CN109010819B

CN109010819B - Application of recombinant attenuated listeria in preparation of cervical cancer therapeutic vaccine

Info

Publication number: CN109010819B
Application number: CN201810886597.2A
Authority: CN
Inventors: 汪川; 沈海浅
Original assignee: Nanjing Songyue Biotechnology Co ltd
Current assignee: Nanjing Songyue Biotechnology Co ltd
Priority date: 2018-08-06
Filing date: 2018-08-06
Publication date: 2022-05-10
Anticipated expiration: 2038-08-06
Also published as: CN109010819A

Abstract

The invention relates to an application of recombinant attenuated listeria in preparation of a vaccine for treating cervical cancer. The present invention uses attenuated Listeria monocytogenes (Lm for short) and attenuated sheep Listeria monocytogenes (Li for short) as carriers, and utilizes the unique characteristic of Listeria monocytogenes which can grow in host phagocyte cells and is a natural T cell immune activation adjuvant, so as to effectively improve the specific immune response in the tumor microenvironment and break the immune tolerance of the organism, thereby eliminating the persistent viral infection of the organism, and achieving the good treatment effect of eliminating the focus and inhibiting the tumor development. The method has the advantages that the defect of low immunogenicity of nucleic acid and protein polypeptide vaccines is overcome, and the attenuated Listeria keeps the characteristics that the original strain can grow in cells and complete antigen presentation and has higher safety.

Description

Application of recombinant attenuated listeria in preparation of cervical cancer therapeutic vaccine

Technical Field

The invention belongs to the technical field of biology, and relates to application of recombinant attenuated listeria in preparation of a vaccine for treating cervical cancer.

Background

Cervical cancer poses a serious threat to the health of women worldwide and is a gynecological malignancy with mortality second to breast cancer. Research shows that HPV is a main risk factor of cervical cancer, and more than 90 percent of cervical cancer patients are accompanied by high-risk HPV infection, wherein 16 types and 18 types are the main types. 90% of the cancer types of cervical cancer are cervical squamous carcinoma, and the main pathogenic type is HPV16 type. The main carcinogenic factors of HPV are E6 and E7 proteins, and E6 destroys normal cell cycle regulation by inhibiting the activity of a tumor suppressor p53, so as to promote cell immortalization; the E7 protein deprives pRb protein of its cancer-suppressing action by binding to and degrading it. The continuous high expression of E6 and E7 from the early stage of HPV infection and the expression only in the HPV infected tissues make them ideal targets for preparing cervical cancer therapeutic vaccines.

Compared with the traditional treatment means, such as surgical operation, radiotherapy and chemotherapy, the therapeutic vaccine has the characteristics of small damage and low toxicity. There are three main existing forms of therapeutic vaccines: protein polypeptide vaccines, viral (bacterial) vaccines and nucleic acid vaccines. The key to the success of the vaccine is to cause enough immune response, but the prior nucleic acid and protein polypeptide vaccine has low immunogenicity and obvious defects.

Disclosure of Invention

In view of the above, the present invention aims to provide the use of recombinant attenuated listeria in the preparation of vaccines for the treatment of cervical cancer.

In order to achieve the purpose, the invention provides the following technical scheme:

use of a recombinant attenuated Listeria monocytogenes or a recombinant attenuated Listeria ovis, which are obtained by carrying an HPV16 type E6E7 fusion epitope peptide group, as a vector, respectively, using an attenuated Listeria monocytogenes (hereinafter abbreviated as Lm) or an attenuated Listeria ovis (hereinafter abbreviated as Li) for the preparation of a vaccine for the treatment of cervical cancer.

Preferably, the attenuated listeria monocytogenes is obtained by completely knocking out two virulence genes, namely actA and plcB, of the listeria monocytogenes.

Preferably, the attenuated listeria ovis is obtained by completely knocking out two virulence genes, namely actA and plcB, of the listeria ovis.

Preferably, the prime-boost administration of the vaccine should use a different recombinant attenuated listeria.

Further preferably, the effective component of the vaccine for priming is recombinant attenuated listeria monocytogenes, and the effective component of the vaccine for boosting is recombinant attenuated listeria ovis or recombinant attenuated listeria monocytogenes.

Preferably, the administration mode of the vaccine is a first injection and at least two boosting injections, and the recombinant attenuated listeria monocytogenes and the recombinant attenuated listeria ovis are sequentially and alternately injected. The number of booster injections is determined according to the needs of the patient.

Preferably, the recombinant attenuated listeria monocytogenes is prepared by the following method:

(1) synthesizing HPV16 type E6E7 fusion antigen epitope peptide gene fragments;

(2) constructing a targeting plasmid;

(3) preparing competent cells Lm delta actAplcB-lacZ;

(4) performing electric transformation on the competent cells prepared in the step (3) by using the targeting plasmid prepared in the step (2);

(5) and performing homologous recombination, hybridization culture, screening and verification on Lm delta actAplcB-lacZ and the targeting plasmid.

Further preferably, the specific method of step (1) is: the HPV16 type E6 protein and E7 protein amino acid sequences are inquired from NCBI, then dominant T cell antigen epitopes are screened out and sequentially arranged through software (for example, CTLPred and NetMHC 4.0 software are used for analyzing possible CTL epitopes in the sequences, ProPred and NetMHCIIpan 3.1 software are used for analyzing possible Th epitopes in the sequences), then codon optimization is carried out according to Listeria preference codon tables to obtain optimized sequences, then HindIII enzyme digestion sites (AAGCTT) are added at the upstream, Xho I enzyme digestion sites (CTCGAG) are added at the downstream, and the designed sequences are finally directly obtained through synthesis. Wherein, the codon optimization method is realized by submitting the amino acid sequence to a website (such as http:// www.jcat.de /), and then selecting the Listeria monocytogenes preferred codon optimization option; the synthesis method of the DNA sequence comprises the following steps: the DNA sequence was synthesized directly using a gene synthesizer model ABI 3900-Thermo Fisher Scientific et al for a total of 866bp, as shown in SEQ ID NO. 1.

Further preferably, the specific method of step (2) is: the fusion epitope peptide gene fragment obtained in the step (1) is subjected to double enzyme digestion by using restriction enzymes Hind III and Xho I, meanwhile, the plasmid pCW203 (patent CN103074361B) is subjected to enzyme digestion by using the same restriction enzymes, the fusion epitope peptide gene fragment is inserted into enzyme digestion sites of pCW203Hind III and Xho I through technologies such as connection, transformation and the like, and the intermediate plasmid pCW203-E6E7 is obtained, and as shown in SEQ ID NO.2, the successful construction of the intermediate plasmid is verified through PCR verification, quality-improving particle verification and enzyme digestion verification. Cutting off a fragment between the BamH I enzyme cutting site and the Xho I enzyme cutting site of the intermediate plasmid pCW203-E6E7, and inserting the fragment between the BamH I enzyme cutting site and the Xho I enzyme cutting site of the recombinant plasmid pCW180 to obtain a targeting plasmid pCW180-E6E7 shown as SEQ ID No. 3; wherein the gene sequence of the recombinant plasmid pCW180 is shown as SEQ ID NO.13 in the sequence table.

Still more preferably, the recombinant plasmid pCW180 is prepared as follows:

(2-A) inserting an Lm mpl gene (Genbank ID: DQ054595.1) carrying speI at the upstream and Not I at the downstream into a space between speI and Not I enzyme cutting sites of a plasmid pCW154 (patent CN103074361B) to obtain a first intermediate recombinant plasmid pCW160, wherein the gene sequence of the first intermediate recombinant plasmid pCW160 is shown as SEQ ID NO.4 in a sequence table;

(2-B) inserting an Lm orfBAldh gene (Genbank ID: M82881.1) carrying XbaI at the upstream and Not I at the downstream into the XbaI and Not I enzyme cutting sites of the first intermediate recombinant plasmid pCW160 obtained in the step (2-A) to obtain a second intermediate recombinant plasmid pCW170, wherein the gene sequence of the second intermediate recombinant plasmid pCW170 is shown as SEQ ID NO.5 in the sequence table;

(2-C) digesting the plasmid pCW154 with NotI to obtain a section of gene with NotI digestion sites at two ends, and inserting the NotI digestion sites of the second intermediate recombinant plasmid pCW170 obtained in the step (2-B) to obtain a recombinant plasmid pCW 180; the gene sequence of the recombinant plasmid pCW180 is shown as SEQ ID NO.13 in the sequence table.

Further preferably, the specific method of step (3) is: lm delta actAplcB-lacZ was cultured in BHI broth containing 0.5mol/L sucrose, and A was periodically measured₆₀₀When A is₆₀₀When the concentration is 0.4, adding penicillin G to ensure that the final concentration is 12.5 mu G/ml, and continuing culturing; when A is₆₀₀Centrifuging when the concentration is 0.7, washing, subpackaging and storing.

More preferably, Lm Δ actA/plcB-lacZ is prepared as follows:

(3-A) inserting lacZ gene fragments (patent CN103074361B) with Not I enzyme cutting sites at the upstream and the downstream respectively into the Not I enzyme cutting sites of a second intermediate recombinant plasmid pCW170 to obtain a targeting plasmid pCW190, wherein the gene sequence of the plasmid pCW190 is shown as SEQ ID NO.6 in a sequence table;

and (3-B) electrically converting the targeting plasmid pCW190 obtained in the step (3-A) into Lm, and then carrying out single and double homologous recombination hybridization culture on the bacteria obtained by conversion to obtain the target plasmid.

Still more preferably, the specific method of step (3) is: inoculating Lm delta actAplcB-lacZ into 15ml BHI liquid culture medium containing 0.5mol/L sucrose, and culturing at 37 ℃ and 220rpm for 12-16 hours; the resulting bacterial liquid was transferred to 250ml of BHI broth containing 0.5mol/L sucrose, and A was periodically measured₆₀₀When A is₆₀₀When the concentration is 0.4, adding penicillin G to ensure that the final concentration is 12.5 mu G/ml, and continuing culturing; when A is₆₀₀When the concentration is 0.7, pouring the mixture into a 50mL centrifuge tube, centrifuging the mixture at 4 ℃ and 10000rpm for 5 minutes, and removing the supernatant; repeatedly washing the precipitate with 0.5mol/L ice-cold sucrose solution, adding 20ml 0.5mol/L sucrose solution each time, centrifuging at 4 deg.C 10000rpm for 10min, discarding the supernatant, and washing for 3 times; add 300. mu.l of resuspension of 0.5mol/L sucrose per tube, dispense pre-cooled sterile EP tubes at 50. mu.l/tube, store at-80 ℃.

Further preferably, the specific method of step (4) is: electrically transferring the targeting plasmid to Lm delta actAplcB-lacZ competent cells, and adding BHI broth to culture for 2 hours after the electric transfer is finished; the transformation liquid is coated on an erythromycin BHI agar plate, a single blue colony is screened out through blue-white spots, and plasmid extraction and PCR verification are carried out after pure culture.

Still more preferably, the specific method of step (4) is: taking out the prepared competent cells from a refrigerator at-80 deg.C, placing in an ice box, melting completely, slowly adding 5 μ l targeting plasmid into the competent cells Lm Δ actAplcB-lacZ at 1/10 ratio with a pre-cooling gun head at-20 deg.C, and making ddH₂O negative control; after mild mixing, carrying out ice bath for 5 minutes, then completely transferring into an electric rotating cup, carrying out ice bath for 5 minutes, and carrying out electric rotation in an electric rotating instrument; after the electric transfer is finished, ice-bath is carried out for 5 minutes, 750 mu l of BHI broth preheated at 37 ℃ is added by using a gun head preheated at 37 ℃, the mixture is uniformly mixed and then is completely transferred to an EP tube, and the mixture is cultured for 2 hours at 150rpm by a shaking table at 30 ℃; and (3) coating the whole amount of the bacterial liquid on a BHI agar plate (BHI-Ery-X-gal, BEXI plate for short) containing 1-5 mug/ml of erythromycin and 20-60 mug/ml of X-gal by using a sterile L-shaped glass rod, culturing for 48-72 hours at 30 ℃, then, carrying out pure culture on the blue bacterial colony on the BEXI plate, culturing for 24 hours at 30 ℃, carrying out plasmid extraction and PCR verification.

More preferably, the electrotransfer conditions are: the voltage is 1500V, the duration is 5ms, and the inner diameter of the electric revolving cup is 1 mm.

More preferably, the specific method for extracting the plasmid is as follows: extracting plasmids according to the specification of a plasmid extraction kit (Listeria is gram-positive bacteria, lysozyme is required to be added before Solution I is used, the final concentration of lysozyme is 20mg/L, the lysozyme is added with Solution I containing lysozyme, then the bacteria are resuspended, water bath is carried out for 45min at 37 ℃), and finally electrophoresis is carried out after Elution by 30 mu L of Solution Buffer; gel imaging is carried out, and whether a band exists or not is observed to judge whether the recombinant plasmid is successfully introduced, wherein the size of the plasmid is 9540 bp.

Still more preferably, the specific method for PCR verification is: extracting all genes in bacteria by a boiling method to be used as a template, amplifying a target band HPV 16E 6E7, and carrying out PCR amplification by using sequences shown in SEQ ID NO.7 and SEQ ID NO.8 as primers; the reaction cycle conditions were: 94 ℃ 3min → (94 ℃ 1min, 47 ℃ 30 sec, 72 ℃ 1min) × 30 cycles → 72 ℃ 10min → 4 ℃; the PCR results were observed electrophoretically, with the expected results: the fragment of HPV 16E 6E7 is about 852 bp.

The upstream primer HPV-f: 5'-CATCAAAAACGTACAGCAATG-3', as shown in SEQ ID NO. 7;

downstream primer HPV-r: 5'-TGGTTTTTGAGAACAAATTGG-3', as shown in SEQ ID NO. 8;

more preferably, the electrophoresis conditions are: 1% agarose gel; 90V, loading amount: 5 μ l of PCR product.

Further preferably, the specific method of step (5) is: continuously passaging Listeria monocytogenes carrying a targeting plasmid at 42 ℃ and 30 ℃, integrating a target fragment carried by the targeting plasmid into Listeria by utilizing a homologous recombination hybridization principle and a gene targeting technology, and screening out a suspicious strain by utilizing blue-white spots and erythromycin sensitivity; then PCR screening and gene sequencing verification are carried out.

More preferably, the specific method of subculture is: and (3) streaking and inoculating an Lm delta actAplcB-lacZ which is electrically transferred to a targeting plasmid into a BEXI plate, carrying out subculture at 42 ℃, selecting a single blue colony to inoculate BHI broth, carrying out subculture at 30 ℃, until the broth starts to grow at the 3 rd generation, adding BHI into each generation of culture to dilute, respectively coating 100 mu l of diluted bacterium liquid on the BEXI and BXI (BHI agar plate containing 20-60 mu g/ml of X-gal) plates, and carrying out culture at 30 ℃ for 24 hours.

More preferably, the specific method of subculture is: streaking and inoculating Lm delta actAplcB-lacZ which is electrically transferred into a targeting plasmid to a BEXI plate, subculturing for 2-3 generations at 42 ℃ (the culture time of each generation is 48 hours), selecting a single blue colony, inoculating 5ml of BHI broth, and subculturing for 6 generations at 30 ℃ and 200rpm (the culture time of each generation is 24 hours); starting at the 3 rd generation of the broth, 360. mu.l BHI broth (1:10 dilution) was added to 40. mu.l culture per generation and 1:10 dilutions were continued for several times until 10 was diluted⁶ Coating 10 times, respectively⁶Mu.l of the diluted bacterial solution was applied to BEXI and BXI plates and incubated at 30 ℃ for 24 hours; positive colonies Lm Δ actAplcB-HPV grew as white colonies on BXI plates and not on BEXI plates.

More preferably, the specific method of PCR screening is: and (3) amplifying the following three groups of gene fragments of the suspicious recombinant bacteria with correct screening results of the blue white spots and the erythromycin: anti-erythromycin gene, target antigen gene HPV 16E 6E7 and actA gene, and identifying corresponding vaccine strains.

More preferably, the specific method of PCR screening is: the bacterial genome extraction kit is used for extracting the genome DNA of Lm delta actAplcB-HPV as a template, and the following three groups of gene fragments are amplified: and identifying the recombinant bacterium Lm delta actAplcB-E6E7 according to the amplification result of the anti-erythromycin gene, the target antigen gene HPV 16E 6E7 and the actA gene.

More preferably, the sequence shown in SEQ ID NO.9 and SEQ ID NO.10 is used as a primer for PCR amplification of the erythromycin-resistant gene Ery; the reaction cycle conditions were: 94 ℃ 3min → (94 ℃ 1min, 60 ℃ 30s, 72 ℃ 2min) × 30 cycles → 72 ℃ 10min → 4 ℃; the PCR results were observed electrophoretically, with the expected results: the Ery gene fragment is about 1471 bp.

An upstream primer Ery-f: 5'-GATAAGTCGACGATTCACAAAAAATAG-3', as shown in SEQ ID NO. 9;

downstream primer Ery-r: 5'-AAAACTAGTCCCGGG GCGAATTG-3', as shown in SEQ ID NO. 10.

More preferably, the Lm actA gene takes the sequences shown in SEQ ID NO.11 and SEQ ID NO.12 as primers to carry out PCR amplification; the reaction cycle conditions were: 94 ℃ 3min → (94 ℃ 1min, 50 ℃ 30s, 72 ℃ 1min) × 30 cycles → 72 ℃ 10min → 4 ℃; the PCR results were observed electrophoretically, with the expected results: the Lm actA gene fragment is about 950 bp.

Upstream primer Lm-actA-f: 5'-GCTATAAATGAAGAGGCTTCAGG-3', as shown in SEQ ID NO. 11;

downstream primer Lm-actA-r: 5'-CTCTTAAATCAGCTAGGCGATC-3', as shown in SEQ ID NO. 12.

More preferably, the specific method for gene sequencing verification is as follows: identifying the correct corresponding vaccine strain by PCR, amplifying the target antigen gene HPV E6E7 by PCR with the genome DNA as a template, verifying the sequencing of the amplified product, and preserving the strain with the correct sequencing.

Preferably, the recombinant attenuated ovine listeria is prepared by the following method:

(1) synthesizing HPV16 type E6E7 fusion antigen epitope peptide gene fragments;

(2) constructing a targeting plasmid;

(3) preparing a competent cell Li delta actAplcB-lacZ;

(5) and performing homologous recombination hybridization culture and screening verification on the Li delta actAplcB-lacZ and the targeting plasmid.

Further preferably, the specific method of step (1) is: the HPV16 type E6 protein and E7 protein amino acid sequences are inquired from NCBI, then dominant T cell antigen epitopes are screened out and sequentially arranged through software (for example, CTLPred and NetMHC 4.0 software are used for analyzing possible CTL epitopes in the sequences, ProPred and NetMHCIIpan 3.1 software are used for analyzing possible Th epitopes in the sequences), then codon optimization is carried out according to Listeria preference codon tables to obtain optimized sequences, then HindIII enzyme digestion sites (AAGCTT) are added at the upstream, Xho I enzyme digestion sites (CTCGAG) are added at the downstream, and the designed sequences are finally directly obtained through synthesis. Wherein, the codon optimization method is realized by submitting the amino acid sequence to a website (such as http:// www.jcat.de /), and then selecting a sheep Listeria preferred codon optimization option; the synthesis method of the DNA sequence comprises the following steps: the DNA sequence was synthesized directly using a gene synthesizer model ABI 3900-Thermo Fisher Scientific et al for a total of 866bp, as shown in SEQ ID NO. 1.

Still more preferably, the specific method of step (2) is:

the fusion epitope peptide gene fragment obtained in the step (1) is subjected to double enzyme digestion by using restriction enzymes Hind III and Xho I, meanwhile, the plasmid pCW203 (patent CN103074361B) is subjected to enzyme digestion by using the same restriction enzymes, the fusion epitope peptide gene fragment is inserted into enzyme digestion sites of pCW203Hind III and Xho I through technologies such as connection, transformation and the like, and the intermediate plasmid pCW203-E6E7 is obtained, and as shown in SEQ ID NO.2, the successful construction of the intermediate plasmid is verified through PCR verification, quality-improving particle verification and enzyme digestion verification. The fragment between the BamH I cleavage site and the Xho I cleavage site of the intermediate plasmid pCW203-E6E7 was excised and inserted between the BamH I cleavage site and the Xho I cleavage site of the recombinant plasmid pCW154 (patent CN103074361B) to obtain the targeting plasmid pCW154-E6E7, as shown in SEQ ID NO. 14.

