CN109125785A - It is a kind of for anti-inflammatory medicament-carrying nano-fiber membrane preparation method - Google Patents
It is a kind of for anti-inflammatory medicament-carrying nano-fiber membrane preparation method Download PDFInfo
- Publication number
- CN109125785A CN109125785A CN201810809688.6A CN201810809688A CN109125785A CN 109125785 A CN109125785 A CN 109125785A CN 201810809688 A CN201810809688 A CN 201810809688A CN 109125785 A CN109125785 A CN 109125785A
- Authority
- CN
- China
- Prior art keywords
- solution
- nano
- fibrous membrane
- nano fibrous
- spinning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/24—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/216—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with other specific functional groups, e.g. aldehydes, ketones, phenols, quaternary phosphonium groups
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/41—Anti-inflammatory agents, e.g. NSAIDs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Textile Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Preparation (AREA)
- Artificial Filaments (AREA)
Abstract
The invention discloses a kind of for anti-inflammatory medicament-carrying nano-fiber membrane preparation method.This method is prepared with the nano fibrous membrane of slow releasing function, good biocompatibility by electrostatic spinning technique, so that the raising of nano fibrous membrane crystallinity is delayed its swelling by the method for modifying of safety.The drug being supported is 5 hydroxymethyl furfural.The characteristics of medicament-carrying nano-fiber membrane is that have good biocompatibility with slow releasing function and with skin and human body in vivo, simultaneously drug loading and nano fibrous membrane, 5 hydroxymethyl furfural can be made gradually to discharge, reach single administration, anti-inflammatory effect can be played at longer time, nanofiber diameter of the invention is uniform, can effectively prevent the intrusion of bacterium.
Description
Technical field
The invention belongs to technical field of nano material.It is related to a kind of for anti-inflammatory medicament-carrying nano-fiber membrane preparation method.
Background technique
5 hydroxymethyl furfural is the product of common heterocycle Maillard reaction, and the main acid catalysis heat dehydration by fructose is raw
It produces and is used as the flavoring substance in heat treated article.It has many excellent pharmacological actions, such as anti-inflammatory, antioxidant activity, prevents
Only acute hypoxia inhibits oxidative damage and cardioprotection of alcoholic liver etc..
Electrostatic spinning is that technology is a kind of (mainly to be polymerize for making super thin fiber of the diameter from several microns to tens nanometer
Object) simple and highly versatile method.During a typical electrostatic spinning, three parts: high-voltage electricity are generally required
Source, capillary and typically grounded collector with spinning head.In most cases, spinning head connection is that supply is poly-
The syringe of polymer solution, solution can be used syringe pump and supplied with constant rate by spinning head.When a high voltage is applied,
Polymer solution hanging drop at spinnerette orfice becomes electrostatic charge, and charge inducing can be uniformly distributed on the surface simultaneously.The table of drop
Face tension normally results in sphere and is in equilibrium state, and will lead to the end that protrusion appears in drop when the accumulation of charge
On, make drop deformation at the conical by its shape of taylor cone.With the increase of voltage/field strength, repels electrostatic force and overcome surface tension, when
When reaching critical value, electrified jet can be sprayed from the top of taylor cone, as solvent evaporates, will form diameter from several microns to several
The solid polymerization fibres of nanometer, the easily prepared uniform composite material of electrostatic spinning technique.
Polyvinyl alcohol (PVA) is used as a kind of artificial synthesized high molecular material, is by polyvinyl acetate through alkali catalyzed alcoholysis
And obtain, it is convenient, cheap, safe to the human body to synthesize, and has good biocompatibility, has preferable slow-release function, is
A kind of excellent pharmaceutic adjuvant of great potential, meanwhile, film forming is preferable.But simultaneously, polyvinyl alcohol also has its deficiency
Place can pass through addition natural macromolecular material if dehydration rate and swellbility are not able to satisfy the requirement of particular stent material thus
It improves its performance.
