CN109060995A - A kind of screening technique of almond perfume (or spice) Tea Germplasm - Google Patents
A kind of screening technique of almond perfume (or spice) Tea Germplasm Download PDFInfo
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- CN109060995A CN109060995A CN201811036799.4A CN201811036799A CN109060995A CN 109060995 A CN109060995 A CN 109060995A CN 201811036799 A CN201811036799 A CN 201811036799A CN 109060995 A CN109060995 A CN 109060995A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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- G01N30/06—Preparation
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Abstract
The invention discloses a kind of screening techniques of almond perfume (or spice) Tea Germplasm, include the following steps: to select three parts of tea tree raw materials, wherein there is " almond is fragrant " tea germplasm money two parts, another is without " almond is fragrant " tea germplasm money, weigh 50-70mg sample, it is put into grinder and grinds, after taking-up, ultrasonic extraction 20-40min；The chloroform of 100-300 μ L is added to sample, after mixing, water is added, mixes, ultrasonic extraction 10-30min；Methoxamine hydrochloride pyridine solution is added into the derivative bottle of glass, is vortexed after concussion 2min, oximation reaction is carried out in concussion and cultivate case, after taking out sample, be placed at room temperature for 30min；Collecting sample data carry out GC-MS metabonomic analysis.Through the invention technical solution can the content quickly to benzaldehyde cyanohydrin in tealeaves metabolin quickly analyzed, so as to quickly realize the screening operation of almond perfume (or spice) Tea Germplasm, greatly shortened original by the work of 4 years breeding.
Description
Technical field
The present invention relates to Tea planting technical field, specially a kind of screening technique of almond perfume (or spice) Tea Germplasm.
Background technique
" almond is fragrant " black tea is using black tea made of a kind of special tea tree Raw material processing, and made tea has almond milk
Perfume (or spice), this kind of germ plasm resource is extremely rare, and can not be recognized from the biology morphology of tea tree, therefore this kind of Tea Germplasm
It is extremely difficult to carry out breed breeding.
Current screening technique is to carry out black tea processing trial-production respectively after single plant acquires fresh leaf or single plant cutting propagation, still
Both methods workload is very big, and breakneck acceleration is relatively slow.Using the method for single plant acquisition fresh leaf processing trial-production, due to single plant tea
Dark brownish green negligible amounts are set, processing difficulties cannot keep the original characteristic of germ plasm resource, therefore cause the tea tree with certain characteristic
Germ plasm resource can not screen, and every plant of tea tree individually processes labor intensive and the time is extremely more, low efficiency, and utilize asexual cuttage
Nursery, it is breeding time needs 4 years or more, this large-scale to the method for carrying out processing trial-production after cuttage seeding cultivating and growing again
Cutting propagation may finally screen the kind that can process " almond is fragrant ", but this method from thousands of parts of Tea Germplasm
Have the shortcomings that time-consuming, at high cost, heavy workload.Therefore a kind of new technical solution is needed, apricot can be quickly filtered out
Benevolence perfume (or spice) tea tree breed, convenient for quickly planting this Rare Kinds.
Summary of the invention
The purpose of the present invention is to provide a kind of screening techniques of almond perfume (or spice) Tea Germplasm, to solve above-mentioned background skill
The problem of being proposed in art.
To achieve the above object, the invention provides the following technical scheme: a kind of screening side of almond perfume (or spice) Tea Germplasm
Method, which comprises the steps of:
S1, raw material selection: selecting three parts of tea tree raw materials, wherein two parts have " almond is fragrant " tea germplasm money, another nothing
" almond is fragrant " tea tree, as control；
S2, sample grinding: weighing 50-70mg sample, be put into grinder and grind, after taking-up, ultrasonic extraction 20-40min；
S3, sample extraction: into S2, the chloroform of 100-300 μ L is added in sample, after mixing, water is added, is mixed, ultrasound
Extract 10-30min；Methoxamine hydrochloride pyridine solution is added into the derivative bottle of glass, is vortexed after concussion 2min, is trained in concussion
It supports and carries out oximation reaction in case, after taking out sample, be placed at room temperature for 30min；
S4, data analysis: collecting sample data carry out GC-MS metabonomic analysis.
As a preferred technical solution of the present invention: it is lobate to acquire a bud two for three parts of tea tree sample standard deviations in step sl
State.
As a preferred technical solution of the present invention: the cold methanol of 360 μ L and the internal standard of 40 μ L being added in step s 2
Merging grinder is ground.
As a preferred technical solution of the present invention: temperature is in 30-40 DEG C in concussion and cultivate case in step s3, instead
90min between seasonable.
