CN109106985A - A kind of compound rest and its preparation method and application - Google Patents

A kind of compound rest and its preparation method and application Download PDF

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Publication number
CN109106985A
CN109106985A CN201710481947.2A CN201710481947A CN109106985A CN 109106985 A CN109106985 A CN 109106985A CN 201710481947 A CN201710481947 A CN 201710481947A CN 109106985 A CN109106985 A CN 109106985A
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compound rest
penis
pga
pla
cytoskeleton
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Inventor
周广东
肖开颜
夏会堂
袁兆远
陈洁
赵丹丹
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Ninth Peoples Hospital Shanghai Jiaotong University School of Medicine
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Ninth Peoples Hospital Shanghai Jiaotong University School of Medicine
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
    • A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
    • A61L27/46—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with phosphorus-containing inorganic fillers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A61L27/34—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Inorganic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Composite Materials (AREA)
  • Materials Engineering (AREA)
  • Prostheses (AREA)

Abstract

The invention discloses a kind of compound rests and its preparation method and application.The compound rest includes the interior nuclear scaffold for tool people's penis shape that polycaprolactone (PCL)/hydroxyapatite (HA) composite material is formed and polyglycolic acid (PGA)/polylactic acid (PLA) cytoskeleton for being nested on the interior nuclear scaffold.

Description

A kind of compound rest and its preparation method and application
Technical field
The present invention relates to biomaterial and field of tissue engineering technology more particularly to a kind of cartilage supports with people's penis shape Body and preparation method thereof.
Background technique
Penis is the important uropoiesis of male, reproductive organs, has the function of urination, sperm drainage and sexual intercourse.Because of congenital abnormality or The reasons such as wound, tumour lead to penis partially or completely defect, make patient lose urination, potency while, to patient with Come heavy spirit and psychological burden.Defect of penis treatment has important clinic and social value.
Cartilage is the tissue successfully constructed using Method of Tissue Engineering earliest.All kinds of cartilage defects (such as joint, tracheae) Organizational project recovery technique obtain initial success in animal body, or even it is real in Articular cartilage repair field Preliminary clinical conversion is showed.
Polyglycolic acid/polylactic acid (PGA/PLA) is the building common cell scaffold material of tissue engineering bone/cartilage.However, The cartilage mechanical strength deficiency constructed using PGA/PLA as cytoskeleton merely, it is difficult to meet cartilage of penis supporter to intensity Demand；The cartilage of building large volume there is also " hollow " problem, central part subalimentation, it is difficult to form mature cartilage even It necroses.
For " hollow " phenomenon, mechanical strength is insufficient, exact form controls the main of three regeneration cartilage of penis supporters Bottleneck problem, there is an urgent need in the art to provide a kind of three-dimensional compound rest of accurate people's penis shape, and thus obtained people Penis shape cartilage supporter can satisfy the requirement to intensity and center nutrition.
Summary of the invention
The present invention is intended to provide a kind of compound rest that can be used for preparing organizational project people's graft of penis object.
It is a further object to provide the preparation methods of the compound rest.
It is also another object of the present invention to provide the purposes of the compound rest.
In the first aspect of the present invention, a kind of compound rest is provided, the compound rest includes polycaprolactone (PCL)/hydroxyapatite (HA) composite material formed tool people's penis shape interior nuclear scaffold be nested on the interior nuclear scaffold Polyglycolic acid (PGA)/polylactic acid (PLA) cytoskeleton.
In another embodiment, with the total weight of PCL/HA composite material, wherein the HA contained is no more than 30%.
In another embodiment, the interior nuclear scaffold of tool people's penis shape forms for 3D printing.
In another embodiment, the interior nuclear scaffold form of tool people's penis shape is true to nature, and coronary sulcus is obvious；Each position Between meet normal penile ratio, material internal is the minim channel structure that rule is intersected.
In the second aspect of the present invention, a kind of preparation side of compound rest provided present invention as described above is provided Method, the method comprising steps of
(1) the interior nuclear scaffold of one tool people's penis shape is obtained by 3D printing with PCL/HA composite material；
(2) make the pressurization moulding of PGA/PLA timbering material, obtain and interior nuclear scaffold form, the matched cytoskeleton of size；With
(3) cytoskeleton is nested in outside interior nuclear scaffold, the compound rest provided present invention as described above.
