CN109097268A - A kind of spraying device and the method that cell prints inoculation by spraying is carried out using the device - Google Patents
A kind of spraying device and the method that cell prints inoculation by spraying is carried out using the device Download PDFInfo
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- CN109097268A CN109097268A CN201810759305.9A CN201810759305A CN109097268A CN 109097268 A CN109097268 A CN 109097268A CN 201810759305 A CN201810759305 A CN 201810759305A CN 109097268 A CN109097268 A CN 109097268A
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- 238000005507 spraying Methods 0.000 title claims abstract description 121
- 238000011081 inoculation Methods 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000007639 printing Methods 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims description 50
- 239000007921 spray Substances 0.000 claims description 34
- 238000009530 blood pressure measurement Methods 0.000 claims description 9
- 238000003854 Surface Print Methods 0.000 claims description 8
- 238000012544 monitoring process Methods 0.000 claims description 3
- 230000001464 adherent effect Effects 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 72
- 239000007789 gas Substances 0.000 description 31
- 210000001519 tissue Anatomy 0.000 description 10
- 239000006285 cell suspension Substances 0.000 description 9
- 210000004204 blood vessel Anatomy 0.000 description 8
- 238000010146 3D printing Methods 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 239000010410 layer Substances 0.000 description 5
- 238000011160 research Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 210000002889 endothelial cell Anatomy 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 2
- 230000002490 cerebral effect Effects 0.000 description 2
- 210000004087 cornea Anatomy 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 210000000626 ureter Anatomy 0.000 description 2
- 230000002485 urinary effect Effects 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000003592 biomimetic effect Effects 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010041 electrostatic spinning Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000004789 organ system Anatomy 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The invention discloses a kind of spraying devices for cell inoculation, including spraying system and rotatable stage；The rotatable stage is used to form cell and is sprayed for carrying bio-carrier, the spraying system, carries out cell to the bio-carrier on the rotatable stage and prints inoculation by spraying；The rotatable stage includes carrier bracket and motor, and the motor can control the carrier bracket and carry out uniform motion.The method that cell prints inoculation by spraying is carried out using above-mentioned spraying device the invention also discloses a kind of.The spraying device of cell inoculation provided by the present invention may be implemented to carry out cell inoculation in the surfaces externally and internally of biological duct structure and curved-surface structure, it is uniformly distributed inoculating cell in biological duct structure and curvature structure, promote it adherent rapidly and growth, and carry out cell inoculation by way of spraying printing and can be used for the specificity layering inoculation of a variety of cells, the organizational project building of biological duct and curvature structure can be fast implemented.
Description
Technical field
The present invention relates to field of tissue engineering technology, and in particular to a kind of for the spraying device of cell inoculation and using the device
Carry out the method that cell prints inoculation by spraying.
Background technique
The core of organizational project is exactly to establish the three-dimensional space complex of cell and biomaterial, that is, has vital work
Body tissue to the reconstruction to disease damage tissue progress form, structure and function and reaches permanent substitution.However, carrying out group
When the building of weaver's journey, cell how to be made to be uniformly distributed and realize the specificity of various kinds of cell in histoorgan of different shapes
Layering is constructed so that a urgent need overcomes the problems, such as, such as tends to the institutional framework table of plane relative to skin tissue engineering etc.
For the cell inoculation in face, curvature or pipeline configuration, cornea, tracheae, oesophagus, urinary catheter, ureter, blood vessel etc., it is inside and outside
Superficial cell is when carrying out cell inoculation due to its structural specificity and gravity uniformly inoculation and various kinds of cell difficult to realize
Specificity layering building.
It realizes that celliferous tubular structure manufacture has had more document report using 3D printing method at present, prints
Method includes using ink jet type, laser excitation formula and extruded type.It can be realized using the cell printing based on ink-jet, laser direct-writing
The controllable deposition of many cells.It is logical for constructing the nutrition of entity structure based on the tubular structure main purpose for squeezing out principle construction
Road, dynamically load needed for cannot achieve blood vessel Exploration of Mechanism.
