CN109006999A - A kind of bacitracin carbon dots/bacillus thuringiensis nano material preparation method and products thereof for capableing of fluorescence tracking, application - Google Patents
A kind of bacitracin carbon dots/bacillus thuringiensis nano material preparation method and products thereof for capableing of fluorescence tracking, application Download PDFInfo
- Publication number
- CN109006999A CN109006999A CN201810802543.3A CN201810802543A CN109006999A CN 109006999 A CN109006999 A CN 109006999A CN 201810802543 A CN201810802543 A CN 201810802543A CN 109006999 A CN109006999 A CN 109006999A
- Authority
- CN
- China
- Prior art keywords
- bacitracin
- bacillus thuringiensis
- carbon dots
- nano material
- capableing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 98
- 241000193388 Bacillus thuringiensis Species 0.000 title claims abstract description 92
- 229940097012 bacillus thuringiensis Drugs 0.000 title claims abstract description 92
- CLKOFPXJLQSYAH-ABRJDSQDSA-N bacitracin A Chemical compound C1SC([C@@H](N)[C@@H](C)CC)=N[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]1C(=O)N[C@H](CCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2N=CNC=2)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)NCCCC1 CLKOFPXJLQSYAH-ABRJDSQDSA-N 0.000 title claims abstract description 92
- 108010001478 Bacitracin Proteins 0.000 title claims abstract description 87
- 229960003071 bacitracin Drugs 0.000 title claims abstract description 87
- 229930184125 bacitracin Natural products 0.000 title claims abstract description 87
- 239000002086 nanomaterial Substances 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims abstract description 34
- 238000006243 chemical reaction Methods 0.000 claims abstract description 13
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 230000003260 anti-sepsis Effects 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 238000005119 centrifugation Methods 0.000 claims abstract description 5
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
- 239000002904 solvent Substances 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 7
- 238000004108 freeze drying Methods 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 7
- 238000004090 dissolution Methods 0.000 claims description 4
- 230000002349 favourable effect Effects 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 238000001027 hydrothermal synthesis Methods 0.000 abstract description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 9
- 229910052799 carbon Inorganic materials 0.000 abstract description 8
- 235000013305 food Nutrition 0.000 abstract description 5
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 23
- 239000007864 aqueous solution Substances 0.000 description 13
- 239000000463 material Substances 0.000 description 12
- 241000209094 Oryza Species 0.000 description 10
- 235000007164 Oryza sativa Nutrition 0.000 description 10
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 10
- 239000004810 polytetrafluoroethylene Substances 0.000 description 10
- 235000009566 rice Nutrition 0.000 description 10
- -1 polytetrafluoroethylene Polymers 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 5
- 239000008367 deionised water Substances 0.000 description 5
- 229910021641 deionized water Inorganic materials 0.000 description 5
- 238000010907 mechanical stirring Methods 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 238000001291 vacuum drying Methods 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000010189 synthetic method Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000010000 carbonizing Methods 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 125000000524 functional group Chemical group 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000002189 fluorescence spectrum Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000002096 quantum dot Substances 0.000 description 2
- 238000006862 quantum yield reaction Methods 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000001241 arc-discharge method Methods 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000006056 electrooxidation reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 229910021389 graphene Inorganic materials 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000000608 laser ablation Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000004227 thermal cracking Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
- A23B7/155—Microorganisms; Enzymes; Antibiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nanotechnology (AREA)
- Polymers & Plastics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Optics & Photonics (AREA)
- Materials Engineering (AREA)
- Inorganic Chemistry (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Manufacturing & Machinery (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of bacitracin carbon dots/bacillus thuringiensis nano material preparation methods for capableing of fluorescence tracking and products thereof, application comprising, it dissolves bacitracin: bacitracin is dissolved in solvent；Prepare carbon dots: 150~200 DEG C at a temperature of heating reaction 4~for 24 hours；It mixes bacillus thuringiensis: bacillus thuringiensis, stirring is added；Centrifugation, filtering.The present invention is carbon source using relatively inexpensive bacitracin, it does not need that any surface passivator is added, using current most popular one step hydro thermal method, can preparation can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) natural antisepsis fresh-keeping agent, experimental procedure is simple, fluorescence property, which is stablized, to be protruded, and antibacterial functions are obvious, brings Gospel for the existing fresh-keeping problem in fruit-vegetable food market.
