CN109111575A - A kind of preparation method and application of metal-organic framework nano particle - Google Patents
A kind of preparation method and application of metal-organic framework nano particle Download PDFInfo
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- CN109111575A CN109111575A CN201810503899.7A CN201810503899A CN109111575A CN 109111575 A CN109111575 A CN 109111575A CN 201810503899 A CN201810503899 A CN 201810503899A CN 109111575 A CN109111575 A CN 109111575A
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- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Abstract
The present invention discloses a kind of preparation method and application of metal-organic framework nano particle, the method is to dissolve molysite with dehydrated alcohol, glutinous health acid is diluted with dehydrated alcohol again after being dissolved with DMF, then above two solution is stirred and evenly mixed, 50~150 °C of 1~60min of microwave heating, it is centrifuged, washs after natural cooling, freeze-drying obtains MOF nano particle.For the present invention compared with cationic compound and cationic-liposome, compound preparation is simple, enormously simplifies preparation flow, improves yield, and avoid using expensive phosphatide, reduces costs.The MOF nano particle good biocompatibility being prepared, it is degradable in vivo, toxic side effect will not be caused in vivo, low concentration MOF even can promote cell Proliferation.By the way that minicircle dna-MOF solution is injected intraperitoneally, it can be hopeful to treat abdomen organ's lesion or tumour, there is biggish application prospect in intraperitoneal expression target gene.
Description
Technical field
The invention belongs to biomedical engineering technology fields.More particularly, to a kind of metal-organic framework nano particle
Preparation method and application.
Background technique
Gene therapy, which refers to, imports target cell, disease caused by therapeutic gene defect or gene unconventionality for foreign gene
Disease.But gene itself, which can not be successfully, to be entered in target cell, it is necessary to rely on genophore.Virus is that a kind of common gene carries
Body, including adenovirus vector (AV), gland relevant viral vector (AAV), retrovirus etc., it is using virus to host cell
Foreign gene is imported intracellular and high efficient expression by infection.Although viral vectors transfection efficiency with higher, viral vectors
It is limited to load gene content, and there is immunogenicity, there are potential biological safety hidden danger.In recent years, minicircle dna
The appearance of (Minicircle DNA, mcDNA), becomes the spotlight in genophore, and minicircle dna is a cricoid expression
Box is the product by DNA recombinant technique, after the skeleton DNA of standard plasmid is removed, contain only a gene expression frame and
There is no external bacterial backbone sequence, its advantage is that can effectively extend the gene of target gene expression time in the cell, carrying
Amount is big and almost without toxicity.But since exposed DNA can be rapidly cleared in humans in physiological environment, it is thin target cannot to be efficiently entering
Born of the same parents still need to suitable delivery system for minicircle dna and are delivered to target tissue or organ.
Common Gene delivery systems include calcium phosphate, cationic-liposome, cationic polymer (polyethyleneimine PEI,
Dendrimer PAMAM) etc..This kind of delivery system immunogenicity is lower, gene struck capacity is big, but transfection efficiency is low, greatly
Its clinical application is limited greatly.It is in the prior art in vivo virus-free rotaring dyeing technology generally by cationic-liposome or sun from
Sub- polymer mediates, and target gene is delivered to intracorporeal organ or tissue.But cationic-liposome expensive starting materials and toxicity compared with
Greatly.Cationic polymer generally comprises polyethyleneimine (PEI), dendrimer, poly- amino ester etc., is inhaled by positive and negative charge
Draw package DNA, commercial reagents such as the in vivo-jetPEI series such as Polyplus company.Cationic-liposome and cation
Transfection efficiency is very high in cell line in vitro for polymer, but transfection efficiency is not high in vivo and bio-toxicity is big,
Therefore, efficiently, the Gene delivery systems with good biocompatibility and with targeting are able to solve base for research and development
Because of the critical issue of clinical drug application.
