CN108941607A - A method of nano Au particle is prepared by template of Daptomycin - Google Patents
A method of nano Au particle is prepared by template of Daptomycin Download PDFInfo
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- CN108941607A CN108941607A CN201810948948.8A CN201810948948A CN108941607A CN 108941607 A CN108941607 A CN 108941607A CN 201810948948 A CN201810948948 A CN 201810948948A CN 108941607 A CN108941607 A CN 108941607A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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Abstract
The invention discloses a kind of methods for preparing nano Au particle as template using Daptomycin, belong to nano metal material technical field.The present invention comprises the following steps: using Daptomycin aqueous solution as template, chlorauric acid solution is presoma, two kinds of solution is mixed with out mixed solution in proportion, incubation with heat makes the combination of the active site and presoma of template, addition reducing agent is reacted, and the solution containing nano Au particle is made.Preparation condition of the present invention is mild, easy to operate, reaction process is easily controllable；Use polypeptide (Daptomycin) for template, it is low in cost, pollution-free；The partial size of the gold nanoparticle of preparation is 2-15nm, specific surface area with higher；The morphological rules of obtained gold nanoparticle, uniform particle sizes have high stability；Prepared gold nanoparticle has higher absorption peak at 500-600nm, has good photo-thermal effect.
Description
Technical field
The invention belongs to nano metal material technical field, in particular to a kind of preparation method of nano material.
Background technique
Nano material shows since itself has quantum size effect, skin effect and macro quanta tunnel effect etc.
Many distinctive physical properties, chemical property out.When gold nanoparticle and incident wavelength interact, will occur surface etc. from
Sub-resonance causes internal crystal framework to resonate, and obtains big energy, to generate photo-thermal effect.The photo-thermal effect of nanoparticle can
It is widely used in treatment of cancer, drug delivery, chemical vapor deposition and nano-photoetching etc..
The preparation method of traditional gold nanoparticle be Turkevich and Frens propose use citric acid in aqueous solution
The method that salt restores gold chloride, i.e. make reducing agent also makees stabilizer to sodium citrate.Not only preparation condition is more harsh for this method, needs
Heating stirring is condensed back；And the gold nanoparticle of preparation easily occurs to gather in acid condition or high ionic strength environment
Collection.Holding stablizes and is the premise item that gold nanoparticle is applied to field of biomedicine with biocompatibility in physiological environment
Part.One of the effective ways for obtaining the preferable gold nanoparticle of stability are exactly that some dressing agents are added in the preparation to its surface
It is modified, however there are still some disadvantages for these method of modifying.In addition to this, the common preparation method of gold nanoparticle is also
Chemical gas-phase method, laser method, Chemical liquid deposition, sol-gel method etc..Appealing preparation method, there are technical requirements height, if
It is standby expensive, complex process, the larger disadvantage of environmental pollution.
Summary of the invention
The present invention provides a kind of side that nano Au particle is prepared using Daptomycin as template to solve above-mentioned technical problem
Method, its object is to keep experimental provision simple, operating condition is mild；Another object be to make the size of nano material, pattern and
Structure can be controlled accurately.
The specific technical proposal of the invention is: a kind of method for preparing nano Au particle as template using Daptomycin, special
Sign is to comprise the following steps: using Daptomycin aqueous solution as template, chlorauric acid solution is presoma, in proportion by two kinds of solution
It is mixed with out mixed solution, incubation with heat makes the combination of the active site and presoma of template, and addition reducing agent is reacted,
The solution containing nano Au particle is made.
A further technical solution lies in comprise the following steps: Daptomycin aqueous solution is adjusted pH=1~13；And chlorine
Auric acid solution mixes in proportion, puts it into metal bath, is incubated for 0~48 hour at 50~1000 revs/min, 0 DEG C~80 DEG C；
Reducing agent is added dropwise in solution after being incubated for, until solution colour no longer changes, nano Au particle solution is made.