Description
Technical field
The invention belongs to antistaling agent preparation technical fields, and in particular to a kind of bacitracin carbon dots/Soviet Union for capableing of fluorescence tracking
Preparation method of cloud gold bacillus nano material and products thereof, application.
Background technique
The quality of food and safe the problem of always food enterprise and consumption are paid special attention to, the growth and breeding of microorganism is not
It is only the major reason for causing product to rot, and part microorganism can also cause very big injury to human body.Especially
Whens using preservative film packaging fruits and vegetables etc., package interior air circulation is poor, and ambient humidity is big, it is easy to which strong-willed microorganism growth is numerous
It grows, so, preventing product by microbial contamination using antimicrobial antistaling agent, it is very important with Shelf-life is drilled.
Carbon quantum dot is exactly the carbon nanomaterial that a kind of size is less than 10nm.Generally comprise graphene quantum dot, carbon nanometer
Point and polymer quantum dot.Carbon dots preparation, property and in terms of research have been achieved for very big progress and gradually
As a nova in carbon nanomaterial family.The preparation method of carbon quantum dot has obtained quick development, cheap and easy to get
Raw material and diversified preparation means are greatly enriched the theoretical research and practical application of carbon dots.The synthesis of carbon quantum dot at present
Method can be generally divided into two major classes: method and from bottom to top method from top to bottom.
Summary of the invention
The purpose of this section is to summarize some aspects of the embodiment of the present invention and briefly introduce some preferable implementations
Example.It may do a little simplified or be omitted to avoid our department is made in this section and the description of the application and the title of the invention
Point, the purpose of abstract of description and denomination of invention it is fuzzy, and this simplification or omit and cannot be used for limiting the scope of the invention.
Therefore, as one aspect of the present invention, the present invention overcomes the deficiencies in the prior art, provides a kind of energy
Bacitracin carbon dots/bacillus thuringiensis nano material preparation method of enough fluorescence trackings.
In order to solve the above technical problems, the present invention provides the following technical scheme that a kind of bacitracin for capableing of fluorescence tracking
Carbon dots/bacillus thuringiensis nano material preparation method comprising,
Dissolution bacitracin: bacitracin is dissolved in solvent；
Prepare carbon dots: 150~200 DEG C at a temperature of heating reaction 4~for 24 hours；
It mixes bacillus thuringiensis: bacillus thuringiensis, stirring is added；Centrifugation, filtering.
As the bacitracin carbon dots/bacillus thuringiensis nano material preparation of the present invention for capableing of fluorescence tracking
A kind of preferred embodiment of method: the dissolution bacitracin, wherein the solvent includes water, and the ratio of the bacitracin and water is
Every 1g bacitracin is dissolved in 40~80mL water.
As the bacitracin carbon dots/bacillus thuringiensis nano material preparation of the present invention for capableing of fluorescence tracking
A kind of preferred embodiment of method: the preparation carbon dots, wherein the heating reaction, temperature are 180 DEG C, time 10h.
As the bacitracin carbon dots/bacillus thuringiensis nano material preparation of the present invention for capableing of fluorescence tracking
A kind of preferred embodiment of method: the cooling is to be cooled to room temperature.
As the bacitracin carbon dots/bacillus thuringiensis nano material preparation of the present invention for capableing of fluorescence tracking
A kind of preferred embodiment of method: the centrifugation, revolving speed are 11000~13000rpm, and the time is 6~10 min.
