CN109122297A - A kind of high-quality suitable production resistance to bacterial wilt new product of tobacco selection - Google Patents
A kind of high-quality suitable production resistance to bacterial wilt new product of tobacco selection Download PDFInfo
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- CN109122297A CN109122297A CN201811138172.XA CN201811138172A CN109122297A CN 109122297 A CN109122297 A CN 109122297A CN 201811138172 A CN201811138172 A CN 201811138172A CN 109122297 A CN109122297 A CN 109122297A
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- resistance
- breeding
- strain
- quality
- bacterial wilt
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses a kind of high-quality suitable production resistance to bacterial wilt new product of tobacco selections, and steps are as follows: step 1, the determination of breeding objective and procedure of breeding adjustment；Step 2, sexual hybridization formulated in combination；Step 3, resistance selects；Step 4, segregating generation selection is identified with the generation is stablized；Step 5, resistance standard variety is set up；Step 6, the selection of agronomy, qualitative character is taken into account while resistant strain selects；Step 7, roasting and production matter identification is taken out in the planting density of different generations, fertilization mode, baking.The present invention has the advantages that optimizing current breed breeding sequence；Suitable for standardizing breeding, it is easy to grasp for beginner, is suitable for scale, standardization, sequencing breeding production operation；Conducive to the precision for improving breeding goal and realize probability；It is easily achieved the target of resistance to bacterial wilt High Quality Tobacco breeding of new variety.
Description
Technical field
The present invention relates to tobacco breeding technical field, in particular to a kind of high-quality suitable anti-blueness of production based on field Disease garden identification
Blight new product of tobacco selection.
Background technique
Tobacco is important one of the industrial crops of China, and tobacco bred is the important material base of tobacco leaf production.Tobacco is green
Blight (Tobacco bacterial wilt) is one as caused by Ralstonia solanacearum (Ralstonia solanacearum)
Kind bacterial disease is one of tobacco agriculture production destructive disease.Cultivation High quality and diseases resistance kind is effective and environment is friendly
The method for preventing and treating bacterial wilt well, the breeding paces for accelerating varietal resistance kind be tobacco leaf production to breeder it is urgent highly necessary
It asks.It cultivates Resistance to bacterial wilt tobacco bred and most importantly carries out Resistance Identification (being selected in the case where there is Disease pressure).Tobacco
The Resistance Identification of kind can be identified with Adult plant in seedling stage, can also be divided into indoor Resistance Identification and field resistance identification method, field
Between sick nursery Resistance Identification method can preferably react the resistance of kind, and the economical characters such as its plant type can be investigated, be more
Generally acknowledged tobacco bacterial wilt identifies measure.But tobacco new lines are cultivated at present in Breeding Process generally without Resistance Identification
(in other words to the segregating generation of breeding without Resistance Identification, selection), is substantially carried out the identification of yield and quality, Resistance Identification is often
Progress even breeder is promoting the Resistance to bacterial wilt participated in regional testing fashion and do not know new lines after the completion of being placed on strain breeding
Situation.
In breed breeding, the determination of breeding objective, the selection of parent, breeding technique measure etc. are to realize variety selection
Importance, the invention patent carry out the breeding program of high-quality Resistance to bacterial wilt kind, selection excellent on the basis of existing
Change.
Tobacco breeding target is in Chinese breeding program that is generally high-quality, fitting production, highly resistance, variety certification program and breeder
Also it is carried out according to this program, in Breeding Process based on quality and Yield test, Resistance Identification is often placed on final step
Or do not wait until to identify resistance situation after the strain of breeding is stablized using resistance as breeding goal, thus even if some breedings
Person, which selects Resistance to bacterial wilt kind also, certain contingency.However from the aspect of genetic development, resistance trait generally compared with
Quality and Inheritance of Yield Traits rule are simple, easily operated, and it is same simple and easy character determination to be placed on the status selected first
When take into account quality and produce matter, it may be possible to the better method of the high-quality highly resistance kind of breeding.
Summary of the invention
The present invention in view of the drawbacks of the prior art, proposes a kind of high-quality suitable production resistance to bacterial wilt new product of tobacco selection,
Effective solution the above-mentioned problems of the prior art.
