CN109030602A - A kind of electric potential type organophosphorus biosensor and preparation method thereof - Google Patents
A kind of electric potential type organophosphorus biosensor and preparation method thereof Download PDFInfo
- Publication number
- CN109030602A CN109030602A CN201810906777.2A CN201810906777A CN109030602A CN 109030602 A CN109030602 A CN 109030602A CN 201810906777 A CN201810906777 A CN 201810906777A CN 109030602 A CN109030602 A CN 109030602A
- Authority
- CN
- China
- Prior art keywords
- preparation
- biosensor
- electric potential
- organophosphorus
- potential type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 239000012528 membrane Substances 0.000 claims abstract description 29
- 102000004190 Enzymes Human genes 0.000 claims abstract description 25
- 108090000790 Enzymes Proteins 0.000 claims abstract description 25
- 229940088598 enzyme Drugs 0.000 claims abstract description 25
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 claims abstract description 13
- 235000001206 Amorphophallus rivieri Nutrition 0.000 claims abstract description 13
- 229920002581 Glucomannan Polymers 0.000 claims abstract description 13
- 229920002752 Konjac Polymers 0.000 claims abstract description 13
- 229940046240 glucomannan Drugs 0.000 claims abstract description 13
- 239000000252 konjac Substances 0.000 claims abstract description 13
- 235000010485 konjac Nutrition 0.000 claims abstract description 13
- 102000012440 Acetylcholinesterase Human genes 0.000 claims abstract description 11
- 108010022752 Acetylcholinesterase Proteins 0.000 claims abstract description 11
- 229940022698 acetylcholinesterase Drugs 0.000 claims abstract description 11
- -1 polyethylene terephthalate Polymers 0.000 claims abstract description 11
- 229920000139 polyethylene terephthalate Polymers 0.000 claims abstract description 7
- 239000005020 polyethylene terephthalate Substances 0.000 claims abstract description 7
- 238000004108 freeze drying Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000008055 phosphate buffer solution Substances 0.000 claims description 3
- 229910052698 phosphorus Inorganic materials 0.000 claims description 3
- 239000011574 phosphorus Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 2
- 244000247812 Amorphophallus rivieri Species 0.000 claims 3
- 235000006481 Colocasia esculenta Nutrition 0.000 claims 1
- 240000004270 Colocasia esculenta var. antiquorum Species 0.000 claims 1
- 230000006196 deacetylation Effects 0.000 claims 1
- 238000003381 deacetylation reaction Methods 0.000 claims 1
- 239000003292 glue Substances 0.000 claims 1
- 239000003987 organophosphate pesticide Substances 0.000 abstract description 16
- 241001312219 Amorphophallus konjac Species 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 7
- 238000001514 detection method Methods 0.000 abstract description 5
- 239000011521 glass Substances 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 239000000575 pesticide Substances 0.000 description 11
- 239000000243 solution Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 7
- KKEYFWRCBNTPAC-UHFFFAOYSA-N Terephthalic acid Chemical compound OC(=O)C1=CC=C(C(O)=O)C=C1 KKEYFWRCBNTPAC-UHFFFAOYSA-N 0.000 description 6
- JUGOREOARAHOCO-UHFFFAOYSA-M acetylcholine chloride Chemical compound [Cl-].CC(=O)OCC[N+](C)(C)C JUGOREOARAHOCO-UHFFFAOYSA-M 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 5
- OEBRKCOSUFCWJD-UHFFFAOYSA-N dichlorvos Chemical compound COP(=O)(OC)OC=C(Cl)Cl OEBRKCOSUFCWJD-UHFFFAOYSA-N 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 5
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000001548 drop coating Methods 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N ethylene glycol Natural products OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of electric potential type organophosphorus biosensors and preparation method thereof.The biosensor is used for the quick detection of organophosphorus pesticide.Acetylcholinesterase is fixed on the organic nucleopore membranes basis material of polyethylene terephthalate by the present invention using Deacetyled konjac glucomannan colloidal sol investment; the enzyme membrane of preparation is fixed in the glass electrode of pH meter with ' O ' shape circle again, obtains organophosphorus pesticide biosensor.The organophosphorus pesticide sensor of this method preparation has the characteristics that high sensitivity, response are fast, at low cost.
