CN108976819A - A kind of preparation method of soybean protein isolate-chitosan composite particle - Google Patents
A kind of preparation method of soybean protein isolate-chitosan composite particle Download PDFInfo
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- CN108976819A CN108976819A CN201810777042.4A CN201810777042A CN108976819A CN 108976819 A CN108976819 A CN 108976819A CN 201810777042 A CN201810777042 A CN 201810777042A CN 108976819 A CN108976819 A CN 108976819A
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- soybean protein
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
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Abstract
The present invention provides a kind of preparation methods of soybean protein isolate-chitosan composite particle, the following steps are included: (1) is mixed soybean protein isolate solution with chitosan solution by 2~20:1 volume ratio, its mixed solution is obtained, and adjusts pH value to 4~5；(2) mixed solution of step (1) is heated into 20min under the conditions of temperature is 60 DEG C~100 DEG C；(3) it is centrifuged after mixed solution cooling, takes supernatant to get soybean protein isolate-chitosan composite particle solution.The novel composite particles for having functional characteristic of multiplicity can be formed using preparation method of the present invention.Preparation process of the invention is simple, and has pH responsiveness and ionic strength responsiveness, can be used for the embedding of nutriment, raw material sources are extensive.
Description
Technical field
The invention belongs to modern healthy food processing fields, and in particular to a kind of soybean protein isolate-chitosan is compound to be received
The preparation method of rice grain prepares soybean protein isolate-chitosan composite nanometer particle, the nano particle by the method for heat system
The embedding that can be used for food nutrition substance in future, belongs to modern healthy food processing field.
Background technique
World of Food industrial expansion trend just moves towards predictability from the manufacturability for chasing productivity and optimization product
High-quality, high technology content product research and development and manufacture.In recent ten years, how to effectively control food component interactions to come
The food system of manufacture (or design) with different micro-structures and functional characteristic is always by domestic and international Food Science Research Team
Highest attention.Protein and natural polysaccharide are two kinds of important natural macromolecular materials, have resource abundant and source is wide
It is general, possess a series of excellent performances such as renewable, nontoxic, good biocompatibility and biodegradability.They also because
It is widely used in food, pharmacy and genetic engineering industry for its many functional character.In numerous food product system,
Protein and polysaccharide determine stability, structure, Viscosity Properties, texture property and the taste of product.
Currently, studies in China soybean protein-chitosan compound still predominantly stays in the stage of the two interaction,
And the research for the composite particles for preparing the two by heat mode processed has not been reported.Foreign countries have part to prepare newborn egg using heating method
The compound of the polysaccharide such as white and chitosan, pectin, carragheen, to form the research of composite particles, but has no soybean separation protein
The white combination with chitosan.Comprehensive present Research both domestic and external, has no and prepares soybean protein isolate-chitosan by heat mode
The document and patent report of composite particles.
Summary of the invention
A kind of heat soybean protein isolate-is provided it is an object of the invention to overcome above-mentioned the deficiencies in the prior art place
The preparation method of chitosan composite nanometer particle, prepared nano particle is in the embedding that can be used for food nutrition substance.
To achieve the above object, a kind of the technical solution adopted by the present invention are as follows: soybean protein isolate-chitosan composite particle
Preparation method, comprising the following steps:
(1) soybean protein isolate solution is mixed with chitosan solution by 2~20:1 volume ratio, obtains its mixed solution,
And pH value is adjusted to 4~5；
(2) mixed solution of step (1) is heated into 20min under the conditions of temperature is 60 DEG C~100 DEG C；
(3) it is centrifuged after mixed solution cooling, takes supernatant molten to get soybean protein isolate-chitosan composite particle
Liquid.
Preferably, soybean protein isolate solution is prepared using following methods in the step (1): weighing certain mass
Soybean protein isolate be dissolved in water, be configured to 0.5~2% soybean protein isolate solution.
Preferably, chitosan solution is prepared using following methods in the step (1): by the chitosan of certain mass
The acetic acid buffer solution being dissolved in is configured to 0.1~1% chitosan solution.
Preferably, the concentration of the acetic acid buffer solution is 100mM.
Preferably, the pH value in the step (1) is 4.25,4.55 or 4.9.
Preferably, the heating means in the step (2) are heating water bath.
Preferably, temperature is 65 DEG C, 80 DEG C or 95 DEG C in the step (2).
Preferably, further comprising the steps of: the soybean protein isolate-chitosan composite particle solution pH value is adjusted
To 3~8.
Preferably, further comprising the steps of: chlorination is added in the soybean protein isolate-chitosan composite particle solution
Sodium makes 0~200mM of its ionic strength.
