CN109061181A - A kind of vascular endothelial growth factor chemiluminescence detection kit - Google Patents
A kind of vascular endothelial growth factor chemiluminescence detection kit Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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Abstract
The invention discloses a kind of vascular endothelial growth factor chemiluminescence detection kits, including reagent and hold the kit body of reagent, reagent includes enzyme labelled antibody, chemiluminescent substrate, concentrated cleaning solution, dilution, standard solution, kit body includes box lower body, lid, crash roll is equipped under box in vivo, fixing seat, the blind hole for placing reagent bottle is equipped in fixing seat, this kit body is more rectangular, and kit is more beautiful, overall structure is more stable, it is hardly damaged when transport, it can be improved the whole safety in utilization of detection kit, save space；Coated VEGF monoclonal antibody and sample to be tested form the sandwich complex structure of " solid-phase coating antibody-determined antigen-enzyme labelled antibody " in the VEGF monoclonal antibody and microwell plate of enzyme label of the present invention, the blank for having filled up vascular endothelial growth factor chemical luminescence reagent kit in the market has the advantages that testing result high sensitivity.
Description
Technical field
The present invention relates to medical immunology external diagnosis reagent fields, and in particular to a kind of vascular endothelial growth factor
Learn luminescence detection kit.
Background technique
Vascular endothelial growth factor (VEGF) is the basic protein for the high glycosylation that a molecular weight is 45KD, is
A kind of promotion vascular endothelial cell division having high degree of specificity, the factor of induction of vascular new life, it can specifically be acted on
Vascular endothelial cell promotes angiogenesis, maintains the normal condition and integrality of blood vessel, improves vasopermeability.Blood vessel endothelium
Porcine HGF participates in many pathology and physiological expression, played in the expression overall process for participating in union it is important because
Element can express enhancing in the pathogenic process of diabetic nephropathy, in addition can in the expression to leukaemia and critical limb ischemia
A kind of drug as gene therapy.The study found that VEGF has expression, such as wound repair in many pathophysiological processes
In vascular endothelial cell and macrophage, diabetic retinopathy cell, cardiovascular and cerebrovascular diseases and liver diseases etc., and with from
Secretion, paracrine and emiocytosis mode specifically the vegf receptor on vasoactive endothelial cell membrane to cause cell
Interior signal transduction and realize.In numerous angiogenesis factors, VEGF is the object that generally acknowledged main induction of vascular generates
Matter, it can strongly promote blood vessel endothelium mitosis and new vessels are formed.
The detection method of prior art vascular endothelial growth factor mainly has enzyme-linked immunization, time-resolved fluorescence to exempt from
Epidemic disease analytic approach, chemoluminescence method etc., however these methods have the range of linearity narrow, the disadvantages of detection time is long, are unable to satisfy and face
Bed demand.
Summary of the invention
The purpose of the present invention is to provide a kind of vascular endothelial growth factor chemiluminescence detection kits, fill up
The blank of vascular endothelial growth factor chemical luminescence reagent kit in the market has testing result high sensitivity, detection range
The advantages that wide.
To achieve the above object, the invention provides the following technical scheme:
A kind of vascular endothelial growth factor chemiluminescence detection kit including reagent and holds the reagent of reagent
Box box body, the reagent include enzyme labelled antibody, chemiluminescent substrate, concentrated cleaning solution, dilution, standard solution, the examination
The section of agent box box body is cylindrical structure, and the kit body includes box lower body, lid, and box lower body is the chamber of open upper end
Room, lid are connected through a screw thread with box lower body, and the diameter of box lower body is d2, are equipped with crash roll, fixing seat, crash roll in vivo under box
For the cylindrical structure of open upper end, fixed seating is higher than the upper end of crash roll, fixing seat in the upper end of fixing seat in crash roll
Interior to be equipped with the first blind hole, the second blind hole, the third blind hole for placing reagent bottle, the first blind hole is located at the middle position of fixing seat, the
Two blind holes, third blind hole are located at the two sides of the first blind hole, hold concentrated cleaning solution, dilution in the first blind hole respectively, the
Enzyme labelled antibody, chemiluminescent substrate are held in two blind holes respectively, standard solution, the lid lower surface are held in third blind hole
It is stained with microwell plate box, is microwell plate in microwell plate box, lid upper surface is equipped with the first groove, and it is recessed to be equipped with second in the first groove
The section of slot, the first groove is circular configuration, and the diameter of the first groove is that the section of d1, d1=d2, the second groove is rectangle
Structure, interior be equipped with of the second groove can 90 ° of folding handles rotated.