As the bacitracin carbon dots/bacillus thuringiensis nano material preparation of the present invention for capableing of fluorescence tracking
A kind of preferred embodiment of method: the stirring, mixing speed are 400~600r/min.
As the bacitracin carbon dots/bacillus thuringiensis nano material preparation of the present invention for capableing of fluorescence tracking
A kind of preferred embodiment of method: the addition bacillus thuringiensis, the matter of the bacillus thuringiensis and bacitracin carbon dots
Amount is than being 0.1:2.5~3.5.
As the bacitracin carbon dots/bacillus thuringiensis nano material preparation of the present invention for capableing of fluorescence tracking
A kind of preferred embodiment of method: further including,
Freeze-drying: its temperature is -60~-50 DEG C, and vacuum degree is 9~10Pa, and the processing time is 18~26h.
As another aspect of the present invention, the present invention overcomes the deficiencies in the prior art, and providing one kind can be glimmering
Bacitracin carbon dots/bacillus thuringiensis nano material of light tracking.
In order to solve the above technical problems, the present invention provides the following technical scheme that a kind of bacitracin for capableing of fluorescence tracking
Carbon dots/bacillus thuringiensis nano material, in which: the bacitracin carbon dots/bacillus thuringiensis nano material is in water
Favorable dispersibility, average grain diameter are 4~5nm, and Zeta potential is -19.6mv.
As another aspect of the present invention, the present invention overcomes the deficiencies in the prior art, provide can fluorescence chase after
The bacitracin carbon dots of track/application of the bacillus thuringiensis nano material as natural antisepsis fresh-keeping agent.
In order to solve the above technical problems, the present invention provides the following technical scheme that the bacillus for capableing of fluorescence tracking
Peptide carbon dots/application of the bacillus thuringiensis nano material as natural antisepsis fresh-keeping agent.
Beneficial effects of the present invention: the present invention is carbon source using relatively inexpensive bacitracin, does not need that any table is added
Face passivator, using current most popular one step hydro thermal method, can prepare can bacitracin carbon dots/Su Yunjin for tracking of fluorescence
Bacillus (Bt-CQDs) natural antisepsis fresh-keeping agent, experimental procedure is simple, and fluorescence property, which is stablized, to be protruded, and antibacterial functions are obvious, are
The existing fresh-keeping problem in fruit-vegetable food market brings Gospel.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, required use in being described below to embodiment
Attached drawing be briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for this
For the those of ordinary skill of field, without any creative labor, it can also be obtained according to these attached drawings other
Attached drawing.Wherein:
Fig. 1 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) receive
The transmission electron microscope picture of rice material.
Fig. 2 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) receive
The grain size distribution of rice material.
Fig. 3 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) receive
The Zeta potential spectrogram of rice material.
Fig. 4 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) nanometer
The fluorescence spectra of material Bt-CQDs in different pH solution.
Fig. 5 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) receive
The growth result figure of bacillus thuringiensis on the solid medium of the Bt-CQDs of the various concentration of the aqueous solution of rice material.
Fig. 6 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) receive
Equivalent Escherichia coli the consolidating in various concentration bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) of the aqueous solution of rice material
Growth result on body culture medium.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, right combined with specific embodiments below
A specific embodiment of the invention is described in detail.
In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention, but the present invention can be with
Implemented using other than the one described here other way, those skilled in the art can be without prejudice to intension of the present invention
In the case of do similar popularization, therefore the present invention is not limited by the specific embodiments disclosed below.
Secondly, " one embodiment " or " embodiment " referred to herein, which refers to, may be included at least one realization side of the invention
A particular feature, structure, or characteristic in formula." in one embodiment " that different places occur in the present specification not refers both to
The same embodiment, nor the individual or selective embodiment mutually exclusive with other embodiments.
