CN109003673A - A kind of method and skin care method of the active constituent and skin care item for recommending to be suitable for individual - Google Patents
A kind of method and skin care method of the active constituent and skin care item for recommending to be suitable for individual Download PDFInfo
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- CN109003673A CN109003673A CN201710416435.8A CN201710416435A CN109003673A CN 109003673 A CN109003673 A CN 109003673A CN 201710416435 A CN201710416435 A CN 201710416435A CN 109003673 A CN109003673 A CN 109003673A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/148—Screening for cosmetic compounds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Abstract
The invention discloses the methods and skin care method of a kind of active constituent and skin care item for recommending to be suitable for individual, are related to skin care service field.The method of the active constituent disclosed by the invention for recommending to be suitable for individual is based on leading to single nucleotide polymorphism of the hereditary immanent cause of skin problem i.e. with the specific site on skin characteristic related gene, solution from the skin care item containing corresponding active constituent to individual that recommend active constituent or overcomes the problems, such as existing to recommend targeting existing for active constituent poor according only to dermal phenotype；And hierarchical classification and assessment are carried out to active constituent using research conditions of the existing literature to the active constituent of recommendation simultaneously, so that the skin care solution recommended is with more systematicness and science.
Description
Technical field
The present invention relates to skin care service fields, and individual active constituent and skin care item are suitable in particular to recommendation
Method and skin care method.
Background technique
Currently, with the development of science and technology, skin care item industry, as the rising industry of high added value, towards more section
It learns, multiplicity is high-end, customizes direction and develops.Especially in recent years, for individual's skin feature, some skin care item manufacturers or beauty
Hold the evaluation measures come into effect for skin characteristic, while recommending or producing the product for being directed to its skin speciality, reaches user
The concept of skin care item privatization customization.
Active constituent is also known as effective component, refers to the Skin Cell that acts on added in the basic ingredient of skin care item
Ingredient with physiological activity or medical functions.In skin care field, active constituent is real or unique energy to a certain extent
Enough change the ingredient of cell effect, all core substances for being often referred to as entire product.Simultaneously because of every kind of active constituent, function
The difference of effect, source is different, and also there is a big difference for cost, and larger specific gravity is often accounted in the cost of entire product, is also become
The core of skin care item publicity.It is directed to the hereditary difference of each individual, the metabolism of body and the functional character of Skin Cell are because of people
And it is different.Therefore, active constituent is selected with greater need for the speciality for individual, can be only achieved preferable skin effect.
Existing many customization services for being directed to skin care are detected for dermal phenotype, such as skin image
Acquisition, questionnaire survey show that skin type is assessed, then recommend the solution of solution skin problem according to assessment result, this
This is not recommended kind method without individual immanent cause for skin problem, the targeting effect being often extremely difficult to
Fruit.
Summary of the invention
The purpose of the present invention is to provide a kind of method of active constituent for recommending to be suitable for individual, this method is asked for skin
The inherent genetic cause of topic be the diversity based on the SNP site on skin characteristic related gene, recommend out be suitable for individual, most
Excellent improves the active constituent of skin characteristic, and then reaches good targeting skin effect.
Another object of the present invention is to provide a kind of methods of skin care item for recommending to be suitable for individual, and this method is according to above-mentioned
The obtained recommendation results of method for being suitable for the active constituent of individual are recommended to recommend to the individual containing the active constituent
Skin care item.
A further object of the present invention is to provide a kind of skin care method, this method is suitable for the activity of individual according to above-mentioned recommendation
The obtained recommendation results of the method for ingredient contact the skin of the individual using the skin care item containing the active constituent.
The present invention is implemented as follows:
A method of recommending the active constituent for being suitable for individual, the active constituent is configured to the primary raw material of skin care item,
Comprising:
The genotype for detecting the SNP site to be measured on the target gene of individual, obtains the genotype to the target base
Because of the influence mode of expression, the target gene is related to skin characteristic；
Retrieval is it has been reported that active constituent has the document of regulating and controlling effect to the expression of the target gene, in the document report
Pre- recommendation active constituent is selected in the active constituent type in road, what the pre- recommendation active constituent expressed the target gene
Regulating and controlling effect and the genotype generate supplementary function to the influence mode that the target gene is expressed；
It is arranged according to the pre- height descending for recommending the correlation between active constituent and target gene expression described in every kind
Column, as the recommendation results to the individual.
A method of recommending the skin care item for being suitable for individual, the side of the active constituent of individual is suitable for according to above-mentioned recommendation
The obtained recommendation results of method recommend the skin care item for containing the active constituent to individual.
A kind of skin care method is suitable for the obtained recommendation results of method of the active constituent of individual according to above-mentioned recommendation
The skin of the individual is contacted using the skin care item containing the active constituent.