Chitosan (CS) is a kind of natural macromolecular material, as the unique alkaline polysaccharide of nature, is had degradable, anti-
The various biologicals performances such as bacterium is antibacterial, good biocompatibility, hemostasis.Although chitosan there are many advantages, pass through
It is difficult only to carry out spinning with chitosan, it is blended with other materials thus, its advantage can be given full play to, and wanting for spinning can be reached
It asks.
Therefore, advantage blended by both PVA and chitosan, both can make full use of, at the same make up it is respective not
Foot place, while the drug 5 hydroxymethyl furfural with anti-inflammatory effect is loaded into nano fibrous membrane, long-acting it can play anti-inflammatory work
With this nano fibrous membrane can be used as wound dressing or treatment disease relevant to inflammation.
Summary of the invention
The purpose of the present invention is to provide a kind of improvement swelling ratio, the preparation sides of the nano fibrous membrane with slow releasing function
Method.
Technical scheme is as follows:
It is a kind of for anti-inflammatory medicament-carrying nano-fiber membrane preparation method, include the following steps:
(1) polyvinyl alcohol (PVA) of mass percent concentration 6-10% is prepared with deionized water, be 60- with concentration of volume percent
90% acetum prepares chitosan (CS) Polymer Solution of mass percent concentration 1-3 %, then by chitosan solution with
Poly-vinyl alcohol solution is mixed according to the ratio of volume ratio 1:4-6, is stayed overnight using magnetic stirrer, is obtained uniformly yellowish
Color clear solution；
(2) anti-inflammatory drug 5 hydroxymethyl furfural (5-HMF) is added in light yellow transparent solution makes its concentration 50-150 μ g/
Solution is carried out ultrasound by mL, so that drug is well-dispersed in Polymer Solution and is then continued to carry out magnetic agitation, obtains homogeneous transparent
Electrostatic spinning precursor solution；
(3) precursor solution obtained by step (2) is transferred in electrostatic spinning injector for medical purpose, adjustment spinning receives distance and is
15-18 cm, spinning voltage are 18-20 kv, flow rate set is 0.2-0.6 mL/h, and the spinning on electrostatic spinning apparatus uses
Aluminium foil receives nano fibrous membrane；
(4) nano fibrous membrane that step (3) obtains is put to normal-temperature vacuum drying box and is dried, and is using concentration at 50-80 DEG C
The glutaraldehyde water solution of 2-5 % is placed in generation steam in the glass container device of sealing and is crosslinked to nano fibrous membrane, so
It dries in a vacuum drying oven afterwards, obtains the higher nano fibrous membrane of crystallinity.
Further, in step (2), the time of the ultrasound is 120-180 min.
Further, in step (4), the condition of the drying are as follows: 2-3 h is dried at 50-60 DEG C.
The drug that nano fibrous membrane of the present invention is loaded into has anti-inflammatory effect, and polyvinyl alcohol and chitosan have good biology
Compatibility, nano fibrous membrane have slow releasing function to drug.
Compared with prior art, the present invention has the following technical effect that:
Preparation process of the present invention is simple, and obtained nano fibrous membrane has good biocompatibility, is loaded with the medicine of antiphlogistic effects
Object, so that drug is able to slow release；By being crosslinked, so that the use scope of nano fibrous membrane is wider.Load medicine of the invention is received
Rice tunica fibrosa uniform diameter, can stop the invasion of bacterium, can be used as a kind of ideal wound dressing.
Detailed description of the invention
Fig. 1 is the scanning electron microscope (SEM) photograph of the load medicine PVA/ chitosan nano fiber membrane of embodiment one of the present invention preparation.
Fig. 2 is the scanning electron microscope (SEM) photograph (amplification of the load medicine PVA/ chitosan nano fiber membrane of embodiment one of the present invention preparation
30000 times of multiple).
Fig. 3 is the survey of the load medicine PVA/ chitosan nano fiber membrane cell biological compatibility of embodiment one of the present invention preparation
Test result.
Fig. 4 is the expression effect of the load medicine PVA/ chitosan nano fiber membrane anti-inflammatory factors of embodiment one of the present invention preparation
Fruit.
