CN108939174B - 一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜及其制备方法 - Google Patents

一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜及其制备方法 Download PDF

Info

Publication number
CN108939174B
CN108939174B CN201810779074.8A CN201810779074A CN108939174B CN 108939174 B CN108939174 B CN 108939174B CN 201810779074 A CN201810779074 A CN 201810779074A CN 108939174 B CN108939174 B CN 108939174B
Authority
CN
China
Prior art keywords
layer
polyoxazoline
multilayer film
solution
oxazoline
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201810779074.8A
Other languages
English (en)
Other versions
CN108939174A (zh
Inventor
王征科
鲍晓炯
黄晓飞
金晓强
乔丰慧
傅倍佳
胡巧玲
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang University ZJU
Original Assignee
Zhejiang University ZJU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang University ZJU filed Critical Zhejiang University ZJU
Priority to CN201810779074.8A priority Critical patent/CN108939174B/zh
Publication of CN108939174A publication Critical patent/CN108939174A/zh
Application granted granted Critical
Publication of CN108939174B publication Critical patent/CN108939174B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/08—Materials for coatings
    • A61L31/082—Inorganic materials
    • A61L31/088—Other specific inorganic materials not covered by A61L31/084 or A61L31/086
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/08—Materials for coatings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/08—Materials for coatings
    • A61L31/10—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L31/16—Biologically active materials, e.g. therapeutic substances
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G73/00—Macromolecular compounds obtained by reactions forming a linkage containing nitrogen with or without oxygen or carbon in the main chain of the macromolecule, not provided for in groups C08G12/00 - C08G71/00
    • C08G73/06—Polycondensates having nitrogen-containing heterocyclic rings in the main chain of the macromolecule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
    • A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
    • A61L2300/104—Silver, e.g. silver sulfadiazine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2420/00—Materials or methods for coatings medical devices
    • A61L2420/02—Methods for coating medical devices
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2420/00—Materials or methods for coatings medical devices
    • A61L2420/06—Coatings containing a mixture of two or more compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2420/00—Materials or methods for coatings medical devices
    • A61L2420/08—Coatings comprising two or more layers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Vascular Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Surgery (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Materials For Medical Uses (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Laminated Bodies (AREA)
  • Medicinal Preparation (AREA)

Abstract

本发明公开了一种pH响应型聚噁唑啉‑纳米银的层层自组装多层膜及其制备方法，该自组装膜具有抗粘附作用，并具有pH响应性，从而实现抗菌剂纳米银的可控释放。本发明首先制备了功能性单体：2‑(2‑甲氧羰基)乙基‑2‑噁唑啉，然后通过共聚合反应制得了带负电荷的阴离子型聚噁唑啉(POx)；同时，利用儿茶酚接枝壳聚糖还原银离子，获得纳米银复合溶胶(CCS‑AgNPs)；最后利用静电层层自组装法，将带负电荷的聚噁唑啉和带正电荷的纳米银交替组装制得一种具有pH响应性的多层膜，该多层膜可以抵抗细菌粘附，在微酸pH刺激下实现抗菌剂纳米银的释放从而杀灭细菌，可实现抗‑杀一体化。