In order to realize the above goal of the invention, the technical solution adopted by the present invention is as follows:
A kind of high-quality suitable production resistance to bacterial wilt new product of tobacco selection, comprising the following steps:
Step 1, the determination of breeding objective and procedure of breeding adjustment；
Breeding objective is divided into two classes:
(1) breeding goal of Resistance to bacterial wilt new material is the polymerization of Resistance to bacterial wilt gene, utilizes the group of resistant gene
Conjunction and reciprocal effects improve resistance intensity and resistant stability broad spectrum activity, and breeding Resistance to bacterial wilt tobacco new lines, are further
It polymerize with other objective traits and material base is provided；
(2) breeding goal of high-quality resistance to bacterial wilt new lines is to carry out improvement breeding to quality and resistance simultaneously, is realized anti-
Property polymerize with good, the high-quality resistance to bacterial wilt new lines of breeding, be tobacco leaf production service.
High-quality resistance breeding program is adjusted to resistance to be selected the first step for being placed on breeding selection, the separation of breeding material
Carry out breeding work in bacterial wilt sick nursery from generation to generation, the acquisition of resistance data is applied in entire Breeding Process.
Step 2, sexual hybridization formulated in combination；
Sexual hybridization formulated in combination mode is selected according to breeding objective, scale, and resistance breeding needs resistant parent
And the parent of other breeding objectives participates in and Cross type appropriate is used to prepare.
Step 3, resistance selects；
In resistant variety Breeding Process, bacterial wilt Development process is tracked closely, adheres to selecting based on strain selection with single plant
It is selected as auxiliary, quantitative target specifically:
Single plant Resistance Identification single plant Resistance Identification uses 6 grades of standards of perfection, is 0 grade, 1 grade, 3 grades, 5 grades, 7 grades, 9 grades；?
Resistance single plant resistance class should be at 3 grades to 0 grade in selection course；
Strain is identified as main strain Resistance Identification using 4 grades of standards of perfection, is highly resistance (HR), anti-(R), susceptible (S), high sense
(HS)；Resistance strain resistance class should be in anti-or highly resistance in the selection process；
The investigation of tobacco bacterial wilt Disease Resistance, resistance partition of the level and statistical method are as follows:
(1) time and content select 1 time, generally 3 times from phase beginning of falling ill every investigation in 10 days.Investigate the hair of bacterial wilt
Sick rate and sick grade.
(2) investigation of investigation standard bacterial wilt incidence is according to " classification of tobacco pest and disease damage and investigation method " (GB/T
23222-2008) standard, (six grades) investigation are classified as unit of strain: 0 grade: complete stool is disease-free；1 grade: occasionally there are chlorisis spot, or disease in stem
1/2 or less blade of side is wilting；3 grades: there is black streak in stem, but high by 1/2 no more than stem, or 1/2 to 2/3 blade of sick side is wilting；5
Grade: basal part of stem black streak is more than that stem is high by 1/2, but does not reach at the top of stem, or sick side 2/3 is wilting with blade；7 grades: stem is black
Vitta spot reaches at the top of stem or diseased plant blade is all wilting；9 grades: diseased plant is substantially withered.
(3) disease incidence, disease index and resistance index calculate disease incidence=(morbidity strain number/investigation total strain number) × 100 diseases
Feelings index [Σ (the diseased plant strain numbers at different levels × disease grade value)/(investigation total strain number × superlative degree value)] × 100 resistance index RI=ln
In [DI/ (100-DI)]-ln [Do/ (100-Do)] formula: DI- tested variety (is) disease index；The disease-resistant check variety state of an illness of Do-
Index.
(4) overall disease resistance evaluation standard evaluates disease resistance, the criteria for classifying on the basis of its resistance index are as follows:≤0 is height
It is anti-, it is indicated with HR；0.01-1.00 is disease-resistant, are indicated with R；1.01~1.49 is susceptible, are indicated with S；>=1.5 be high sense, is used
HS is indicated
Step 4, segregating generation selection is identified with the generation is stablized
The principle that the selection of segregating generation single plant should adhere to all-the-way tracking, repeatedly select, fall ill final final election.
Tobacco is each to be bred in a manner of sexual selfing from generation to generation, until strain biological character stabilization forms strain.In segregating generation
Each biological character is inconsistent in strain, and resistance selects appropriate to the occasion recruit to look into the sick grade of whole single plants in strain, calculates resistance index, really
Determine resistance class and sort, resistance single plant is then selected in the resistance strain for meeting resistance class, and take into account other agronomy
The selection of shape.When the biological character of strain, which is stablized, forms strain, its clear resistance class of the resistance index of the strain is identified, and
It identifies its yield and quality, promotes high Primary Assay identification.