Embodiment 1:
It is a kind of can fluorescence tracking the preparation of bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) nano material:
Step 1, the bacitracin powder for weighing 0.5g is placed in the clean beaker of 50mL, and the deionized water of 30mL is added, complete
Fully dissolved obtains colorless and transparent aqueous solution.
Step 2, colourless solution is transferred in polytetrafluoroethylene (PTFE) hydrothermal reaction kettle, is placed in a vacuum drying oven, at 180 DEG C
Lower heated at constant temperature 10h, it is cooling.
Step 3, after reaction, it is added suitable bacillus thuringiensis, under normal temperature condition, carries out mechanical stirring 4h.
Step 4, obtained turbid solution is placed in a centrifuge and 10min, filtering is centrifuged with the revolving speed of 12000r/min.
Step 5, bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) solution will be obtained and pass through vacuum freeze drier
Freeze-drying (temperature be -60~-50 DEG C, vacuum degree be 9~10Pa, the processing time be 18~26h) obtain can fluorescence track bacillus
Peptide carbon dots/bacillus thuringiensis (Bt-CQDs) nano material powder.
Embodiment 2:
It is a kind of can fluorescence tracking the preparation of bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) nano material:
Step 1, the bacitracin powder for weighing 0.5g is placed in the clean beaker of 50mL, and the deionized water of 30mL is added, complete
Fully dissolved obtains colorless and transparent aqueous solution.
Step 2, colourless solution is transferred in polytetrafluoroethylene (PTFE) hydrothermal reaction kettle, is placed in a vacuum drying oven, at 180 DEG C
Lower heated at constant temperature 4h, it is cooling.
Step 3, after reaction, it is added suitable bacillus thuringiensis, under normal temperature condition, carries out mechanical stirring 4h.
Step 4, obtained turbid solution is placed in a centrifuge and 10min, filtering is centrifuged with the revolving speed of 12000r/min.
Step 5, bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) solution will be obtained and pass through vacuum freeze drier
Freeze-drying (temperature be -60~-50 DEG C, vacuum degree be 9~10Pa, the processing time be 18~26h) obtain can fluorescence track bacillus
Peptide carbon dots/bacillus thuringiensis (Bt-CQDs) nano material powder.
Embodiment 3:
It is a kind of can fluorescence tracking the preparation of bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) nano material:
Step 1, the bacitracin powder for weighing 0.5g is placed in the clean beaker of 50mL, and the deionized water of 30mL is added, complete
Fully dissolved obtains colorless and transparent aqueous solution.
Step 2, colourless solution is transferred in polytetrafluoroethylene (PTFE) hydrothermal reaction kettle, is placed in a vacuum drying oven, at 180 DEG C
Lower heated at constant temperature 6h, it is cooling.
Step 3, after reaction, it is added suitable bacillus thuringiensis, under normal temperature condition, carries out mechanical stirring 4h.
Step 4, obtained turbid solution is placed in a centrifuge and 10min, filtering is centrifuged with the revolving speed of 12000r/min.
Step 5, bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) solution will be obtained and pass through vacuum freeze drier
(temperature is -60~-50 DEG C, and vacuum degree is 9~10Pa, and the processing time is 18~26h for freeze-drying.) obtain can fluorescence tracking bar
Bacterium peptide carbon dots/bacillus thuringiensis (Bt-CQDs) nano material powder.
Embodiment 4:
It is a kind of can fluorescence tracking the preparation of bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) nano material:
Step 1, the bacitracin powder for weighing 0.5g is placed in the clean beaker of 50mL, and the deionized water of 30mL is added, complete
Fully dissolved obtains colorless and transparent aqueous solution.
Step 2, colourless solution is transferred in polytetrafluoroethylene (PTFE) hydrothermal reaction kettle, is placed in a vacuum drying oven, at 180 DEG C
Lower heated at constant temperature 8h, it is cooling.
Step 3, after reaction, suitable bacillus thuringiensis, the bacillus thuringiensis and bacitracin is added
The mass ratio of carbon dots is 0.1:2.5~3.5, under normal temperature condition, carries out mechanical stirring 4h.
