CN108949892A - A kind of preparation method of microbial flocculant - Google Patents
A kind of preparation method of microbial flocculant Download PDFInfo
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Abstract
The invention discloses a kind of preparation methods of microbial flocculant, belong to microorganisms technical field.The present invention is added sulfuric acid and is polymerize, there is stronger adsorption bridging and net to catch effect, be filled to wadding body hole, so that structure is more closely knit using waterglass as raw material；Using rhodococcus erythropolis, two kinds of the Klebsiella active bacterias with flocculating effect as raw material, the aid nutritions substance such as addition potassium dihydrogen phosphate is precipitated by adsorption bridging, has booster action to sludge reduction；Aspergillus niger, agaric fungus obtain mycelia through the culture of solid support medium, mycelia is wound sphere, on the one hand flocculant activity substance can be loaded, improve flocculant activity, increase lf dose, on the other hand there is biggish specific surface area, increase the ability of mycelium pellet absorption poisonous and harmful substance, improve adsorbance.The present invention solves the problems, such as that the low output for current microbial flocculant, stability are poor.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of preparation method of microbial flocculant.
Background technique
In the control emission of all types of industries waste water, dyeing waste water is China's harmful, intractable Industry Waste main at present
One of water, its main feature is that discharge amount is big, coloration is deep, organic pollutant content is high, change of water quality frequency is big, wherein especially with dyestuff
It pollutes the most serious.With the rapid development of dye industry and the progress of final finishing technology, slurry, New-type adjuvant, dyestuff etc. exist
It is widely used in dyeing.Dyeing waste water complicated component, mainly using aromatic hydrocarbons and heterocyclic compound as parent, and with aobvious
Color base group and polar group.Treatment of dyeing wastewater is mainly for the purpose of decolourizing and reduce COD.Presently used reagent mainly includes taking off
Toner and flocculant are chemical industry polymerization product.The type flocculant is mostly the synthesis such as polyamines, melamine and dicyandiamide
Cationic flocculant, flocculation aid are polyaluminium salts series and polyacrylamide etc..Studies have shown that currently on the market for printing
There is certain toxic side effect in the flocculant and flocculation aid for contaminating wastewater treatment.Especially widely used flocculation aid polyaluminium
Aluminium ion in aluminium has certain neurotoxicity, and the synthon acrylamide of polyacrylamide then has serious " three
Cause " effect.These potential danger all cause huge threat to the balance of the health of the mankind and ecological environment.Microorganism wadding
Solidifying agent is a kind of New Type Water Treatment Chemicals that represent flocculation development direction, it is generated by the Institute of Micro-biology of special metabolic function,
Its function shows as adsorbing suspended particulate, counter ion etc. in liquid system, removal of flocculating.The essence of microbial flocculant
It is large biological molecule, mainly there is protein-based, polysaccharide, glycoprotein, proteoglycan and DNA class etc., is that one kind has nothing
Poison, using it is safe, biodegradable, can large-scale industrial production the features such as New Type Water Treatment Chemicals.And microbial flocculant
Preparation belong to specific function large biological molecule preparation production biotechnology.Currently, many countries to microbial flocculant into
Go research, nearly 80 kinds of the microorganism with flocculability found so far, including bacterium, mould, saccharomycete, actinomyces and algae
Deng, and in flocculating conditions, flocculation mechanism；Flocculant isolates and purifies, property, application；Produce the aspects such as the gene control of flocculant
A series of research is carried out.These are with flocculation activity, the microorganism paid close attention to by people mostly screened from soil, sludge, sewage.
Although microbial flocculant has the characteristics that nontoxic, without secondary pollution and biodegradable, due to its preparation mostly using sugar or
As a raw material for production, high expensive constrains microbial flocculant industrialized production and large-scale application to agricultural and sideline product.To reduce
Production cost needs are set about in terms of two: first is that actively finding cheap raw material, production cost are greatly lowered, second is that needle
To the physicochemical property and purposes of biological flocculant, reasonable industrialization extraction process and application method are selected.
Summary of the invention
The technical problems to be solved by the invention: low output, the problem of stability difference for current microbial flocculant,
A kind of preparation method of microbial flocculant is provided.