Preferably, soybean protein isolate-chitosan composite particle partial size that the step (3) obtains be 200~
500nm。
The beneficial effects of the present invention are:
Soybean protein becomes quality plant albumen due to the amino acid of its higher protein content and balance
It represents, it is shown that its huge potential value as meat proteins and lactoprotein substitute.Soybean protein isolate is as soybean
The most important commercialization of albumen represents, and has that nutritive value is high, bio-compatible is good, good emulsibility, foaming characteristic and gel
It the features such as property, can be widely used.With good economic benefit, application value and development prospect.More crucially, due to it
Containing there are many active groups, such as-NH2,-COOH ,-OH and-SH etc., interaction reaction can occur therewith for many substances.Greatly
The isoelectric point of beans protein isolate is 4.5, and as the pH of its aqueous solution < 4.5, soybean protein isolate is positively charged；When its aqueous solution
PH > 4.5 when, soybean protein isolate negatively charged.
Chitosan (Chitosan, CS) also known as chitosan, it is the derivative of chitin deacetylate.Shell
Glycan is unique natural cationic polysaccharide as natural polymer second largest on the earth, with antibiotic property, inoxidizability,
Good biocompatibility, the characteristics such as biodegradable.More importantly, chitosan is due to having-NH on strand2With-OH group
Structure is able to carry out modified, chelating and cross-linking reaction.
When the two is mutually mixed with aqueous solution state, compound polyelectrolyte can be formed, and due to polyelectrolyte
Between can have different interactions, it is possible thereby to formed multiplicity the novel composite particles for having functional characteristic.The present invention
Preparation process it is simple, and there is good mechanical property and water resistance, raw material sources are extensive.
Detailed description of the invention
Fig. 1 is soybean protein isolate-chitosan composite particle grain-size graph under the conditions of different pH value room temperature and 80 DEG C.
Fig. 2 is soybean protein isolate-chitosan composite particle zeta potential diagram under the conditions of different pH value room temperature and 80 DEG C.
Fig. 3 is soybean protein isolate-chitosan composite particle PdI figure under the conditions of different pH value room temperature and 80 DEG C.
Soybean protein isolate-chitosan composite particle grain-size graph under condition of different temperatures when Fig. 4 is pH value 4.55.
Soybean protein isolate-chitosan composite particle zeta potential diagram under condition of different temperatures when Fig. 5 is pH value 4.55.
Soybean protein isolate-chitosan composite particle PdI schemes under condition of different temperatures when Fig. 6 is pH value 4.55.
Fig. 7 is soybean protein isolate-chitosan composite particle Haze curve figure under different pH condition.
Fig. 8 is soybean protein isolate-chitosan composite particle Haze curve figure under the conditions of different ionic strength.
Specific embodiment
For more concise displaying technical solution of the present invention, objects and advantages, combined with specific embodiments below
And attached drawing is described in further detail the present invention.
Embodiment 1
Soybean protein isolate-chitosan composite particle preparation method of the present embodiment, comprising the following steps:
(1) soybean protein isolate for weighing certain mass is dissolved in water, 1% soybean protein isolate solution is configured to, by one
Determine the 100mM acetic acid buffer solution that the chitosan of quality is dissolved in, is configured to 0.2% chitosan solution；By soybean protein isolate
Solution is mixed with chitosan solution by the volume ratio of 5:1, obtains its mixed solution, and adjusts pH value to 4.25.
(2) by the mixed solution of step (1) under the conditions of temperature is 80 DEG C water bath processing 20min.
(3) it is centrifuged after the cooling of above-mentioned mixed solution, takes supernatant molten to get soybean protein isolate-chitosan composite particle
Liquid.
Embodiment 2
Soybean protein isolate-chitosan composite particle preparation method of the present embodiment, except the pH value in step (1) is
Outside 4.55, remaining step and parameter are same as Example 1.
Embodiment 3
Soybean protein isolate-chitosan composite particle preparation method of the present embodiment, except the pH value in step (1) is
Outside 4.9, remaining step and parameter are same as Example 1.
Embodiment 4
Soybean protein isolate-chitosan composite particle preparation method of the present embodiment, except the pH value in step (1) is
4.55, the heating temperature of step (2) is 65 DEG C outer, remaining step and parameter are same as Example 1.
Embodiment 5
Soybean protein isolate-chitosan composite particle preparation method of the present embodiment, except the pH value in step (1) is
4.55, the heating temperature of step (2) is 95 DEG C outer, remaining step and parameter are same as Example 1.
The soybean protein isolate obtained by 1~5 preparation method of above-described embodiment-chitosan composite particle is subjected to performance inspection
It tests, using zeta current potential, partial size and the polydispersion of composite particles in Malvern Nanozetasizer ZS90 measurement supernatant
Property (PDI).
