CN109085046A - A kind of preparation method of leaf of bamboo internal anatomy 3 dimensional drawing - Google Patents
A kind of preparation method of leaf of bamboo internal anatomy 3 dimensional drawing Download PDFInfo
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- CN109085046A CN109085046A CN201811256222.4A CN201811256222A CN109085046A CN 109085046 A CN109085046 A CN 109085046A CN 201811256222 A CN201811256222 A CN 201811256222A CN 109085046 A CN109085046 A CN 109085046A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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Abstract
The invention discloses a kind of preparation methods of leaf of bamboo internal anatomy 3 dimensional drawing, after leaf of bamboo acquisition, first immediately with fixed in 70%FAA liquid, rehydration is carried out again, then silicon is carried out using hydrofluoric acid to pre-process, dehydration and the film-making of routine paraffin wax flaking method are carried out again, and Leica DM2500 optical microphotograph sem observation takes pictures, and Photoshop CS6 pieces together leaf of bamboo schematic three dimensional views.The present invention obtains the piece cutting structure in three faces of the leaf of bamboo using paraffin wax flaking, then is spliced with Photoshop CS6 software, easy to operate, can obtain the leaf of bamboo three-dimensional structure figure of complete display.Test confirms, after the paraffin wax flaking for obtaining three, leaf of bamboo slices, the figure of each slice Photoshop CS6 is handled, the leaf of bamboo internal structure stereotome of high quality can be obtained.Therefore the present invention is a kind of technology of effectively observation leaf of bamboo inside stereomicroscopy structure, solves the problems, such as that a certain section can only be observed in bamboo plant leaf of bamboo slicing processes, improves bamboo plant leaf of bamboo structure observation method to establish and enriching and lays the foundation.
Description
Technical field
The invention belongs to bamboo plant displaing microstructure observing technical fields, and in particular to one kind by paraffin section and
Photoshop CS6 software pieces together leaf of bamboo schematic three dimensional views, the method to obtain leaf of bamboo piece complete intra solid anatomical structure.
Background technique
Bambusoideae is a kind of important economic tree, bamboo plant wide variety, warp as a major class in grass family
Ji high efficiency is the object of many scholars' researchs.In the research of early stage, people pass through to bamboo each section nutrition organs, root
[Raechal L J, Curtis J D.Root anatomy of the Bambusoideae (Poaceae) [J]
.American Journal ofBotany, 1990,77 (4): 475-482], subterranean stem [Ding Y L, Grosser D,
Liese W, et al.Anatomic studies on the rhizomes of some monopodial bamboos [J]
.Chinese Journal of Botany, 1993,5 (2): 122-129], vascular bundle [the Chinese Bamboos vascular bundle such as Wen Taihui
Anatomic form research (one of) [J] Bamboo research transactions, 1989.3 (1): 1-21], leaf [Qian Lingyuan, the big 16 Bamboo Species In China in side
The Comparative anatomy of the leaf of bamboo studies [J] bamboo journal, 1986,5 (2): 78-86] anatomic form research, as bamboo system
Classificatory foundation.Among a variety of taxology features, since the anatomical structure of the leaf of bamboo is unique, so that many people are by the sight of research
The anatomic form of the leaf of bamboo is concentrated on to the meaning played on bamboo toxonomy.In Brawn [Brawn W V.Leaf in 1958
Anatomy in grass systematica [J] .Botanical Gazette, 1958,119 (3): 170-178] to 101
After kind gramineae plant is dissected relatively, the leaf portion anatomical structure of gramineae plant is divided into bamboo type (Bambusoid), broomcorn millet type
(Panicoid), giantreed type (Arundoid), Chloris virgata type (Chloridoid), fescue grass type (Festucoid), three-awn type
(Aristidoid) six class.The shuttle occurred in the finger-like arm cell (arm cells) and mesophyll wherein shown on leaf of bamboo cross section
Type cell (fusoid cells) [Metcalfe C R.Some thoughts on the structure of Bamboo
Leaves [J] .Botanical Magazine Tokyo, 2006,69 (820-821): 391-400] also become Bambusoideae plant
The important taxology feature of object.
