CN109055485A - A kind of sdLDL-C assay kit - Google Patents

A kind of sdLDL-C assay kit Download PDF

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Publication number
CN109055485A
CN109055485A CN201810844628.8A CN201810844628A CN109055485A CN 109055485 A CN109055485 A CN 109055485A CN 201810844628 A CN201810844628 A CN 201810844628A CN 109055485 A CN109055485 A CN 109055485A
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China
Prior art keywords
sdldl
reagent
assay kit
solvent
purified water
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Pending
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CN201810844628.8A
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Chinese (zh)
Inventor
黄信用
吕崇翔
曹春梅
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JINHUA KINGS BIOLOGICAL TECHNOLOGY Co Ltd
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JINHUA KINGS BIOLOGICAL TECHNOLOGY Co Ltd
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Priority to CN201810844628.8A priority Critical patent/CN109055485A/en
Publication of CN109055485A publication Critical patent/CN109055485A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/30—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving catalase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

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  • Chemical & Material Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of sdLDL-C assay kits, including two kinds of independent liquid reagents of R1 and R2, each constituent concentration of R1 reagent are as follows: Good ' s buffer 100mmol/L, cholesterol esterase (CHE) 1600U/L, cholesterol oxidase (CO) 600U/L, phosphatidase 2700U/L, hydrogen peroxidase 12 00KU/L, N- ethyl-N- (2-Hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS) 2mmol/L, solvent are purified water；Each constituent concentration of R2 reagent are as follows: Good ' s buffer 100mmol/L, peroxidase (POD) 5000U/L, 4-AA 4mmol/L, solvent are purified water.The present invention can be suitble to clinical labororatory to the needs of sdLDL-C assay.

