CN108991522A - A kind of preparation method of high load curcumin tea oil O/W nanoemulsions - Google Patents
A kind of preparation method of high load curcumin tea oil O/W nanoemulsions Download PDFInfo
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- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 title claims abstract description 134
- 235000012754 curcumin Nutrition 0.000 title claims abstract description 67
- 229940109262 curcumin Drugs 0.000 title claims abstract description 67
- 239000004148 curcumin Substances 0.000 title claims abstract description 67
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 title claims abstract description 67
- 239000010495 camellia oil Substances 0.000 title claims abstract description 52
- 239000007908 nanoemulsion Substances 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 238000003756 stirring Methods 0.000 claims abstract description 50
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000004094 surface-active agent Substances 0.000 claims abstract description 15
- 239000002131 composite material Substances 0.000 claims abstract description 13
- 229960000935 dehydrated alcohol Drugs 0.000 claims abstract description 9
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 8
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 8
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 8
- 239000012498 ultrapure water Substances 0.000 claims abstract description 8
- 238000005303 weighing Methods 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 7
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 230000003647 oxidation Effects 0.000 abstract description 3
- 238000007254 oxidation reaction Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 230000014759 maintenance of location Effects 0.000 description 15
- 239000000243 solution Substances 0.000 description 10
- 239000006210 lotion Substances 0.000 description 8
- 239000003963 antioxidant agent Substances 0.000 description 6
- 235000006708 antioxidants Nutrition 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 230000003078 antioxidant effect Effects 0.000 description 5
- 238000009826 distribution Methods 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 230000031700 light absorption Effects 0.000 description 3
- 150000003254 radicals Chemical class 0.000 description 3
- 244000163122 Curcuma domestica Species 0.000 description 2
- 235000003392 Curcuma domestica Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 235000003373 curcuma longa Nutrition 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 229960004756 ethanol Drugs 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 235000013976 turmeric Nutrition 0.000 description 2
- KKAJSJJFBSOMGS-UHFFFAOYSA-N 3,6-diamino-10-methylacridinium chloride Chemical compound [Cl-].C1=C(N)C=C2[N+](C)=C(C=C(N)C=C3)C3=CC2=C1 KKAJSJJFBSOMGS-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 241001122767 Theaceae Species 0.000 description 1
- 244000273928 Zingiber officinale Species 0.000 description 1
- 235000006886 Zingiber officinale Nutrition 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000021281 monounsaturated fatty acids Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001603 reducing effect Effects 0.000 description 1
- 235000020195 rice milk Nutrition 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
- A23L33/12—Fatty acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
- A23P10/35—Encapsulation of particles, e.g. foodstuff additives with oils, lipids, monoglycerides or diglycerides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
- A61K8/062—Oil-in-water emulsions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Biophysics (AREA)
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Abstract
The present invention discloses a kind of preparation method of high load curcumin tea oil O/W nanoemulsions, its step are as follows: weighing curcumin and is added to stirring and dissolving in dehydrated alcohol, mixed liquor is obtained, mixed liquor is taken to mix with Tween 80, stirring is protected from light and forms surfactant composite solution；The surfactant composite solution is taken, ultrapure water is added dropwise, is protected from light stirring, forms stable curcumin tea oil O/W nanoemulsions.The present invention can solve tea oil in the processing problems of aqueous systems, improves the functional characteristic of tea oil, improves bioavailability, improve curcumin water solubility, improves the oxidation stability of curcumin environmental stability and tea oil.The present invention has safe operation, and effect is good, feature at low cost, has biggish economic advantages.
Description
Technical field
The invention belongs to food processing technology fields, are related to a kind of preparation of high load curcumin tea oil O/W nanoemulsions
Method.
Background technique
Tea oil unsaturated fatty acid rich in, wherein the content of monounsaturated fatty acids is up to 68~77%, how unsaturated
The content of fatty acid is 7~14%, and there is also a large amount of sterols, polyphenols and lipid accompaniments etc. pair in tea oil
The beneficial substance of human body, therefore, tea oil nutrition and health care function with higher can provide necessary nutrient, also
It is of great significance in terms of preventing and treating the functions such as hypertension, cardiovascular disease.