First and side big [Qian Lingyuan, Comparative anatomy research [J] bamboo of the big 16 Bamboo Species In China leaf of bamboo in side are led from 1986 in China
Son research transactions .1986,5 (2): 78-86] 16 kinds of domestic leaf of bamboves are carried out with the Comparative anatomy of system after, Liu You congruence people [Liu You
Entirely, the form that Liang Dali, He You encourage bamboo class mesophyll cell compares [J] forest-science, 1986,22 (2): 172-177] again to 10
The bamboo class mesophyll cell that kind does not belong to carries out Morphological comparison.[Ding Yulong, Zhao Qiseng, Chen Zhiyin wait bamboo to Ding Yulong in 1994
The Comparative anatomy of impeller structure and its evaluation [J] Nanjing Forestry University journal .1994 to genealogical classification meaning, 18 (3): 1-6]
Et al. the comparative observation of crosscutting longitudinal cutting structure is carried out to the trophophyll of 24 kinds of bamboos after propose, understand the change of leaf microstructure
Change mode has larger value to the genealogical classification of bamboo plant.Nineteen ninety-five Zhao Hui as and the Gong Zhunan [south Zhao Huiru, Gong Zhu bamboo
The internal anatomy and phylogeny [J] Journal of Nanjing Normal University (natural science edition) of class blade, 1995 (4): 102-108] belong to 21
The upper lower epidermis of 27 kinds of bamboo kind leaf of bamboves and crosscutting observation carry out the comparison pusher of system to mesophyll cell and vascular bundle Fusoid cells
Measure evolution status of each bamboo kind in genealogical classification.
Forefathers to the anatomic observation of the leaf of bamboo are divided up from the crosscutting mesophyll cell form of epidermal structure and the leaf of bamboo of the leaf of bamboo
Existing difference between more each bamboo kind leaf of bamboo is analysed, and so far not from three sections of blade, comprehensively observe the entirety of the leaf of bamboo
Three-dimensional structural feature.
Summary of the invention
Goal of the invention: the deficiencies in the prior art are directed to, the object of the present invention is to provide a kind of bamboo plant blades
The method of stereomicroscopy structure observation overcomes and observes the problems such as leaf of bamboo structure is only limitted to single section.
Technical solution: in order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention are as follows:
A kind of preparation method of leaf of bamboo internal anatomy 3 dimensional drawing, after leaf of bamboo acquisition, first immediately in 70%FAA liquid
Middle fixation；Rehydration is carried out again；Then silicon is carried out using hydrofluoric acid to pre-process, then carry out dehydration and routine paraffin wax flaking legal system
Piece obtains the longitudal section and cross-sectional slice of the leaf of bamboo；The fast green dual staining dyeing of sarranine, neutral gum mounting obtain the leaf of bamboo
Longitudal section and Structure of cross section figure；Leaf of bamboo sample after FAA is fixed, by alcohol serial dehydration, dimethylbenzene is transparent overnight, changes
Direct microscopy obtains side grain structure chart after to 70% alcohol；Use picture mosaic software by longitudal section, cross section and side grain structure again
Figure pieces together out leaf of bamboo schematic three dimensional views.
The preparation method of the leaf of bamboo internal anatomy 3 dimensional drawing, the healthy functions that random acquisition is unfolded completely
The leaf of bamboo, and cut rapidly in the middle part of the leaf of bamboo with sharp cutter, size is 0.5cm × 0.5cm.
The preparation method of the leaf of bamboo internal anatomy 3 dimensional drawing, is immediately placed in fixer for the leaf of bamboo of acquisition
Fixed at least 48h in 70%FAA takes out the leaf of bamboo after fixing and carries out the processing of gradient rehydration.
Rehydration processing: the preparation method of the leaf of bamboo internal anatomy 3 dimensional drawing is respectively put blade taking-up
Set -30% -15% alcohol of alcohol of 50% alcohol-each 1h of pure water.
The preparation method of the leaf of bamboo internal anatomy 3 dimensional drawing goes silicon pretreatment as the leaf of bamboo is placed in 25%
36-48h is handled in hydrofluoric acid.