Metal-organic framework material (Metal Organic Frameworks, MOF) is called the polymerization of metal organic coordination
Object is a kind of novel functionalized crystal material.It is by organic bridging ligands by way of coordinate bond by inorganic metal center
Connect the crystalline material to form the network reticular structure infinitely extended.Due to the structure of organic and inorganic heterogeneity composition
Difference makes its various structures and is adjusted, and has potential application in many aspects.Such as store and separate gas, Yi Jihua
All there are many study for the application of sensing, optics, catalysis aspect.The MOF material of certain structure has biocompatibility again and can
Degradability, therefore also have potential application prospect in biology direction, application of the MOF in terms of drug delivery and bio-imaging is
It has been reported that.
But currently, not yet there is the report for being used to transfect in minicircle dna body by metal organic nanometer granule.
Summary of the invention
The technical problem to be solved by the present invention is to overcome minicircle dna external and in vivo effectively defect present in delivering and
A kind of metal-organic framework nano particle is prepared by microwave solvothermal in deficiency, and the metallo-organic nanoparticle is raw
Object compatibility is good, degradable in vivo, will not cause toxic side effect in vivo, and when low concentration can even promote cell Proliferation.Institute
Stating metal-organic framework nano particle can mediate minicircle dna in intraperitoneal high-efficiency transfection, in intraperitoneal expression target gene egg
It is white, to treat abdomen organ's lesion or tumour.
The object of the present invention is to provide a kind of preparation methods of metal-organic framework nano particle.
The second object of the present invention is to provide the metal-organic framework nano particle and is mediating in genosome in transfection
Using.
Above-mentioned purpose of the invention is to give realization by the following technical programs:
A kind of preparation method of metal-organic framework nano particle, molysite is dissolved with dehydrated alcohol, and glutinous health acid is dissolved with DMF
It is diluted with dehydrated alcohol, then stirs and evenly mixs above two solution again afterwards, 50~150 °C of 1~60min of microwave heating are natural
It is centrifuged, washs after cooling, freeze-drying obtains MOF nano particle.
Preferably, the molysite can be ferric chloride hexahydrate, anhydrous ferric chloride, four Ferric Chloride Hydrateds or ferric nitrate, but
The present invention is not limited to this.
Preferably, the glutinous health acid is that trans-trans stick health acid.
Preferably, the molar ratio of the molysite and muconic acid is 1:0.1~10.
Preferably, the condition of the hydro-thermal reaction is 100 °C of microwave heating 60min.
Preferably, the centrifugation is that 14000g is centrifuged 10min.
Specifically, the preparation method of metal-organic framework nano particle includes the following steps:
S1. ferric chloride hexahydrate is dissolved into sewage ethyl alcohol, obtains yellow transparent solution A；
S2. will be anti-, trans- muconic acid is added in dimethylformamide (DMF), stirs to being completely dissolved, it is molten to obtain clear
Liquid, then plus dehydrated alcohol dilution, obtain solution B；
S3. solution A is transferred in solution B, is sufficiently stirred 3 minutes, obtains solution C；
S4. solution C is poured into microwave hydrothermal reaction kettle, 100 °C, microwave heating 60min, wait solution naturally cool to 60 °C with
It will be taken out when lower；
S5. above-mentioned solution is centrifuged at 14000g 10min, then is washed three times with dehydrated alcohol, finally freeze-drying obtains MOF
Nano particle.
Meanwhile the metal-organic framework nano particle that above-mentioned preparation method is prepared is also claimed in the present invention, be by
Metal ion (Fe) and anti-, trans- muconic acid is coordinated to form the secondary construction unit (SBU) with octahedra rock-steady structure, secondary
Structural unit constructs solid structure again.By the effect link of chemical coordination key between Fe ion and organic matter, and this active force exists
It can be dissociated under aqueous conditions.So iron-based MOF mentioned in the present invention, it all can be with degradation in vivo.With general
From the point of view of the degradation rule of Nano/micron material, degradation rate is related with conditions such as granular size and porositys, and particle is bigger, drop
Solution speed is slower, and porosity is higher, and degradation speed is faster.
The research of the invention finds that the MOF nano particle good biocompatibility being prepared, degradable in vivo, it will not be in body
Inside cause toxic side effect, when low concentration, even can promote cell Proliferation, be it is a kind of can be with the MOF material of mediated gene transfection；
Mixed by the metal-organic framework nano particle that will be prepared with DNA, by intraperitoneal injection enter in vivo, can high-efficiency transfection,
It is intraperitoneal to express DNA product.
