CN109090340A - A method of production animal feed is considered to be worth doing using reed - Google Patents
A method of production animal feed is considered to be worth doing using reed Download PDFInfo
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- CN109090340A CN109090340A CN201810912662.4A CN201810912662A CN109090340A CN 109090340 A CN109090340 A CN 109090340A CN 201810912662 A CN201810912662 A CN 201810912662A CN 109090340 A CN109090340 A CN 109090340A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention relates to a kind of methods for considering production animal feed to be worth doing using reed, specifically considered to be worth doing using the waste reed that reed pulp factory material papering section generates, through hydrothermal pretreatment, zytase and cellulose hydrolyzation, saccharomycetes to make fermentation, a kind of method for preparing yeast feed rich in xylo-oligosaccharide (XOS).This method is not only to have nutrition health-care functions to growth of animal the hydrolysis of hemicellulose in waste reed bits, it can replace the XOS product that conventional antibiotic uses, and cellulose is converted into fodder yeast, improve the concentration or content of XOS, it is a kind of protein i.e. rich in, amino acid, vitamin, it is rich in the health care type feedstuff of functionality XOS again, realizes the higher value application of waste reed bits.
Description
Technical field
The invention belongs to feed additive fields, and waste material (reed bits) are specifically left unused by the pre- water of high temperature in paper mill
Solution, Enzymatic hydrolysis, fermentation process, preparation contains abundant protein, amino acid, vitamin, and is rich in the health care of functionality XOS
The method of type feed.
Background technique
Reed is that one kind has extensive adaptation, resistance, the strong perennial grass family of fertility for growing environment
Plant, natural Phragmites communis population are mainly updated with the breeding of rhizome, are especially typical clonal line, growth cycle
It is short, low, yield height is required grown place, and application value is very big；With adsorbing pollution noxious material in water, purifying water, tie up
The effect of Wetland ecological is protected, the attention with the whole world to ecological environmental protection, the plantation of reed is also gradually by Chinese Government
It supports and protects.In recent years, reed wetland construction was carried out in China energetically, adhered to improving reed per unit area yield and total output, so that I
State's area of Phragmites communis is up to more than 50 ten thousand hm2, reed annual output 2500000~3,000,000 t.In one's early years, reed be mainly used for weaving a straw mat or other
Manual product as people contain the understanding of higher fiber cellulose content to reed, and is gradually available for pulping and paper-making, therefore, with reed
Reed substitutes wood raw material and carries out pulping and paper-making, not only can solve current timber resource shortage problem, also promotes paper industry
Development.But handle the slurry factory of 100,000 tons of reeds year, 10,000 tons of reed bits, main component are generated in the big appointment of bolting house
It is the clast and dust of reed film, reed leaf and some reeds, it must not be not as garbage disposal because these clasts are not suitable for papermaking
Fall, disposed generally in a manner of burning and bury, causes the serious wasting of resources.It is analyzed according to material composition, in these wastes
Containing a large amount of hemicellulose, cellulose, lignin, and this every kind ingredient can be environment friend by biorefining technical transform
Good type product is of great significance to the transition and upgrade for promoting paper mill.
XOS (xylo-oligosaccharide) is that one kind is led to by 2~7 xylose molecules as a kind of high value added product of hemicellulose
The functional oligose for crossing β-(Isosorbide-5-Nitrae) glycosidic bond links composition is the dietary fiber for being not easy to be digested degradation by alimentary canal, has choosing
The breeding of selecting property promotion Bifidobacterium, especially bifidobacterium adolescentis；Enhance absorption of the enteron aisle to calcium, vitamin；Help the gallbladder that disappears solid
Alcohol, grease；Immunity and other effects is improved, has been used widely in industries such as food, medicines and health protection, animal feeds.With
People to high-quality live it is continuous pursue, XOS healthcare function it is increasingly significant, demand goes up year by year, main at present logical
The hydrolysis of hemicellulose for crossing corncob is made, and raw material is relatively single.The XOS manufacturer represented with Shandong Long Liwei is also close several
Year throughout all parts of the country have been arrived, the motive force of this social demand and scientific and technological progress makes the reduction of production technology cost, product
The raising of purity and development and utilization new raw material become the research hotspot and bottleneck of the sector.