Step 4, obtained turbid solution is placed in a centrifuge and 10min, filtering is centrifuged with the revolving speed of 12000r/min.
Step 5, bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) solution will be obtained and pass through vacuum freeze drier
Freeze-drying (temperature be -60~-50 DEG C, vacuum degree be 9~10Pa, the processing time be 18~26h) obtain can fluorescence track bacillus
Peptide carbon dots/bacillus thuringiensis (Bt-CQDs) nano material powder.
Embodiment 5:
It is a kind of can fluorescence tracking the preparation of bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) nano material:
Step 1, the bacitracin powder for weighing 0.5g is placed in the clean beaker of 50mL, and the deionized water of 30mL is added, complete
Fully dissolved obtains colorless and transparent aqueous solution.
Step 2, colourless solution is transferred in polytetrafluoroethylene (PTFE) hydrothermal reaction kettle, is placed in a vacuum drying oven, at 180 DEG C
Lower heated at constant temperature is for 24 hours, cooling.
Step 3, after reaction, it is added suitable bacillus thuringiensis, under normal temperature condition, carries out mechanical stirring 4h.
Step 4, obtained turbid solution is placed in a centrifuge and 10min, filtering is centrifuged with the revolving speed of 12000r/min.
Step 5, bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) solution will be obtained and pass through vacuum freeze drier
(temperature is -60~-50 DEG C, and vacuum degree is 9~10Pa, and the processing time is 18~26h for freeze-drying.) obtain can fluorescence tracking bar
Bacterium peptide carbon dots/bacillus thuringiensis (Bt-CQDs) nano material powder.
Table 1 at 180 DEG C the hydro-thermal method reaction time to can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis
(Bt-CQDs) influence of nano material fluorescence intensity
As seen from the table at 180 DEG C hydro-thermal method synthesis can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis
(Bt-CQDs) optimum reacting time of nano material is 10h.
It grinds and is studied carefully discovery, reaction temperature determines carbonizing degree of the reactant in carbon dots forming process, and reaction temperature is excessively high,
Carbonizing degree is too high, and the carbon dots surface functional group content of system is too low；Reaction temperature is too low, and carbon dots carbonizing degree is too low, system
Carbon dots surface functional group too high levels；And the value volume and range of product of carbon dots surface functional group, directly affect its fluorescence quantum yield
And optical property.Synthesis can bacitracin carbon dots/bacillus thuringiensis (Bt- for tracking of fluorescence at 180 DEG C known in upper table
CQDs) optimum reacting time of nano material is 10h.When temperature is 180 DEG C, and the reaction time is 10h, for preparing can fluorescence
The fluorescence quantum yield highest of the bacitracin carbon dots of tracking.
Fig. 1 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) receive
The transmission electron microscope picture of rice material；Shown in figure can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs)
Nano material favorable dispersibility, it is uniform, do not reunite.
Fig. 2 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) receive
The grain size distribution of rice material；Shown in figure can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs)
Nano material average grain diameter is 4.8nm.
It has been investigated that the ratio of step 1 bacitracin and water, the time of step 3 stirring, step 4 are centrifuged in the present invention
Condition all can size on carbon dots and pattern make a significant impact, using the combination of the selected parameter of the present invention, so that preparation
Carbon dots average grain diameter reach 4.8nm, it is uniform, be uniformly dispersed, do not reunite.
Fig. 3 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) receive
The Zeta potential spectrogram of rice material；Shown in figure can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-
CQDs) nano material is averaged Zeta potential as -19.6mv.The characterize data illustrates that Bt-CDs aqueous solution can be stabilized.
