CN109030666A - Identify the method for honeysuckle and Honeysuckle flower using high performance liquid chromatography - Google Patents
Identify the method for honeysuckle and Honeysuckle flower using high performance liquid chromatography Download PDFInfo
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- CN109030666A CN109030666A CN201811173053.8A CN201811173053A CN109030666A CN 109030666 A CN109030666 A CN 109030666A CN 201811173053 A CN201811173053 A CN 201811173053A CN 109030666 A CN109030666 A CN 109030666A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Abstract
The invention discloses a kind of methods for identifying honeysuckle and Honeysuckle flower using high performance liquid chromatography, it is characterized in that, when determinand is one or both of honeysuckle or Honeysuckle flower, the weight percent of vomiting nut acid is interrupted by high performance liquid chromatography detection determinand dry product to identify honeysuckle and Honeysuckle flower, vomiting nut acid content is interrupted less than 1.5% when surveying determinand dry product, is determined as non-honeysuckle；Disconnected vomiting nut acid content is greater than 1.0%, is determined as non-Honeysuckle flower.Disconnected vomiting nut acid content is less than or equal to 1.5%, and when being more than or equal to 1.0%, is determined as the mixture of honeysuckle or Honeysuckle flower.The present invention by high performance liquid chromatography detection honeysuckle and Honeysuckle flower dry product interrupts the weight percent of vomiting nut acid to identify honeysuckle and Honeysuckle flower, quick, accurate, be drug production, circulation, use process in provide and a kind of identify true and false foundation.
Description
Technical field
The invention belongs to Chinese medicine source cultivar identification technical fields, are related to a kind of side for identifying honeysuckle and Honeysuckle flower
Method, and in particular to a method of identify honeysuckle and Honeysuckle flower using high performance liquid chromatography.
Background technique
Honeysuckle and Honeysuckle flower are divided to from 2005 editions Pharmacopoeias of the People's Republic of China is classified as two kinds of medicinal materials.Due to the two original
Plant species are close, medicinal material mode of appearance is similar, it is difficult to distinguish, therefore in the market honeysuckle and Honeysuckle flower crude drug source it is unclear,
Kind is chaotic, because Honeysuckle flower yield is big, price is low, is frequently present of Honeysuckle flower in the market and pretends to be honeysuckle phenomenon, however the two
Material base difference it is larger, to directly influence the curative effect of medicinal material and its preparation, more seriously saponin(e contains in Honeysuckle flower
Amount is more than honeysuckle, and saponin(e can lead to the haemolysis of injection, directly endanger the life security of drug user.
Honeysuckle and Honeysuckle flower can be separated with other medicinal materials with conventional method, but it is existing for honeysuckle and mountain silver
In place of the variety discriminating method of flower between the two comes with some shortcomings.As traditional mode of appearance identifies dependence experience, subjectivity
Property it is big, especially for both in the market melange or crush state and can not identify；DNA molecular identifies law technology complexity, environment
It is tested for ten thousand grades, it is desirable that high, at high cost, round of visits is long, and influence factor leads to poor repeatability more.Pharmacology method verifying it is at high cost,
Period length, influence factor cause error big more, although these methods can to a certain extent distinguish Closely related variety,
The superiority and inferiority of drug effect can not be evaluated.
103173532 B of patent CN (a kind of method and its application for identifying honeysuckle and Honeysuckle flower) is related to a kind of identification
The method and its application of honeysuckle and Honeysuckle flower.This method includes PCR amplification ITS2 nucleotide sequence, including 1) extracts sample
DNA；2) PCR amplification contains the segment of the ITS2 sequence of rDNA；3) amplified production is spliced, obtains complete ITS2
Intergenic region；4) the NJ tree of the ITS2 sequence of sample is constructed.(a kind of quickly identification honeysuckle is true by 104419754 A of patent CN
Pseudo- method) a kind of method of identification honeysuckle and its common adulterant Honeysuckle flower is disclosed, it is to utilize high-resolution solubility curve
Technology analyzes the significant nucleic acid sequences of honeysuckle and Honeysuckle flower.This method to for ensaying honeysuckle flower sample by carrying out
DNA is extracted, PCR amplification and HRM are analyzed, acknowledgement indicator nucleic acid sequences parting, and then is identified the honeysuckle true and false.It is above-mentioned
Method belongs to DNA molecular identification method, needs PCR amplification, and technology is complicated, at high cost.