Beneficial effects of the present invention for example,
The method of the active constituent provided by the invention for recommending to be suitable for individual, this method is based on individual target gene
The genotype of SNP site to be measured obtains the influence mode that genotype expresses target gene, target gene and skin characteristic phase
It closes, determines the inherent genetic cause for generating skin problem, be to be selected in disclosed document report further according to existing research evidence
Regulating and controlling effect and genotype to target gene expression generate the pre- recommendation of supplementary function to the influence mode that target gene is expressed
Active constituent is made after being arranged according to the height descending of the correlation between the pre- recommendation active constituent selected and target gene expression
For to individual recommendation results.The active constituent recommended is directed to the inherent genetic cause of individual's skin problem, with higher
Targeting, and the correlation between selected pre- recommendation active constituent and target gene expression is high, has obtained existing literature
Strong support and explanation, recommendation results systematicness with higher and science.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the pyramid pattern figure (figure of the research type level height for the active constituent that the embodiment of the present invention 1 provides
In: level is from high in the end successively are as follows: human colony's research, mankind human research, zooscopy, human cells in vitro's research, body
Outer zooblast research, the research of external biochemical test).
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.The active constituent of individual and the method and skin care of skin care item are suitable for the recommendation of the embodiment of the present invention below
Method is specifically described.
The discovery multi-source of gene specific site relevant to skin characteristic is in whole-genome association (Genome-wide
Association study, GWAS), GWAS method generally by a large amount of groups carry out full genome detection, look for it is certain
Specific site on the relevant genome of skin characteristic, nucleotide diversity (the Single Nucleotide in these sites
Polymorphisms, SNP) pass through correlation statistically and detects, it was demonstrated that its (such as obscure, black, aging, oxygen with skin characteristic
Change, drying, allergy, wrinkle, acne, fat granule and fuel-displaced) correlation, and the gene that its site is located at, also having can
It can report related to skin characteristic.These on detection part genome are generally used for the relevant genetic test of skin characteristic
Site, the nucleotide by analyzing these specific sites change (i.e. genotype), infer the essence of individual inheritance, assess skin shape
Then condition proposes skin care scheme.Such skin care scheme has better specific aim and targeting.
Based on this, on the one hand, the present invention provides a kind of method of active constituent for recommending to be suitable for individual, the work recommended
Property ingredient is configured to the primary raw material of skin care item comprising:
Step S1: the genotype of the SNP site to be measured on target gene relevant to skin characteristic on detection individual obtains
The influence mode for taking genotype to express target gene.
By genotype, that is, nucleotide type of the upper SNP site to be measured relevant to skin characteristic of detection individual, can define
The influence mode (can report that inquiry obtains according to existing literature) that the genotype of SNP site to be measured expresses target gene, in turn
It determines the inherent genetic cause of the individual for generating skin problem, proposes that the skin care scheme for genetic cause is asked to solve skin
Topic has more targeting so that the active constituent recommended is with strong points, is conducive to improve skin effect.
Usually, the genotype of SNP site to be measured is to express target gene to the influence mode that target gene is expressed
It raises, target gene expression is made to lower or make target gene normal expression.
Wherein, in some embodiments of the present invention, the type of skin characteristic includes black, aging, oxidation, wrinkle, Cuo
One or more of sore.Certainly, the type of skin characteristic may also include the classes such as drying, allergy, obscure, fuel-displaced and fat granule
Type.During actual recommendation, specific skin characteristic target gene relevant with its can be according to the skin problem of individual itself
It determines.
Wherein, it should be noted that SNP site can be found by reported whole-genome association (GWAS).
For example, in some embodiments of the present invention, target gene is TYR gene, MMP1 gene and SOD1 gene
Middle one kind；SNP site to be measured on TYR gene is rs10765198, and the SNP site to be measured on MMP1 gene is rs1799750,
SNP site to be measured on SOD1 gene is rs2070424.
These SNP sites change the state of cell, final shadow by the expression or function of its target gene being located in of influence
Ring the appearance of skin.
It should be noted that the target gene of present invention meaning is not limited to above-mentioned three kinds of genes (TYR gene, MMP1 base
Cause and SOD1 gene), it is also possible to other genes relevant to skin characteristic, as long as it, which exists, influences skin characteristic or lead
The SNP site for causing skin problem (black, aging, oxidation, wrinkle, acne, drying, allergy, obscure, fuel-displaced and fat granule etc.) to generate
This genoid, can be used as target gene of the invention.
TYR gene, that is, tyrosinase cdna, the generation of melanin polymer are considered pertaining only to a cuprase, referred to as junket
Propylhomoserin enzyme (TYR), the first step of tyrosinase catalysis melanin access key, i.e. tyrosine hydroxyl turn to DOPA；It is also assisted in
The reactions (PMID:6810464) of two subsequent steps.From going out as the result is shown for the melanocyte of different type Skins culture
Correlation between apparent melanin content and tyrosinase activity.Because the encoding gene of tyrosine is in different races
Different, Black people's skin tyrosinase activity is 10 times (PMID:9819560) of white man's skin.Rs10765198 is TYR gene
On SNP site to be measured, the diversity in the site be reported for the melanin deposition of skin have very strong correlation (PMID:
19340012).For carrying the individual of C nucleotide on this site (rs10765198), TYR enzyme expression, which can be better than, to be taken
Individual with T nucleotide leads to the function of stronger TYR enzyme, causes the generation of more melanin, the cutaneous colour of skin.