Fig. 5 is the expression effect of the load medicine PVA/ chitosan nano fiber membrane anti-inflammatory factors of embodiment one of the present invention preparation
Act on duration.
Fig. 6 is that dissolution/degradation situation of the load medicine PVA/ chitosan nano fiber membrane of embodiment one of the present invention preparation is examined
It examines.
Specific embodiment
The present invention will be further specifically described in detail with reference to specific embodiments, but embodiments of the present invention are not
It is limited to this, for not specifically specified technological parameter, can refer to routine techniques progress.
Embodiment 1
1, it weighs 8 g polyvinyl alcohol to be dissolved in 100 mL distilled water, heating promotes dissolution at 80 DEG C, and it is molten to weigh 2 g chitosans
In 100 mL, 90 % glacial acetic acid solution, heating stirring dissolves at 60 DEG C；Both uniform with magnetic stirrer.
2, the poly-vinyl alcohol solution stirred evenly and chitosan solution are mixed according to the ratio of volume ratio 4:1,
12 h of magnetic stirrer, obtains uniform light yellow transparent solution.
3, it is the 5 hydroxymethyl furfural (5-HMF) of 100 mg/mL in polyvinyl alcohol/shell of 10 ml that 10 μ L concentration, which are added,
The uniformly mixed solution of glycan, makes its final concentration of 100 μ g/mL, and solution is carried out 120 min of ultrasound, keeps drug fully dispersed
It then continues to carry out magnetic agitation in Polymer Solution, obtains the electrostatic spinning precursor solution of homogeneous transparent.
4, spinning solution is transferred in electrostatic spinning injector for medical purpose, and adjustment spinning distance is 15 cm, spinning voltage 20
Kv, flow rate set are 0.6 mL/h, and spinning is carried out on electrostatic spinning apparatus, receive nano fibrous membrane using aluminium foil.
5, nano fibrous membrane at 50 DEG C with after 24 h of glutaraldehyde vapor crosslinking of 2 %, the vacuum drying 2 at 50 DEG C
H, the nano fibrous membrane after being crosslinked.
Fig. 1 and Fig. 2 is the scanning electron microscopic picture of nanofiber, and amplification factor is 10000 times and 30000 times, can from figure
To find out that nano fibrous membrane diameter is evenly distributed, spinning solution has obtained sufficient stretching, and average fibre diameter is 200 nm left
The right side can effectively inhibit the entrance of bacterium.
The creep plate for being loaded with nanofiber is placed in 12 orifice plates, by macrophage, skin at fibre after ultraviolet light irradiates
Cell is tieed up, horn cell culture is cultivated 24 h on nano fibrous membrane, then tested with CCK8 kit.
The creep plate for being loaded with nano fibrous membrane is placed in 12 orifice plates, is being received macrophage culture after ultraviolet light irradiates
On rice tunica fibrosa, then giving lipopolysaccharides (LPS) stimulates 12 h, with the expression of nitric oxide kit test NO and not
With the operative condition of time.It can from Fig. 4 (medicament-carrying nano-fiber membrane influences the macrophage NO of LPS induction RAW264.7)
Medicament-carrying nano-fiber membrane can be effectively reduced the expression of inflammatory factor out, and explanation can effectively inhibit inflammation；It (is carried from Fig. 5 simultaneously
Medicine nano fibrous membrane influences the macrophage NO of LPS induction RAW264.7) it is known that nano fibrous membrane has certain sustained release
Effect, until 12 h, nano fibrous membrane can also play antiphlogistic effects.
The study on the stability of nano fibrous membrane: taking out nano fibrous membrane in vacuum oven, weighs its quality and records,
Then nano fibrous membrane is impregnated in deionized water, in particular point in time, (3h, 6 h and 12 h) take out and be placed on vacuum drying
Case drying, then weighs its quality, and each time point carries out 3 retests.From fig. 6, it can be seen that the Nanowire after impregnating
Point shows after glutaraldehyde cross-linking, the non-fusibility energy of nano fibrous membrane dimension film there is no significantly degrading in different times
It increases.