In order to solve the above technical problems, the present invention is using technical solution as described below:
A kind of preparation method of microbial flocculant includes the following steps:
(1) water intaking glass 1:5 in mass ratio ~ 8 be added distilled water, 10 ~ 20min of magnetic agitation, add waterglass quality 60 ~
The sulfuric acid that 70% mass fraction is 5%, is stirred 2 ~ 4h in 35 ~ 45 DEG C, stand 18 ~ for 24 hours, obtain standing liquid；
(2) it takes titanium sulfate 1 ~ 3:2 in mass ratio ~ 5:10 ~ 15 that aluminum sulfate, deionized water is added, is stirred, obtains in 35 ~ 45 DEG C
Mixture takes the carboxymethyl cellulose aqueous solution that mass fraction is 2% to be gelatinized in 55 ~ 60 DEG C, obtains dextrin, takes and stands liquid by quality
Mixture, dextrin is added than 10 ~ 20:5 ~ 7:1 ~ 4, heat preservation obtains stirring mixture；
(3) it takes rhodococcus erythropolis 1 ~ 3:2 in mass ratio ~ 5 that Klebsiella mixing is added, obtains Mixed Microbes, take Mixed Microbes by 3 ~ 6%
Inoculum concentration be seeded in seed culture medium, in 25 ~ 33 DEG C, 150r/min cultivate, obtain seed bacterium solution, take Wastewater from Pig Farm by matter
Fermentation medium is added than 1 ~ 3:10 ~ 13 in amount, obtains matrix culture medium, and seed bacterium solution is taken to be seeded to matrix training by 2 ~ 4% inoculum concentration
Fermented and cultured in base is supported, centrifugation takes pellet frozen dry, obtains freeze-drying object, and stirring mixture 2 ~ 5:2 ~ 7 in mass ratio is taken to add
Enter to be freeze-dried object mixing, obtains flocculant matrix object, it is spare；
(4) take aspergillus niger 1 ~ 3:2 in mass ratio ~ 4:10 ~ 13 that agaric fungus, sterile water is added, oscillation obtains oscillation liquid, takes oscillation liquid
It is seeded in PDA liquid medium by 8 ~ 10% inoculum concentration, is cultivated in 25 ~ 28 DEG C, 135r/min, obtain activation culture object, take work
Change culture is seeded in solid support medium by 10 ~ 12% inoculum concentration to be cultivated, and is centrifuged, and is taken pellet frozen dry, must be freeze-dried
Object a；
(5) freeze-drying object a, carrier is added in the flocculant matrix object 1 ~ 3:2 in mass ratio for taking step (2) spare ~ 5:100 ~ 150
Culture medium obtains culture, culture is taken to be freeze-dried to get microbial flocculant in 25 ~ 30 DEG C, 140r/min shaken cultivation.
The stirring condition of stirring mixture in the step (2) are as follows: in 55 ~ 65 DEG C of 10 ~ 12h of heat preservation.
Seed culture medium in the step (3): according to the mass fraction, 8 ~ 13 parts of peptones, 2 ~ 5 portions of beef extracts, 3 ~ 6 parts are taken
Yeast powder, 8 ~ 10 parts of sodium chloride, 1000 parts of water, 121 DEG C of sterilizing 20min.
Fermentation medium in the step (3): according to the mass fraction, take 10 ~ 12 portions of sucrose, 1 ~ 3 part of potassium dihydrogen phosphate, 3 ~
6 parts of dipotassium hydrogen phosphates, 3 ~ 5 parts of ureas, 1 ~ 3 part of yeast powder, 1 ~ 3 part of sodium chloride, 0.1 ~ 0.4 part of magnesium sulfate, 1000 parts of water, 121 DEG C
Sterilize 20min.
The condition of fermented and cultured in the step (3) are as follows: in 25 ~ 33 DEG C, 150r/min fermented and cultured 3 ~ 5 days.
Solid support medium in the step (4): according to the mass fraction, 20 ~ 40 portions of corn flour, 4 ~ 7 parts of wheat brans, 8 ~ 12 parts are taken
Glucose, 0.1 ~ 0.4 part of potassium dihydrogen phosphate, 1 ~ 3 part of ammonium chloride, 0.5 ~ 1.5 part of bitter salt, 1000 parts of water, 121 DEG C go out
Bacterium 20min.