Description

一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜及其制 备方法
技术领域
本发明属于医用抗菌膜领域，具体涉及一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜及其制备方法。
背景技术
近几十年，由于心血管和各类代谢疾病的高发，进行医疗器械植入手术的患者日益增多。然而器械在植入体内的过程中，极易被细菌粘附在表面，形成一种细菌聚集体的膜状物，即细菌生物被膜。这种细菌感染已然成为植入手术失败的主要原因之一。因为器械表面的生物膜一旦形成，就极难被杀死和清除。相比于游离态细菌，膜内细菌对抗生素和抗菌剂的抗性大大增强，同时难以被巨噬细胞所吞噬，也无法被机体的免疫系统所清除，最终导致植入部位发生持续性的细菌感染。
许多研究结果表明，单一的抗粘附表面无法在体内长期抵抗生物膜的形成。因为细菌会不断分泌出大量粘结物，试图附着于器械表面。一旦抗粘附层被这种粘性极强的蛋白突破，细菌就能成功在蛋白上定植，形成大量菌落。由此可见，想要在临床应用中成功抵抗生物膜的形成,构筑单一功能的表面是不够的。将抗细菌粘附与杀菌结合起来，构筑一种新型的智能抗-杀表面，即具有细菌刺激响应性的表面，是一种潜在有效的策略。
已知细菌感染宿主细胞后会释放一系列致病因子，如磷酸酶、磷脂酶、毒素、脂肪酶、蛋白酶等，还会使感染部位周围的pH降低，发生较明显的酸化，形成一个特殊的细菌感染的微环境。这些微环境的变化，可以作为杀菌材料特异性释放或发挥触杀作用的信号源。因此，当这种智能抗-杀材料最外侧的抗细菌粘附层，在细菌的持续作用下,失去抗粘附性能后。附着于表面的细菌会使微环境发生变化，进而触发了杀菌剂的释放,特异性的杀死附着的细菌，防止生物膜的形成。在现有的涂层制备方法中，静电层层自组装技术(LBL)显然很适用于制备这种刺激响应性的抗-杀表面。其原理是利用静电相互作用将带有相反电荷的聚电解质交替沉积形成多层膜，静电自组装得到的多层膜可具备pH响应性。
聚噁唑啉(POx)作为一种类多肽结构的聚合物，具有生物相容性好，细胞毒性低，亲水性极强，能够抗蛋白粘附等优点；纳米银作为一种常见的抗菌剂，具有广谱高效的抗菌性能，且不易产生耐药性。带负电荷的POx，通过静电层层自组装将带正电荷的纳米银衍生物(CSS-AgNPs)负载到多层膜中，成功构筑一种新型的智能抗-杀表面，具有pH刺激响应性。
发明内容
本发明的目的是克服现有顽固的细菌生物被膜难以清除的问题，提供一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜及其制备方法，通过聚噁唑啉亲水表面抵抗细菌粘附，在微酸pH刺激下实现抗菌剂纳米银的释放从而杀灭细菌，实现抗-杀一体化。
本发明通过以下技术方案实现：首先制备了功能性单体：2-(2-甲氧羰基)乙基-2-噁唑啉，然后通过均聚合和共聚合反应制得了带负电荷的阴离子型聚噁唑啉；同时，利用儿茶酚接枝壳聚糖还原银离子获得纳米银复合溶胶；最后在起始层上利用静电层层自组装法，将带负电荷的聚噁唑啉和带正电荷的纳米银交替组装制得一种具有pH响应性的多层膜，该自组装膜具有抗粘附作用，并能可控释放抗菌剂纳米银。
上述技术方案中，所述的功能性单体2-(2-甲氧羰基)乙基-2-噁唑啉的制备步骤如下：
(1)首先通过丁二酸酐开环反应，得到中间产物丁二酸单甲酯；
(2)然后通过酰胺化反应得到黄色粘稠液体状粗产物；
(3)最后通过噁唑啉关环反应，可得淡黄色粉末状固体，即为目标产物：2-(2-甲氧羰基)乙基-2-噁唑啉。
其具体的制备过程可参考文献(A.Levy and M.Litt,Journal of PolymerScience:Part A-1,Vol.6,1883-1894(1968))，其反应过程如图1所示。
所述的共聚合的具体步骤如下：
(1)通过单体2-(2-甲氧羰基)乙基-2-噁唑啉和共单体2-乙基-2-噁唑啉的共聚合得到无规共聚物P2；
(2)再通过碱溶水解成盐，然后酸处理进行修饰，可得带负电的后修饰产物PT2。其反应过程如图2所示。