Therefore, the present invention also protects metal-organic framework nano particle mediating the application in genosome in transfection.
Meanwhile the metal-organic framework nano particle is also claimed in preparing minicircle dna transfecting formulations in the present invention
Application.
Specifically, the application is that metal-organic framework nano particle is dispersed in PBS, then mixes with minicircle dna.
More specifically, MOF particulate samples are dispersed in water, ultrasound 10~20 minutes forms solution A；Target will be had
The minicircle dna of expressing gene is dispersed in water, and forms B solution；B solution is added in solution A, forms C solution to get micro-loop
DNA transfecting formulations.
Preferably, the mass ratio of the metal-organic framework nano particle and minicircle dna is 0.5~1mg:20 μ g.
The research of the invention finds that it is different from MOF load medicine, when carrying medicine with MOF, needs the specific surface area of MOF bigger, pass through
Physical absorption or the effect of chemical bond and drug molecule combine, to achieve the purpose that carry medicine.But when MOF mediated dna is in abdomen
Intracavitary transfection needs the two-dimensional materials such as rodlike, needle-shaped, can the intraperitoneal mesothelial cell of minimal irritation, reinforce cell to DNA's
Phagocytosis, to promote to transfect.
Compared with prior art, the invention has the following advantages:
Metal-organic framework nano particle of the invention is compared with cationic compound and cationic-liposome, compound preparation
Simply, preparation flow is enormously simplified, yield is improved, and is avoided using expensive phosphatide, is reduced costs, is led to
Temperature and/or the time for crossing control hydro-thermal reaction, metal organic framework compound partial size and pattern can be regulated and controled.It is prepared
MOF nano particle good biocompatibility, it is degradable in vivo, toxic side effect will not be caused in vivo, low concentration MOF can even promote
Into cell Proliferation.By the way that minicircle dna-MOF solution is injected intraperitoneally, can be hopeful to treat abdomen in intraperitoneal expression target gene
Portion's organ lesion or tumour have biggish application prospect.
Detailed description of the invention
Fig. 1 is the scanning electron microscope diagram piece of sample.Wherein A is sample 10, and B is sample 8, and C is sample 1.
Fig. 2 is the particle diameter distribution picture of sample 1.
Fig. 3 is cell survival rate after machine metal framework compound sample 1 and HEPG2 cell co-cultivation 24 as a child.
Fig. 4 is after sample 1 is mixed with mcDNA-Luc by being injected intraperitoneally in Mice Body.IVIS petty action is used after a period of time
Object imager observes mcDNA-Luc in the intracorporal transfected condition of mouse.
Specific embodiment
The present invention is further illustrated below in conjunction with Figure of description and specific embodiment, but embodiment is not to the present invention
It limits in any form.Unless stated otherwise, the present invention uses reagent, method and apparatus routinely try for the art
Agent, method and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
Embodiment 1
A kind of preparation method of metal-organic framework nano particle, includes the following steps:
S1. 0.277g ferric chloride hexahydrate is dissolved into 10ml ethyl alcohol, obtains yellow transparent solution A；
S2. 0.277g trans-trans are sticked health acid to add 3mL dimethylformamide (DMF), stirs to being completely dissolved, is clarified
Clear solution, then plus 10mL ethyl alcohol, obtain solution B；
S3. solution A is transferred in solution B, is sufficiently stirred 3 minutes, obtains solution C；
S4. solution C is poured into anti-microwave hydrothermal to answer in kettle, 100 °C, microwave heating 60min, wait solution naturally cool to 60 °C with
It will be taken out when lower；
S5. above-mentioned solution is centrifuged at 14000g 10min, then is washed three times with dehydrated alcohol, finally freeze-drying obtains MOF
Nano particle；This sample is named as sample 1.