It is well known that biomass material component is headed by content of cellulose, numerous studies by being pre-processed to biomass, enzyme
Hydrolysis carrys out degraded cellulose into reducing sugar, and utilization is mainly the most mature with the production technology of bio-ethanol, but in raw material
Hydrolysis of hemicellulose product wood-sugar fermentation conversion ratio is low, it is difficult to utilize.Hemicellulose mainly with produce XOS, xylose, xylitol,
Based on the xyloses series of products such as furfural, the problem is that, during zytase-cellulose hydrolyzation is used in combination,
Due to the mutual package action of cellulose and hemicellulose, the thick xylanase hydrolysis containing a small amount of cellulase, so that half fiber
Dimension element while hydrolysis, also degraded, catabolite glucose by considerable part cellulose, not only has suppression to biological enzyme
Production is used, and also the purifying for subsequent XOS improves cost.If can make convert glucose at other with the same of value-added product
When, make XOS content ratio obtain improving relatively, can prepare one kind can be by balance host animal intestinal flora to animal
The active probiotic animal feed of Beneficial Effect is generated, correlative study shows by supplementing the ingredient, micro- in body gastrointestinal tract
The available adjustment of the ecological balance or maintenance supplement enteron aisle on number or type to promote the breeding of profitable strain in enteron aisle
The effects of necessary probiotics, realization dominant microflora, biological antagonist, effective substitute antibiotics, production and sale for livestock products
With two-win effect.
The amount of dissolution of target product is not only related with preparation process, and the selection of raw material is also particularly important.Reed is considered to be worth doing main
Ingredient is the clast and dust of reed film, reed leaf and some reeds, has done feedstock analysis to the raw material according to the method for NREL, discovery
Hemicellulose level (cellulose: hemicellulose: lignin=1.6:1.4:1) is higher, can be used as the raw materials for production of function XOS.
It is glucose that cellulose degradation inevitably occurs in the process for the produced in conventional processes for preparing XOS, and is produced
Glucose and xylan be difficult to separate, the purity and value of XOS product are affected, so this patent is innovatively by pre-hydrolyzed solution
With the glucose utilization saccharomycetes to make fermentation in enzyme hydrolyzate be rich in have vitamin B bioprotein (animal feed it is main at
Point), XOS content and purity as feed healthy ingredient are all improved significantly, and have simply implemented the high level of reed bits
Change and utilizes.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for preparing the yeast feed rich in xylo-oligosaccharide (XOS), opposite
Under mild treatment conditions, purity and all higher XOS product of yield, in order to achieve the object of the present invention, institute of the present invention are obtained
The lignocellulose raw material stated is reed bits, utilizes the side of hydrothermal pretreatment, zytase and cellulose hydrolyzation, saccharomycetes to make fermentation
Method, specifically includes the following steps:
1) reed bits are added to the water after carrying out hydrothermal pretreatment, it is cooling, it is separated by solid-liquid separation.Liquid obtained in it is
Pre-hydrolyzed solution.
2) zytase is added into solid obtained in step 1) and cellulase is hydrolyzed, saccharomycetes to make fermentation obtains
Obtain the animal feed rich in XOS.It is enzyme hydrolyzate that the liquid obtained after enzyme hydrolysis (enzymatic hydrolysis), which is wherein added,.
According to technical solution above, in preferred situation, reed bits raw material used is the production of reed slurry factory slurrying material papering section
Raw waste obtains the mixed waste based on reed stalk, reed film and trifling blade by impurity elimination, is clast state.
Reed bits used are the waste of reed slurry factory, utilize method there are no effective so far, and reed bits are that reed pulp is stocked up
The clast that process generates, is not required to additional cutting or pulverization process.