Description

A kind of sdLDL-C assay kit
Technical field
The invention belongs to serum detection reagent technical fields more particularly to a kind of sdLDL-C to survey Determine kit.
Background technique
Lipoprotein in blood can be divided into high-density lipoprotein (HDL), low-density lipoprotein according to its density size (LDL), four major class of very low density lipoprotein (VLDL) and chylomicron (CM).Different types of lipoprotein cholesterol is for causing The ability of atherosclerosis is different.Usually clinically detecting most components is high-density lipoprotein cholesterol (HDL-C), Low density lipoprotein cholesterol (LDL-C), conventionally, high-density lipoprotein cholesterol has arteries certain guarantor The risk of cardiovascular disease occurs for shield effect, content height with regard to low, and low density lipoprotein cholesterol then easily causes Atherosclerosis Change, increases the risk that cardiovascular disease occurs.However, there are groups of people on nearest research surface even if the content of LDL-C is lower Group is also easy to happen cardiovascular disease, and cardiovascular disease can not necessarily occur for the part higher crowd of LDL-C content.This can Can be related with the inhomogeneity of LDL-C, there is the smaller density of particle in the subfraction of studies have shown that low-density lipoprotein biggish LDL is known as small dense low-density lipoprotein (small dense Low-Density Lipoprotein, abbreviation sdLDL)；And incite somebody to action Particle is larger, the lower LDL of density is known as big and light LDL.In contrast, the ability of sdLDL atharosclerosis is stronger, is One of the significant risk factor of initiation cardiovascular disease generally acknowledged at present.
In current detection method, the method for measuring sdLDL mainly has electrophoresis, supercentrifugation, precipitation classification etc.. But the step of electrophoresis technique determining, is cumbersome, and preci-sion and accuracy is all to be improved, clinical application is not strong；Ultracentrifugation Method is to measure the standard method of lipoprotein, but need ultracentrifuge with method detection sdLDL, and detection flux is not high, Requirement to operator is high, therefore the method is not also promoted clinically well；Although precipitation classification to operate It is upper relatively easy, but still settling step is needed, the preci-sion and accuracy of the method is all relatively poor, is not suitable for clinical application. Thus it is badly in need of the simple and quick method of one kind and is able to detect sdLDL or sdLDL-C.
Summary of the invention
The object of the invention seeks to adapt to clinical labororatory to the need of sdLDL-C assay It wants, a kind of kit for serum small and dense low-density lipoprotein cholesterol determination is provided.
To achieve the above object, the present invention provides the following technical scheme that
A kind of sdLDL-C assay kit, including reagent R1 independent of each other and reagent R2 are bis- Liquid component, including ingredient and corresponding content are as follows:
Reagent R1:
Buffer 100mmol/L
Cholesterol esterase (CHE) 1600U/L
Cholesterol oxidase (CO) 600U/L
Phosphatidase 2700U/L
Hydrogen peroxidase 12 00KU/L
N- ethyl-N- (2-Hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS) 2mmol/L
Surfactant 5g/L
Preservative 0.7g/L
Its solvent is purified water
Reagent R2:
Buffer 100mmol/L
Peroxidase (POD) 5000U/L
4-AA 4mmol/L
Surfactant 5g/L
Preservative 0.7g/L
Its solvent is purified water.
Preferably, the buffer is Good ' s buffer.
Preferably, the surfactant is one of TWEEN 20, EMULGEN120 and TX100.
Preferably, the preservative is at least one of Sodium azide, thimerosal, macrolide antibiotics.
By using above-mentioned technical proposal, the invention has the following advantages: being able to use automatic clinical chemistry analyzer Serum small and dense low-density lipoprotein cholesterol capacity is directly measured, is suitble to clinical labororatory to small dense low-density lipoprotein gallbladder The needs of sterol assay.
Specific embodiment
In order to make those skilled in the art that the present invention may be better understood, below with reference to embodiment to the technology of the present invention Scheme further illustrates.
A kind of sdLDL-C assay kit, including reagent R1 independent of each other and reagent R2 are bis- Liquid component, including ingredient and corresponding content are as follows:
Reagent R1:
Buffer 100mmol/L
Cholesterol esterase (CHE) 1600U/L
Cholesterol oxidase (CO) 600U/L
Phosphatidase 2700U/L
Hydrogen peroxidase 12 00KU/L
N- ethyl-N- (2-Hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS) 2mmol/L
Surfactant 5g/L
Preservative 0.7g/L
Its solvent is purified water
Reagent R2:
Buffer 100mmol/L
Peroxidase (POD) 5000U/L
4-AA 4mmol/L
Surfactant 5g/L
Preservative 0.7g/L
Its solvent is purified water.
Preferably, the buffer is Good ' s buffer.
Preferably, the surfactant is one of TWEEN 20, EMULGEN120 and TX100.
Preferably, the preservative is at least one of Sodium azide, thimerosal, macrolide antibiotics.
The preferred embodiment of the present invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art For, without departing from the inventive concept of the premise, several deformations can also be made, improves and substitutes, these belong to this hair Bright protection scope.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.

Claims (4)

1. a kind of sdLDL-C assay kit, which is characterized in that including reagent R1 independent of each other With reagent R2 biliquid component, including ingredient and corresponding content are as follows:
Reagent R1:
Buffer 100mmol/L
Cholesterol esterase (CHE) 1600U/L
Cholesterol oxidase (CO) 600U/L
Phosphatidase 2700U/L
Hydrogen peroxidase 12 00KU/L
N- ethyl-N- (2-Hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS) 2mmol/L
Surfactant 5g/L
Preservative 0.7g/L
Its solvent is purified water
Reagent R2:
Buffer 100mmol/L
Peroxidase (POD) 5000U/L
4-AA 4mmol/L
Surfactant 5g/L
Preservative 0.7g/L
Its solvent is purified water.
2. sdLDL-C assay kit as described in claim 1, which is characterized in that the buffering Liquid is Good ' s buffer.
3. sdLDL-C assay kit as described in claim 1, which is characterized in that the surface Activating agent is one of TWEEN 20, EMULGEN120 and TX100.
4. sdLDL-C assay kit as described in claim 1, which is characterized in that the anti-corrosion Agent is at least one of Sodium azide, thimerosal, macrolide antibiotics.
CN201810844628.8A 2018-07-27 2018-07-27 A kind of sdLDL-C assay kit Pending CN109055485A (en)