Embodiment 2
1, it weighs 10 g polyvinyl alcohol to be dissolved in 100 mL distilled water, heating promotes dissolution at 80 DEG C, and it is poly- to weigh 1.5 g shells
Sugar is dissolved in 100 mL, 90 % glacial acetic acid solution, and heating stirring dissolves at 60 DEG C；Both equal with magnetic stirrer
It is even.
2, the poly-vinyl alcohol solution stirred evenly and chitosan solution are mixed according to the ratio of volume ratio 5:1,
12 h of magnetic stirrer, obtains uniform light yellow transparent solution.
3, it is the 5 hydroxymethyl furfural (5-HMF) of 100 mg/mL in polyvinyl alcohol/shell of 10 ml that 10 μ L concentration, which are added,
The uniformly mixed solution of glycan, makes its final concentration of 100 μ g/mL, and solution is carried out 120 min of ultrasound, keeps drug fully dispersed
It then continues to carry out magnetic agitation in Polymer Solution, obtains the electrostatic spinning precursor solution of homogeneous transparent.
4, spinning solution is transferred in electrostatic spinning injector for medical purpose, and adjustment spinning distance is 20 cm, spinning voltage 25
Kv, flow rate set are 0.6 mL/h, and spinning is carried out on electrostatic spinning apparatus, receive nano fibrous membrane using aluminium foil.
5, nano fibrous membrane at 50 DEG C with after 24 h of glutaraldehyde vapor crosslinking of 3 %, the vacuum drying 2 at 60 DEG C
H, the nano fibrous membrane after being crosslinked.
Embodiment 3
1, it weighs 8 g polyvinyl alcohol to be dissolved in 100 mL distilled water, heating promotes dissolution at 80 DEG C, and it is molten to weigh 2 g chitosans
In 100 mL, 90 % glacial acetic acid solution, heating stirring dissolves at 60 DEG C；Both uniform with magnetic stirrer.
2, the poly-vinyl alcohol solution stirred evenly and chitosan solution are mixed according to the ratio of volume ratio 5:1,
12 h of magnetic stirrer, obtains uniform light yellow transparent solution.
3, it is the 5 hydroxymethyl furfural (5-HMF) of 100 mg/mL in polyvinyl alcohol/shell of 10 ml that 10 μ L concentration, which are added,
The uniformly mixed solution of glycan, makes its final concentration of 100 μ g/mL, and solution is carried out 120 min of ultrasound, keeps drug fully dispersed
It then continues to carry out magnetic agitation in Polymer Solution, obtains the electrostatic spinning precursor solution of homogeneous transparent.
4, spinning solution is transferred in electrostatic spinning injector for medical purpose, and adjustment spinning distance is 15 cm, spinning voltage 20
Kv, flow rate set are 0.4 mL/h, and spinning is carried out on electrostatic spinning apparatus, receive nano fibrous membrane using aluminium foil.
5, nano fibrous membrane at 50 DEG C with after 24 h of glutaraldehyde vapor crosslinking of 2 %, the vacuum drying 2 at 50 DEG C
H, the nano fibrous membrane after being crosslinked.
The above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be to the present invention
Embodiment restriction.For those of ordinary skill in the art, it can also make on the basis of the above description
Other various forms of variations or variation.There is no necessity and possibility to exhaust all the enbodiments.It is all of the invention
Made any modifications, equivalent replacements, and improvements etc., should be included in the protection of the claims in the present invention within spirit and principle
Within the scope of.