由此获得一个组装单层材料，即带负电荷的聚噁唑啉。优选的，单体2-(2-甲氧羰基)乙基-2-噁唑啉和共单体2-乙基-2-噁唑啉的共聚投料比例为：1:1～1:9。
另一组装单层材料：带正电荷的儿茶酚接枝壳聚糖-纳米银是通过酰胺化反应将3-(2,4-二羟基)苯基丙酸与壳聚糖上的氨基进行缩合得到的，其水溶性大大提高，并加入了纳米银。其制备方法如下：分别配制质量浓度为1～3％的儿茶酚接枝壳聚糖的水溶液和质量浓度为20～30％的硝酸银水溶液，在搅拌下，将硝酸银水溶液加入到儿茶酚接枝壳聚糖溶液中，反应1～5h后制得儿茶酚接枝壳聚糖-纳米银溶胶。
上述技术方案中，所述的起始层为支化聚乙烯亚胺(BPEI)。
本发明所述的pH响应型聚噁唑啉-纳米银的层层自组装膜的制备方法，具体步骤如下：
1)分别配制如下溶液：PBS缓冲液，BPEI溶液，CSS-AgNPs待用；并将制得的PT2配成水溶液得到POx溶液；
2)将玻片浸泡在BPEI溶液中进行预组装，至少20min后取出，用PBS缓冲液重复冲洗并吹干多次；
3)再将玻片浸泡到CSS-AgNPs溶液中，至少20min后取出，用PBS缓冲液重复冲洗并吹干多次；
4)再将玻片浸泡到POx溶液中，至少20min后取出，用PBS缓冲液重复冲洗并吹干多次；
5)重复上述步骤3)和步骤4)交替浸泡组装，直至达到要求的组装层数，且聚噁唑啉层位于最外层为止。
得到的pH响应型聚噁唑啉-纳米银的层层自组装膜，具有抗粘附作用，并能可控释放抗菌剂，其机理图如图3所示。
本发明相对于现有技术具有以下优点：
1)本发明选择纳米银作为抗菌剂，具有广谱高效的抗菌性能，细胞毒性小，水溶性佳。
2)本发明选择聚噁唑啉作为亲水抗粘附表面组装层，生物相容性好，细胞毒性低，亲水性极强，能够抗蛋白粘附等，且相比于PEG，它的分子链柔性更强、端基更易于功能化、亲水性可调。
3)本发明具有pH刺激响应性，可在正常体液pH时保持亲水表面，抵抗细菌粘附；在微酸pH时，实现抗菌剂纳米银的可控释放。本发明实现了抗-杀一体化。
附图说明
图1是功能性单体2-(2-甲氧羰基)乙基-2-噁唑啉的制备过程原理图；
图2是共聚合过程原理图；
图3是本发明多层膜的结构示意图及机理图。
图4为组装膜的接触角随组装层数的变化。接触角随最外层组装膜的亲疏水性交错变化，当聚噁唑啉在最外层时，亲水性更强。
图5为pH＝5.5,6.5,7.4时银的释放百分比随释放时间的变化。pH＝7.4时，银基本不释放；pH＝6.5时，10分钟达到极限释放百分比约10％；pH＝5.5时，10分钟达到极限释放百分比约40％。
具体实施方式
以下结合实例进一步说明本发明。
实施例1：
1)配制如下浓度的溶液待用：PBS 0.01M，BPEI 3mg/mL，CSS-AgNPs 1mg/mL，POx1mg/mL。
2)将玻片浸泡在BPEI溶液中进行预组装，30min后取出，用PBS缓冲液重复冲洗并吹干三次。
3)将玻片浸泡到CSS-AgNPs溶液中，30min后取出，用PBS缓冲液重复冲洗并吹干三次。
4)将玻片浸泡到POx溶液中，30min后取出，用PBS缓冲液重复冲洗并吹干三次。
5)重复交替浸泡组装，直至达到要求的组装层数为止。
实施例2：
1)配制如下浓度的溶液待用：PBS 0.01M，BPEI 2mg/mL，CSS-AgNPs 2mg/mL，POx2mg/mL。
2)将玻片浸泡在BPEI溶液中进行预组装，30min后取出，用PBS缓冲液重复冲洗并吹干三次。
3)将玻片浸泡到CSS-AgNPs溶液中，30min后取出，用PBS缓冲液重复冲洗并吹干三次。
4)将玻片浸泡到POx溶液中，30min后取出，用PBS缓冲液重复冲洗并吹干三次。
5)重复交替浸泡组装，直至达到要求的组装层数为止。
实施例3：
1)配制如下浓度的溶液待用：PBS 0.01M，BPEI 2mg/mL，CSS-AgNPs 1mg/mL，POx1mg/mL。
2)将玻片浸泡在BPEI溶液中进行预组装，30min后取出，用PBS缓冲液重复冲洗并吹干三次。
3)将玻片浸泡到CSS-AgNPs溶液中，30min后取出，用PBS缓冲液重复冲洗并吹干三次。
4)将玻片浸泡到POx溶液中，30min后取出，用PBS缓冲液重复冲洗并吹干三次。
5)重复交替浸泡组装，直至达到要求的组装层数为止。