A kind of above-mentioned vascular endothelial growth factor chemiluminescence detection kit, wherein the upper end of the box lower body
Outside is equipped with external screw thread, and the inner surface lower end of lid is equipped with internal screw thread, and internal screw thread matches with external screw thread.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the kit body is
Polypropylene material.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the crash roll is sponge
Material.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the fixing seat is poly- third
Alkene material.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the microwell plate is poly- third
Ethylene material.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the internal diameter of the lid is
D3, d3=d2=d1.
A kind of above-mentioned vascular endothelial growth factor chemiluminescence detection kit, wherein the microwell plate coating
VEGF monoclonal antibody, the enzyme labelled antibody are the VEGF monoclonal antibody of horseradish peroxidase-labeled, chemiluminescent substrate
Liquid A is containing 4ug/mL to the Tris-HCl buffer (0.1M, pH=8.5) of iodophenol, 6.5mg/mL luminol, chemiluminescence
Substrate solution B is citrate buffer solution (0.1M, pH=containing 80mg/mL hydrogen peroxide, 20mg/mL horseradish peroxidase
4.5)。
A kind of above-mentioned vascular endothelial growth factor chemiluminescence detection kit, wherein the dilution be by
Thimerosal that 150ml bovine serum albumin(BSA), 25ml weight percent concentration are 1%, 10ml concentration be the 0.1M potassium ferricyanide,
The Tween-20 of 0.5ml, the Proclin-300 that 15ml concentration is the gentamicin of 10mg/ml, 80ml, surplus is PBS buffer solution
(0.01M, pH=7.5) is settled to the solution that 1000ml is mixed to get.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the concentrated cleaning solution is
The NaH for being 2.9g by weight2PO4·2H2O, weight is the Na of 29g2HPO4·12H2O, weight is NaCl, 80ml of 250g
Proclin-300 and 20ml Tween-20 adds distilled water to be settled to the solution that 1000ml is mixed to get.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the standard solution is 6
The VEGF antigen of a concentration gradient, the concentration is successively are as follows: 0pg/ml, 30pg/ml, 125pg/ml, 500pg/ml, 1000pg/
ml、2000pg/ml。
This kit using when also need to use coating buffer, confining liquid, the coating buffer is carbonate buffer solution
(0.03mol/L, pH=9.6), the confining liquid are to be by 0.02g sodium azide, 15g bovine serum albumin(BSA) and 120mL concentration
The solution that 0.03mol/L phosphate solution (pH=7.5) is mixed to get.
The present invention have the utility model has the advantages that
(1) kit body of the present invention is cylindrical structure, and more rectangular kit is more beautiful, and overall structure is more steady
It is fixed, and shape conveniently, reduce preparation cost, when transport is hardly damaged, and is equipped with the crash roll of sponge material under box in vivo, fixed
Seat is located in crash roll, improves the whole safety in utilization of detection kit, reagent will not damage easily when by ambient pressure
Bad, lid is connected through a screw thread with box lower body, is connected firmly, lid upper surface be equipped with the first groove, the diameter of the first groove with
Box lower body is identical, stacks convenient for kit, saves space, is equipped in the second groove and folds handle, and what when use can be convenient takes
Kit is taken, handle is folded when not in use and is located in the second groove, will not impact to kit is stacked, be put in fixing seat
Reagent bottle is set, it is easy to assembly, detection operation is carried out convenient for user；
(2) the VEGF monoclonal antibody of horseradish peroxidase-labeled of the present invention and coated VEGF monoclonal on microwell plate
Antibody and sample to be tested form the sandwich complex structure of " solid-phase coating antibody-determined antigen-enzyme labelled antibody ", and the present invention uses
Double antibody sandwich method and enzymatic chemiluminescence immune assay combine, and efficiently utilize chemiluminescence principle, application
Horseradish peroxidase enzyme catalytic luminous substrate luminol, the optical signal that detection luminol generates, sensitivity greatly improve, this reagent
Each solution formula employed in box body system is the optimal case under the reaction system, this kit range of linearity is wide, linear to close
System is good, and in accuracy height batch and difference between batch meets examination criteria, and order of accuarcy is better than other types of currently on the market
VEGF kit, detection efficiency are high.