True blood vessel structure is made of endothelial cell, smooth muscle cell and fibroblast, existing literature report
The blood vessel structure that 3D printing method produces is largely simple gel structure, and intensity is unable to satisfy Subsequent vessel cyto-mechanics
Research.Though the current histoorgan biomimetic features containing channel, which have using pipeline configuration, simulates showing for intracorporal complicated blood vessel
Example, but these pipelines are all difficult to form controllable single-layer or multi-layer cell layer structure, Tsinghua University's organ system in microcosmic point
It makes center and successfully prepares vascularized organ's precursor with channel using 3D printing technique and rotation combination die methods, but is existing
The perfusion and field planting of cell cannot be technically carried out on tubular structure inner wall.In order to solve this problem, many research institutions
A series of technique study has been carried out thus, such as application No. is 201410535181.8 Chinese invention patents to utilize more spray heads
Based on 3D printing method combines more inner core rotation combination die methods, using the effect of extra electric field, control on a microscopic scale micro-
It the attachment of cell monolayer and aligns in the pipeline of fluid, the bionical knot more close to tissue or organ is prepared with this
Structure.However, since vigor and function tool of the electric field force to cell has a certain impact, and be only applicable to pipeline configuration and be not suitable for
In the organizational project building of the curvature structure of non-pipeline, to limit its use.
The research group of Wyss research institute of Harvard University carries out 3D printing and contains vascular tissue, will contain extracellular matrix
The special marking ink of (gelatin-Fibrin Glue) and living cells is printed upon 3D by scheduled location and shape size with filiform and beats
It prints in tissue, since the state of the liquid-solid of gelatin is temperature controlled, it is possible thereby to control marking ink by temperature change
For the solid-state ((70-95 DEG C) of gel state (4 DEG C) and different hardness.In this way in the tissue of printing, where special marking ink
Position just will form luminal structure, then endothelial cell be injected in lumen position again, so that cell be made to grow again in lumen position
At blood vessel structure, however the endothelial cell injected in this way being unevenly distributed in lumen, so as to cause its function
Can excalation, thus cannot normal blood vessel structure in simulation normal human very well.
The prior art also discloses the cerebral microvascular mould of a kind of combination 3D biometric print and the manufacture of electrostatic spinning complex technique
Type, the program solve subproblem of the existing technology to a certain extent, however because its is complicated for operation, technical requirements
Height, and it is limited only to the organizational project building of cerebral microvascular model, and such as cornea, tracheae, oesophagus, urinary catheter, ureter, blood vessel
The layering Construct question of the cellular system engineering of the surfaces externally and internally of equal tissues not yet can be well solved.
Summary of the invention
To overcome existing technology at least one of which defect, the present invention provides a kind of sides using cell spray inoculation
Method carries out the spraying device of cell inoculation, not only may be implemented uniformly to be inoculated with high vigor cell, can also form controllable single layer
Or multi-layer cellular layer structure, realize the specificity layering inoculation of various kinds of cell.
To achieve the purpose of the present invention, it is achieved using following technical scheme:
A kind of spraying device for cell inoculation, including spraying system and rotatable stage；The rotatable stage is for holding
Bio-carrier is carried, the spraying system is used to form cell and is sprayed, and carries out cell to the bio-carrier on the rotatable stage
Spraying printing inoculation；The rotatable stage includes carrier bracket and motor, and the motor can control the carrier bracket and carry out
Uniform motion.
The present invention provides a kind of spraying devices that cell inoculation is carried out to bio-carrier, are obtained uniformly by spraying system
Cell it is spraying so that the spraying print surface for being uniformly attached to bio-carrier of cell.The present invention is using the side printed by spraying
Formula can print out the cell with high activity, and since cell is evenly distributed on bio-carrier, and printing cell is promoted to paste rapidly
Wall, growth.Cell inoculation is carried out by way of spraying printing, only needs to change cell suspension used by spraying system, and
Routine culture is carried out after printing every time, next layer is being printed after cell is securely adherent, suitable for the special of various kinds of cell
Property layering inoculation, can fast implement bio-carrier organizational project building.
In a wherein embodiment, the spraying system includes liquid control device, fog-spray nozzle, pressure monitoring and spray
Mist control device；The liquid control device includes liquid bogey and mobile band, squeezes liquid bogey by mobile band
Liquid is squeezed out；The fog-spray nozzle includes liquid supplying passage and gas delivery channels, the liquid supplying passage and the liquid
The connection of body bogey, the liquid that the liquid bogey squeezes out flow into the liquid supplying passage；The pressure detecting and
Spraying control device includes by the sequentially connected air pump of pipeline, gas pressure and spray pattern adjuster, gas pressure measurement
Table, the pipeline for being finally connected with gas pressure measurement table is connect with the gas delivery channels, and gas is passed through the gas
Transfer passage.
