CN108949639B - Acinetobacter baumannii for degrading aureomycin and application thereof - Google Patents

Acinetobacter baumannii for degrading aureomycin and application thereof Download PDF

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CN108949639B
CN108949639B CN201810948822.0A CN201810948822A CN108949639B CN 108949639 B CN108949639 B CN 108949639B CN 201810948822 A CN201810948822 A CN 201810948822A CN 108949639 B CN108949639 B CN 108949639B
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aureomycin
acinetobacter baumannii
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李永涛
龚贝妮
宋桐慧
史艳财
彭桂香
张玉龙
王进进
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South China Agricultural University
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Abstract

The invention discloses acinetobacter baumannii for degrading aureomycin and application thereof. The strain is named as Acinetobacter baumannii (Acinetobacter baumannii) F3, and the preservation number is GDMCC NO:60375, the strain is preserved in Guangdong province microbial strain preservation center of No. 59 building 5 of Tokyo No. 100 college of Cedrury of Middy, Guangzhou, 5.23.2018. The acinetobacter baumannii can degrade aureomycin in a co-metabolism mode, and can achieve 95.57% degradation rate in 20 hours and 99.04% degradation rate in 35 hours for 10mg/L low-concentration aureomycin; meanwhile, the strain also has potassium-dissolving performance, and is beneficial to increasing soil fertility. Therefore, the acinetobacter baumannii can be applied to removing residual aureomycin in liquid and soil, and has the functions of increasing soil potassium nutrient elements and enhancing soil fertility when being applied to the soil.

Description

Acinetobacter baumannii for degrading aureomycin and application thereof
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to acinetobacter baumannii for degrading aureomycin and application thereof.
Background
Antibiotics are widely used in the livestock industry at present, and tetracycline antibiotics are one of the most commonly used antibiotic types. The tetracycline antibiotics entering into livestock body are mostly discharged out of body in the form of excrement and urine, and the livestock excrement containing tetracycline is directly used as fertilizer in farmland, and the undegraded drugs in excrement and urine are original orThe biologically active metabolites will then enter the soil system or further into the groundwater system. According to investigation, the residue of tetracycline antibiotics in the manure is basically 0.03-563.8 mg-kg-1Within the range, the residual quantity of the aureomycin can reach 563.8 mg/kg-1(Chen et al, 2012; Hu et al, 2010; Coxin et al, 2011; Liuxin Cheng et al, 2008; Zhang Hui Min et al, 2008). According to the investigation of tetracycline antibiotics in soil of partial regions, the residual concentration of the tetracycline in the soil is the highest.
The method for degrading aureomycin residue in the environment by using a microbial method has wide prospect. At present, a few reports are made on the screening of aureomycin degrading strains. However, the currently reported aureomycin degrading strains are all of the mould types. The strain related to the invention is the bacterium acinetobacter baumannii. Reports are not found at home and abroad.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide the acinetobacter baumannii for degrading aureomycin.
The invention also aims to provide application of the acinetobacter baumannii for degrading aureomycin.
The purpose of the invention is realized by the following technical scheme: acinetobacter baumannii (Acinetobacter baumannii) F3 for degrading aureomycin, wherein the accession number is GDMCC NO:60375, the strain is preserved in Guangdong province microbial strain preservation center of No. 59 building 5 of Tokyo No. 100 college of Cedrury of Middy, Guangzhou, 5.23.2018.
A method for culturing the acinetobacter baumannii for degrading aureomycin comprises the following specific steps: inoculating acinetobacter baumannii capable of degrading aureomycin into a culture medium, and culturing at the temperature of 30-44 ℃;
the culture medium is LB culture medium.
The culture conditions are preferably: culturing at 30 deg.C for 20 h.
The acinetobacter baumannii for degrading aureomycin is applied to potassium decomposition.
The acinetobacter baumannii for degrading aureomycin is applied to the improvement of the content of available potassium elements in soil, and the acinetobacter baumannii has a potassium-dissolving performance, so that the content of nutrient elements in the soil can be improved, and the soil fertility is improved.
The acinetobacter baumannii for degrading aureomycin is applied to degrading aureomycin.