Detailed description of the invention
Fig. 1 is kit body structural schematic diagram of the present invention；
Fig. 2 is box lower body schematic diagram；
Fig. 3 is fixing seat, crash roll schematic diagram；
Fig. 4 is fixing seat schematic diagram；
Fig. 5 is A-A diagrammatic cross-section in Fig. 1；
In figure: 1- kit body, 2- box lower body, 3- lid, 4- crash roll, 5- fixing seat, the first blind hole of 6-, 7- second
Blind hole, 8- third blind hole, 9- microwell plate box, the first groove of 10-, the second groove of 11-, 12- fold handle, 13- reagent.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description.
Experimental method used in embodiment is conventional method unless otherwise specified；Material used in embodiment,
Reagent etc., is commercially available unless otherwise specified.
Embodiment 1, the structure of chemical luminescence reagent kit
As shown in Figs. 1-5, a kind of vascular endothelial growth factor chemiluminescence detection kit, including reagent and Sheng
The kit body of reagent is put, the reagent includes enzyme labelled antibody, chemiluminescent substrate, concentrated cleaning solution, dilution, standard items
The section of solution, the kit body is cylindrical structure, and the kit body includes box lower body, lid, and box lower body is
The chamber of open upper end, lid are connected through a screw thread with box lower body, and the diameter of box lower body is d2, be equipped in vivo under box crash roll,
Fixing seat, crash roll are the cylindrical structure of open upper end, and fixed seating is higher than crash roll in the upper end of fixing seat in crash roll
Upper end, the first blind hole, the second blind hole, the third blind hole for placing reagent bottle are equipped in fixing seat, the first blind hole is located at fixing seat
Middle position, the second blind hole, third blind hole are located at the two sides of the first blind hole, hold thickening and washing respectively in the first blind hole
Liquid, dilution hold enzyme labelled antibody, chemiluminescent substrate in the second blind hole respectively, standard solution, institute are held in third blind hole
It states lid lower surface and is stained with microwell plate box, be microwell plate in microwell plate box, lid upper surface is equipped with the first groove, in the first groove
Equipped with the second groove, the section of the first groove is circular configuration, and the diameter of the first groove is cutting for d1, d1=d2, the second groove
Face is rectangle structure, and being equipped in the second groove can 90 ° of folding handle rotated.
A kind of above-mentioned vascular endothelial growth factor chemiluminescence detection kit, wherein the upper end of the box lower body
Outside is equipped with external screw thread, and the inner surface lower end of lid is equipped with internal screw thread, and internal screw thread matches with external screw thread.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the kit body is
Polypropylene material.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the crash roll is sponge
Material.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the fixing seat is poly- third
Alkene material.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the microwell plate is poly- third
Ethylene material.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the internal diameter of the lid is
D3, d3=d2=d1.
Kit body of the present invention is cylindrical structure, and more rectangular kit is more beautiful, and overall structure is more stable, and
And forming is convenient, reduces preparation cost, when transport, is hardly damaged, and is equipped with the crash roll of sponge material, fixed seating under box in vivo
In the whole safety in utilization in crash roll, improving detection kit, reagent will not damage easily when by ambient pressure, lid
Body is connected through a screw thread with box lower body, is connected firmly, and lid upper surface is equipped with the first groove, the diameter and box lower body of the first groove
It is identical, it is stacked convenient for kit, saves space, be equipped in the second groove and fold handle, the reagent of taking that when use can be convenient
Box folds handle when not in use and is located in the second groove, will not impact to kit is stacked, reagent is placed in fixing seat
Bottle, it is easy to assembly, detection operation is carried out convenient for user.
