CN109046487B - Quantitative liquid taking device for measuring liquid density - Google Patents
Quantitative liquid taking device for measuring liquid density Download PDFInfo
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- CN109046487B CN109046487B CN201810652043.6A CN201810652043A CN109046487B CN 109046487 B CN109046487 B CN 109046487B CN 201810652043 A CN201810652043 A CN 201810652043A CN 109046487 B CN109046487 B CN 109046487B
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
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- G—PHYSICS
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- G01N9/00—Investigating density or specific gravity of materials; Analysing materials by determining density or specific gravity
- G01N9/02—Investigating density or specific gravity of materials; Analysing materials by determining density or specific gravity by measuring weight of a known volume
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Abstract
The invention provides a quantitative liquid taking device for measuring liquid density, which comprises: a micro-quantitative container, which comprises a main body forming a certain volume and openings positioned at two ends of the main body, can contain a certain volume of liquid and keep the liquid therein to weigh the whole mass; the liquid taking device comprises a cylindrical component, a piston component which can slide in the cylindrical component, and a flexible tubular component which is arranged at a liquid inlet/outlet of the cylindrical component and is communicated with the fluid in the cylindrical component; the side wall of the cylindrical part is provided with a hole which can communicate the inside of the cylindrical part with the outside fluid; the micro quantitative container can be detachably connected with the liquid taking device and forms fluid communication, so that the micro quantitative container extracts or discharges a certain volume of liquid.
Description
Technical Field
The invention relates to a quantitative liquid taking device for measuring liquid density, in particular to a quantitative liquid taking device for measuring the liquid density when the sample volume is less than 1mL, and belongs to the field of physical measurement of liquid materials.
Background
In the detection and inspection process, the density of an unknown solution is often required to be accurately measured, sometimes the sample amount is small (1-2 ml), and the information of the sample required to be measured is large.
One of the commonly used density measuring devices is to determine the density of a liquid by measuring the weight of a known volume of liquid.
The current commercially available density measuring equipment also has a minimum sample size of 1ml with an accuracy of 0.001g/cm3 (e.g., Mettler-Torledo DA-100M digital densitometer). If the density is measured by a pipette, the error is generally about 2%.
Accordingly, there is a need in the art for a device and method that can accurately determine the density of a liquid when the sample volume is small.
Disclosure of Invention
The invention aims to provide a device and a method capable of accurately measuring the density of liquid under the condition that the sample amount is small.
The technical scheme of the invention is as follows.
One aspect of the present invention provides a quantitative liquid taking device for measuring density of a liquid, comprising:
a micro-quantitative container including a body forming a certain volume and openings at both ends of the body, capable of containing a certain volume of liquid and holding the liquid therein to weigh the whole mass, the opening at least one end of the body being formed by a first capillary;
the liquid taking device comprises a cylindrical component, a piston component which can slide in the cylindrical component, and a flexible tubular component which is arranged at a liquid inlet/outlet of the cylindrical component and is communicated with the fluid in the cylindrical component; the side wall of the cylindrical part is provided with a hole which can communicate the inside of the cylindrical part with the outside fluid;
the micro quantitative container can be detachably connected with the liquid taking device and forms fluid communication, so that the micro quantitative container extracts or discharges a certain volume of liquid.
Preferably, the micro quantitative container is a micro quantitative tube.
Preferably, the micro quantitative tube is made of a hydrophobic transparent material.
Preferably, the body is provided with a second capillary between the openings at both ends.
Preferably, the body comprises a body capillary having an inner diameter greater than the inner diameters of the first and second capillaries.
Preferably, the inner diameter of the main capillary tube is 0.5-6.0 mm, and the length of the main capillary tube is 10-100 mm.
Preferably, the inner diameter of the first fine capillary is less than 0.5mm, and the length of the first fine capillary is 1-20 mm; the second capillary has an inner diameter of less than 0.5mm and a length of about 10 mm.