The application of the acinetobacter baumannii for degrading aureomycin in aureomycin degradation is to add the acinetobacter baumannii for degrading aureomycin into soil or water.
The soil is the soil containing aureomycin, and the water body is the water body containing aureomycin; the acinetobacter baumannii has the performance of degrading aureomycin and can be used for degrading aureomycin with low concentration in soil and water, so that the residue of aureomycin in the environment is reduced, and the effect of treating environmental pollution is achieved.
A biological degrading bacteria agent of aureomycin contains the Acinetobacter baumannii for degrading aureomycin.
The 16S rDNA sequence of the aureomycin degrading strain F3 of the invention is as follows (SEQ ID NO. 1):
agaatttccggggcgaccgtggtaccgccctctttgcagttaggctagctacttctggtgcaacaaactcccatggtgtgacgggcggtgtgtacaaggcccgggaacgtattcaccgcggcattctgatccgcgattactagcgattccgacttcatggagtcgagttgcagactccaatccggactacgatcggctttttgagattagcatcacatcgctgtgtagcaaccctttgtaccgaccattgtagcacgtgtgtagccctggccgtaagggccatgatgacttgacgtcgtccccgccttcctccagtttgtcactggcagtatccttaaagttcccatccgaaatgctggcaagtaaggaaaagggttgcgctcgttgcgggacttaacccaacatctcacgacacgagctgacgacagccatgcagcacctgtatctagattcccgaaggcaccaatccatctctggaaagtttctagtatgtcaaggccaggtaaggttcttcgcgttgcatcgaattaaaccacatgctccaccgcttgtgcgggcccccgtcaattcatttgagttttagtcttgcgaccgtactccccaggcggtctacttatcgcgttagctgcgccactaaagcctcaaaggccccaacggctagtagacatcgtttacggcatggactaccagggtatctaatcctgtttgctccccatgctttcgtacctcagcgtcagtattaggccagatggctgccttcgccatcggtattcctccagatctctacgcatttcaccgctacacctggaattctaccatcctctcccatactctagctcaccagtatcgaatgcaattcccaagttaagctcggggatttcacatccgacttaataagccgcctacgcacgctttacgcccagtaaatccgattaacgctcgcaccctctgtattaccgcggctgctggcacagagttagccggtgcttattctgcgagtaacgtccactatctctaggtattaactaaagtagcctcctcctcgcttaaagtgctttacaaccataaggccttcttcacacacgcggcatggctggatcagggttccccccattgtccaatattccccactgctgcctcccgtaggagtctgggccgtgtctcagtcccagtgtggcggatcatcctctcagacccgctacagatcgtcgccttggtaggcctttaccccaccaactagctaatccgacttaggctcatctattagcgcaaggtccgaagatcccctgctttctcccgtaggacgtatgcggtattagcatccctttcgagatgttgtcccccactaataggcagattcctaagcattactcacccgtccgccgctaggtccggtagcaagctaccttcccccgctcgactgcaga。
the above sequence consists of 1427 bases (bp).
When the acinetobacter baumannii strain F3 grows on an LB flat plate, the colony of the acinetobacter baumannii strain is milky white, round and small in volume, and the surface of the acinetobacter baumannii strain is convex outwards, smooth and glossy. The growth can be carried out at 30 ℃ and 44 ℃. F3 was found to be a short rod by electron microscopy. Can grow well in inorganic salt liquid culture medium containing peptone with aureomycin concentration of 10 mg/L.
Compared with the prior art, the invention has the following advantages and effects: the strain F3 can degrade aureomycin in a co-metabolism mode, the strain F3 is inoculated in an inorganic salt liquid culture medium containing peptone and the aureomycin concentration of 10mg/L, shaking culture is carried out at 30 ℃ and 150r/min for 20h, the aureomycin degradation rate reaches 95.57%, the aureomycin degradation rate reaches 99.04% 35h after adding the strain, and the aureomycin degradation rate of F3 is obviously improved compared with that of a control without adding the strain. The bacterial strain F3 has the performance of degrading aureomycin, can be applied to the degradation of low-concentration aureomycin in soil and water, and the degradation effect of the bacterial strain F3 on the low-concentration aureomycin can reduce the residue of the aureomycin in the environment. The strain F3 also has potassium-dissolving performance and is beneficial to increasing soil fertility. Therefore, the strain F3 can be applied to removing residual aureomycin in liquid and soil, and has the functions of increasing soil potassium nutrient elements and enhancing soil fertility when being applied to soil.