105277721 B of patent CN (a method of identify the clear preparation raw material Honeysuckle flower of stomatitis and honeysuckle) it discloses
A kind of discrimination method for distinguishing honeysuckle and Honeysuckle flower, comprising the following steps: the preparation with Honeysuckle flower or the raw material containing Honeysuckle flower is
Control；Construct Acute oral inflammatory model；By comparing test sample and reference standards in Acute oral inflammatory model cell
The influence of inflammatory factor TNF-α, IL-6, IL-8 and IL-10 expression identifies and distinguishes honeysuckle and Honeysuckle flower or its compound system
Agent.This method needs the preparation of Honeysuckle flower or the raw material containing Honeysuckle flower for control, constructs Acute oral inflammatory model；There are inspection parties
The defect that method is complicated, inspection is not quick enough, at high cost, influence factor is more, error is big.
Summary of the invention
It is a kind of golden using high performance liquid chromatography identification the purpose of the present invention is to overcome above-mentioned the deficiencies in the prior art, providing
The method of honeysuckle flower and Honeysuckle flower.This method carries out detection honeysuckle and mountain using disconnected vomiting nut acid as index, using liquid chromatography
Honeysuckle flower dry product interrupts the weight percent of vomiting nut acid, to identify honeysuckle and Honeysuckle flower.
To achieve the above object, the present invention adopts the following technical solutions:
The method for identifying honeysuckle and Honeysuckle flower using high performance liquid chromatography, when determinand is in honeysuckle or Honeysuckle flower
When one or two kinds of, the weight percent of vomiting nut acid is interrupted by high performance liquid chromatography detection honeysuckle and Honeysuckle flower dry product
To identify honeysuckle and Honeysuckle flower.
Preferably, in above-mentioned discrimination method, when disconnected vomiting nut acid content is less than 1.5%, it is determined as non-honeysuckle；Disconnected horse
Money acid content is greater than 1.0%, is determined as non-Honeysuckle flower；When disconnected vomiting nut acid content is less than or equal to 1.5%, and it is more than or equal to
When 1.0%, it is determined as the mixture of honeysuckle or Honeysuckle flower.
Preferably, above-mentioned identification honeysuckle and Honeysuckle flower interrupt the detection method of the content of vomiting nut acid are as follows: take reference substance
Solution and test solution inject high performance liquid chromatograph, and peak area is measured at 200-280nm wavelength, using external standard method or
Calibration curve method calculates, and obtains honeysuckle and Honeysuckle flower interrupts the content of vomiting nut acid.
In the above method, taking reference substance solution and test solution to be considered as sample volume is 2-25 μ L.
One step is preferred, and taking reference substance solution and test solution to be considered as sample volume is 2-20 μ L.
It is further preferred that the wave-length coverage of above-mentioned detection method is 230~254nm.
It is further preferred that the most optimum wavelengths of above-mentioned detection method are 240nm.
Preferably, the determination condition of above-mentioned high performance liquid chromatography are as follows:
Chromatographic column C18,4.6mm × 250mm, 5 μm；200~280nm of wavelength；Using acetonitrile as mobile phase A, with pH value for 1.5
~4 aqueous acid is Mobile phase B, using gradient elution, wherein 0~20min of mobile phase A, and percent by volume 5% → 15%；
20~25min, percent by volume 15% → 23%；Remaining is Mobile phase B.
It is further preferred that the aqueous acid includes one or more of phosphoric acid, formic acid, acetic acid, phosphate.
It is further preferred that the preparation step of reference substance solution: taking disconnected vomiting nut acid reference substance, accurately weighed, solubilizer
The solution of 0.05-0.5mg/mL is made.
The preparation of test solution: taking test sample powder 0.25g, accurately weighed, sets in stuffed conical flask, and precision is added molten
Agent 10-50mL, be ultrasonically treated 15-60 minute, shake up, filter to get.