As a preferred technical solution of the present invention: in step s 4, GC-MS chromatographic condition: DB-5MS capillary column,
Carrier gas is high-purity helium, and flow velocity 1.0mL/min, the temperature of injection port is 260 DEG C, 1 μ L of sample volume, split sampling, split ratio 6:
1, solvent delay 5min.
As a preferred technical solution of the present invention: in step s 4, GC-MS Mass Spectrometry Conditions: electron impact ion source,
230 DEG C of ion source temperature, 150 DEG C of level four bars temperature, electron energy 70eV, scanning mode is full scan mode (SCAN).
As a preferred technical solution of the present invention: the helium purity is not less than 99.999%.
As a preferred technical solution of the present invention: mass scan range: m/z 50-500.
Compared with prior art, the beneficial effects of the present invention are: technical solution through the invention, it can be found that having almond
Benzaldehyde cyanohydrin rich content in its metabolin of fragrant Tea Germplasm, and benzaldehyde cyanohydrin content is less or even the tea tree breed of nothing then can not
Almond perfume is generated, and technical solution can quickly the content to benzaldehyde cyanohydrin in tealeaves metabolin carry out quickly through the invention
Analysis, so as to quickly realize the screening operation of almond perfume (or spice) Tea Germplasm, by the original work for passing through the breeding of 4 years
Greatly shorten, can accurately determine almond scented tea arborescence resource, more meet the needs of market, convenient to carry out, operation
It is convenient, it is practical, convenient for promoting the use of.
Detailed description of the invention
Fig. 1 is 1 metabolin total ion current figure of " almond is fragrant " germplasm；
Fig. 2 is 2 metabolin total ion current figure of " almond is fragrant " germplasm；
Fig. 3 is non-" almond is fragrant " germplasm metabolin total ion current figure.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment 1
The present invention provides a kind of technical solution: a kind of screening technique of almond perfume (or spice) Tea Germplasm, which is characterized in that packet
Include following steps:
S1, raw material selection: selecting three parts of tea tree raw materials, wherein two parts have " almond is fragrant " tea germplasm money, another nothing
" almond is fragrant " tea tree, acquires two leaf of a bud of 3 portions of tea trees；
S2, sample grinding: weighing 60mg sample, and the internal standard of the cold methanol of 360 μ L and 40 μ L, which are put into grinder, grinds, and takes
After out, ultrasonic extraction 30min；
S3, sample extraction: into S2, the chloroform of 200 μ L is added in sample, after mixing, water is added, is mixed, ultrasonic extraction
30min；Methoxamine hydrochloride pyridine solution is added into the derivative bottle of glass, is vortexed after concussion 2min, in 37 DEG C of shake cultures
Oximation reaction is carried out in case, reaction time 90min is being placed at room temperature for 30min after taking out sample；
S4, data analysis: collecting sample data carry out GC-MS metabonomic analysis.
In step s 4, GC-MS chromatographic condition: DB-5MS capillary column, carrier gas are high-purity helium, and helium purity is not less than
99.999%, flow velocity 1.0mL/min, the temperature of injection port are 260 DEG C, 1 μ L of sample volume, split sampling, split ratio 6:1, solvent
Postpone 5min.
In step s 4, GC-MS Mass Spectrometry Conditions: electron impact ion source, 230 DEG C of ion source temperature, level four bars temperature 150
DEG C, electron energy 70eV, scanning mode is full scan mode, mass scan range: m/z 50-500.
The metabolin for counting sample and one group of " non-almond is fragrant " sample that two groups have " almond is fragrant ", finds more than 300 kinds altogether
Metabolin wherein being turned up or turning down simultaneously in " almond is fragrant " germplasm resource, and has with non-" non-almond is fragrant " germ plasm resource and shows
Write the substance (p-value < 0.01) totally 48 of sex differernce.To above-mentioned substance, its metabolic pathway access is analyzed, discovery detection
To metabolin in there are the metabolins such as benzyl alcohol, S-MA, benzaldehyde cyanohydrin, phenylalanine to take part in aroma substance benzaldehyde jointly
Metabolic pathway, and benzaldehyde is a kind of fragrance material with almond taste, and the object of " almond is fragrant " is generated in black tea
Matter.In the metabolin for participating in forming benzaldehyde, benzaldehyde cyanohydrin is existed only in the Tea Germplasm with " almond is fragrant ", red
Benzaldehyde cyanohydrin hydrolysis generates benzaldehyde in tea process, and it is fragrant to generate almond, three groups of tealeaves its metabolin total ion current figure such as attached drawings
Shown in Fig. 1 to Fig. 3, therefore benzaldehyde cyanohydrin is the characteristic metabolic object of identification " almond is fragrant " germ plasm resource.