In addition, MMP1 gene encodes Fibroblast collagenase, the excessive damage for skin collagen matrix is skin light
One major reason of aging, by throughout the year chronic or randomness the expression for being exposed to the MMP1 gene caused by solar ultraviolet
The main reason for up-regulation is considered as collagen destruction or degradation.MMP1 is a main collagen degradation enzyme, it will lead to
The serious old problem of skin light decay (PMID:12929747).During skin aging and wrinkle generate, rs1799750 is base
Site on -1 gene of matter metalloproteinases (MMP1), its diversity, which has been reported, the aging of skin and wrinkle
Very strong correlation (PMID:9850057).Wherein, G core is carried for site (rs1799750) a certain on MMP1 gene promoter
The expression of the individual of thuja acid insertion mutation, MMP1 can be better than the individual for not carrying this nucleotide, lead to stronger matrix gold
The function of Proteases -1, causes the degradation of more collagens, and cutaneous aging shows (PMID:11557670).
In addition, SOD1 gene encoding superoxide dismutase, eliminates the nocuousness that organism generates in metabolic processes
Substance.It is the antioxidant enzyme for protecting the skin from peroxide pressure important in body.It is in scavenger-cell
Superoxide ion has played important function, these superoxide ions be generate into the cell active oxidation substance (PMID:
20352474).During skin oxidative, rs2070424 is the site to be measured on superoxide dismutase SOD1 gene, it
Diversity, which has been reported, has very deep influence (PMID:20352474) for the oxidation resistance of individual's skin.Wherein for
On SOD1 gene SNP site (rs2070424) carry A nucleotides inserted mutation individual, the expression of SOD1 be weaker than carry A this
The individual of one nucleotide leads to the reduction of superoxide dismutase, it is final weaken the oxidation resistant ability of skin (PMID:
22972774) skin, is caused to generate the skin problems such as aging and wrinkle.
Step S2: retrieval is it has been reported that active constituent has the document of regulating and controlling effect to the expression of above-mentioned target gene, upper
It states in the active constituent type of document (document retrieved) report and selects pre- recommendation active constituent, wherein is above-mentioned pre-
The influence for recommending active constituent to express above-mentioned target gene the regulating and controlling effect that above-mentioned target gene is expressed with said gene type
Mode generates supplementary function.
" supplementary function " means: if the genotype (nucleotide type) of SNP site to be measured expresses target gene
Influence mode be to make target gene expression up-regulation, then recommend the regulating and controlling effect expressed above-mentioned target gene of active constituent to be in advance
Inhibit the expression of target gene；If the influence mode that the genotype of SNP site to be measured expresses target gene is to make target gene
Expression is lowered, then recommending active constituent in advance is the expression for promoting target gene to the regulating and controlling effect that above-mentioned target gene is expressed；If
The influence mode that the genotype of SNP site to be measured expresses target gene is to keep target gene normal, does not cause to generate corresponding
Skin problem, in response to this, can not have to individual recommend active constituent, it is provided by the present invention recommend be suitable for individual
The targeted individual of the method for active constituent refers to the individual there are skin problem.
Pass through the active constituent for having regulating and controlling effect to the expression of above-mentioned target gene for retrieving existing report and accordingly record
Document, search for research of the reported active constituent on different sample types, find its active constituent and target gene table
The evidence of relationship between reaching.Can strongly suggest active constituent to the regulating and controlling effect of target gene so that recommended activity at
Divide more convincing and scientific.
Further, document is retrieved in open database.Open database be selected from by NCBI PubMed, Elsevier,
The group of Springer, Thomson Reuters and China Biomedical Literature Database composition.
In some embodiments of the present invention, active constituent is to pass through NCBI to the search of the report of target gene effect
The database of PubMed carries out document crawler calling, retrieves using skin relevant report as core, on this basis respectively with difference
The related gene of skin problem, ingredient, effect, experimental method etc. is as the foundation further inquired.
Step S3: according to every kind of above-mentioned pre- height descending for recommending the correlation between active constituent and target gene expression
Arrangement, as the recommendation results (or be suggested design or be skin care solution) to above-mentioned individual.
Further, the height of correlation is mainly divided according to above-mentioned research type reported in the literature, above-mentioned to grind
The level for studying carefully type is higher, and correlation is higher.
The level height for studying type arranges successively in descending order are as follows: human colony's research, mankind human research, animal are ground
Study carefully, human cells in vitro's research, animal cell in vitro is studied and external biochemical test research.The level of human colony's research is most
It is high, be then successively mankind human research, zooscopy, human cells in vitro's research, animal cell in vitro research and external
Biochemical test research, wherein the level of external biochemical test research is minimum, as shown in Figure 1.