The preparation method of the leaf of bamboo internal anatomy 3 dimensional drawing, goes silicon after treatment, the leaf of bamboo is taken out,
After being cleaned with pure water, gradient alcohol dehydration is carried out, the leaf of bamboo is successively placed in -70% wine of -50% alcohol of -30% alcohol of 15% alcohol
Each 1h of essence.
The preparation method of the leaf of bamboo internal anatomy 3 dimensional drawing, routine paraffin wax flaking method are as follows: routine paraffin wax
Method of tableting are as follows: the good leaf of bamboo of pre-processing is dehydrated step by step through 70%, 80%, 90% ethyl alcohol, every grade of stop 2h, after again with anhydrous
Ethanol dehydration 2 times, 1h is stopped every time；It is anhydrous that the leaf of bamboo after dehydration is transferred to 1: 1,1: 3 dimethylbenzene-respectively from dehydrated alcohol
In alcohol mixeding liquid, stop 1.5h at different levels, after be transferred in pure dimethylbenzene 2 times, stop 1h every time, carry out transparent processing；By paraffin
Be made into mixed liquor in 1: 2 ratio with dimethylbenzene, transparent good material be put into above-mentioned mixed liquor in 38 DEG C of oven overnights, after more
It changes 1: 1 paraffin-dimethylbenzene mixed liquor to be transferred to again in pure wax 2 times in 58 DEG C of baking oven 2h, stops 1h every time；Finally embedded；So
(Leica RM2255) automatic clinical microtome is used to cut the slice with a thickness of 8 μm afterwards.
The preparation method of the leaf of bamboo internal anatomy 3 dimensional drawing, after slice is carried out, Leica DM2500 light
Learn microscopically observation.
The preparation method of the leaf of bamboo internal anatomy 3 dimensional drawing after three face structure of the leaf of bamboo obtains, is used
Photoshop CS6 software pieces together leaf of bamboo schematic three dimensional views.
The utility model has the advantages that compared with prior art, the present invention can get the longitudal section of the leaf of bamboo of high quality using paraffin wax flaking
(being parallel to lateral vein), cross section (perpendicular to lateral vein) picture and the truncation structure for being parallel to blade, and with Photoshop CS6
Software pieces together leaf of bamboo schematic three dimensional views, easy to operate, can obtain complete leaf of bamboo stereomicroscopy structure.Therefore the present invention is one
The kind effectively observation microstructural technology of leaf of bamboo interior three-dimensional, efficiently solves the unilateral observation of bamboo plant leaf of bamboo internal structure
Problem provides method to observe and recognizing leaf of bamboo internal structure visual in imagely, has good practicability.
Detailed description of the invention
Fig. 1 is seven color red bamboo schematic three dimensional views and each section optical microscopy map；In figure, the color red bamboo three-dimensional splicing signal of a. seven
Figure；B. leaf of bamboo sectional view, section is perpendicular to lateral vein；C. the longitudinal sectional figure of the leaf of bamboo, section are parallel to lateral vein；D. leaf of bamboo truncation, section are flat
Row is in leaf epidermis；
Fig. 2 is beautiful bamboo schematic three dimensional views and each section optical microscopy map；In figure, the beautiful bamboo three-dimensional splicing schematic diagram of a.；B. bamboo
Leaf sectional view, section is perpendicular to lateral vein；C. the longitudinal sectional figure of the leaf of bamboo, section are parallel to lateral vein；D. leaf of bamboo truncation, section are parallel to leaf table
Skin；
Fig. 3 is Sasa fortunei schematic three dimensional views and each section optical microscopy map；In figure, a. Sasa fortunei three-dimensional splicing schematic diagram；
B. leaf of bamboo sectional view, section is perpendicular to lateral vein；C. the longitudinal sectional figure of the leaf of bamboo, section are parallel to lateral vein；D. leaf of bamboo truncation, section are parallel to
Leaf epidermis；Fig. 4 is that the hydrofluoric acid of henon bamboo other concentration removes silicon effect picture, and equal display effect is poor.