Embodiment 2
A kind of preparation method of metal-organic framework nano particle, it is micro- in addition to the condition of step S4 microwave hydrothermal reaction is 80 °C
Outside Wave heating 1min, other conditions are substantially the same manner as Example 1；MOF nano particle is prepared, this sample is named as sample
2。
Embodiment 3
A kind of preparation method of metal-organic framework nano particle, it is micro- in addition to the condition of step S4 microwave hydrothermal reaction is 90 °C
Outside Wave heating 1min, other conditions are substantially the same manner as Example 1；MOF nano particle is prepared, this sample is named as sample
3。
Embodiment 4
A kind of preparation method of metal-organic framework nano particle, in addition to the condition of step S4 microwave hydrothermal reaction is 100 °C,
Outside microwave heating 5min, other conditions are substantially the same manner as Example 1；MOF nano particle is prepared, this sample is named as sample
Product 4.
Embodiment 5
A kind of preparation method of metal-organic framework nano particle, it is micro- in addition to the condition of step S4 microwave hydrothermal reaction is 70 °C
Outside Wave heating 1min, other conditions are substantially the same manner as Example 1；MOF nano particle is prepared, this sample is named as sample
5。
Embodiment 6
A kind of preparation method of metal-organic framework nano particle, in addition to the condition of step S4 microwave hydrothermal reaction is 100 °C,
Outside microwave heating 30min, other conditions are substantially the same manner as Example 1；MOF nano particle is prepared, this sample is named as
Sample 6.
Embodiment 7
A kind of preparation method of metal-organic framework nano particle, in addition to the condition of step S4 microwave hydrothermal reaction is 100 °C,
Outside microwave heating 10min, other conditions are substantially the same manner as Example 1；MOF nano particle is prepared, this sample is named as
Sample 7.
Embodiment 8
A kind of preparation method of metal-organic framework nano particle, in addition to the condition of step S4 microwave hydrothermal reaction is 150 °C,
Outside microwave heating 1min, other conditions are substantially the same manner as Example 1；MOF nano particle is prepared, this sample is named as sample
Product 8.
Embodiment 9
A kind of preparation method of metal-organic framework nano particle, in addition to the condition of step S4 microwave hydrothermal reaction is 100 °C,
Outside microwave heating 10min, other conditions are substantially the same manner as Example 1；MOF nano particle is prepared, this sample is named as
Sample 9.
Embodiment 10
A kind of preparation method of metal-organic framework nano particle, it is micro- in addition to the condition of step S4 microwave hydrothermal reaction is 50 °C
Outside Wave heating 1min, other conditions are substantially the same manner as Example 1；MOF nano particle is prepared, this sample is named as sample
10。
Embodiment 11
A kind of preparation method of metal-organic framework nano particle, in addition to the condition of step S4 microwave hydrothermal reaction is 100 °C,
Outside microwave heating 1min, other conditions are substantially the same manner as Example 1；MOF nano particle is prepared, this sample is named as sample
Product 11.
Performance test
One, MOF sample characterization is tested
1, transmission electron microscope (TEM) is observed: the above-mentioned sample 1~11 being prepared being dispersed in alcoholic solution, ultrasound
10min or more, until sample is completely dispersed.By calcium phosphate sample drop on copper mesh, with FEI Tecnai G2 F20 S-Twin type
Number electron microscope obtains sample transmission photo under 110V voltage, and organic metal framework nano particle size is by Malvern laser
Granularity scatterometer measurement obtains.
2, result is as shown in Figure 1, can prepare different-shape and ruler by reaction time or the difference of reaction temperature
Very little sample, the reaction time is longer, and the partial size for obtaining MOF sample is bigger, and maximum can big 1 microns.But reaction temperature pair
Sample particle diameter influences less, but has an impact to sample topography, and influences without regularity.100oWhen C reacts 1h, it can be obtained two
The taper compound of head point.The particle diameter distribution result of sample 1 is as shown in Fig. 2, (DLS) the average grain measured by dynamic light scattering
Diameter is 187.8nm, and particle diameter distribution is than more uniform.
Two, MOF sample biocompatibility, degradability experiment
1, sample 1 is configured to various concentration MOF particle to be dispersed in PBS, is co-cultured 24 hours with 5000 HEPG2 cells,
It is handled in this interim liquid that do not change.The cell survival rate of addition various concentration MOF cell is tested after 24 hours by CCK8 kit.