Oxford University evidence-based medicine EBM EBM (Evidence Based Medicine) is the research to different medical clinic
Grade assessment system, core concept are that medical decision making (i.e. the processing of patient, treatment guidelines and medical policy making etc.) is answered
Existing best clinical research according on the basis of make.Carry out system is reported in this research by existing different level
Property assessment mode be widely used in human health nursing every field, and skin care item active constituent for Skin Cell very
To the improvement and curative effect of entire body, belong to the peripheral level of human health improvement, but for active constituent to gene
Different level influence research but never carried out systematicness assessment.
The present inventor uses for reference the assessment system and thought of part EBM, for the first time by different pre- recommendation active constituents
The laboratory and clinical research influence on target gene carries out grade classification and assessment (i.e. correlation height divides), innovatively
Recommend the optimal active constituent for individual.The level of difference research is the evidence pyramid mould based on MEDLINE database
Type, according to the correlation of every kind of research and human clinical, to classify from top to bottom.Level is higher, and corresponding data are said
Bright property is stronger, more preferential recommended.In this way, the active constituent recommended is also the most effective work for being directed to target gene variation
Property ingredient.
That is, the pre- sample type for recommending active constituent to study with target gene relationship between expression reported in the literature is
Human colony then illustrates that the correlation between the pre- recommendation active constituent and target gene expression is also higher.Prior to sample
Type is the pre- recommendation active constituent mutually individual recommendation of mankind's human body.
Need be to correlation height partition description: if two kinds of pre- research classes for recommending active constituents that there is same level
Type, the height of correlation then according to corresponding document (with it is identical research type research level) in record pre- recommendation activity at
The influence percentage variation height expressed target gene is divided to divide, percentage variation is higher, and correlation is higher；If single
It is pre- that active constituent is recommended to be corresponding with the influence percentage that multiple document reports express target gene, then the pre- recommendation it is active at
Divide and multiple documents (the research level with identical research type) report is taken to the influence percentage variation that target gene is expressed
Average value as correlation height partitioning standards；If two kinds of pre- influence percentages for recommending active constituent to express target gene
It is more consistent than changing, then compare this two kinds pre- influences for recommending active constituent in lower-level research type to target gene expression
Percentage variation height, percentage variation is higher, and correlation is higher.
After to the pre- height sequence for recommending the correlation between active constituent and target gene expression, as recommendation side
Case is recommended to individual, and such suggested design has stronger specific aim, systematicness and science.
On the other hand, it the present invention also provides a kind of method of skin care item for recommending to be suitable for individual, is pushed away according to above-mentioned
It recommends and recommends the skin care item containing the active constituent to individual suitable for the obtained recommendation results of method of the active constituent of individual.
In another aspect, the present invention also provides a kind of skin care method, according to above-mentioned recommendation be suitable for the activity of individual at
The obtained recommendation results of method divided contact the skin of the individual using the skin care item containing the active constituent.
Feature and performance of the invention are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment by there is a problem of skin complexion is more black, need the individual of whitening for, pushed away to provided by the invention
It recommends and is illustrated suitable for the method recommendation of the active constituent of individual.
The method of the active constituent provided in this embodiment for recommending to be suitable for individual comprising following steps:
1.1 detect the genotype of SNP site, that is, rs10765198 to be measured on target gene, that is, TYR gene of the individual.
Subsequent step is carried out if being C if the genotype in the site rs10765198, if the genotype in the site rs10765198 is that T does not have to push away
It recommends.In the present embodiment, the site rs10765198 is C on the TYR gene of the individual.
Wherein, TYR gene information is shown in Table 1.
Table 1
TYR gene, that is, tyrosinase cdna, the generation of melanin polymer are considered pertaining only to a cuprase, referred to as junket
Propylhomoserin enzyme (TYR), the first step of tyrosinase catalysis melanin access key, i.e. tyrosine hydroxyl turn to DOPA；It is also assisted in
The reactions of two subsequent steps.Contain from the apparent melanin that goes out as the result is shown of the melanocyte of different type Skins culture
Correlation between amount and tyrosinase activity.Because the encoding gene of tyrosine is different in different races, Hei Renpi
Skin tyrosinase activity is 10 times of white man's skin.
The genotype in the site rs10765198 is shown in Table 2 to the result information of the influence mode of TYR gene expression.
Table 2
For carrying the individual of C nucleotide on the site rs10765198, TYR enzyme expression, which can be better than, carries T nucleotide
Individual, lead to the function of stronger TYR enzyme, cause the generation of more melanin, the cutaneous colour of skin leads to skin
Black problem.
1.2 retrievals are it has been reported that active constituent has the document of regulating and controlling effect to the expression of TYP gene, in work reported in the literature
Pre- recommendation active constituent is selected in property ingredient type.Wherein, recommend in advance active constituent to the regulating and controlling effect of TYP gene expression with
Influence mode that above-mentioned target gene express in the site rs10765198 that said gene type is C on the contrary, i.e. it is pre- recommend it is active at
Divide and inhibits TYP gene expression.
