CN108948670A - A kind of preparation method and application of insect anatomy ware - Google Patents
A kind of preparation method and application of insect anatomy ware Download PDFInfo
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- CN108948670A CN108948670A CN201810855062.9A CN201810855062A CN108948670A CN 108948670 A CN108948670 A CN 108948670A CN 201810855062 A CN201810855062 A CN 201810855062A CN 108948670 A CN108948670 A CN 108948670A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L63/00—Compositions of epoxy resins; Compositions of derivatives of epoxy resins
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/02—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group
- C08L2205/025—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group containing two or more polymers of the same hierarchy C08L, and differing only in parameters such as density, comonomer content, molecular weight, structure
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of preparation method and applications of insect anatomy ware, it is to mix the A glue of epoxide resin AB mixed type crystal flexible glue and B glue according to the ratio of weight 3: 1, it is poured into clean culture dish after mixing evenly, then culture dish is put into vacuum pump and removes bubble removing, horizontal stationary is placed, and insect anatomy ware is obtained after colloid solidification.The features such as insect anatomy ware prepared using the method for the present invention is had transparency high, does not shrink, do not warp, and flexibility is good, easy to clean.Dissecting ware has smooth glue surface and certain flexibility; cleaning and disinfection when convenient for anatomic tissue; fine dissection forceps tips are protected not to be damaged; and dissection ware transparency is high; translucency is good when using under Stereo microscope, is particularly suitable for carrying out the dissection of insect tissue using fine dissection tweezers.When dissected is carried out using this dissection ware, physiological saline first is added dropwise on dissection ware, then carry out insect tissue dissection, the insect tissue of physiological activity can be obtained.
Description
Technical field
The present invention relates to a kind of preparation method and applications of insect anatomy ware, belong to entomotomy field.
Background technique
Insect is the animal population of most species in the animal kingdom, and insect known to the mankind has more than 100 ten thousand kinds, to agricultural production
Significant impact is caused with human health.By taking agricultural insect planthopper important on rice as an example, planthopper can pass through piercing and sucking rice
Juice causes symptoms such as " lice burnings ", causes the rice underproduction.In addition, also to propagate stripe disease, rice secret note short for planthopper
A variety of Virus Diseases of Rice, the transmitted virus diseases such as contracting virosis, southern rice black-streaked dwarf disease and rice tingia dwarf wilt cause
Harm be even higher than insect itself harm.
Insect specific organization is dissected and is studied the cause evil mechanism to parsing insect to play a significant role.It is currently known
Plant virus there are about 1100 kinds, propagated wherein about 80% plant virus relies on insect vector, Some Insects can will be sick
Poison is transmitted to offspring.Sustained proliferation type plant virus enters esophagus by insect's food-taking, it is thin to infect epithelium across middle intestines microvillus
Born of the same parents simultaneously establish First aggression point, and the muscle cell that basilar memebrane reaches intestinal tract surface is passed through after proliferation, is diffused into then along muscle fibre
Entire alimentary canal is discharged into hemolymph, invades salivary gland, enters plant phloem with insect's food-taking.By anatomic tissue and exempt from
Epidemic disease fluorescent marker etc. the study found that the fungal component Sulcia of leafhopper can directly interact with fractilinea oryzae, and
It carries virus and enters leafhopper egg mother cell [1], the virion of tomato yellow leaf curl virus and rice stripe virus by epithelial tissue
Son can infect egg mother cell, to carry out vertical transmission [2,3] with vitellogenin (Vg) interaction of mediator insect.In addition,
By dissection to small brown rice planthopper fat-body, haemocyte, ovary etc. and the study found that small brown rice planthopper female adult haemocyte can produce it is abundant
Vg simultaneously finally mediates RSV to propagate [4] by ovum.