The condition of culture of solid support medium is seeded in the step (4) are as follows: setting magnetic field strength 120mT, in 25 ~ 28
DEG C, 120r/min cultivate 8 ~ 10 days.
The present invention is compared with other methods, and advantageous effects are:
(1) present invention is added sulfuric acid and is polymerize using waterglass as raw material, forms particle greatly and matrix object loosely, tool
There is preferable adsorption bridging ability, titanium sulfate, aluminum sulfate is added, network occurs with the hydroxyl at matrix object chain, ring molecule end
Cooperation is used, and generates that the degree of polymerization with the keys such as Al-O-Si, Ti-O-Si is higher, the longer polymer of strand, is had stronger
The effect of catching of adsorption bridging and net introduces carboxymethyl cellulose bone chain, and enhancing bridging is rolled up the effect of sweeping, is filled to wadding body hole,
So that structure is more closely knit, to be easier to settle, the function of charge neutrality adsorption bridging can occur simultaneously, formed and compare table with larger
The wadding body of area in infall process can net catch, sandwich colloidal particle in water, the performance net effect of catching；
(2) present invention utilizes pig farm using rhodococcus erythropolis, two kinds of the Klebsiella active bacterias with flocculating effect as raw material
Waste water nitrogen abundant and a small amount of carbon, phosphate etc. are used as nutriment, are supplied to two kinds of active bacterias as battalion
The growth elements of substance are supported, the aid nutritions substances such as addition potassium dihydrogen phosphate, dipotassium hydrogen phosphate, urea obtain the higher hair of activity
Ferment object, is added in flocculant, and protein therein, polysaccharose substance form hydrated sheath and electric double layer in water and formed relatively steady
Fixed colloidal solid, then precipitated by adsorption bridging, while it can promote anaerobic granulation, to sludge reduction
Also it is a supporting role；
(3) aspergillus niger of the present invention, agaric fungus obtain mycelia through the culture of solid support medium, and mycelia is wound sphere, are added suitable
Suitable magnetic field strength can promote the growth of microbial cell, improve mycelial yield, increase metabolic activity, promote polysaccharide, protein,
The generation of the beneficial metabolics product such as antibiotic, treated that hyphal cell matter is richer for low-intensity magnetic field, and electron density is higher, cell knot
Structure is complete, and mitochondria is uniformly distributed in into the cell, the spherical-like morphology of thallus, the fast feature of sinking speed is made it have, in mycelia
Between there is also the membrane structure as made of the extracellular polymer material bonding secreted, this special internal structure makes it have more
Big specific surface area and adsorption capacity plays reinforcing, tight to the structure of bulb forms when active material is carried in internal structure
The effect of cause can promote integrality of the mycelium pellet under water flow strong stirring and impact, it is living on the one hand can to load flocculant
Property substance, as bio-carrier, the nutriment of secretion is that flocculation activity bacterium improves nutrition and growth place in carrier, is improved
Flocculant activity increases lf dose, on the other hand has biggish specific surface area, increases mycelium pellet absorption poisonous and harmful substance
Ability, and the inner space of sphere therefore will not be reduced, reduce adsorbance.
Specific embodiment
Rhodococcus erythropolis, Klebsiella, aspergillus niger: it is saved in 2 ~ 4 DEG C of laboratories inclined-plane；Agaric fungus: fresh black fungus is taken
Sterile water is added in 1:8 in mass ratio, and homogenate obtains homogenate.
Wastewater from Pig Farm: through Anaerobic Digestion, every water quality indicator are as follows: pH7.5 ~ 8.0, COD1000 ~ 2000mg/L,
600 ~ 1200mg/L of ammonia nitrogen, 30 ~ 50mg/L of total phosphorus.
Seed culture medium: according to the mass fraction, 8 ~ 13 parts of peptones, 2 ~ 5 portions of beef extracts, 3 ~ 6 parts of yeast powders, 8 ~ 10 parts are taken
Sodium chloride, 1000 parts of water, 121 DEG C of sterilizing 20min.
Fermentation medium: according to the mass fraction, 10 ~ 12 portions of sucrose, 1 ~ 3 part of potassium dihydrogen phosphate, 3 ~ 6 parts of phosphoric acid hydrogen two are taken
Potassium, 3 ~ 5 parts of ureas, 1 ~ 3 part of yeast powder, 1 ~ 3 part of sodium chloride, 0.1 ~ 0.4 part of magnesium sulfate, 1000 parts of water, 121 DEG C of sterilizing 20min.