Further preferably, the specific method of step (3) is: li delta actAplcB-lacZ (patent CN103074361B) was cultured in BHI broth containing 0.5mol/L sucrose, and A was periodically measured₆₀₀When A is₆₀₀When the concentration is 0.4, adding penicillin G to ensure that the final concentration is 12.5 mu G/ml, and continuing culturing; when A is₆₀₀Centrifuging when the concentration is 0.7, washing, subpackaging and storing.

Still more preferably, the specific method of step (3) is: inoculating Li delta actAplcB-lacZ (patent CN103074361B) into 15ml BHI liquid culture medium containing 0.5mol/L sucrose, and culturing at 37 ℃ and 220rpm for 12-16 hours; the resulting bacterial liquid was transferred to 250ml of BHI broth containing 0.5mol/L sucrose, and A was periodically measured₆₀₀When A is₆₀₀When the concentration is 0.4, adding penicillin G to ensure that the final concentration is 12.5 mu G/ml, and continuing culturing; when A is₆₀₀When the temperature is 0.7, pouring the mixture into a 50mL centrifuge tube, centrifuging the mixture at 4 ℃ and 10000rpm for 5 minutes, and removing the supernatant; repeatedly washing the precipitate with 0.5mol/L ice-cold sucrose solution, adding 20ml of 0.5mol/L sucrose solution each time, centrifuging at 4 deg.C at 10000rpm for 10min, discarding the supernatant, and washing3 times; add 300. mu.l of resuspension of 0.5mol/L sucrose per tube, dispense pre-cooled sterile EP tubes at 50. mu.l/tube, store at-80 ℃.

Further preferably, the specific method of step (4) is: electrically transferring the targeting plasmid to a Li delta actAplcB-lacZ competent cell, and adding BHI broth to culture for 2 hours after the electric transfer is finished; the transformation liquid is coated on an erythromycin BHI agar plate, a single blue colony is screened out through blue-white spots, and plasmid extraction and PCR verification are carried out after pure culture.

Still more preferably, the specific method of step (4) is: taking out the prepared competent cells from a refrigerator at-80 deg.C, placing in an ice box, melting completely, slowly adding 5 μ l targeting plasmid into the competent cells Li Δ actAplcB-lacZ at 1/10 ratio with a precooling gun head at-20 deg.C, and making into ddH₂O negative control; after mild mixing, carrying out ice bath for 5 minutes, then completely transferring into an electric rotating cup, carrying out ice bath for 5 minutes, and carrying out electric rotation in an electric rotating instrument; after the electric transfer is finished, ice-bath is carried out for 5 minutes, 750 mu l of BHI broth preheated at 37 ℃ is added by using a gun head preheated at 37 ℃, the mixture is uniformly mixed and then is completely transferred to an EP tube, and the mixture is cultured for 2 hours at 150rpm by a shaking table at 30 ℃; and (3) coating the whole amount of the bacterial liquid on a BHI agar plate (BHI-Ery-X-gal, BEXI plate for short) containing 1-5 mug/ml of erythromycin and 20-60 mug/ml of X-gal by using a sterile L-shaped glass rod, culturing for 48-72 hours at 30 ℃, then, carrying out pure culture on the blue bacterial colony on the BEXI plate, culturing for 24 hours at 30 ℃, carrying out plasmid extraction and PCR verification.

More preferably, the specific method for extracting the plasmid is as follows: extracting plasmids according to the description of a plasmid extraction kit (the listeria is a gram-positive bacterium, lysozyme is required to be added before the Solution I is used, the final concentration of the lysozyme is 20mg/L, the lysozyme is added with the Solution I containing the lysozyme, then the bacteria are resuspended, the temperature is 37 ℃ in water bath for 45min), and finally electrophoresis is carried out after Elution by 30 mul of Elution Buffer; gel imaging is carried out, and whether a band exists or not is observed to judge whether the recombinant plasmid is successfully introduced, wherein the size of the plasmid is 9525 bp.

downstream primer HPV-r: 5'-TGGTTTTTGAGAACAAATTGG-3', as shown in SEQ ID NO. 8;

Further preferably, the specific method of step (5) is: continuously passaging sheep listeria carrying a targeting plasmid at 42 ℃ and 30 ℃, integrating a target fragment carried by the targeting plasmid into the listeria by utilizing a homologous recombination hybridization principle and a gene targeting technology, and screening out a suspicious strain by utilizing blue white spots and erythromycin sensitivity; then PCR screening and gene sequencing verification are carried out.

More preferably, the specific method of subculture is: and (3) streaking and inoculating a Li delta actAplcB-lacZ plate electrically transferred with a targeting plasmid into a BEXI plate, subculturing at 42 ℃, selecting a single blue colony to inoculate BHI broth, subculturing at 30 ℃, starting to transfer to the 3 rd generation of the broth, adding BHI into each generation of culture to dilute, respectively coating 100 mu l of diluted bacterium liquid on the BEXI and BXI (BHI agar plate containing 20-60 mu g/ml of X-gal) plates, and culturing for 24 hours at 30 ℃.

More preferably, the specific method of subculture is: inoculating a BEXI plate with the Li delta actAplcB-lacZ streaked line electrically transferred with the targeting plasmid, subculturing at 42 ℃ for 2-3 generations (the culture time of each generation is 48 hours), selecting a single blue colony, inoculating 5ml of BHI broth, and subculturing at 30 ℃ and 200rpm for 6 generations (the culture time of each generation is 24 hours); starting at the 3 rd generation of the broth, 360. mu.l BHI broth (1:10 dilution) was added to 40. mu.l culture per generation and 1:10 dilutions were continued for several times until 10 was diluted⁶ Coating 10 times, respectively⁶Mu.l of the diluted bacterial solution was applied to BEXI and BXI plates and incubated at 30 ℃ for 24 hours; positive bacterial colonyLi Δ actAplcB-E6E7 grew as white colonies on BXI plates and not on BEXI plates.

More preferably, the specific method of PCR screening is: extracting the genome DNA of Li delta actAplcB-E6E7 by using a bacterial genome extraction kit as a template, and amplifying the following three groups of gene fragments: and identifying the recombinant bacteria Li delta actAplcB-E6E7 according to the amplification result of the anti-erythromycin gene, the target antigen gene HPV 16E 6E7 and the actA gene.

More preferably, the Li actA gene takes sequences shown in SEQ ID NO.16 and SEQ ID NO.17 as primers for PCR amplification; the reaction cycle conditions were: 94 ℃ 3min → (94 ℃ 1min, 50 ℃ 30s, 72 ℃ 1min) × 30 cycles → 72 ℃ 10min → 4 ℃; the PCR results were observed electrophoretically, with the expected results: the Li actA gene fragment is about 600 bp.

An upstream primer Li-actA-f: 5'-GAAGCTAAAAGTGCAAATGTCCC-3', as shown in SEQ ID NO. 15;

downstream primer Li-actA-r: 5'-ATTTCTTTAATA CTGCGTTTGGGG-3', as shown in SEQ ID NO. 16.

The invention has the beneficial effects that:

the present invention uses attenuated Listeria monocytogenes (Lm for short) and attenuated sheep Listeria monocytogenes (Li for short) as carriers, and utilizes the unique characteristic of Listeria monocytogenes which can grow in host phagocyte cells and is a natural T cell immune activation adjuvant, so as to effectively improve the specific immune response in the tumor microenvironment and break the immune tolerance of the organism, thereby eliminating the persistent viral infection of the organism, and achieving the good treatment effect of eliminating the focus and inhibiting the tumor development. The method has the advantages that the defect of low immunogenicity of nucleic acid and protein polypeptide vaccines is overcome, and the attenuated Listeria keeps the characteristics that the original strain can grow in cells and complete antigen presentation and has higher safety. The method comprises the following specific steps:

1) the combined use of the attenuated listeria monocytogenes and the attenuated listeria ovis for multiple times of immunotherapy (like different combinations of twice of the attenuated listeria monocytogenes immunotherapy and 1 time of the attenuated listeria ovis immunotherapy) has obviously better treatment effect on cervical cancer than the effect of multiple times of immunotherapy (like three times of the attenuated listeria monocytogenes immunotherapy) on the same individual by using one bacterium, can more obviously reduce the tumor volume, even achieve the healing, and simultaneously prolong the life cycle.

2) Pertinence: inducing specific cell factor for cervical cancer cell, targeting treating cancer tissue, and no damage to normal cell.

3) High efficiency: the vaccine strain has high immunocompetence and induces efficient cell factor expression. The Listeria strain has the function of efficiently activating the cellular immune function of an organism, so that the recombinant strain carrying the antigen can activate various anti-tumor immune mechanisms, activate specific tumor killer T cells and break the effective tumor killing mechanism of immune suppression reactivation.

4) Safety: the attenuated strain does not exist in vivo for a long time, does not cause infection, shows good tolerance in preclinical tests, is proved to be safe by other similar clinical tests, and can be well compatible with other tumor treatment means, such as cocktail-type immunosuppressants, surgeries, radiotherapy and chemotherapy.

5) And (3) stabilizing: the antigen gene carried by the vaccine strain prepared by the invention is integrated into the genome of the strain, and the antigen gene stably exists in the strain and cannot be lost.

6) The cost is low: the vaccine is low in production cost, rapid and economical, and can be produced on a large scale by using a standard bacterial fermentation technology without cell culture, cold chain transportation and storage.

Drawings

In order to make the purpose, technical scheme and beneficial effect of the invention more clear, the invention provides the following drawings for explanation:

figure 1 is a map of Lm targeted recombinant plasmid structure. A structural map of Lm targeting plasmid contains two sections of Lm homologous genes (Lm mpl and Lm orfBAldh), ampicillin resistance gene (Amp), erythromycin resistance gene (Ery), gene cassette (gene cassette): comprises a promoter phly, an HA epitope gene, a VSV-G epitope gene, a GP33epitope gene, a GP61epitope gene and a fusion protein HPV 16E 6E7 gene.

FIG. 2 is a map of Li-targeted recombinant plasmid structures. A structural map of a Li targeting plasmid contains two Li homologous genes (Li mpl and Li orfBAldh), an ampicillin resistance gene (Amp), an erythromycin resistance gene (Ery), and a gene cassette (gene cassette): comprises a promoter phly, an HA epitope gene, a VSV-G epitope gene, a GP33epitope gene, a GP61epitope gene and a fusion protein HPV E6E7 gene.

FIG. 3 is a diagram showing the results of PCR identification electrophoresis after the target plasmid is electrically transformed into Listeria. For a PCR identification electrophoresis result picture after the electro-transformation of the targeting plasmid into Li delta actAplcB-lacZ and Lm delta actAplcB-lacZ, A figure 1 is a target fragment HPV 16E 6E7 amplified from bacteria after the electro-transformation of the targeting plasmid into Li delta actAplcB-lacZ, and M is DNA ladder; b FIG. 2 shows target fragment HPV 16E 6E7 amplified from bacteria after electrically converting Lm delta actAplcB-lacZ with targeting plasmid, M is DNAlader.

FIG. 4 is a diagram showing the identification result of the upgraded plasmid after the target plasmid is electrically transformed into Listeria. The method comprises the steps of performing electric transformation on a targeting plasmid to obtain a Li delta actAplcB-lacZ and an Lm delta actAplcB-lacZ plasmid extraction result, wherein 1 is a verification result of quality improvement grains from the Li delta actAplcB-lacZ after the electric transformation, 2 is a verification result of quality improvement grains from the Lm delta actAplcB-lacZ after the electric transformation, and M is a DNA ladder.

FIG. 5 is a diagram showing the result of PCR screening of recombinant bacteria. The results of the PCR identification electrophoresis of Li Δ actAplcB-E6E7 and Lm Δ actAplcB-E6E7 show that + is a positive control, that-is a negative control, and that M is a DNA ladder. A: 1 is used for amplifying HPV 16E 6E7 by taking a Li delta actAplcB-E6E7 genome as a template, and 2 is used for amplifying HPV 16E 6E7 by taking an Lm delta actAplcB-E6E7 genome as a template; b: 1 is used for amplifying an anti-erythromycin gene by taking a Li delta actAplcB-E6E7 genome as a template, and 2 is used for amplifying an anti-erythromycin gene by taking an Lm delta actAplcB-E6E7 genome as a template; c: 1 is an actA gene amplified by taking a Li delta actAplcB-E6E7 genome as a template; d: 1 is the amplification of the actA gene using Lm delta actAplcB-E6E7 genome as template. The PCR amplification result accords with expectation, which indicates that the two recombinant vaccine strains are successfully constructed, and the HPV E6E7 gene segment in the targeting plasmid is integrated into the genome of the bacteria at a fixed point.

FIG. 6 is a schematic diagram showing the structures of foreign genes inserted into the genomes of recombinant bacteria Lm.DELTA.actaaplcB-E6E 7(A) and Li.DELTA.actaaplcB-E6E 7(B) and the genes before and after the insertion. From this figure, it was found that the insertion site of the foreign gene was deleted the actA and plcB genes from the recombinant bacterial genome. The inserted foreign gene expresses fusion protein under the action of self-contained promoter phly, and the fusion protein HAs secretion signal peptide, GP33 and GP61epitope peptide tags and HA and VSV-G western blot detection tags.

FIG. 7 is a survival curve of the C57BL/6 inoculated recombinant vaccine strain. C57BL/6 mice were inoculated with different doses of Lm Δ actAplcB-E6E7 or Li Δ actAplcB-E6E7 in the tail vein, and survival of the mice was observed for 10 days after inoculation to plot survival curves of the dose groups. The inoculation dose of Lm delta actAplcB-E6E7 was 2.7X 10⁷CFU/only, 2.4X 10⁸CFU/only and 2.6X 10⁹CFU/mouse, survival curve as shown in 7 a; the inoculation dose of Li delta actAplcB-E6E7 was 3.0X 10⁷CFU/only, 3.3X 10⁸CFU/only and 3.5X 10⁹CFU/min, survival curves are shown in 7 b. According to the modified Kouyan methodCalculated Lm delta actAplcB-E6E7 LD₅₀Is 1.3X 10⁸LD of CFU/Li Δ actAplcB-E6E7₅₀Is 4 x 10⁸CFU/only.

FIG. 8C 57BL/6 cervical cancer model mice tumor volumes at different time points following tail vein vaccine strain immunotherapy. C57BL/6 mice were inoculated with 1X 10 TC-1 cells subcutaneously on the right flank of day 0⁵One for each model, three immunotherapies were performed on the modeled mice on

days

7, 14 and 21 after TC-1 cell inoculation, and the tumor volumes of each group of mice were recorded. In the figure, a is the tumor volume of each group of model mice on day 7 after TC-1 cell inoculation; b, c, d are tumor volumes at different time points after immunotherapy in each group of model mice. Wherein A is a physiological saline control group (NS → NS → NS group), and B is a Li carrier bacterium control group (Li delta actAplcB-lacZ → Li delta actAplcB-lacZ group); c is an Lm vector bacterium control group (Lm delta actAplcB-lacZ → Lm delta actAplcB-lacZ group), D is a treatment group 1(Li delta actAplcB-E6E7 → Li delta actAplcB-E6E7 → Li delta actAplcB-E6E7 group), E is a treatment group 2(Lm delta actAplcB-E6E7 → Lm delta AplcB-E6E7 → Lm delta actlcB-E6E 7 group), F is a treatment group 3(Li delta actlcB-E6E 7 → Lm delta actlcB-E6E 7 Li → delta actlcB-E6E 7 group), and G is a treatment group 4 (Li delta actlcB-E6E 7 → Lm delta actlcB-E6E 7 → Li actlcB-E7 group). There was no statistical difference in tumor volume among the groups of mice at the time of the first immunotherapy (day 7). After the second (14 th day) and the third tumor treatment (21 th day), observation is carried out on the 42 th day (when the tumor length of the mouse is 20mm, the mouse is killed), no mouse survives in the normal saline control group (A), the Li carrier bacterium control group (B) and the Lm carrier bacterium control group (C), and the tumor size of the survived mouse in each treatment group is effectively controlled, wherein 1 mouse in the treatment group 1(D) is cured, 2 mice in the treatment group 2(E) is cured, 3 mice in the treatment group 3(F) are cured, and 4 mice in the treatment group 4(G) are cured.

FIG. 9 shows the survival of the cervical cancer model mice in each group after tail vein injection immunotherapy. Survival of mice of each group after three immunotherapies on

days

7, 14 and 21 after TC-1 cell inoculation of cervical cancer model mice, wherein A is a saline physiological control group (NS → NS → NS group), and B is a Li carrier bacterium control group (Li Δ actAplcB-lacZ → Li Δ actAplcB-lacZ → Li Δ actAplcB-lacZ); c is an Lm vector bacterium control group (Lm delta actAplcB-lacZ → Lm delta actAplcB-lacZ group), D is a treatment group 1(Li delta actAplcB-E6E7 → Li delta actAplcB-E6E7 → Li delta actAplcB-E6E7 group), E is a treatment group 2(Lm delta actAplcB-E6E7 → Lm delta AplcB-E6E7 → Lm delta actlcB-E6E 7 group), F is a treatment group 3(Li delta actlcB-E6E 7 → Lm delta actlcB-E6E 7 Li → delta actlcB-E6E 7 group), and G is a treatment group 4 (Li delta actlcB-E6E 7 → Lm delta actlcB-E6E 7 → Li actlcB-E7 group). The survival analysis of the control group and the treated group was performed, and the normal saline control group (a) was compared with the treated group 1(D) (P < 0.05), the Li vehicle control group (B) was compared with the treated group 1(D) (P < 0.05), the normal saline control group (a) was compared with the treated group 2(E) (P < 0.001), the Lm vehicle control group (C) was compared with the treated group 2(E) (P < 0.001), the normal saline control group (a) was compared with the treated group 3(F) (P < 0.001), and the control group was compared with the treated group 4(G) (P < 0.001), each of the treated groups significantly prolonged the survival time of the mice of the cervical cancer model, wherein the treated

groups

2, 3, 4 were dead later than the treated group 1, and the treated groups 3, 4 had higher survival rates than the treated

groups

1, 2 at the observation time nodes.

FIG. 10 is a photograph of a tumor of a 35d mouse which is a cervical cancer model. A is a saline physiological control group (NS → NS → NS group), B is a Li carrier bacterium control group (Li delta actAplcB-lacZ → Li delta actAplcB-lacZ group); c is an Lm vector bacterium control group (Lm delta actAplcB-lacZ → Lm delta actAplcB-lacZ group), D is a treatment group 1(Li delta actAplcB-E6E7 → Li delta actAplcB-E6E7 → Li delta actAplcB-E6E7 group), E is a treatment group 2(Lm delta actAplcB-E6E7 → Lm delta AplcB-E6E7 → Lm delta actlcB-E6E 7 group), F is a treatment group 3(Li delta actlcB-E6E 7 → Lm delta actlcB-E6E 7 Li → delta actlcB-E6E 7 group), and G is a treatment group 4 (Li delta actlcB-E6E 7 → Lm delta actlcB-E6E 7 → Li actlcB-E7 group).

Detailed Description

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Example 1 preparation of the vaccine bacterium Li Δ actAplcB-E6E7

(1) HPV 16E 6E7 gene fragment synthesis

The method comprises the steps of inquiring HPV16 type E6 protein and E7 protein genes from NCBI, predicting dominant T cell epitope by online software analysis, selecting and combining fusion antigen epitope peptide genes, carrying out corresponding optimization according to Listeria codons, adding HindIII enzyme cutting sites at the upstream and Xho I enzyme cutting sites at the downstream, and finally directly obtaining a DNA sequence (obtained in a form of providing a cloning plasmid pUC57-E6E7 by a company) through synthesis, wherein the fragment length is 866bp (the sequence is shown as a sequence 1 in a sequence table).