As a result it can see by Fig. 1~3, when system pH is identical, increase temperature, granularity and the zeta electricity of composite particles
Position can increase with it, and significant changes do not occur for PdI.The composite particles that observation Fig. 1 is formed when it is found that being in pH 4.25 are smaller
(d<200nm), and the composite particles formed when pH 4.90 are all bigger (d>200nm), and under the conditions of pH 4.55, with temperature
Raising, the partial size of composite particles also gradually increases to 300nm from 100nm.This explanation is under the conditions of 4.25 pH, heating pair
The influence of SPI-CS system is little, then influences under conditions of 4.90 pH too greatly, and pH 4.55 then can be embodied preferably and be added
Hot bring influences.From Figure 2 it can be seen that whole system is all positively charged, and zeta potential value is greater than+30mV.Zeta current potential can
To be used to evaluate the physical stability of microparticle dispersion system, it is considered that, the absolute value of zeta current potential is higher, interparticle quiet
Electric repulsion effect is bigger, and the physical stability of system is also better, but current potential is not the single factor for influencing dispersion stability,
So to comprehensively consider each index when analysis.From the PdI in Fig. 3 it can also be seen that the PdI value in pH 4.55 exists
In 0.2~0.4 range, show that the size to form particle is more concentrated.The sample that can be determined that preparation by the above results is to receive
Rice grain, method are feasible.
By can see in Fig. 4, the variation of temperature can influence soybean protein isolate-chitosan (SPI-CS) composite particles
Particle size.As the temperature rises, partial size becomes larger, and variation tendency is also gradually increased, and it is compound to illustrate that heating facilitates
The formation of particle.Observation Fig. 5 can see, and the zeta current potential of the nano particle after heating increases, be totally in+30~40mV it
Between, show that system at this time is more stable, but different heating temperature is shown in that variation is not significant.From the PdI in Fig. 6 it can also be seen that
The PdI value of the nano particle of different temperatures heating shows that the size to form particle is more concentrated in 0.2~0.4 range.Pass through
The above results can be determined that heating temperature can be used for regulating and controlling the granule size of nano particle.
Embodiment 6
Soybean protein isolate-chitosan composite particle preparation method of the present embodiment, comprising the following steps:
(1) soybean protein isolate for weighing certain mass is dissolved in water, 1% soybean protein isolate solution is configured to, by one
Determine the 100mM acetic acid buffer solution that the chitosan of quality is dissolved in, is configured to 0.2% chitosan solution；By soybean protein isolate
Solution is mixed with chitosan solution by the volume ratio of 5:1, obtains its mixed solution, and adjusts pH value to 4.55.
(2) by the mixed solution of step (1) under the conditions of temperature is 80 DEG C water bath processing 20min.
(3) it is centrifuged after the cooling of above-mentioned mixed solution, takes supernatant molten to get soybean protein isolate-chitosan composite particle
Liquid.
(4) it is respectively with the supernatant solution pH of 1mol/L HCl solution and 1mol/L NaOH solution difference regulating step (3)
3,4,5,6,7,8, using the turbidity of composite particles in UV-2100 spectrophotometric determination supernatant.
As a result by can see in Fig. 7, heat SPI-CS composite particles have pH responsiveness, are in low turbid in acid condition
The state (solution state) of degree；As pH > 5, the state (aggregation posture) in high concentrtion of heat composite particles processed.This pH responsiveness
As a result illustrate that heat SPI-CS composite particles processed have the embedding of nutriment and provide the ability of gastroenteric environment Targeting delivery.
Embodiment 7
Soybean protein isolate-chitosan composite particle preparation method of the present embodiment, step (1)~(3) with implementation
Example 1 is identical, step (4) are as follows: certain NaCl crystal is added in the supernatant solution in step (3), makes the ionic strength of solution
Respectively become 0,5,10,25,50,100,200mM, using in UV-2100 spectrophotometric determination supernatant composite particles it is turbid
Degree.
As a result by Fig. 8 it can be seen that, heat SPI-CS composite particles processed have certain ionic strength responsiveness, with from
The increase turbidity of sub- intensity increases, and in solution system NaCl concentration < 50mM, it is (molten that heat composite particles are in low turbidity state
Liquid)；As NaCl concentration > 50mM, the state (aggregation posture) in high concentrtion of heat composite particles processed.This ionic strength is rung
Answering property result illustrates that heat SPI-CS composite particles have certain ionic strength resistivity.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of preparation method of soybean protein isolate-chitosan composite particle, which comprises the following steps:
(1) soybean protein isolate solution is mixed with chitosan solution by 2~20:1 volume ratio, obtains its mixed solution, and adjust
PH value is saved to 4~5；
(2) mixed solution of step (1) is heated into 20min under the conditions of temperature is 60 DEG C~100 DEG C；
(3) it is centrifuged after mixed solution cooling, takes supernatant to get soybean protein isolate-chitosan composite particle solution.
