CN109105636A - A method of addition formula microalgae is in chicken feed to prepare high nutrition egg - Google Patents
A method of addition formula microalgae is in chicken feed to prepare high nutrition egg Download PDFInfo
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- CN109105636A CN109105636A CN201811049778.6A CN201811049778A CN109105636A CN 109105636 A CN109105636 A CN 109105636A CN 201811049778 A CN201811049778 A CN 201811049778A CN 109105636 A CN109105636 A CN 109105636A
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- egg
- yolk
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- ratio
- algae
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
Abstract
The present patent application belongs to algae culture technical field, specifically discloses a kind of method of the addition formula microalgae to prepare high nutrition egg in chicken feed, comprising the following steps: (1) dry algae powder is made in algae strain；(2) laying hen will be fed containing the mixed fodder of basal feed and algae powder and harvest egg twice daily, record the daily feed intake of laying hen, laying rate, feedstuff-egg ratio and egg average egg weight；(3) albumen of egg and yolk are separated, compares yolk color, measures the total fatty acid content in yolk, calculate the ratio that PUFAs in yolk ratio and yolk accounts for total fatty acids.Egg content of fatty acid made from this method is higher, and nutrition is higher.
Description
Technical field
The invention belongs to algae culture technical fields, specifically disclose one kind addition in chicken feed and are formulated microalgae to prepare
The method of high nutrition egg.
Background technique
Fatty acid is long aliphatic hydrocarbon chain, different types of fatty acid length, saturation degree and in terms of
All difference.Compared to saturated fatty acid and monounsaturated fatty acids, containing there are two and more than two unsaturated bonds more insatiable hungers
There is even more important physiological action to body with fatty acid (PUFAs).
PUFAs is broadly divided into ω -3 series fatty acid and ω -6 series fatty acid, wherein apart from hydrogen-oxygen chain carboxyl distalmost end
Double bond be ω -3 series fatty acid between 3rd, 4 carbon atom reciprocal, the double bond apart from hydrogen-oxygen chain carboxyl distalmost end exists
It is ω -6 series fatty acid between 6th, 7 carbon atom reciprocal.
Microalgae is the high-quality source of PUFAs, a variety of essential amino acids rich in needed by human body.In fact, people open already
Begin using PUFAs abundant in microalgae, such as in aquaculture, fish, shellfish and software are improved by manually adding microalgae
PUFAs (especially ω -3PUFAs) in animal.But there is not also research that microalgae is applied to chicken feed to improve egg now
The research of PUFAs.
Summary of the invention
The purpose of the present invention is to provide a kind of to add method of the formula microalgae to prepare high nutrition egg in chicken feed.
In order to achieve the above object, base case of the invention are as follows:
A method of addition formula microalgae is in chicken feed to prepare high nutrition egg, comprising the following steps:
(1) dry algae powder is made in algae strain；
(2) laying hen will be fed containing the mixed fodder of basal feed and algae powder and harvesting egg twice, records laying hen daily
Daily feed intake, laying rate, feedstuff-egg ratio and egg average egg weight；
(3) albumen of egg and yolk are separated, compares yolk color, measures the total fatty acid content in yolk, calculates
PUFAs accounts for the ratio of total fatty acids in yolk ratio and yolk.
The working principle and beneficial effect of this base case are:
The algae strain that the application will be enriched in fatty acid is applied to egg feedstuff, in the yolk color of obtained egg and high yolk
The ratio that PUFAs accounts for total fatty acids increases, i.e., the nutritive value using egg made from this method is higher.
Further, the basal feed is corn-soybean meal Basic drawing, and basal feed includes corn, dregs of beans, shell
Powder, soya-bean oil, fish meal and premix, corn and dregs of beans additive amount 2:1, conch meal 5%, soya-bean oil 3%, fish meal 2%, premix 3%.
Corn-soybean meal Basic drawing is cheap and easy to get.
Further, the algae strain is algae strain S7-M11, chlorella C4 and Euglena E8, the content of fatty acid of above-mentioned three seeds algaes strain
It is higher.
Further, the basal feed: mass ratio=95:5 of algae powder, using the egg that aforementioned proportion feeding laying hen is worth
Nutritive value it is preferable.
Further, yolk color made from step (3) improves 5-7 coloration, and for universal, egg yellow is in very great Cheng
Reflect the nutrition condition of chicken feed on degree, the deeper yolk nutritive value of color is higher.
Specific embodiment
Below by specific embodiment, the present invention is described in further detail.
Embodiment
Basic drawing in the application is corn-soybean meal Basic drawing, wherein corn and dregs of beans additive amount 2:1, shell
Powder 5%, soya-bean oil 3%, fish meal 2%, premix 3%.Experimental group word material is mixed for the corresponding microalgae of each group and the ratio of basal feed
Close diet.Diet is that professional feeding personnel purchase raw material, periodically carries out mixing and prepares diet.