Tea oil is mostly liquid, have high hydrophobic effect, to directly drink or in terms of food, cosmetic applications process,
Using bringing inconvenience.Existing market tea oil product is mostly emulsion, ointment etc., is on the one hand difficult to meet wanting for food processing
It asks, also reduces tea oil bioavailability.Nanoemulsions system is that oily phase, water phase, surfactant mix in the proper ratio
The transparent or semitransparent thermodynamic stable system of one kind of formation, can be used as the carrier of hydrophobic drug, poorly water soluble drugs, tea
The water solubility and bioavailability of tea oil can be improved in oily nanometer system.
Curcumin has anti-inflammatory, anti-oxidant, reducing blood lipid, anti-artery as a kind of common high hydrophobic function plant polyphenol
The multiple functions such as atherosis, antitumor.But as functional component and effective therapeutic agent, the water solubility of curcumin is lower,
The environmental sensibilities such as temperature, ultraviolet light are higher, seriously limit its extensive use.
Summary of the invention
To solve the above problems, the present invention provides a kind of preparation methods of high load curcumin tea oil O/W nanoemulsions.
Heretofore described high load is high-dissolvability.
A kind of preparation method of high load curcumin tea oil O/W nanoemulsions, comprising the following steps:
Step 1: weighing curcumin and be added in dehydrated alcohol, be protected from light stirring, obtain mixed liquor；
Step 2: taking the resulting mixed liquor of step 1 to mix with Tween 80, be protected from light stirring, form surfactant composite solution；
Step 3: tea oil is added dropwise into the resulting surfactant composite solution of step 2, stirs and evenly mixs, obtains mixture
System；
Step 4: ultrapure water is added dropwise into the resulting mixed system of step 3, stirs, mixing speed is 50~600 revs/min
Clock, mixing time are 2~5h.
Further, the mass ratio of curcumin described in step 1 and dehydrated alcohol is (0.1~2): 100.
Further, temperature when stirring in step 1 is 25 DEG C, and mixing speed is 40~600 revs/min, mixing time
For 20~30min.
Further, the mass ratio of mixed liquor described in step 2 and Tween 80 is 1:(1~5).
Further, temperature when stirring in step 2 is 25 DEG C, and mixing speed is 500~1000 revs/min, when stirring
Between be 3~7h.
Further, the mass ratio of tea oil described in step 3 and surfactant composite solution is 1:(2~8).
Further, temperature when stirring in step 3 is 25 DEG C, and mixing speed is 500~1000 revs/min, when stirring
Between be 3~5h.
Further, the mass ratio of ultrapure water described in step 4 and mixed system is (5~7): 10.
Compared with prior art, the beneficial effects of the present invention are:
(1) it solves tea oil in the processing problems of aqueous systems, provides basis in food and cosmetics processing for it；
(2) functional characteristic for improving tea oil, realizes curcumin and the dual physiological function of tea oil；
(3) curcumin water solubility is greatly improved, solubility of the curcumin in pure water is 11 ng/ml, passes through nano-complex
The means such as containing also can be improved its solubility, but solubilizing effect is unobvious, and the present invention can reach 17 mg/mL, and solubility can be improved
Up to ten thousand times, its bioavailability can be greatlyd improve；
(4) present invention improves the oxidation stability of curcumin environmental stability and tea oil, a large number of studies show that curcumin high temperature,
It can degrade and lose activity under the conditions of ultraviolet light etc., method commonly used at present is that some antioxidants are added, and reduces its oxygen
Change characteristic, the means that the present invention is contained by tea oil nano-solution can reduce sensibility of the curcumin element in high temperature, storage rate
About 10%(can be improved and see Fig. 3)；Curcumin is played in the anti-oxidation function of emulsion interface simultaneously, is effectively prevent in tea oil unsaturated
The oxidative rancidity behavior of fatty acid improves the oxidation stability of fatty acid in tea oil.