It is further preferred that the power of the ultrasound is 250W, frequency 35kHz.
It is further preferred that the solvent is one of water, 0-50% ethanol solution or 0-50% methanol solution or several
Kind.
Beneficial effects of the present invention:
The present invention interrupts the weight percent of vomiting nut acid by high performance liquid chromatography detection honeysuckle and Honeysuckle flower dry product
It is quick, accurate than identifying honeysuckle and Honeysuckle flower, for provided in the production, circulation, use process of drug it is a kind of identify it is true
False foundation.
Detailed description of the invention
Fig. 1 is disconnected vomiting nut acid reference substance spectrogram；
Fig. 2 is honeysuckle spectrogram；
Fig. 3 is Honeysuckle flower spectrogram；
Fig. 4 is different sources Honeysuckle flower spectrogram；
Fig. 5 is the Honeysuckle flower spectrogram of different sources, different solvents, different drying modes；
Fig. 6 is Honeysuckle flower spectrogram under 254nm Detection wavelength；
Fig. 7 is Henan different batches honeysuckle spectrogram；
Fig. 8 is Hebei different batches honeysuckle spectrogram；
Fig. 9 is Shandong different batches honeysuckle spectrogram.
Specific embodiment
The present invention will be further elaborated with reference to the accompanying drawings and examples, it should which explanation, following the description is only
It is not to be defined to its content to explain the present invention.
Embodiment 1 establishes detection method
1. instrument and material
1.1 instrument
1260 binary high performance liquid chromatograph of Agilent, Agilent G4212B diode array detector, Agilent
Eclipse XDB-C18Chromatographic column (150mm × 4.6mm, 5 μm), KH500B type ultrasonic cleaning machine (Kunshan GRAD), bright and limpid D24UV
Ultrapure water system (Mi Libo China).
1.2 materials and reagent
Acetonitrile (FisherScientific) is chromatographic grade, and water is level-one ultrapure water, other examinations such as dehydrated alcohol (COMIO)
Agent is analysis level；Disconnected vomiting nut acid reference substance is purchased to be produced in Shanghai Shi Dande standard technique Services Co., Ltd, lot number:
60077-46-5。
2. test method
2.1 chromatographic condition
Chromatographic column C18,4.6mm × 250mm, 5 μm；Wavelength 240nm；Using acetonitrile as mobile phase A, the phosphorus for being 2.0 with pH value
Aqueous acid is Mobile phase B, using gradient elution, wherein 0~20min of mobile phase A, and percent by volume 5% → 15%；20~
25min, percent by volume 15% → 23%；Remaining is Mobile phase B.
The preparation of 2.2 reference substance solutions
Disconnected vomiting nut acid reference substance is taken, it is accurately weighed, add 10% ethyl alcohol that the solution of 0.27mg/mL is made.
The preparation of 2.3 test solutions
Sample powder (crossing No. four sieves) about 0.25g is taken, it is accurately weighed, it sets in stuffed conical flask, 10% ethyl alcohol is added in precision
25ml, ultrasonic treatment (power 250W, frequency 35kHz) 30 minutes, shakes up, filtration to get.
2.4 methodological study
2.4.1 linear to investigate
Precision draws reference substance solution (0.27mg/mL) 2,5,10,15,20,25 μ l, is injected separately into liquid chromatograph, remembers
Record peak area.Linear regression, regression equation are as follows: A=1182*C-24.2, phase are carried out to sample introduction quality (m, μ g) with peak area (A)
Relationship number R=0.9998, the results showed that disconnected vomiting nut acid solution is in good linear relationship between 0.54 μ of μ g~6.75 g.Knot
Fruit is shown in Table 1.
1 linear relationship of table is investigated
2.4.2 instrument precision
Precision draws reference substance solution (0.27mg/mL) 10 μ L, injects liquid chromatograph, records peak area, computing equipment
Precision is as a result as follows:
2 instrument precision of table is investigated
The result shows that: average peak area 3182.2, RSD=0.43%, instrument precision is good.