Currently, the tissue of insect is obtained generally by using chemical fixer that polypide is fixed, it may shadow
Ring its physiological activity.Its anatomic tissue of insect biggish for figure is relatively easy, and insect lesser for figure, and dissection is then
It needs to be dissected under Stereo microscope using fine dissection tweezers.As planthopper is long by most of only 3 at polypide fructus forsythiae
~5mm, tissue as head, middle intestines, fat-body in equal volume if it is smaller.Since dissection tweezers front end is very tapering, directly hard
Culture dish on dissected and will lead to the bending of tweezers leading portion quickly, and then be not available.The present invention is dissected for insect tissue
Present in this problem, provide a kind of preparation method of insect anatomy ware, utilize this dissection ware carry out insect tissue solution
It cuts open, solves the problems, such as to cause damage to tweezers when anatomic tissue of the figure compared with small insect, upper physiological saline is dripped on dissection ware
Insect tissue dissection is carried out afterwards, and analog insect vivo environment guarantees the physiological activity of insect tissue, is used for follow-up study.
Bibliography:
1, Jia D, Mao Q, Chen Y, Liu Y, Chen Q, Wu W, Zhang X, Chen H, Li Y.Wei
T.Insect symbiotic bacteria harbours viral pathogens for transovarial
Transmission.Nature Microbiology, 2017,2:17025.
2, Wei J, He YZ, Guo Q, Guo T, Liu YQ, Zhou XP, Liu SS, Wang XW.Vector
development and vitellogenin determine the transovarial transmission of
Begomoviruses.Proc Natl Acad Sci U S A, 2017,114 (26): 6746-6751.
3, Huo Y, Liu W, Zhang F, Chen X, Li L, Liu Q, Zhou Y, Wei T, Fang R, Wang
X.Transovarial transmission of a plant virus is mediated by vitellogenin of
Its insect vector.PLoS Pathogens.2014,10 (3): e1003949.
4, HuoY, Yu Y, Chen L, Li Q, Zhang M, Song Z, Chen X, Fang R, Zhang L.Insect
tissue-specific vitellogenin facilitates transmission of plant virus.PLoS
Pathog.2018,14 (2): e1006909.
Summary of the invention
The present invention is directed to the status currently without specific insect anatomy ware, provides a kind of preparation side of insect anatomy ware
Method mainly prepares insect anatomy ware using epoxide resin AB mixed type crystal glue-dropping (flexible glue).This dissection ware is in anatomic tissue
It cleans and sterilizes very convenient, and fine dissection tweezers can be protected not to be damaged in when dissected.
The invention is realized by the following technical scheme:
A kind of preparation method of insect anatomy ware, comprising the following steps:
1, epoxy resin A glue and B glue are weighed according to 3: 1 ratio of weight, wherein B glue is coagulator；
2, A glue and B glue are mixed in clean small beaker, is poured into clean culture dish after mixing evenly；
3, it is put into vacuum pump and removes bubble removing；
4,6~8 hours of horizontal rest obtain insect anatomy ware to colloid solidification.
The insect anatomy ware prepared using the method for the present invention is had transparency high, does not shrink, do not warp, flexibility is good, easily
The features such as cleaning.The cleaning and disinfection when the smooth glue surface of ware is convenient for anatomic tissue are dissected, dissection ware has certain flexibility,
Fine dissection forceps tips can be protected not to be damaged, be particularly suitable for carrying out the solution of insect tissue using fine dissection tweezers
It cuts open.The insect physiological saline that 10~20 μ l are added dropwise on ware is dissected herein, forms it into an elliptical bead.The elder brother that will be dissected
Worm is placed in of short duration pre-cooling in ice face, so that it is carried out of short duration suspend mode and is placed in the drop of physiological saline, raw in analog insect bodies
Environment is managed, dissection obtains the insect tissue for having physiological activity.
The above description is only an overview of the technical scheme of the present invention, shows method of the invention in order to become apparent from, and can
It is implemented in accordance with the contents of the specification, specific embodiment is shown in detail by following embodiment.