According to technical solution above, in preferred situation, the method for the reed bits raw material impurity elimination is to separate through 50 meshes,
Remove the dust of small particle.
According to technical solution above, in preferred situation, the condition of hydrothermal pretreatment described in step 1) are as follows: liquid-solid ratio
(volume: quality) is 10:1-4:1, and temperature is 140-190 DEG C, and the processing time is 20-60min.
According to technical solution above, in preferred situation, the saccharomycetes to make fermentation is the aerobic fermentation of saccharomycete.
According to technical solution above, in preferred situation, in step 2) zytase dosage be 100-600IU/g substrate,
Fiber enzyme dosage is 10-50FPU/g substrate, and hydrolysis substrate mass percent concentration is 2%-10%.
According to technical solution above, in preferred situation, enzyme hydrolysis process can be zytase and cellulase substep
Or synchronous hydrolysis, zytase and cellulase fractional hydrolysis process are as follows: first xylanase hydrolysis 8-15h, rear cellulase
Hydrolysis adds up hydrolysis 30-72h；Zytase and cellulase synchronous hydrolysis process are as follows: zytase and cellulase add simultaneously
Enter synchronous be added and hydrolyzes 30-72h.
According to technical solution above, in preferred situation, step 2) is that will obtain in step 1) when carrying out saccharomycetes to make fermentation
To liquid and step 2) in the obtained liquid of hydrolysis mix, as culture medium, the culture medium also needs additional 0.5-
The nitrogen source of 20% (w/v), the KH of 0.5-2% (w/v)2PO4, the MgSO of 0.5-1.5% (w/v)4。
According to technical solution above, in preferred situation, hydrolysis described in step 2) and fermentation (cellulose hydrolyzation and ferment
The fermentation of female bacterium) substep is when carrying out, and it is liquid (the enzyme water to obtain liquid obtained in step 1) (pre-hydrolyzed solution) and step 2)
Solve liquid) it is mixed by the volume ratio of 3:2-1:4, the fermentation of saccharomycete is carried out as culture medium, is obtained the animal rich in XOS and is raised
Material.
According to technical solution above, in preferred situation, hydrolysis described in step 2) and fermentation (cellulose hydrolyzation and ferment
The fermentation of female bacterium) it is synchronous when carrying out, it is by liquid obtained in step 1) (pre-hydrolyzed solution) and to solid obtained in step 2)
The xylanase hydrolysis liquid that middle first addition zytase is hydrolyzed is mixed by the volume ratio of 3:1-1:2, as training
Support base, add cellulase be hydrolyzed, saccharomycetes to make fermentation, occur that cellulose hydrolyzation simultaneously with saccharomycetes to make fermentation, obtain
Obtain the animal feed rich in XOS.
According to technical solution above, in preferred situation, the nitrogen source is urea or yeast extract.
According to technical solution above, in preferred situation, the saccharomycete can be pichia stipitis (Pichia
Stipitis), trichosporon cutaneum (Trichosporonsp) or saccharomyces cerevisiae, inoculum concentration are 3-10% (v/v).
According to technical solution above, in preferred situation, hydrolysis can substep or synchronous progress with fermentation in step 2).