(2) Construction of targeting plasmids

Construction of the intermediate plasmid pCW203-E6E7

Plasmids pUC57-E6E7 and pCW203 were extracted according to the plasmid instructions and eluted with 30. mu.L of Elution Buffer.

pUC57-E6E7 and pCW203 were double digested with Hind III and XhoI, 20. mu.L: pUC57-E6E7 or pCW203 < 1ng, Hind III 1. mu.L, XhoI 1. mu.L, 10 XNEB Buffer 2. mu.L, ddH₂O make up the system to 20. mu.L. The plasmid is digested in water bath at 37 ℃ for 1h, 0.5 mu L of CIAP is added into the vector fragment, and dephosphorylation is carried out in water bath at 37 ℃ for 30 min. Mixing the enzyme digestion mixture with 6 × Loading Buffer, electrophoresing (1% agarose, 94V) and gelating to recover fragments with corresponding length (pUC57-E6E7 recovers small fragment after enzyme digestion, namely fusion antigen epitope peptide gene fragment with the length of 866 bp; pCW203 recovers large fragment after enzyme digestion, namely vector fragment with the length of about 866 bp)>10Kb), eluted with 30. mu.L of Elution Buffer.

Respectively taking fusion epitope peptide antigen gene fragments and carrier fragments according to a connection system: vector 50ng, insert moles: vector fragment molar ═ 5:1, T4Ligase 1. mu.L, 10 XLigase Buffer 5. mu.L, ddH₂O make up the system to 10. mu.l and attach for 1h at 22 ℃. The ligation product was gently mixed with competent cells of E.coli DH5 α at a volume of 1:10, ice-cooled for 30min, heat shocked at 42 ℃ for 45s, ice-cooled for 3min, and added with 500. mu.L of preheated SOB broth, mixed well, cultured at 37 ℃ and 180rpm for 1h, spread on LA plates (LB-Amp plates: LB plates containing 100. mu.g/mL Amp), and cultured at 37 ℃ for 16-20 h.

And (3) PCR screening: using bacterial DNA genome extracted by boiling method as template, amplifying target band HPV 16E 6E7, primer HPV-f/r (f: 5'-CATCAAAAACGTACAGCAATG-3', r: 5'-TGGTTTTTGAGAACAAATTGG-3'), reaction circulation condition is: 94 ℃ 3min → (94 ℃ 1min, 47 ℃ 30s, 72 ℃ 1min) × 30 cycles → 72 ℃ 10min → 4 ℃; electrophoretic Observation of PCR results (electrophoresis conditions: 1% agarose gel; 90V, loading: 5. mu.l PCR product), expected results: the fragment of HPV 16E 6E7 is about 852 bp.

Enzyme digestion verification: plasmids were extracted from positive bacteria screened and verified by PCR, digested with Hind III and Xho I according to the above digestion system, and the digested mixture was mixed with 6 × Loading Buffer for electrophoresis (1% agarose, 94V).

Sequencing and verifying: and extracting the plasmid which is expected by PCR and enzyme digestion verification, and sending the plasmid to a sequencing company for sequencing. Coli carrying the positive plasmid, which was completely correct after sequencing verification, was stored at-80 ℃.

(ii) construction of targeting plasmid

Plasmid pCW154 was extracted and eluted with 30. mu.L of Elution Buffer. Plasmid pCW154 and intermediate plasmid pCW203-E6E7 are respectively and uniformly mixed by restriction enzymes BamH I and Xho I according to the system of (2) (HindIII is replaced by BamH I), enzyme digestion and dephosphorylation are carried out, gel is recovered after electrophoresis, and small fragments after enzyme digestion of pCW203-E6E7, namely an insert fragment (931bp) and a pCW154 vector skeleton (long fragment after enzyme digestion, the length is about 8606 bp). And (3) uniformly mixing the systems according to the step (2), connecting, converting into escherichia coli DH5 alpha, coating the escherichia coli DH5 alpha on an LA plate, and performing PCR screening, BamHI and XhoI double-enzyme digestion verification and sequencing verification on a single growing colony, wherein the specific operation is the same as the step (2) (replacing HindIII enzyme with BamHI enzyme). The E.coli carrying the positive plasmid after sequencing verification was stored at-80 ℃. The structural map of the targeting plasmid is shown in FIG. 2.

(3) Preparation of Li delta actAplcB-lacZ competent cell for electrotransformation

Inoculating Li delta actAplcB-lacZ into 15ml BHI liquid culture medium containing 0.5mol/L sucrose, and culturing at 37 ℃ and 220rpm for 12-16 h. The resulting bacterial liquid was transferred to 250ml of BHI broth containing 0.5mol/L sucrose, and A was periodically measured₆₀₀When A is₆₀₀When the concentration was 0.4, penicillin G was added to a final concentration of 12.5. mu.g/ml, and the culture was continued. When A is₆₀₀When the concentration is 0.7, the mixture is poured into a 50mL centrifuge tube, centrifuged at 10000rpm at 4 ℃ for 5min, and the supernatant is discarded. The precipitate was washed repeatedly with 0.5mol/L ice-cold sucrose solution20ml of 0.5mol/L sucrose solution is added each time, the mixture is centrifuged at 10000rpm at 4 ℃ for 10min, and the supernatant is discarded and washed for 3 times. Add 300. mu.l of resuspension of 0.5mol/L sucrose per tube, dispense pre-cooled sterile EP tubes at 50. mu.l/tube, store at-80 ℃.

(4) Electrotransformation of targeting plasmid into Li delta actAplcB-lacZ

Taking out the prepared competent cells from a refrigerator at-80 deg.C, placing in an ice box, melting completely, slowly adding 5 μ l targeting plasmid into the competent cells Li Δ actAplcB-lacZ at 1/10 ratio with a precooling gun head at-20 deg.C, and making into ddH₂And (4) O negative control. After mild mixing, the mixture is iced for 5min, then the mixture is completely transferred into an electric rotating cup, the ice-cooling is carried out for 5min, and the electric rotating is carried out in an electric rotating instrument (the electric rotating condition is that the voltage is 1500V, the duration is 5ms, and the inner diameter of the electric rotating cup is 1 mm). After the completion of the electrotransfer, the mixture was ice-cooled for 5min, 750. mu.l of BHI broth preheated at 37 ℃ was added to the flask with the tip preheated at 37 ℃, the mixture was mixed well and transferred to an EP tube, and the mixture was cultured for 2 hours at 150rpm with a shaker at 30 ℃. And (3) coating the bacterial liquid on a BHI agar plate (BHI-Ery-X-gal, BEXI plate) containing 1-5 mug/ml of erythromycin and 20-60 mug/ml of X-gal by using a sterile L-shaped glass rod in a full amount, culturing at 30 ℃ for 48-72 hours, wherein the positive bacteria are blue colonies, performing pure culture on the BEXI plate, culturing at 30 ℃ for 24 hours, and performing PCR verification and quality-improved particle verification, wherein the steps are shown in a figure 3 and a figure 4.

And (3) PCR verification: extracting all genes of bacteria by a boiling method as a template, amplifying a target band E6E7 and a primer HPV-f/r (f: 5'-CATCAAAAACGTACAGCAATG-3', r: 5'-TGGTTTTTGAGAACAAATTGG-3'), wherein the reaction circulation conditions are as follows: 94 ℃ 3min → (94 ℃ 1min, 47 ℃ 30s, 72 ℃ 1min) × 30 cycles → 72 ℃ 10min → 4 ℃; electrophoretic Observation of PCR results (electrophoresis conditions: 1% agarose gel; 90V, loading: 5. mu.l PCR product), expected results: the fragment of HPV 16E 6E7 is about 852 bp. As shown in FIG. 3, it was suggested that the targeting plasmid had been transferred into the bacterium.

Extracting plasmid according to the plasmid extraction kit (Listeria is gram-positive bacteria, lysozyme is required to be added before Solution I is used, the final concentration of lysozyme is 20mg/L, the bacteria are resuspended after the Solution I containing lysozyme is added, water bath at 37 ℃ is carried out for 45min), and finally electrophoresis is carried out after Elution by 30 mul of Elution Buffer. Gel imaging is carried out, and whether a band exists or not is observed to judge whether the recombinant plasmid is successfully introduced, wherein the size of the plasmid is 9525 bp. As shown in FIG. 4, the plasmid extraction was successful, and the length was as expected, indicating that the targeting plasmid was successfully transferred.

(5) Homologous recombination and screening of Li delta actAplcB-lacZ and targeting plasmid

Homologous recombination hybridization culture of Li delta actAplcB-lacZ and targeting plasmid

Li delta actAplcB-lacZ streaked BEXI plate with electrically transferred targeting plasmid was subcultured at 42 deg.c for 2-3 passages (48 hr per passage), and a single blue colony was inoculated into 5ml BHI broth and subcultured at 30 deg.c and 200rpm for 6 passages (24 hr per passage). At the beginning of the 3 rd passage of the broth, 40. mu.l of the culture was diluted 1:10 with 360. mu.l of BHI and 10 serial dilutions were made⁶ Coating 10 times, respectively⁶Mu.l of the diluted bacterial solution was plated on BEXI and BXI (BHI agar plates containing X-gal 20-60. mu.g/ml) and cultured at 30 ℃ for 24 hours. The positive colony Li Δ actAplcB-E6E7 grew as a white colony on BXI plates and did not grow on BEXI plates.

② the Li delta actAplcB-E6E7 identified by PCR

Extracting the genome DNA of Li delta actAplcB-E6E7 by using the bacterial genome extraction kit as a template, and amplifying the following three groups of gene fragments: and identifying the recombinant bacteria Li delta actAplcB-E6E7 according to the amplification result of the anti-erythromycin gene, the target antigen gene cassette and the actA gene. As shown in FIG. 5, 1 in A is HPV 16E 6E7 amplified by taking Li delta actAplcB-E6E7 genome DNA as a template, the primers and the reaction conditions are shown in (2), 1 in B is an anti-erythromycin gene Ery of an amplification target band, which is about 1471bp, the primers Ery-f/r (f: 5'-GATAAGTCGACGATTCACAAAAAATAG-3', r: 5'-AAAACTAGTCCCGGG GCGAATTG-3'), and the reaction cycle conditions are as follows: 94 ℃ 3min → (94 ℃ 1min, 60 ℃ 30s, 72 ℃ 2min) × 30 cycles → 72 ℃ 10min → 4 ℃; 1 in C is amplified Li actA gene, about 600bp, a primer Li-actA-f/r (f: 5'-GAAGCTAAAAGTGCAAATGTC CC-3', r: 5'-ATTTCTTTAATACTGCGTTTGGGG-3'), and the reaction circulation conditions are as follows: 94 ℃ 3min → (94 ℃ 1min, 55 ℃ 30s, 72 ℃ 1min) × 30 cycles → 72 ℃ 10min → 4 ℃; . The Li delta actAplcB-E6E7 carries an E6E7 fusion gene and does not carry an anti-erythromycin gene, and the actA gene is knocked out through PCR identification. The identification result is consistent with the expectation, and Li delta actAplcB-E6E7 is successfully prepared.

Third Gene sequencing verification

After PCR identification, using bacterial genome DNA as a template, carrying out PCR amplification on a target antigen gene HPV 16E 6E7, carrying out sequencing verification on an amplification product, and carrying out strain preservation on a strain with correct sequencing. The structural schematic diagram of the foreign gene inserted into the recombinant bacterial genome and the genes before and after the insertion site is shown in FIG. 6.

(6) Candidate strain LD of Li delta actAplcB-E6E7 vaccine₅₀Measurement of (2)

After thawing the Li.DELTA.actaaplcb-E6E 7 strain stored at-20 ℃ in a water bath at 37 ℃, 10. mu.l of each strain was inoculated into 5ml of BHI broth and cultured overnight at 200rpm at 37 ℃. On the next day, 40. mu.l of the once-recovered bacterial suspension was taken and cultured in 20ml of BHI broth at 37 ℃ and 200rpm overnight for two recoveries.

Inoculating 17.5ml of each of the twice recovered bacterial solutions to 350ml of BHI broth, culturing at 37 ℃ and 200rpm by shaking until A is obtained₆₀₀When the value is between 0.3 and 0.7, determining A at the moment according to the growth curve of Listeria₆₀₀The corresponding bacterial quantity is calculated, and concentration or dilution treatment is carried out according to the predicted bacterial inoculation dose: taking a certain amount of bacterial liquid for centrifugation, carrying out 13000rpm for 2min, carrying out re-centrifugation after re-suspension by using normal saline, then re-suspending the bacterial liquid to the bacterial infection dosage by using the normal saline, and placing the bacterial liquid on ice for standby.

After 6-8 weeks old C57BL/6 female mice are adaptively fed for 3 days, the mice are randomly divided into 3 groups, 7 mice in each group are inoculated with 3.0 multiplied by 10 tail veins respectively⁷CFU/only, 3.3X 10⁸CFU/only and 3.5X 10⁹CFU/only; continuously observing for 10 days, counting the death and survival conditions of the mice, and drawing survival curves of each dose group, as shown in figure 7 b; calculating LD by using improved kouzhui method₅₀And at 0.1 × LD₅₀As the optimum inoculation amount.

Example 2 preparation of Miao bacterium Lm.DELTA.actaAplcB-E6E 7

(1) HPV 16E 6E7 gene fragment synthesis

Same as example 1(1)

(2) Construction of targeting plasmids

(ii) construction of the intermediate plasmid pCW203-E6E7 the same as in example 1(2) (ii)

(ii) construction of targeting plasmid

Plasmid pCW180 was extracted and eluted with 30. mu.L of Elution Buffer. Plasmid pCW180 and intermediate plasmid pCW203-E6E7 are mixed with restriction enzymes BamH I and Xho I respectively according to the system of (2) (HindIII is replaced by BamH I), and the mixture is cut and dephosphorylated, and the gel is recovered after electrophoresis, and the cut small fragments of pCW203-E6E7, namely the insert (931bp) and the pCW180 vector skeleton (long fragment after cut, length is about 8621 bp). The system according to (2) is mixed uniformly, connected, transformed into escherichia coli DH5 alpha, coated on an LA plate, and subjected to PCR screening, BamHI and XhoI double enzyme digestion verification and sequencing verification on a single growing colony, which is specifically performed in the same manner as in (2) (HindIII enzyme is replaced by BamHI enzyme). The E.coli carrying the positive plasmid after sequencing verification was stored at-80 ℃. The structural map of the targeting plasmid is shown in FIG. 1.

(3) Preparation of Lm delta actAplcB-lacZ competent cell for electrotransformation

This is the same as example 1(3), where Li Δ actAplcB-lacZ is changed to Lm Δ actAplcB-lacZ.

(4) Electrically converting targeting plasmid into Lm delta actAplcB-lacZ

This step is similar to example 1(4), in which the targeting plasmid was changed to pCW180-E6E7, and the plasmid size was 9540 bp; li delta actAplcB-lacZ was changed to Lm delta actAplcB-lacZ. The PCR verification result of the bacteria after electrotransformation is shown in figure 3, and the extraction verification result of the bacteria plasmid after electrotransformation is shown in figure 4.

(5) Lm delta actAplcB-lacZ and targeting plasmid homologous recombination and screening

Lm delta actAplcB-lacZ and targeting plasmid homologous recombination hybridization culture

This is done in step (1), (5) of example, where Li Δ actAplcB-lacZ is replaced by Lm Δ actAplcB-lacZ.

② the PCR identification of Lm delta actAplcB-E6E7

The bacterial genome extraction kit extracts genome DNA of Lm delta actAplcB-E6E7 as a template, and carries out amplification of the following three groups of gene fragments: and identifying the recombinant bacterium Lm delta actAplcB-E6E7 according to the amplification result of the anti-erythromycin gene, the target antigen gene cassette and the actA gene. As shown in FIG. 5, 2 in A is fusion epitope peptide antigen gene E6E7 amplified by using Lm delta actAplcB-E6E7 genome DNA as a template, the primer and the reaction condition are shown in example 1(2), 2 in B is the amplified band anti-erythromycin gene Ery with about 1471bp, primer Ery-f/r (f: 5'-GATAAGTCGACGATTCACAAAAAATAG-3', r: 5'-AAAACTAGTCCCGGGGCGAATTG-3'), and the reaction cycle condition is as follows: 94 ℃ 3min → (94 ℃ 1min, 60 ℃ 30s, 72 ℃ 2min) × 30 cycles → 72 ℃ 10min → 4 ℃; in D, 1 is amplified Lm actA gene, about 950bp, primer Lm-actA-f/r (f: 5'-GCTATAAATGAAGAGGCTTCAGG-3', r: 5'-CTCTTAAATCAGCTAGGCGATC-3'), and the reaction cycle conditions are as follows: 94 ℃ 3min → (94 ℃ 1min, 50 ℃ 30s, 72 ℃ 1min) × 30 cycles → 72 ℃ 10min → 4 ℃. Through PCR identification, the Lm delta actAplcB-E6E7 carries the E6E7 fusion epitope peptide gene, does not carry the anti-erythromycin gene, and the actA gene is removed. The identification result is consistent with the expectation, and shows that the construction of Lm delta actAplcB-E6E7 is successful.

Third Gene sequencing verification

This step is similar to example 1 (5).

(6) Determination of Lm delta actAplcB-E6E7 vaccine candidate strain LD50

This step is the same as in example 1 (6). Wherein Lm delta actAplcB-E6E7 immunization groups are inoculated with 2.7 multiplied by 10 tail veins respectively⁷CFU/only, 2.7X 10⁸CFU/only and 2.6X 10⁹CFU/only.

Example 3: evaluation of therapeutic effect of vaccine strain on cervical cancer

(1) TC-1 tumor cell culture and cervical cancer model establishment

Using RPMI1640Medium containing 10% fetal calf serum, 1% penicillin and streptomycin at 37 deg.C with 5% CO₂Culturing TC-1 cells in an incubator until the density reaches about 80%, rinsing the cells with Hank's solution for 2-3 times, digesting with pancreatin, counting with PBS (phosphate buffer solution) in a heavy suspension manner, and preparing the cells into the concentration of 1 × 10⁶The cell suspension of the mice is 100 mu l, and the cell suspension is injected into the right flank of the C57BL/6 female mouse with the age of 6-8 weeks, so that a mouse HPV infection tumor model is established, and the tumor can be formed in a macroscopic view after about 7 days. Tumor volume according to formula 1/2 × (a × b)²) Calculate (a, b are the longest and shortest diameters of the tumor, respectively). Statistical analysis of the data was performed using SPSS software. Mean ratio of two samplesPreferably by t test, P<A difference of 0.05 is statistically significant. As shown in FIG. 8a, the tumor volumes were uniform among the groups of mice, and there were no statistical differences among the groups.

(2) Immunotherapy of modeled mice

The experiment establishes a negative control group: injecting Normal Saline (NS); control group of Li-carrier bacteria: li delta actAplcB-lacZ is injected for the first time, and Li delta actAplcB-lacZ is injected for the second time in an enhanced mode; performing three times of intensive injection on Li delta actAplcB-lacZ; lm vector control group: first injecting Lm delta actAplcB-lacZ, second boosting injecting Lm delta actAplcB-lacZ, and third boosting injecting Lm delta actAplcB-lacZ; treatment groups: treatment group 1: first Li delta actAplcB-E6E7, second boost Li delta actAplcB-E6E7, and third boost Li delta actAplcB-E6E 7; treatment group 2: first Lm delta actAplcB-E6E7 injection, second Lm delta actAplcB-E6E7 injection, and third Lm delta actAplcB-E6E7 injection; treatment group 3: the first Li delta actAplcB-E6E7 injection, the second Lm delta actAplcB-E6E7 intensive injection and the third Li delta actAplcB-E6E7 intensive injection; treatment group 4: first injection of Lm Δ actAplcB-E6E7, second boost injection of Li Δ actAplcB-E6E7, and third boost injection of Lm Δ actAplcB-E6E 7.