Description
Technical field
The present invention relates to a kind of electric potential type organophosphorus biosensors and preparation method thereof, belong to analysis and testing technology neck
Domain.
Background technique
The event that pesticide residue causes people, animal to be poisoned happens occasionally, and the hidden danger such as carcinogenic, teratogenesis and cause gene mutation more enable
People's worry.Organophosphorus pesticide is that use scope is most wide, a kind of maximum pesticide of usage amount.Current organophosphorus pesticide detection side
Method be mainly chromatography [Wang Zongxian, Gao Zhixian, Ma Cheng, the progress of organic phosphorus detection method, China Health, 2003,13 (4),
401-403].It is high to the technical requirements of testing staff since chromatography pretreatment process is cumbersome, be not suitable for field quick detection.
Biosensor technology based on electrochemical analysis principle has quick, sensitive, accurate and is easy to continuous and automatic
The characteristics of, it can satisfy the needs of field quick detection.In existing electric potential type organophosphorus pesticide biosensor, it is common that
Acetylcholinesterase is fixed on nylon wire using cross-linking method or covalent bonding method, is then fixed nylon wire with ' O ' shape circle
On to the glass electrode of pH meter [immobilization research of the acetylcholinesterase on nylon wire, chemistry world, 2007,48 (12):
751-753；The research of acetylcholinesterasebiosensor biosensor, pesticide, 2008,47 (8): 577-579].The enzyme membrane being prepared
Unevenly, and the compactness of enzyme membrane and electrode is not high, thus the sensor consistency and repeatability prepared are not ideal enough.
Summary of the invention
For overcome the deficiencies in the prior art, the purpose of the present invention is to provide a kind of electric potential type organophosphorus biosensors
And preparation method thereof.Acetylcholinesterase is fixed in the organic nucleopore membranes of polyethylene terephthalate by the present invention, preparation
Sensor structure out is stablized, consistency and reproducible.
Acetylcholinesterase is fixed on poly- to benzene two by the present invention using Deacetyled konjac glucomannan colloidal sol investment
On the organic nucleopore membranes basis material of formic acid glycol ester, then the enzyme membrane of preparation is fixed on to the glass electrode of pH meter with ' O ' shape circle
On, obtain electric potential type organophosphorus biosensor.Technical solution of the present invention is specifically described as follows.
The present invention provides a kind of preparation method of electric potential type organophosphorus biosensor, comprising the following steps:
(1) preparation of Deacetyled konjac glucomannan colloidal sol
It is 0.8~1.2g/L's by 0.8~1.2g Deacetyled konjac glucomannan fine powder and 80~120ml concentration
Na2CO3After solution is mixed into translucent colloidal liquid, it is further continued for 15~25min of stirring, it is sweet that deacetylated konjac Portugal is made
Glycan colloidal sol；
(2) preparation of enzyme membrane
The acetylcholinesterase, 0.8~1.2ml phosphate buffer solution and 0.8~1.2ml for weighing 0.8~1.2mg are deacetylated
Base KGM Sol is uniformly mixed, obtains mixed solution；Take 2~5 μ L mixing drop-coated in poly terephthalic acid second two
In the organic nucleopore membranes of alcohol ester, then it is placed in refrigerator freeze-drying lyophilization at a temperature of 4 DEG C, obtains enzyme membrane；
(3) preparation of enzyme electrode
Enzyme membrane is fixed on the combination electrode of pH meter with ' O ' shape circle, enzyme electrode is made, is i.e. the organic phosphorus biology of electric potential type passes
Sensor.
In the present invention, in step (2), the aperture of the organic nucleopore membranes of polyethylene terephthalate is 1-10 μm.