2.4.3 repeated
Same batch of sample (Shandong pan honeysuckle flower) is taken, takes 6 parts in parallel, it is molten to prepare test sample by above-mentioned content assaying method
Liquid and reference substance solution, accurate each 10 μ L of draw solution inject liquid chromatograph, record peak area, calculate content, as a result table
Bright, the method measures the content of disconnected vomiting nut acid, and repeatability is good.As a result as follows:
3 repeated experiment of table is investigated
The result shows that: RSD=1.83%, repeated experiment are good.
The measurement of 2.5 samples
Reference substance solution and each 10 μ L of test solution are taken, high performance liquid chromatograph is injected, peak face is measured at 240nm
Product calculates the content that vomiting nut acid is interrupted to get honeysuckle and Honeysuckle flower using external standard method.The result is shown in Figure 1-3.
2 confirmatory experiment of embodiment
1. different sources Honeysuckle flower is verified.
Using different sources Honeysuckle flower as research object, content is measured using the testing conditions of method described in embodiment 1,
As a result see Fig. 4.
Spectrogram is followed successively by Chongqing Liberation Road Honeysuckle flower from top to bottom in figure, Shandong Poly provides Guizhou Honeysuckle flower, Shandong is protected
Benefit provides Guizhou and produces Honeysuckle flower, Shandong Poly offer Hunan production Honeysuckle flower.
Map and Fig. 4 are compareed in conjunction with disconnected vomiting nut acid it can be seen that different sources Honeysuckle flower map interruption vomiting nut acid is right
According to product corresponding position without or have micro chromatographic peak, be computed content far below 1.0%.
2. the Honeysuckle flower verifying under the conditions of different sources, different solvents, different drying modes etc..
It using different sources Honeysuckle flower as research object, is extracted using different solvents, according to the detection of 1 the method for embodiment
Condition measures content, and spectrogram is shown in Fig. 5.
Spectrogram shown in Fig. 5 is from top to bottom successively are as follows: red body hairy honeysuckle 1 (solvent is water), reference substance (solvent is water), yellow
(solvent is for brown hair Honeysuckle flower (solvent is 50% methanol), Yiwu Honeysuckle flower (solvent is 50% methanol), Nanning Mashan Honeysuckle flower
50% methanol), the 3rd batch, Guizhou Honeysuckle flower (solvent is 50% methanol), the 2nd batch, Guizhou Honeysuckle flower (solvent is 50% methanol), south
It is peaceful Mashan Honeysuckle flower (solvent is water), the 3rd batch, Guizhou Honeysuckle flower (solvent is water), the 4th batch, Guizhou Honeysuckle flower (solvent is water), expensive
The 5th batch, state Honeysuckle flower (solvent is water), grey felt hair Honeysuckle flower (solvent is water), yellowish-brown hair Honeysuckle flower (solvent is water), Anhui are red
Glandular hairs Honeysuckle flower (solvent is water) is lyophilized that green Honeysuckle flower (solvent is water), to dry brown Honeysuckle flower (solvent is water), Guizhou red
Glandular hairs Honeysuckle flower (solvent is water).
As seen from Figure 5: water, 50% methanol all can serve as the Extraction solvent of disconnected vomiting nut acid；Different sources, difference
The Honeysuckle flower map of solvent extraction interrupt the corresponding position of vomiting nut acid reference substance without or have micro chromatographic peak, be computed disconnected
The content of vomiting nut acid is far below 1.0%.
3. the sample of different wave length is verified
Using different batches ash felt hair Honeysuckle flower as research object, according to the testing conditions of 1 the method for embodiment in 254nm
Place's measurement content, as a result as shown in Figure 6.
Spectrogram is from top to bottom successively in Fig. 6 are as follows: disconnected vomiting nut acid reference substance, the yellowish-brown hair Honeysuckle flower of ash, grey felt hair Honeysuckle flower
1st batch, grey felt hair Honeysuckle flower the 2nd batch, grey felt hair Honeysuckle flower the 3rd batch, grey felt hair Honeysuckle flower the 4th batch, grey felt hair Honeysuckle flower the 5th
It criticizes, grey Honeysuckle flower the 6th batch, felt hair.