Preferably, the fog-spray nozzle is binary channels fog-spray nozzle, including liquid supplying passage and gas delivery channels, liquid is defeated
It send and inputs cell suspension in channel, gas delivery channels input compressed gas, and cell suspension and compressed gas are mixed at fog-spray nozzle
When conjunction, compressed air and cell suspension interact, and cell suspension is atomized and is sprayed.
In a wherein embodiment, the rotatable stage is at least partly detachable.Carrying out life of different shapes
The carrier bracket of different shape and size may be selected when the spraying printing of object carrier.
Specifically, spraying device provided by the present invention mainly for bio-carrier be biological duct structure and curved surface knot
Structure.
In a wherein embodiment, when the cell for carrying out biological duct structural outer surface prints inoculation by spraying, institute
Stating rotatable stage includes the carrier bracket that the tubulose on its surface is placed on for bio-carrier, and the carrier bracket and the motor connect
It connects, and the motor can control the carrier bracket and at the uniform velocity rotate around its axial direction.
In a wherein embodiment, when the cell for carrying out biological duct structure interior surface prints inoculation by spraying, institute
Stating rotatable stage includes first motor and the second motor；The first motor is connected with drag hook bracket, on the drag hook bracket
Equipped at least two first drag hooks, the first motor can drive the drag hook support level to move；The second motor connection
The carrier bracket, the end of the carrier bracket are equipped with the second drag hook, second motor can control the carrier bracket around
Its axial direction at the uniform velocity rotates.
In a wherein embodiment, when the cell for carrying out curved-surface structure prints inoculation by spraying, the carrier bracket
Including curved surface, the curved surface can control the carrier bracket at the uniform velocity left and right for carrying curved bio-carrier, the motor
It swings.
A method of cell is carried out using above-mentioned spraying device and prints inoculation by spraying, bio-carrier is placed in the carrier
On bracket, the carrier bracket as described in the motor control carries out uniform motion, while spraying system is prepared cell and is sprayed, so that
Cell is attached to the surface of bio-carrier by spraying.
When bio-carrier is biological duct, and the cell of progress biological duct outer surface prints inoculation by spraying, specific steps
It is as follows:
S11: using the carrier bracket of tubulose, biological duct is placed on to the outer surface of the carrier bracket；
S12: opening motor and spraying system, drives the carrier bracket at the uniform velocity to rotate around its axial direction by the motor, together
Shi Suoshu spraying system sprays cell and is sprayed, so that the spraying outer surface for being equably attached to biological duct of cell.
When bio-carrier is biological duct, and the cell of progress biological duct inner surface prints inoculation by spraying, specific steps
It is as follows:
S21: an end of biological duct being sutured, which is received to pipeline, and the end is installed on described
On second drag hook, then hook with the first drag hook the open end of biological duct；
S22: opening first motor, draws suture end motion of the drag hook bracket to biological duct from first motor, makes biological duct
It realizes and flips outward, close first motor；
S23: opening the second motor and spraying system, drives the carrier bracket at the uniform velocity to revolve around its axial direction by the second motor
Turn, while the spraying system sprays cell and is sprayed, so that the spraying inner surface for being equably attached to biological duct of cell.
Preferably, in step S23, when the second motor drives the carrier bracket at the uniform velocity to rotate around its axial direction, the
One motor is also with the second motor constant speed rotation, so that carrier bracket is uniformly around axial-rotation.
When bio-carrier be curved-surface structure, carry out curved-surface structure cell print by spraying be inoculated with when, the specific steps are as follows:
S31: selection and carrier bracket similar in bio-carrier curvature are installed on motor, bio-carrier are then installed to load
On body support frame；
S32: motor and spraying system are opened, motor control carrier bracket carries out at the uniform velocity alternatively swinging, while spraying system sprays
Cell is spraying, so that the spraying surface for being equably attached to bio-carrier of cell.
Compared with the prior art, it is carried out the present invention provides a kind of spraying device for cell inoculation and using the device
The method that cell prints inoculation by spraying, has the advantages that
(1) there is high activity by the cell that spraying printing type prints.
(2) spraying device of cell inoculation provided by the present invention may be implemented in biological duct structure and curved-surface structure
Surfaces externally and internally carries out cell inoculation, is uniformly distributed inoculating cell in biological duct structure and curvature structure, promotes it rapidly
Adherent and growth, and carry out cell inoculation by way of spraying printing and can be used for the specificity layering inoculation of a variety of cells,
The organizational project building of biological duct and curvature structure can be fast implemented.