The Difference of Metabolism object filtered out
Technical solution through the invention, it can be found that having benzaldehyde cyanohydrin content in its metabolin of almond perfume (or spice) Tea Germplasm
It is abundant, and benzaldehyde cyanohydrin content is less or even the tea tree breed of nothing can not then generate almond perfume, and technical solution can through the invention
It is quickly analyzed with the content quickly to benzaldehyde cyanohydrin in tealeaves metabolin, so as to quickly realize almond scented tea tree
The screening operation of germ plasm resource is greatly shortened original by the work of 4 years breeding, can accurately determine almond perfume
Tea tree matter resource, more meets the needs of market, convenient to carry out, easy to operate, practical, convenient for promoting the use of.
Embodiment 2
The present invention provides another embodiment, a kind of screening technique of almond perfume (or spice) Tea Germplasm, which is characterized in that packet
Include following steps:
S1, raw material selection: selecting three parts of tea tree raw materials, wherein two parts have " almond is fragrant " tea germplasm money, another nothing
" almond is fragrant " tea tree, as control；
S2, sample grinding: weighing 50mg sample, be put into grinder and grind, after taking-up, ultrasonic extraction 20min；
S3, sample extraction: into S2, the chloroform of 100 μ L is added in sample, after mixing, water is added, is mixed, ultrasonic extraction
10min；Methoxamine hydrochloride pyridine solution is added into the derivative bottle of glass, is vortexed after concussion 2min, in concussion and cultivate case
Oximation reaction is carried out, after taking out sample, is being placed at room temperature for 30min；
S4, data analysis: collecting sample data carry out GC-MS metabonomic analysis.
Three parts of tea tree sample standard deviations acquire two leaf state of a bud in step sl；The cold methanol of 360 μ L is added in step s 2
It is ground with the internal standard merging grinder of 40 μ L；Temperature is in 30-40 DEG C in concussion and cultivate case in step S3, the reaction time
90min。
In step s 4, GC-MS chromatographic condition: DB-5MS capillary column, carrier gas are high-purity helium, flow velocity 1.0mL/min,
The temperature of injection port is 260 DEG C, 1 μ L of sample volume, split sampling, split ratio 6:1, solvent delay 5min；Mass Spectrometry Conditions: electronics bangs
Ion source is hit, 230 DEG C of ion source temperature, 150 DEG C of level four bars temperature, electron energy 70eV, scanning mode is full scan mode.
Embodiment 3
A kind of screening technique of almond perfume (or spice) Tea Germplasm, which comprises the steps of:
S1, raw material selection: selecting three parts of tea tree raw materials, wherein two parts have " almond is fragrant " tea germplasm money, another nothing
" almond is fragrant " tea tree, as control；
S2, sample grinding: weighing 70mg sample, be put into grinder and grind, after taking-up, ultrasonic extraction 40min；
S3, sample extraction: into S2, the chloroform of 300 μ L is added in sample, after mixing, water is added, is mixed, ultrasonic extraction
130min；Methoxamine hydrochloride pyridine solution is added into the derivative bottle of glass, is vortexed after concussion 2min, in concussion and cultivate case
Oximation reaction is carried out, after taking out sample, is being placed at room temperature for 30min；
S4, data analysis: collecting sample data carry out GC-MS metabonomic analysis.
Three parts of tea tree sample standard deviations acquire two leaf state of a bud in step sl；The cold methanol of 360 μ L is added in step s 2
It is ground with the internal standard merging grinder of 40 μ L；Temperature is in 30-40 DEG C in concussion and cultivate case in step S3, the reaction time
90min。
In step s 4, GC-MS chromatographic condition: DB-5MS capillary column, carrier gas are high-purity helium, flow velocity 1.0mL/min,
The temperature of injection port is 260 DEG C, 1 μ L of sample volume, split sampling, split ratio 6:1, solvent delay 5min；Mass Spectrometry Conditions: electronics bangs
Ion source is hit, 230 DEG C of ion source temperature, 150 DEG C of level four bars temperature, electron energy 70eV, scanning mode is full scan mode.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of screening technique of almond perfume (or spice) Tea Germplasm, which comprises the steps of:
S1, raw material selection: selecting three parts of tea tree raw materials, wherein two parts have " almond is fragrant " tea germplasm money, another is without " almond
It is fragrant " tea germplasm money, as control；
S2, sample grinding: weighing 50-70mg sample, be put into grinder and grind, after taking-up, ultrasonic extraction 20-40min；
S3, sample extraction: into S2, the chloroform of 100-300 μ L is added in sample, after mixing, water is added, is mixed, ultrasonic extraction
10-30min；Methoxamine hydrochloride pyridine solution is added into the derivative bottle of glass, is vortexed after concussion 2min, in concussion and cultivate case
Middle carry out oximation reaction is being placed at room temperature for 30min after taking out sample；
S4, data analysis: collecting sample data carry out GC-MS metabonomic analysis.