As seen from Figure 6: 254nm can be used as the Detection wavelength for detecting disconnected vomiting nut acid；Different batches Honeysuckle flower figure
Spectrum interrupt vomiting nut acid reference substance corresponding position without or have micro chromatographic peak, be computed content far below 1.0%.
4. different batches, different market specifications, the verifying of different sources honeysuckle sample
Using different batches, different market specifications, honeysuckle is research object to different sources (Shandong, Henan), according to implementation
The testing conditions of method described in example 1 measure content, as a result as shown in Fig. 7-9.
Spectrogram is from top to bottom successively in Fig. 7 are as follows: Henan goods of inferior quality the 1st batch, Henan goods of inferior quality the 2nd batch, Henan goods of inferior quality the 3rd
It criticizes, Henan goods of inferior quality the 4th batch, Henan goods of inferior quality the 5th batch.
Spectrogram is from top to bottom successively in Fig. 8 are as follows: Hebei third grade product the 3rd batch, Hebei third grade product the 1st batch, Hebei third grade product the 2nd
Batch.
Spectrogram is from top to bottom successively in Fig. 9 are as follows: Shandong produce the 4th batch, Shandong produce the 1st batch, Shandong produce the 2nd batch, Shandong produce the 3rd
Batch.
Map and Fig. 7-9 are compareed in conjunction with disconnected vomiting nut acid it can be seen that different batches, different market specifications, different sources
Honeysuckle map, which interrupts vomiting nut acid reference substance corresponding position, apparent chromatographic peak, is computed content much higher than 1.5%.
5. the honeysuckle of different sources, different batches interrupts vomiting nut acid content
Take Hebei province, Henan Province and Shandong Province each batch honeysuckle according to the detector bar of method described in embodiment 1 respectively
Part is detected, and gained testing result is as shown in table 4.
4 Hebei province of table, Henan Province and Shandong Province's each batch honeysuckle testing result
6. the Honeysuckle flower of different sources, different batches interrupts vomiting nut acid content
Take Guangdong Province, Guizhou Province and Hunan Province's each batch Honeysuckle flower according to the detector bar of method described in embodiment 1 respectively
Part is detected, and gained testing result is as shown in table 5.
5 Guangdong Province of table, Guizhou Province and Hunan Province's each batch Honeysuckle flower testing result
Pass through the detection of honeysuckle and Honeysuckle flower to different sources, different batches, it was demonstrated that this method can quickly, accurately
Identification honeysuckle and Honeysuckle flower.
Above-mentioned, although the foregoing specific embodiments of the present invention is described with reference to the accompanying drawings, not protects model to the present invention
The limitation enclosed, based on the technical solutions of the present invention, those skilled in the art are not needed to make the creative labor and can be done
Various modifications or changes out are still within protection scope of the present invention.
Claims (10)
1. identifying the method for honeysuckle and Honeysuckle flower using high performance liquid chromatography, which is characterized in that when determinand dry product is gold
When one or both of honeysuckle flower or Honeysuckle flower, the weight of vomiting nut acid is interrupted by high performance liquid chromatography detection determinand dry product
Percentage is measured to identify honeysuckle and Honeysuckle flower.
2. the method according to claim 1 for identifying honeysuckle and Honeysuckle flower, which is characterized in that when in determinand dry product
Vomiting nut acid content of breaking is determined as non-honeysuckle less than 1.5%；Disconnected vomiting nut acid content is greater than 1.0%, is determined as Fei Shanyin
Flower；Disconnected vomiting nut acid content is less than or equal to 1.5%, and when being more than or equal to 1.0%, is determined as the mixing of honeysuckle or Honeysuckle flower
Object.
3. the method according to claim 1 or 2 for identifying honeysuckle and Honeysuckle flower, which is characterized in that the disconnected vomiting nut
The detection method of the content of acid are as follows: take reference substance solution and test solution, high performance liquid chromatograph is injected, in 200-280nm
Peak area is measured at wavelength, is calculated using external standard method or calibration curve method, obtains honeysuckle and Honeysuckle flower interrupts vomiting nut acid
Content.