Fig. 4 is bright and beautiful bamboo schematic three dimensional views and each section optical microscopy map；In figure, a. brocade bamboo three-dimensional splicing schematic diagram；B. bamboo
Leaf sectional view, section is perpendicular to lateral vein；C. the longitudinal sectional figure of the leaf of bamboo, section are parallel to lateral vein；D. leaf of bamboo truncation, section are parallel to leaf table
Skin；
Fig. 5 is yellow Buddha's warrior attendant bamboo schematic three dimensional views and each section optical microscopy map；In figure, a. Huang Buddha's warrior attendant bamboo three-dimensional splicing
Schematic diagram；B. leaf of bamboo sectional view, section is perpendicular to lateral vein；C. the longitudinal sectional figure of the leaf of bamboo, section are parallel to lateral vein；D. leaf of bamboo truncation is cut
Face is parallel to leaf epidermis；
Fig. 6 is floral leaf Tang Zhu schematic three dimensional views and each section optical microscopy map；In figure, the signal of a. floral leaf Tang Zhu three-dimensional splicing
Figure；B. leaf of bamboo sectional view, section is perpendicular to lateral vein；C. the longitudinal sectional figure of the leaf of bamboo, section are parallel to lateral vein；D. leaf of bamboo truncation, section are flat
Row is in leaf epidermis.
Specific embodiment
The present invention is described further combined with specific embodiments below.
Embodiment 1
A kind of preparation method of leaf of bamboo internal anatomy 3 dimensional drawing, comprising the following steps:
1) test material is six kinds of bamboo plant leaf of bamboves, is acquired in mid-January, 2018 in Nanjing, details such as table 1
The healthy functions leaf of bamboo shown, that random acquisition is unfolded completely, and cut rapidly in the middle part of the leaf of bamboo with sharp cutter, slice size is
0.5cm × 0.5cm, immediately with fixed in 70%FAA liquid after adopting.
16 bamboo kinds of table acquire information table
2) after fixed 48h, carry out rehydration: it is pure that blade is taken out placement -15% alcohol of -30% alcohol of 50% alcohol-respectively
Each 1h of water；Handle after rehydration carrying out silicon, by after each bamboo kind blade rehydration with 25% hydrofluoric acid treatment 36-48h, then to bamboo
Leaf carries out gradient alcohol dehydration (- 70% each 1h of alcohol of -50% alcohol of -30% alcohol of 15% alcohol) according still further to routine paraffin wax flaking
Then method film-making carries out the film-making of routine paraffin wax flaking method that is, using going silicon to pre-process.
Routine paraffin wax flaking method are as follows: be dehydrated the leaf of bamboo fixed step by step through 70%, 80%, 90% ethyl alcohol, every grade of stop
2h, after again with dehydrated alcohol be dehydrated 2 times, stop 1h every time；The leaf of bamboo after dehydration is transferred to 1: 1,1 respectively from dehydrated alcohol
: in 3 dimethylbenzene-dehydrated alcohol mixed liquor, stop 1.5h at different levels, after be transferred in pure dimethylbenzene 2 times, stop 1h every time, carry out saturating
Daylight reason；Paraffin and dimethylbenzene are made into mixed liquor in 1: 2 ratio, transparent good material is put into above-mentioned mixed liquor in 38 DEG C
Oven overnight is replaced 1: 1 paraffin-dimethylbenzene mixed liquor afterwards and is transferred in pure wax again 2 times in 58 DEG C of baking oven 2h, stops 1h every time；Most
After embedded；Then it uses (Leica RM2255) automatic clinical microtome to cut the slice with a thickness of 8 μm, obtains the longitudinal sectional of the leaf of bamboo
Face and cross-sectional slice；The fast green dual staining dyeing of sarranine, neutral gum mounting.After slice is carried out, Leica DM2500 light
Microscopically observation is learned to take pictures.
3) be parallel to the truncation structure film-making of blade: the leaf of bamboo sample after FAA is fixed passes through alcohol serial dehydration, diformazan
Benzene is transparent overnight, shifts to direct microscopy after 70% alcohol.