The present invention also provides electric potential type organophosphorus biosensors made from a kind of above-mentioned preparation method.
Compared to the prior art, the beneficial effects of the present invention are:
Acetylcholinesterase is fixed on poly terephthalic acid using Deacetyled konjac glucomannan investment by the present invention
In the organic nucleopore membranes of glycol ester, enzyme membrane is obtained with good hydrophilic property, good penetrability, flat and smooth, high mechanical strength, flexibility
The advantages that good.And then the Copper diethlydithiocarbamate prepared using the enzyme membrane, the transducer sensitivity is high, response is fast, consistency and
Reproducible advantage.
Detailed description of the invention
Fig. 1 is pesticide to the inhibiting rate of the sensor potential response value in embodiment 1 and the logarithm relationship of pesticide concentration.
Specific embodiment
The present invention is described in further detail below by embodiment, but is not intended to limit the present invention.
Embodiment 1
1. the preparation of Deacetyled konjac glucomannan colloidal sol
It weighs 0.8g Deacetyled konjac glucomannan fine powder and is added to the Na that 100ml concentration is 1.0g/L2CO3In solution,
It is stirred simultaneously with electric blender.Become translucent colloidal liquid in solution, stirs 20min with electric blender again at this time.It will
The colloidal sol of preparation is transferred in bottle,suction, is filtered bottleneck and is blocked with rubber stopper, is evacuated to bubble-free in colloidal sol.
2. the preparation of enzyme membrane
The acetylcholinesterase of 1mg is weighed in centrifuge tube, and 1ml phosphate buffer solution is added, it is deacetylated to add 1ml
Base KGM Sol is uniformly mixed.Take the poly terephthalic acid second two that the above-mentioned mixing drop-coated of 2 μ l is 10 μm in aperture
In the organic nucleopore membranes of alcohol ester, it is placed in 4 DEG C of freeze-drying lyophilizations in refrigerator.
3. the preparation of enzyme electrode
The enzyme membrane prepared is fixed on the combination electrode of pH meter with ' O ' shape circle, obtains enzyme electrode.
Embodiment 2
With embodiment 1, difference is only that the organic nucleopore membranes of polyethylene terephthalate for other implementation conditions and parameter
Aperture be 5 μm.
Embodiment 3
With embodiment 1, difference is only that it is the organic nucleopore of polyethylene terephthalate for other implementation conditions and parameter
The aperture of film is 1 μm.
Embodiment 4
With embodiment 1, difference is only that the Deacetyled konjac glucomannan colloidal sol of drop coating for other implementation conditions and parameter
Volume with the mixed liquor of acetylcholinesterase is 3 μ l.
Embodiment 5
With embodiment 1, difference is only that the Deacetyled konjac glucomannan colloidal sol of drop coating for other implementation conditions and parameter
Volume with the mixed liquor of acetylcholinesterase is 5 μ l.
Application Example 1
By organophosphorus pesticide biosensor assay organophosphorus pesticide DDVP made from embodiment 1.Temperature is 30 DEG C, bottom
Object (acecoline solution) concentration is 8 × 10-3mol·L-1, in pH=7.0 buffer solution, incubation of the enzyme membrane in pesticide
Time is 3min.
Pesticide is as shown in Figure 1 to the inhibiting rate of sensor potential response value and the logarithm relationship of pesticide concentration.As a result table
Bright, linear response range is 1~10mg/L, and Monitoring lower-cut is up to 0.4mg/L.
The test-strips for extracting 10 batches test same actual sample, test relative standard deviation less than 3%.Test
For item to substrate METHOD FOR CONTINUOUS DETERMINATION 10 times, test relative deviation is 2.8%.
Above-mentioned test result shows sensor sensitivity with higher, faster response time and good consistency
And repeatability.
Application Example 2
By organophosphorus pesticide biosensor assay organophosphorus pesticide DDVP made from embodiment 2.Temperature is 30 DEG C, bottom
Object (acecoline solution) concentration is 8 × 10-3mol·L-1, in pH=7.0 buffer solution, incubation of the enzyme membrane in pesticide
Time is 4min.Linear sensor response range is 1~10mg/L, and Monitoring lower-cut is up to 0.4mg/L.