Step 5, resistance standard variety is set up
If tobacco bacterial wilt resistance level is successively are as follows: yellow (height sense) < Yun yan85 (susceptible) < K326 (disease-resistant) < of long neck
K346 (disease-resistant) < inhales No. four (highly resistance) of circle.
Step 6, the selection of agronomy, qualitative character is taken into account while resistant strain selects.
Step 7, roasting and production matter identification is taken out in the planting density of different generations, fertilization mode, baking
1-4 times of Production of Large Fields density is raw as its planting density of the propulsion of generation and fertilization mode move closer to crop field
It produces, the baking that strain is carried out after stable strain to be formed, which is taken out, bakes, and after tentatively specifying the good strain of resistance, carries out high level-one
Production matter identification.
Further, following Cross type is used when resistance to bacterial wilt fine quality is target breeding in step 2:
Single cross mode: (anti-1 × anti-2), (anti-1 × high-quality 1)；
Backcrossing mode: (anti-× high-quality) × high-quality, (anti-1 × anti-2) × anti-2；
Reestablish diplomatic relations mode: (anti-1 × anti-2) × (anti-1 × anti-2), (anti-1 × anti-2) × high-quality, (anti-1 × high-quality 1) × (anti-2 ×
It is high-quality 2), (anti-1 × anti-2) × (high-quality 1 × high-quality 2)；
Illustrate: × indicate hybridization；Resist 1 to indicate resistance parent 1, resists 2 to indicate resistance parents 2, mark 1 or 2 shows the two not
Together；High-quality expression Quality parents, high-quality 1 indicates Quality parents 1, and high-quality 2 indicate Quality parents 2, and mark 1 or 2 shows the two not
Together.
Compared with prior art the present invention has the advantages that 1 selects tobacco bacterial wilt resistance first in the procedure of breeding, then
Selection economical character and quality are taken into account, current breed breeding sequence is optimized, meets the difficulty or ease of the genetic development of each breeding character
Degree sequence, purpose is strong, strong operability；2 be suitable for standardization breeding, be easy to for beginner grasp, be suitable for scale,
Standardization, sequencing breeding production operation；It more conducively improves the precision of breeding goal and realizes probability, to the workshop-based of improvement breeding
Small-scale operations mode is significant；3 based on field test Resistance to bacterial wilt, can be right close to Production of Large Fields situation
Economical character, quality trait carry out simultaneous selection, it is easier to realize the target of resistance to bacterial wilt High Quality Tobacco breeding of new variety.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention more comprehensible, it is exemplified below embodiment, the present invention is done
It is further described.
A kind of high-quality suitable production resistance to bacterial wilt new product of tobacco selection, comprising the following steps:
Step 1, the determination of breeding objective and procedure of breeding adjustment；
Breeding objective is divided into two classes:
(1) breeding goal of Resistance to bacterial wilt new material is the polymerization of Resistance to bacterial wilt gene, utilizes the group of resistant gene
Conjunction and reciprocal effects improve resistance intensity and resistant stability broad spectrum activity, and breeding Resistance to bacterial wilt tobacco new lines, are further
It polymerize with other objective traits and material base is provided；
(2) breeding goal of high-quality resistance to bacterial wilt new lines is to carry out improvement breeding to quality and resistance simultaneously, is realized anti-
Property polymerize with good, the high-quality resistance to bacterial wilt new lines of breeding, be tobacco leaf production service.
It is required that high-quality resistance breeding program is adjusted to resistance to be selected the first step for being placed on breeding selection, point of breeding material
Generation development breeding in bacterial wilt sick nursery of passing away works, and the acquisition of resistance data is applied in entire Breeding Process.Therefore require have one
Determine the tobacco bacterial wilt resistance sick nursery of area.