Claims (3)
1. a kind of for anti-inflammatory medicament-carrying nano-fiber membrane preparation method, which comprises the steps of:
(1) polyvinyl alcohol (PVA) of mass percent concentration 6-10% is prepared with deionized water, be 60- with concentration of volume percent
90% acetum prepares chitosan (CS) Polymer Solution of mass percent concentration 1-3 %, then by chitosan solution with
Poly-vinyl alcohol solution is mixed according to the ratio of volume ratio 1:4-6, is stayed overnight using magnetic stirrer, is obtained uniformly yellowish
Color clear solution；
(2) anti-inflammatory drug 5 hydroxymethyl furfural (5-HMF) is added in light yellow transparent solution makes its concentration 50-150 μ g/
Solution is carried out ultrasound by mL, so that drug is well-dispersed in Polymer Solution and is then continued to carry out magnetic agitation, obtains homogeneous transparent
Electrostatic spinning precursor solution；
(3) precursor solution obtained by step (2) is transferred in electrostatic spinning injector for medical purpose, adjustment spinning receives distance and is
15-18 cm, spinning voltage are 18-25 kv, flow rate set is 0.2-0.6 mL/h, and the spinning on electrostatic spinning apparatus uses
Aluminium foil receives nano fibrous membrane；
(4) nano fibrous membrane that step (3) obtains is put to normal-temperature vacuum drying box and is dried, and is using concentration at 50-80 DEG C
The glutaraldehyde water solution of 2-5 % is placed in generation steam in the glass container device of sealing and is crosslinked to nano fibrous membrane, so
It dries in a vacuum drying oven afterwards, obtains the higher nano fibrous membrane of crystallinity.
2. being used for anti-inflammatory medicament-carrying nano-fiber membrane preparation method according to claim 1, which is characterized in that in step (2),
The time of the ultrasound is 120-180 min.
3. being used for anti-inflammatory medicament-carrying nano-fiber membrane preparation method according to claim 1, which is characterized in that in step (4),
The condition of the drying are as follows: 2-3 h is dried at 50-60 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810809688.6A CN109125785A (en) | 2018-07-23 | 2018-07-23 | It is a kind of for anti-inflammatory medicament-carrying nano-fiber membrane preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810809688.6A CN109125785A (en) | 2018-07-23 | 2018-07-23 | It is a kind of for anti-inflammatory medicament-carrying nano-fiber membrane preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109125785A true CN109125785A (en) | 2019-01-04 |
Family
ID=64801236
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810809688.6A Pending CN109125785A (en) | 2018-07-23 | 2018-07-23 | It is a kind of for anti-inflammatory medicament-carrying nano-fiber membrane preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109125785A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109881369A (en) * | 2019-02-27 | 2019-06-14 | 浙江工业大学 | A kind of preparation method for reinforcing antibacterial vapor-permeable type composite fibre static spinning membrane |
| CN110152049A (en) * | 2019-06-10 | 2019-08-23 | 东北林业大学 | A kind of preparation method for the chitosan-based medicament-carrying nano-fiber membrane can be applied to medical wound dressing |
| CN112047478A (en) * | 2020-08-10 | 2020-12-08 | 中国科学院城市环境研究所 | Method for removing nitrate from microalgae composite material fixed by electrostatic spinning fiber membrane |
| CN114073786A (en) * | 2020-08-13 | 2022-02-22 | 中国人民解放军总医院 | Liquid-absorbing antibacterial 3D nanofiber composite medical dressing and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013096605A1 (en) * | 2011-12-20 | 2013-06-27 | Bionova Medical, Inc. | Systems and methods for delivering an agent to a wound under negative pressure |
| CN105457081A (en) * | 2015-12-14 | 2016-04-06 | 华南理工大学 | Water-dissolved abandoned broad-spectrum non-toxic dressing and preparation method thereof |
| CN107334749A (en) * | 2017-07-03 | 2017-11-10 | 苏州大学 | A kind of preparation method for the polyvinyl alcohol chitosan nano fiber for carrying captopril |
-
2018
- 2018-07-23 CN CN201810809688.6A patent/CN109125785A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013096605A1 (en) * | 2011-12-20 | 2013-06-27 | Bionova Medical, Inc. | Systems and methods for delivering an agent to a wound under negative pressure |