Preferably, the cylindrical member has an inner diameter of less than 10mm and a length of less than 85 mm.
The invention provides a method for using the quantitative liquid taking device for measuring the density of the liquid according to one of the technical schemes, which comprises the following steps:
connecting one end of a micro quantitative container of the quantitative liquid taking device with a flexible tubular part of the liquid taking device to form fluid communication;
vertically inserting the quantitative liquid taking device into the constant-temperature liquid to be taken, sealing a hole in the side wall of the cylindrical part of the liquid taking device, pulling the piston part, sucking the liquid to be taken into the micro quantitative container, and stopping pulling the piston when liquid beads overflow from one end of the micro quantitative container;
and taking the quantitative liquid taking device out of the liquid to be taken, horizontally placing and balancing, opening the hole, and rotating the liquid taking device to separate the liquid taking device from the trace quantitative container.
Preferably, the method further comprises the following steps:
when the liquid in the micro quantitative container is discharged, one end of the micro quantitative container of the quantitative liquid taking device is connected with the flexible tubular part of the liquid taking device to form fluid communication, the flexible tubular part is vertically placed to seal the hole on the wall of the liquid taking device, the piston part is pushed, and the liquid in the micro quantitative container is emptied.
Through the technical scheme, the invention can obtain the following technical effects.
The micro quantitative tube is made of hydrophobic transparent materials such as glass, quartz, organic glass and the like which are not easy to absorb liquid, so that the measurement error is reduced. Meanwhile, the mass of the micro quantitative tube is lighter than the mass of the loaded sample, and the weighing sensitivity of the analytical balance is not influenced. The inner diameter of the micro quantitative tube is thinner than 0.5mm after the two ends are hot-melted and stretched, and the error caused by the concave liquid surfaces at the two ends is greatly reduced. The overall length of the micro quantitative tube is less than 100mm, so that the micro quantitative tube can be conveniently placed on a tray of an analytical balance.
When the liquid taking device takes liquid, the constant pressure hole is blocked to pull the piston, and negative pressure generated in the liquid taking device cavity provides power for lifting the liquid for the trace quantitative tube, so that the trace quantitative tube is conveniently filled with the liquid. After the solution is taken out, the constant pressure hole is blocked, and the micro quantitative tube is taken out from the solution and placed horizontally. The constant pressure hole is blocked in the process, and the cavity of the liquid taking device is sealed at the moment, so that the liquid in the trace quantitative tube can be prevented from flowing out from the bottom due to the action of gravity. After the micro quantitative tube is horizontally placed, the constant pressure hole is loosened, and the liquid taking device is slowly rotated to be separated from the micro quantitative tube. At the moment, the pressure of the liquid taking device cavity is communicated with the outside, so that negative pressure is prevented from being generated in the liquid taking device cavity when the liquid taking device cavity is separated from the trace quantitative tube, and the liquid in the trace quantitative tube is sucked out. After weighing is finished, the constant pressure hole is blocked, the piston is pushed, positive pressure is generated in the liquid taking device cavity, and liquid in the trace quantitative tube can be discharged out of the trace quantitative tube.
Drawings
FIG. 1 is a schematic view of a micro-volume measuring container of the quantitative liquid-dispensing device of the present invention.
Fig. 2 is a schematic view of a liquid dispenser of the quantitative liquid dispenser of the present invention.
Detailed Description
Example 1
As shown in fig. 1, the present embodiment provides a micro-quantitative container, which includes a body forming a certain volume and openings at both ends of the body, and is capable of containing a certain volume of liquid and holding the liquid therein to weigh the whole mass; wherein the opening of at least one end of the body is formed by a first capillary T1.
Preferably, the main body T0 is provided with a second capillary T2 between openings at both ends.
Preferably, the body includes a body capillary T0, the body capillary T0 having an inner diameter greater than the inner diameters of the first and second capillaries T1, T2.