In the present embodiment, document crawler calling is carried out by the database in NCBI PubMed, retrieval is with skin correlation
It is reported as core, on this basis respectively with the related gene of the black problem of skin (TYP gene), ingredient, effect, experimental method etc.
As the foundation further inquired.Whitening active ingredients are more as the result is shown.It therefrom selects and inhibiting effect is generated to TYR gene
Active constituent alpha-arbutin, four victory peptide PKEK, L-AA recommends active constituent as pre-.
In the present embodiment, from report alpha-arbutin, four victory peptide PKEK in all Research Literatures of L-AA, are mentioned
The research type (i.e. sample type) to each active constituent is taken out, to the percentage information of TYP gene inhibiting effect, gene regulation
Mode, effective concentration etc., is specifically shown in Table 3.
Table 3
Note: INCI: Intemational Nomenclature cosmetic material ,+: it indicates there are the research of this type, ↓: indicate that expression inhibiting is made
With.
1.3 arrange according to every kind of pre- height descending for recommending the correlation between active constituent and target gene expression, make
For to individual recommendation results.The height of correlation is mainly divided according to research type reported in the literature, and type is studied
Level it is higher, correlation is higher.
The level height for studying type arranges in descending order are as follows: human colony's research, mankind human research, zooscopy, body
Outer human body cell research, animal cell in vitro research and external biochemical test research.Human colony research level highest, according to
Secondary is that mankind human research, zooscopy, human cells in vitro's research, animal cell in vitro research and external biochemical test are ground
Study carefully, the level of external biochemical test research is minimum, as shown in Figure 1.
If two kinds of pre- research types for recommending active constituent to have same level, the height of correlation is according to above-mentioned document
The pre- recommendation active constituent of middle record changes height to the influence percentage that target gene is expressed to divide, and percentage variation is got over
Height, correlation are higher；If two kinds of pre- influence percentage variations for recommending active constituent to express target gene are consistent, compare this
Two kinds of pre- influence percentage variation height for recommending active constituent to express in lower-level research type target gene, percentage
Higher than changing, correlation is higher；If the single pre- shadow for recommending active constituent to there are multiple document reports to express target gene
Percentage is rung, then the pre- recommendation active constituent is averaged as correlation the influence percentage variation that target gene is expressed
The partitioning standards of height.
Therefore, it is divided in conjunction with the correlation of the information of table 3 and step 1.3 it is found that four victory peptide PKEK have in human colony's layer
Its inhibiting effect to TYR gene is proved in the research type of grade, and the research type of alpha-arbutin and L-AA is
In human cells in vitro's level and external biochemical test level, therefore, four victory peptide PKEK are by preferential recommendation.
Compare alpha-arbutin and L-AA, in vitro human body cell level its inhibiting effect of gene (is influenced
Percentage variation) close (being all 40%), it is right in this lower-level on biochemical test in vitro to compare the two active constituents
The inhibiting effect of gene.By calculating the average value of two kinds of ingredients report, alpha-arbutin is (40%+ to the inhibiting effect of TYR
80%)/2=60%；L-AA is about (90%+70% /) 2=80% to the inhibiting effect of TYR.L-AA is from same
The comparison of one level experimental result, L-AA is by preferential recommendation.
The obtained recommendation results to the present embodiment individual are as follows: 1. 4 victory peptide PKEK；2.L- ascorbic acid；3. α-black bearberry
Glycosides.
Embodiment 2
The present embodiment by there are the skin problem of skin aging and wrinkle, need anti-aging, anti-wrinkle individual for, it is right
The method recommendation provided by the invention for recommending the active constituent for being suitable for individual is illustrated.
The method of the active constituent provided in this embodiment for recommending to be suitable for individual comprising following steps:
2.1 detect the genotype of SNP site, that is, rs1799750 to be measured on target gene, that is, MMP1 gene of the individual.
If the site rs1799750 is the insertion mutation for having G nucleotide, subsequent step is carried out, if the site rs1799750 is that G does not occur
The insertion mutation of nucleotide does not have to then carry out subsequent step.In the present embodiment, rs1799750 on the MMP1 gene of the individual
Point has the insertion mutation of G nucleotide.
Wherein, MMP1 gene information is shown in Table 4.
Table 4
MMP1 gene encodes Fibroblast collagenase, and the excessive damage for skin collagen matrix is that skin light decay is old
One major reason raises quilt by throughout the year chronic or randomness the expression for being exposed to the MMP1 gene caused by solar ultraviolet
The main reason for being considered collagen destruction or degradation.MMP1 is a main collagen degradation enzyme, it will lead to serious
The old problem of skin light decay
The genotype in the site rs1799750 is shown in Table 5 to the result information of the influence mode of MMP1 gene expression.
Table 5
During skin aging and wrinkle generate, rs1799750 is on Fibroblast collagenase gene (MMP1)
Site, its diversity, which has been reported, has very strong correlation (PMID:9850057) for the aging of skin and wrinkle.