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Application Number Priority Date Filing Date Title
CN201810844628.8A CN109055485A (en) 2018-07-27 2018-07-27 A kind of sdLDL-C assay kit

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CN109055485A true CN109055485A (en) 2018-12-21

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109609595A (en) * 2019-01-24 2019-04-12 浙江夸克生物科技有限公司 A kind of sdLDL-C assay kit and its application
CN110160974A (en) * 2019-05-28 2019-08-23 天津悦和康生物技术有限公司 A kind of kit detecting sdLDL-C
CN110564810A (en) * 2019-09-19 2019-12-13 武汉瀚海新酶生物科技有限公司 High-performance small and dense low-density lipoprotein cholesterol detection kit
CN111269962A (en) * 2020-03-18 2020-06-12 浙江夸克生物科技有限公司 Small and dense low-density lipoprotein cholesterol determination kit and application thereof
CN111690715A (en) * 2019-03-12 2020-09-22 程明 Small and dense low-density lipoprotein cholesterol determination kit and determination method thereof
CN112941144A (en) * 2021-03-01 2021-06-11 江西英大生物技术有限公司 Small and dense low-density lipoprotein cholesterol determination kit
JP7437263B2 (en) 2020-07-31 2024-02-22 デンカ株式会社 Reagent compositions and kits

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103589776A (en) * 2013-09-12 2014-02-19 绍兴圣康生物科技有限公司 Small and dense low-density lipoprotein cholesterol determination kit
CN104678111A (en) * 2015-02-10 2015-06-03 浙江凯成生物科技有限公司 Method, reagent and kit for quantitatively detecting low-density lipoprotein cholesterol
CN107446989A (en) * 2017-06-23 2017-12-08 美康生物科技股份有限公司 Determine the method and reagent of the sdLDL-C in sample
CN107505272A (en) * 2017-08-10 2017-12-22 威特曼生物科技（南京）有限公司 LDL-C detection kit and its application method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103589776A (en) * 2013-09-12 2014-02-19 绍兴圣康生物科技有限公司 Small and dense low-density lipoprotein cholesterol determination kit
CN104678111A (en) * 2015-02-10 2015-06-03 浙江凯成生物科技有限公司 Method, reagent and kit for quantitatively detecting low-density lipoprotein cholesterol
CN107446989A (en) * 2017-06-23 2017-12-08 美康生物科技股份有限公司 Determine the method and reagent of the sdLDL-C in sample
CN107505272A (en) * 2017-08-10 2017-12-22 威特曼生物科技（南京）有限公司 LDL-C detection kit and its application method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WANG,L等: "The detection of serum sdLDL-C in the CAD patients and clinical application", 《HEART》 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109609595A (en) * 2019-01-24 2019-04-12 浙江夸克生物科技有限公司 A kind of sdLDL-C assay kit and its application
CN111690715A (en) * 2019-03-12 2020-09-22 程明 Small and dense low-density lipoprotein cholesterol determination kit and determination method thereof
CN110160974A (en) * 2019-05-28 2019-08-23 天津悦和康生物技术有限公司 A kind of kit detecting sdLDL-C
CN110564810A (en) * 2019-09-19 2019-12-13 武汉瀚海新酶生物科技有限公司 High-performance small and dense low-density lipoprotein cholesterol detection kit
CN111269962A (en) * 2020-03-18 2020-06-12 浙江夸克生物科技有限公司 Small and dense low-density lipoprotein cholesterol determination kit and application thereof
CN111269962B (en) * 2020-03-18 2021-03-23 浙江夸克生物科技有限公司 Small and dense low-density lipoprotein cholesterol determination kit and application thereof
JP7437263B2 (en) 2020-07-31 2024-02-22 デンカ株式会社 Reagent compositions and kits
CN112941144A (en) * 2021-03-01 2021-06-11 江西英大生物技术有限公司 Small and dense low-density lipoprotein cholesterol determination kit

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