According to technical solution above, in preferred situation, hydrolysis described in step 2) and fermentation (cellulose hydrolyzation and ferment
Female bacterium fermentation) substep progress, specifically: zytase is first added into solid obtained in step 1) and cellulase carries out water
Solution, obtains the mixed liquor of xylan and glucose, then carry out saccharomycetes to make fermentation, obtains the animal feed for being rich in XOS；Or
Zytase is first added into solid obtained in step 1) to be hydrolyzed, add cellulase and be hydrolyzed, carries out ferment afterwards
Female bacterium fermentation, obtains the animal feed for being rich in XOS；
Wherein, the condition of the hydrolysis are as follows: enzymatic hydrolysis pH value is 3-7, hydrolysis temperature is 40-55 DEG C, hydrolysis time 30-
72h；The condition of the fermentation are as follows: 30-40 DEG C of temperature, pH 5.0-6.5, shaking table revolution 200-300rpm/min, fermentation time
24-40h。
According to technical solution above, in preferred situation, hydrolysis described in step 2) and fermentation (cellulose hydrolyzation and ferment
Female bacterium fermentation) progress is synchronized, specifically: zytase is first added into solid obtained in step 1) and is hydrolyzed, adds
Cellulase is hydrolyzed, saccharomycetes to make fermentation, carries out cellulose hydrolyzation simultaneously with saccharomycetes to make fermentation, obtains rich in XOS
Animal feed；
Wherein, the condition of the xylan hydrolysis are as follows: enzymatic hydrolysis pH value is 3-7, hydrolysis temperature is 40-55 DEG C, hydrolysis time
For 8-15h；The condition that the cellulose hydrolyzation and saccharomycetes to make fermentation carry out simultaneously are as follows: 30-40 DEG C of temperature, pH 4.0-
6.0, shaking table revolution 200-300rpm/min, time 30-72h,.
The utility model has the advantages that
1, by the implementation of this technology, pulp and paper making waste is made to get utilization, having reached turns waste into wealth
Purpose reduces the pollution to environment.
2, technical characterstic of the invention is that whole process does not use strong acid and strong base, green high-efficient, high solid-liquid ratio, is not necessarily to outside
Add and energy conservation, water-saving purpose may be implemented under conditions of catalyst, is advantageously implemented the economy of operation.
3, of the invention, by enzyme dosage, enzymatic hydrolysis, fermentation time, the optimization of the conditions such as pH value, hydrolysis item after optimization
Under part, purpose product (XOS) can be efficiently extracted.
4, saccharomycete is added in enzyme hydrolyzate, glucose can be made to be converted into forage protein, realize the increment benefit of glucose
With making XOS ratio or content are opposite to improve, it is contemplated that the full constituent of raw material utilizes, and has good economical, societal benefits.
5, the present invention is not only to make to growth of animal with nutrition and health care the hydrolysis of hemicellulose in waste reed bits
With can replace the XOS product that conventional antibiotic uses, and cellulose is converted into fodder yeast, improve the concentration of XOS
Or content, it is a kind of protein i.e. rich in, amino acid, vitamin, and be rich in the health care type feedstuff of functionality XOS, reality
The higher value application of waste reed bits is showed.
Detailed description of the invention
Fig. 1 is that reed is considered to be worth doing through hydro-thermal prehydrolysis, the preparation of subsequent diastatic fermentation rich in having XOS, Yeast protein animal feed
Flow chart.
Specific embodiment
As hereinafter, the preferred embodiment of the disclosure is described in detail with reference to the accompanying drawings.Before describing, should
Understand the term used in the description and the appended claims, and should not be interpreted as limited to general and dictionary meaning, but
The principle that term should be suitably defined based on the explanation for allowing inventor best, based on corresponding to the technology of the present invention level
Meaning and concept explain.Therefore, the preferred embodiment of description in this only for illustration purpose, and not refer to limit this hair
Bright range, it is therefore to be understood that, can make without departing from the spirit and scope of the invention other it is equivalent implement and
Modification.
The effective XOS raw material of exploiting economy and simple, safe, inexpensive technique be solve XOS it is expensive, be difficult to by
The key utilized extensively.Reed considers the waste material as reed paper mill to be worth doing, containing higher hemicellulose level, less lignin,
The characteristics of with itself without further crushing, being easy to hydrolyzed liquid infiltration, be the desirable feedstock for preparing XOS.
Hydrothermal pretreatment is not necessarily to additional organic and inorganic chemical catalyst, and acetyl group falls off to form acetic acid in hydrolytic process
Weakly acidic condition is formed, in hydrolyzate the impurity phases such as monosaccharide and furfural, phenolic are few to weak acid treatment content, are conducive to the later period and produce
The purifying of object and the processing of waste water and recycling.