A further technical solution lies in the Daptomycin aqueous solution is using Daptomycin and deionized water according to quality
It is configured than 1:333~10000；Daptomycin solution and chlorauric acid solution are the ratio mixing of 0.05~20:1 in molar ratio.
A further technical solution lies in, to Daptomycin aqueous solution hydrochloric acid and or sodium hydrate regulator solution pH
=1~13.
A further technical solution lies in the reducing agent is dimethyamine borane or sodium borohydride.
It is 50~1000 revs/min a further technical solution lies in the condition that, the metal bath is incubated for process, 0 DEG C~80
It is incubated for 0~48 hour at DEG C.
The invention has the following advantages over the prior art:
1, preparation condition is mild, easy to operate, reaction process is easily controllable.
2, use polypeptide (Daptomycin) for template, it is low in cost, pollution-free.
3, the partial size of the gold nanoparticle prepared is 2-15nm, specific surface area with higher.
4, the morphological rules of obtained gold nanoparticle, uniform particle sizes have high stability.
5, prepared gold nanoparticle has higher absorption peak at 500-600nm, has good photo-thermal effect.
Detailed description of the invention
Fig. 1 is Daptomycin-gold nanoparticle TEM figure that the embodiment of the present invention 1 obtains.
Fig. 2 is Daptomycin-gold nanoparticle transmission electron microscope particle diameter distribution histogram that the embodiment of the present invention 1 obtains.
Fig. 3 is that temperature is at any time under 808nm illumination for the Daptomycin-solution of gold nanoparticles that obtains of the embodiment of the present invention 1
Between variation diagram.
Fig. 4 is Daptomycin-gold nanoparticle TEM figure that the embodiment of the present invention 2 obtains.
Fig. 5 is Daptomycin-gold nanoparticle transmission electron microscope particle diameter distribution histogram that the embodiment of the present invention 2 obtains.
Fig. 6 is Daptomycin-gold nanoparticle TEM figure that the embodiment of the present invention 3 obtains.
Fig. 7 is Daptomycin-gold nanoparticle transmission electron microscope particle diameter distribution histogram that the embodiment of the present invention 3 obtains.
Fig. 8 is that temperature is at any time under 808nm illumination for the Daptomycin-solution of gold nanoparticles that obtains of the embodiment of the present invention 4
Between variation diagram.
Fig. 9 is Daptomycin-gold nanoparticle TEM figure that the embodiment of the present invention 4 obtains.
Figure 10 is Daptomycin-gold nanoparticle transmission electron microscope particle diameter distribution histogram that the embodiment of the present invention 4 obtains.
Figure 11 is Daptomycin-gold nanoparticle ultraviolet-visible extinction spectrum figure that the embodiment of the present invention 4 obtains.
Specific embodiment
Embodiment 1
By Daptomycin and deionized water to be mixedly configured into Daptomycin according to the ratio that mass ratio is 1:333 water-soluble
Liquid draws 400 μ L daptomycin solutions in 2ml centrifuge tube, according to daptomycin solution and chlorauric acid solution 1:2 in molar ratio
Ratio mixing controlled total volume 1.8ml, be put into metal bath with the hydrochloric acid conditioning solution PH=2 of 1M, control revolving speed is 600
Rev/min, temperature is incubated for 10min under the conditions of being 5 DEG C；Dimethyamine borane is added at last 25 DEG C, until color no longer becomes
It turns to only, obtains gold nanoparticle.
The nano-particle solution that the above process is obtained is placed in the cuvette of 1 × 1cm.In 808nm near infrared light in 2w
Lower irradiation 10min, every minor tick 10s record a temperature.It maps to temperature and time:
It is as shown in Figure 1 transmission electron microscope to Daptomycin-gold nanoparticle morphology characterization；
If Fig. 2 is Daptomycin-gold nanoparticle particle diameter distribution histogram, the average diameter of nano Au particle is
4.38nm.Morphological rules, in spherical；
As shown in figure 3, showing that Daptomycin-gold nanoparticle has good photo-thermal effect.