In another embodiment, with the former and sun that stamp obtains respectively at left and right sides of internal nuclear scaffold in step (2) Die combination is to the pressurization moulding of PGA/PLA timbering material；The former material is silicon rubber；The formpiston material is gypsum.
In another embodiment, the left and right sides is using median sagittal plane as boundary.
In another embodiment, step (3) is left and right two parts PGA/PLA cytoskeleton by moulding to together in kernel At left and right sides of bracket.
In the third aspect of the present invention, a kind of purposes of compound rest provided present invention as described above, institute are provided It states compound rest and is used for or is used as building organizational project graft of penis object.
In the fourth aspect of the present invention, a kind of organizational project graft of penis object is provided, the graft passes through institute as above The compound rest provided by the invention stated and the cartilage cell for being inoculated in the compound rest are formed.
Accordingly, the present invention provides a kind of three-dimensional compound rests of accurate people's penis shape, and thus obtained people is negative Stem form cartilage supporter can satisfy the requirement to intensity and center nutrition.
Detailed description of the invention
Fig. 1 shows cellular morphology observation and Biocompatibility assessment result；Wherein
A: the PGA fiber of shredding；B: disc PGA/PLA bracket；The electron microscope of C:PGA/PLA bracket；D:P1 is for sucking pig Articular chondrocytes；E: it is inoculated at once, cell material compound；F: the electron microscope after inoculation 3 days.
Fig. 2 shows nuclear scaffold in CAD and 3D printing production penis；Wherein
A: penis data model is rebuild according to penis 3 d scan data；B: hydroxyapatite (HA)；C: polycaprolactone (PCL)；D:PCL and HA mixes (7:3) under 120 DEG C of high temperature；E:3D prints penis model；F: the penis model of completion is printed.
Fig. 3 shows the preparation process of compound rest；Wherein
A:3D prints kernel；B: surface is coated with masking foil；C: the left and right sides is respectively formed former stamp；D: with obtained yin Mould is cast in out gypsum formpiston；E: polyglycolic acid (PGA)；F: compound rest outer layer PGA cytoskeleton；G, H, I: composite support is completed The assembling of frame.
Fig. 4 shows reconstruction process in cartilage body；Wherein
A: compound rest；B: compound rest inoculating cell is at once；C, D: cell-compound rest compound in vitro culture 4 weeks Afterwards；E, F: transplanting in penis shape construction body.
Fig. 5 shows internal 8 weeks tissues substantially sight and histology；Wherein
A: materials are substantially seen；B: along sagittal plane split after substantially sight；C: sample HE dyeing (X40)；D, E, F: sample HE It dyes (X100).
Specific embodiment
After extensive and in-depth study, discovery can use the high-strength at cartilage advantage and PCL of PGA/PLA to inventor The characteristics of degree, easy moulding, production are assembled into the internal penis shape PCL/HA kernel for 3D printing, and outside is coated with preparatory moulding Porous (PGA is fiber, and fibrage mode itself will form porous structure) the PGA/PLA cytoskeleton of thin layer, thus further Construct people's cartilage of penis supporter.The present invention is completed on this basis.
Compound rest
Compound rest provided by the invention has nuclear scaffold and a cytoskeleton in one, and it is interior that the cytoskeleton is coated on this Outside nuclear scaffold；The compound rest has people's penis shape, protrudes at coronary sulcus.
In a kind of better embodiment of the invention, interior nuclear scaffold and cytoskeleton all have people's penis shape.
The building material of the interior nuclear scaffold is polycaprolactone (PCL), is also possible to polycaprolactone (PCL)/hydroxy-apatite Stone (HA) composite material；With polycaprolactone (PCL)/hydroxyapatite (HA) composite material total weight, wherein not containing HA More than 30%；Too fast lower than 20% scaffold degradation it is preferred that 20-30%, being higher than 30% can not 3D because of HA too high levels Printing forms exact form.
The building material of the cytoskeleton is polyglycolic acid (PGA)/polylactic acid (PLA) composite material.