Step 2, the preparation of sexual hybridization combination；
Sexual hybridization formulated in combination mode is selected according to breeding objective, scale, and resistance breeding needs resistant parent
And the parent of other breeding objectives participates in and Cross type appropriate is used to prepare, resistance to bacterial wilt fine quality is target choosing
Following Cross type can be used when educating:
Single cross mode: (anti-1 × anti-2), (anti-1 × high-quality 1), etc.；
Backcrossing mode: (anti-× high-quality) × high-quality, (anti-1 × anti-2) × anti-2, etc.；
Reestablish diplomatic relations mode: (anti-1 × anti-2) × (anti-1 × anti-2), (anti-1 × anti-2) × high-quality, (anti-1 × high-quality 1) × (anti-2 ×
It is high-quality 2), (anti-1 × anti-2) × (high-quality 1 × high-quality 2), etc.；
Illustrate: in upper example × indicate hybridization；Anti- 1 indicates resistance parent 1, resists 2 to indicate resistance parent 2, mark 1 or 2 shows
The two is different；High-quality expression Quality parents, high-quality 1 indicates Quality parents 1, and high-quality 2 indicate Quality parents 2, and mark 1 or 2 shows
The two is different.
Step 3, resistance selection principle and standard；
In resistant variety Breeding Process, bacterial wilt Development process should be tracked closely, adhered to based on strain selection, with single plant
Supplemented by selection, quantitative target specifically:
Single plant Resistance Identification single plant Resistance Identification uses 6 grades of standards of perfection, is 0 grade, 1 grade, 3 grades, 5 grades, 7 grades, 9 grades；?
Resistance single plant resistance class should be at 3 grades to 0 grade in selection course；
Strain is identified as main strain Resistance Identification using 4 grades of standards of perfection, is highly resistance (HR), anti-(R), susceptible (S), high sense
(HS)；Resistance strain resistance class should be in anti-or highly resistance in the selection process；
The sick grade of the above single plant or strain is a dynamic data in bacterial wilt Development process, and different onset period, it was exhausted
Logarithm is different, and grade scale should select to reach 9 grades or its group's disease index in the single plant disease grade of the susceptible long neck Huang of control
Investigation period when reaching 100 is advisable.
The single plant, the phenotypic number that strain (they are in segregating generation) Resistant index is the respondent, the number between single plant
Different value difference includes genetic variety and environmental difference；And stablize strain (or kind) because the genotype between single plant is consistent
, therefore resistance numerical value difference only includes environmental difference between the single plant of single plant.Fully understand the meaning of these numerical value differences to guidance
Breeder, which carries out effective field breeding selection, more important meaning.
The investigation of tobacco bacterial wilt Disease Resistance, resistance partition of the level and statistical method are referring to as follows:
(1) time and content select 1 time, generally 3 times from phase beginning of falling ill every investigation in 10 days.Investigate the hair of bacterial wilt
Sick rate and sick grade.
(2) investigation of investigation standard bacterial wilt incidence is according to " classification of tobacco pest and disease damage and investigation method " (GB/T
23222-2008) standard, (six grades) investigation are classified as unit of strain: 0 grade: complete stool is disease-free；1 grade: occasionally there are chlorisis spot, or disease in stem
1/2 or less blade of side is wilting；3 grades: there is black streak in stem, but high by 1/2 no more than stem, or 1/2 to 2/3 blade of sick side is wilting；5
Grade: basal part of stem black streak is more than that stem is high by 1/2, but does not reach at the top of stem, or sick side 2/3 is wilting with blade；7 grades: stem is black
Vitta spot reaches at the top of stem or diseased plant blade is all wilting；9 grades: diseased plant is substantially withered.
(3) disease incidence, disease index and resistance index calculate disease incidence=(morbidity strain number/investigation total strain number) × 100 diseases
Feelings index [Σ (the diseased plant strain numbers at different levels × disease grade value)/(investigation total strain number × superlative degree value)] × 100 resistance index RI=ln
In [DI/ (100-DI)]-ln [Do/ (100-Do)] formula: DI- tested variety (is) disease index；The disease-resistant check variety state of an illness of Do-
Index.
(4) overall disease resistance evaluation standard evaluates disease resistance, the criteria for classifying on the basis of its resistance index are as follows:≤0 is height
It is anti-, it is indicated with HR；0.01-1.00 is disease-resistant, are indicated with R；1.01~1.49 is susceptible, are indicated with S；>=1.5 be high sense, is used
HS is indicated
Step 4, segregating generation selection is identified with the generation is stablized
The principle that the selection of segregating generation single plant should adhere to all-the-way tracking, repeatedly select, fall ill final final election.
Tobacco is each to be bred in a manner of sexual selfing from generation to generation, until strain biological character stabilization forms strain.In segregating generation
Each biological character is inconsistent in strain, and resistance selects appropriate to the occasion recruit to look into the sick grade of whole single plants in strain, calculates resistance index, really
Determine resistance class and sort, resistance single plant is then selected in the resistance strain for meeting resistance class, and take into account other agronomy
The selection of shape.When the biological character of strain, which is stablized, forms strain, its clear resistance class of the resistance index of the strain is identified, and
It identifies its yield and quality, promotes high Primary Assay identification (such as provincial or national regional testing).