Embodiment 2
Daptomycin is mixed with deionized water according to mass ratio for 1:1000, is configured to daptomycin solution, absorption reaches
The ratio of 600 μ L of Tobramycin solution and chlorauric acid solution 2:1 in molar ratio mix, and are put into gold after adjusting pH=3 with the hydrochloric acid of 1M
Belong in bath, control total volume 1.8ml, control revolving speed is 600 revs/min, and temperature is incubated for 10min under the conditions of being 0 DEG C；It is eventually adding two
Methyl amine borine obtains gold nanoparticle until color is no longer changed.
The nano-particle solution that the above process is obtained is placed in 1 × 1 cuvette.In 808nm near infrared light at 2w
10min is irradiated, every minor tick 10s records a temperature.It maps to temperature and time:
Transmission electron microscope is illustrated in figure 4 to Daptomycin-gold nanoparticle morphology characterization；
If Fig. 5 is Daptomycin-gold nanoparticle particle diameter distribution histogram, the average diameter of nano Au particle is
7.68nm.Morphological rules, in spherical.
Embodiment 3
Daptomycin is mixed with deionized water according to mass ratio for 1:1800, daptomycin solution is configured to, 1ml is taken to reach
The ratio of Tobramycin solution and chlorauric acid solution 4:1 in molar ratio mix, and are put into metal bath, control after adjusting PH=4 with hydrochloric acid
Revolving speed processed is 500 revs/min, and temperature is incubated for 10min under the conditions of being 60 DEG C；The last solution toward after being incubated for is added dimethyl and pacifies boron
Alkane no longer changes to its color.
The nano-particle solution that the above process is obtained is placed in the cuvette of 1 × 1cm.In 808nm near infrared light in 2w
Lower irradiation 10min, every minor tick 10s record a temperature.It maps to temperature and time:
Transmission electron microscope is illustrated in figure 6 to Daptomycin-gold nanoparticle morphology characterization；
If Fig. 7 is Daptomycin-gold nanoparticle particle diameter distribution histogram, the average diameter of nanometer platinum particles is
4.96nm.Morphological rules, in spherical；
As shown in figure 8, showing that Daptomycin-gold nanoparticle has good photo-thermal effect.
Embodiment 4
Daptomycin is mixed with deionized water according to mass ratio for 1:1000, Daptomycin aqueous solution is configured to, takes 800
The ratio of μ L daptomycin solution and chlorauric acid solution 4:1 in molar ratio mix, and are put into metal bath after adjusting pH=3 with hydrochloric acid
In, control revolving speed is 600 revs/min, and temperature is incubated for 10h under the conditions of being 60 DEG C；Dimethyl peace is added in the last solution toward after being incubated for
Borine no longer changes to its color.
The nano-particle solution that the above process is obtained is placed in 1 × 1 cuvette.In 808nm near infrared light at 2w
10min is irradiated, every minor tick 10s records a temperature.It maps to temperature and time:
Transmission electron microscope is illustrated in figure 9 to Daptomycin-gold nanoparticle morphology characterization；
If Figure 10 is Daptomycin-gold nanoparticle particle diameter distribution histogram, the average diameter of nano Au particle is
8.47nm.Morphological rules, in spherical；
As shown in figure 11, show that Daptomycin-gold nanoparticle has absorption peak at 500-600nm.
The method of nano materials of the present invention has the advantages that following significant compared to other methods: (1) template is closed
There is comparable flexibility at nano material.(2) experimental provision is simple, and operating condition is mild.(3) a nanometer material can accurately be controlled
Size, pattern and the structure of material.(4) generation of nano material agglomeration can be prevented, so as to cause extensive concern.So
And polypeptide is also a kind of biological template of good performance, oneself knows that existing protein sum exceedes million kinds in entire living nature.Egg
White matter is the long-chain biological macromolecular being linked to be by several amino acid by peptide bond.Almost all most basic biologies in organism
Activity is all related with protein.Protein is rich in through functional groups such as base, amino, phosphate radicals, has very strong identification
Effect and good skeleton structure.