Application Example 3
By organophosphorus pesticide biosensor assay organophosphorus pesticide DDVP made from embodiment 3.Temperature is 30 DEG C, bottom
Object (acecoline solution) concentration is 8 × 10-3mol·L-1, in pH=7.0 buffer solution, incubation of the enzyme membrane in pesticide
Time is 6min.Linear sensor response range is 1~10mg/L, and Monitoring lower-cut is up to 0.4mg/L.
Application Example 4
By organophosphorus pesticide biosensor assay organophosphorus pesticide DDVP made from embodiment 4.Temperature is 30 DEG C, bottom
Object (acecoline solution) concentration is 8 × 10-3mol·L-1, in pH=7.0 buffer solution, incubation of the enzyme membrane in pesticide
Time is 3min.Linear sensor response range is 2~8mg/L, and Monitoring lower-cut is up to 0.2mg/L.
Application Example 5
By organophosphorus pesticide biosensor assay organophosphorus pesticide DDVP made from embodiment 5.Temperature is 30 DEG C, bottom
Object (acecoline solution) concentration is 8 × 10-3mol·L-1, in pH=7.0 buffer solution, incubation of the enzyme membrane in pesticide
Time is 3min.Linear sensor response range is 2~6mg/L, and Monitoring lower-cut is up to 0.1mg/L.
Claims (3)
1. a kind of preparation method of electric potential type organophosphorus biosensor, which comprises the following steps:
(1) preparation of Deacetyled konjac glucomannan colloidal sol
The Na for being 0.8~1.2g/L by 0.8~1.2g Deacetyled konjac glucomannan fine powder and 80~120ml concentration2CO3It is molten
After liquid is mixed into translucent colloidal liquid, it is further continued for 15~25min of stirring, it is molten that Deacetyled konjac glucomannan is made
Glue；
(2) preparation of enzyme membrane
Weigh the acetylcholinesterase, 0.8~1.2ml phosphate buffer solution and 0.8~1.2ml deacetylation evil spirit of 0.8~1.2mg
Taro Glucomannan colloidal sol is uniformly mixed, obtains mixed solution；Take 2~5 μ L mixing drop-coated in polyethylene terephthalate
In organic nucleopore membranes, then it is placed in refrigerator freeze-drying lyophilization at a temperature of 4 DEG C, obtains enzyme membrane；
(3) preparation of enzyme electrode
Enzyme membrane is fixed on the combination electrode of pH meter with ' O ' shape circle, enzyme electrode, the i.e. organic phosphorus bio-sensing of electric potential type is made
Device.
2. preparation method according to claim 1, which is characterized in that in step (2), polyethylene terephthalate has
The aperture of machine nucleopore membranes is 1-10 μm.