Step 5, resistance standard variety is set up
Since sick nursery is affected by a natural in resistant strain selection course, the grade of Resistance Identification is in sick nursery year
Between, even can all fluctuate in plot between plot, standard resistant variety is set as providing in terms of selecting consistency
Reliable foundation favorably improves the validity of selection；The invention patent people specifies that 5 parts of Tobacco Germplasm Resources are it according to test data
Standard, tobacco bacterial wilt resistance level is successively are as follows: yellow (height sense) < Yun yan85 (susceptible) < K326 (disease-resistant) the < K346 of long neck
(disease-resistant) < inhales No. four (highly resistance) of circle.
Step 6, plant type, blade profile, the number of sheets, strain are taken into account while resistant strain selects falls the agronomy such as Huang, qualitative character
Selection
The final requirement in agricultural production of tobacco bred be to provide it is suitable produce good agricultural product, adopt resistant variety into
One of the condition that row plantation only provides safeguard, therefore select to carry out economical character selection while resistance to be successful resistance product
The essential condition of kind breeding.The plant type of the clear cigarette strain of the invention patent, blade profile, the number of sheets, tobacco leaf fall agronomy such as yellow situation, qualitative
Shape is important field selection traits, should be selected simultaneously.
Step 7, roasting and production matter identification is taken out in the planting density of different generations, fertilization mode, baking
The invention patent is placed on resistance choosing the first step (first generation point i.e. after the completion of cross combination preparation of the procedure of breeding
In generation of passing away, begins to execute), planting type in sick nursery is referring to patent of invention " tobacco bacterial wilt disease resistance field high density
Identification method " ZL200910144985.4 requires variation for 1-4 times of Production of Large Fields density with the generation according to test material
Its planting density and fertilization mode is promoted to move closer to Production of Large Fields, the baking that strain is carried out after stable strain to be formed, which is taken out, bakes
(as only toasted to middle part tobacco leaf) after tentatively specifying the good strain of resistance, carries out higher leveled production matter and identifies (such as product
It is comparative test), selection while such arrangement is conducive to make full use of limited sick nursery and take into account resistance and economical character.
Embodiment 1: since 2008, carry out tobacco bacterial wilt in the town the Han Ting village Yi Xing of local dwelling in Wannan Xuancheng City of Anhui Province
Resistant variety identification and breeding of new variety research, " tobacco bacterial wilt is disease-resistant referring to patent of invention for the planting type in sick nursery
Property field high density identification method " ZL200910144985.4, and use south numerous added-generation mode.It is high-quality with breeding Resistance to bacterial wilt
For the purpose of suitable production tobacco bred.First using Hongda tobacco, K326 as Quality parents, K346, inhale circle No. four for resistance parent into
The combination of row preparing hybrid, forms single cross or the F1 that reestablishes diplomatic relations, in its Resistance to bacterial wilt of sick nursery Preliminary Identification, and harvests Seed Development F2
(segregating generation)；The selection for having carried out resistance single plant for 2009 in sick nursery plantation, the planting type in sick nursery are special referring to invention
Benefit " ralstonia solanacearum disease resistance high density identification method in field " ZL200910144985.4, density are 4 times of Production of Large Fields,
Resistance standard variety is uniformly arranged in sick nursery, until the morbidity later period selects to reach 3 grades of resistance or more 1490 plants of single plants altogether and be selfed
It reserves seed for planting to form strain, 1490 parts of strain south numerous added-generations of current year (add for when to select based on economical character) eliminate the poor strain of plant type,
1050 parts of strains are selected altogether；1050 parts of strains in 2010 are planted in sick nursery and are selected, and planting type such as 2009, every strain plantation 32
Strain investigation strain bacterial wilt onset state simultaneously sorts, and resistance single plant is selected in resistance strain and takes into account economical character, obtains 856
Part resistance single plant, the second half year have carried out south numerous added-generation and have accelerated strain homozygous and carry out selecting the 648 parts of strains of total harvest to economical character
System；Continuation sick nursery selection in 2011 and south numerous added-generation, until numerous the planted strain of end in south has reached F7 generation, still separation strains continue to select,
Part combination has generated stable strain, selects within 2012 stable strain to participate in strain and takes out roasting identification；It so executes to 2015 and passes through
Resistance Identification produces matter identification, industrial enterprise sensory evaluating smoking, Chemical bath deposition, has selected A12, A29, A28, cigarette 240, A10
Etc. more parts resistance High Quality Tobacco new lines.