2. a kind of screening technique of almond perfume (or spice) Tea Germplasm according to claim 1, it is characterised in that: in step S1
In three parts of tea tree sample standard deviations acquire two leaf states of a bud.
3. a kind of screening technique of almond perfume (or spice) Tea Germplasm according to claim 1, it is characterised in that: in step S2
The internal standard merging grinder of the middle cold methanol that 360 μ L are added and 40 μ L are ground.
4. a kind of screening technique of almond perfume (or spice) Tea Germplasm according to claim 1, it is characterised in that: in step S3
Temperature is in 30-40 DEG C in middle concussion and cultivate case, reaction time 90min.
5. a kind of screening technique of almond perfume (or spice) Tea Germplasm according to claim 1, it is characterised in that: in step S4
In, GC-MS chromatographic condition: DB-5MS capillary column, carrier gas are high-purity helium, and the temperature of flow velocity 1.0mL/min, injection port are
260 DEG C, 1 μ L of sample volume, split sampling, split ratio 6:1, solvent delay 5min.
6. a kind of screening technique of almond perfume (or spice) Tea Germplasm according to claim 1, it is characterised in that: in step S4
In, GC-MS Mass Spectrometry Conditions: electron impact ion source, 230 DEG C of ion source temperature, 150 DEG C of level four bars temperature, electron energy 70eV,
Scanning mode is full scan mode.
7. a kind of screening technique of almond perfume (or spice) Tea Germplasm according to claim 5, it is characterised in that: the helium
Purity is not less than 99.999%.
8. a kind of screening technique of almond perfume (or spice) Tea Germplasm according to claim 6, it is characterised in that: mass scanning
Range: m/z 50-500.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109632999A (en) * | 2018-12-24 | 2019-04-16 | 广东省农业科学院茶叶研究所 | The sprout period authenticating method of " almond is fragrant " black tea resource |
| CN110892864A (en) * | 2019-12-03 | 2020-03-20 | 海南农垦大丰咖啡产业集团有限公司 | Asexual high-yield coffee breeding method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105866299A (en) * | 2016-03-31 | 2016-08-17 | 上海青鹿投资有限公司 | GC-MS-based plant non-targeted metabolomics sample pretreatment method |
| CN106290617A (en) * | 2016-07-29 | 2017-01-04 | 云南省烟草农业科学研究院 | A kind of Nicotiana tabacum L. metabonomic analysis methods based on gaschromatographic mass spectrometry |
-
2018
- 2018-09-06 CN CN201811036799.4A patent/CN109060995B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105866299A (en) * | 2016-03-31 | 2016-08-17 | 上海青鹿投资有限公司 | GC-MS-based plant non-targeted metabolomics sample pretreatment method |
| CN106290617A (en) * | 2016-07-29 | 2017-01-04 | 云南省烟草农业科学研究院 | A kind of Nicotiana tabacum L. metabonomic analysis methods based on gaschromatographic mass spectrometry |
Non-Patent Citations (8)
| Title |
|---|
| CHI-TANG HO等: "Tea aroma formation", 《FOOD SCIENCE AND HUMAN WELLNESS》 * |
| WENFEI GUO等: "Isolation of An Aroma Precursor of Benzaldehyde from Tea Leaves", 《BIOSCI. BIOTECHNOL. BIOCHEM.》 * |
| ZIYIN YANG等: "Recent studies of the volatile compounds in tea", 《FOOD RESEARCH INTERNATIONAL》 * |
| 任洪涛等: "不同级别云南工夫红茶香气成分比较分析", 《南方农业学报》 * |
| 兰欣等: "ＨＳ－ＳＰＭＥ法结合ＧＣ－ＭＳ分析崂山绿茶的香气成分", 《食品与机械》 * |
| 周春娟等: "不同品种（系）凤凰单丛成品茶的香型分类与鉴定", 《茶叶科学》 * |
| 王秋霜等: "广东罗坑野生茶树群落红茶香气物质鉴析", 《中国农学通报》 * |
| 谭超等: "三种工夫红茶挥发性成分同异性分析", 《蚕桑茶叶通讯》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109632999A (en) * | 2018-12-24 | 2019-04-16 | 广东省农业科学院茶叶研究所 | The sprout period authenticating method of " almond is fragrant " black tea resource |
| CN110892864A (en) * | 2019-12-03 | 2020-03-20 | 海南农垦大丰咖啡产业集团有限公司 | Asexual high-yield coffee breeding method |
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