3. it is a kind of according to claim 1~one of 2 described in electric potential type organophosphorus biosensor made from preparation method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810906777.2A CN109030602B (en) | 2018-08-10 | 2018-08-10 | A kind of electric potential type organophosphorus biosensor and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810906777.2A CN109030602B (en) | 2018-08-10 | 2018-08-10 | A kind of electric potential type organophosphorus biosensor and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109030602A true CN109030602A (en) | 2018-12-18 |
| CN109030602B CN109030602B (en) | 2019-12-03 |
Family
ID=64633419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810906777.2A Expired - Fee Related CN109030602B (en) | 2018-08-10 | 2018-08-10 | A kind of electric potential type organophosphorus biosensor and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109030602B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004046374A1 (en) * | 2002-11-21 | 2004-06-03 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Biosensor and method comprising enzymes immobilized on semiconductors |
| CN101050455A (en) * | 2007-03-29 | 2007-10-10 | 中国农业科学院农产品加工研究所 | Immobilization lipase, and preparation method |
| CN104198559A (en) * | 2014-09-15 | 2014-12-10 | 常熟理工学院 | Electrochemical biosensor for organic phosphorus pesticide detection and preparation method of electrochemical biosensor |
-
2018
- 2018-08-10 CN CN201810906777.2A patent/CN109030602B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004046374A1 (en) * | 2002-11-21 | 2004-06-03 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Biosensor and method comprising enzymes immobilized on semiconductors |
| CN101050455A (en) * | 2007-03-29 | 2007-10-10 | 中国农业科学院农产品加工研究所 | Immobilization lipase, and preparation method |
| CN104198559A (en) * | 2014-09-15 | 2014-12-10 | 常熟理工学院 | Electrochemical biosensor for organic phosphorus pesticide detection and preparation method of electrochemical biosensor |
Non-Patent Citations (3)
| Title |
|---|
| 田园: "快速检测有机磷及氨基甲酸酯类农药的酶生物传感器研究", 《中国优秀硕士学位论文全文数据库 信息科技辑》 * |
| 郭晓明等: "基于脱乙酰基魔芋葡甘聚糖固定酶的H2O2生物传感器", 《分析测试学报》 * |
| 郭晓明等: "基于脱乙酰基魔芋葡甘聚糖固定酶的葡萄糖传感器", 《分析试验室》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109030602B (en) | 2019-12-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102262115B (en) | Electrochemical immunosensor for melamine content determination, and preparation method and application thereof | |
| Chen et al. | A gold nanoparticles/sol–gel composite architecture for encapsulation of immunoconjugate for reagentless electrochemical immunoassay | |
| US5707502A (en) | Sensors for measuring analyte concentrations and methods of making same | |
| CN102759631B (en) | The latex enhancing immune of a kind of quantitative detection Procalcitonin PCT is than turbid kit | |
| Lin et al. | Disposable biosensor based on enzyme immobilized on Au–chitosan-modified indium tin oxide electrode with flow injection amperometric analysis | |
| CN106198659B (en) | A method of depositing nanogold in micro-fluidic duct | |
| CN101620227A (en) | Multi-channel chip for cholera diagnosis based on structural conductive macromolecular material technology | |
| CN111693571B (en) | Method for detecting GPC3 based on light addressing potential sensor | |
| Ionescu et al. | Amperometric immunosensor for the detection of anti-West Nile virus IgG | |
| CN111551612B (en) | Plasticizer-free molecularly imprinted polymer membrane ion selective electrode sensitive membrane | |
| Ikariyama et al. | Micro-enzyme electrode prepared on platinized platinum | |
| CN107966432A (en) | A kind of Soluble growth that measures stimulates the kit and its test method of 2 protein content of expressing gene | |
| Marzouk et al. | Prototype amperometric biosensor for sialic acid determination | |
| CN110441528B (en) | Mo based on core-shell structure2Construction of C @ C nanosphere cardiac troponin I immunosensor | |
| CN104198714B (en) | A kind of electrochemical immunosensor and preparation and application thereof | |
| CN109030602B (en) | A kind of electric potential type organophosphorus biosensor and preparation method thereof | |
| CN114966007A (en) | Microsphere composition for homogeneous phase chemiluminescence detection | |
| Sun et al. | Preparation of hemoglobin imprinted polymers based on graphene and protein removal assisted by electric potential | |
| CN103823071B (en) | " magnetic bead glycolated hemoglobin ferrocene boric acid " complex and application thereof | |
| Li et al. | On-chip determination of glycated hemoglobin with a novel boronic acid copolymer | |
| CN115078707A (en) | Thin film biosensor based on magnetic force electric coupling and preparation method thereof | |
| US6238930B1 (en) | Layer structure for determination of analyte concentration based on micellar recognition system | |
| TC Moreira et al. | Sol-gel-based biosensing applied to medicinal science | |
| Li et al. | A label-free electrochemical bisphenol A immunosensor based on chlorogenic acid as a redox probe | |
| Gotoh et al. | Immuno-FET sensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191203 |