The preparation method of compound rest
Compound rest provided by the invention is prepared by the following method:
The first step, the 3D printing of interior nuclear scaffold；
Second step, the moulding of cytoskeleton；
Third step, the assembling of compound rest.
In one embodiment of the invention, the above-mentioned first step can be according to the three-dimensional under adult male erect penile condition Data, using PCL/HA composite material by 3D printing at nuclear scaffold in people's penis shape of multi-pore channel.The yin that 3D printing is formed Stem form kernel, center be it is solid, outer ring 0.5mm be porous structure, this is the digital mould by being established before 3D printing What type was realized, what early period established is " center is solid, and outer ring is porous " mathematical model, and what is printed is exactly this structure.? In one embodiment of the present invention, above-mentioned second step be within nuclear scaffold the left and right sides difference stamp, obtained former and Formpiston pairing is to obtain cytoskeleton to PGA/PLA composite material pressurization plasticity shape.
In the preferred embodiment of the present invention, the material of former is what elasticity is good, such as, but not limited to, silicon rubber Glue；The material of formpiston be it is scleroid, such as, but not limited to, gypsum.
In the preferred embodiment of the present invention, the cytoskeleton thickness obtained in above-mentioned second step is 1.5 ± 0.5 Millimeter.
It is that the left and right two parts cytoskeleton that will be obtained is nested in the interior nuclear scaffold of first step 3D printing in above-mentioned third step The left and right sides obtains compound rest.
In a better embodiment of the invention, left and right two parts cytoskeleton is nested in the interior of first step 3D printing PLA solution is added dropwise at gap after at left and right sides of nuclear scaffold and imposes appropriate pressure, is fitted gap.
The purposes of compound rest
The penis kernel that 3D printing is formed, center be it is solid, the concentric circles of peripheral thickness 0.5mm is porous structure, should Structure is mainly solid section, ensure that mechanical strength, and outer ring porous structure is conducive to the embedding of kernel and PGA/PLA cytoskeleton It closes, makes the globality of compound rest itself, meanwhile, it is fixed that outer ring porous structure ensure that cell inoculation has cartilage cell in the process Residence and regenerating bone or cartilage make the regenerating bone or cartilage of kernel outer ring and cytoskeleton form continuity, to ensure that final constructed yin The globality of property cartilage supporter.
Compound rest provided by the invention can be used for preparing graft of penis object, in one embodiment of the invention, will Seed cell is inoculated on compound rest provided by the invention, is formed cell-material composite, is cultivated and obtain under suitable condition Organizational project graft of penis object；In the preferred embodiment of the present invention, about 1.5 ± 0.5 millis are regenerated on compound rest Rice cartilage.
The feature that the features described above or embodiment that the present invention mentions are mentioned can be in any combination.Disclosed in this case specification All features can be used in combination with any composition form, each feature disclosed in specification, any can provide it is identical, The alternative characteristics of impartial or similar purpose replace.Therefore except there is special instruction, revealed feature is only impartial or similar spy The general example of sign.
Main advantages of the present invention are:
1, the present invention successfully avoids " hollow " problem.
2,3D printing of the present invention goes out accurate people's penis shape PCL/HA kernel, inside branch of the kernel as tissue engineering bone/cartilage Support, it is sufficient to meet the mechanical strength demand of penis support body.
3, the present invention solves by the preparation of accurate people's penis shape compound rest and constructs yin using tissue engineering technique The form control problem of stem cartilage supporter.
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention Rather than it limits the scope of the invention.In the following examples, the experimental methods for specific conditions are not specified, usually according to conventional strip Part or according to the normal condition proposed by manufacturer.Unless otherwise stated, otherwise all percentages and parts are counted by weight.
Unless otherwise defined, it anticipates known to all professional and scientific terms as used herein and one skilled in the art Justice is identical.In addition, any method similar to or equal to what is recorded and material can be applied to the method for the present invention.Wen Zhong The preferred implement methods and materials are for illustrative purposes only.
Material involved in following embodiments are as follows:
Experimental animal
5 Female nude mices derive from Shanghai Jia Gan Biotechnology Co., Ltd.According to experimental animal in experimentation The requirement of guide is protected to carry out.Every nude mice is implanted into construction in dorsal sc.