The composition of embodiment 2, chemical luminescence reagent kit
This kit includes stating concentrated cleaning solution, dilution, enzyme labelled antibody, Chemoluminescent substrate A, chemiluminescent substrate
Liquid B, standard solution, coating buffer, confining liquid.
The microwell plate is coated with VEGF monoclonal antibody, and the enzyme labelled antibody is that the VEGF of horseradish peroxidase-labeled is mono-
Clonal antibody, Chemoluminescent substrate A are containing 4ug/mL to the Tris-HCl buffer of iodophenol, 6.5mg/mL luminol
(0.1M, pH=8.5), Chemoluminescent substrate B are the lemon containing 80mg/mL hydrogen peroxide, 20mg/mL horseradish peroxidase
Lemon acid buffer (0.1M, pH=4.5).
A kind of above-mentioned vascular endothelial growth factor chemiluminescence detection kit, wherein the dilution be by
Thimerosal that 150ml bovine serum albumin(BSA), 25ml weight percent concentration are 1%, 10ml concentration be the 0.1M potassium ferricyanide,
The Tween-20 of 0.5ml, the Proclin-300 that 15ml concentration is the gentamicin of 10mg/ml, 80ml, surplus is PBS buffer solution
(0.01M, pH=7.5) is settled to the solution that 1000ml is mixed to get.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the concentrated cleaning solution is
The NaH for being 2.9g by weight2PO4·2H2O, weight is the Na of 29g2HPO4·12H2O, weight is NaCl, 80ml of 250g
Proclin-300 and 20ml Tween-20 adds distilled water to be settled to the solution that 1000ml is mixed to get.
Above-mentioned a kind of vascular endothelial growth factor chemiluminescence detection kit, wherein the standard solution is 6
The VEGF antigen of a concentration gradient, the concentration is successively are as follows: 0pg/ml, 30pg/ml, 125pg/ml, 500pg/ml, 1000pg/
ml、2000pg/ml。
This kit using when also need to use coating buffer, confining liquid, the coating buffer is carbonate buffer solution
(0.03mol/L, pH=9.6), the confining liquid are to be by 0.02g sodium azide, 15g bovine serum albumin(BSA) and 120mL concentration
The solution that 0.03mol/L phosphate solution (pH=7.5) is mixed to get.
Embodiment 3, the preparation method of kit
Standard items are prepared with VEGF positive serum, microwell plate is coated with VEGF monoclonal antibody, horseradish peroxidase-labeled
VEGF monoclonal antibody prepares Chemoluminescent substrate A and B, prepares concentrated cleaning solution and dilution, dispenses above-mentioned component, group
Dress is finished product.
(1) it is coated with: it is mono- to be configured to the VEGF that peridium concentration is 2ug/ml with the carbonate buffer solution that 30mM pH value is 9.6
Clonal antibody be coated with buffer, and by coating buffer be carried in microwell plate (hole 100ul/), 2-8 DEG C coating 18-24 hours.
(2) it closes: being that 0.03mol/L phosphate is molten with 0.02g sodium azide, 15g bovine serum albumin(BSA) and 120mL concentration
The confining liquid that liquid (pH=7.5) is mixed to get closes the microwell plate after above-mentioned washing.
(3) enzyme labelled antibody: taking HRP 5mg to be dissolved in 0.2mol/L, in pH=5.6 acetate buffer 1ml, is added 1%
DNFB ethanol solution 0.1ml, gentle agitation 1h, is added the 0.1mol/LNaIO of fresh configuration at room temperature40.5ml, at this time
Solution becomes blackish green from former brown, and 4 DEG C of placement 30min, solution becomes blackish green from former brown at this time, and 2.5% second two is added
Alcohol 1ml, gentle agitation 1h, terminates reaction, antibody 5-10mg to be marked is added, with 1mol/L, pH=9.5 carbonate at room temperature
Buffer adjusts pH value to 9.0, mixes, set 4 DEG C overnight；Sodium borohydride solution 0.1ml is added, mixes, 4 DEG C of placement 3h are right
0.01mol/L, pH=7.4 phosphate buffer, 4 DEG C of dialysed overnights are changed liquid 3 times, and 3000r/min is centrifuged 30min, removal precipitating
Object, collecting supernatant is enzyme labelled antibody.