Fig. 4 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) nanometer
The fluorescence spectra of material Bt-CQDs in different pH solution；When pH is 1 and 13, Bt-CQDs aqueous solution is almost without glimmering
The fluorescence of luminous intensity, Bt-CQDs is destroyed in both solution in property.During increase of the pH by 3 to 9, although
The fluorescence intensity of Bt-CQDs is weakened in the solution that pH is 5, but the fluorescence intensity of Bt-CQDs aqueous solution is in totally with pH
The raising downward trend of value, and in the solution environmental that pH is 3, Bt-CQDs aqueous solution has maximum fluorescence intensity.
Fig. 5 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) receive
The growth result of bacillus thuringiensis on the solid medium of the Bt-CQDs of the various concentration of the aqueous solution of rice material.Containing
Have in bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) culture medium, such as Fig. 5, it is known that with bacitracin carbon dots/
The clump count of the increase of bacillus thuringiensis (Bt-CQDs) solution concentration, the bacillus thuringiensis on solid medium increases
Add, illustrating bacitracin carbon dots not influences growth of the bacillus thuringiensis on solid medium, and the two has Synergistic antimicrobial
Effect.
Fig. 6 be the embodiment of the present invention 1 can fluorescence tracking bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) receive
Equivalent Escherichia coli the consolidating in various concentration bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) of the aqueous solution of rice material
Growth result on body culture medium.Containing in bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) culture medium, such as scheme
6, from bacteriostatic experiment it is observed that relative to individual bacitracin, bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs)
Carbon dots with apparent antibacterial effect, the absorption bacillus thuringiensis with Nano grade size are easy to Escherichia coli
With breeding generate inhibiting effect, and bacitracin carbon dots absorption bacillus thuringiensis minimal inhibitory concentration be 0.5 mg/
mL.It is 118 × 5 × 10 for concentration18=5.90 × 1020The Escherichia coli of cfu/g observe bacillus after being incubated for 24 hours
Peptide carbon dots adsorb bacteriostasis rate of the bacillus thuringiensis in 0.5mg/mL and reach 99.75% or more.
The synthetic method of carbon quantum dot is segmented into two major classes: synthetic method and from bottom to top synthetic method from top to bottom, from upper
Method under and is primarily referred to as cracking small sized carbon quantum dot from large-sized carbon source, and main method includes arc discharge
Method, electrochemical oxidation process and laser ablation method etc.；And synthetic method from bottom to top is primarily referred to as making with small molecule compound
Carbonization or thermal cracking are carried out for carbon source, it is carbon source using relatively inexpensive bacitracin that method, which synthesizes, from bottom to top for present invention selection,
It does not need that any surface passivator is added, using current most popular one step hydro thermal method, can prepare can fluorescence tracking
Bacitracin carbon dots/bacillus thuringiensis (Bt-CQDs) natural antisepsis fresh-keeping agent, experimental procedure is simple, fluorescence property stablize
Prominent, antibacterial functions are obvious, bring Gospel for the existing fresh-keeping problem in fruit-vegetable food market.
It should be noted that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to preferable
Embodiment describes the invention in detail, those skilled in the art should understand that, it can be to technology of the invention
Scheme is modified or replaced equivalently, and without departing from the spirit and scope of the technical solution of the present invention, should all be covered in this hair
In bright scope of the claims.
Claims (10)
1. one kind is capable of bacitracin carbon dots/bacillus thuringiensis nano material preparation method of fluorescence tracking, feature exists
In: including,
Dissolution bacitracin: bacitracin is dissolved in solvent；
Prepare carbon dots: 150~200 DEG C at a temperature of heating reaction 4~for 24 hours；
It mixes bacillus thuringiensis: bacillus thuringiensis, stirring is added；Centrifugation, filtering.
2. capableing of bacitracin carbon dots/bacillus thuringiensis nano material preparation of fluorescence tracking as described in claim 1
Method, it is characterised in that: the dissolution bacitracin, wherein the solvent includes water, and the ratio of the bacitracin and water is every 1g
Bacitracin is dissolved in 40~80mL water.