77 molded mice were randomly divided into 7 groups of 11 mice each. The first immunotherapy was performed on day 7 after the injection of TC-1 cells, and the bacterial suspension was prepared according to example 1(6), and 100. mu.l of each strain was inoculated into the tail vein, and an equal volume of NS was inoculated into the tail vein of the NS control group. A second booster treatment was given on day 14 and a third booster treatment was given on day 21, at the same method and dose as the first. Mice body weight, tumor growth and survival were observed and recorded every 2 days according to formula 1/2 × (a × b)²) Tumor volumes were calculated (a, b are the longest and shortest diameters of the tumor, respectively). Statistical analysis of the data was performed using SPSS software. The mean value comparison of two samples adopts t test, the life cycle comparison adopts logarithmic rank test, P<A difference of 0.05 is statistically significant. The results are shown in FIGS. 8, 9 and 10, in which A is a saline control group (NS → NS → NS group) and B is a Li-carrying bacterium control group (Li Δ actAplcB-lacZ → Li Δ actAplcB-lacZ → Li Δ actAplcB-lacZ group); c is Lm vector bacterium control group (Lm delta actAplcB-lacZ → Lm delta actAplcB-lac)Group Z), group D was treatment group 1 (group Li Δ actaaplcb-E6E 7 → group Li Δ actaaplcb-E6E 7 → group Li Δ actaaplcb-E6E 7), group E was treatment group 2 (group Lm Δ actaaplcb-E6E 7 → Lm Δ actaaplcb-E6E 7 → group Lm Δ actaaplcb-E6E 7), group F was treatment group 3 (group Li Δ actaaplcb-E6E 7 → Lm Δ actaaplcb-E6E 7 → group Li Δ aplcb-E6E 7), and group G was treatment group 4 (group Lm Δ actaaplcb-E6E 7 → group Li Δ actaaplcb-E6E 7 → group Lm Δ actalb-E6E 7). Figure 8 shows that there was no statistical difference in tumor volume between groups of mice at the first immunotherapy (day 7). After the second (14 th day) and the third (21 th day) tumor treatment, the 42 th day (when the tumor length of the mouse is 20mm, the mouse is killed), no mouse survives in the normal saline control group, the Li carrier bacterium control group and the Lm carrier bacterium control group, and the tumor size of the surviving mouse in each treatment group is effectively controlled, wherein 1 mouse in the treatment group 1 is cured, 2 mice in the treatment group 2 are cured, 3 mice in the treatment group 3 are cured, and 4 mice in the treatment group 4 are cured. FIG. 9 is a graph showing survival curves of mice in each group, wherein the survival analysis of the control group compared with each treatment group was performed, and the survival of saline compared with the treatment group 1 (P < 0.05), the Li vehicle control group compared with the treatment group 1 (P < 0.05), the saline control group compared with the treatment group 2 (P < 0.001), the Lm vehicle control group compared with the treatment group 2 (P < 0.001), the saline control group compared with the treatment group 3 (P < 0.001), and the control group compared with the treatment group 4 (P < 0.001), and the survival time of mice in cervical cancer model was significantly prolonged in each treatment group, wherein the

treatment groups

2, 3, 4 died later than the treatment group 1, and the treatment groups 3, 4 had higher survival rates than the

treatment groups

1, 2 at the observation time nodes. Experimental results show that the prepared vaccine strain has effective control and treatment effects on cervical cancer, and the effect of vaccine strain combination for multiple immunotherapy is superior to that of single vaccine strain for multiple immunotherapy.

Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.

Sequence listing

<110> Nanjing Songyue Biotech Co., Ltd

Application of <120> recombinant attenuated listeria in preparation of cervical cancer therapeutic vaccine

<160> 16

<170> SIPOSequenceListing 1.0

<210> 1

<211> 866

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 1

aagctttgca tcaaaaacgt acagcaatgt tccaagatcc acaagaatct ggtcgtaaat 60

taccacaatt atgtacagaa ttacaaacaa caattcatga tattatttta gaatgtgttt 120

attgtaaaca acaattatta cgtcgtgaag tttatgattt cgcattccgt gatttatgta 180

ttgtttatcg tgatggtaac ccatatgcag tttgtgataa atgtttaaaa ttctattcta 240

aaatttctga atatcgtcat catggtgata caccaacatt acatgaatat atgttagatt 300

tacaaccaga aacaacagat ttatatggtt atggtcaatt aaacgattct tctgaagaag 360

aagatgaaat tgatggtcca gcaggtcaag cagaaccaga tcgtgcacat tataacattg 420

ttacattctg ttgtaaatgt gattctacat tagataaatg tttaaaattc tattctaaaa 480

tttctgaata tcgtcattat tgttattctg tttatggtac aacattagaa caacaatata 540

acaaaccatt atgtgattta ttaattcgtt gtattaactg tcaaaaacca ttatgtccag 600

aagaaaaaca acgtcattta gataaaaaac aacgtttcca taacattcgt ggtcgttgga 660

caggtcgttg tatgtcttgt tgtcgttctt ctcgtacacg tcgtgaaaca caattacatt 720

ataacattgt tacattctgt tgtaaatgtg attctacatt acgtttatgt gttcaatcta 780

cacatgttga tattcgtaca ttagaagatt tattaatggg tacattaggt attgtttgtc 840

caatttgttc tcaaaaacca ctcgag 866

<210> 2

<211> 13913

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 2

tgcggccgcc agtgtgatgg atatctgcag aattaattcg gctttctaga gtgactttta 60

tgttgaggca ttaacatttg ttaacgacga taaagggaca gcaggactag aataaagcta 120

taaagcaagc atataatatt gcgtttcatc tttagaagcg aatttcgcca atattataat 180

tatcaaaaga gaggggtggc aaacggtatt tggcattatt aggttaaaaa atgtagaagg 240

agagtgaaac ccatgaaaaa aataatgcta gtttttatta cacttatatt agttagtcta 300

ccaattgcgc aacaaactga agcaaaggat gcatcggatc catatccata tgatgttcca 360

gattatgcat cattgaattc aatgagaagt gaaagaccac aagctttgca tcaaaaacgt 420

acagcaatgt tccaagatcc acaagaatct ggtcgtaaat taccacaatt atgtacagaa 480

ttacaaacaa caattcatga tattatttta gaatgtgttt attgtaaaca acaattatta 540

cgtcgtgaag tttatgattt cgcattccgt gatttatgta ttgtttatcg tgatggtaac 600

ccatatgcag tttgtgataa atgtttaaaa ttctattcta aaatttctga atatcgtcat 660

catggtgata caccaacatt acatgaatat atgttagatt tacaaccaga aacaacagat 720

ttatatggtt atggtcaatt aaacgattct tctgaagaag aagatgaaat tgatggtcca 780

gcaggtcaag cagaaccaga tcgtgcacat tataacattg ttacattctg ttgtaaatgt 840

gattctacat tagataaatg tttaaaattc tattctaaaa tttctgaata tcgtcattat 900

tgttattctg tttatggtac aacattagaa caacaatata acaaaccatt atgtgattta 960

ttaattcgtt gtattaactg tcaaaaacca ttatgtccag aagaaaaaca acgtcattta 1020

gataaaaaac aacgtttcca taacattcgt ggtcgttgga caggtcgttg tatgtcttgt 1080

tgtcgttctt ctcgtacacg tcgtgaaaca caattacatt ataacattgt tacattctgt 1140

tgtaaatgtg attctacatt acgtttatgt gttcaatcta cacatgttga tattcgtaca 1200

ttagaagatt tattaatggg tacattaggt attgtttgtc caatttgttc tcaaaaacca 1260

ctcgagtata cagatattga aatgaataga ttaggaaaat gataagtcga cgattcacaa 1320

aaaataggca cacgaaaaac aagttaaggg atgcagttta tgcatccctt aacttactta 1380

ttaaataatt tatagctatt gacaagagat aagaattgtt caaagctaat attgtttaaa 1440

tcgtcaattc ctgcatgttt taaggaattg ttaaattgat tttttgtaaa tattttcttg 1500

tattctttgt taacccattt cagaacgaaa taattatact tttgtttatc tttgtgtgat 1560

attcttgatt tttttctact taatctgata agtgagctat tcactttagg tttaggatga 1620

aaatattctc ttggaaccat acttaatata gaaatatcaa cttctgccat taaaagtaat 1680

gccaatgagc gttttgtatt taataatctt ttagcaaacc cgtattccac gattaaataa 1740

atctcattag ctatactatc aagaacaatt ttgcgtatta tatccgtact tatgttataa 1800

ggtatattac catatatttt ataggattgg tttttaggaa atttaaactg caatatatcc 1860

ttgtttaaaa cttggaaatt atcgtgatca acaagtttat tttctgtagt tttgcataat 1920

ttatggtcta tttcaatggc agttacgaaa ttacacctct ttactaattc aagggtaaaa 1980

tggccttttc ctgagccgat ttcaaagata ttatcatgtt catttaatct tatatttgtc 2040

attattttat ctatattatg ttttgaagta ataaagtttt gactgtgttt tatatttttc 2100

tcgttcatta taaccctctt taatttggtt atatgaattt tgcttattaa cgattcatta 2160

taaccactta ttttttgttt ggttgataat gaactgtgct gattacaaaa atactaaaaa 2220

tgcccatatt ttttcctcct tataaaatta gtataattat agcacgagct ctgataaata 2280

tgaacatgat gagtgatcgt taaatttata ctgcaatcgg atgcgattat tgaataaaag 2340

atatgagaga tttatctaat ttcttttttc ttgtaaaaaa agaaagttct taaaggtttt 2400

atagttttgg tcgtagagca cacggtttaa cgacttaatt acgaagtaaa taagtctagt 2460

gtgttagact ttatgaaatc tttatacgtt tatatatatt tattatccgg aggtgtagca 2520

tgtctcattc aattttgagg gttgccagag ttaaaggatc aagtaataca aacgggatac 2580

aaagacataa tcaaagagag aataaaaact ataataataa agacataaat catgaggaaa 2640

catataaaaa ttatgatttg attaacgcac aaaatataaa gtataaagat aaaattgatg 2700

aaacgattga tgagaattat tcagggaaac gtaaaattcg gtcagatgca attcgccccg 2760

ggactagtgc ggccgctaat aatcttgcgc ttcgatgaca acagctgtac cagatgcagt 2820

gaccattagc attccgttat cagctccaag cacttcataa tcaatatcaa caccgataac 2880

ggcatttgcg ccaatatctt tcgcacgttg ttccatttca cgaattgctt cctcgcgagc 2940

attaataagt tcatcttcat agccttgcga acggcccccg aagaaatttc gaagtccagc 3000

cccaatatct ttcataaagt taacgccagt gatgacttcg ccgaaaacga tttttttata 3060

ttcgataatt tgtttgcctt caatatttgg tgaagttgtt acaatcatga gttatcccta 3120

cagtttttct tttatcatac ctcttagtac tttttctagt caaaggatat ccggttattt 3180

cgtacgattt cgcgcttttt ctatataaga aatagcatct ggaactttac aagctgtatt 3240

tccaaggttt acatgaactt tcccgactga tttcgcggct tccatcgctt tttcgtgcaa 3300

atcgtctttg taaattccac aagtgataat aaaaccgttc attgaatagc gggttctatt 3360

ggtttctgtt tgcagcgttt ctttcgcgcg ttgaagtaat gtctctgctt caggcaaatt 3420

gttaatatgt tcgctgtaaa tctgccagcc catggattta gttaaatcat aatcactttc 3480

aatccatgtt ttggcgaaaa ggagtgcatc atcacgtttg cttacaatcg atgctagtcc 3540

aaaagttagt tgtgaccatg tttcgcgaaa agcgatattc catttttcga tttgttcggt 3600

ggttattttg tttggattaa ttgctagcaa accgagataa atcaagtcac tattatttga 3660

ttcaatcagt tttacggcga gttcgtgatt ttttgtcagt ttttctcggc ggatgatttt 3720

ctttaaatca ccaattttta gtccgtaaag atctaatgaa tccggacaac cgtgattacg 3780

aaaaattttg atcgtattgg ggttttctaa ggcttgtagc tcggtgtcaa gttggtcaaa 3840

agtaatcata cgcgtcactc ctctcgaata aagtaagtat aacaaaaaaa gcatgcgaag 3900

gcgcatgctt taggatttaa gaatattagt ctatttgttt cattgcgtcg tctagctaga 3960

attaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 4020

ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 4080

atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 4140

taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 4200

cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 4260

aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 4320

aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 4380

tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc 4440

ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 4500

catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 4560

aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 4620

ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 4680

gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc 4740

aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 4800

gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 4860

gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 4920

gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 4980

gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 5040

gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 5100

atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 5160

ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 5220

ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 5280

ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 5340

ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 5400

ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 5460

tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 5520

tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 5580

tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 5640

tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 5700

gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 5760

tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 5820

ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 5880

gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 5940

gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt 6000

acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 6060

gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc 6120

cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 6180

cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 6240

caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac 6300

agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct 6360

ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc acttgatgcc 6420

tccgtgtaag ggggaatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat 6480

gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa 6540

acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg 6600

cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat 6660

ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa 6720

accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca 6780

cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag 6840

ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc 6900

cgacgatgat aagctgtcaa acatgagaat taattcccga ttatgtcttt tgcgcactcg 6960

gcttaaacca gttttcgctg gtgcgaaaaa agagtgtctt gtgacaccta aattcaaaat 7020

ctatcggtca gatttatacc gatttgattt tatatattct tgaataacat acgccgagtt 7080

atcacataaa agcgggaacc aatcatcaaa tttaaacttc attgcataat ccattaaact 7140

cttaaattct acgattcctt gttcatcaat aaactcaatc atttctttaa ttaatttata 7200

tctatctgtt gttgttttct ttaataattc atcaacatct acaccgccat aaactatcat 7260

atcttctttt tgatatttaa atttattagg atcgtccatg tgaagcatat atctcacaag 7320

acctttcaca cttcctgcaa tctgcggaat agtcgcattc aattcttctg ttattatttt 7380

tatctgttca taagatttat taccctcata catcactaga atatgataat gctctttttt 7440

catcctacct tctgtatcag tatccctatc atgtaatgga gcactacaaa ttgaatgtgt 7500

aactctttta aatactctaa ccactcggct ttgctgattc tggatataaa acaaatgtcc 7560

aattacgtcc tcttgaattt ttcttgtttt cagtttcttt tattacattt tcgctcatga 7620

tataataacg gtgctaatac acttaacaaa atttagtcat agataggcag catgccagtg 7680

ctgtctatct ttttttgttt aaaatgcacc gtattcctcc tttgcatatt tttttattag 7740

aataccggtt gcatctgatt tgctaatatt atatttttct ttgattctat ttaatatctc 7800

attttcttct gttgtaagtc ttaaagtaac agcaactttt ttctcttctt ttctatctac 7860

aactatcact gtacctccca acatctgttt ttttcacttt aacataaaaa acaacctttt 7920

aacattaaaa acccaatatt tatttatttg tttggacaat ggacaatgga cacctagggg 7980

ggaggtcgta gtacccccct atgttttctc ccctaaataa ccccaaaaat ctaagaaaaa 8040

aagacctcaa aaaggtcttt aattaacatc tcaaatttcg catttattcc aatttccttt 8100

ttgcgtgtga tgcgctgcgt ccattaaaaa tcctagagct ttgcaaccga aagttaatag 8160

ctgtcgctac tactttcgct tacgctctaa gtatatttta aggactgtca cacgcaaaaa 8220

gttttctcgg cataaaagta cctctacatc tctaaatcgt ctgtacgctg tttctcacgc 8280

tttctatcga tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc 8340

atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc 8400

ctgactgcgt tagcaattta actgtgataa actaccgcat taaagctagc tttaaggcta 8460

aatgccgaat ggttggcacc taccgcattg gcaaccgtgg cagaagaggg cgcatccgtt 8520

ttggcgaaaa agagtaaaac ggcgaggatg agtgcacagc cagagcccag ccagaaaaca 8580

aactgattat tgatggtgaa catgatgccg acaatcgagg cacacagcgc ccagccaaca 8640

cagccaaaca tccgcgcgcg accaaattcg aaattactgc gacggctgac tttctcaata 8700

aatgcctcta ctgctggcgc accggcgtta aaacaaaagc ctagataaat accaccaaca 8760

atcgatccta ctaaaatgtt gtattgtaac agtggcccga agataaaaat aaagaacggc 8820

gcaaacatca ctaacatgcc ggtaataatc cacagcaggt atttgcgcag cccgagtttg 8880

tcagaaagca gaccaaacag cggttggaat aatagcgaga acagagaaat agcggcaaaa 8940

ataatacccg tatcactttt gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac 9000

gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag 9060

tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg cagacatggc 9120

ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg accggcgctc 9180

agctggaatt aattccgccg atactgacgg gctccaggag tcgtcgccac caatccccat 9240

atggaaaccg tcgatattca gccatgtgcc ttcttccgcg tgcagcagat ggcgatggct 9300

ggtttccatc agttgctgtt gactgtagcg gctgatgttg aactggaagt cgccgcgcca 9360

ctggtgtggg ccataattca attcgcgcgt cccgcagcgc agaccgtttt cgctcgggaa 9420

gacgtacggg gtatacatgt ctgacaatgg cagatcccag cggtcaaaac aggcggcagt 9480

aaggcggtcg ggatagtttt cttgcggccc taatccgagc cagtttaccc gctctgctac 9540

ctgcgccagc tggcagttca ggccaatccg cgccggatgc ggtgtatcgc tcgccacttc 9600

aacatcaacg gtaatcgcca tttgaccact accatcaatc cggtaggttt tccggctgat 9660

aaataaggtt ttcccctgat gctgccacgc gtgagcggtc gtaatcagca ccgcatcagc 9720

aagtgtatct gccgtgcact gcaacaacgc tgcttcggcc tggtaatggc ccgccgcctt 9780

ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct tcacttacgc caatgtcgtt 9840