4. the method according to claim 3 for identifying honeysuckle and Honeysuckle flower, which is characterized in that the high performance liquid chromatography
The determination condition of method are as follows:
Chromatographic column C18,4.6mm × 250mm, 5 μm；200~280nm of wavelength；Using acetonitrile as mobile phase A, with pH value for 1.5~4
Aqueous acid be Mobile phase B, using gradient elution, wherein 0~20min of mobile phase A, percent by volume 5% → 15%；20~
25min, percent by volume 15% → 23%；Remaining is Mobile phase B.
5. the method according to claim 4 for identifying honeysuckle and Honeysuckle flower, which is characterized in that the aqueous acid includes
One of phosphoric acid, formic acid, acetic acid, phosphate.
6. it is according to claim 4 identify honeysuckle and Honeysuckle flower method, which is characterized in that Detection wavelength be 230~
254nm。
7. the method according to claim 3 for identifying honeysuckle and Honeysuckle flower, which is characterized in that the reference substance solution
The preparation method comprises the following steps: taking disconnected vomiting nut acid reference substance, accurately weighed, the solution of 0.05-0.5mg/mL is made in solubilizer.
8. the method according to claim 3 for identifying honeysuckle and Honeysuckle flower, which is characterized in that the test solution
The preparation method comprises the following steps: test sample powder 0.25g is taken, and it is accurately weighed, it sets in stuffed conical flask, solvent 10-50mL, ultrasound is added in precision
Processing 15-60 minute, shake up, filter to get.
9. the method according to claim 8 for identifying honeysuckle and Honeysuckle flower, which is characterized in that the power of the ultrasound is
250W, frequency 35kHz.
10. the method according to claim 8 for identifying honeysuckle and Honeysuckle flower, which is characterized in that the solvent is water, 0-
One or more of 50% ethanol solution or 0-50% methanol solution.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111198236A (en) * | 2020-01-14 | 2020-05-26 | 山东中医药大学 | Method for identifying honeysuckle and lonicera confusa by utilizing high performance liquid chromatography and liquid chromatography-mass spectrometry |
| CN112816433A (en) * | 2020-12-31 | 2021-05-18 | 中国医学科学院药用植物研究所 | Method, equipment and medium for identifying lonicera confusa based on infrared spectrum |
| CN113740474A (en) * | 2021-08-27 | 2021-12-03 | 深圳市药品检验研究院（深圳市医疗器械检测中心） | Honeysuckle and lonicera confusa identification model combining anti-new coronavirus effect and construction method and application thereof |
| CN114689753A (en) * | 2022-03-31 | 2022-07-01 | 广东一方制药有限公司 | Method for identifying honeysuckle in different Yuanyuan mountain |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100359251B1 (en) * | 1994-12-28 | 2003-01-08 | 주식회사 엘지생명과학 | Method for extracting 5-hydroxymethyl-2-furfural from lonicera japonica thunb |
| CN101628023A (en) * | 2009-02-24 | 2010-01-20 | 广州白云山和记黄埔中药有限公司 | Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof |
| CN103808751A (en) * | 2013-01-30 | 2014-05-21 | 河南省科高植物天然产物开发工程技术有限公司 | Method for identifying lonicera japonica medicinal material or derivatives of lonicera japonica medicinal material |
| CN105606734A (en) * | 2016-01-05 | 2016-05-25 | 华中科技大学 | Method for detecting honeysuckle flower and lonicerae flos medicinal materials through rapid resolution liquid chromatography |
| CN106226415A (en) * | 2016-07-11 | 2016-12-14 | 山东省分析测试中心 | A kind of method of six kinds of iridoid glycoside constituents in Flos Lonicerae of mensuration simultaneously |