Experimental material and equipment
DMEM in high glucose culture medium (Gibco company, the U.S.)；Fetal calf serum, phosphate buffer (Hyclone company, beauty State)；Trypsase (Hyclone company, the U.S.)；II Collagenase Type (Worthington company, the U.S.)；Nonwoven PGA fiber (organizational project National Engineering Research Centre)；Polylactic acid (PLA) (Jinan Dai Gang bioengineering Co., Ltd)；Polycaprolactone (PCL)/hydroxyapatite (HA) composite material (Shanghai Communications University's Materials Academy)；Silicon rubber (Minnesota Mining and Manufacturing Company)；Gypsum is (lucky Log cabin)；The double spray head molding machines (Shanghai Fu Qifan) of 3 D-printing melt-out spinning；Batch mixer (Plastograph EC, Brabender,Germany)。
The acquisition and amplification of primary cell
Cartilage is derived from newborn sucking pig hip, knee joint, and cartilage block is shredded, and 0.25% II Collagenase Type, 37 DEG C of concussions are added 7-8h is digested, is filtered with 100 μm of cell strainers, supernatant is removed in centrifugation.The cell of precipitating is resuspended with DMEM in high glucose culture medium, institute Cell is obtained with 1 × 104cells/cm2Concentration inoculation 100mm culture dish on.In 37 DEG C, 5%CO2And under the conditions of saturated humidity, With containing 10% fetal calf serum, 2ng/mL basic fibroblast growth factor, 1% penicillin and streptomysin DMEM culture medium into Row cultured chondrocytes, amplification, are passed on when chondrocyte growth to 70%-80% merges.
Embodiment 1
Obtain interior nuclear scaffold
(1) CT scan obtains the three-dimensional data under adult male erect penile condition, and carries out data processing.
(2) PCL/HA (7:3) mixing is uniform, is printed as PCL/HA using 3D printer according to the data that processing obtains Nuclear scaffold in people's penis shape.
Interior nuclear scaffold form is true to nature, and coronary sulcus is obvious.Overall length 3cm, meets normal penile ratio between each position, in material Portion is the minim channel structure (Fig. 1) that rule is intersected.
Embodiment 2
The moulding of cytoskeleton and the assembling of compound rest
Nuclear scaffold in the penis shape that printing is completed is covered with masking foil, with the left and right sides point of the internal nuclear scaffold of silicon rubber Other stamp (using median sagittal plane as boundary), is cast in gypsum formpiston with obtained silicon rubber former.
PGA/PLA timbering material Liu Y, Zhang L, Zhou G.In vitro engineering of human ear-shaped cartilage assisted with CAD/CAM technology[J].Biomaterials,2010,31 (8): the technology preparation that 2176-83. is recorded.
Former and formpiston pairing are close, and formpiston gypsum quality is hard and former resin elasticity is good, the two combination, be conducive to pair PGA material pressurization plasticity.The PGA cytoskeleton form that moulding is completed is true to nature, especially prominent at coronary sulcus, and quality such as eggshell is thick It spends 1.5mm (Fig. 3 F).
Left and right two parts PGA/PLA cytoskeleton of moulding is nested in nuclear scaffold or so two in the penis of 3D printing Side is added dropwise PLA solution and imposes appropriate pressure, be fitted gap at the gap of hecatomeral cells bracket pairing.
Compound rest is made of external PGA/PLA cytoskeleton and internal PCL/HA core.Inside and outside two parts matching degree Height is fitted into close.The compound rest form of completion is (Fig. 3 H, I) true to nature.Through over-fitting, left and right two parts cytoskeleton is combined into One, only leaves a light trace (Fig. 3 H).
Embodiment 3
The external structure of cartilage compound
Sucking pig articular chondrocytes P1, concentration 10.0 × 107Cells/ml is inoculated in PGA/PLA-PLA/HA composite support Frame, after 5h, cell sufficiently sticks with material, is added culture solution in vitro culture 4 weeks, changes a not good liquor within every 2 days.
Embodiment 4
Construction implants
Through Medical College, Shanghai Communication Univ.'s animal feeding and using the license of administration committee, nude mice is implemented to anaesthetize, it will Construction is implanted into its dorsal sc, observes the postoperative performance of nude mice.