The reed bits raw material is preprocessed according to the present invention, fermenting obtains the method rich in having XOS, Yeast protein,
Liquid-solid ratio (volume: quality) is that 10:1-4:1 is optimized for 8:1-4:1 in step 1), and temperature range is optimized for by 140 DEG C -190 DEG C
150 DEG C -170 DEG C, when being lower than 150 DEG C, raw fibre cannot be also seldom by good swollen, delignification, when being higher than 170 DEG C,
Partial reduction sugar is further degraded to furfural substance, causes sugar loss.
The dosage of zytase is optimized for 100-300IU/g, more preferably by 100-600IU/g substrate in step 2)
100-160IU/g；Fiber enzyme dosage is by 10-80FPU/g substrate, preferably 30-50FPU/g substrate；Hydrolyze substrate quality percentage
Specific concentration is by 2%-10%, preferably 6%-10%；Enzymatic hydrolysis (the xylan that cellulose hydrolyzation and saccharomycetes to make fermentation substep carry out
Enzyme and cellulose hydrolyzation) pH value by 3-7 be preferably 4-6, enzymatic hydrolysis (zytase and cellulose hydrolyzation) temperature be 40-55
℃；The time that cellulose hydrolyzation and saccharomycetes to make fermentation carry out simultaneously is optimized for 30-48h by 30-72h, when being less than 30h, fermentation
Reduced sugar residual is more in liquid, and when being greater than 48h, reduced sugar is depleted in fermentation liquid, and XOS may partially assimilate.
Nitrogen source in step 3) with volume mass than 0.5-20% is added, preferably 2-10%, more preferably 5%-
8%, when dosage is less than 5%, Yeast proliferation is slow, and reduced sugar elapsed time is long and utilization is not enough, while toxic metabolite produces
The accumulation of object is also unfavorable for the proliferation of saccharomycete, and when dosage is greater than 8%, metabolism of yeasts is vigorous, and XOS degradation is serious, product matter
Amount decline.Its nitrogen source is by urea or yeast extract, the more optimized urea more cheap for price.
Following embodiment is enumerated only as the example of embodiment of the present invention, does not constitute any limit to the present invention
System, it will be appreciated by those skilled in the art that modification in the range of without departing from essence and design of the invention each falls within the present invention
Protection scope.
Embodiment 1
Reed bits raw material is separated through 50 meshes, removes the dust of small particle.Add after being mixed by liquid-solid ratio 8:1 with a certain amount of water
Enter into the electric cooking pot (Xianyang, the Shaanxi ZQS1-E) of 15L volume, 170 DEG C of heating mixture 30min.Reach regulation
Reaction time after be cooled to room temperature, be separated by solid-liquid separation while hot, with 10 times of volume water washing solid portions, collect and contain XOS and list
The pre-hydrolyzed solution of sugar.
Citric acid-lemon of the obtained solid with the solid content (relative to over dry raw material) of 7% (w/v), with pH4.8 after prehydrolysis
The mixing of lemon acid sodium buffer is added zytase (120IU/g) after 50 DEG C of hydrolysis 10h, is separated by solid-liquid separation, enzyme deactivation, pre-hydrolyzed solution
It is added in the solid after xylanase hydrolysis, is concentrated by evaporation to body in the ratio with xylanase hydrolysis liquid volume ratio 1:1
Cellulase (30FPU/g) is added after long-pending half, additionally incorporates the urea of 6% (w/v), the MgSO of 0.5% (w/v)4,
The KH of 0.5% (w/v)2PO4, adjusting pH is 5.0, is inoculated with the activation candida utili of 5% (v/v) in 30 DEG C, shaking table revolution
Aerobic fermentation 36h under the conditions of 250rpm/min.
XOS, glucose, the composition transfer of xylose and saccharomycete vigor, DCW before analysis inoculation, after fermentation in liquid phase
(absolute dry mass of Yeast protein in unit volume).The results are shown in Table 1.