(4) chemiluminescent substrate: Chemoluminescent substrate A is containing 4ug/mL to iodophenol, 6.5mg/mL luminol
Tris-HCl buffer (0.1M, pH=8.5), Chemoluminescent substrate B are to contain 80mg/mL hydrogen peroxide, 20mg/mL horseradish
The citrate buffer solution (0.1M, pH=4.5) of catalase.
(5) dilution: 150ml bovine serum albumin(BSA), the thimerosal that 25ml weight percent concentration is 1%, 10ml is dense
Degree is the Proclin- of the 0.1M potassium ferricyanide, the Tween-20 of 0.5ml, the gentamicin that 15ml concentration is 10mg/ml, 80ml
300, surplus is that PBS buffer solution (0.01M, pH=7.5) is settled to the dilution that 1000ml is mixed to get.
(6) concentrated cleaning solution: the NaH for being 2.9g by weight2PO4·2H2O, weight is the Na of 29g2HPO4·12H2O, again
Amount be 250g NaCl, 80ml Proclin-300 and 20ml Tween-20, add distilled water be settled to 1000ml be mixed to get it is dense
Contracting cleaning solution.
(7) it is coated with buffer: for carbonate buffer solution (0.03mol/L, pH=9.6).
(8) confining liquid: being 0.03mol/L phosphate by 0.02g sodium azide, 15g bovine serum albumin(BSA) and 120mL concentration
Solution (pH=7.5) is mixed to get confining liquid.
Embodiment 4, the application method of this kit
(1) microwell plate after taking out coating in 2-8 DEG C of refrigerator, is placed at room temperature for 15 minutes.
(2) each 2 hole of standard items every hole 50ul, sample to be tested 50ul are set, is incubated 30 minutes for 37 DEG C after oscillation mixes.
(3) dereaction liquid is got rid of, every hole is filled it up with the cleaning solution after dilution, board-washing 5 times, finally buckled on clean blotting paper
It is dry.
(4) every hole is separately added into 100uL enzyme labelled antibody, and 37 DEG C incubate 20 minutes.
(5) dereaction liquid is got rid of, every hole is filled it up with the cleaning solution after dilution, board-washing 5 times, finally buckled on clean blotting paper
It is dry.
(6) every hole adds each 50 μ L of Chemoluminescent substrate A, B, is sufficiently vibrated and is uniformly mixed with micro oscillator, room temperature (20
~25 DEG C) it is protected from light 5 minutes.
(7) it must measure in adding in the after Chemoluminescent substrate the 5th~20 minute, be measured on chemiluminescence measuring instrument
The luminous intensity (RLU) in each hole, 1 second/hole of time of measuring.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of vascular endothelial growth factor chemiluminescence detection kit, which is characterized in that including reagent and hold
The kit body of reagent, the reagent include that enzyme labelled antibody, chemiluminescent substrate, concentrated cleaning solution, dilution, standard items are molten
Liquid, the section of the kit body are cylindrical structure, and the kit body includes box lower body, lid, and box lower body is upper
Open chamber is held, lid is connected through a screw thread with box lower body, and the diameter of box lower body is d2, is equipped with crash roll under box in vivo, is consolidated
Reservation, crash roll are the cylindrical structure of open upper end, and fixed seating is higher than crash roll in the upper end of fixing seat in crash roll
Upper end, fixing seat is interior to be equipped with the first blind hole, the second blind hole, the third blind hole for placing reagent bottle, and the first blind hole is located at fixing seat
Middle position, the second blind hole, third blind hole are located at the two sides of the first blind hole, hold thickening and washing respectively in the first blind hole
Liquid, dilution hold enzyme labelled antibody, chemiluminescent substrate in the second blind hole respectively, standard solution, institute are held in third blind hole
It states lid lower surface and is stained with microwell plate box, be microwell plate in microwell plate box, lid upper surface is equipped with the first groove, in the first groove
Equipped with the second groove, the section of the first groove is circular configuration, and the diameter of the first groove is cutting for d1, d1=d2, the second groove
Face is rectangle structure, and being equipped in the second groove can 90 ° of folding handle rotated.