The individual of G nucleotide insertion mutation, MMP1 are wherein carried for the site rs1799750 on MMP1 gene promoter
Expression can be better than the individual for not carrying this nucleotide, lead to the function of stronger Fibroblast collagenase, cause more
The degradation of collagen leads to the skin problems such as skin aging and wrinkle.
2.2 retrievals are it has been reported that active constituent has the document of regulating and controlling effect to the expression of MMP1 gene, reported in the literature
Pre- recommendation active constituent is selected in active constituent type.Wherein, active constituent is recommended to make the regulation of MMP1 gene expression in advance
With with there is the site rs10765198 of G nucleotide insertion mutation to express target gene influence mode on the contrary, i.e. pre- recommend to live
Property ingredient inhibit MMP1 gene expression.
In the present embodiment, find that the active constituent of anti-aging is more, therefrom selects to MMP1 gene table by retrieval
Recommend to live as pre- up to the active constituent Vitamin A for generating inhibiting effect, Co-Q10, marine algae extract and extract of soybean
Property ingredient.These active constituents have inhibiting effect (being shown in Table 6) to MMP1 gene.
In the present embodiment, from report Vitamin A, Co-Q10, all researchs of marine algae extract and extract of soybean
In document, the research type (i.e. sample type) to each active constituent is extracted, the percentage of MMP1 gene inhibiting effect is believed
Breath, gene regulation mode, effective concentration etc. are specifically shown in Table 6.
Table 6
Note: INCI: Intemational Nomenclature cosmetic material ,+: it indicates there are the research of this type, ↓: indicate that expression inhibiting is made
With.
2.3 arrange according to every kind of pre- height descending for recommending the correlation between active constituent and target gene expression, make
For to individual recommendation results.The height of correlation is mainly divided according to research type reported in the literature, and type is studied
Level it is higher, correlation is higher.
The level height for studying type arranges in descending order are as follows: human colony's research, mankind human research, zooscopy, body
Outer human body cell research, animal cell in vitro research and external biochemical test research.Human colony research level highest, according to
Secondary is that mankind human research, zooscopy, human cells in vitro's research, animal cell in vitro research and external biochemical test are ground
Study carefully, the level of external biochemical test research is minimum, as shown in Figure 1.
If two kinds of pre- research types for recommending active constituent to have same level, the height of correlation is according to above-mentioned document
The pre- recommendation active constituent of middle record changes height to the influence percentage that target gene is expressed to divide, and percentage variation is got over
Height, correlation are higher；If two kinds of pre- influence percentage variations for recommending active constituent to express target gene are consistent, compare this
Two kinds of pre- influence percentage variation height for recommending active constituent to express in lower-level research type target gene, percentage
Higher than changing, correlation is higher；If the single pre- shadow for recommending active constituent to there are multiple document reports to express target gene
Percentage is rung, then the pre- recommendation active constituent is averaged as correlation the influence percentage variation that target gene is expressed
The partitioning standards of height.
Therefore, it divides in conjunction with the correlation of the information of table 3 and step 2.3 it is found that Vitamin A has in human colony
Prove its inhibiting effect to MMP1 gene, and other reports of three kinds of ingredients only on human body cell, therefore Vitamin A
By preferential recommendation.Comparing marine algae extract, extract of soybean and Co-Q10, it makees the inhibition of gene in human body cell level
With.By calculating the average value of every kind of ingredient report, inhibiting effect (75%+50%)/2=of the marine algae extract to MMP1
62.5%, extract of soybean is about (50%+60%)/2=55% to the inhibiting effect of MMP1.Inhibition of the Co-Q10 to MMP1
Effect is about (35%+40%)/2=37.5%.From the comparison of same level experimental result, recommendation order is successively are as follows: seaweed mentions
Take object, extract of soybean and Co-Q10.
The obtained suggested design to the present embodiment individual are as follows: 1.Vitamin A；2. marine algae extract；3. soybean is extracted
Object；4. Co-Q10.
Embodiment 3
The present embodiment by there are the skin problem of skin oxidative, need it is oxidation resistant individual for, to provided by the invention
The method recommendation for the active constituent for being suitable for individual is recommended to be illustrated.
The method of the active constituent provided in this embodiment for recommending to be suitable for individual comprising following steps:
3.1 detect the genotype of SNP site, that is, rs2070424 to be measured on target gene, that is, SOD1 gene of the individual.
If the site rs2070424 is the insertion mutation for having A nucleotide, subsequent step is carried out, if the site rs2070424 is that A does not occur
The insertion mutation of nucleotide does not have to then carry out subsequent step.In the present embodiment, rs2070424 on the SOD1 gene of the individual
Point has the insertion mutation of A nucleotide.
Wherein, the relevant information of SOD1 gene is shown in Table 7.
Table 7
SOD1 gene encoding superoxide dismutase eliminates the harmful substance that organism generates in metabolic processes.