In a preferred embodiment, as shown in fig. 1(a), both end openings of the body are formed by first capillaries T1; the inner diameter of the main capillary T0 is 0.5-6 mm, and the length is 10-100 mm; the inner diameter of the first capillary T1 is less than 0.5mm, and the length is 1-20 mm.
As shown in fig. 1(b), in a preferred embodiment, the opening of only one end of the body is formed by a first capillary T1; and a second capillary T2 is provided at a distance from the opening forming the first capillary T1. In a preferred embodiment, the second capillary is spaced about 80mm from the opening forming the first capillary. The inner diameter of the main capillary T0 is 0.5-6 mm, and the length is 10-100 mm; the inner diameter of the first capillary T1 is less than 0.5mm, and the length is 1-20 mm; the second capillary T2 has an inner diameter of less than 0.5mm and a length of about 10 mm.
As can be understood by those skilled in the art, the inner diameter of the micro quantitative tube after hot melting and stretching at two ends is very thin and less than 0.5mm, and the error caused by the concave liquid level at two ends is greatly reduced. The overall length of the micro quantitative tube is less than 100mm, so that the micro quantitative tube can be conveniently placed on a tray of an analytical balance.
Preferably, the second capillary T2 is about 80mm from the opening forming the first capillary T1.
Preferably, the micro quantity dosing container is made of a hydrophobic transparent material.
Preferably, the hydrophobic transparent material comprises glass, quartz, organic glass.
As can be understood by those skilled in the art, the micro quantitative tube is made of hydrophobic transparent materials such as glass, quartz, organic glass and the like which are not easy to absorb liquid, so that the measurement error can be reduced. Meanwhile, whether air bubbles exist in the tube can be easily checked so as to eliminate the air bubbles in time.
Example 2
The embodiment provides a quantitative liquid taking device for measuring liquid density, which comprises:
a micro-metering container comprising a body defining a volume and openings at opposite ends of the body capable of holding a volume of liquid and holding the liquid therein for weighing the entire mass, as shown in fig. 1.
The liquid taking device comprises a cylindrical component 10, a piston component 20 which can slide in the cylindrical component, and a flexible tubular component 30 which is arranged at a liquid inlet/outlet of the cylindrical component and is communicated with the inside of the cylindrical component in a fluid mode; the side wall of the tubular member is provided with a hole 40 which enables fluid communication between the interior of the tubular member and the exterior, as shown in figure 2.
The micro quantitative container can be detachably connected with the liquid taking device and forms fluid communication, so that the micro quantitative container extracts or discharges a certain volume of liquid.
Preferably, the micro quantitative container is a micro quantitative tube.
Preferably, the micro quantitative tube is made of a hydrophobic transparent material.
Preferably, the micro quantitative tube comprises a main capillary tube and a fine capillary tube connected to two ends of the main capillary tube, and the inner diameter of the fine capillary tube is smaller than that of the main capillary tube.
Preferably, the micro quantitative tube comprises a main capillary, a fine capillary connected to one end of the main capillary, and a fine capillary formed in the middle of the main capillary; the inner diameter of the fine capillary is smaller than that of the main capillary.
Preferably, the inner diameter of the main capillary tube is 0.5-6.0 mm, and the length of the main capillary tube is 10-100 mm.
Preferably, the inner diameter of the fine capillary is less than 0.5mm, and the length of the fine capillary is 1-20 mm.
Preferably, the cylindrical member 10 has an inner diameter of less than 10mm and a length of less than 85 mm.
Preferably, the cylindrical member 10 is made of a transparent or translucent material.
Preferably, the flexible tubular member 30 is a latex tube having a diameter of about 1mm and a length of 10 mm.