Construction color, quality are observed, mature cartilage is substantially judged whether it is, whether surface layer cartilage is continuous, and shape maintains Situation opens construction along median sagittal plane, observation cartilage thickness, whether uniform, external cartilage and internal porous PLC/HA's Integration of interface situation.It draws materials within In vivo culture 8 weeks, with 4% paraformaldehyde fixed preparation, the dehydration of 30% sucrose, OCT embedding, frost It is sliced (10 μ m-thick), institutional framework and Extracellular Matrix Secretion situation are observed in row HE dyeing.
As a result
Cellular morphology observation and the assessment of PGA/PLA biocompatibility
Under the microscope, cartilage cell's form is in spindle shape, polygonal to light, is proliferated fast (Fig. 1 D).Cytoskeleton be easy to by Fashion into cylinder, diameter 9mm, with a thickness of 1.5mm.Electron microscopic picture show bracket be in porous structure, PLA enhance PGA it Between crosslinking (Fig. 1 C), PGA/PLA shows good biocompatibility, and cell adherence is on PGA and secretes matrix covering Fiber.
In building process, form maintains compound, and after in vitro culture 4 weeks, surface forms one layer of jejune cartilage Sample tissue.Nude mice by subcutaneous Hui Zhi, postoperative without adverse events such as infection, nude mice performance is active, and wound healing is good, and skin is without breakage. After In vivo culture 8 weeks, nude mice is put to death and drawn materials.(Fig. 4)
In vivo culture is drawn materials after 8 weeks, and sample hard, white chondroid tissue of porcelain for matter, construction surface is smooth, continuous, does not have It is found vascularization.The shapes and sizes of construction are all maintained, compared with compound rest, without obvious on length diameter Variation.Longitudal section is observed, discovery PCL/HA core is wrapped up by cartilage completely, and it is interlaced between two-phase, it combines closely.Cartilage branch Support body row HE after frozen section is dyed.Coloration result shows that penile prosthesis periphery cartilage layers are merged with interior nuclear scaffold, there is part Cartilage cell and fibrous connective tissue grow into internal stent, and peripheral cartilage cell's structure is mature and has lacuna to be formed, and prompt us It is complete that constructed cartilage of penis complex is formed by surrounding cartilage tissue, with the extension of time, can and internal stent Combining closely becomes complete whole.(Fig. 5)
It discusses
PLA embedding PGA has been applied to the building of various forms tissue engineering bone/cartilage as cytoskeleton[9-11].However it is big The building of volume cartilage is faced with " hollow " problem, this is because construction central part, far from surface, nutrition is difficult to penetrate into, interior The acidic metabolite in portion is also difficult to be discharged and accumulate, and leads to central part cell activity variation or even dead, cartilaginous tissue It is downright bad therewith, cavity is ultimately formed, regenerating bone or cartilage is caused to fail.It can be seen that due to the thickness limitation of cartilage building, merely with PGA/ PLA is that material is difficult to construct large volume cartilage.The present invention is only coated with the PGA/PLA with a thickness of 1.5mm in PCL/HA kernel outer layer Cytoskeleton regenerates the cartilage of about 1.5mm thickness, the thickness limitation of regeneration of cartilage is avoided exceeding, to avoid " hollow " problem. Internal 8 weeks histological examination showeds, PCL/HA kernel outer layer regenerate continuous, homogeneous, maturation cartilaginous tissue, successfully avoid " hollow " problem.
The needs whether reconstructed penis has enough intensity to meet sexual life are an important fingers for measuring reconstructed penis Mark, the cartilage mechanical strength deficiency constructed using PGA/PLA as cytoskeleton merely, it is difficult to meet cartilage of penis supporter to intensity Demand.The present invention utilizes the good plasticity of PCL and stronger mechanical property, and 3D printing goes out accurate people's penis shape PCL/HA Kernel, kernel are supported as the inside of tissue engineering bone/cartilage, it is sufficient to meet the mechanical strength demand of penis support body.Penis support The surface layer cartilage of body is fully wrapped around by PCL/HA kernel, is formed and is effectively isolated between interior nuclear scaffold and body, avoids PCL/HA Immune response caused by kernel, while the degradation rate of PCL is effectively slowed down, the long-term retention of PCL/HA ensure that penis supports The long-term maintenance of body intensity.