Detailed description of the invention
Fig. 1 is different curcumin tea oil O/W nanoemulsions system particle diameter distributions, and illustration is the curcumin tea oil of preparation in figure
The outside drawing of O/W nanoemulsions.
Fig. 2 is different curcumin tea oil O/W nanoemulsions system Scavenging abilities (a) and total antioxidant capacity (b).
Fig. 3 is curcumin retention rate in the case of different temperatures and difference contain.
Specific embodiment
The following examples are intended to illustrate the invention, but is not used to limit the scope of protection of the present invention.Unless otherwise specified, real
Apply the common sense that technological means used in example is well known to those skilled in the art.
Embodiment 1
A kind of preparation method of high load curcumin tea oil O/W nanoemulsions, comprising the following steps:
Step 1: weighing 0.5g curcumin and be added in 50g dehydrated alcohol, be protected from light stirring and dissolving, obtain mixed liquor, the stirring
When temperature be 25 DEG C, mixing speed be 60 revs/min, mixing time 30min；
Step 2: the mixed liquor 1g for taking step 1 to obtain is mixed with 3g Tween 80, is protected from light stirring, and temperature when stirring is 25 DEG C, stirs
Mixing speed is 600 revs/min, mixing time 3h, forms surfactant composite solution；
Step 3: with 30 drops/min drop speed, 2g tea oil is added dropwise into step 2 gained surfactant composite solution, stirs
It mixes, obtains mixed system, temperature when stirring is 25 DEG C, mixing speed is 500 revs/min, mixing time 4h；
Step 4: with 30 drops/min drop speed, 4g ultrapure water is added dropwise into step 3 gained mixed system, stirs, stirring speed
Degree is 600 revs/min, mixing time 2h, must load ‰ curcumin tea oil O/W nanoemulsions of 1.0wt, ‰ turmeric of 1.0wt
Particle diameter distribution is 554.0 ± 29.2nm in plain tea oil O/W nanoemulsions, sees Fig. 1；‰ curcumin tea oil O/W of gained 1.0wt receives
The DPPH of rice milk liquid, which removes free radical, can reach 97.3 ± 0.7%, see Fig. 2 a；Its total antioxidant capacity can reach 26.98 ± 0.9
T-AOC/mL is shown in Fig. 2 b.
Embodiment 2
A kind of preparation method of high load curcumin tea oil O/W nanoemulsions, comprising the following steps:
Step 1: weighing 0.18g curcumin and be added in 30g dehydrated alcohol, be protected from light stirring and dissolving, obtain mixed liquor, the stirring
When temperature be 25 DEG C, mixing speed be 80 revs/min, mixing time 20min；
Step 2: the mixed liquor 1g for taking step 1 to obtain is mixed with 3g Tween 80, is protected from light stirring, when stirring temperature be 25 DEG C, stirring
Speed is 600 revs/min, mixing time 5h, forms surfactant composite solution；
Step 3: with 30 drops/min drop speed, 1g tea oil is added dropwise into step 2 gained surfactant composite solution, stirs
It mixes, obtains mixed system, temperature is 25 DEG C when stirring, mixing speed is 800 revs/min, mixing time 5h；
Step 4: with 30 drops/min drop speed, 3g ultrapure water is added dropwise into step 3 gained mixed system, stirs, stirring speed
Degree is 90 revs/min, mixing time 3h, must load ‰ curcumin tea oil O/W nanoemulsions of 0.75wt, ‰ ginger of 0.75wt
Particle diameter distribution is 548.3 ± 30.7nm in flavine tea oil O/W nanoemulsions, sees Fig. 1；0.75 wt of gained, ‰ curcumin tea oil O/
The DPPH of W nanoemulsions, which removes free radical, can reach 86.9 ± 3.2%, see Fig. 2 a, and total antioxidant capacity can reach 29.13 ±