atccagcggt gcacgggtga actgatcgcg cagcggcgtc agcagttgtt ttttatcgcc 9900

aatccacatc tgtgaaagaa agcctgactg gcggttaaat tgccaacgct tattacccag 9960

ctcgatgcaa aaatccattt cgctggtggt cagatgcggg atggcgtggg acgcggcggg 10020

gagcgtcaca ctgaggtttt ccgccagacg ccactgctgc caggcgctga tgtgcccggc 10080

ttctgaccat gcggtcgcgt tcggttgcac tacgcgtact gtgagccaga gttgcccggc 10140

gctctccggc tgcggtagtt caggcagttc aatcaactgt ttaccttgtg gagcgacatc 10200

cagaggcact tcaccgcttg ccagcggctt accatccagc gccaccatcc agtgcaggag 10260

ctcgttatcg ctatgacgga acaggtattc gctggtcact tcgatggttt gcccggataa 10320

acggaactgg aaaaactgct gctggtgttt tgcttccgtc agcgctggat gcggcgtgcg 10380

gtcggcaaag accagaccgt tcatacagaa ctggcgatcg ttcggcgtat cgccaaaatc 10440

accgccgtaa gccgaccacg ggttgccgtt ttcatcatat ttaatcagcg actgatccac 10500

ccagtcccag acgaagccgc cctgtaaacg gggatactga cgaaacgcct gccagtattt 10560

agcgaaaccg ccaagactgt tacccatcgc gtgggcgtat tcgcaaagga tcagcgggcg 10620

cgtctctcca ggtagcgaaa gccatttttt gatggaccat ttcggcacag ccgggaaggg 10680

ctggtcttca tccacgcgcg cgtacatcgg gcaaataata tcggtggccg tggtgtcggc 10740

tccgccgcct tcatactgca ccgggcggga aggatcgaca gatttgatcc agcgatacag 10800

cgcgtcgtga ttagcgccgt ggcctgattc attccccagc gaccagatga tcacactcgg 10860

gtgattacga tcgcgctgca ccattcgcgt tacgcgttcg ctcatcgccg gtagccagcg 10920

cggatcatcg gtcagacgat tcattggcac catgccgtgg gtttcaatat tggcttcatc 10980

caccacatac aggccgtagc ggtcgcacag cgtgtaccac agcggatggt tcggataatg 11040

cgaacagcgc acggcgttaa agttgttctg cttcatcagc aggatatcct gcaccatcgt 11100

ctgctcatcc atgacctgac catgcagagg atgatgctcg tgacggttaa cgcctcgaat 11160

cagcaacggc ttgccgttca gcagcagcag accattttca atccgcacct cgcggaaacc 11220

gacatcgcag gcttctgctt caatcagcgt gccgtcggcg gtgtgcagtt caaccaccgc 11280

acgatagaga ttcgggattt cggcgctcca cagtttcggg ttttcgacgt tcagacgtag 11340

tgtgacgcga tcggcataac caccacgctc atcgataatt tcaccgccga aaggcgcggt 11400

gccgctggcg acctgcgttt caccctgcca taaagaaact gttacccgta ggtagtcacg 11460

caactcgccg cacatctgaa cttcagcctc cagtacagcg cggctgaaat catcattaaa 11520

gcgagtggca acatggaaat cgctgatttg tgtagtcggt ttatgcagca acgagacgtc 11580

acggaaaatg ccgctcatcc gccacatatc ctgatcttcc agataactgc cgtcactcca 11640

acgcagcacc atcaccgcga ggcggttttc tccggcgcgt aaaaatgcgc tcaggtcaaa 11700

ttcagacggc aaacgactgt cctggccgta accgacccag cgcccgttgc accacagatg 11760

aaacgccgag ttaacgccat caaaaataat tcgcgtctgg ccttcctgta gccagctttc 11820

atcaacatta aatgtgagcg agtaacaacc cgtcggattc tccgtgggaa caaacggcgg 11880

attgaccgta atgggatagg ttacgttggt gtagatgggc gcatcgtaac cgtgcatctg 11940

ccagtttgag gggacgacga cagtatcggc ctcaggaaga tcgcactcca gccagctttc 12000

cggcaccgct tctggtgccg gaaaccaggc aaagcgccat tcgccattca ggctgcgcaa 12060

ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 12120

atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 12180

aacgacggga tccataaaaa ctagcattat ttttttcatg ggtttcactc tccttctaca 12240

ttttttaacc taataatgcc aaataccgtt tgccacccct ctcttttgat aattataata 12300

ttggcgaaat tcgcttctaa agatgaaacg caatattata tgcttgcttt atagctttat 12360

tctagtcctg ctgtcccttt atcgtcgtta acaaatgtta atgcctcaac ataaaagtca 12420

ctctagtaca tttttatctc atttttataa tcctgataga gataatactt atttgccggg 12480

ttttgctaat gcgaaaataa caggagcaaa gtatttcaat caatcggtga ctgattaccg 12540

agaagggaaa tttgacacag cgttttataa attaggccta gcaatccatt attatacgga 12600

tattagtcaa cctatgcacg ccaataattt taccgcaata tcataccctc caggctacca 12660

ctgtgcatat gaaaattacg tagataccat taaacacaat tatcaagcaa cggaagacat 12720

ggtagcaaaa agattttgct cagatgacgt gaaagactgg ctctatgaaa atgcgaaaag 12780

ggcgaaagcg gactacccga aaatagtcaa tgcgaaaact aaaaaatcat atttagtagg 12840

aaattctgaa tggaaaaagg atacagtgga acctactgga gctagactaa gagattcaca 12900

gcaaactttg gcaggttttt tagaattttg gtctaaaaaa acaaatgaat aacaatattt 12960

aggaatacat tcttatccac tcgttagcgg gtggatatat tttatgggga ggaagtaagc 13020

caaatgtata taaaagggag gttaatcttt ttctttgtag tgttagtaat cgcgttatgt 13080

tccgtattaa tattgcttat aataaaaata agcgtatgga aagatgaacc atttcattta 13140

agcgatgcaa aggagattga gtgtcttgga agttgtgaaa taaaaaatac taatcaaaaa 13200

atccatttct tctccataaa agagaatttg tttgaggaaa agggtgatat agctggaatt 13260

ttaaatgaag acgaacaaaa agttgctgat aagtccattt tcattgttat tttggatgat 13320

gaaaaaggga ttgcgaacga agagtaaagc ggtttgttag gtagtcttga atagttaaaa 13380

aacaatatag gaggaacatc tatgaaaatg cttaaaaagg gtaccgcagt tctatttgta 13440

atgatcatgg cagttatgtt agtcgcgtgt ggggataaag aagaaacgaa aacttacaca 13500

ctttcccaaa atggtgttga ttctaaatta acatacacat ataagggaga caaagttact 13560

aaacaaacag ctgaaaatac aatgtcatat gcttcgttag gtgtggcttc aaaagaagac 13620

gctgaaaaaa tgcttaaagc aacaagcgat aaattccaag gtattgatgg tttaaaagaa 13680

aagattgaat ataaagatga taaagctatt gaaacactag aagtagatta cactaaaatc 13740

tcttcggaag atatgaacaa aatccctggt atgtcttcaa atggtgatac ttctaaaggg 13800

attagcatgg aggaatctgc aaaaatgcta gaatcccaag gctataaaga agtttcaaaa 13860

taaatttgca ataaaaaaga agtatccgat tcatttggat acttcttttt tat 13913

<210> 3

<211> 9540

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 3

gaattcccga ttatgtcttt tgcgcactcg gcttaaacca gttttcgctg gtgcgaaaaa 60

agagtgtctt gtgacaccta aattcaaaat ctatcggtca gatttatacc gatttgattt 120

tatatattct tgaataacat acgccgagtt atcacataaa agcgggaacc aatcatcaaa 180

tttaaacttc attgcataat ccattaaact cttaaattct acgattcctt gttcatcaat 240

aaactcaatc atttctttaa ttaatttata tctatctgtt gttgttttct ttaataattc 300

atcaacatct acaccgccat aaactatcat atcttctttt tgatatttaa atttattagg 360

atcgtccatg tgaagcatat atctcacaag acctttcaca cttcctgcaa tctgcggaat 420

agtcgcattc aattcttctg ttattatttt tatctgttca taagatttat taccctcata 480

catcactaga atatgataat gctctttttt catcctacct tctgtatcag tatccctatc 540

atgtaatgga gcactacaaa ttgaatgtgt aactctttta aatactctaa ccactcggct 600

ttgctgattc tggatataaa acaaatgtcc aattacgtcc tcttgaattt ttcttgtttt 660

cagtttcttt tattacattt tcgctcatga tataataacg gtgctaatac acttaacaaa 720

atttagtcat agataggcag catgccagtg ctgtctatct ttttttgttt aaaatgcacc 780

gtattcctcc tttgcatatt tttttattag aataccggtt gcatctgatt tgctaatatt 840

atatttttct ttgattctat ttaatatctc attttcttct gttgtaagtc ttaaagtaac 900

agcaactttt ttctcttctt ttctatctac aactatcact gtacctccca acatctgttt 960

ttttcacttt aacataaaaa acaacctttt aacattaaaa acccaatatt tatttatttg 1020

tttggacaat ggacaatgga cacctagggg ggaggtcgta gtacccccct atgttttctc 1080

ccctaaataa ccccaaaaat ctaagaaaaa aagacctcaa aaaggtcttt aattaacatc 1140

tcaaatttcg catttattcc aatttccttt ttgcgtgtga tgcgctgcgt ccattaaaaa 1200

tcctagagct ttgcaaccga aagttaatag ctgtcgctac tactttcgct tacgctctaa 1260

gtatatttta aggactgtca cacgcaaaaa gttttctcgg cataaaagta cctctacatc 1320

tctaaatcgt ctgtacgctg tttctcacgc tttctatcga tcccgcaaga ggcccggcag 1380

taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat 1440

gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa 1500

actaccgcat taaagcttgt cgacgattca caaaaaatag gcacacgaaa aacaagttaa 1560

gggatgcagt ttatgcatcc cttaacttac ttattaaata atttatagct attgacaaga 1620

gataagaatt gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa 1680

ttgttaaatt gattttttgt aaatattttc ttgtattctt tgttaaccca tttcagaacg 1740

aaataattat acttttgttt atctttgtgt gatattcttg atttttttct acttaatctg 1800

ataagtgagc tattcacttt aggtttagga tgaaaatatt ctcttggaac catacttaat 1860

atagaaatat caacttctgc cattaaaagt aatgccaatg agcgttttgt atttaataat 1920

cttttagcaa acccgtattc cacgattaaa taaatctcat tagctatact atcaagaaca 1980

attttgcgta ttatatccgt acttatgtta taaggtatat taccatatat tttataggat 2040

tggtttttag gaaatttaaa ctgcaatata tccttgttta aaacttggaa attatcgtga 2100

tcaacaagtt tattttctgt agttttgcat aatttatggt ctatttcaat ggcagttacg 2160

aaattacacc tctttactaa ttcaagggta aaatggcctt ttcctgagcc gatttcaaag 2220

atattatcat gttcatttaa tcttatattt gtcattattt tatctatatt atgttttgaa 2280

gtaataaagt tttgactgtg ttttatattt ttctcgttca ttataaccct ctttaatttg 2340

gttatatgaa ttttgcttat taacgattca ttataaccac ttattttttg tttggttgat 2400

aatgaactgt gctgattaca aaaatactaa aaatgcccat attttttcct ccttataaaa 2460

ttagtataat tatagcacga gctctgataa atatgaacat gatgagtgat cgttaaattt 2520

atactgcaat cggatgcgat tattgaataa aagatatgag agatttatct aatttctttt 2580

ttcttgtaaa aaaagaaagt tcttaaaggt tttatagttt tggtcgtaga gcacacggtt 2640

taacgactta attacgaagt aaataagtct agtgtgttag actttatgaa atctttatac 2700

gtttatatat atttattatc cggaggtgta gcatgtctca ttcaattttg agggttgcca 2760

gagttaaagg atcaagtaat acaaacggga tacaaagaca taatcaaaga gagaataaaa 2820

actataataa taaagacata aatcatgagg aaacatataa aaattatgat ttgattaacg 2880

cacaaaatat aaagtataaa gataaaattg atgaaacgat tgatgagaat tattcaggga 2940

aacgtaaaat tcggtcagat gcaattcgcc ccgggactag ttaaaggtgg agaaattgat 3000

tcgtttgtcc attatggctt gaattgcaat aatgcctttt gggatggcca agaaattctt 3060

tatggagatg gggacaaaaa gaatttcaaa ccattttcat gcgccaaaac tattgttggt 3120

catgaactaa cgcatgcagt tatccagtat tcggcgggat tggaatacga agggcaatca 3180

ggtgcgctaa acgagtcgtt cgccgatgtt tttggttatt ttattgcgcc aaatcattgg 3240

ttgattggtg aggatgtctg tgtgcgtggg tcgcgagatg ggcgaataag aagcattaaa 3300

gatcctgaca aatataatca agcggctcat atgaaggatt acgaatcgct tccaatcaca 3360

gaggaaggcg actggggcgg agttcattat aatagtggta tcccgaataa agcagcctat 3420

aatactatca ctaaacttgg aaaagaaaaa acagaacagc gacgatttcg cgccttaaag 3480

tactatttaa cgaaaaaagc ccagtttacc gatgcgaaaa aagcgcttca acaagcagcg 3540

aaagatttat atggtgaaga tgcttctaaa aaagttgctg aagcttggga agcggtagga 3600

gttaactgag cggccgccag tgtgatggat atctgcagaa ttaattcggc tttctagagt 3660

gacttttatg ttgaggcatt aacatttgtt aacgacgata aagggacagc aggactagaa 3720

taaagctata aagcaagcat ataatattgc gtttcatctt tagaagcgaa tttcgccaat 3780

attataatta tcaaaagaga ggggtggcaa acggtatttg gcattattag gttaaaaaat 3840

gtagaaggag agtgaaaccc atgaaaaaaa taatgctagt ttttattaca cttatattag 3900

ttagtctacc aattgcgcaa caaactgaag caaaggatgc atcggatctt aaagctgttt 3960

ataattttgc tactatgaag gatccatatc catatgatgt tccagattat gcatcattga 4020

attcaatgag aagtgaaaga ccacaagctt tgcatcaaaa acgtacagca atgttccaag 4080

atccacaaga atctggtcgt aaattaccac aattatgtac agaattacaa acaacaattc 4140

atgatattat tttagaatgt gtttattgta aacaacaatt attacgtcgt gaagtttatg 4200

atttcgcatt ccgtgattta tgtattgttt atcgtgatgg taacccatat gcagtttgtg 4260

ataaatgttt aaaattctat tctaaaattt ctgaatatcg tcatcatggt gatacaccaa 4320

cattacatga atatatgtta gatttacaac cagaaacaac agatttatat ggttatggtc 4380

aattaaacga ttcttctgaa gaagaagatg aaattgatgg tccagcaggt caagcagaac 4440

cagatcgtgc acattataac attgttacat tctgttgtaa atgtgattct acattagata 4500

aatgtttaaa attctattct aaaatttctg aatatcgtca ttattgttat tctgtttatg 4560

gtacaacatt agaacaacaa tataacaaac cattatgtga tttattaatt cgttgtatta 4620

actgtcaaaa accattatgt ccagaagaaa aacaacgtca tttagataaa aaacaacgtt 4680

tccataacat tcgtggtcgt tggacaggtc gttgtatgtc ttgttgtcgt tcttctcgta 4740

cacgtcgtga aacacaatta cattataaca ttgttacatt ctgttgtaaa tgtgattcta 4800

cattacgttt atgtgttcaa tctacacatg ttgatattcg tacattagaa gatttattaa 4860

tgggtacatt aggtattgtt tgtccaattt gttctcaaaa accactcgag ggattaaatg 4920

gaccagatat ttataaagga gtttatcaat ttaaaagtgt tgaatttgat gtcgagtata 4980

cagatattga aatgaataga ttaggaaaat gataagtcgg cggccgctaa taatcttgcg 5040

cttcgatgac aacagctgta ccagatgcag tgaccattag cattccgtta tcagctccaa 5100

gcacttcata atcaatatca acaccgataa cggcatttgc gccaatatct ttcgcacgtt 5160

gttccatttc acgaattgct tcctcgcgag cattaataag ttcatcttca tagccttgcg 5220

aacggccccc gaagaaattt cgaagtccag ccccaatatc tttcataaag ttaacgccag 5280

tgatgacttc gccgaaaacg atttttttat attcgataat ttgtttgcct tcaatatttg 5340

gtgaagttgt tacaatcatg agttatccct acagtttttc ttttatcata cctcttagta 5400

ctttttctag tcaaaggata tccggttatt tcgtacgatt tcgcgctttt tctatataag 5460

aaatagcatc tggaacttta caagctgtat ttccaaggtt tacatgaact ttcccgactg 5520

atttcgcggc ttccatcgct ttttcgtgca aatcgtcttt gtaaattcca caagtgataa 5580

taaaaccgtt cattgaatag cgggttctat tggtttctgt ttgcagcgtt tctttcgcgc 5640

gttgaagtaa tgtctctgct tcaggcaaat tgttaatatg ttcgctgtaa atctgccagc 5700

ccatggattt agttaaatca taatcacttt caatccatgt tttggcgaaa aggagtgcat 5760

catcacgttt gcttacaatc gatgctagtc caaaagttag ttgtgaccat gtttcgcgaa 5820

aagcgatatt ccatttttcg atttgttcgg tggttatttt gtttggatta attgctagca 5880

aaccgagata aatcaagtca ctattatttg attcaatcag ttttacggcg agttcgtgat 5940

tttttgtcag tttttctcgg cggatgattt tctttaaatc accaattttt agtccgtaaa 6000

gatctaatga atccggacaa ccgtgattac gaaaaatttt gatcgtattg gggttttcta 6060

aggcttgtag ctcggtgtca agttggtcaa aagtaatcat acgcgtcact cctctcgaat 6120

aaagtaagta taacaaaaaa agcatgcgaa ggcgcatgct ttaggattta agaatattag 6180

tctatttgtt tcattgcgtc gttctagaac ttttttaagt gtatctgcgg agtttttcat 6240

ttgttctttt tctttgtcat ttaagttcat ttcaacaata tggcgaacgc cttgacggtt 6300

aacgactgct ggtgcaccta tataaatatc gttcataccg taatggccat ctaaataaac 6360

agaaagtggc aaaatcgcat tttcgttatt tagaattgct tttgtaatac gagcaagagc 6420

tgcagcaacg ccgtagaatg tagcgccttt tttattaata atttcataag ctgcatcacg 6480

aacacttacg aaaatagtat ccattgcacc ttgttcatct tcgctaatcc attcagtaat 6540

tggaaggccg ccgacagttg tgtggctcca tgctgggaat tcttgaagac gaaagggcct 6600

cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg 6660

tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 6720

aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 6780

gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 6840

ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 6900

gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 6960

tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 7020

attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 7080

tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 7140

agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 7200

aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 7260

tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 7320

cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 7380

tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 7440

tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 7500

tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 7560

tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 7620

aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 7680

gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 7740

tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 7800

aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 7860

aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 7920

tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 7980

gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 8040

cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 8100

acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 8160

cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 8220

cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 8280

aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 8340

gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 8400

atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 8460

tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 8520

gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 8580

agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg 8640

catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact 8700

ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac 8760

gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc 8820

gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg 8880

taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc 8940

agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta 9000

agggcggttt tttcctgttt ggtcacttga tgcctccgtg taagggggaa tttctgttca 9060

tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg 9120

aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg 9180

accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc 9240

cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg 9300

acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc 9360

aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat 9420

tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga 9480

tcatgcgcac ccgtggccag gacccaacgc tgcccgacga tgataagctg tcaaacatga 9540

<210> 4

<211> 7967

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 4

gaattcccga ttatgtcttt tgcgcactcg gcttaaacca gttttcgctg gtgcgaaaaa 60

agagtgtctt gtgacaccta aattcaaaat ctatcggtca gatttatacc gatttgattt 120

tatatattct tgaataacat acgccgagtt atcacataaa agcgggaacc aatcatcaaa 180

tttaaacttc attgcataat ccattaaact cttaaattct acgattcctt gttcatcaat 240

aaactcaatc atttctttaa ttaatttata tctatctgtt gttgttttct ttaataattc 300

atcaacatct acaccgccat aaactatcat atcttctttt tgatatttaa atttattagg 360

atcgtccatg tgaagcatat atctcacaag acctttcaca cttcctgcaa tctgcggaat 420

agtcgcattc aattcttctg ttattatttt tatctgttca taagatttat taccctcata 480

catcactaga atatgataat gctctttttt catcctacct tctgtatcag tatccctatc 540

atgtaatgga gcactacaaa ttgaatgtgt aactctttta aatactctaa ccactcggct 600

ttgctgattc tggatataaa acaaatgtcc aattacgtcc tcttgaattt ttcttgtttt 660

cagtttcttt tattacattt tcgctcatga tataataacg gtgctaatac acttaacaaa 720

atttagtcat agataggcag catgccagtg ctgtctatct ttttttgttt aaaatgcacc 780

gtattcctcc tttgcatatt tttttattag aataccggtt gcatctgatt tgctaatatt 840

atatttttct ttgattctat ttaatatctc attttcttct gttgtaagtc ttaaagtaac 900

agcaactttt ttctcttctt ttctatctac aactatcact gtacctccca acatctgttt 960

ttttcacttt aacataaaaa acaacctttt aacattaaaa acccaatatt tatttatttg 1020

tttggacaat ggacaatgga cacctagggg ggaggtcgta gtacccccct atgttttctc 1080

ccctaaataa ccccaaaaat ctaagaaaaa aagacctcaa aaaggtcttt aattaacatc 1140

tcaaatttcg catttattcc aatttccttt ttgcgtgtga tgcgctgcgt ccattaaaaa 1200

tcctagagct ttgcaaccga aagttaatag ctgtcgctac tactttcgct tacgctctaa 1260

gtatatttta aggactgtca cacgcaaaaa gttttctcgg cataaaagta cctctacatc 1320

tctaaatcgt ctgtacgctg tttctcacgc tttctatcga tcccgcaaga ggcccggcag 1380

taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat 1440

gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa 1500

actaccgcat taaagcttgt cgacgattca caaaaaatag gcacacgaaa aacaagttaa 1560

gggatgcagt ttatgcatcc cttaacttac ttattaaata atttatagct attgacaaga 1620

gataagaatt gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa 1680

ttgttaaatt gattttttgt aaatattttc ttgtattctt tgttaaccca tttcagaacg 1740

aaataattat acttttgttt atctttgtgt gatattcttg atttttttct acttaatctg 1800