Drawings
FIG. 1 is a colony morphology map of strain F3.
FIG. 2 is a phylogenetic tree of strain F3.
FIG. 3 is a gram stain of strain F3.
FIG. 4 is a graph showing the growth of the strain F3 on a potassium feldspar-containing medium (hydrolysis ring appearing around the colony).
FIG. 5 is a graph showing the degradation rate of aureomycin by the strain F3 in 10-PMSM medium and the growth of bacteria (CK represents no bacteria addition control).
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. The following examples are given by way of illustration and are given in the art of routine experimentation and procedures unless otherwise indicated.
Example 1 screening, isolation and purification and identification of Strain F3
Screening method of aureomycin degradation strain F3
1. Material preparation
The sludge source of the bacteria is obtained from sludge of first-stage, second-stage and third-stage oxidation ponds of Wen-shi Luo Chen pig farms in New county, Yunhuo City, Guangdong province, and the sludge is taken back and packaged by self-sealing bags and stored at the temperature of-20 ℃ for later use.
LB culture medium: 10.0g of tryptone, 5.0g of yeast extract powder, 10.0g of NaCl, 1000mL of distilled water, pH 7.0-7.2 and 18g of agar powder added in a solid culture medium. Sterilizing at 121 deg.C for 15 min.
Inorganic salt medium (MSM): 5mL of phosphoric acid buffer solution (KH)2PO48.5g·L-1、K2HPO4·H2O21.75g·L-1、Na2HPO4·12H2O 33.4g·L-1、NH4Cl 5.0g·L-1)，3.0mL 22.5g·L-1MgSO (2) of4Solution (MgSO)4·7H2O 46.125g·L-1)，1.0mL 0.25g·L-1FeCl of3Solution (FeCl)3·6H2O 0.42g·L-1)，1.0mL 36.4g·L-1In (C) is2Solution (CaCl)2·2H2O 48.22g·L-1) 1.0mL of a solution of trace elements (MnSO)4·H2O 39.9mg·L-1；ZnSO4·H2O 42.8mg·L-1；(NH4)6Mo7O24·4H2O 34.7mg·L-1) After the mixture is uniform, the volume is fixed to 1L by pure water, and the mixture is stored at 4 ℃ for later use.
10-PMSM medium: 10g tryptone was added to 1000mL of MSM medium solution to prepare a solution containing 10 g.L-1An inorganic salt culture medium of tryptone.
0.2mol·L-1Disodium hydrogen phosphate solution of (1): 71.6g of disodium hydrogen phosphate (containing 12 crystal waters) is weighed, dissolved and then the volume is determined to be 1L.
0.1mol·L-1Citric acid solution: 21.01g of citric acid is weighed and dissolved to a constant volume of 1L.
Mclvaine buffer: 1000mL of 0.1 mol. L-1Citric acid and 625mL 0.2 mol.L-1Disodium hydrogen phosphate was mixed and the pH was adjusted to 4.0 ± 0.05 with NaOH or HCl.
0.1mol·L-1Na2EDTA-Mclvaine buffer: 60.0g of disodium edetate is weighed into 1625ml of LMcllvaine buffer solution, dissolved by ultrasonic and shaken up.
2. Laboratory apparatus and device
Vertical pressure steam sterilization pan (BL-50A, Shanghai Silique industries, Ltd.), portable pH meter (PHB-4, Shanghai precision science, Ltd.), Centrifuge (Centrifuge 5810R), electric heating oven (DGG-9070A, Shanghai Senxin experiment apparatus, Ltd.), digital display constant temperature water bath pan (HH series, Changzhou national apparatus manufacturing Co., Ltd.), refrigerator (RCD-205AG7, Hai Xin electric appliance), biochemical incubator (PYX-208S-A, Keli apparatus), clean bench (SW-CJ-1F, Sujing Antai air technology, Ltd.), voro mutex mixer (XW-80A, Shanghai Jing industries, Ltd.), MyCycler PCR (BIO-RAD, USA), electrophoresis apparatus (DYY-6C, Bei six instrument works), NaDrno nucleic acid protein quantitative detector (German rmmo), Germany, Gel imaging system (BIO-RAD, USA), floor type constant temperature oscillator (HZQ-211C).