Claims (7)

1.一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜，其特征在于：首先制备了功能性单体：2-(2-甲氧羰基)乙基-2-噁唑啉，然后通过共聚合反应制得了带负电荷的阴离子型聚噁唑啉；同时，利用儿茶酚接枝壳聚糖还原银离子，获得纳米银复合溶胶(CSS-AgNPs)；最后在起始层上利用静电层层自组装法，将带负电荷的聚噁唑啉和带正电荷的纳米银交替组装，制得一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜。
2.如权利要求1所述的pH响应型聚噁唑啉-纳米银的层层自组装多层膜，其特征在于：所述的功能性单体：2-(2-甲氧羰基)乙基-2-噁唑啉的制备步骤如下：(1)首先通过丁二酸酐开环反应，得到中间产物丁二酸单甲酯；(2)然后通过酰胺化反应得到黄色粘稠液体状粗产物；(3)最后通过噁唑啉关环反应，可得淡黄色粉末状固体，即为目标产物：2-(2-甲氧羰基)乙基-2-噁唑啉。
3.如权利要求1所述的pH响应型聚噁唑啉-纳米银的层层自组装多层膜，其特征在于：所述的共聚合的具体步骤如下：(1)通过单体2-(2-甲氧羰基)乙基-2-噁唑啉和共单体：2-乙基-2-噁唑啉的共聚合得到无规共聚物P2；(2)通过碱溶水解成盐，再酸处理进行修饰，可得带负电的后修饰产物PT2。
4.如权利要求3所述的pH响应型聚噁唑啉-纳米银的层层自组装多层膜，其特征在于：所述的单体2-(2-甲氧羰基)乙基-2-噁唑啉和共单体：2-乙基-2-噁唑啉的共聚投料比例为：1:1～1:9。
5.如权利要求1所述的pH响应型聚噁唑啉-纳米银的层层自组装多层膜，其特征在于：所述的纳米银复合溶胶的制备方法如下：分别配制质量浓度为1～3％的儿茶酚接枝壳聚糖的水溶液和质量浓度为20～30％的硝酸银水溶液，在搅拌下，将硝酸银水溶液加入到儿茶酚接枝壳聚糖溶液中，反应1～5h后制得儿茶酚接枝壳聚糖-纳米银溶胶。
6.如权利要求1所述的pH响应型聚噁唑啉-纳米银的层层自组装多层膜，其特征在于：所述的起始层为支化聚乙烯亚胺(BPEI)。
7.如权利要求1-6任一项所述的一种pH响应型聚噁唑啉-纳米银的层层自组装膜的制备方法，其特征在于步骤如下：
1)分别配制如下溶液：PBS缓冲液，BPEI溶液，CSS-AgNPs待用；并将制得的PT2配成水溶液得到POx溶液；
2)将玻片浸泡在BPEI溶液中进行预组装，至少20min后取出，用PBS缓冲液重复冲洗并吹干多次；
3)再将玻片浸泡到CSS-AgNPs溶液中，至少20min后取出，用PBS缓冲液重复冲洗并吹干多次；
4)再将玻片浸泡到POx溶液中，至少20min后取出，用PBS缓冲液重复冲洗并吹干多次；
5)重复上述步骤3)和步骤4)交替浸泡组装，直至达到要求的组装层数，且聚噁唑啉层位于最外层为止。
CN201810779074.8A 2018-07-16 2018-07-16 一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜及其制备方法 Active CN108939174B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810779074.8A CN108939174B (zh) 2018-07-16 2018-07-16 一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜及其制备方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810779074.8A CN108939174B (zh) 2018-07-16 2018-07-16 一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜及其制备方法

Publications (2)

Publication Number Publication Date
CN108939174A CN108939174A (zh) 2018-12-07
CN108939174B true CN108939174B (zh) 2020-06-19