3. capableing of bacitracin carbon dots/bacillus thuringiensis nano material system of fluorescence tracking as claimed in claim 1 or 2
Preparation Method, it is characterised in that: the preparation carbon dots, wherein the heating reaction, temperature are 180 DEG C, time 10h.
4. capableing of bacitracin carbon dots/bacillus thuringiensis nano material system of fluorescence tracking as claimed in claim 1 or 2
Preparation Method, it is characterised in that: the cooling is to be cooled to room temperature.
5. capableing of bacitracin carbon dots/bacillus thuringiensis nano material system of fluorescence tracking as claimed in claim 1 or 2
Preparation Method, it is characterised in that: the centrifugation, revolving speed are 11000~13000rpm, and the time is 6~10min.
6. capableing of bacitracin carbon dots/bacillus thuringiensis nano material system of fluorescence tracking as claimed in claim 1 or 2
Preparation Method, it is characterised in that: the stirring, mixing speed are 400~600r/min.
7. capableing of bacitracin carbon dots/bacillus thuringiensis nano material system of fluorescence tracking as claimed in claim 1 or 2
Preparation Method, it is characterised in that: the addition bacillus thuringiensis, the quality of the bacillus thuringiensis and bacitracin carbon dots
Than for 0.1:2.5~3.5.
8. capableing of bacitracin carbon dots/bacillus thuringiensis nano material system of fluorescence tracking as claimed in claim 1 or 2
Preparation Method, it is characterised in that: further include,
Freeze-drying: its temperature is -60~-50 DEG C, and vacuum degree is 9~10Pa, and the processing time is 18~26h.
9. bacitracin carbon dots/bacillus thuringiensis nano material that one kind is capable of fluorescence tracking, it is characterised in that: the bacillus
Peptide carbon dots/bacillus thuringiensis nano material favorable dispersibility in water, average grain diameter are 4~5nm, Zeta potential is-
19.6mv。
10. any bacitracin carbon dots/bacillus thuringiensis nano material for capableing of fluorescence tracking of claim 1~9
Application as natural antisepsis fresh-keeping agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810802543.3A CN109006999A (en) | 2018-07-20 | 2018-07-20 | A kind of bacitracin carbon dots/bacillus thuringiensis nano material preparation method and products thereof for capableing of fluorescence tracking, application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810802543.3A CN109006999A (en) | 2018-07-20 | 2018-07-20 | A kind of bacitracin carbon dots/bacillus thuringiensis nano material preparation method and products thereof for capableing of fluorescence tracking, application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109006999A true CN109006999A (en) | 2018-12-18 |
Family
ID=64643704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810802543.3A Pending CN109006999A (en) | 2018-07-20 | 2018-07-20 | A kind of bacitracin carbon dots/bacillus thuringiensis nano material preparation method and products thereof for capableing of fluorescence tracking, application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109006999A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110269093A (en) * | 2019-07-19 | 2019-09-24 | 云南省农业科学院农产品加工研究所 | A kind of fresh-cut fruit and vegetable fresh-keeping liquid |
| CN110618116A (en) * | 2019-08-28 | 2019-12-27 | 江苏大学 | Preparation method and application of intelligent indication label for visually detecting freshness of meat |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103109867A (en) * | 2013-02-19 | 2013-05-22 | 中国科学院理化技术研究所 | Application of heteroatom doped multifunctional carbon quantum dot serving as photosensitizer in antimicrobial material |
-
2018
- 2018-07-20 CN CN201810802543.3A patent/CN109006999A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103109867A (en) * | 2013-02-19 | 2013-05-22 | 中国科学院理化技术研究所 | Application of heteroatom doped multifunctional carbon quantum dot serving as photosensitizer in antimicrobial material |
Non-Patent Citations (2)
| Title |
|---|