Solid support medium: according to the mass fraction, take 20 ~ 40 portions of corn flour, 4 ~ 7 parts of wheat brans, 8 ~ 12 parts of glucose, 0.1 ~
0.4 part of potassium dihydrogen phosphate, 1 ~ 3 part of ammonium chloride, 0.5 ~ 1.5 part of bitter salt, 1000 parts of water, 121 DEG C of sterilizing 20min.
A kind of preparation method of microbial flocculant, includes the following steps:
(1) water intaking glass 1:5 in mass ratio ~ 8 be added distilled water, 10 ~ 20min of magnetic agitation, add waterglass quality 60 ~
The sulfuric acid that 70% mass fraction is 5%, is stirred 2 ~ 4h in 35 ~ 45 DEG C, stand 18 ~ for 24 hours, obtain standing liquid；
(2) take titanium sulfate 1 ~ 3:2 in mass ratio ~ 5:10 ~ 15 that aluminum sulfate, deionized water is added, it is stirred 30 in 35 ~ 45 DEG C ~
40min obtains mixture, takes the carboxymethyl cellulose aqueous solution that mass fraction is 2% in 55 ~ 60 DEG C of 1 ~ 2h of gelatinization, obtains dextrin, take
Standing liquid 10 ~ 20:5 in mass ratio ~ 7:1 ~ 4 addition mixture, dextrin must be stirred in 55 ~ 65 DEG C of 10 ~ 12h of heat preservation
Object；
(3) it takes rhodococcus erythropolis 1 ~ 3:2 in mass ratio ~ 5 that Klebsiella mixing is added, obtains Mixed Microbes, take Mixed Microbes by 3 ~ 6%
Inoculum concentration be seeded in seed culture medium, cultivated 2 ~ 3 days in 25 ~ 33 DEG C, 150r/min, obtain seed bacterium solution, take pig farm useless
Fermentation medium is added in water 1 ~ 3:10 in mass ratio ~ 13, obtains matrix culture medium, seed bacterium solution is taken to be seeded to by 2 ~ 4% inoculum concentration
In matrix culture medium, in 25 ~ 33 DEG C, 150r/min fermented and cultured 3 ~ 5 days, centrifugation took pellet frozen dry, must be freeze-dried
Object takes stirring mixture 2 ~ 5:2 in mass ratio ~ 7 that freeze-drying object mixing is added, obtains flocculant matrix object, spare；
(4) it takes aspergillus niger 1 ~ 3:2 in mass ratio ~ 4:10 ~ 13 that agaric fungus, sterile water is added, vibrates 5 ~ 10min, obtain oscillation liquid, take
Oscillation liquid is seeded in PDA liquid medium by 8 ~ 10% inoculum concentration, is cultivated 5 ~ 7 days, must be activated in 25 ~ 28 DEG C, 135r/min
Culture takes activation culture object to be seeded in solid support medium by 10 ~ 12% inoculum concentration, and magnetic field strength 120mT is arranged, in 25 ~
28 DEG C, 120r/min culture 8 ~ 10 days, centrifugation takes pellet frozen dry, must be freeze-dried object a；
(5) freeze-drying object a, carrier is added in the flocculant matrix object 1 ~ 3:2 in mass ratio for taking step (2) spare ~ 5:100 ~ 150
Culture medium obtains culture in 25 ~ 30 DEG C, 140r/min shaken cultivation 2 ~ 4 days, takes culture to be freeze-dried to get microorganism wadding
Solidifying agent.
Rhodococcus erythropolis, Klebsiella, aspergillus niger: it is saved in 2 DEG C of laboratories inclined-plane；Agaric fungus: take fresh black fungus by
Sterile water is added in mass ratio 1:8, and homogenate obtains homogenate.
Wastewater from Pig Farm: through Anaerobic Digestion, every water quality indicator are as follows: pH7.5, COD1000mg/L, ammonia nitrogen 600mg/
L, total phosphorus 30mg/L.