It is the antioxidant enzyme for protecting the skin from peroxide pressure important in body.It is for the super oxygen in scavenger-cell
Object ion has played important function, these superoxide ions are the active oxidation substances (PMID:20352474) generated into the cell.
The genotype in the site rs2070424 is shown in Table 8 to the result information of the influence mode of SOD1 gene expression.
Table 8
During skin oxidative, rs2070424 is the site to be measured on superoxide dismutase SOD1 gene, it more
Sample, which has been reported, has very deep influence (PMID:20352474) for the oxidation resistance of individual's skin.Wherein for SOD1
SNP site (rs2070424) carries the individual of A nucleotides inserted mutation on gene, and the expression of SOD1, which is weaker than, carries this core of A
The individual of thuja acid leads to the reduction of superoxide dismutase, final to weaken the oxidation resistant ability of skin (PMID:22972774),
Skin is caused to generate the skin problems such as aging and wrinkle.
3.2 retrievals are it has been reported that active constituent has the document of regulating and controlling effect to the expression of SOD1 gene, reported in the literature
Pre- recommendation active constituent is selected in active constituent type.Wherein, active constituent is recommended to make the regulation of SOD1 gene expression in advance
With influence mode express with the site rs10765198 for thering is A nucleotides inserted to be mutated to target gene on the contrary, i.e. pre- recommendation work
1 gene expression of property ingredient enhancement of SOD.
In the present embodiment, find that oxidation resistant active constituent is more, therefrom selects to SOD1 gene table by retrieval
Up to active constituent Vitamin E, the Vitamin C for generating humidification, green-tea extract and resveratrol as with recommend at
Point.These active constituents have humidification (being shown in Table 9) to SOD1 gene.
In the present embodiment, from report Vitamin E, Vitamin C, all researchs of green-tea extract and resveratrol
In document, the research type (i.e. sample type) to each active constituent is extracted, the percentage of SOD1 genes amplification effect is believed
Breath, gene regulation mode, effective concentration etc. are specifically shown in Table 9.
Table 9
Note: INCI: Intemational Nomenclature cosmetic material ,+: it indicates there are the research of this type, ↑: indicate that expression enhancing is made
With.
3.3 arrange according to every kind of pre- height descending for recommending the correlation between active constituent and target gene expression, make
For to individual recommendation results.The height of correlation is mainly divided according to research type reported in the literature, and type is studied
Level it is higher, correlation is higher.
The level height for studying type arranges in descending order are as follows: human colony's research, mankind human research, zooscopy, body
Outer human body cell research, animal cell in vitro research and external biochemical test research.Human colony research level highest, according to
Secondary is that mankind human research, zooscopy, human cells in vitro's research, animal cell in vitro research and external biochemical test are ground
Study carefully, the level of external biochemical test research is minimum.
If two kinds of pre- research types for recommending active constituent to have same level, the height of correlation is according to above-mentioned document
The pre- recommendation active constituent of middle record changes height to the influence percentage that target gene is expressed to divide, and percentage variation is got over
Height, correlation are higher；If two kinds of pre- influence percentage variations for recommending active constituent to express target gene are consistent, compare this
Two kinds of pre- influence percentage variation height for recommending active constituent to express in lower-level research type target gene, percentage
Higher than changing, correlation is higher；If the single pre- shadow for recommending active constituent to there are multiple document reports to express target gene
Percentage is rung, then the pre- recommendation active constituent is averaged as correlation the influence percentage variation that target gene is expressed
The partitioning standards of height.
Therefore, it divides in conjunction with the correlation of the information of table 9 and step 3.3 it is found that Vitamin E, green-tea extract and white
Veratryl alcohol all proves its humidification to SOD1 gene in animal individual, by calling them to express facilitation to SOD1
Average value, Vitamin E is (10%+30%)/2=20% to the humidification of SOD1 gene, and green-tea extract is to SOD1
The humidification of gene is 100%, and resveratrol is 60% to the humidification of SOD1 gene.So preferential recommendation green tea extracts
Object is later resveratrol, Vitamin E.Since Vitamin C is only proved to be able to enhancement of SOD 1 on zooblast in vitro
Expression (100%), Vitamin C finally recommended.
Obtain to individual recommendation results are as follows: 1. green-tea extracts；2. resveratrol；3.Vitamin E；4.Vitamin
C。
Embodiment 4
A kind of method for present embodiments providing skin care item for recommending to be suitable for individual, this method are provided according to embodiment 1
The obtained recommendation results of method for being suitable for the active constituent of individual are recommended to recommend the skin care item containing active constituent to individual.I.e.
The present embodiment is prioritized successively to individual recommendation results described in embodiment 1 are as follows: 1. contain the shield of four victory peptide PKEK
Skin product, 2. skin care item, 3. skin care item containing alpha-arbutin containing L-AA.
Embodiment 5
A kind of skin care method is present embodiments provided, the active constituent of individual is suitable for according to the recommendation that embodiment 1 provides
The obtained recommendation results of method contact the skin of individual described in embodiment 1 using the skin care item containing active constituent.