Specific embodiment
Embodiment 1 carries out the dissection of small brown rice planthopper midgut tissue with dissection ware
According to 3: 1 ratio of weight, 6g epoxy resin A glue and 2g epoxy resin B glue are weighed with beaker.B glue is poured into containing A
In the beaker of glue, is stirred evenly at once with glass rod along the same direction, pour into glass culture dish or plastic culture dish, AB glue is poured into
Height be about the 2/3 of culture dish height, culture dish is placed in vacuum pump and is vacuumized, removes the bubble in solution, level puts
It sets, static 8 hours, after AB glue solidification, insect anatomy ware is made.
When production dissection ware, culture dish size can select the culture dish of different-diameter as needed.Present case is to insect ash
Plant hopper is dissected, since small brown rice planthopper figure is smaller, the long only 4~5mm of body, therefore select diameter for the culture dish of 60cm.
Small brown rice planthopper is placed in ice face and is placed into of short duration suspend mode, 10~20 μ l physiology salts are then added dropwise on insect anatomy ware
Water forms it into circular drop, then the small brown rice planthopper in suspend mode is put into, and destroys small brown rice planthopper head and chest using tapering dissection tweezers
Portion junction, separates head with thorax abdomen, and as breach, another tweezers clamps tail portion, by entire intestinal tissue from tail
Portion pulls out, and midgut tissue is chosen in normal saline buffer solution.
The above description is only an embodiment of the present invention, is not intended to restrict the invention, and comes for those skilled in the art
It says, the invention may be variously modified and varied.It is all within principle of the invention, made any modification, changes equivalent replacement
Into etc., it should all be included in the protection scope of the present invention.
Claims (3)
1. a kind of preparation method of insect anatomy ware, feature the following steps are included:
1) by weight 3: 1 ratio weighs the A glue and B glue of epoxide resin AB mixed type crystal flexible glue respectively；
2) it stirs evenly, sol solution is poured into clean culture dish after A glue and the mixing of B glue；
3) culture dish is put into vacuum pump and is vacuumized, remove the bubble in solution；
4) culture dish after vacuumizing is horizontally arranged, insect anatomy ware is obtained after colloid solidification.
2. the preparation method of insect anatomy ware according to claim 1, it is characterised in that: step 4 culture dish is horizontal
Standing time is 6-8 hours.
3. method prepares the application of resulting dissection ware according to claim 1, it is characterised in that: physiology salt is added dropwise on dissection ware
The insect of suspend mode of short duration after freezing is placed in physiological saline the dissection for carrying out insect tissue using fine dissection tweezers by water.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810855062.9A CN108948670A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method and application of insect anatomy ware |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810855062.9A CN108948670A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method and application of insect anatomy ware |
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| CN201810855062.9A Pending CN108948670A (en) | 2018-07-27 | 2018-07-27 | A kind of preparation method and application of insect anatomy ware |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003092892A1 (en) * | 2002-04-30 | 2003-11-13 | Greiner Bio - One Gmbh | Device for protein crystallisation |
| CN206064448U (en) * | 2016-08-25 | 2017-04-05 | 广东省农业科学院植物保护研究所 | A kind of low temperature gel dissecting pan |
| CN106810821A (en) * | 2016-12-21 | 2017-06-09 | 东华大学 | The high grade of transparency, nanofiber-filled amount high cooperate with the preparation method of enhanced composite |
| CN107474323A (en) * | 2017-09-29 | 2017-12-15 | 肇庆医学高等专科学校 | A kind of dissecting pan and preparation method thereof |
-
2018
- 2018-07-27 CN CN201810855062.9A patent/CN108948670A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003092892A1 (en) * | 2002-04-30 | 2003-11-13 | Greiner Bio - One Gmbh | Device for protein crystallisation |
| CN206064448U (en) * | 2016-08-25 | 2017-04-05 | 广东省农业科学院植物保护研究所 | A kind of low temperature gel dissecting pan |
| CN106810821A (en) * | 2016-12-21 | 2017-06-09 | 东华大学 | The high grade of transparency, nanofiber-filled amount high cooperate with the preparation method of enhanced composite |
| CN107474323A (en) * | 2017-09-29 | 2017-12-15 | 肇庆医学高等专科学校 | A kind of dissecting pan and preparation method thereof |
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Application publication date: 20181207 |