Example 3
Another aspect of the present invention provides a method for using a quantitative liquid taking device for measuring liquid density according to one of the above technical solutions, comprising the steps of:
connecting one end of a micro quantitative container of the quantitative liquid taking device with a flexible tubular part of the liquid taking device to form fluid communication;
vertically inserting the quantitative liquid taking device into the constant-temperature liquid to be taken, sealing a hole in the side wall of the cylindrical part of the liquid taking device, pulling the piston part, sucking the liquid to be taken into the micro quantitative container, and stopping pulling the piston when liquid beads overflow from one end of the micro quantitative container;
and taking the quantitative liquid taking device out of the liquid to be taken, horizontally placing and balancing, opening the hole, and rotating the liquid taking device to separate the liquid taking device from the trace quantitative container.
Preferably, the method further comprises the steps of:
when the liquid in the micro quantitative container is discharged, one end of the micro quantitative container of the quantitative liquid taking device is connected with the flexible tubular part of the liquid taking device to form fluid communication, the hole on the wall of the liquid taking device is closed and vertically placed, the piston part is pushed, and the liquid in the micro quantitative container is emptied.
Example 4
The invention also provides a method for measuring the liquid density, which comprises the following steps:
and step S1, controlling the temperature of the operation room to be at a preset temperature, and providing a reference liquid and a liquid to be measured, wherein the reference liquid can be provided in a sufficient amount, and the liquid to be measured is below 2mL, and the reference liquid and the liquid to be measured are placed together in the operation room for a specified time.
S2, shaking up the liquid to be measured and dispersing the bubbles in the liquid;
step S3, a mass measuring device and a micro quantitative container are provided.
Step S4, measuring the mass m of the micro quantitative container by using the mass measuring device0。
In step S5, the operation in step S4 is repeated a plurality of times.
And step S6, inserting one end of the micro quantitative container into the head end of the latex of the liquid extractor and penetrating the latex head by about 3 mm. The device is vertically inserted into a constant-temperature reference liquid, a constant-pressure hole on the wall of the liquid taking device is blocked by a finger, a piston of the liquid taking device is pulled, ultrapure water is sucked into a micro quantitative tube, and the piston is stopped being pulled when liquid beads overflow from the upper end. And (3) slightly taking the device out of the reference liquid, horizontally placing and balancing, slightly releasing the constant-pressure hole, and slowly rotating the liquid taking device to separate the liquid taking device from the micro quantitative container. Wiping the outer wall of the micro-dosing container with a non-dusting paperAnd (6) residual liquid. The micro-dosing container is checked when the tube is filled with a reference liquid without air bubbles. Placing the micro quantitative container on the mark point of the mass measuring device, wherein the mass m of the micro quantitative container after being filled with the reference liquid1. Then the device is taken out of the quality measuring device, a constant pressure hole on the wall of the liquid taking device is blocked by a finger, the device is vertically placed above the waste liquid cup, and the piston of the liquid taking device is pushed to empty the liquid in the trace quantitative tube.
In step S7, the operation in step S6 is repeated a plurality of times.
And step S8, filling a certain volume of liquid to be measured into the micro quantitative container, fully rinsing the micro quantitative tube, and then emptying.
In step S9, the operation in step S8 is repeated a plurality of times to sufficiently rinse the micro quantitative container.
Step S10, filling the liquid to be measured into the micro quantitative container, and measuring the mass m of the micro quantitative container filled with the liquid to be measured by using the mass measuring device2(ii) a Then the liquid to be measured in the micro quantitative container is emptied.
In step S11, the operation in step S10 is repeated a plurality of times.
Step S12, according to the reference liquid density D at the preset temperature0And the mass average value of the micro quantitative container calculated in the steps S5 to S7
Average mass value of reference liquid filled in micro quantitative container
Calculating the volume of the micro quantitative container
Step 13, according to the average mass value of the micro quantitative container filled with the liquid to be measured in the steps S10 and S11
Calculating the solution to be measuredDensity of the body
Preferably, the predetermined temperature in the step S1 is 20 ℃ ± 0.5 ℃.