It is i.e. preferential to contact skin using the skin care item containing four victory peptide PKEK, to improve the black skin problem of skin.
To sum up, it is provided by the invention recommend be suitable for individual active constituent method, this method based on individual and skin
On the relevant target gene of feature (such as TYP gene, MMP1 gene, SOD1 gene) SNP site to be measured (such as
Rs10765198, rs1799750, rs2070424) genotype, obtain the influence mode expressed target gene of genotype, really
The inherent genetic cause for having determined generation skin problem is selected according in the i.e. disclosed document report of existing research evidence to target
The pre- recommendation active constituent that the influence mode that the regulating and controlling effect genotype of gene expression expresses target gene is sent out on the contrary, according to choosing
As the recommendation to individual after the height descending arrangement of correlation between pre- recommendation active constituent out and target gene expression
As a result.Inherent genetic cause of the active constituent recommended for individual's skin problem, targeting with higher, and it is selected
Pre- recommendation active constituent and target gene expression between correlation it is high, obtained the strong support and explanation of existing literature,
The same systematicness with higher of the recommendation results and science.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Industrial applicibility: the method for the active constituent provided by the invention for recommending to be suitable for individual, this method is based on individual
The genotype of SNP site to be measured on target gene, obtains influence mode express target gene of genotype, target gene and
Skin characteristic is related, determines the inherent genetic cause for generating skin problem, further according to the i.e. disclosed document of existing research evidence
It is selected in report and supplement work is generated to the influence mode that target gene is expressed to the regulating and controlling effect and genotype of target gene expression
Pre- recommendation active constituent, according to the high sinking of the correlation between the pre- recommendation active constituent selected and target gene expression
As the recommendation results to individual after sequence arrangement.The active constituent recommended is directed to the inherent genetic cause of individual's skin problem,
Targeting with higher, and the correlation between selected pre- recommendation active constituent and target gene expression is high, obtains
The strong support and explanation of existing literature, recommendation results systematicness with higher and science.
Claims (10)
1. a kind of method for the active constituent for recommending to be suitable for individual, the active constituent are configured to the primary raw material of skin care item,
It is characterized in that comprising:
The genotype for detecting the SNP site to be measured on the target gene of individual, obtains the genotype to the target gene table
The influence mode reached, wherein the target gene is related to skin characteristic；
Retrieval is it has been reported that active constituent has the document of regulating and controlling effect to the expression of the target gene, described reported in the literature
Pre- recommendation active constituent, the pre- regulation for recommending active constituent to express the target gene are selected in active constituent type
Effect generates supplementary function to the influence mode that the target gene is expressed with the genotype；
It is arranged, is made according to the pre- height descending for recommending the correlation between active constituent and target gene expression described in every kind
For to the recommendation results of the individual.
2. the method for the active constituent according to claim 1 for recommending to be suitable for individual, which is characterized in that the height of correlation
It is divided according to the research type reported in the literature, the level of the research type is higher, and correlation is higher.
3. the method for the active constituent according to claim 2 for recommending to be suitable for individual, which is characterized in that the research type
Level height arrange in descending order successively are as follows: human colony's research, mankind human research, zooscopy, human cells in vitro grind
Study carefully, animal cell in vitro research and external biochemical test are studied.
4. the method for the active constituent according to claim 3 for recommending to be suitable for individual, which is characterized in that have same level
Research type, the height of correlation is then according to the pre- recommendation active constituent recorded in the document to the target gene
The influence percentage variation of expression just divides, and percentage variation is higher, and correlation is higher.
5. the method for the active constituent according to claim 1-4 for recommending to be suitable for individual, which is characterized in that described
Skin characteristic includes one or more of black, aging, oxidation, wrinkle, acne.
6. the method for the active constituent according to any one of claims 1-5 for recommending to be suitable for individual, which is characterized in that institute
It states target gene and is selected from one of TYR gene, MMP1 gene and SOD1 gene；
SNP site to be measured on the TYR gene is rs10765198, and the SNP site to be measured on the MMP1 gene is
Rs1799750, the SNP site to be measured on the SOD1 gene are rs2070424.
7. the method for the active constituent according to claim 1-6 for recommending to be suitable for individual, which is characterized in that opening
It puts and retrieves the document in database.
8. the method for the active constituent according to claim 7 for recommending to be suitable for individual, which is characterized in that the open data
Library is selected from by NCBI PubMed, Elsevier, Springer, Thomson Reuters and Chinese biomedical literature data
The group of library composition.
9. a kind of method for the skin care item for recommending to be suitable for individual, which is characterized in that it is according to claim 1 described in any one of -8
Recommendation be suitable for individual active constituent the obtained recommendation results of method to individual recommend contain the active constituent shield
Skin product.
10. a kind of skin care method, which is characterized in that its recommendation according to claim 1 to 8 is suitable for individual
The obtained recommendation results of the method for active constituent contact the skin of the individual using the skin care item containing the active constituent.
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Application publication date: 20181214 |