It can be seen that from the data of 1 embodiment 1 of table and be increased to 73.21% by 39.23% by the ratio for the XOS that ferments, mention
High by 33.98%, glucose content is reduced to 0.67g/L by 10.01g/L, and saccharomycete vigor (viable count of unit volume) reaches
To 3.4 × 1010CFU/mL, Yeast protein content DCW are successfully prepared xylo-oligosaccharide by the implementation of the technique for 5.6g/L
With all very high feed of Yeast protein content, the high level comprehensive utilization of reed is realized.
Embodiment 2
Reed considers the preprocessing process of raw material to be worth doing with embodiment 1.
Citric acid-lemon of the obtained solid with the solid content (relative to over dry raw material) of 7% (w/v), with pH4.8 after prehydrolysis
The mixing of lemon acid sodium buffer is added zytase (120IU/g) in 50 DEG C of hydrolysis 10h, adds cellulase (30FPU/g) and exist
After 50 DEG C of hydrolysis 40h, two step enzymolysis liquid high temperature enzyme deactivations, after pre-hydrolyzed solution with the ratio of two step enzymolysis liquid volume ratio 1:2 in mixing
It is concentrated by evaporation to the 1/3 of total volume, the urea of 6% (w/v), the MgSO of 0.5% (w/v) is added4, 0.5% (w/v) KH2PO4,
Adjusting pH is 5.5, and the activation candida utili for being inoculated with 3% (v/v) is aerobic under the conditions of 30 DEG C, shaking table revolution 250rpm/min
Ferment 36h.
Before analysis inoculation, XOS, glucose, the composition transfer of xylan and saccharomycete vigor, DCW in liquid phase after fermentation.Knot
Fruit is listed in Table 1 below.
The ratio that can be seen that XOS after candida utili ferments from the data of embodiment 2 in table 1 is improved by 20.51%
To 70.12%, glucose content is reduced to 0.91g/L by the 22.21g/L before fermenting, and saccharomycete vigor be (unit volume
Viable count) then reach 1.5 × 1010CFU/mL, Yeast protein content DCW are 4.7g/L.
Embodiment 3
Reed bits raw material is separated through 50 meshes, removes the dust of small particle.Addition is mixed with a certain amount of water by liquid-solid ratio 8:1
Into the electric cooking pot (Xianyang, the Shaanxi ZQS1-E) of 15L volume, 160 DEG C of heating mixture 50min.As defined in reaching
It is cooled to room temperature after reaction time, is separated by solid-liquid separation while hot, with 10 times of volume water washing solid portions, collects and contains XOS and monosaccharide
Pre-hydrolyzed solution.
Citric acid-lemon of the obtained solid with the solid content (relative to over dry raw material) of 6% (w/v), with pH4.8 after prehydrolysis
The mixing of lemon acid sodium buffer, pre-hydrolyzed solution is in the ratio with enzymatic hydrolysis buffer (citric acid-sodium citrate buffer solution) volume ratio 2:5
Be added, be concentrated by evaporation to be added after the half of total volume zytase (140IU/g), cellulase (30FPU/g), at 50 DEG C
After hydrolyzing 40h, the urea of 6% (w/v), the MgSO of 0.5% (w/v) is added4, 0.5% (w/v) KH2PO4, adjusting pH is
5.0, the activation candida utili of 5% (v/v) of inoculation aerobic fermentation 36h under the conditions of 30 DEG C, shaking table revolution 250rpm/min.
XOS, glucose, the composition transfer of xylose and saccharomycete vigor, DCW before analysis inoculation, after fermentation in liquid phase
(absolute dry mass of Yeast protein in unit volume).The results are shown in Table 1.
It can be seen that from the data of 1 embodiment 3 of table and be increased to 68.06% by 29.23% by the ratio for the XOS that ferments, Portugal
Grape sugared content is reduced to 1.52g/L by 24.01g/L, and saccharomycete vigor (viable count of unit volume) reaches 2.9 × 1010CFU/
ML, Yeast protein content DCW are successfully prepared xylo-oligosaccharide and Yeast protein content by the implementation of the technique for 6.1g/L
All very high feed realizes the high level comprehensive utilization of reed bits.