Premix provides for every kilogram of diet: VA-8000IU, VB1-4mg, VB2- 3.6mg, VB5- 40mg, VB6- 4mg,
VB120.02mg, VD3- 3000IU, VE-20IU, D pantothenic acid -11mg, VK3- 20mg, biotin -0.15mg, folic acid -1.1mg resist
Oxidant -100mg, niacin -10mg, Cu-10mg, Fe-80mg, Mn-80mg, Zn-75mg, I-0.40mg, Se0.30mg.
In the application laying hen be Ross laying hen, 30 week old, 48, laying rate 85%.
Test three plants of microalgaes used and carry out sterile algae activation in laboratory first, then carry out it is sterile spread cultivation step by step, spread cultivation
100L raceway pond culture is entered the room after to 1L system, continues to cultivate into outdoor IOOOL raceway pond later, microalgae is in raceway pond
On-line monitoring in situ is carried out to pH, temperature when culture, progress absorbance detection works as cell to determine microalgae density within every two days
Quantity reaches 2 × 106A mL-1, when, microalgae recovery is carried out with disk plate centrifuge, algal gel is collected, dry algae powder is made, is protected from light, room temperature
It saves.
Testing program
(1) algae powder adding method
This experiment sets 4 groups altogether, and different algae powder is made an addition to basal feed and is made into different feeds progress laying hen feeding, specifically
Algae powder adds scheme such as the following table 1.
Table 1
| Grouping | Forage compounding |
| Control group | 100% basal feed |
| Embodiment 1 | 95% basal feed+5%S7-M11 algae powder |
| Embodiment 2 | The small algae powder C4 powder of 95% basal feed+5% |
| Embodiment 3 | + 5% Euglena E8 powder of 95% basal feed |
(2) laying hen is grouped
Ross laying hen 48 are selected similar in health, figure, is randomly divided into 4 groups, every group of 12 chickens.2 chickens of each chicken coop,
Chicken coop guarantees that every group of 6 chicken coops are dispersed in the different location of henhouse when discharging, and causes shadow to experiment to avoid position difference
It rings.Laying hen for every group is numbered, if 112 chickens of embodiment are No. Ml-12, embodiment 2 and embodiment 3 and so on.
(3) feeding management
Using enclosed breeding mode, two layers of placement of chicken coop point.The illumination side combined using artificial lighting with natural light
Formula, guarantees daily illumination 16h, and temperature maintains the circulation of air in 25-30 DEG C of range, relative humidity 50-90%, henhouse.Daily into
Row cloth twice, the morning and afternoon are each primary, guarantee that feed is sufficient, feed is dry powder-shaped.Every 5 days cleaning henhouses are primary, disinfection
Once, safe epidemic prevention is carried out.Pick up egg twice daily.Experiment carries out 30 days, and O-20 days each experimental group feeds are carried out by table 1, from 21
It starts, and each group feeds basal feed, and the change of egg quality after microalgae is not added in research.
(4) test assessment
1. performance in layers is assessed
Assessed by four daily feed intake, average egg weight, laying rate, feedstuff-egg ratio indexs Egg Production of Laying Hens situation whether by
Algae powder adds in feed and is changed.Concrete operation method is to record daily each group egg production, and weigh to egg, weekly
Clear material is primary.According to fore-telling column formula parameter:
Daily feed intake (g)=(addition forage volume-residual feed amount) ÷ feeding number of days；
Average egg weight=egg is total in the egg total weight ÷ statistics phase in the statistics phase；
Laying rate (%)=always raise number of days × 100 in the statistics phase in total egg number ÷ statistics phase；
Feed consumption rate (kg) the ÷ total yield egg size amount (kg) of feedstuff-egg ratio=in the statistics phase
2. egg quality measures
Egg 3 was selected at random in the 1 of experiment periods, 3,5,7,9,11,13,17,19,21,23,25,27,29,30 day each group
Piece, egg size is weighed, and separated albumen yolk with egg device, weighed respectively, calculates yolk ratio, protein ratio, with this to each
Group egg quality is assessed.Being scored with Roche colorimetric fan yolk color, (Roche colorimetric fan is divided into 1-15 coloration, is
It is specifically used to the tool assessed yolk color).
3. fatty acid component measures in yolk
2. dry powder is made by freeze drier in isolated yolk in, 500mg is weighed and carries out grease extraction, and will
The grease of proposition is dissolved in 1mL n-hexane, and 80uL is taken to carry out esterification and fatty acid component analysis every time,
Conclusion
1. after mixing, analyzing each group feed fat acid constituents, as a result such as the following table 2, the feed of three groups of addition microalgaes
Its PUFAs is apparently higher than control group, and wherein 1 effect of optimization of embodiment is the most obvious, PUFAs A wide selection of colours and designs in feed, content
It is abundant.