(3) present invention can overturn biological duct structure, allow to carry out the tissue of biological duct structure interior surface
Engineering building becomes easier to.
(4) it is changeable to be sprayed printing model to cell of the invention, may be implemented to continue spraying printing and fixed time interval is beaten by spraying
Print or manually control spraying printing.
(5) carrier bracket of the invention can be replaced, and operator can replace suitable size, suitable shape according to self-demand
Carrier bracket, operation is simple.
Detailed description of the invention
Fig. 1 is the total figure that entire cell is sprayed print system.
Wherein, in Fig. 18 be simulation carry out biological duct inner face cell print by spraying be inoculated with when it is related.
Fig. 2 is that the liquid in spraying system carries instrument and fog-spray nozzle.
Fig. 3 is for carrying out spraying rotatable stage to biological duct lateral surface.
Fig. 4 is for carrying out spraying rotatable stage to biological duct medial surface.
Fig. 5 is for carrying out spraying rotatable stage to curved-surface structure.
Description of symbols: 1, mobile band；2, gas pressure measurement table；3, computer；4, pressure detecting and spraying control dress
It sets；5, air pump；6, plastic plate；7, liquid bogey；8, rotatable stage；9, fog-spray nozzle；801, first motor；802, second
Motor；803, the first drag hook；804, pipeline sutures closed end；805, the second drag hook；806, bio-carrier；807, carrier bracket；
901, fluid outlet channels；902, gas output channel；903, binary channels fog-spray nozzle.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, with reference to the accompanying drawing to embodiment of the present invention
It is described in more detail.
Embodiment
As shown in Figure 1, present embodiments providing a kind of being sprayed for method progress cell inoculation using cell spray inoculation
Device, including spraying system and rotatable stage.
Wherein, spraying system includes liquid control device, fog-spray nozzle 9, pressure monitoring and spraying control device；The liquid
Control device includes liquid bogey 7 and mobile band 1, and squeezing liquid bogey 7 by mobile band 1 will be in liquid bogey
Liquid squeeze out；The fog-spray nozzle 9 includes liquid supplying passage 901, gas delivery channels 902 and binary channels fog-spray nozzle 903, institute
It states liquid supplying passage 901 to connect with the liquid bogey 7, described in the liquid inflow that the liquid bogey 7 squeezes out
Liquid supplying passage 901, the gas of the liquids and gases transfer passage 902 of liquid supplying passage 901 is in binary channels fog-spray nozzle 903
When place's mixing, compressed gas and liquid interact, and cell suspension is atomized and is sprayed；The pressure detecting and spraying control dress
Setting 4 includes by the sequentially connected air pump 5 of pipeline, gas pressure and spray pattern adjuster 4, gas pressure measurement table 2, finally
The pipeline for being connected with gas pressure measurement table 2 is connect with the gas delivery channels 902, and gas is passed through the gas and is conveyed
Channel 902.
Embodiment as one preferred, on the basis of the above embodiments, spraying device further include computer 3, computer 3
It is connected respectively with mobile band 1, first motor 801, the control of the second motor 802.
Embodiment as one preferred, on the basis of the above embodiments, mobile band 1 and liquid bogey 7 it
Between can increase by one piece of plastic plate 6, mobile band 1 can be improved to the uniform force application degree of liquid bogey 7 in plastic plate 6.It moves
For dynamic band 1 by mobile with constant speed, promotion plastic plate 6 squeezes liquid bogey 7 with this.
Embodiment as one preferred, on the basis of the above embodiments, the members of rotatable stage are can
With disassembly, such as carrier bracket 807 as shown in Fig. 3 ~ 5, it can when carrying out the spraying printing of bio-carrier of different shapes
Select the carrier bracket of different shape and size.
When the cell for carrying out biological duct structural outer surface prints inoculation by spraying, used carrier bracket 807 is pipe
Shape carrier bracket, as shown in figure 3, the working end of the second motor 802 is connect with carrier bracket 807.Need to be to biological duct structure
Bio-carrier outer surface carry out cell spray inoculation when, bio-carrier is placed on the outer surface of carrier bracket 807, carrier bracket
807 are installed on the second motor 802, can have 802 control vector bracket 807 of the second motor axially at the uniform velocity to rotate around it.