ataagtgagc tattcacttt aggtttagga tgaaaatatt ctcttggaac catacttaat 1860

atagaaatat caacttctgc cattaaaagt aatgccaatg agcgttttgt atttaataat 1920

cttttagcaa acccgtattc cacgattaaa taaatctcat tagctatact atcaagaaca 1980

attttgcgta ttatatccgt acttatgtta taaggtatat taccatatat tttataggat 2040

tggtttttag gaaatttaaa ctgcaatata tccttgttta aaacttggaa attatcgtga 2100

tcaacaagtt tattttctgt agttttgcat aatttatggt ctatttcaat ggcagttacg 2160

aaattacacc tctttactaa ttcaagggta aaatggcctt ttcctgagcc gatttcaaag 2220

atattatcat gttcatttaa tcttatattt gtcattattt tatctatatt atgttttgaa 2280

gtaataaagt tttgactgtg ttttatattt ttctcgttca ttataaccct ctttaatttg 2340

gttatatgaa ttttgcttat taacgattca ttataaccac ttattttttg tttggttgat 2400

aatgaactgt gctgattaca aaaatactaa aaatgcccat attttttcct ccttataaaa 2460

ttagtataat tatagcacga gctctgataa atatgaacat gatgagtgat cgttaaattt 2520

atactgcaat cggatgcgat tattgaataa aagatatgag agatttatct aatttctttt 2580

ttcttgtaaa aaaagaaagt tcttaaaggt tttatagttt tggtcgtaga gcacacggtt 2640

taacgactta attacgaagt aaataagtct agtgtgttag actttatgaa atctttatac 2700

gtttatatat atttattatc cggaggtgta gcatgtctca ttcaattttg agggttgcca 2760

gagttaaagg atcaagtaat acaaacggga tacaaagaca taatcaaaga gagaataaaa 2820

actataataa taaagacata aatcatgagg aaacatataa aaattatgat ttgattaacg 2880

cacaaaatat aaagtataaa gataaaattg atgaaacgat tgatgagaat tattcaggga 2940

aacgtaaaat tcggtcagat gcaattcgcc ccgggactag ttaaaggtgg agaaattgat 3000

tcgtttgtcc attatggctt gaattgcaat aatgcctttt gggatggcca agaaattctt 3060

tatggagatg gggacaaaaa gaatttcaaa ccattttcat gcgccaaaac tattgttggt 3120

catgaactaa cgcatgcagt tatccagtat tcggcgggat tggaatacga agggcaatca 3180

ggtgcgctaa acgagtcgtt cgccgatgtt tttggttatt ttattgcgcc aaatcattgg 3240

ttgattggtg aggatgtctg tgtgcgtggg tcgcgagatg ggcgaataag aagcattaaa 3300

gatcctgaca aatataatca agcggctcat atgaaggatt acgaatcgct tccaatcaca 3360

gaggaaggcg actggggcgg agttcattat aatagtggta tcccgaataa agcagcctat 3420

aatactatca ctaaacttgg aaaagaaaaa acagaacagc gacgatttcg cgccttaaag 3480

tactatttaa cgaaaaaagc ccagtttacc gatgcgaaaa aagcgcttca acaagcagcg 3540

aaagatttat atggtgaaga tgcttctaaa aaagttgctg aagcttggga agcggtagga 3600

gttaactgag cggccgcaca aaaaacggaa atcagttagt aaaactggtt tccgtttttt 3660

attaatagtc ttgagcctca ataacaacag cagtgccaga tgctgtaacc attaacatac 3720

cattatccgc tccaagtact tcataatcga tatccacacc aattacggca tttgctccga 3780

tatctttggc gcgctgttcc atctctttaa tagcttcctc acgtgcgtta attaactcgt 3840

cttcataacc ttgtgatcgt ccaccgaaaa agtttctgag gccagctcca atgtctttca 3900

taaaattaac accagtaatt acttctccga aaacgatttt tttatattcg ataatttgct 3960

tgccttcaat atttggtgag gtagttacaa tcattattaa atccctccag ttttctttta 4020

tcatacctct aagttgtttt tttagtcaaa ggatagctgg ttatttttta cgggcgtggg 4080

ctttttcgat ataagtaatc gcatctggga ctttgcaagc agtttttcct aaatctacat 4140

atactttacc tattgattct gctgcttcca atgcttttgt gtaaagttcc tctttataaa 4200

ttccgcaagc gatgatataa ctattcatag agtaacgagt tctgttcgtt tcagattgta 4260

aagattcttt tgcgagttcc agtaattcct cggcttcagg caaagtcgcg atgtgttctg 4320

tgaaaatttg ccagcccatt gatttagtta aatcagcatc acttttaatc catttcctag 4380

caaaggtgag tgcgtcatca cgtttactta caacggaggc tagcccaaaa gttaacatcg 4440

tccaagcatc tcgaaaagct atattccatt tctcgatttg tgaaacagct acttttttag 4500

ggttcacggc aagtaaacca aggtaaatta agtcgctatt attagactcg attaattgga 4560

gtgctaattc gtgattttta ttcaattttt cacggcgaat aattttcttt aaatcaccaa 4620

tctttaatct ctagaacttt tttaagtgta tctgcggagt ttttcatttg ttctttttct 4680

ttgtcattta agttcatttc aacaatatgg cgaacgcctt gacggttaac gactgctggt 4740

gcacctatat aaatatcgtt cataccgtaa tggccatcta aataaacaga aagtggcaaa 4800

atcgcatttt cgttatttag aattgctttt gtaatacgag caagagctgc agcaacgccg 4860

tagaatgtag cgcctttttt attaataatt tcataagctg catcacgaac acttacgaaa 4920

atagtatcca ttgcaccttg ttcatcttcg ctaatccatt cagtaattgg aaggccgccg 4980

acagttgtgt ggctccatgc tgggaattct tgaagacgaa agggcctcgt gatacgccta 5040

tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 5100

ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 5160

ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 5220

attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 5280

gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 5340

ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 5400

cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt 5460

gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 5520

tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 5580

gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 5640

ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 5700

tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca 5760

gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 5820

caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 5880

cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 5940

atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 6000

gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 6060

attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6120

cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6180

atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 6240

tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 6300

ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 6360

ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 6420

cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 6480

gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 6540

gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 6600

acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 6660

gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6720

agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 6780

tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 6840

agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 6900

cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 6960

gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 7020

ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact 7080

ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac 7140

gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 7200

cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 7260

gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag 7320

cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt 7380

tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt 7440

cctgtttggt cacttgatgc ctccgtgtaa gggggaattt ctgttcatgg gggtaatgat 7500

accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt 7560

actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat 7620

cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca 7680

gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc 7740

cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt 7800

tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt 7860

aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg 7920

tggccaggac ccaacgctgc ccgacgatga taagctgtca aacatga 7967

<210> 5

<211> 8130

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 5

gaattcccga ttatgtcttt tgcgcactcg gcttaaacca gttttcgctg gtgcgaaaaa 60

agagtgtctt gtgacaccta aattcaaaat ctatcggtca gatttatacc gatttgattt 120

tatatattct tgaataacat acgccgagtt atcacataaa agcgggaacc aatcatcaaa 180

tttaaacttc attgcataat ccattaaact cttaaattct acgattcctt gttcatcaat 240

aaactcaatc atttctttaa ttaatttata tctatctgtt gttgttttct ttaataattc 300

atcaacatct acaccgccat aaactatcat atcttctttt tgatatttaa atttattagg 360

atcgtccatg tgaagcatat atctcacaag acctttcaca cttcctgcaa tctgcggaat 420

agtcgcattc aattcttctg ttattatttt tatctgttca taagatttat taccctcata 480

catcactaga atatgataat gctctttttt catcctacct tctgtatcag tatccctatc 540

atgtaatgga gcactacaaa ttgaatgtgt aactctttta aatactctaa ccactcggct 600

ttgctgattc tggatataaa acaaatgtcc aattacgtcc tcttgaattt ttcttgtttt 660

cagtttcttt tattacattt tcgctcatga tataataacg gtgctaatac acttaacaaa 720

atttagtcat agataggcag catgccagtg ctgtctatct ttttttgttt aaaatgcacc 780

gtattcctcc tttgcatatt tttttattag aataccggtt gcatctgatt tgctaatatt 840

atatttttct ttgattctat ttaatatctc attttcttct gttgtaagtc ttaaagtaac 900

agcaactttt ttctcttctt ttctatctac aactatcact gtacctccca acatctgttt 960

ttttcacttt aacataaaaa acaacctttt aacattaaaa acccaatatt tatttatttg 1020

tttggacaat ggacaatgga cacctagggg ggaggtcgta gtacccccct atgttttctc 1080

ccctaaataa ccccaaaaat ctaagaaaaa aagacctcaa aaaggtcttt aattaacatc 1140

tcaaatttcg catttattcc aatttccttt ttgcgtgtga tgcgctgcgt ccattaaaaa 1200

tcctagagct ttgcaaccga aagttaatag ctgtcgctac tactttcgct tacgctctaa 1260

gtatatttta aggactgtca cacgcaaaaa gttttctcgg cataaaagta cctctacatc 1320

tctaaatcgt ctgtacgctg tttctcacgc tttctatcga tcccgcaaga ggcccggcag 1380

taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat 1440

gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa 1500

actaccgcat taaagcttgt cgacgattca caaaaaatag gcacacgaaa aacaagttaa 1560

gggatgcagt ttatgcatcc cttaacttac ttattaaata atttatagct attgacaaga 1620

gataagaatt gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa 1680

ttgttaaatt gattttttgt aaatattttc ttgtattctt tgttaaccca tttcagaacg 1740

aaataattat acttttgttt atctttgtgt gatattcttg atttttttct acttaatctg 1800

ataagtgagc tattcacttt aggtttagga tgaaaatatt ctcttggaac catacttaat 1860

atagaaatat caacttctgc cattaaaagt aatgccaatg agcgttttgt atttaataat 1920

cttttagcaa acccgtattc cacgattaaa taaatctcat tagctatact atcaagaaca 1980

attttgcgta ttatatccgt acttatgtta taaggtatat taccatatat tttataggat 2040

tggtttttag gaaatttaaa ctgcaatata tccttgttta aaacttggaa attatcgtga 2100

tcaacaagtt tattttctgt agttttgcat aatttatggt ctatttcaat ggcagttacg 2160

aaattacacc tctttactaa ttcaagggta aaatggcctt ttcctgagcc gatttcaaag 2220

atattatcat gttcatttaa tcttatattt gtcattattt tatctatatt atgttttgaa 2280

gtaataaagt tttgactgtg ttttatattt ttctcgttca ttataaccct ctttaatttg 2340

gttatatgaa ttttgcttat taacgattca ttataaccac ttattttttg tttggttgat 2400

aatgaactgt gctgattaca aaaatactaa aaatgcccat attttttcct ccttataaaa 2460

ttagtataat tatagcacga gctctgataa atatgaacat gatgagtgat cgttaaattt 2520

atactgcaat cggatgcgat tattgaataa aagatatgag agatttatct aatttctttt 2580

ttcttgtaaa aaaagaaagt tcttaaaggt tttatagttt tggtcgtaga gcacacggtt 2640

taacgactta attacgaagt aaataagtct agtgtgttag actttatgaa atctttatac 2700

gtttatatat atttattatc cggaggtgta gcatgtctca ttcaattttg agggttgcca 2760

gagttaaagg atcaagtaat acaaacggga tacaaagaca taatcaaaga gagaataaaa 2820

actataataa taaagacata aatcatgagg aaacatataa aaattatgat ttgattaacg 2880

cacaaaatat aaagtataaa gataaaattg atgaaacgat tgatgagaat tattcaggga 2940

aacgtaaaat tcggtcagat gcaattcgcc ccgggactag ttaaaggtgg agaaattgat 3000

tcgtttgtcc attatggctt gaattgcaat aatgcctttt gggatggcca agaaattctt 3060

tatggagatg gggacaaaaa gaatttcaaa ccattttcat gcgccaaaac tattgttggt 3120

catgaactaa cgcatgcagt tatccagtat tcggcgggat tggaatacga agggcaatca 3180

ggtgcgctaa acgagtcgtt cgccgatgtt tttggttatt ttattgcgcc aaatcattgg 3240

ttgattggtg aggatgtctg tgtgcgtggg tcgcgagatg ggcgaataag aagcattaaa 3300

gatcctgaca aatataatca agcggctcat atgaaggatt acgaatcgct tccaatcaca 3360

gaggaaggcg actggggcgg agttcattat aatagtggta tcccgaataa agcagcctat 3420

aatactatca ctaaacttgg aaaagaaaaa acagaacagc gacgatttcg cgccttaaag 3480

tactatttaa cgaaaaaagc ccagtttacc gatgcgaaaa aagcgcttca acaagcagcg 3540

aaagatttat atggtgaaga tgcttctaaa aaagttgctg aagcttggga agcggtagga 3600

gttaactgag cggccgctaa taatcttgcg cttcgatgac aacagctgta ccagatgcag 3660

tgaccattag cattccgtta tcagctccaa gcacttcata atcaatatca acaccgataa 3720

cggcatttgc gccaatatct ttcgcacgtt gttccatttc acgaattgct tcctcgcgag 3780

cattaataag ttcatcttca tagccttgcg aacggccccc gaagaaattt cgaagtccag 3840

ccccaatatc tttcataaag ttaacgccag tgatgacttc gccgaaaacg atttttttat 3900

attcgataat ttgtttgcct tcaatatttg gtgaagttgt tacaatcatg agttatccct 3960

acagtttttc ttttatcata cctcttagta ctttttctag tcaaaggata tccggttatt 4020

tcgtacgatt tcgcgctttt tctatataag aaatagcatc tggaacttta caagctgtat 4080

ttccaaggtt tacatgaact ttcccgactg atttcgcggc ttccatcgct ttttcgtgca 4140

aatcgtcttt gtaaattcca caagtgataa taaaaccgtt cattgaatag cgggttctat 4200

tggtttctgt ttgcagcgtt tctttcgcgc gttgaagtaa tgtctctgct tcaggcaaat 4260

tgttaatatg ttcgctgtaa atctgccagc ccatggattt agttaaatca taatcacttt 4320

caatccatgt tttggcgaaa aggagtgcat catcacgttt gcttacaatc gatgctagtc 4380

caaaagttag ttgtgaccat gtttcgcgaa aagcgatatt ccatttttcg atttgttcgg 4440

tggttatttt gtttggatta attgctagca aaccgagata aatcaagtca ctattatttg 4500

attcaatcag ttttacggcg agttcgtgat tttttgtcag tttttctcgg cggatgattt 4560

tctttaaatc accaattttt agtccgtaaa gatctaatga atccggacaa ccgtgattac 4620

gaaaaatttt gatcgtattg gggttttcta aggcttgtag ctcggtgtca agttggtcaa 4680

aagtaatcat acgcgtcact cctctcgaat aaagtaagta taacaaaaaa agcatgcgaa 4740

ggcgcatgct ttaggattta agaatattag tctatttgtt tcattgcgtc gttctagaac 4800

ttttttaagt gtatctgcgg agtttttcat ttgttctttt tctttgtcat ttaagttcat 4860

ttcaacaata tggcgaacgc cttgacggtt aacgactgct ggtgcaccta tataaatatc 4920

gttcataccg taatggccat ctaaataaac agaaagtggc aaaatcgcat tttcgttatt 4980

tagaattgct tttgtaatac gagcaagagc tgcagcaacg ccgtagaatg tagcgccttt 5040

tttattaata atttcataag ctgcatcacg aacacttacg aaaatagtat ccattgcacc 5100

ttgttcatct tcgctaatcc attcagtaat tggaaggccg ccgacagttg tgtggctcca 5160

tgctgggaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 5220

catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 5280

ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 5340

ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 5400

cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 5460

ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 5520

tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 5580

cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca 5640

actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 5700

aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 5760

tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 5820

ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 5880

tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt 5940

gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 6000

gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 6060

tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 6120

gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 6180

ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 6240

gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 6300

aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 6360

ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 6420

ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 6480

tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 6540

gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 6600

agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 6660

taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 6720

gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 6780

gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 6840

caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 6900

aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 6960

tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 7020

acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 7080

ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 7140

gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct 7200

ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc 7260

tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct 7320

gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 7380

tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 7440

tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat 7500

tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat 7560

gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcacttga 7620

tgcctccgtg taagggggaa tttctgttca tgggggtaat gataccgatg aaacgagaga 7680

ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg 7740

gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc 7800

agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc 7860

agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac 7920

ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc 7980

ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc 8040

ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc 8100

tgcccgacga tgataagctg tcaaacatga 8130

<210> 6

<211> 12118

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 6

gaattcccga ttatgtcttt tgcgcactcg gcttaaacca gttttcgctg gtgcgaaaaa 60

agagtgtctt gtgacaccta aattcaaaat ctatcggtca gatttatacc gatttgattt 120

tatatattct tgaataacat acgccgagtt atcacataaa agcgggaacc aatcatcaaa 180

tttaaacttc attgcataat ccattaaact cttaaattct acgattcctt gttcatcaat 240

aaactcaatc atttctttaa ttaatttata tctatctgtt gttgttttct ttaataattc 300

atcaacatct acaccgccat aaactatcat atcttctttt tgatatttaa atttattagg 360

atcgtccatg tgaagcatat atctcacaag acctttcaca cttcctgcaa tctgcggaat 420

agtcgcattc aattcttctg ttattatttt tatctgttca taagatttat taccctcata 480

catcactaga atatgataat gctctttttt catcctacct tctgtatcag tatccctatc 540

atgtaatgga gcactacaaa ttgaatgtgt aactctttta aatactctaa ccactcggct 600

ttgctgattc tggatataaa acaaatgtcc aattacgtcc tcttgaattt ttcttgtttt 660

cagtttcttt tattacattt tcgctcatga tataataacg gtgctaatac acttaacaaa 720

atttagtcat agataggcag catgccagtg ctgtctatct ttttttgttt aaaatgcacc 780

gtattcctcc tttgcatatt tttttattag aataccggtt gcatctgatt tgctaatatt 840

atatttttct ttgattctat ttaatatctc attttcttct gttgtaagtc ttaaagtaac 900

agcaactttt ttctcttctt ttctatctac aactatcact gtacctccca acatctgttt 960

ttttcacttt aacataaaaa acaacctttt aacattaaaa acccaatatt tatttatttg 1020

tttggacaat ggacaatgga cacctagggg ggaggtcgta gtacccccct atgttttctc 1080

ccctaaataa ccccaaaaat ctaagaaaaa aagacctcaa aaaggtcttt aattaacatc 1140

tcaaatttcg catttattcc aatttccttt ttgcgtgtga tgcgctgcgt ccattaaaaa 1200

tcctagagct ttgcaaccga aagttaatag ctgtcgctac tactttcgct tacgctctaa 1260

gtatatttta aggactgtca cacgcaaaaa gttttctcgg cataaaagta cctctacatc 1320

tctaaatcgt ctgtacgctg tttctcacgc tttctatcga tcccgcaaga ggcccggcag 1380

taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat 1440

gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa 1500

actaccgcat taaagcttgt cgacgattca caaaaaatag gcacacgaaa aacaagttaa 1560

gggatgcagt ttatgcatcc cttaacttac ttattaaata atttatagct attgacaaga 1620