Seed culture medium: according to the mass fraction, take 8 parts of peptones, 2 portions of beef extracts, 3 parts of yeast powders, 8 parts of sodium chloride,
1000 parts of water, 121 DEG C of sterilizing 20min.
Fermentation medium: according to the mass fraction, take 10 portions of sucrose, 1 part of potassium dihydrogen phosphate, 3 parts of dipotassium hydrogen phosphates, 3 parts of ureas,
1 part of yeast powder, 1 part of sodium chloride, 0.1 part of magnesium sulfate, 1000 parts of water, 121 DEG C of sterilizing 20min.
Solid support medium: according to the mass fraction, 20 portions of corn flour, 4 parts of wheat brans, 8 parts of glucose, 0.1 part of biphosphate are taken
Potassium, 1 part of ammonium chloride, 0.5 part of bitter salt, 1000 parts of water, 121 DEG C of sterilizing 20min.
A kind of preparation method of microbial flocculant, includes the following steps:
(1) distilled water is added in water intaking glass 1:5 in mass ratio, and magnetic agitation 10min adds the quality of waterglass quality 60%
The sulfuric acid that score is 5% is stirred 2h in 35 DEG C, stands 18h, obtains standing liquid；
(2) it takes titanium sulfate 1:2:10 in mass ratio that aluminum sulfate, deionized water is added, is stirred 30min in 35 DEG C, must mix
Object takes the carboxymethyl cellulose aqueous solution that mass fraction is 2% in 55 DEG C of gelatinization 1h, obtains dextrin, takes and stands liquid in mass ratio 10:
5:1 is added mixture, dextrin and obtains stirring mixture in 55 DEG C of heat preservation 10h；
(3) take rhodococcus erythropolis 1:2 in mass ratio be added Klebsiella mixing, obtain Mixed Microbes, take Mixed Microbes by 3% inoculation
Amount is seeded in seed culture medium, is cultivated 2 days in 25 DEG C, 150r/min, is obtained seed bacterium solution, take Wastewater from Pig Farm in mass ratio 1:
10 are added fermentation medium, obtain matrix culture medium, take seed bacterium solution to be seeded in matrix culture medium by 2% inoculum concentration, in 25
DEG C, 150r/min fermented and cultured 3 days, centrifugation takes pellet frozen dry, obtains freeze-drying object, takes stirring mixture in mass ratio
Freeze-drying object mixing is added in 2:2, obtains flocculant matrix object, spare；
(4) it takes aspergillus niger 1:2:10 in mass ratio that agaric fungus, sterile water is added, vibrates 5min, obtain oscillation liquid, take oscillation liquid by 8%
Inoculum concentration be seeded in PDA liquid medium, in 25 DEG C, 135r/min cultivate 5 days, obtain activation culture object, take activation culture
Object is seeded in solid support medium by 10% inoculum concentration, and magnetic field strength 120mT is arranged, and is cultivated 8 days in 25 DEG C, 120r/min, from
The heart takes pellet frozen dry, must be freeze-dried object a；
(5) freeze-drying object a, solid support medium is added in the flocculant matrix object 1:2:100 in mass ratio for taking step (2) spare,
In 25 DEG C, 140r/min shaken cultivation 2 days, culture is obtained, culture is taken to be freeze-dried to get microbial flocculant.
Rhodococcus erythropolis, Klebsiella, aspergillus niger: it is saved in 3 DEG C of laboratories inclined-plane；Agaric fungus: take fresh black fungus by
Sterile water is added in mass ratio 1:8, and homogenate obtains homogenate.
Wastewater from Pig Farm: through Anaerobic Digestion, every water quality indicator are as follows: pH7.8, COD1500mg/L, ammonia nitrogen 900mg/
L, total phosphorus 40mg/L.
Seed culture medium: according to the mass fraction, take 11 parts of peptones, 4 portions of beef extracts, 5 parts of yeast powders, 9 parts of sodium chloride,
1000 parts of water, 121 DEG C of sterilizing 20min.
Fermentation medium: according to the mass fraction, take 11 portions of sucrose, 2 parts of potassium dihydrogen phosphates, 5 parts of dipotassium hydrogen phosphates, 4 parts of ureas,
2 parts of yeast powders, 2 parts of sodium chloride, 0.3 part of magnesium sulfate, 1000 parts of water, 121 DEG C of sterilizing 20min.