The cell of the outer surface of progress biological duct structure prints inoculation by spraying, and the specific method is as follows: before the printing,
Firstly, selection is close and smaller with required progress biological duct (the i.e. bio-carrier 806) inside diameter of cell spray inoculation
The tubular carrier bracket 807 of size, attaches it in objective table, and biological duct is placed on carrier bracket 807.Then, will
The cell suspension gathered is fitted into the liquid bogey 7 being connected with spray head, sets the corresponding operation ginseng in computer 3
Number opens gas pressure measurement table 2, gas pressure and spraying control device 4, air pump 5 and the second motor 802, so that mobile band 1
It is moved by constant speed, equably squeezes liquid bogey 7, realized the constant speed flowing of liquid, observe gas pressure measurement
Barometer reading in table 2, regulating gas pressure and spray pattern adjuster 4 adjust suitable air pressure, and gas and liquid is made to exist
Fog-spray nozzle exit crosses, so that it is spraying to form soft cell.In addition, equally may be used by the correspondence parameter in setting computer 3
It rotates the second motor 802 control bio-carrier 806 counterclockwise according to a constant and slow speed, is carried out with this
The cell of biological duct structural outer surface prints by spraying.It is actually being sprayed in print procedure, if wanting to switch different spraying printings
Mode also can need regulating gas pressure and spraying control device 4 and change spraying frequency and continue according in practical operation
Time.
When the cell for carrying out biological duct inner face prints inoculation by spraying, used rotatable stage 8 is on the basis Fig. 3
On, the second drag hook 804 is increased in the end of carrier bracket 807, while also adding first motor 801, first electrode 801 connects
It is connected to drag hook bracket, two the first drag hooks 803 are at least connected on drag hook bracket.
The cell for carrying out biological duct inner face prints inoculation by spraying method particularly includes: firstly the need of by the inner face of biological duct
It flips outward, specific method may is that as shown in figure 4, being sutured pipeline one end to obtain pipeline suture closed end first
804, and pipeline is sutured in closed end 804 and is received to pipeline；Then biological duct is mounted on the second drag hook 805
On tubular carrier bracket 807, pipeline suture closed end 804 is hooked by the second drag hook 805, while hooking it with the first drag hook 803
First motor 801 is finally opened in open end, so that the first drag hook 803 slowly closes the open end of biological duct to pipeline suture
The direction drawing (appear as from Fig. 4 and pull to the left) for closing end 804, so realizes its pipeline inner face and flips outward.Then pass through
Spraying printing type described above carries out spraying printing to the inner surface of pipeline overturn, then carries out routine culture, to
Its securely it is adherent after its inner surface is overturn to insides of pipes face by same mode again.
When carry out curvature structure cell by spraying print be inoculated with when, used carrier bracket 807 be include a curved surface
Bracket, as shown in figure 5, the curved surface of bio-carrier 806 carrying and carrier bracket 807.
In order to realize that the cell of curvature structure prints by spraying, selected first before printing as shown in Figure 5 with bio-carrier curvature
Similar carrier bracket, and attach it on the second motor 802.With the cell of the biological duct structural outer surface side of printing by spraying
Method is similar, and first the cell suspension being collected into is added in liquid bogey 7, is joined by the operation that computer 3 sets mobile band 1
Number, push it to plastic plate 6 according to certain speed, with this come realize to liquid bogey 7 it is constant slowly
It pushes, realizes that the constant speed of cell suspension squeezes out.The parameter of pressure detecting and spraying control device 4 can be adjusted as needed simultaneously,
The gas pressure of printing is changed with this, if wanting to change spray pattern, can also pass through 4 gas of pressure detecting and spraying control device
Pressure and spray pattern adjuster are realized.In order to realize that uniform curvature is spraying, we can be by 3 computers to instrument 802
Motor carry out parameter setting, thus change 807 carrier brackets (curvature shape) motion mode be rotate clockwise 90 ° with counterclockwise
It is rotated by 90 ° alternately, to realize the spraying printing of curvature structure.
The above embodiment is merely an example for clearly illustrating the present invention, and is not to embodiment party of the invention
The restriction of formula.For those of ordinary skill in the art, other differences can also be made on the basis of the above description
The variation or variation of form.There is no necessity and possibility to exhaust all the enbodiments.It is all in spirit and original of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the scope of protection of the claims of the present invention within then.
Claims (10)
1. a kind of spraying device for cell inoculation, which is characterized in that including spraying system and rotatable stage；The rotation
Objective table is for carrying bio-carrier, and the spraying system is used to form cell and is sprayed, to the biology on the rotatable stage
Carrier carries out cell and prints inoculation by spraying；The rotatable stage includes carrier bracket and motor, and the motor can control described
Carrier bracket carries out uniform motion.