0.4 T-AOC/mL is shown in Fig. 2 b.
Embodiment 3
A kind of preparation method of high load curcumin tea oil O/W nanoemulsions, comprising the following steps:
Step 1: it weighs 0.08g curcumin and is added in 10g dehydrated alcohol, be protected from light stirring and dissolving, obtain mixed liquor, temperature when stirring
Degree is 25 DEG C, and mixing speed is 90 revs/min, mixing time 25min；
Step 2: the mixed liquor 1g for taking step 1 to obtain is mixed with 2g Tween 80, is protected from light stirring, and temperature when stirring is 25 DEG C, stirs
Mixing speed is 900 revs/min, mixing time 3.5h, forms surfactant composite solution；
Step 3: with 30 drops/min drop speed, 1g tea oil being added dropwise into step 2 gained surfactant composite solution, stirring is mixed
It is even, obtain mixed system, temperature is 25 DEG C when stirring, and mixing speed is 700 revs/min, mixing time 3.5h；
Step 4: with 30 drops/min drop speed, 2g ultrapure water being added dropwise into step 3 gained mixed system, stirs, mixing speed
It is 70 revs/min, mixing time 3.5h, ‰ curcumin tea oil O/W nanoemulsions of 1.3wt, ‰ curcumin of 1.3wt must be loaded
Particle diameter distribution is 501.5 ± 12.1nm in tea oil O/W nanoemulsions, sees Fig. 1；O/W nanometers of 1.3 wt of gained, ‰ curcumin tea oil
The DPPH of lotion, which removes free radical, can reach 84.8 ± 1.3%, see Fig. 2 a, total antioxidant capacity can reach 29.13 ± 0.1 T-
AOC/mL is shown in Fig. 2 b.
Embodiment 4
Curcumin retention rate compares in the case of different temperatures and difference contain, and experimental procedure is as follows:
Step 1: preparing curcumin tea oil O/W nanoemulsions using 1 the method for embodiment, weigh the 5g curcumin of preparation respectively
Tea oil O/W nanoemulsions are in 30 brown reagent bottles；
Step 2: weighing 2.55g curcumin and be added in 150g dehydrated alcohol, be protected from light stirring and dissolving, obtain the mixing of curcumin ethyl alcohol
Liquid, the temperature when stirring are 25 DEG C, and mixing speed is 60 revs/min, and mixing time 30min weighs 5g turmeric respectively
Plain alcohol mixeding liquid is in 30 brown reagent bottles；
Step 3: 30 bottles of curcumin ethyl alcohol that the 30 bottles of curcumin tea oil O/W nanoemulsions and step 2 that step 1 obtains obtain are mixed
Close liquid takes 15 bottles to be placed under the conditions of 25 DEG C and 50 DEG C respectively；
Step 4: taking 3 bottles of curcumin tea oil O/W nanoemulsions and 3 respectively in different time sections (0h, 1h, 3h, 7h, 21h, 29h)
Bottle curcumin alcohol mixeding liquid after 1500 times of dilution, measures light absorption value in 428nm；
Step 5: calculate curcumin retention rate, retention rate (%)=At × 100/A0, light absorption value when wherein At refers to any time period,
Light absorption value when A0 is initial.
The results show that the curcumin retention rate under the conditions of curcumin retention rate is apparently higher than 50 DEG C under the conditions of 25 DEG C.In 7h
When, lotion contains curcumin under the conditions of 25 DEG C and unentrapped curcumin retention rate difference reaches maximum.When 21h, under the conditions of 50 DEG C
Lotion contains curcumin and unentrapped curcumin retention rate difference reaches maximum.When 29h, lotion contains curcumin under the conditions of 25 DEG C
It is dropped to unentrapped curcumin retention rate minimum, wherein unentrapped curcumin retention rate contains curcumin guarantor slightly larger than lotion
Stay rate；Under the conditions of 50 DEG C lotion contain curcumin and unentrapped curcumin retention rate decline also drop to it is minimum, wherein lotion packet
It carries curcumin retention rate and is slightly larger than unentrapped curcumin retention rate.After 7h, the decline of curcumin retention rate becomes under the conditions of 50 DEG C
Curcumin retention rate (see figure 3) under the conditions of gesture is all larger than 25 DEG C.The lotion, which contains, refers to that tea oil O/W nanoemulsions contain.