Preferably, the specified time in step S1 is 2 hours or more.
Preferably, the amount of the liquid to be measured in the step S1 is 0.5-2 mL.
Preferably, the mass measuring device is a balance.
Preferably, the micro dosing container is placed on the scale at a marked point on the tray of the scale when measuring mass, such that the center of gravity of the micro dosing container is at the center of the tray of the scale.
Preferably, the operation in step S4 is repeated 3 or more times in step S5; the operation in step S6 is repeated 3 times or more in the step S7; the operation in S8 is repeated 2 or more times in the step S9; the operation in step S10 is repeated 3 times or more in the step S11.
Preferably, the volume of the micro quantitative container is less than 1 mL.
Preferably, the micro quantitative container is a micro quantitative tube.
The liquid density measuring method of the present invention will be described below with reference to specific applications.
The apparatus used was:
the liquid density measuring device comprises a trace quantitative tube and a liquid taking device;
analytical balance (XPE205, d 0.01mg, Mettler, germany);
a digital hygrothermograph;
1 piece of 50ml centrifuge tube (CORNING);
1 test tube rack;
dust free paper (KIMTECH)1 boxes;
the powder-free latex gloves 2 are double;
the reagents used were:
1mL of sample to be detected;
GBW (E)090623 calcium standards in frozen human serum;
ultrapure water, Milli-Q A10 ultrapure water machine (llipore corporation, USA).
Balance room environmental requirements:
the balance room should be free of direct sunlight;
the balance workbench is firm and reliable, and the levelness of the table top is good;
the working chamber is clean, so that the influence of air flow is avoided;
the temperature in the working chamber is constant and can be controlled to be 20 +/-0.5 ℃;
it should be far away from the environments of vibration source, high-energy heat source, high-strength electromagnetic field, etc.
The method comprises the following operation steps:
step 1: the temperature of the operating room is controlled to 20 +/-0.5 ℃, 40ml of ultrapure water and GBW (E)090623 calcium standard substance in frozen human serum are put in a centrifuge tube to be placed for more than 2 hours in a constant temperature operating room.
Step 2: the standard substance was shaken well and allowed to stand for 1 hour to disperse the bubbles in the solution.
And step 3: two points are marked transversely on the tray of the analytical balance with a marker pen, and when the micro dosing tube is placed on the two points, the center of gravity is exactly at the center of the balance tray.
And 4, step 4: and placing the micro quantitative tube on a mark point of an analytical balance, and reading and recording the mass of the micro quantitative tube.
And 5: the operation in step 4 was repeated 6 times, and the mass of the microtube was recorded as in Table 1.
Step 6: one end of the micro quantitative tube is inserted into the head end of the latex of the liquid taking device and penetrates through the latex head by about 3 mm. The device is vertically inserted into constant-temperature ultrapure water, a constant-pressure hole on the wall of the liquid taking device is blocked by a finger, a piston of the liquid taking device is pulled, the ultrapure water is sucked into the micro-quantitative tube, and the piston is stopped being pulled when liquid beads overflow from the upper end. And (3) lightly taking the device out of the ultrapure water, horizontally placing and balancing, then lightly releasing the constant-pressure hole, and slowly rotating the liquid taking device to separate the liquid taking device from the trace quantitative tube. And wiping residual liquid on the outer wall of the micro quantitative tube by using dust-free paper. The micro quantitative tube is inspected, and the tube is filled with ultrapure water without bubbles. And placing the micro quantitative tube on a mark point of an analytical balance, and reading and recording the mass of the micro quantitative tube and the ultrapure water. Then the device is taken out from the analytical balance, the constant pressure hole on the liquid taking device wall is blocked by a finger, the device is vertically placed above the waste liquid cup, the piston of the liquid taking device is pushed, and the liquid in the trace quantitative tube is emptied.