Claims (6)
1. a kind of method for preparing nano Au particle as template using Daptomycin, characterized by comprising the steps of: Yi Datuo
Mycin aqueous solution is template, and chlorauric acid solution is presoma, two kinds of solution is mixed with out mixed solution in proportion, heating is incubated
The combination for making the active site and presoma of template is educated, addition reducing agent is reacted, and the solution containing nano Au particle is made.
2. a kind of method for preparing nano Au particle as template using Daptomycin according to claim 1, it is characterised in that
It comprises the following steps: Daptomycin aqueous solution is adjusted into pH=1~13；It is mixed in proportion with chlorauric acid solution, puts it into gold
Belong in bath, is incubated for 0~48 hour at 50~1000 revs/min, 0 DEG C~80 DEG C；Reducing agent is added dropwise in solution after being incubated for, until
Solution colour no longer changes, and nano Au particle solution is made.
3. a kind of method for preparing nano Au particle as template using Daptomycin according to claim 1 or 2, feature exist
In the Daptomycin aqueous solution is configured using Daptomycin and deionized water according to mass ratio 1:333~10000；It is mould up to holding in the palm
Plain solution and chlorauric acid solution are the ratio mixing of 0.05~20:1 in molar ratio.
4. a kind of method for preparing nano Au particle as template using Daptomycin according to claim 2, which is characterized in that
To pH=1~13 of Daptomycin aqueous solution hydrochloric acid and sodium hydrate regulator solution.
5. a kind of method for preparing nano Au particle as template using Daptomycin according to claim 1 or 2, feature exist
In the reducing agent is dimethyamine borane or sodium borohydride.
6. a kind of method for preparing nano Au particle as template using Daptomycin according to claim 2, which is characterized in that
The condition that the metal bath is incubated for process is to be incubated for 0~48 hour at 50~1000 revs/min, 0 DEG C~80 DEG C.
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| CN201810948948.8A CN108941607A (en) | 2018-08-20 | 2018-08-20 | A method of nano Au particle is prepared by template of Daptomycin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109604632A (en) * | 2018-12-21 | 2019-04-12 | 燕山大学 | The method for preparing nano Au particle as template using polymyxin e |
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| US20130123466A1 (en) * | 2011-11-14 | 2013-05-16 | Gwangju Institute Of Science And Technology | Simultaneous synthesis of temperature-tunable peptide and gold nanoparticle hybrid spheres |
| CN103264987A (en) * | 2013-04-22 | 2013-08-28 | 浙江师范大学 | Gold nanocluster preparation method and application thereof |
| CN106085420A (en) * | 2016-06-02 | 2016-11-09 | 中国石油大学(华东) | A kind of peptide modified gold nanoclusters and preparation method thereof |
| CN106825600A (en) * | 2016-11-30 | 2017-06-13 | 燕山大学 | A kind of synthetic method of the starlike golden nanometer particle of photosensitivity |
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2018
- 2018-08-20 CN CN201810948948.8A patent/CN108941607A/en not_active Withdrawn
Patent Citations (4)
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|---|---|---|---|---|
| US20130123466A1 (en) * | 2011-11-14 | 2013-05-16 | Gwangju Institute Of Science And Technology | Simultaneous synthesis of temperature-tunable peptide and gold nanoparticle hybrid spheres |
| CN103264987A (en) * | 2013-04-22 | 2013-08-28 | 浙江师范大学 | Gold nanocluster preparation method and application thereof |
| CN106085420A (en) * | 2016-06-02 | 2016-11-09 | 中国石油大学(华东) | A kind of peptide modified gold nanoclusters and preparation method thereof |
| CN106825600A (en) * | 2016-11-30 | 2017-06-13 | 燕山大学 | A kind of synthetic method of the starlike golden nanometer particle of photosensitivity |
Non-Patent Citations (1)
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| 刘素美等: "丝蛋白多肽原位制备金纳米粒子的研究", 《贵金属》 * |
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| CN109604632A (en) * | 2018-12-21 | 2019-04-12 | 燕山大学 | The method for preparing nano Au particle as template using polymyxin e |
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