| PENG HOU ET AL.: ""An active structure preservation method for developing functional graphitic carbon dots as an effective antibacterial agent and a sensitive pH and Al(III) nanosensor"", 《NANOSCALE》 * |
| 郭颖等: ""碳点的制备及在荧光分析中的应用"", 《理化检验-化学分册》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110269093A (en) * | 2019-07-19 | 2019-09-24 | 云南省农业科学院农产品加工研究所 | A kind of fresh-cut fruit and vegetable fresh-keeping liquid |
| CN110618116A (en) * | 2019-08-28 | 2019-12-27 | 江苏大学 | Preparation method and application of intelligent indication label for visually detecting freshness of meat |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jayaramudu et al. | Chitosan-pluronic based Cu nanocomposite hydrogels for prototype antimicrobial applications | |
| Qi et al. | Hydroxyapatite hierarchically nanostructured porous hollow microspheres: rapid, sustainable microwave‐hydrothermal synthesis by using creatine phosphate as an organic phosphorus source and application in drug delivery and protein adsorption | |
| Emamifar et al. | Effect of nanocomposite packaging containing Ag and ZnO on inactivation of Lactobacillus plantarum in orange juice | |
| Rai et al. | Biogenic nanoparticles: an introduction to what they are, how they are synthesized and their applications | |
| CN103734188A (en) | Preparation method and applications of zinc oxide-graphene oxide composite nanomaterial | |
| CN109006999A (en) | A kind of bacitracin carbon dots/bacillus thuringiensis nano material preparation method and products thereof for capableing of fluorescence tracking, application | |
| CN112472863B (en) | Negative ion functional chip, preparation method and negative ion sanitary towel | |
| Salunke et al. | Potential of Kalopanax septemlobus leaf extract in synthesis of silver nanoparticles for selective inhibition of specific bacterial strain in mixed culture | |
| Pattanshetti et al. | Synthesis of multi-walled carbon nanotubes (MWCNTs) from plastic waste & analysis of garlic coated gelatin/MWCNTs nanocomposite films as food packaging material | |
| Jose et al. | Fabrication, characterization and in vitro antifungal property evaluation of biocompatible lignin-stabilized zinc oxide nanoparticles against selected pathogenic fungal strains | |
| Liu et al. | Thymol-functionalized hollow mesoporous silica spheres nanoparticles: preparation, characterization and bactericidal activity | |
| CN109481678B (en) | Organic-inorganic hybrid composite silver nanoparticles and preparation method and application thereof | |
| Huang et al. | A review of selenium (Se) nanoparticles: from synthesis to applications | |
| Roy et al. | Recent advances in metal sulfide nanoparticle-added bionanocomposite films for food packaging applications | |
| CN108249420B (en) | A kind of positively charged carbon dots and its preparation method and application | |
| CN114027322A (en) | Biochar-based bacillus thuringiensis sustained-release microspheres as well as preparation method and application thereof | |
| WO2023227145A1 (en) | Preparation method for platinum nanoparticles, platinum nanoparticles prepared therefrom, and application of platinum nanoparticles | |
| CN108753281A (en) | A kind of preparation method of bacitracin carbon dots nano material and products thereof that capableing of fluorescence tracking, application | |
| CN108213459B (en) | Preparation method of glucan/nano gold-silver alloy compound | |
| Zihan et al. | Starch nanoparticles as a new ice crystal nucleator in Lactobacillus bulgaricus CICC 6097 cryoprotection | |
| CN103147308B (en) | A kind of squeeze protective agent and preparation method thereof | |
| CN114106823B (en) | Preparation method of quaternary ammonium salt curcumin carbon quantum dot and broad-spectrum antibacterial application | |
| CN114160785B (en) | Preparation method of mild condition controllable nano silver solution | |
| CN107114405A (en) | Nano Silver/hydroxide nanoparticle assembling ball compound disinfectant and preparation method thereof | |
| JP2013177678A (en) | Composite of silver fine particle and dna and method for producing the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181218 |