Solid support medium: according to the mass fraction, 30 portions of corn flour, 6 parts of wheat brans, 10 parts of glucose, 0.3 part of biphosphate are taken
Potassium, 2 parts of ammonium chlorides, 1.0 parts of bitter salts, 1000 parts of water, 121 DEG C of sterilizing 20min.
A kind of preparation method of microbial flocculant, includes the following steps:
(1) distilled water is added in water intaking glass 1:7 in mass ratio, and magnetic agitation 15min adds the quality of waterglass quality 65%
The sulfuric acid that score is 5% is stirred 3h in 40 DEG C, stands 21h, obtains standing liquid；
(2) it takes titanium sulfate 2:4:13 in mass ratio that aluminum sulfate, deionized water is added, is stirred 35min in 40 DEG C, must mix
Object takes the carboxymethyl cellulose aqueous solution that mass fraction is 2% in 58 DEG C of gelatinization 1.5h, obtains dextrin, takes and stands liquid in mass ratio
15:6:3 is added mixture, dextrin and obtains stirring mixture in 60 DEG C of heat preservation 11h；
(3) take rhodococcus erythropolis 2:4 in mass ratio be added Klebsiella mixing, obtain Mixed Microbes, take Mixed Microbes by 5% inoculation
Amount is seeded in seed culture medium, is cultivated 2.5 days in 29 DEG C, 150r/min, is obtained seed bacterium solution, take Wastewater from Pig Farm in mass ratio
Fermentation medium is added in 2:12, obtains matrix culture medium, takes seed bacterium solution to be seeded in matrix culture medium by 3% inoculum concentration, in 29
DEG C, 150r/min fermented and cultured 4 days, centrifugation takes pellet frozen dry, obtains freeze-drying object, takes stirring mixture in mass ratio
Freeze-drying object mixing is added in 4:5, obtains flocculant matrix object, spare；
(4) it takes aspergillus niger 2:3:12 in mass ratio that agaric fungus, sterile water is added, vibrates 8min, obtain oscillation liquid, take oscillation liquid by 9%
Inoculum concentration be seeded in PDA liquid medium, in 27 DEG C, 135r/min cultivate 6 days, obtain activation culture object, take activation culture
Object is seeded in solid support medium by 11% inoculum concentration, and magnetic field strength 120mT is arranged, and is cultivated 9 days in 27 DEG C, 120r/min, from
The heart takes pellet frozen dry, must be freeze-dried object a；
(5) freeze-drying object a, solid support medium is added in the flocculant matrix object 2:4:130 in mass ratio for taking step (2) spare,
In 28 DEG C, 140r/min shaken cultivation 3 days, culture is obtained, culture is taken to be freeze-dried to get microbial flocculant.
Rhodococcus erythropolis, Klebsiella, aspergillus niger: it is saved in 4 DEG C of laboratories inclined-plane；Agaric fungus: take fresh black fungus by
Sterile water is added in mass ratio 1:8, and homogenate obtains homogenate.
Wastewater from Pig Farm: through Anaerobic Digestion, every water quality indicator are as follows: pH8.0, COD2000mg/L, ammonia nitrogen
1200mg/L, total phosphorus 50mg/L.
Seed culture medium: according to the mass fraction, take 13 parts of peptones, 5 portions of beef extracts, 6 parts of yeast powders, 10 parts of sodium chloride,
1000 parts of water, 121 DEG C of sterilizing 20min.
Fermentation medium: according to the mass fraction, take 12 portions of sucrose, 3 parts of potassium dihydrogen phosphates, 6 parts of dipotassium hydrogen phosphates, 5 parts of ureas,
3 parts of yeast powders, 3 parts of sodium chloride, 0.4 part of magnesium sulfate, 1000 parts of water, 121 DEG C of sterilizing 20min.
Solid support medium: according to the mass fraction, 40 portions of corn flour, 7 parts of wheat brans, 12 parts of glucose, 0.4 part of biphosphate are taken
Potassium, 3 parts of ammonium chlorides, 1.5 parts of bitter salts, 1000 parts of water, 121 DEG C of sterilizing 20min.