Family

ID=64481677

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810779074.8A Active CN108939174B (zh) 2018-07-16 2018-07-16 一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜及其制备方法

Country Status (1)

Country Link
CN (1) CN108939174B (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116808302A (zh) * 2023-06-30 2023-09-29 征鸿诺瓦医疗科技(深圳)有限公司 一种基体表面层层静电组装涂层的制备方法及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2563246T3 (es) * 2012-03-16 2016-03-11 Bender Analytical Holding B.V. Polímeros reticulados y productos médicos derivados de polioxazolina nucleofílicamente activada
CN107106719B (zh) * 2014-10-06 2021-06-01 加特技术公司 组织粘合性多孔止血产品
CN105478792B (zh) * 2015-12-05 2017-08-04 浙江大学 一种改性壳聚糖‑纳米银溶胶的绿色制备方法

Also Published As

Publication number Publication date
CN108939174A (zh) 2018-12-07

Similar Documents

Publication Publication Date Title
Zhao et al. Antibacterial hydrogel coating: Strategies in surface chemistry
Mitra et al. Polymer-based coatings with integrated antifouling and bactericidal properties for targeted biomedical applications
Qiu et al. The mechanisms and the applications of antibacterial polymers in surface modification on medical devices
Stojanov et al. Electrospun nanofibers as carriers of microorganisms, stem cells, proteins, and nucleic acids in therapeutic and other applications
Yan et al. Nonleaching bacteria-responsive antibacterial surface based on a unique hierarchical architecture
Ahmed et al. Adaptive antibacterial biomaterial surfaces and their applications
Kolewe et al. Fewer bacteria adhere to softer hydrogels
Yang et al. Smart antibacterial surface made by photopolymerization
You et al. Versatile polymer-based strategies for antibacterial drug delivery systems and antibacterial coatings
Vendra et al. Polymer thin films for biomedical applications
Wang et al. Inhibition of Escherichia coli and Proteus mirabilis adhesion and biofilm formation on medical grade silicone surface
Qian et al. Biomimetic domain-active electrospun scaffolds facilitating bone regeneration synergistically with antibacterial efficacy for bone defects
Fu et al. Recent advances in hydrogel-based anti-infective coatings
Li et al. Electroactive materials: Innovative antibacterial platforms for biomedical applications
Ayaz et al. A pH and hyaluronidase dual-responsive multilayer-based drug delivery system for resisting bacterial infection
Wu et al. Acidity-triggered charge-reversible multilayers for construction of adaptive surfaces with switchable bactericidal and bacteria-repelling functions
Li et al. Chemical grafting of antibiotics into multilayer films through Schiff base reaction for self-defensive response to bacterial infections
CN109789244B (zh) 多肽和透明质酸涂层
Kolewe et al. Fouling-resistant hydrogels prepared by the swelling-assisted infusion and polymerization of dopamine
Ulusan et al. Biologically functional ultrathin films made of zwitterionic block copolymer micelles
Dhingra et al. Infection resistant surface coatings by polymer brushes: strategies to construct and applications
Qiao et al. Synergistic anti-inflammatory coating “Zipped Up” on polypropylene hernia mesh
Sun et al. Solvent-Free graft-from polymerization of Polyvinylpyrrolidone imparting ultralow bacterial fouling and improved biocompatibility
Chu et al. Recent advance in polymer coatings combating bacterial adhesion and biofilm formation
CN108939174B (zh) 一种pH响应型聚噁唑啉-纳米银的层层自组装多层膜及其制备方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CB03 Change of inventor or designer information
CB03 Change of inventor or designer information

Inventor after: Bao Xiaojiong

Inventor after: Huang Xiaofei

Inventor after: Jin Xiaoqiang

Inventor after: Qiao Fenghui

Inventor after: Fu Beijia

Inventor after: Hu Qiaoling

Inventor after: Wang Zhengke

Inventor before: Wang Zhengke

Inventor before: Bao Xiaojiong

Inventor before: Huang Xiaofei

Inventor before: Jin Xiaoqiang

Inventor before: Qiao Fenghui

Inventor before: Fu Beijia

Inventor before: Hu Qiaoling