| CN106248817A (en) * | 2016-07-11 | 2016-12-21 | 山东省分析测试中心 | A kind of quality of Flos Lonicerae evaluation methodology |
| CN108469473A (en) * | 2018-02-09 | 2018-08-31 | 山东省分析测试中心 | A method of utilizing metabolite of the RRLC-DAD-ESI-Q-TOF-MS detection honeysuckles in rat blood serum |
-
2018
- 2018-10-09 CN CN201811173053.8A patent/CN109030666B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100359251B1 (en) * | 1994-12-28 | 2003-01-08 | 주식회사 엘지생명과학 | Method for extracting 5-hydroxymethyl-2-furfural from lonicera japonica thunb |
| CN101628023A (en) * | 2009-02-24 | 2010-01-20 | 广州白云山和记黄埔中药有限公司 | Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof |
| CN103808751A (en) * | 2013-01-30 | 2014-05-21 | 河南省科高植物天然产物开发工程技术有限公司 | Method for identifying lonicera japonica medicinal material or derivatives of lonicera japonica medicinal material |
| CN105606734A (en) * | 2016-01-05 | 2016-05-25 | 华中科技大学 | Method for detecting honeysuckle flower and lonicerae flos medicinal materials through rapid resolution liquid chromatography |
| CN106226415A (en) * | 2016-07-11 | 2016-12-14 | 山东省分析测试中心 | A kind of method of six kinds of iridoid glycoside constituents in Flos Lonicerae of mensuration simultaneously |
| CN106248817A (en) * | 2016-07-11 | 2016-12-21 | 山东省分析测试中心 | A kind of quality of Flos Lonicerae evaluation methodology |
| CN108469473A (en) * | 2018-02-09 | 2018-08-31 | 山东省分析测试中心 | A method of utilizing metabolite of the RRLC-DAD-ESI-Q-TOF-MS detection honeysuckles in rat blood serum |
Non-Patent Citations (6)
| Title |
|---|
| AI-LI GUO 等: "Influence of Sulfur Fumigation on the Chemical Constituents and Antioxidant Activity of Buds of Lonicera japonica", 《MOLECULES》 * |
| LIAN‐WEN QI 等: "Structural characterization and identification of iridoid glycosides, saponins, phenolic acids and flavonoids in Flos Lonicerae Japonicae by a fast liquid chromatography method with diode-array detection and time-of-flight mass spectrometry", 《RAPID COMMUNICATIONS IN MASS SPECTROMETRY》 * |
| ZONG‐YUN LI 等: "Secoiridoid Sulfonates from the Sulfiting-Processed Buds of Lonicera japonica", 《HELVETICA CHIMICA ACTA》 * |
| 朱姮 等: "HPLC-DAD-ESI-Q-TOF/MS法测定金银忍冬花中的化学成分", 《中草药》 * |
| 李泮霖 等: "基于UFLC-Triple-Q-TOF-MS/MS技术的金银花、山银花化学成分比较", 《中南药学》 * |
| 郭爱丽 等: "硫磺熏蒸金银花中断马钱子酸亚硫酸衍生物产生的机制探讨", 《中国中药杂志》 * |
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| CN111198236A (en) * | 2020-01-14 | 2020-05-26 | 山东中医药大学 | Method for identifying honeysuckle and lonicera confusa by utilizing high performance liquid chromatography and liquid chromatography-mass spectrometry |
| CN112816433A (en) * | 2020-12-31 | 2021-05-18 | 中国医学科学院药用植物研究所 | Method, equipment and medium for identifying lonicera confusa based on infrared spectrum |
| CN112816433B (en) * | 2020-12-31 | 2023-09-22 | 中国医学科学院药用植物研究所 | Method, equipment and medium for identifying lonicera japonica based on infrared spectrum |
| CN113740474A (en) * | 2021-08-27 | 2021-12-03 | 深圳市药品检验研究院（深圳市医疗器械检测中心） | Honeysuckle and lonicera confusa identification model combining anti-new coronavirus effect and construction method and application thereof |
| WO2023024288A1 (en) * | 2021-08-27 | 2023-03-02 | 深圳市药品检验研究院（深圳市医疗器械检测中心） | Lonicera japonica thunb. and lonicera macranthoides hand.-mazz. identification model combined with anti sars-cov-2mpro effect, and construction method therefor and application thereof |
| CN114689753A (en) * | 2022-03-31 | 2022-07-01 | 广东一方制药有限公司 | Method for identifying honeysuckle in different Yuanyuan mountain |
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