2. the spraying device according to claim 1 for cell inoculation, which is characterized in that the spraying system includes liquid
Member control apparatus, fog-spray nozzle, pressure monitoring and spraying control device；
The liquid control device includes liquid bogey and mobile band, squeezes liquid bogey by mobile band and squeezes liquid
Out；
The fog-spray nozzle includes liquid supplying passage and gas delivery channels, and the liquid supplying passage and liquid carrying fill
Connection is set, the liquid that the liquid bogey squeezes out flows into the liquid supplying passage；
The pressure detecting and spraying control device include passing through the sequentially connected air pump of pipeline, gas pressure and spray pattern tune
Device, gas pressure measurement table are saved, the pipeline for being finally connected with gas pressure measurement table is connect with the gas delivery channels, and will
Gas is passed through the gas delivery channels.
3. the spraying device according to claim 1 for cell inoculation, which is characterized in that the rotatable stage is at least
Part is detachable.
4. the spraying device according to claim 1 or 2 or 3 for cell inoculation, which is characterized in that the rotation loading
Platform includes the carrier bracket that the tubulose on its surface is placed on for bio-carrier, and the carrier bracket is connect with the motor, and described
Motor can control the carrier bracket and at the uniform velocity rotate around its axial direction.
5. the spraying device according to claim 1 or 2 or 3 for cell inoculation, which is characterized in that the rotation loading
Platform includes first motor and the second motor；The first motor is connected with drag hook bracket, and the drag hook bracket is equipped at least two
A first drag hook, the first motor can drive the drag hook support level to move；Second motor connects the carrier branch
The end of frame, the carrier bracket is equipped with the second drag hook, and second motor can control the carrier bracket around its axial direction
At the uniform velocity rotate.
6. the spraying device according to claim 1 or 2 or 3 for cell inoculation, which is characterized in that the carrier bracket
Including curved surface, the curved surface can control the carrier bracket at the uniform velocity left and right for carrying curved bio-carrier, the motor
It swings.
7. a kind of carry out the method that cell prints inoculation by spraying using described in any item spraying devices according to claim 1 ~ 6,
It is characterized in that, bio-carrier is placed on the carrier bracket, the carrier bracket as described in the motor control is at the uniform velocity transported
It is dynamic, while spraying system is prepared cell and is sprayed, so that cell is attached to the surface of bio-carrier by spraying.
8. the method according to claim 7 for carrying out cell and printing inoculation by spraying, which is characterized in that when bio-carrier is made a living
When the cell of conduit, progress biological duct outer surface prints inoculation by spraying, the specific steps are as follows:
S11: using the carrier bracket of tubulose, biological duct is placed on to the outer surface of the carrier bracket；
S12: opening motor and spraying system, drives the carrier bracket at the uniform velocity to rotate around its axial direction by the motor, together
Shi Suoshu spraying system sprays cell and is sprayed, so that the spraying outer surface for being equably attached to biological duct of cell.
9. the method according to claim 7 for carrying out cell and printing inoculation by spraying, which is characterized in that when bio-carrier is made a living
When the cell of conduit, progress biological duct inner surface prints inoculation by spraying, the specific steps are as follows:
S21: an end of biological duct being sutured, which is received to pipeline, and the end is installed on described
On second drag hook, then hook with the first drag hook the open end of biological duct；
S22: opening first motor, draws suture end motion of the drag hook bracket to biological duct from first motor, makes biological duct
It realizes and flips outward, close first motor；
S23: opening the second motor and spraying system, drives the carrier bracket at the uniform velocity to revolve around its axial direction by the second motor
Turn, while the spraying system sprays cell and is sprayed, so that the spraying inner surface for being equably attached to biological duct of cell.
10. the method according to claim 7 for carrying out cell and printing inoculation by spraying, which is characterized in that when bio-carrier is
Curved-surface structure, carry out curved-surface structure cell print by spraying inoculation when, the specific steps are as follows:
S31: selection and carrier bracket similar in bio-carrier curvature are installed on motor, bio-carrier are then installed to load
On body support frame；
S32: motor and spraying system are opened, motor control carrier bracket carries out at the uniform velocity alternatively swinging, while spraying system sprays
Cell is spraying, so that the spraying surface for being equably attached to bio-carrier of cell.
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| CN201810759305.9A CN109097268A (en) | 2018-07-11 | 2018-07-11 | A kind of spraying device and the method that cell prints inoculation by spraying is carried out using the device |
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