4) after three face structure of the leaf of bamboo obtains, " editor-is carried out to three plane pictures with picture mosaic softwares of Photoshop CS6
Transformation-beveling " adjustment, then picture adjusted is pieced together into leaf of bamboo schematic three dimensional views.
(Fig. 1-Fig. 6) is as can be seen that six bamboo kind blade interior structural arrangements are whole from the schematic three dimensional views of six bamboo kinds
It is neat regular, it is obvious that close layering is arranged between mesophyll cell.Green parenchymal tissue in mesophyll includes finger-like arm cell and plum
Flower-shaped cell, each bamboo kind is finger-like arm cell close to one layer of mesophyll cell of blade epicuticle, on cross section, cell wall
Downward infolding, neat finger-like are arranged on the inside of epicuticle.From on longitudal section, cell is in long strip, lower part cell wall infolding
It is less, and strip is shown as in side grain from top to bottom to scattered round shape, infer that its cell three-dimensional structure should be two layers of palm weight
It is stacked, the finger-like arm cellular morphology no significant difference of each bamboo kind；Remaining mesophyll cell is plum blossom-shaped.From longitudal section it can be seen that
The seven color red leaf of bamboo meat cell numbers of plies are minimum, are 4 layers；The mesophyll cell number of plies of beautiful bamboo is most, is 6 layers；Remaining Sasa fortunei, yellow item gold
Rigid bamboo, the floral leaf Tang Zhu mesophyll cell spore number of plies are of five storeys in arrangement most many places.Bubble is distributed in epicuticle between two lateral vein of blade
It is thin by long cell, short cell and blister to can be seen that each bamboo kind epicuticle from the epicuticle micro-image in each figure for shape cell
Born of the same parents' composition, from the visible bulliform cell in cross section compared with length cell, close to skin portion cell wall without thickening cuticula.Six
Have Fusoid cells in a bamboo kind blade, by the schematic three dimensional views and three face slice maps of this six bamboo kinds it will be seen that
The small bending broad in the middle in both ends is presented in Fusoid cells on cross section, is shrinkage flat " I " in longitudinal sectional and side grain
Type.Fusoid cells quantity is most in seven color red bamboos, lineae ablicantes yin-yang bamboo and floral leaf Tang Zhu, in blade Fusoid cells in bulliform cell and
Position between lateral vein longitudinally forms a line, and together with bulliform cell column and lateral vein, runs through entire blade.The shuttle-type of Sasa fortunei is thin
It can be seen that, in the plum blossom-shaped mesophyll cell of the 4th row, accidentally there are some Fusoid cells rows from longitudal section in born of the same parents' negligible amounts
Column are wherein.And Fusoid cells are then seldom seen in the mesophyll cell of yellow Buddha's warrior attendant bamboo and beautiful bamboo.Six blades in the present embodiment
Also small crossvein is all occurred, from the translucent blade film-making of each bamboo kind, it can be found that transverse vein is connected with the lateral vein of the right and left
It connects, the mechanical tissue that blade side grain and longitudal section can see transverse vein and its be connected with upper lower epidermis.It is constructed by three-dimensional figure,
D structure inside the bamboo plant leaf of bamboo can be well understood.
Claims (9)
1. a kind of preparation method of leaf of bamboo internal anatomy 3 dimensional drawing, which is characterized in that after leaf of bamboo acquisition, first exist immediately
It is fixed in 70%FAA liquid；Rehydration is carried out again；Then silicon is carried out using hydrofluoric acid to pre-process, then carry out dehydration and routine paraffin wax
Method of tableting film-making obtains the longitudal section and cross-sectional slice of the leaf of bamboo；The fast green dual staining dyeing of sarranine, neutral gum mounting,
Observation obtains the longitudal section and Structure of cross section figure of the leaf of bamboo；Leaf of bamboo sample after FAA is fixed, passes through alcohol serial dehydration, diformazan
Benzene is transparent overnight, shifts to directly microscopy after 70% alcohol and obtains side grain structure chart；Use picture mosaic software by longitudal section, cross section again
It pieces together out leaf of bamboo schematic three dimensional views with side grain structure chart.