Those of ordinary skill in the art will understand that the embodiments described herein, which is to help reader, understands this hair
Bright implementation method, it should be understood that protection scope of the present invention is not limited to such specific embodiments and embodiments.Ability
The those of ordinary skill in domain disclosed the technical disclosures can make its various for not departing from essence of the invention according to the present invention
Its various specific variations and combinations, these variations and combinations are still within the scope of the present invention.
Claims (2)
1. a kind of high-quality suitable production resistance to bacterial wilt new product of tobacco selection, which comprises the following steps:
Step 1, the determination of breeding objective and procedure of breeding adjustment；
Breeding objective is divided into two classes:
(1) breeding goal of Resistance to bacterial wilt new material be Resistance to bacterial wilt gene polymerization, using resistant gene combination with
Reciprocal effects, improve resistance intensity and resistant stability broad spectrum activity, breeding Resistance to bacterial wilt tobacco new lines, for further with its
The polymerization of its objective trait provides material base；
(2) breeding goal of high-quality resistance to bacterial wilt new lines is to carry out improvement breeding to quality and resistance simultaneously, realize resistance with
Good polymerization, the high-quality resistance to bacterial wilt new lines of breeding are tobacco leaf production service；
High-quality resistance breeding program is adjusted to resistance to be selected the first step for being placed on breeding selection, the segregating generation of breeding material
Carry out breeding work in bacterial wilt sick nursery, the acquisition of resistance data is applied in entire Breeding Process；
Step 2, sexual hybridization formulated in combination；
Sexual hybridization formulated in combination mode is selected according to breeding objective, scale, resistance breeding need resistant parent and its
The parent of its breeding objective participates in and Cross type appropriate is used to prepare；
Step 3, resistance selects；
In resistant variety Breeding Process, closely track bacterial wilt Development process, adhere to strain selection based on, be with Single-plant selection
It is auxiliary, quantitative target specifically:
Single plant Resistance Identification single plant Resistance Identification uses 6 grades of standards of perfection, is 0 grade, 1 grade, 3 grades, 5 grades, 7 grades, 9 grades；It is selecting
Resistance single plant resistance class should be at 3 grades to 0 grade in the process；
Strain is identified as main strain Resistance Identification using 4 grades of standards of perfection, is highly resistance (HR), anti-(R), susceptible (S), high sense
(HS)；Resistance strain resistance class should be in anti-or highly resistance in the selection process；
The investigation of tobacco bacterial wilt Disease Resistance, resistance partition of the level and statistical method are as follows:
(1) time and content select 1 time, generally 3 times from phase beginning of falling ill every investigation in 10 days；Investigate the disease incidence of bacterial wilt
With sick grade；
(2) investigation of investigation standard bacterial wilt incidence is according to " classification of tobacco pest and disease damage and investigation method " (GB/T 23222-
2008) standard, (six grades) investigation are classified as unit of strain: 0 grade: complete stool is disease-free；1 grade: occasionally there are chlorisis spot, or sick side 1/2 in stem
Following blade is wilting；3 grades: there is black streak in stem, but high by 1/2 no more than stem, or 1/2 to 2/3 blade of sick side is wilting；5 grades: stem
Base portion black streak is more than that stem is high by 1/2, but does not reach at the top of stem, or sick side 2/3 is wilting with blade；7 grades: stem's black streak
It reaches at the top of stem or diseased plant blade is all wilting；9 grades: diseased plant is substantially withered；
(3) disease incidence, disease index and resistance index calculate disease incidence=(morbidity strain number/investigation total strain number) × 100 state of an illness and refer to
Number [Σ (the diseased plant strain numbers at different levels × disease grade value)/(investigation total strain number × superlative degree value)] × 100 resistance index RI=ln [DI/
(100-DI)] in-ln [Do/ (100-Do)] formula: DI- tested variety (is) disease index；The disease-resistant check variety state of an illness of Do- refers to
Number；