3. Enrichment screening, separation and purification of aureomycin degradation strain
(1) Isolation and purification of the strains
Preparing sludge collected from first, second and third oxidation ponds of Wen-shi Luo Chen pig farm in east town of emerging county of Yunhuo City of Guangdong provinceAfter forming soil suspension, add 5, 10, 20 mg.L-1The concentration of aureomycin is gradually increased in an MSM inorganic salt culture medium for domestication and culture. After acclimatization and screening, 1mL of culture solution is added into 9mL of sterile water for dilution until the dilution is 10-7Thus, 0.1mL of the bacterial suspension was applied to a nutrient agar medium and incubated at a constant temperature for 20 hours, and then the morphological characteristics of the colonies were visually observed.
(2) Strain screening
Inoculating the single colony obtained by separation and purification in the step (1) into an LB culture medium for activation culture. Adding into aureomycin at a concentration of 10 mg.L at a ratio of 2% (v/v) after activation-1) Culturing in 10-PMSM culture medium at 150 r.min-1And (3) culturing in a shaking table (constant temperature of 30 ℃) in a dark place, measuring the concentration of the aureomycin in the solution by using a High Performance Liquid Chromatography (HPLC) method after 24 hours, and calculating the degradation rate of the strain to the aureomycin. Obtaining the strain F3 which can degrade aureomycin with high efficiency.
The aureomycin determination method comprises the following steps: firstly, 0.1 mol.L of a sample is put-1Na of (2)2Mixing EDTA-Mclvaine buffer solution with equal volume, oscillating for 5min, performing ultrasonic extraction for 30min at 10000 r.min-1Centrifuging for 10min, and collecting supernatant. Then passing through HLB column, and using 5.0mL methanol and 5.0mL 0.1 mol.L-1Na of (2)2The EDTA-Mclvaine buffer solution activates the HLB column. After passing through the column, the column was rinsed with 6mL of ultrapure water, dried at room temperature for 15min, and the sample was eluted with 1/5 volumes of methanol. The column for the solution to be tested was E clipse XDBC18 (4.6X 250mm, 5 μm), the A phase was 0.1% (V/V) formic acid aqueous solution, the B phase was V (methanol): V (acetonitrile): 2:3, V (A): V (B): 2:1, flow rate 1 mL/min-1The column temperature is 35 ℃; the sample injection amount is 10 mu L; under the condition that the detection wavelength is 375nm, the peaks of the aureomycin and other substances can be completely separated by an ultraviolet detector, and the retention time of the aureomycin is 4.9-5.0 min.
Secondly, observing morphological characteristics of bacterial colonies and identifying gram staining
The strain F3 grows faster on LB medium and can grow at 30 ℃ and 44 ℃. The bacterial colony is milky white, round, small in volume, and convex, smooth and glossy in surface (figure 1); the results of gram staining indicated that F3 was a gram negative bacterium (fig. 3).
Thirdly, molecular biological identification of the strain F3
DNA extraction
The total DNA of the thalli is extracted by adopting a bacterial genome DNA extraction kit (centrifugal column type, Tiangen Biochemical technology company).
2.16S rDNA amplification
And (3) amplifying by using the extracted total DNA of the bacteria as a template and adopting a bacterial 16S rDNA universal primer, wherein the forward primer is 27 f: 5'-AGAGTTTGATCCTGGCTCAG-3', reverse primer 1492 r: 5'-GGTTACCTTGTTACGACTT-3' (Stackelbrandt et al, 1991) amplified 16S rRNA gene sequences.
The total PCR reaction volume is 50. mu.L; 2 mu L of each of the upper primer and the lower primer; 2 mu L of template DNA; 2 × Taq PCR Master Mix25 μ L; sterilized 21. mu.L of high purity water.