2. a kind of vascular endothelial growth factor chemiluminescence detection kit as described in claim 1, which is characterized in that
External screw thread is equipped on the outside of the upper end of the box lower body, the inner surface lower end of lid is equipped with internal screw thread, internal screw thread and external screw thread phase
Match.
3. a kind of vascular endothelial growth factor chemiluminescence detection kit as described in claim 1, which is characterized in that
The kit body is polypropylene material.
4. a kind of vascular endothelial growth factor chemiluminescence detection kit as described in claim 1, which is characterized in that
The crash roll is sponge material.
5. a kind of vascular endothelial growth factor chemiluminescence detection kit as described in claim 1, which is characterized in that
The fixing seat is polypropylene material.
6. a kind of vascular endothelial growth factor chemiluminescence detection kit as described in claim 1, which is characterized in that
The microwell plate is polystyrene material.
7. a kind of vascular endothelial growth factor chemiluminescence detection kit as described in claim 1, which is characterized in that
The internal diameter of the lid is d3, d3=d2=d1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810872796.8A CN109061181A (en) | 2018-08-02 | 2018-08-02 | A kind of vascular endothelial growth factor chemiluminescence detection kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810872796.8A CN109061181A (en) | 2018-08-02 | 2018-08-02 | A kind of vascular endothelial growth factor chemiluminescence detection kit |
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| CN109061181A true CN109061181A (en) | 2018-12-21 |
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| CN201810872796.8A Pending CN109061181A (en) | 2018-08-02 | 2018-08-02 | A kind of vascular endothelial growth factor chemiluminescence detection kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109459421A (en) * | 2019-01-16 | 2019-03-12 | 岳昆 | A kind of serium vitamin A detection kit and its detection method |
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| CN207225983U (en) * | 2017-04-06 | 2018-04-13 | 广元棒仁食品科技股份有限公司 | A kind of packing box |
| CN207631737U (en) * | 2017-10-31 | 2018-07-20 | 河北绿色农华作物科技有限公司 | A kind of new packing structure of liquid fertilizer |
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| CN102798723A (en) * | 2012-08-03 | 2012-11-28 | 北京华创远航科技有限公司 | Chemiluminescence detection kit and preparation method |
| CN203032279U (en) * | 2013-01-25 | 2013-07-03 | 沈阳拓品文化传播有限公司 | Integrated portable type multifunctional painting box |
| CN106564692A (en) * | 2016-10-31 | 2017-04-19 | 上海纯米电子科技有限公司 | Sealing box cover for glass freshness preservation box |
| CN207062289U (en) * | 2017-03-08 | 2018-03-02 | 广州博粹医学检验科技有限公司 | A kind of ToRCH series quality-control product kit |
| CN207225983U (en) * | 2017-04-06 | 2018-04-13 | 广元棒仁食品科技股份有限公司 | A kind of packing box |
| CN207089943U (en) * | 2017-07-18 | 2018-03-13 | 南通优护优家卫生用品有限公司 | A kind of instant packed and transport washing product receiver |
| CN207631737U (en) * | 2017-10-31 | 2018-07-20 | 河北绿色农华作物科技有限公司 | A kind of new packing structure of liquid fertilizer |
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| CN109459421A (en) * | 2019-01-16 | 2019-03-12 | 岳昆 | A kind of serium vitamin A detection kit and its detection method |
| CN109459421B (en) * | 2019-01-16 | 2024-03-08 | 岳昆 | Serum vitamin A detection kit and detection method thereof |
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