1 reed of table considers the comparison that the ingredient of candida utili fermentation front and back is added in hydrolyzate to be worth doing
Note: saccharomycete vigor (CFU/ml): contained viable count in unit volume,
DCW (g/L): obtaining the absolute dry mass of Yeast protein in unit volume,
XOS ratio (%): XOS content accounts for monosaccharide and the ratio of XOS.
Claims (10)
1. a kind of method for considering production animal feed to be worth doing using reed, which comprises the following steps:
1) reed bits are added to the water after carrying out hydrothermal pretreatment, are separated by solid-liquid separation；
2) zytase is added into solid obtained in step 1) and cellulase is hydrolyzed, saccharomycetes to make fermentation, obtain rich
Animal feed containing XOS.
2. the method according to claim 1, wherein the reed bits are that reed slurry factory slurrying melting process produces
Raw clast.
3. the method according to claim 1, wherein the condition of hydrothermal pretreatment described in step 1) are as follows: liquid
Gu 140-190 DEG C of temperature, handling time 20-60min than 10:1-4:1.
4. the method according to claim 1, wherein zytase dosage is the bottom 100-600IU/g in step 2)
Object, fiber enzyme dosage are 10-50FPU/g substrate, and hydrolysis substrate mass percent concentration is 2%-10%.
5. the method according to claim 1, wherein enzyme hydrolysis process is first xylanase hydrolysis in step 2),
The synchronous hydrolysis process that the fractional hydrolysis process of cellulose hydrolyzation or zytase and cellulase are added simultaneously again.
6. the method according to claim 1, wherein step 2) when carrying out saccharomycetes to make fermentation, is by step 1)
Obtained in hydrolysis obtains in liquid and step 2) liquid mix, using mixed liquid as culture medium, the culture medium
Also need the nitrogen source of additional 0.5-20% (w/v), the KH of 0.5-2% (w/v)2PO4, the MgSO of 0.5-1.5% (w/v)4。
7. according to the method described in claim 6, it is characterized in that, the nitrogen source is urea or yeast extract.
8. the method according to claim 1, wherein the saccharomycete is pichia stipitis in step 2)
(Pichia stipitis), trichosporon cutaneum (Trichosporonsp) or saccharomyces cerevisiae, inoculum concentration are 3-10% (v/v).
9. it is carried out the method according to claim 1, wherein being hydrolyzed in step 2) with fermentation substep, specifically: to
Zytase is first added in solid obtained in step 1) and cellulase is hydrolyzed, then carries out saccharomycetes to make fermentation, obtains rich
Animal feed containing XOS；Or zytase is first added into solid obtained in step 1) and is hydrolyzed, adds cellulose
Enzyme is hydrolyzed, and carries out saccharomycetes to make fermentation afterwards, obtains the animal feed for being rich in XOS；
Wherein, the condition of the hydrolysis are as follows: pH 3-7,40-55 DEG C of temperature, time 30-72h；The fermentation condition are as follows: temperature
30-40 DEG C, pH 5.0-6.5, shaking table revolution 200-300rpm/min, time 24-40h.
10. it is carried out the method according to claim 1, wherein hydrolysis is synchronous with fermentation in step 2), specifically:
Zytase is first added into solid obtained in step 1) to be hydrolyzed, adds cellulase and is hydrolyzed, saccharomycete hair
Ferment carries out cellulose hydrolyzation simultaneously with saccharomycetes to make fermentation, obtains the animal feed for being rich in XOS；
Wherein, the condition of the xylanase hydrolysis are as follows: pH 3-7,40-55 DEG C of temperature, time 8-15h；The cellulase water
The condition that solution is carried out simultaneously with saccharomycetes to make fermentation are as follows: 30-40 DEG C of temperature, pH 4.0-6.0, shaking table revolution 200-300rpm/min,
Time 30-72h.
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