The PCR reaction program is: pre-denaturation at 94 ℃ for 5min, denaturation at 94 ℃ for 30s, extension at 72 ℃ for 90s, 30 cycles. Extension was supplemented at 72 ℃ for 10 min. The PCR product was detected by 1.0% agarose gel electrophoresis and DL2000Marker was selected. The obvious bands appear in the range between 1000bp and 2000bp bands of the Marker observed under a gel imaging system.
3.16 determination of the S rDNA sequence
The PCR amplified product was sent to Huada Biotech company (Guangzhou) for sequencing. The 16S rDNA gene sequence of the obtained strain is as follows: agaatttccggggcgaccgtggtaccgccctctttgcagttaggctagctacttctggtgcaacaaactcccatggtgtgacgggcggtgtgtacaaggcccgggaacgtattcaccgcggcattctgatccgcgattactagcgattccgacttcatggagtcgagttgcagactccaatccggactacgatcggctttttgagattagcatcacatcgctgtgtagcaaccctttgtaccgaccattgtagcacgtgtgtagccctggccgtaagggccatgatgacttgacgtcgtccccgccttcctccagtttgtcactggcagtatccttaaagttcccatccgaaatgctggcaagtaaggaaaagggttgcgctcgttgcgggacttaacccaacatctcacgacacgagctgacgacagccatgcagcacctgtatctagattcccgaaggcaccaatccatctctggaaagtttctagtatgtcaaggccaggtaaggttcttcgcgttgcatcgaattaaaccacatgctccaccgcttgtgcgggcccccgtcaattcatttgagttttagtcttgcgaccgtactccccaggcggtctacttatcgcgttagctgcgccactaaagcctcaaaggccccaacggctagtagacatcgtttacggcatggactaccagggtatctaatcctgtttgctccccatgctttcgtacctcagcgtcagtattaggccagatggctgccttcgccatcggtattcctccagatctctacgcatttcaccgctacacctggaattctaccatcctctcccatactctagctcaccagtatcgaatgcaattcccaagttaagctcggggatttcacatccgacttaataagccgcctacgcacgctttacgcccagtaaatccgattaacgctcgcaccctctgtattaccgcggctgctggcacagagttagccggtgcttattctgcgagtaacgtccactatctctaggtattaactaaagtagcctcctcctcgcttaaagtgctttacaaccataaggccttcttcacacacgcggcatggctggatcagggttccccccattgtccaatattccccactgctgcctcccgtaggagtctgggccgtgtctcagtcccagtgtggcggatcatcctctcagacccgctacagatcgtcgccttggtaggcctttaccccaccaactagctaatccgacttaggctcatctattagcgcaaggtccgaagatcccctgctttctcccgtaggacgtatgcggtattagcatccctttcgagatgttgtcccccactaataggcagattcctaagcattactcacccgtccgccgctaggtccggtagcaagctaccttcccccgctcgactgcaga are provided.
The above sequence consists of 1427 bases (bp).
And carrying out BLAST comparison on the obtained sequencing result of the 16S rDNA gene sequence in a webpage of the National Center for Biotechnology Information (NCBI), carrying out homology comparison analysis on the sequence of the 16S rDNA gene of a related model strain in an LPSN database (http:// www.bacterio.net/index. html), downloading the sequence of the model strain with higher homology, carrying out BLAST comparison and homology analysis on the sequence of an amplification product on a website of the National Center for Biotechnology Information (NCBI), and adopting Mega 6.0 software to construct a phylogenetic tree by a Neighbour-Joining method. Comparison of the 16S rDNA sequence revealed that strain F3 has 99% homology with Acinetobacter baumannii (FIG. 2).
Fourthly, identifying the strain F3 as a new functional strain
According to the colony morphological characteristics and molecular biological identification results of the strain F3, the strain F3 is identified as Acinetobacter baumannii (Acinetobacter baumannii) and named as Acinetobacter baumannii F3(Acinetobacter baumannii F3). The strain has been preserved in Guangdong province microorganism culture Collection (GDMCC), with the accession number of GDMCC No. 60375, and the preservation date of 2018, 5 months and 23 days. The address of the preservation unit is No. 59 building No. 5 building of No. 100 college of Jifura Zhonglu, Guangzhou city.
At present, no document reports that acinetobacter baumannii has the function of degrading aureomycin at home and abroad. Therefore, the acinetobacter baumannii is a new strain with the function of degrading aureomycin.