A kind of preparation method of microbial flocculant, includes the following steps:
(1) distilled water is added in water intaking glass 1:8 in mass ratio, and magnetic agitation 20min adds the quality of waterglass quality 70%
The sulfuric acid that score is 5% is stirred 4h in 45 DEG C, stands for 24 hours, obtains standing liquid；
(2) it takes titanium sulfate 3:5:15 in mass ratio that aluminum sulfate, deionized water is added, is stirred 40min in 45 DEG C, must mix
Object takes the carboxymethyl cellulose aqueous solution that mass fraction is 2% in 60 DEG C of gelatinization 2h, obtains dextrin, takes and stands liquid in mass ratio 20:
7:4 is added mixture, dextrin and obtains stirring mixture in 65 DEG C of heat preservation 12h；
(3) take rhodococcus erythropolis 3:5 in mass ratio be added Klebsiella mixing, obtain Mixed Microbes, take Mixed Microbes by 6% inoculation
Amount is seeded in seed culture medium, is cultivated 3 days in 33 DEG C, 150r/min, is obtained seed bacterium solution, take Wastewater from Pig Farm in mass ratio 3:
13 are added fermentation medium, obtain matrix culture medium, take seed bacterium solution to be seeded in matrix culture medium by 4% inoculum concentration, in 33
DEG C, 150r/min fermented and cultured 5 days, centrifugation takes pellet frozen dry, obtains freeze-drying object, takes stirring mixture in mass ratio
Freeze-drying object mixing is added in 5:7, obtains flocculant matrix object, spare；
(4) take aspergillus niger 3:4:13 in mass ratio be added agaric fungus, sterile water, vibrate 10min, obtain oscillation liquid, take oscillation liquid by
10% inoculum concentration is seeded in PDA liquid medium, is cultivated 7 days in 28 DEG C, 135r/min, is obtained activation culture object, activation is taken to train
It supports object to be seeded in solid support medium by 12% inoculum concentration, magnetic field strength 120mT is set, in 28 DEG C, 120r/min culture 10
It, centrifugation takes pellet frozen dry, must be freeze-dried object a；
(5) freeze-drying object a, solid support medium is added in the flocculant matrix object 3:5:150 in mass ratio for taking step (2) spare,
In 30 DEG C, 140r/min shaken cultivation 4 days, culture is obtained, culture is taken to be freeze-dried to get microbial flocculant.
Comparative example: the microbial flocculant of certain company production.
By microbial flocculant obtained by embodiment 1,2,3 and comparative example in the ratio of 300ml/L put into pH value be 7.5,
Temperature is to observe its lf dose in 25 DEG C of waste water；It takes 500ml to be placed in 5 DEG C of refrigerators again to save, its flocculation effect was detected every 1 month
Fruit, continuous detection 3 months.Its testing result record such as table 1.
Table 1:
In conclusion as shown in Table 1, existing product increases lf dose to microbial flocculant prepared by the present invention more in the market,
And comprehensive flocculating effect, 90% or more, stability gets a promotion, and has biggish development prospect.
Claims (7)
1. a kind of preparation method of microbial flocculant, which is characterized in that the preparation method includes the following steps:
(1) water intaking glass 1:5 in mass ratio ~ 8 be added distilled water, 10 ~ 20min of magnetic agitation, add waterglass quality 60 ~
The sulfuric acid that 70% mass fraction is 5%, is stirred 2 ~ 4h in 35 ~ 45 DEG C, stand 18 ~ for 24 hours, obtain standing liquid；
(2) it takes titanium sulfate 1 ~ 3:2 in mass ratio ~ 5:10 ~ 15 that aluminum sulfate, deionized water is added, is stirred, obtains in 35 ~ 45 DEG C
Mixture takes the carboxymethyl cellulose aqueous solution that mass fraction is 2% to be gelatinized in 55 ~ 60 DEG C, obtains dextrin, takes and stands liquid by quality
Mixture, dextrin is added than 10 ~ 20:5 ~ 7:1 ~ 4, heat preservation obtains stirring mixture；
(3) it takes rhodococcus erythropolis 1 ~ 3:2 in mass ratio ~ 5 that Klebsiella mixing is added, obtains Mixed Microbes, take Mixed Microbes by 3 ~ 6%