2. the preparation method of soybean protein isolate-chitosan composite particle as described in claim 1, which is characterized in that described
Soybean protein isolate solution is prepared using following methods in step (1): being weighed soybean protein isolate and is dissolved in water, is configured to
0.5~2% soybean protein isolate solution.
3. the preparation method of soybean protein isolate-chitosan composite particle as described in claim 1, which is characterized in that described
Chitosan solution is prepared using following methods in step (1): the acetic acid buffer solution that chitosan is dissolved in is configured to 0.1
~1% chitosan solution.
4. the preparation method of soybean protein isolate-chitosan composite particle as claimed in claim 4, which is characterized in that described
The concentration of acetic acid buffer solution is 100mM.
5. the preparation method of soybean protein isolate-chitosan composite particle as described in claim 1, which is characterized in that described
PH value in step (1) is 4.25,4.55 or 4.9.
6. the preparation method of soybean protein isolate-chitosan composite particle as described in claim 1, which is characterized in that described
Heating means in step (2) are heating water bath.
7. the preparation method of soybean protein isolate-chitosan composite particle as described in claim 1, which is characterized in that described
Temperature is 65 DEG C, 80 DEG C or 95 DEG C in step (2).
8. the preparation method of soybean protein isolate-chitosan composite particle as described in claim 1, which is characterized in that also wrap
It includes following steps: the soybean protein isolate-chitosan composite particle solution pH value is adjusted to 3~8.
9. the preparation method of soybean protein isolate-chitosan composite particle as described in claim 1, which is characterized in that also wrap
It includes following steps: sodium chloride is added in the soybean protein isolate-chitosan composite particle solution, make its ionic strength 0
~200mM.
10. the preparation method of soybean protein isolate-chitosan composite particle as described in claim 1, which is characterized in that described
Soybean protein isolate-chitosan composite particle partial size that step (3) obtains is 200~500nm.
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Cited By (5)
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| CN110204908A (en) * | 2019-05-23 | 2019-09-06 | 广州大学 | A kind of preparation method of soybean protein isolate-chitosan Combined Electrostatic gel |
| CN110432377A (en) * | 2019-08-13 | 2019-11-12 | 中国海洋大学 | A kind of soybean protein isolate chitosan nano gel and its preparation method and application |
| CN111955599A (en) * | 2020-08-28 | 2020-11-20 | 广州大学 | Preparation method of acid-soluble soybean protein-chitosan nanoparticles |
| CN111955598A (en) * | 2020-08-28 | 2020-11-20 | 广州大学 | Method for preparing acid-soluble soybean protein by using homogenization method |
| CN113693225A (en) * | 2021-08-06 | 2021-11-26 | 广州大学 | Ovalbumin-ferulic acid-polysaccharide compound emulsion as well as preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110204908A (en) * | 2019-05-23 | 2019-09-06 | 广州大学 | A kind of preparation method of soybean protein isolate-chitosan Combined Electrostatic gel |
| CN110432377A (en) * | 2019-08-13 | 2019-11-12 | 中国海洋大学 | A kind of soybean protein isolate chitosan nano gel and its preparation method and application |
| CN110432377B (en) * | 2019-08-13 | 2020-08-11 | 中国海洋大学 | Soybean protein isolate chitosan nanogel and preparation method and application thereof |
| CN111955599A (en) * | 2020-08-28 | 2020-11-20 | 广州大学 | Preparation method of acid-soluble soybean protein-chitosan nanoparticles |
| CN111955598A (en) * | 2020-08-28 | 2020-11-20 | 广州大学 | Method for preparing acid-soluble soybean protein by using homogenization method |
| CN113693225A (en) * | 2021-08-06 | 2021-11-26 | 广州大学 | Ovalbumin-ferulic acid-polysaccharide compound emulsion as well as preparation method and application thereof |
| CN113693225B (en) * | 2021-08-06 | 2023-12-08 | 广州大学 | Ovalbumin-ferulic acid-polysaccharide compound emulsion and preparation method and application thereof |
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Inventor after: Yuan Yang Inventor after: Li Mengfan Inventor after: He Shan Inventor after: Su Dongxiao Inventor after: Zeng Qingzhu Inventor before: Yuan Chang Inventor before: Li Mengfan Inventor before: He Shan Inventor before: Su Dongxiao Inventor before: Zeng Qingzhu |
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