Table 2
| Fatty acid | Control group | Embodiment 1 (barrel mast) | Embodiment 2 (bead) | Embodiment 3 (Euglena) |
| C14:0 | ND | 0.36 | 0.22 | 0.45 |
| C16:0 | 16.06 | 17.71 | 16.43 | 16.87 |
| C16:1 | ND | 4.76 | 4.16 | 4.33 |
| C18:0 | 3.08 | 2.43 | 2.84 | 2.41 |
| C18:1 | 20.55 | 17.12 | 17.98 | 17.25 |
| C18:2 | 58.03 | 44.35 | 47.08 | 46.98 |
| C18:3 | 2.28 | 1.38 | 1.86 | 1.33 |
| C20:4 | ND | 1.92 | 0.34 | 0.52 |
| C20:5 | ND | 3.97 | 0.81 | 1.59 |
| C22:6 | ND | 0.42 | ND | ND |
2. algae strain S7-M11, chlorella C4 and Euglena ES are made an addition to chicken feed respectively to be tested.The result shows that three plants
Algae, which makes an addition to, does not influence performance in layers and egg quality in chicken feed, but yolk color can be improved 5-7 coloration,
And improve the ratio that PUFAs in yolk accounts for total fatty acids.Three kinds in examination algae strain, the effect of S7-Mll is to PUFAs in yolk
Raising effect it is the most obvious, 18.56% and 77.57% has been respectively increased in ω -6PUFAs and ω -3PUFAs in this group of yolk,
And alpha-linolenic acid, three kinds of important PUFAs of arachidonic acid and docosahexaenoic acid are improved.
What has been described above is only an embodiment of the present invention, and the common sense such as well known specific structure and characteristic are not made herein in scheme
Excessive description.It, without departing from the structure of the invention, can be with it should be pointed out that for those skilled in the art
Several modifications and improvements are made, these also should be considered as protection scope of the present invention, these all will not influence what the present invention was implemented
Effect and patent practicability.
Claims (5)
1. a kind of method of the addition formula microalgae to prepare high nutrition egg in chicken feed, which is characterized in that including following step
It is rapid:
(1) dry algae powder is made in algae strain；
(2) laying hen will be fed containing the mixed fodder of basal feed and algae powder and harvesting egg twice, records the every of laying hen daily
Daily ingestion amount, laying rate, feedstuff-egg ratio and egg average egg weight；
(3) albumen of egg and yolk are separated, compares yolk color, measures the total fatty acid content in yolk, calculates yolk
PUFAs accounts for the ratio of total fatty acids in ratio and yolk.
2. a kind of method of the addition formula microalgae to prepare high nutrition egg in chicken feed as described in claim 1, special
Sign is that the basal feed is corn-soybean meal Basic drawing, and basal feed includes corn, dregs of beans, conch meal, soya-bean oil, fish
Powder and premix, corn and dregs of beans additive amount 2:1, conch meal 5%, soya-bean oil 3%, fish meal 2%, premix 3%.
3. a kind of method of the addition formula microalgae to prepare high nutrition egg in chicken feed as claimed in claim 2, special
Sign is that the algae strain is algae strain S7-M11, chlorella C4 and Euglena E8.
4. a kind of method of the addition formula microalgae to prepare high nutrition egg in chicken feed as claimed in claim 3, special
Sign is, the basal feed: mass ratio=95:5 of algae powder.
5. a kind of method of the addition formula microalgae to prepare high nutrition egg in chicken feed as claimed in claim 4, special
Sign is that yolk color made from step (3) improves 5-7 coloration.
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| CN201811049778.6A CN109105636A (en) | 2018-09-10 | 2018-09-10 | A method of addition formula microalgae is in chicken feed to prepare high nutrition egg |
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| CN201811049778.6A CN109105636A (en) | 2018-09-10 | 2018-09-10 | A method of addition formula microalgae is in chicken feed to prepare high nutrition egg |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104757299A (en) * | 2015-03-20 | 2015-07-08 | 新奥科技发展有限公司 | Chicken feed containing microalgae |
| US20170202894A1 (en) * | 2014-07-17 | 2017-07-20 | Cornell University | Omega-3 fatty acid enrichment of poultry products with defatted microalgae animal feed |
| CN108308443A (en) * | 2017-01-18 | 2018-07-24 | 新奥科技发展有限公司 | A kind of feed addictive of layer chicken, egg feedstuff and preparation method thereof |
-
2018
- 2018-09-10 CN CN201811049778.6A patent/CN109105636A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170202894A1 (en) * | 2014-07-17 | 2017-07-20 | Cornell University | Omega-3 fatty acid enrichment of poultry products with defatted microalgae animal feed |
| CN104757299A (en) * | 2015-03-20 | 2015-07-08 | 新奥科技发展有限公司 | Chicken feed containing microalgae |
| CN108308443A (en) * | 2017-01-18 | 2018-07-24 | 新奥科技发展有限公司 | A kind of feed addictive of layer chicken, egg feedstuff and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李雨晨: "高产PUFAs微藻的筛选、营养胁迫及其在鸡饲料中应用的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
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