Inoculum concentration be seeded in seed culture medium, in 25 ~ 33 DEG C, 150r/min cultivate, obtain seed bacterium solution, take Wastewater from Pig Farm by matter
Fermentation medium is added than 1 ~ 3:10 ~ 13 in amount, obtains matrix culture medium, and seed bacterium solution is taken to be seeded to matrix training by 2 ~ 4% inoculum concentration
Fermented and cultured in base is supported, centrifugation takes pellet frozen dry, obtains freeze-drying object, and stirring mixture 2 ~ 5:2 ~ 7 in mass ratio is taken to add
Enter to be freeze-dried object mixing, obtains flocculant matrix object, it is spare；
(4) take aspergillus niger 1 ~ 3:2 in mass ratio ~ 4:10 ~ 13 that agaric fungus, sterile water is added, oscillation obtains oscillation liquid, takes oscillation liquid
It is seeded in PDA liquid medium by 8 ~ 10% inoculum concentration, is cultivated in 25 ~ 28 DEG C, 135r/min, obtain activation culture object, take work
Change culture is seeded in solid support medium by 10 ~ 12% inoculum concentration to be cultivated, and is centrifuged, and is taken pellet frozen dry, must be freeze-dried
Object a；
(5) freeze-drying object a, carrier is added in the flocculant matrix object 1 ~ 3:2 in mass ratio for taking step (2) spare ~ 5:100 ~ 150
Culture medium obtains culture, culture is taken to be freeze-dried to get microbial flocculant in 25 ~ 30 DEG C, 140r/min shaken cultivation.
2. the preparation method of microbial flocculant according to claim 1, which is characterized in that stirring in the step (2)
The stirring condition of mixture are as follows: in 55 ~ 65 DEG C of 10 ~ 12h of heat preservation.
3. the preparation method of microbial flocculant according to claim 1, which is characterized in that seed in the step (3)
Culture medium: according to the mass fraction, 8 ~ 13 parts of peptones, 2 ~ 5 portions of beef extracts, 3 ~ 6 parts of yeast powders, 8 ~ 10 parts of sodium chloride, 1000 are taken
Part water, 121 DEG C of sterilizing 20min.
4. the preparation method of microbial flocculant according to claim 1, which is characterized in that fermentation in the step (3)
Culture medium: according to the mass fraction, 10 ~ 12 portions of sucrose, 1 ~ 3 part of potassium dihydrogen phosphate, 3 ~ 6 parts of dipotassium hydrogen phosphates, 3 ~ 5 parts of ureas, 1 ~ 3 are taken
Part yeast powder, 1 ~ 3 part of sodium chloride, 0.1 ~ 0.4 part of magnesium sulfate, 1000 parts of water, 121 DEG C of sterilizing 20min.
5. the preparation method of microbial flocculant according to claim 1, which is characterized in that fermentation in the step (3)
The condition of culture are as follows: in 25 ~ 33 DEG C, 150r/min fermented and cultured 3 ~ 5 days.
6. the preparation method of microbial flocculant according to claim 1, which is characterized in that carrier in the step (4)
Culture medium: according to the mass fraction, 20 ~ 40 portions of corn flour, 4 ~ 7 parts of wheat brans, 8 ~ 12 parts of glucose, 0.1 ~ 0.4 part of biphosphate are taken
Potassium, 1 ~ 3 part of ammonium chloride, 0.5 ~ 1.5 part of bitter salt, 1000 parts of water, 121 DEG C of sterilizing 20min.
7. the preparation method of microbial flocculant according to claim 1, which is characterized in that inoculation in the step (4)
To the condition of culture of solid support medium are as follows: setting magnetic field strength 120mT is cultivated 8 ~ 10 days in 25 ~ 28 DEG C, 120r/min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112280804A (en) * | 2020-09-30 | 2021-01-29 | 神美科技有限公司 | Preparation method and application of microbial flocculant |
| CN113860519A (en) * | 2021-11-09 | 2021-12-31 | 重庆沐兰环保科技有限公司 | Efficient microbial composite flocculant and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112280804A (en) * | 2020-09-30 | 2021-01-29 | 神美科技有限公司 | Preparation method and application of microbial flocculant |
| CN113860519A (en) * | 2021-11-09 | 2021-12-31 | 重庆沐兰环保科技有限公司 | Efficient microbial composite flocculant and preparation method thereof |
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