gataagaatt gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa 1680

ttgttaaatt gattttttgt aaatattttc ttgtattctt tgttaaccca tttcagaacg 1740

aaataattat acttttgttt atctttgtgt gatattcttg atttttttct acttaatctg 1800

ataagtgagc tattcacttt aggtttagga tgaaaatatt ctcttggaac catacttaat 1860

atagaaatat caacttctgc cattaaaagt aatgccaatg agcgttttgt atttaataat 1920

cttttagcaa acccgtattc cacgattaaa taaatctcat tagctatact atcaagaaca 1980

attttgcgta ttatatccgt acttatgtta taaggtatat taccatatat tttataggat 2040

tggtttttag gaaatttaaa ctgcaatata tccttgttta aaacttggaa attatcgtga 2100

tcaacaagtt tattttctgt agttttgcat aatttatggt ctatttcaat ggcagttacg 2160

aaattacacc tctttactaa ttcaagggta aaatggcctt ttcctgagcc gatttcaaag 2220

atattatcat gttcatttaa tcttatattt gtcattattt tatctatatt atgttttgaa 2280

gtaataaagt tttgactgtg ttttatattt ttctcgttca ttataaccct ctttaatttg 2340

gttatatgaa ttttgcttat taacgattca ttataaccac ttattttttg tttggttgat 2400

aatgaactgt gctgattaca aaaatactaa aaatgcccat attttttcct ccttataaaa 2460

ttagtataat tatagcacga gctctgataa atatgaacat gatgagtgat cgttaaattt 2520

atactgcaat cggatgcgat tattgaataa aagatatgag agatttatct aatttctttt 2580

ttcttgtaaa aaaagaaagt tcttaaaggt tttatagttt tggtcgtaga gcacacggtt 2640

taacgactta attacgaagt aaataagtct agtgtgttag actttatgaa atctttatac 2700

gtttatatat atttattatc cggaggtgta gcatgtctca ttcaattttg agggttgcca 2760

gagttaaagg atcaagtaat acaaacggga tacaaagaca taatcaaaga gagaataaaa 2820

actataataa taaagacata aatcatgagg aaacatataa aaattatgat ttgattaacg 2880

cacaaaatat aaagtataaa gataaaattg atgaaacgat tgatgagaat tattcaggga 2940

aacgtaaaat tcggtcagat gcaattcgcc ccgggactag ttaaaggtgg agaaattgat 3000

tcgtttgtcc attatggctt gaattgcaat aatgcctttt gggatggcca agaaattctt 3060

tatggagatg gggacaaaaa gaatttcaaa ccattttcat gcgccaaaac tattgttggt 3120

catgaactaa cgcatgcagt tatccagtat tcggcgggat tggaatacga agggcaatca 3180

ggtgcgctaa acgagtcgtt cgccgatgtt tttggttatt ttattgcgcc aaatcattgg 3240

ttgattggtg aggatgtctg tgtgcgtggg tcgcgagatg ggcgaataag aagcattaaa 3300

gatcctgaca aatataatca agcggctcat atgaaggatt acgaatcgct tccaatcaca 3360

gaggaaggcg actggggcgg agttcattat aatagtggta tcccgaataa agcagcctat 3420

aatactatca ctaaacttgg aaaagaaaaa acagaacagc gacgatttcg cgccttaaag 3480

tactatttaa cgaaaaaagc ccagtttacc gatgcgaaaa aagcgcttca acaagcagcg 3540

aaagatttat atggtgaaga tgcttctaaa aaagttgctg aagcttggga agcggtagga 3600

gttaactgag cggccgctag ctttaaggct aaatgccgaa tggttggcac ctaccgcatt 3660

ggcaaccgtg gcagaagagg gcgcatccgt tttggcgaaa aagagtaaaa cggcgaggat 3720

gagtgcacag ccagagccca gccagaaaac aaactgatta ttgatggtga acatgatgcc 3780

gacaatcgag gcacacagcg cccagccaac acagccaaac atccgcgcgc gaccaaattc 3840

gaaattactg cgacggctga ctttctcaat aaatgcctct actgctggcg caccggcgtt 3900

aaaacaaaag cctagataaa taccaccaac aatcgatcct actaaaatgt tgtattgtaa 3960

cagtggcccg aagataaaaa taaagaacgg cgcaaacatc actaacatgc cggtaataat 4020

ccacagcagg tatttgcgca gcccgagttt gtcagaaagc agaccaaaca gcggttggaa 4080

taatagcgag aacagagaaa tagcggcaaa aataataccc gtatcacttt tgctgatatg 4140

gttgatgtca tgtagccaaa tcgggaaaaa cgggaagtag gctcccatga taaaaaagta 4200

aaagaaaaag aataaaccga acatccaaaa gtttgtgttt tttaaatagt acataatgga 4260

tttccttacg cgaaatacgg gcagacatgg cctgcccggt tattattatt tttgacacca 4320

gaccaactgg taatggtagc gaccggcgct cagctggaat taattccgcc gatactgacg 4380

ggctccagga gtcgtcgcca ccaatcccca tatggaaacc gtcgatattc agccatgtgc 4440

cttcttccgc gtgcagcaga tggcgatggc tggtttccat cagttgctgt tgactgtagc 4500

ggctgatgtt gaactggaag tcgccgcgcc actggtgtgg gccataattc aattcgcgcg 4560

tcccgcagcg cagaccgttt tcgctcggga agacgtacgg ggtatacatg tctgacaatg 4620

gcagatccca gcggtcaaaa caggcggcag taaggcggtc gggatagttt tcttgcggcc 4680

ctaatccgag ccagtttacc cgctctgcta cctgcgccag ctggcagttc aggccaatcc 4740

gcgccggatg cggtgtatcg ctcgccactt caacatcaac ggtaatcgcc atttgaccac 4800

taccatcaat ccggtaggtt ttccggctga taaataaggt tttcccctga tgctgccacg 4860

cgtgagcggt cgtaatcagc accgcatcag caagtgtatc tgccgtgcac tgcaacaacg 4920

ctgcttcggc ctggtaatgg cccgccgcct tccagcgttc gacccaggcg ttagggtcaa 4980

tgcgggtcgc ttcacttacg ccaatgtcgt tatccagcgg tgcacgggtg aactgatcgc 5040

gcagcggcgt cagcagttgt tttttatcgc caatccacat ctgtgaaaga aagcctgact 5100

ggcggttaaa ttgccaacgc ttattaccca gctcgatgca aaaatccatt tcgctggtgg 5160

tcagatgcgg gatggcgtgg gacgcggcgg ggagcgtcac actgaggttt tccgccagac 5220

gccactgctg ccaggcgctg atgtgcccgg cttctgacca tgcggtcgcg ttcggttgca 5280

ctacgcgtac tgtgagccag agttgcccgg cgctctccgg ctgcggtagt tcaggcagtt 5340

caatcaactg tttaccttgt ggagcgacat ccagaggcac ttcaccgctt gccagcggct 5400

taccatccag cgccaccatc cagtgcagga gctcgttatc gctatgacgg aacaggtatt 5460

cgctggtcac ttcgatggtt tgcccggata aacggaactg gaaaaactgc tgctggtgtt 5520

ttgcttccgt cagcgctgga tgcggcgtgc ggtcggcaaa gaccagaccg ttcatacaga 5580

actggcgatc gttcggcgta tcgccaaaat caccgccgta agccgaccac gggttgccgt 5640

tttcatcata tttaatcagc gactgatcca cccagtccca gacgaagccg ccctgtaaac 5700

ggggatactg acgaaacgcc tgccagtatt tagcgaaacc gccaagactg ttacccatcg 5760

cgtgggcgta ttcgcaaagg atcagcgggc gcgtctctcc aggtagcgaa agccattttt 5820

tgatggacca tttcggcaca gccgggaagg gctggtcttc atccacgcgc gcgtacatcg 5880

ggcaaataat atcggtggcc gtggtgtcgg ctccgccgcc ttcatactgc accgggcggg 5940

aaggatcgac agatttgatc cagcgataca gcgcgtcgtg attagcgccg tggcctgatt 6000

cattccccag cgaccagatg atcacactcg ggtgattacg atcgcgctgc accattcgcg 6060

ttacgcgttc gctcatcgcc ggtagccagc gcggatcatc ggtcagacga ttcattggca 6120

ccatgccgtg ggtttcaata ttggcttcat ccaccacata caggccgtag cggtcgcaca 6180

gcgtgtacca cagcggatgg ttcggataat gcgaacagcg cacggcgtta aagttgttct 6240

gcttcatcag caggatatcc tgcaccatcg tctgctcatc catgacctga ccatgcagag 6300

gatgatgctc gtgacggtta acgcctcgaa tcagcaacgg cttgccgttc agcagcagca 6360

gaccattttc aatccgcacc tcgcggaaac cgacatcgca ggcttctgct tcaatcagcg 6420

tgccgtcggc ggtgtgcagt tcaaccaccg cacgatagag attcgggatt tcggcgctcc 6480

acagtttcgg gttttcgacg ttcagacgta gtgtgacgcg atcggcataa ccaccacgct 6540

catcgataat ttcaccgccg aaaggcgcgg tgccgctggc gacctgcgtt tcaccctgcc 6600

ataaagaaac tgttacccgt aggtagtcac gcaactcgcc gcacatctga acttcagcct 6660

ccagtacagc gcggctgaaa tcatcattaa agcgagtggc aacatggaaa tcgctgattt 6720

gtgtagtcgg tttatgcagc aacgagacgt cacggaaaat gccgctcatc cgccacatat 6780

cctgatcttc cagataactg ccgtcactcc aacgcagcac catcaccgcg aggcggtttt 6840

ctccggcgcg taaaaatgcg ctcaggtcaa attcagacgg caaacgactg tcctggccgt 6900

aaccgaccca gcgcccgttg caccacagat gaaacgccga gttaacgcca tcaaaaataa 6960

ttcgcgtctg gccttcctgt agccagcttt catcaacatt aaatgtgagc gagtaacaac 7020

ccgtcggatt ctccgtggga acaaacggcg gattgaccgt aatgggatag gttacgttgg 7080

tgtagatggg cgcatcgtaa ccgtgcatct gccagtttga ggggacgacg acagtatcgg 7140

cctcaggaag atcgcactcc agccagcttt ccggcaccgc ttctggtgcc ggaaaccagg 7200

caaagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 7260

cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 7320

cgccagggtt ttcccagtca cgacgttgta aaacgacggg atccataaaa actagcatta 7380

tttttttcat gggtttcact ctccttctac attttttaac ctaataatgc caaataccgt 7440

ttgccacccc tctcttttga taattataat attggcgaaa ttcgcttcta aagatgaaac 7500

gcaatattat atgcttgctt tatagcttta ttctagtcct gctgtccctt tatcgtcgtt 7560

aacaaatgtt aatgcctcaa cataaaagtc actctaggcg gccgctaata atcttgcgct 7620

tcgatgacaa cagctgtacc agatgcagtg accattagca ttccgttatc agctccaagc 7680

acttcataat caatatcaac accgataacg gcatttgcgc caatatcttt cgcacgttgt 7740

tccatttcac gaattgcttc ctcgcgagca ttaataagtt catcttcata gccttgcgaa 7800

cggcccccga agaaatttcg aagtccagcc ccaatatctt tcataaagtt aacgccagtg 7860

atgacttcgc cgaaaacgat ttttttatat tcgataattt gtttgccttc aatatttggt 7920

gaagttgtta caatcatgag ttatccctac agtttttctt ttatcatacc tcttagtact 7980

ttttctagtc aaaggatatc cggttatttc gtacgatttc gcgctttttc tatataagaa 8040

atagcatctg gaactttaca agctgtattt ccaaggttta catgaacttt cccgactgat 8100

ttcgcggctt ccatcgcttt ttcgtgcaaa tcgtctttgt aaattccaca agtgataata 8160

aaaccgttca ttgaatagcg ggttctattg gtttctgttt gcagcgtttc tttcgcgcgt 8220

tgaagtaatg tctctgcttc aggcaaattg ttaatatgtt cgctgtaaat ctgccagccc 8280

atggatttag ttaaatcata atcactttca atccatgttt tggcgaaaag gagtgcatca 8340

tcacgtttgc ttacaatcga tgctagtcca aaagttagtt gtgaccatgt ttcgcgaaaa 8400

gcgatattcc atttttcgat ttgttcggtg gttattttgt ttggattaat tgctagcaaa 8460

ccgagataaa tcaagtcact attatttgat tcaatcagtt ttacggcgag ttcgtgattt 8520

tttgtcagtt tttctcggcg gatgattttc tttaaatcac caatttttag tccgtaaaga 8580

tctaatgaat ccggacaacc gtgattacga aaaattttga tcgtattggg gttttctaag 8640

gcttgtagct cggtgtcaag ttggtcaaaa gtaatcatac gcgtcactcc tctcgaataa 8700

agtaagtata acaaaaaaag catgcgaagg cgcatgcttt aggatttaag aatattagtc 8760

tatttgtttc attgcgtcgt tctagaactt ttttaagtgt atctgcggag tttttcattt 8820

gttctttttc tttgtcattt aagttcattt caacaatatg gcgaacgcct tgacggttaa 8880

cgactgctgg tgcacctata taaatatcgt tcataccgta atggccatct aaataaacag 8940

aaagtggcaa aatcgcattt tcgttattta gaattgcttt tgtaatacga gcaagagctg 9000

cagcaacgcc gtagaatgta gcgccttttt tattaataat ttcataagct gcatcacgaa 9060

cacttacgaa aatagtatcc attgcacctt gttcatcttc gctaatccat tcagtaattg 9120

gaaggccgcc gacagttgtg tggctccatg ctgggaattc ttgaagacga aagggcctcg 9180

tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 9240

gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 9300

atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 9360

agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 9420

ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 9480

gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 9540

gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 9600

tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 9660

acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 9720

aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 9780

cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 9840

gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 9900

cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 9960

tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 10020

tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 10080

ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 10140

tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 10200

gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 10260

ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 10320

tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 10380

agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 10440

aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 10500

cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 10560

agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 10620

tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 10680

gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 10740

gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 10800

ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 10860

gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 10920

ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 10980

ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 11040

acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 11100

gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 11160

cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 11220

tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 11280

gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 11340

gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 11400

gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta 11460

aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag 11520

ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag 11580

ggcggttttt tcctgtttgg tcacttgatg cctccgtgta agggggaatt tctgttcatg 11640

ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa 11700

catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac 11760

cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca 11820

cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac 11880

ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag 11940

gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc 12000

tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc 12060

atgcgcaccc gtggccagga cccaacgctg cccgacgatg ataagctgtc aaacatga 12118

<210> 7

<211> 21

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 7

catcaaaaac gtacagcaat g 21

<210> 8

<211> 21

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 8

tggtttttga gaacaaattg g 21

<210> 9

<211> 27

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 9

gataagtcga cgattcacaa aaaatag 27

<210> 10

<211> 23

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 10

aaaactagtc ccggggcgaa ttg 23

<210> 11

<211> 23

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 11

gctataaatg aagaggcttc agg 23

<210> 12

<211> 22

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 12

ctcttaaatc agctaggcga tc 22

<210> 13

<211> 9285

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 13

gaattcccga ttatgtcttt tgcgcactcg gcttaaacca gttttcgctg gtgcgaaaaa 60

agagtgtctt gtgacaccta aattcaaaat ctatcggtca gatttatacc gatttgattt 120

tatatattct tgaataacat acgccgagtt atcacataaa agcgggaacc aatcatcaaa 180

tttaaacttc attgcataat ccattaaact cttaaattct acgattcctt gttcatcaat 240

aaactcaatc atttctttaa ttaatttata tctatctgtt gttgttttct ttaataattc 300

atcaacatct acaccgccat aaactatcat atcttctttt tgatatttaa atttattagg 360

atcgtccatg tgaagcatat atctcacaag acctttcaca cttcctgcaa tctgcggaat 420

agtcgcattc aattcttctg ttattatttt tatctgttca taagatttat taccctcata 480

catcactaga atatgataat gctctttttt catcctacct tctgtatcag tatccctatc 540

atgtaatgga gcactacaaa ttgaatgtgt aactctttta aatactctaa ccactcggct 600

ttgctgattc tggatataaa acaaatgtcc aattacgtcc tcttgaattt ttcttgtttt 660

cagtttcttt tattacattt tcgctcatga tataataacg gtgctaatac acttaacaaa 720

atttagtcat agataggcag catgccagtg ctgtctatct ttttttgttt aaaatgcacc 780

gtattcctcc tttgcatatt tttttattag aataccggtt gcatctgatt tgctaatatt 840

atatttttct ttgattctat ttaatatctc attttcttct gttgtaagtc ttaaagtaac 900

agcaactttt ttctcttctt ttctatctac aactatcact gtacctccca acatctgttt 960

ttttcacttt aacataaaaa acaacctttt aacattaaaa acccaatatt tatttatttg 1020

tttggacaat ggacaatgga cacctagggg ggaggtcgta gtacccccct atgttttctc 1080

ccctaaataa ccccaaaaat ctaagaaaaa aagacctcaa aaaggtcttt aattaacatc 1140

tcaaatttcg catttattcc aatttccttt ttgcgtgtga tgcgctgcgt ccattaaaaa 1200

tcctagagct ttgcaaccga aagttaatag ctgtcgctac tactttcgct tacgctctaa 1260

gtatatttta aggactgtca cacgcaaaaa gttttctcgg cataaaagta cctctacatc 1320

tctaaatcgt ctgtacgctg tttctcacgc tttctatcga tcccgcaaga ggcccggcag 1380

taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat 1440

gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa 1500

actaccgcat taaagcttgt cgacgattca caaaaaatag gcacacgaaa aacaagttaa 1560

gggatgcagt ttatgcatcc cttaacttac ttattaaata atttatagct attgacaaga 1620

gataagaatt gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa 1680

ttgttaaatt gattttttgt aaatattttc ttgtattctt tgttaaccca tttcagaacg 1740

aaataattat acttttgttt atctttgtgt gatattcttg atttttttct acttaatctg 1800

ataagtgagc tattcacttt aggtttagga tgaaaatatt ctcttggaac catacttaat 1860

atagaaatat caacttctgc cattaaaagt aatgccaatg agcgttttgt atttaataat 1920

cttttagcaa acccgtattc cacgattaaa taaatctcat tagctatact atcaagaaca 1980

attttgcgta ttatatccgt acttatgtta taaggtatat taccatatat tttataggat 2040

tggtttttag gaaatttaaa ctgcaatata tccttgttta aaacttggaa attatcgtga 2100

tcaacaagtt tattttctgt agttttgcat aatttatggt ctatttcaat ggcagttacg 2160

aaattacacc tctttactaa ttcaagggta aaatggcctt ttcctgagcc gatttcaaag 2220

atattatcat gttcatttaa tcttatattt gtcattattt tatctatatt atgttttgaa 2280

gtaataaagt tttgactgtg ttttatattt ttctcgttca ttataaccct ctttaatttg 2340

gttatatgaa ttttgcttat taacgattca ttataaccac ttattttttg tttggttgat 2400

aatgaactgt gctgattaca aaaatactaa aaatgcccat attttttcct ccttataaaa 2460

ttagtataat tatagcacga gctctgataa atatgaacat gatgagtgat cgttaaattt 2520

atactgcaat cggatgcgat tattgaataa aagatatgag agatttatct aatttctttt 2580

ttcttgtaaa aaaagaaagt tcttaaaggt tttatagttt tggtcgtaga gcacacggtt 2640

taacgactta attacgaagt aaataagtct agtgtgttag actttatgaa atctttatac 2700

gtttatatat atttattatc cggaggtgta gcatgtctca ttcaattttg agggttgcca 2760

gagttaaagg atcaagtaat acaaacggga tacaaagaca taatcaaaga gagaataaaa 2820

actataataa taaagacata aatcatgagg aaacatataa aaattatgat ttgattaacg 2880

cacaaaatat aaagtataaa gataaaattg atgaaacgat tgatgagaat tattcaggga 2940

aacgtaaaat tcggtcagat gcaattcgcc ccgggactag ttaaaggtgg agaaattgat 3000

tcgtttgtcc attatggctt gaattgcaat aatgcctttt gggatggcca agaaattctt 3060

tatggagatg gggacaaaaa gaatttcaaa ccattttcat gcgccaaaac tattgttggt 3120

catgaactaa cgcatgcagt tatccagtat tcggcgggat tggaatacga agggcaatca 3180

ggtgcgctaa acgagtcgtt cgccgatgtt tttggttatt ttattgcgcc aaatcattgg 3240

ttgattggtg aggatgtctg tgtgcgtggg tcgcgagatg ggcgaataag aagcattaaa 3300

gatcctgaca aatataatca agcggctcat atgaaggatt acgaatcgct tccaatcaca 3360

gaggaaggcg actggggcgg agttcattat aatagtggta tcccgaataa agcagcctat 3420

aatactatca ctaaacttgg aaaagaaaaa acagaacagc gacgatttcg cgccttaaag 3480

tactatttaa cgaaaaaagc ccagtttacc gatgcgaaaa aagcgcttca acaagcagcg 3540

aaagatttat atggtgaaga tgcttctaaa aaagttgctg aagcttggga agcggtagga 3600

gttaactgag cggccgccag tgtgatggat atctgcagaa ttaattcggc tttctagagt 3660

gacttttatg ttgaggcatt aacatttgtt aacgacgata aagggacagc aggactagaa 3720

taaagctata aagcaagcat ataatattgc gtttcatctt tagaagcgaa tttcgccaat 3780

attataatta tcaaaagaga ggggtggcaa acggtatttg gcattattag gttaaaaaat 3840

gtagaaggag agtgaaaccc atgaaaaaaa taatgctagt ttttattaca cttatattag 3900

ttagtctacc aattgcgcaa caaactgaag caaaggatgc atcggatctt aaagctgttt 3960

ataattttgc tactatgaag gatccatatc catatgatgt tccagattat gcatcattga 4020

attcaatgag aagtgaaaga ccacaagctt tgttggggaa gtgcttgacc gcgtgctgtt 4080

gctcgcgatt gctttttttg tggtgtatcg tgccgttcta tcttgctgtg ctcgtcaacg 4140

ccagcaacaa caacagctct catattcagt tgatttataa cttaacgcta tgtgagctga 4200

atggcacaga ttggctggcg caaaaatttg actgggcagt ggagactttt gtcatcttcc 4260

ccgtgttgac tcacattgtt tcctatgggg cactcaccac cagccatttc cttgacacag 4320

ttggtctggc cactgtgtcc accgccggat attatcacgg gcggtatgtc ttgagtagca 4380

tttacgcagt ctgtgctctg gctgcgttga tttgctttgt cattaggctt gcgaagaact 4440