2, result is as shown in Figure 3A, and low concentration to high concentration MOF particle and 293T cell are incubated for 48 hours altogether, does not show
Overt toxicity out.Cell survival rate is 100% or so.More ironically, low concentration MOF can even promote cell Proliferation.From
3B figure is as can be seen that in PBS solution, MOF(sample 1) can degrade generally in 160 hours, and the Fe particle of degradation generation
Will not toxicity be caused to body with organic acid, show that the MOF particle being prepared has good biocompatibility, degradation
Property.Meanwhile the biocompatibility and degradation property of sample 2- sample 11 are good.
Three, MOF sample mediates minicircle dna abdominal cavity transfection (luciferase) experiment
1,1mg MOF particulate samples 1 are dispersed in 200 μ l water, ultrasound 10 minutes forms solution A.20 μ g are had into fluorescein
The minicircle dna of enzyme (luciferase) expressing gene is dispersed in 200 μ l water, forms B solution.B solution is added in solution A,
Form C solution.By C solution by being injected into Mice Body, standby IVIS small animal imaging instrument observation in 24 hours is glimmering
The expression of light element enzyme in vivo.
2, result can be formed apparent as shown in figure 4, by intraperitoneal injection MOF/DNA mixed solution in mouse peritoneal
Gene transfection, transfection efficiency extend at any time and reduce, and transfection time continues at 96 hours or more.It is in experiment it was found that real
Apply the MOF sample in example 1~12 can mediated dna transfect in vivo, this is mainly due to special pointed at both ends of MOF sample
What pattern determined.
Claims (10)
1. a kind of preparation method of metal-organic framework nano particle, which is characterized in that dissolve molysite with dehydrated alcohol, stick
Health acid is diluted with dehydrated alcohol again after being dissolved with DMF, then stirs and evenly mixs above two solution, 50~150 °C of microwave heatings 1
~60min is centrifuged after natural cooling, is washed, and freeze-drying obtains MOF nano particle.
2. preparation method according to claim 1, which is characterized in that the molysite is ferric chloride hexahydrate, anhydrous chlorination
Iron, four Ferric Chloride Hydrateds or ferric nitrate.
3. preparation method according to claim 1, which is characterized in that the glutinous health acid is that trans-trans stick health acid.
4. preparation method according to claim 1, which is characterized in that the molar ratio of the molysite and muconic acid is 1:0.1
~10.
5. preparation method according to claim 1, which is characterized in that the centrifugation is that 14000g is centrifuged 10min.
6. the metal-organic framework nano particle that any one of claim 1 to 5 the method is prepared.
7. metal-organic framework nano particle as claimed in claim 6 is mediating the application in genosome in transfection.
8. metal-organic framework nano particle as claimed in claim 6 is preparing the application in minicircle dna transfecting formulations.
9. application according to claim 8, which is characterized in that by metal-organic framework nano particle described in claim 6
It is dispersed in PBS, then is mixed with minicircle dna.
10. application according to claim 9, which is characterized in that the metal-organic framework nano particle and minicircle dna
Mass ratio be 0.5~1mg:20 μ g.
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| CN110118759A (en) * | 2019-05-06 | 2019-08-13 | 大连理工大学 | A kind of terramycin fluorescence detection method based on surface passivation and DNA covalent coupling modified metal organic backbone nanometer sheet |
| CN110384685A (en) * | 2019-09-04 | 2019-10-29 | 临沂大学 | A kind of metal organic frame pharmaceutical carrier and preparation method thereof of nucleic acid modification |
| CN111948391A (en) * | 2019-05-16 | 2020-11-17 | 南京大学 | Array sensor based on nano metal organic framework for histological diagnosis of colon cancer |
| WO2022164277A1 (en) | 2021-01-28 | 2022-08-04 | 서울대학교병원 | Silicone patch comprising metal-organic framework and silicone composition |
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| CN110118759B (en) * | 2019-05-06 | 2021-09-24 | 大连理工大学 | Oxytetracycline fluorescence detection method based on surface passivation and covalent coupling |
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| KR20220109152A (en) | 2021-01-28 | 2022-08-04 | 서울대학교병원 | Silicone patch comprising metal-organic framework and silicone composition |
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