2. the preparation method of leaf of bamboo internal anatomy 3 dimensional drawing according to claim 1, which is characterized in that random
The healthy functions leaf of bamboo unfolded completely is acquired, and is cut rapidly in the middle part of the leaf of bamboo with sharp cutter, size is 0.5cm × 0.5cm.
3. the preparation method of leaf of bamboo internal anatomy 3 dimensional drawing according to claim 1, which is characterized in that will adopt
The leaf of bamboo of collection is immediately placed in fixer 70%FAA fixed at least 48h, takes out the leaf of bamboo after fixing and carries out the processing of gradient rehydration.
4. the preparation method of leaf of bamboo internal anatomy 3 dimensional drawing according to claim 1 or 3, which is characterized in that
Rehydration processing: blade is taken out place -30% -15% alcohol of alcohol of 50% alcohol-each 1h of pure water respectively.
5. the preparation method of leaf of bamboo internal anatomy 3 dimensional drawing according to claim 1, which is characterized in that remove silicon
Pretreatment handles 36-48h for the leaf of bamboo to be placed in 25% hydrofluoric acid.
6. the preparation method of leaf of bamboo internal anatomy 3 dimensional drawing according to claim 1, which is characterized in that remove silicon
After treatment takes out the leaf of bamboo, after being cleaned with pure water, carries out gradient alcohol dehydration: the leaf of bamboo being successively placed in 15% alcohol-
- 70% each 1h of alcohol of -50% alcohol of 30% alcohol.
7. the preparation method of leaf of bamboo internal anatomy 3 dimensional drawing according to claim 1, which is characterized in that conventional
Paraffin method are as follows: the leaf of bamboo fixed is dehydrated step by step through 70%, 80%, 90% ethyl alcohol, every grade of stop 2h, after again with anhydrous
Ethanol dehydration 2 times, 1h is stopped every time；It is anhydrous that the leaf of bamboo after dehydration is transferred to 1: 1,1: 3 dimethylbenzene-respectively from dehydrated alcohol
In alcohol mixeding liquid, stop 1.5h at different levels, after be transferred in pure dimethylbenzene 2 times, stop 1h every time, carry out transparent processing；By paraffin
Be made into mixed liquor in 1: 2 ratio with dimethylbenzene, transparent good material be put into above-mentioned mixed liquor in 38 DEG C of oven overnights, after more
It changes 1: 1 paraffin-dimethylbenzene mixed liquor to be transferred to again in pure wax 2 times in 58 DEG C of baking oven 2h, stops 1h every time；Finally embedded；So
The slice with a thickness of 8 μm is cut using automatic clinical microtome afterwards.
8. the preparation method of leaf of bamboo internal anatomy 3 dimensional drawing according to claim 1, which is characterized in that slice
After carrying out, Leica DM2500 optical microphotograph is under the microscope.
9. the preparation method of leaf of bamboo internal anatomy 3 dimensional drawing according to claim 1, which is characterized in that the leaf of bamboo
After three face structures obtain, pieced together leaf of bamboo schematic three dimensional views with Photoshop CS6 software.
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Citations (2)
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| CN101915693A (en) * | 2010-07-07 | 2010-12-15 | 新疆医科大学 | Human embryonic trigeminus based three-dimensional reconstruction method by using histotomy staining |
| CN102721583A (en) * | 2012-06-01 | 2012-10-10 | 河南科技大学 | Method for producing film of leaf epidermis of stomatal pit of sweetscented oleander leaf |
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2018
- 2018-10-25 CN CN201811256222.4A patent/CN109085046B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101915693A (en) * | 2010-07-07 | 2010-12-15 | 新疆医科大学 | Human embryonic trigeminus based three-dimensional reconstruction method by using histotomy staining |
| CN102721583A (en) * | 2012-06-01 | 2012-10-10 | 河南科技大学 | Method for producing film of leaf epidermis of stomatal pit of sweetscented oleander leaf |
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| ELIZABETH FARIS CROWELL等: "Differential Regulation of Cellulose Orientation at the Inner and Outer Face of Epidermal Cells in the Arabidopsis Hypocotyl", 《AMERICAN SOCIETY OF PLANT BIOLOGISTS》 * |
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