The embodiment of the above, only presently preferred embodiments of the present invention, is only used to explain the present invention, not limit
The scope of the present invention processed to those of ordinary skill in the art certainly can be according to skill disclosed in this specification
Art content, makes other embodiments easily by way of replacing or changing, therefore all in the principle of the present invention and technique item
The changes and improvements etc. that part is done, should be included in scope of the present invention patent.
Claims (8)
1. a kind of preparation method of high load curcumin tea oil O/W nanoemulsions, which comprises the following steps:
Step 1: weighing curcumin and be added in dehydrated alcohol, be protected from light stirring, obtain mixed liquor；
Step 2: taking the resulting mixed liquor of step 1 to mix with Tween 80, be protected from light stirring, form surfactant composite solution；
Step 3: tea oil is added dropwise into the resulting surfactant composite solution of step 2, stirs and evenly mixs, obtains mixture
System；
Step 4: ultrapure water is added dropwise into the resulting mixed system of step 3, stirs, mixing speed is 50~600 revs/min
Clock, mixing time are 2~5h.
2. preparation method according to claim 1, which is characterized in that the matter of curcumin described in step 1 and dehydrated alcohol
Amount is than being (0.1~2): 100.
3. preparation method according to claim 1, which is characterized in that temperature when stirring in step 1 is 25 DEG C, stirring speed
Degree is 40~600 revs/min, and mixing time is 20~30min.
4. preparation method according to claim 1, which is characterized in that the quality of mixed liquor described in step 2 and Tween 80
Than for 1:(1~5).
5. preparation method according to claim 1, which is characterized in that temperature when stirring in step 2 is 25 DEG C, stirring speed
Degree is 500~1000 revs/min, and mixing time is 3~7h.
6. preparation method according to claim 1, which is characterized in that tea oil described in step 3 and surfactant are compound
The mass ratio of solution is 1:(2~8).
7. preparation method according to claim 1, which is characterized in that temperature when stirring in step 3 is 25 DEG C, stirring speed
Degree is 500~1000 revs/min, and mixing time is 3~5h.
8. preparation method according to claim 1, which is characterized in that the matter of ultrapure water described in step 4 and mixed system
Amount is than being (5~7): 10.
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| CN201810645041.4A CN108991522A (en) | 2018-06-21 | 2018-06-21 | A kind of preparation method of high load curcumin tea oil O/W nanoemulsions |
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| CN201810645041.4A CN108991522A (en) | 2018-06-21 | 2018-06-21 | A kind of preparation method of high load curcumin tea oil O/W nanoemulsions |
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Citations (4)
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| CN1736369A (en) * | 2004-08-12 | 2006-02-22 | 山东绿叶天然药物研究开发有限公司 | Curcumin emulsion, its preparation process and use |
| CN103784421A (en) * | 2014-02-27 | 2014-05-14 | 哈尔滨医科大学 | Curcumin and piperine carried solid lipid nanoparticles and preparation method thereof |
| US20170000704A1 (en) * | 2015-06-30 | 2017-01-05 | The Gillette Company | Personal care compositions comprising a sensate |
| CN106727316A (en) * | 2017-02-14 | 2017-05-31 | 福州大学 | A kind of curcumin betadex nano-emulsion agent and preparation method thereof |
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- 2018-06-21 CN CN201810645041.4A patent/CN108991522A/en active Pending
Patent Citations (4)
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|---|---|---|---|---|
| CN1736369A (en) * | 2004-08-12 | 2006-02-22 | 山东绿叶天然药物研究开发有限公司 | Curcumin emulsion, its preparation process and use |
| CN103784421A (en) * | 2014-02-27 | 2014-05-14 | 哈尔滨医科大学 | Curcumin and piperine carried solid lipid nanoparticles and preparation method thereof |
| US20170000704A1 (en) * | 2015-06-30 | 2017-01-05 | The Gillette Company | Personal care compositions comprising a sensate |
| CN106727316A (en) * | 2017-02-14 | 2017-05-31 | 福州大学 | A kind of curcumin betadex nano-emulsion agent and preparation method thereof |
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