Example 2 determination of phosphorus and Potassium solubilizing abilities of Strain F3
1. Qualitative test of phosphorus dissolving function of the strain: inoculating the strain into an LB culture medium by a scribing method, culturing for 24 hours under the condition of a 37 ℃ culture box, then selecting a single colony spot to be inoculated on a PKO solid culture medium, setting 3 repeated tests, placing in the 37 ℃ culture box for 5-7 days, observing whether a transparent ring formed by hydrolysis of dissolved phosphorus exists around a colony in the culture medium, observing the size of the transparent ring, measuring the diameters of a hydrolysis ring and the colony ring by using a ruler, and taking a picture. And judging whether the bacterial strain has phosphorus dissolving effect and relative size of phosphorus dissolving capacity according to the diameter ratio of the hydrolysis ring to the bacterial colony ring. Wherein the content of the first and second substances,
PKO culture medium formula: glucose 10 g.L-1，(NH4)2SO40.5g·L-1，NaCl 0.2g·L-1，MgSO4·7H2O 0.1g·L-1，KCl 0.3g·L-1，MnSO4·4H2O 0.03g·L-1，FeSO40.003g·L-1，Ca3(PO4)25.0g·L-10.5 g.L of yeast powder-120.0 g.L of agar powder-1，pH 6.8～7.0。
2. The qualitative test of the potassium-dissolving capacity of the strain comprises the following steps: inoculating the strain on an LB culture medium by using a plate-scribing method, placing the strain in an incubator at 37 ℃ for culturing for 24 hours, then selecting a single colony spot to be connected to a potassium-dissolved solid culture medium, setting 3 repeated tests, placing the strain in the incubator at 37 ℃ for 5-7 days, observing a transparent ring formed by potassium-dissolved hydrolysis around a colony in the culture medium, observing the size of the transparent ring, measuring the diameters of the hydrolysis ring and the colony ring by using a ruler, and taking a picture. And judging whether the bacterial strain has the potassium-decomposing effect and the relative size of the potassium-decomposing capacity according to the diameter ratio of the hydrolysis ring to the bacterial colony ring. Wherein the content of the first and second substances,
the potassium dissolving culture medium formula comprises: potassium feldspar 2.5 g.L-1，Na2HPO40.2g·L-1，MgSO4·7H2O 0.02g·L-1，NaCl 0.2g·L-1，CaCO35.0g·L-1，CaSO4·2H2O 0.1g·L-1Glucose 10 g.L-120.0 g.L of agar powder-1，pH6.8～7.0。
The results show that the strain F3 has no phosphorus dissolving performance but has potassium dissolving capacity (figure 4), and the ratio of the potassium dissolving hydrolysis ring to the colony ring is 8.16.
EXAMPLE 3 growth of Strain F3 in mineral salts Medium containing aureomycin and peptone and its degradation of aureomycin
Activating the frozen strain F3 on LB plate for 20h, selecting single strain, culturing in LB culture medium at 150 r.min-1, 30 deg.C for 20h, and inoculating to aureomycin with content of 10 mg.L at a ratio of 2% (v/v)-1Containing 10 g.L-1Culturing tryptone in MSM culture medium (10-PMSM culture medium) for 35 hr, and sampling every 5 times to determine its OD600(ii) a The residual amount of aureomycin was determined by HPLC after solution extraction using the strain F3 as a control (aureomycin assay as in example 1).
The result shows that the aureomycin without adding the bacteria is hydrolyzed, and the degradation effect of the aureomycin is obviously accelerated and strengthened after adding the bacteria F3. 95.57% had been reached at 20h, while the control had only hydrolyzed 30.47%, and a 99.04% degradation rate was reached 35h after addition of the bacteria, while only 57.52% was degraded by hydrolysis. From OD600The change in values indicated that strain F3 grew rapidly, the maximum density of growth had been reached for 5h, after which the bacterial density remained stable, but biodegradation continued (FIG. 5).