| CN105457081A (en) * | 2015-12-14 | 2016-04-06 | 华南理工大学 | Water-dissolved abandoned broad-spectrum non-toxic dressing and preparation method thereof |
| CN107334749A (en) * | 2017-07-03 | 2017-11-10 | 苏州大学 | A kind of preparation method for the polyvinyl alcohol chitosan nano fiber for carrying captopril |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109881369A (en) * | 2019-02-27 | 2019-06-14 | 浙江工业大学 | A kind of preparation method for reinforcing antibacterial vapor-permeable type composite fibre static spinning membrane |
| CN110152049A (en) * | 2019-06-10 | 2019-08-23 | 东北林业大学 | A kind of preparation method for the chitosan-based medicament-carrying nano-fiber membrane can be applied to medical wound dressing |
| CN110152049B (en) * | 2019-06-10 | 2021-12-07 | 东北林业大学 | Application of chitosan-based nanofiber membrane of medical wound dressing |
| CN112047478A (en) * | 2020-08-10 | 2020-12-08 | 中国科学院城市环境研究所 | Method for removing nitrate from microalgae composite material fixed by electrostatic spinning fiber membrane |
| CN114073786A (en) * | 2020-08-13 | 2022-02-22 | 中国人民解放军总医院 | Liquid-absorbing antibacterial 3D nanofiber composite medical dressing and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109125785A (en) | It is a kind of for anti-inflammatory medicament-carrying nano-fiber membrane preparation method | |
| CN103611182B (en) | Preparation method of core-shell structure superfine fiber carrier material for medical dressing | |
| Akrami-Hasan-Kohal et al. | Curcumin-loaded naturally-based nanofibers as active wound dressing mats: Morphology, drug release, cell proliferation, and cell adhesion studies | |
| Heydari et al. | Preparation and evaluation of poly glycerol sebacate/poly hydroxy butyrate core‐shell electrospun nanofibers with sequentially release of ciprofloxacin and simvastatin in wound dressings | |
| Liu et al. | Regenerated cellulose micro-nano fiber matrices for transdermal drug release | |
| Duan et al. | A nanofibrous composite membrane of PLGA–chitosan/PVA prepared by electrospinning | |
| Fu et al. | Acceleration of dermal wound healing by using electrospun curcumin‐loaded poly (ε‐caprolactone)‐poly (ethylene glycol)‐poly (ε‐caprolactone) fibrous mats | |
| Kalani et al. | Electrospun core-sheath poly (vinyl alcohol)/silk fibroin nanofibers with Rosuvastatin release functionality for enhancing osteogenesis of human adipose-derived stem cells | |
| Tanha et al. | G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo | |
| CN106110371A (en) | A kind of preparation method of high-hydroscopicity sodium alginate composite nano fiber wound dressing | |
| Khan | Applications of electrospun nanofibers in the biomedical field | |
| CN102178640A (en) | Method for loading hydrophobic medicament uniformly on hydrophilic polymer electrospinning nanofiber | |
| Nada et al. | Encapsulation of nicotinamide into cellulose based electrospun fibers | |
| CN111962210B (en) | Polycaprolactone/methacryloylated elastin nanofiber composite membrane and preparation method and application thereof | |
| Coelho et al. | Electrospinning technology: designing nanofibers toward wound healing application | |
| CN110464866A (en) | A kind of nucleocapsid medicament-carrying nano-fiber dressing and preparation method thereof | |
| KR101677979B1 (en) | Hydrogel composite comprising nanocellulose and fabrication method thereof | |
| CN111617303A (en) | Preparation method of high-strength nanofiber membrane wound dressing | |
| Ghasemkhah et al. | Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches | |
| Mohseni et al. | Highly conductive self-electrical stimuli core-shell conduit based on PVDF-chitosan–gelatin filled with in-situ gellan gum as a possible candidate for nerve regeneration: a rheological, electrical, and structural study | |
| Yan et al. | Dual drug-loaded core-shell nanofibers membranes via emulsion electrospinning and their controllable sustained release property | |
| Zhang et al. | Cellulose nanofibers electrospun from aqueous conditions | |
| Abdelhakeem et al. | State-of-the-Art Review of Advanced Electrospun Nanofiber Composites for Enhanced Wound Healing | |
| CN102772828A (en) | Self-regulation high-drug load nano-fiber drug slow-release membrane and preparation method thereof | |
| CN112353780A (en) | Drug sustained and controlled release platform system with double nano composite structures |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190104 |
|
| RJ01 | Rejection of invention patent application after publication |