated hydroxyapatite nanostructured surfaces synthesized by electrochemical additive manufacturing | |
| CN103110981B (en) | Method for preparing antibacterial active titanium oxide nanotube array composite coating material | |
| CN101570874B (en) | In situ formation method of gradient film containing TiO*/HA/CaCO* | |
| IT9020513A1 (en) | BIOCOMPATIBLE PERFORATED MEMBRANES, PROCESSES FOR THEIR PREPARATION, THEIR USE AS A SUPPORT FOR THE IN VITRO GROWTH OF EPITHELIAL CELLS, ARTIFICIAL LEATHER THUS OBTAINED AND THEIR USE IN LEATHER TRANSPLANTS | |
| Maher et al. | Advancing of additive-manufactured titanium implants with bioinspired micro-to nanotopographies | |
| CN109125809A (en) | To inhibit bacterial growth and promote the titanium surface and preparation method thereof of protein adherence | |
| CN105177670A (en) | Method for growing nanotubes on surface of titanium-based material of three-dimensional porous structure in in-situ manner | |
| CN108144111A (en) | A kind of planting body active surface | |
| CN108042846B (en) | Preparation method of strontium-doped tantalum oxide nanorod structure bioactive coating | |
| Wu et al. | Nano-hydroxyapatite coated TiO2 nanotubes on Ti-19Zr-10Nb-1Fe alloy promotes osteogenesis in vitro | |
| CN106562827A (en) | Hydrophilic and antibacterial dental implant system and manufacturing method thereof | |
| Qian et al. | Selective biofunctional modification of titanium implants for osteogenic and antibacterial applications | |
| CN113529158A (en) | Process for preparing porous structure on surface of TC4 titanium alloy by electrochemical dealloying method | |
| CN108144125A (en) | A kind of planting body with surface active coating | |
| Jiang et al. | Recent advances in light-induced cell sheet technology | |
| AU2021105727A4 (en) | A method of preparation of Silk Fibroins coated with Hybrid chitosan-ZnO nanoparticles for wound dressing. | |
| CN108498859B (en) | Antibacterial bioactive glass nanofiber scaffold and preparation method thereof | |
| CN101565824A (en) | Ultraviolet light irradiation method for increasing surface biological activity of titanium or alloy thereof | |
| CN101491692A (en) | Preparation method of calcium titanate nano-tube array biological coatings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190104 |