Currently, be mostly column using the cartilage of penis supporter that tissue engineering technique constructs, no glans penis, coronary sulcus, yin The penises normal anatomical structures such as caulome.This research by normal person's penis carry out laser profile/magnetic resonance imaging,MRI, In conjunction with 3-dimensional reconstruction technology, accurate people's penis shape mathematical model is established, and is prepared accurately based on 3D printing technique People's penis shape PCL/HA kernel.Using digital mould rotating technology, it is coated with PGA/PLA cytoskeleton in PCL/HA kernel outer layer, The compound rest being completed can still keep accurate people's penis shape.By the preparation of accurate people's penis shape compound rest, Solves the form control problem using tissue engineering technique building cartilage of penis supporter.The display of this result of study, three-dimensional are multiple The cartilage supporter for closing 8 weeks in bracket, external structure 4 weeks and nude mouse, all has the penises such as glans penis, coronary sulcus, phallosome Normal anatomical structures realize cartilage of penis support volume morphing control.The core surface of 3D printing has well-regulated cellular structure, Be conducive to outer layer cartilaginous tissue formed with interior nuclear scaffold it is good chimeric, so that penis support body be made to become complete whole.Knot Fruit shows that the tissue engineering bone/cartilage of thin layer is chimeric with kernel close, forms fusion, becomes unified entirety, it is soft to reduce outer layer A possibility that being displaced between bone tissue and kernel.
To sum up, the three-dimensional composite support of accurate people's penis shape can be successfully prepared based on 3D printing and digital mould rotating technology Frame is PCL/HA bracket in conjunction with the renewable kernel out of tissue engineering technique, and outer layer is coated with people's penis shape cartilage of mature cartilage Supporter.
All references mentioned in the present invention is incorporated herein by reference, independent just as each document It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims It encloses.

Claims (10)

1. a kind of compound rest, which is characterized in that the compound rest includes polycaprolactone (PCL)/hydroxyapatite (HA) Composite material formed tool people's penis shape interior nuclear scaffold and be nested on the interior nuclear scaffold polyglycolic acid (PGA)/gather Lactic acid (PLA) cytoskeleton.
2. compound rest as described in claim 1, which is characterized in that with the total weight of PCL/HA composite material, wherein containing Some HA are no more than 30%.
3. compound rest as described in claim 1, which is characterized in that the interior nuclear scaffold of tool people's penis shape is 3D printing It forms.
4. a kind of preparation method of compound rest as described in any one of claims 1-3, which is characterized in that the method Comprising steps of
(1) the interior nuclear scaffold of one tool people's penis shape is obtained by 3D printing with PCL/HA composite material；
(2) make the pressurization moulding of PGA/PLA timbering material, obtain and interior nuclear scaffold form, the matched cytoskeleton of size；With
(3) cytoskeleton is nested in outside interior nuclear scaffold, obtains compound rest as described in any one of claims 1-3.
5. preparation method as claimed in claim 4, which is characterized in that with difference at left and right sides of internal nuclear scaffold in step (2) The former and punch combination that stamp obtains are to the pressurization moulding of PGA/PLA timbering material.
6. preparation method as claimed in claim 5, which is characterized in that the former material is silicon rubber；The formpiston material For gypsum.
7. preparation method as claimed in claim 5, which is characterized in that the left and right sides is using median sagittal plane as boundary.
8. such as the described in any item preparation methods of claim 4-7, which is characterized in that step (3) is by the left and right two of moulding Divide PGA/PLA cytoskeleton to together in the kernel bracket left and right sides.
9. the purposes of compound rest as described in any one of claims 1-3, which is characterized in that be used for or be used as building tissue Engineering graft of penis object.
10. a kind of organizational project graft of penis object, which is characterized in that the graft passes through such as any one of claim 1-3 institute The compound rest stated and the cartilage cell for being inoculated in the compound rest are formed.
CN201710481947.2A 2017-06-22 2017-06-22 A kind of compound rest and its preparation method and application Pending CN109106985A (en)

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