And 7: the operation in step 6 was repeated 6 times, and the trace quantitative tube plus ultrapure water was recorded as shown in Table 1.
And 8: one end of the micro quantitative tube is inserted into the head end of the latex of the liquid taking device and penetrates through the latex head by about 3 mm. Vertically inserting the sample into a constant-temperature standard substance, plugging a constant-pressure hole on the wall of the liquid taking device by a finger, pulling a piston of the liquid taking device, and extracting a certain serum sample. Repeatedly pushing and pulling the piston of the liquid taking device for 3 times to fully rinse the micro quantitative tube. The spent serum is then emptied.
And step 9: the operation in step 8 was repeated 3 times to sufficiently rinse the micro quantitative tubes.
Step 10: one end of the micro quantitative tube is inserted into the head end of the latex of the liquid taking device and penetrates through the latex head by about 3 mm. The device is vertically inserted into a constant-temperature standard substance, a constant-pressure hole on the wall of the liquid taking device is blocked by a finger, a piston of the liquid taking device is pulled, serum is sucked into the micro quantitative tube, and the piston is stopped being pulled when liquid beads overflow from the upper end. The device is gently taken out of the serum of the standard substance, the constant pressure hole is gently opened after the device is horizontally placed and balanced, and the liquid taking device is slowly rotated to be separated from the micro quantitative tube. And wiping residual liquid on the outer wall of the micro quantitative tube by using dust-free paper. The microtube was examined, and the tube was filled with serum as a standard substance without air bubbles. And placing the micro quantitative tube on a mark point of an analytical balance, and reading and recording the mass of the micro quantitative tube added with the standard substance serum. Then the device is taken out from the analytical balance, the constant pressure hole on the liquid taking device wall is blocked by a finger, the device is vertically placed above the waste liquid cup, the piston of the liquid taking device is pushed, and the liquid in the trace quantitative tube is emptied.
The operation in step 10 was repeated 6 times, and the trace quantitative tube plus ultrapure water was recorded as shown in Table 1.
When the water density at 20 ℃ is known to be 0.998203g/mL, the average value of water contained in the micro quantitative tube 6 times is 0.10003g according to the steps 5 to 7, and the volume V of the micro quantitative tube is 0.100213mL according to the density conversion formula D-m/V & V-m/D.
The mass of the micro quantitative tube serum was calculated according to steps 10 and 11, and the density of the serum was converted using a density conversion formula D of m/V. The single deviation of the serum density measured for 6 times is less than 0.002g/cm3And the mean deviation of 6 measurements is less than 0.001g/cm3。
TABLE 1 data for measuring the density of calcium standards in GBW (E)090623 frozen human serum
The present invention has been described in detail, and the principle and embodiments of the present invention are explained herein by using specific examples, which are only used to help understand the method and the core idea of the present invention. To sum up, the present disclosure should not be construed as limiting the invention, which will be described in the following description but will be modified within the scope of the invention by the spirit of the present disclosure.