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Sequence listing
<110> southern China university of agriculture
<120> Acinetobacter baumannii for degrading aureomycin and application thereof
<160>1
<170>SIPOSequenceListing 1.0
<210>1
<211>1427
<212>DNA
<213> Acinetobacter baumannii (Acinetobacter baumannii)
<220>
<223> Acinetobacter baumannii (Acinetobacter baumannii) F3
<400>1
agaatttccg gggcgaccgt ggtaccgccc tctttgcagt taggctagct acttctggtg 60
caacaaactc ccatggtgtg acgggcggtg tgtacaaggc ccgggaacgt attcaccgcg 120
gcattctgat ccgcgattac tagcgattcc gacttcatgg agtcgagttg cagactccaa 180
tccggactac gatcggcttt ttgagattag catcacatcg ctgtgtagca accctttgta 240
ccgaccattg tagcacgtgt gtagccctgg ccgtaagggc catgatgact tgacgtcgtc 300
cccgccttcc tccagtttgt cactggcagt atccttaaag ttcccatccg aaatgctggc 360
aagtaaggaa aagggttgcg ctcgttgcgg gacttaaccc aacatctcac gacacgagct 420
gacgacagcc atgcagcacc tgtatctaga ttcccgaagg caccaatcca tctctggaaa 480
gtttctagta tgtcaaggcc aggtaaggtt cttcgcgttg catcgaatta aaccacatgc 540
tccaccgctt gtgcgggccc ccgtcaattc atttgagttt tagtcttgcg accgtactcc 600
ccaggcggtc tacttatcgc gttagctgcg ccactaaagc ctcaaaggcc ccaacggcta 660
gtagacatcg tttacggcat ggactaccag ggtatctaat cctgtttgct ccccatgctt 720
tcgtacctca gcgtcagtat taggccagat ggctgccttc gccatcggta ttcctccaga 780
tctctacgca tttcaccgct acacctggaa ttctaccatc ctctcccata ctctagctca 840
ccagtatcga atgcaattcc caagttaagc tcggggattt cacatccgac ttaataagcc 900
gcctacgcac gctttacgcc cagtaaatcc gattaacgct cgcaccctct gtattaccgc 960
ggctgctggc acagagttag ccggtgctta ttctgcgagt aacgtccact atctctaggt 1020
attaactaaa gtagcctcct cctcgcttaa agtgctttac aaccataagg ccttcttcac 1080
acacgcggca tggctggatc agggttcccc ccattgtcca atattcccca ctgctgcctc 1140
ccgtaggagt ctgggccgtg tctcagtccc agtgtggcgg atcatcctct cagacccgct 1200
acagatcgtc gccttggtag gcctttaccc caccaactag ctaatccgac ttaggctcat 1260
ctattagcgc aaggtccgaa gatcccctgc tttctcccgt aggacgtatg cggtattagc 1320
atccctttcg agatgttgtc ccccactaat aggcagattc ctaagcatta ctcacccgtc 1380
cgccgctagg tccggtagca agctaccttc ccccgctcga ctgcaga 1427

Claims (7)

1. An acinetobacter baumannii strain for degrading aureomycin, which is characterized in that: the name is Acinetobacter baumannii (Acinetobacter baumannii) F3, the preservation number is GDMCC NO:60375, the strain is preserved in Guangdong province microbial strain preservation center of No. 59 building 5 of Tokyo No. 100 college of Cedrury of Middy, Guangzhou, 5.23.2018.
2. The method for culturing the acinetobacter baumannii for degrading aureomycin according to claim 1, which is characterized by comprising the following specific steps: inoculating acinetobacter baumannii capable of degrading aureomycin into a culture medium, and culturing at the temperature of 30-44 ℃; the culture medium is LB culture medium.
3. Use of acinetobacter baumannii degrading aureomycin according to claim 1 for increasing the content of available potassium element in soil.
4. The use of acinetobacter baumannii degrading aureomycin according to claim 1 for degrading aureomycin.
5. The use of acinetobacter baumannii for degrading aureomycin according to claim 4, wherein: adding acinetobacter baumannii for degrading aureomycin into soil or water.
6. The use of acinetobacter baumannii for degrading aureomycin according to claim 5, wherein: the soil is the soil containing aureomycin; the water body is a water body containing aureomycin.
7. A aureomycin biodegradation microbial inoculum is characterized in that: acinetobacter baumannii containing the aureomycin degrading agent according to claim 1.
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