gcatgtcctg gcgctactct tgtaccagat ataccaactt ccttctggac actaagggca 4500

gactctatcg ttggcggtcg cccgtcattg tggagaaagg gggtaaggtt gaggtcgaag 4560

gtcacctgat cgacctcaag agagttgtgc ttgatggttc cgcggcaacc cctttaacca 4620

gagtttcagc ggaacaatgg ggtcgtctcc tcgagggatt aaatggacca gatatttata 4680

aaggagttta tcaatttaaa agtgttgaat ttgatgtcga gtatacagat attgaaatga 4740

atagattagg aaaatgataa gtcggcggcc gctaataatc ttgcgcttcg atgacaacag 4800

ctgtaccaga tgcagtgacc attagcattc cgttatcagc tccaagcact tcataatcaa 4860

tatcaacacc gataacggca tttgcgccaa tatctttcgc acgttgttcc atttcacgaa 4920

ttgcttcctc gcgagcatta ataagttcat cttcatagcc ttgcgaacgg cccccgaaga 4980

aatttcgaag tccagcccca atatctttca taaagttaac gccagtgatg acttcgccga 5040

aaacgatttt tttatattcg ataatttgtt tgccttcaat atttggtgaa gttgttacaa 5100

tcatgagtta tccctacagt ttttctttta tcatacctct tagtactttt tctagtcaaa 5160

ggatatccgg ttatttcgta cgatttcgcg ctttttctat ataagaaata gcatctggaa 5220

ctttacaagc tgtatttcca aggtttacat gaactttccc gactgatttc gcggcttcca 5280

tcgctttttc gtgcaaatcg tctttgtaaa ttccacaagt gataataaaa ccgttcattg 5340

aatagcgggt tctattggtt tctgtttgca gcgtttcttt cgcgcgttga agtaatgtct 5400

ctgcttcagg caaattgtta atatgttcgc tgtaaatctg ccagcccatg gatttagtta 5460

aatcataatc actttcaatc catgttttgg cgaaaaggag tgcatcatca cgtttgctta 5520

caatcgatgc tagtccaaaa gttagttgtg accatgtttc gcgaaaagcg atattccatt 5580

tttcgatttg ttcggtggtt attttgtttg gattaattgc tagcaaaccg agataaatca 5640

agtcactatt atttgattca atcagtttta cggcgagttc gtgatttttt gtcagttttt 5700

ctcggcggat gattttcttt aaatcaccaa tttttagtcc gtaaagatct aatgaatccg 5760

gacaaccgtg attacgaaaa attttgatcg tattggggtt ttctaaggct tgtagctcgg 5820

tgtcaagttg gtcaaaagta atcatacgcg tcactcctct cgaataaagt aagtataaca 5880

aaaaaagcat gcgaaggcgc atgctttagg atttaagaat attagtctat ttgtttcatt 5940

gcgtcgttct agaacttttt taagtgtatc tgcggagttt ttcatttgtt ctttttcttt 6000

gtcatttaag ttcatttcaa caatatggcg aacgccttga cggttaacga ctgctggtgc 6060

acctatataa atatcgttca taccgtaatg gccatctaaa taaacagaaa gtggcaaaat 6120

cgcattttcg ttatttagaa ttgcttttgt aatacgagca agagctgcag caacgccgta 6180

gaatgtagcg ccttttttat taataatttc ataagctgca tcacgaacac ttacgaaaat 6240

agtatccatt gcaccttgtt catcttcgct aatccattca gtaattggaa ggccgccgac 6300

agttgtgtgg ctccatgctg ggaattcttg aagacgaaag ggcctcgtga tacgcctatt 6360

tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg 6420

aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 6480

catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 6540

tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 6600

tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 6660

ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 6720

ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga 6780

cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 6840

ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 6900

tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 6960

gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 7020

ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc 7080

aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 7140

acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 7200

tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 7260

cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 7320

gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 7380

taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 7440

tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 7500

cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 7560

ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 7620

accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 7680

cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 7740

cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 7800

tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 7860

taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 7920

gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 7980

agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 8040

ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 8100

acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 8160

caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 8220

tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 8280

tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct 8340

gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct 8400

cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt 8460

gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 8520

tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 8580

cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg 8640

tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc 8700

tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc 8760

tgtttggtca cttgatgcct ccgtgtaagg gggaatttct gttcatgggg gtaatgatac 8820

cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac 8880

tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca 8940

ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc 9000

agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca 9060

gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt 9120

tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa 9180

ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg 9240

gccaggaccc aacgctgccc gacgatgata agctgtcaaa catga 9285

<210> 14

<211> 9525

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 14

gaattcccga ttatgtcttt tgcgcactcg gcttaaacca gttttcgctg gtgcgaaaaa 60

agagtgtctt gtgacaccta aattcaaaat ctatcggtca gatttatacc gatttgattt 120

tatatattct tgaataacat acgccgagtt atcacataaa agcgggaacc aatcatcaaa 180

tttaaacttc attgcataat ccattaaact cttaaattct acgattcctt gttcatcaat 240

aaactcaatc atttctttaa ttaatttata tctatctgtt gttgttttct ttaataattc 300

atcaacatct acaccgccat aaactatcat atcttctttt tgatatttaa atttattagg 360

atcgtccatg tgaagcatat atctcacaag acctttcaca cttcctgcaa tctgcggaat 420

agtcgcattc aattcttctg ttattatttt tatctgttca taagatttat taccctcata 480

catcactaga atatgataat gctctttttt catcctacct tctgtatcag tatccctatc 540

atgtaatgga gcactacaaa ttgaatgtgt aactctttta aatactctaa ccactcggct 600

ttgctgattc tggatataaa acaaatgtcc aattacgtcc tcttgaattt ttcttgtttt 660

cagtttcttt tattacattt tcgctcatga tataataacg gtgctaatac acttaacaaa 720

atttagtcat agataggcag catgccagtg ctgtctatct ttttttgttt aaaatgcacc 780

gtattcctcc tttgcatatt tttttattag aataccggtt gcatctgatt tgctaatatt 840

atatttttct ttgattctat ttaatatctc attttcttct gttgtaagtc ttaaagtaac 900

agcaactttt ttctcttctt ttctatctac aactatcact gtacctccca acatctgttt 960

ttttcacttt aacataaaaa acaacctttt aacattaaaa acccaatatt tatttatttg 1020

tttggacaat ggacaatgga cacctagggg ggaggtcgta gtacccccct atgttttctc 1080

ccctaaataa ccccaaaaat ctaagaaaaa aagacctcaa aaaggtcttt aattaacatc 1140

tcaaatttcg catttattcc aatttccttt ttgcgtgtga tgcgctgcgt ccattaaaaa 1200

tcctagagct ttgcaaccga aagttaatag ctgtcgctac tactttcgct tacgctctaa 1260

gtatatttta aggactgtca cacgcaaaaa gttttctcgg cataaaagta cctctacatc 1320

tctaaatcgt ctgtacgctg tttctcacgc tttctatcga tcccgcaaga ggcccggcag 1380

taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat 1440

gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa 1500

actaccgcat taaagcttgt cgacgattca caaaaaatag gcacacgaaa aacaagttaa 1560

gggatgcagt ttatgcatcc cttaacttac ttattaaata atttatagct attgacaaga 1620

gataagaatt gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa 1680

ttgttaaatt gattttttgt aaatattttc ttgtattctt tgttaaccca tttcagaacg 1740

aaataattat acttttgttt atctttgtgt gatattcttg atttttttct acttaatctg 1800

ataagtgagc tattcacttt aggtttagga tgaaaatatt ctcttggaac catacttaat 1860

atagaaatat caacttctgc cattaaaagt aatgccaatg agcgttttgt atttaataat 1920

cttttagcaa acccgtattc cacgattaaa taaatctcat tagctatact atcaagaaca 1980

attttgcgta ttatatccgt acttatgtta taaggtatat taccatatat tttataggat 2040

tggtttttag gaaatttaaa ctgcaatata tccttgttta aaacttggaa attatcgtga 2100

tcaacaagtt tattttctgt agttttgcat aatttatggt ctatttcaat ggcagttacg 2160

aaattacacc tctttactaa ttcaagggta aaatggcctt ttcctgagcc gatttcaaag 2220

atattatcat gttcatttaa tcttatattt gtcattattt tatctatatt atgttttgaa 2280

gtaataaagt tttgactgtg ttttatattt ttctcgttca ttataaccct ctttaatttg 2340

gttatatgaa ttttgcttat taacgattca ttataaccac ttattttttg tttggttgat 2400

aatgaactgt gctgattaca aaaatactaa aaatgcccat attttttcct ccttataaaa 2460

ttagtataat tatagcacga gctctgataa atatgaacat gatgagtgat cgttaaattt 2520

atactgcaat cggatgcgat tattgaataa aagatatgag agatttatct aatttctttt 2580

ttcttgtaaa aaaagaaagt tcttaaaggt tttatagttt tggtcgtaga gcacacggtt 2640

taacgactta attacgaagt aaataagtct agtgtgttag actttatgaa atctttatac 2700

gtttatatat atttattatc cggaggtgta gcatgtctca ttcaattttg agggttgcca 2760

gagttaaagg atcaagtaat acaaacggga tacaaagaca taatcaaaga gagaataaaa 2820

actataataa taaagacata aatcatgagg aaacatataa aaattatgat ttgattaacg 2880

cacaaaatat aaagtataaa gataaaattg atgaaacgat tgatgagaat tattcaggga 2940

aacgtaaaat tcggtcagat gcaattcgcc ccgggactag tttcgttatg gcttaaattg 3000

caataacgct ttttgggatg gtcaggaaat tctttatgga gatggagata agaagaatta 3060

caaaccattt tcatgtgcaa aaaatatcgt cgctcatgaa ttaacgcatg ctgtcattca 3120

acactcagca ggtttagaat acgaagggca agcaggtgca ttaaatgaat cattcgcgga 3180

tgtctttagt tattttatta ctccagacaa ttggttaata ggtgaggatg tctgtttaca 3240

tggaatgaac agcaggagag taagaagttt aaaagaacca gataaataca accaagcggc 3300

acacatgaat gagtatgagt caatgccgat tacagaagaa tatgattggg gaggggttca 3360

tttcaatagc ggaataccta acaaagctgc ttataatacc atcacgaaag ttggtaggga 3420

acaggcagaa caactatatt ttcgagcatt aaagtattac ttaacgaaaa aatcccaatt 3480

tgtcgatgct aagaatgcgc ttcaacaggc ggcgagagac ttatacagcg aagaagtggc 3540

taaaaaagta ggagaagcat gggaagaagt tggcgttaga taagggatgt cctccaagcc 3600

aaattaacaa atgttaaaga aaagcggttt ctcccaatgc tattcttgaa gcaattcatg 3660

aattgttttc ctaaattagc taatagggag gttttaacaa gagtcaaaaa ctctaaacag 3720

cctgaattgt atactgttct tacgcaggta ttagagagcg gccgccagtg tgatggatat 3780

ctgcagaatt aattcggctt tctagagtga cttttatgtt gaggcattaa catttgttaa 3840

cgacgataaa gggacagcag gactagaata aagctataaa gcaagcatat aatattgcgt 3900

ttcatcttta gaagcgaatt tcgccaatat tataattatc aaaagagagg ggtggcaaac 3960

ggtatttggc attattaggt taaaaaatgt agaaggagag tgaaacccat gaaaaaaata 4020

atgctagttt ttattacact tatattagtt agtctaccaa ttgcgcaaca aactgaagca 4080

aaggatgcat cggatcttaa agctgtttat aattttgcta ctatgaagga tccatatcca 4140

tatgatgttc cagattatgc atcattgaat tcaatgagaa gtgaaagacc acaagctttg 4200

catcaaaaac gtacagcaat gttccaagat ccacaagaat ctggtcgtaa attaccacaa 4260

ttatgtacag aattacaaac aacaattcat gatattattt tagaatgtgt ttattgtaaa 4320

caacaattat tacgtcgtga agtttatgat ttcgcattcc gtgatttatg tattgtttat 4380

cgtgatggta acccatatgc agtttgtgat aaatgtttaa aattctattc taaaatttct 4440

gaatatcgtc atcatggtga tacaccaaca ttacatgaat atatgttaga tttacaacca 4500

gaaacaacag atttatatgg ttatggtcaa ttaaacgatt cttctgaaga agaagatgaa 4560

attgatggtc cagcaggtca agcagaacca gatcgtgcac attataacat tgttacattc 4620

tgttgtaaat gtgattctac attagataaa tgtttaaaat tctattctaa aatttctgaa 4680

tatcgtcatt attgttattc tgtttatggt acaacattag aacaacaata taacaaacca 4740

ttatgtgatt tattaattcg ttgtattaac tgtcaaaaac cattatgtcc agaagaaaaa 4800

caacgtcatt tagataaaaa acaacgtttc cataacattc gtggtcgttg gacaggtcgt 4860

tgtatgtctt gttgtcgttc ttctcgtaca cgtcgtgaaa cacaattaca ttataacatt 4920

gttacattct gttgtaaatg tgattctaca ttacgtttat gtgttcaatc tacacatgtt 4980

gatattcgta cattagaaga tttattaatg ggtacattag gtattgtttg tccaatttgt 5040

tctcaaaaac cactcgaggg attaaatgga ccagatattt ataaaggagt ttatcaattt 5100

aaaagtgttg aatttgatgt cgagtataca gatattgaaa tgaatagatt aggaaaatga 5160

taagtcggcg gccgcacaaa aaacggaaat cagttagtaa aactggtttc cgttttttat 5220

taatagtctt gagcctcaat aacaacagca gtgccagatg ctgtaaccat taacatacca 5280

ttatccgctc caagtacttc ataatcgata tccacaccaa ttacggcatt tgctccgata 5340

tctttggcgc gctgttccat ctctttaata gcttcctcac gtgcgttaat taactcgtct 5400

tcataacctt gtgatcgtcc accgaaaaag tttctgaggc cagctccaat gtctttcata 5460

aaattaacac cagtaattac ttctccgaaa acgatttttt tatattcgat aatttgcttg 5520

ccttcaatat ttggtgaggt agttacaatc attattaaat ccctccagtt ttcttttatc 5580

atacctctaa gttgtttttt tagtcaaagg atagctggtt attttttacg ggcgtgggct 5640

ttttcgatat aagtaatcgc atctgggact ttgcaagcag tttttcctaa atctacatat 5700

actttaccta ttgattctgc tgcttccaat gcttttgtgt aaagttcctc tttataaatt 5760

ccgcaagcga tgatataact attcatagag taacgagttc tgttcgtttc agattgtaaa 5820

gattcttttg cgagttccag taattcctcg gcttcaggca aagtcgcgat gtgttctgtg 5880

aaaatttgcc agcccattga tttagttaaa tcagcatcac ttttaatcca tttcctagca 5940

aaggtgagtg cgtcatcacg tttacttaca acggaggcta gcccaaaagt taacatcgtc 6000

caagcatctc gaaaagctat attccatttc tcgatttgtg aaacagctac ttttttaggg 6060

ttcacggcaa gtaaaccaag gtaaattaag tcgctattat tagactcgat taattggagt 6120

gctaattcgt gatttttatt caatttttca cggcgaataa ttttctttaa atcaccaatc 6180

tttaatctct agaacttttt taagtgtatc tgcggagttt ttcatttgtt ctttttcttt 6240

gtcatttaag ttcatttcaa caatatggcg aacgccttga cggttaacga ctgctggtgc 6300

acctatataa atatcgttca taccgtaatg gccatctaaa taaacagaaa gtggcaaaat 6360

cgcattttcg ttatttagaa ttgcttttgt aatacgagca agagctgcag caacgccgta 6420

gaatgtagcg ccttttttat taataatttc ataagctgca tcacgaacac ttacgaaaat 6480

agtatccatt gcaccttgtt catcttcgct aatccattca gtaattggaa ggccgccgac 6540

agttgtgtgg ctccatgctg ggaattcttg aagacgaaag ggcctcgtga tacgcctatt 6600

tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg 6660

aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 6720

catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 6780

tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 6840

tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 6900

ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 6960

ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga 7020

cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 7080

ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 7140

tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 7200

gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 7260

ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc 7320

aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 7380

acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 7440

tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 7500

cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 7560

gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 7620

taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 7680

tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 7740

cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 7800

ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 7860

accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 7920

cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 7980

cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 8040

tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 8100

taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 8160

gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 8220

agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 8280

ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 8340

acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 8400

caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 8460

tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 8520

tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct 8580

gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct 8640

cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt 8700

gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 8760

tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 8820

cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg 8880

tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc 8940

tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc 9000

tgtttggtca cttgatgcct ccgtgtaagg gggaatttct gttcatgggg gtaatgatac 9060

cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac 9120

tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca 9180

ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc 9240

agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca 9300

gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt 9360

tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa 9420

ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg 9480

gccaggaccc aacgctgccc gacgatgata agctgtcaaa catga 9525

<210> 15

<211> 23

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 15

gaagctaaaa gtgcaaatgt ccc 23

<210> 16

<211> 24

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 16

atttctttaa tactgcgttt gggg 24

Claims

1. The application of the recombinant attenuated listeria in preparing the vaccine for treating cervical cancer is characterized in that the recombinant attenuated listeria is recombinant attenuated listeria monocytogenes and recombinant attenuated listeria ovis, the recombinant attenuated listeria monocytogenes and the recombinant attenuated listeria ovis are respectively obtained by taking the attenuated listeria monocytogenes or the attenuated listeria ovis as carriers and carrying HPV16 type E6E7 fusion epitope peptide;

the attenuated listeria monocytogenes is obtained by completely knocking out two virulence genes namely actA and plcB from the listeria monocytogenes;

the attenuated sheep listeria is obtained by completely knocking out two virulence genes namely actA and plcB from the sheep listeria;

the administration mode of the vaccine is first injection and at least two times of boosting injection, and the recombinant attenuated Listeria monocytogenes and the recombinant attenuated sheep Listeria monocytogenes are sequentially and alternately injected.

2. The use of claim 1, wherein the recombinant attenuated listeria monocytogenes is prepared by the following method:

(1) synthesizing HPV16 type E6E7 fusion antigen epitope peptide gene fragments;

(2) constructing a targeting plasmid;

(3) preparing competent cells Lm delta actAplcB-lacZ;

3. The use of claim 1, wherein the recombinant attenuated listeria ovis is prepared by the following method:

(1) synthesizing HPV16 type E6E7 fusion antigen epitope peptide gene fragments;

(2) constructing a targeting plasmid;

(3) preparing a competent cell Li delta actAplcB-lacZ;