Claims (4)
1. A method of measuring density of a liquid using a quantitative liquid take-off device, wherein the quantitative liquid take-off device comprises:
a micro-quantitative container including a body forming a certain volume and openings at both ends of the body, capable of containing a certain volume of liquid and holding the liquid therein to weigh the entire mass, the openings at both ends of the body being formed by first capillaries;
the liquid taking device comprises a cylindrical component, a piston component which can slide in the cylindrical component, and a flexible tubular component which is arranged at a liquid inlet/outlet of the cylindrical component and is communicated with the fluid in the cylindrical component; the flexible tubular member is connectable to and in fluid communication with the micro dosing reservoir; the side wall of the cylindrical part is provided with a constant pressure hole which can communicate the fluid inside the cylindrical part with the outside;
the micro quantitative container can be detachably connected with the liquid taking device and forms fluid communication, so that a certain volume of liquid is extracted or discharged by the micro quantitative container;
the body comprises a body capillary having an inner diameter greater than an inner diameter of the first capillary;
the inner diameter of the main capillary tube is 0.5-6.0 mm, and the length of the main capillary tube is 10-100 mm;
the inner diameter of the first capillary is less than 0.5mm, and the length of the first capillary is 1-20 mm;
the method for measuring the density of the liquid comprises the following steps:
step S1, controlling the temperature of an operation room at a preset temperature, providing a reference liquid and a liquid to be detected, wherein the reference liquid is provided in a sufficient amount, the amount of the liquid to be detected is less than 2mL, and the reference liquid and the liquid to be detected are placed together in the operation room for a specified time;
s2, shaking up the liquid to be measured and dispersing the bubbles in the liquid;
step S3, providing a quality measuring device and a micro quantitative container;
step S4, measuring the mass m of the micro quantitative container by using the mass measuring device0；
Step S5, repeating the operation in step S4 a plurality of times;
step S6, inserting one end of the micro quantitative container into the end of the flexible tubular component of the liquid extractor, and penetrating the flexible tubular component for about 3 mm; vertically inserting the device into constant-temperature reference liquid, blocking a constant-pressure hole on the wall of the liquid taking device, pulling a piston component of the liquid taking device, sucking the reference liquid into a micro quantitative tube, and stopping pulling the piston when liquid beads overflow from the upper end; slightly taking the device out of the reference liquid, horizontally placing and balancing, slightly releasing the constant-pressure hole, and slowly rotating the liquid taking device to separate the liquid taking device from the trace quantitative container; wiping residual liquid on the outer wall of the micro quantitative container by using dust-free paper; checking that the micro quantitative container is filled with reference liquid without bubbles; placing the micro quantitative container on a marking point of the mass measuring device, wherein the mass m1 of the micro quantitative container after the micro quantitative container is filled with the reference liquid; then taking the device out of the quality measuring device, blocking a constant pressure hole on the wall of the liquid taking device, vertically placing the device above the waste liquid cup, pushing a piston of the liquid taking device, and emptying the liquid in the trace quantitative tube;
step S7, repeating the operation in step S6 a plurality of times;
step S8, filling a certain volume of liquid to be measured into the micro quantitative container, fully rinsing the micro quantitative tube, and then emptying;
step S9, repeating the operation of step S8 for a plurality of times, and fully rinsing the micro quantitative container;
step S10, filling the liquid to be measured into the micro quantitative container, and measuring the mass m of the micro quantitative container filled with the liquid to be measured by using the mass measuring device2(ii) a Then emptying the liquid to be detected in the micro quantitative container;
step S11, repeating the operation in step S10 a plurality of times;
step S12, according to the reference liquid density D at the preset temperature0And the mass average value of the micro quantitative container calculated in the steps S5 to S7
Average mass value of reference liquid filled in micro quantitative container
Calculating the volume of the micro quantitative container
Step 13, according to the average mass value of the micro quantitative container filled with the liquid to be measured in the steps S10 and S11
Calculating the density of the liquid to be measured
2. The method for measuring the density of a liquid according to claim 1, wherein the micro quantitative container is a micro quantitative tube.
3. The method for measuring the density of a liquid according to claim 2, wherein the micro quantitative tube is made of a hydrophobic transparent material.
4. The method of measuring a density of a liquid according to claim 1, wherein the cylindrical member has an inner diameter of less than 10mm and a length of less than 85 mm.
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| CN204522997U (en) * | 2014-12-23 | 2015-08-05 | 马荣华 | Quantitative pipette |
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| CN103698248A (en) * | 2013-12-12 | 2014-04-02 | 中国航空工业集团公司北京航空材料研究院 | Method for measuring high-temperature density of lubricating oil |
| CN107271324A (en) * | 2017-07-28 | 2017-10-20 | 国网山东省电力公司电力科学研究院 | A kind of micro volatile liquid density measuring equipment and method |
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