(4) overall disease resistance evaluation standard evaluates disease resistance, the criteria for classifying on the basis of its resistance index are as follows: and≤0 is highly resistance,
It is indicated with HR；0.01-1.00 is disease-resistant, are indicated with R；1.01~1.49 is susceptible, are indicated with S；>=1.5 be high sense, with HS table
Show
Step 4, segregating generation selection is identified with the generation is stablized
The principle that the selection of segregating generation single plant should adhere to all-the-way tracking, repeatedly select, fall ill final final election；
Tobacco is each to be bred in a manner of sexual selfing from generation to generation, until strain biological character stabilization forms strain；Strain in segregating generation
Interior each biological character is inconsistent, and resistance selects appropriate to the occasion recruit to look into the sick grade of whole single plants in strain, calculates resistance index, determines anti-
Property grade and sort, resistance single plant is then selected in the resistance strain for meeting resistance class, and take into account other economical characters
Selection；When the biological character of strain, which is stablized, forms strain, its clear resistance class of the resistance index of the strain is identified, and identify
Its yield and quality promote high Primary Assay identification；
Step 5, resistance standard variety is set up
If tobacco bacterial wilt resistance level is successively are as follows: yellow (height sense) < Yun yan85 (susceptible) < K326 (disease-resistant) the < K346 of long neck
(disease-resistant) < inhales No. four (highly resistance) of circle；
Step 6, the selection of agronomy, qualitative character is taken into account while resistant strain selects；
Step 7, roasting and production matter identification is taken out in the planting density of different generations, fertilization mode, baking；
1-4 times of Production of Large Fields density moves closer to Production of Large Fields with its planting density of the propulsion of generation and fertilization mode, to
It is formed after stable strain and carries out the baking of strain and take out roasting, after tentatively specifying the good strain of resistance, carry out higher leveled production
Matter identification.
2. according to the method described in claim 1, it is characterized by: when resistance to bacterial wilt fine quality is target breeding in step 2
Using following Cross type:
Single cross mode: (anti-1 × anti-2), (anti-1 × high-quality 1)；
Backcrossing mode: (anti-× high-quality) × high-quality, (anti-1 × anti-2) × anti-2；
Reestablish diplomatic relations mode: (anti-1 × anti-2) × (anti-1 × anti-2), (anti-1 × anti-2) × high-quality, (anti-1 × high-quality 1) × (anti-2 × high-quality
2), (anti-1 × anti-2) × (high-quality 1 × high-quality 2)；
Illustrate: × indicate hybridization；Resist 1 to indicate resistance parent 1, resists 2 to indicate resistance parents 2, mark 1 or 2 shows that the two is different；It is excellent
Matter indicates Quality parents, and high-quality 1 indicates Quality parents 1, and high-quality 2 indicate Quality parents 2, and mark 1 or 2 shows that the two is different.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811138172.XA CN109122297A (en) | 2018-09-28 | 2018-09-28 | A kind of high-quality suitable production resistance to bacterial wilt new product of tobacco selection |
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| CN201811138172.XA CN109122297A (en) | 2018-09-28 | 2018-09-28 | A kind of high-quality suitable production resistance to bacterial wilt new product of tobacco selection |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106376329A (en) * | 2016-08-30 | 2017-02-08 | 中国烟草总公司广东省公司 | Field disease nursery identifying method for disease resisting strength of tobacco resisting bacterial wilt |
| US20180271042A1 (en) * | 2004-08-26 | 2018-09-27 | Monsanto Technology Llc | Methods Of Seed Breeding Using High Throughput Nondestructive Seed Sampling |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180271042A1 (en) * | 2004-08-26 | 2018-09-27 | Monsanto Technology Llc | Methods Of Seed Breeding Using High Throughput Nondestructive Seed Sampling |
| CN106376329A (en) * | 2016-08-30 | 2017-02-08 | 中国烟草总公司广东省公司 | Field disease nursery identifying method for disease resisting strength of tobacco resisting bacterial wilt |
Non-Patent Citations (5)
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| 佟道儒等: "《烟草育种学》", 31 December 1997 * |
| 兰俊荣等: "部分烟草种质资源的青枯病抗性鉴定", 《福建农业学报》 * |
| 孙学永等: "高密度栽培对烟草青枯病抗性鉴定及株型性状的影响", 《中国烟草学报》 * |
| 湖北省烟草科研所编: "《湖北省烟草生产技术培训教程》", 31 October 2013 * |
| 苏德成等: "《中国烟草栽培学》", 30 November 2005 * |
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