CN108935743A - A kind of trans-fatty acid-free smears rouge and preparation method thereof - Google Patents
A kind of trans-fatty acid-free smears rouge and preparation method thereof Download PDFInfo
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- CN108935743A CN108935743A CN201810478966.4A CN201810478966A CN108935743A CN 108935743 A CN108935743 A CN 108935743A CN 201810478966 A CN201810478966 A CN 201810478966A CN 108935743 A CN108935743 A CN 108935743A
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- rouge
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- fatty acid
- free
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
- A23D9/04—Working-up
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- Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Fats And Perfumes (AREA)
- Edible Oils And Fats (AREA)
Abstract
The present invention relates to a kind of trans-fatty acid-frees to smear rouge and preparation method thereof, belongs to food technology field.The present invention is by extracting the soluble hydrocolloid in bamboo shoots, its apparent viscosity and moisture holding capacity with higher, the properties of energy simulated fat in food system, generate preferable lubrication sense, assign the good texture of food, reach partial fat in substitute food product system, and three-dimensional net structure is formed to make edible oil become solid plastic fat by self assembly mode with dialycerides, it can be used for preventing hyperlipidemia and its relevant cardiovascular disease, it can be used to pre- preventing obesity because that will not build up in vivo after intake, so by as functional food special fat, it is daily to food color to can satisfy people, it is fragrant, the needs of taste, and the eating habit of people can not be changed, reduce the intake of adipose energy, to effectively prevent and mitigate hyperlipidemia, the puzzlement of many chronic diseases such as obesity.
Description
Technical field
The present invention relates to a kind of trans-fatty acid-frees to smear rouge and preparation method thereof, belongs to food technology field.
Background technique
With being gradually increased for the living standard of China's rapid development of economy and people, diet consumption structure
Just occurring quickly to change, the cooking culture in west has obtained the approval of more and more people.Rouge is smeared as a kind of Western-style food
Product are quickly grown in China in recent years, especially in the developed regions of East Coastal, smear rouge also in large supermarket several years ago
Only very few several money products have gradually developed to tens of kinds now, and brand and specification are also to tend to diversification.As one kind
The healthy product of low fat smears rouge and is increasingly liked that the consumer group of especially some youths starts to be accustomed to by people
It is edible in smearing bread in breakfast.Meanwhile the trans-fatty acid problem for smearing rouge similarly annoyings us.
In addition, with the change of people's dietary structure, bake the food proportions such as class, fried class, extruding class it is continuous on
It rises, this also causes the demand of food special oil increasing.In order to meet consumers to delicious and health food
Demand actively researches and develops trans-fatty acid-free food special fat, has become weight current very urgent and with realistic meaning
Want project.
With the fast development of modern biotechnology, technique for gene engineering, function admirable, cheap lipase are obtained
Have become possibility, and the novel immobilized lipase researched and developed in recent years, have a wide range of application, activity is high, is converted into reality
The potentiality of application are bigger, this brings new opportunity to oils industry.Currently, many food of the developed countries such as American-European and Japan
The manufacturing enterprise of special oil is using the research of enzyme process ester-exchange technology as emphasis, gradually to replace the use of hydrogenated oil and fat.I
The technology and formula of state's food special oil mostly come from foreign countries, as smearing fat prod, are substantially all import to be relied on.And it is low or
The research of trans-fatty acid-free food special oil is also in the exploratory stage, and the Research foundation about bio-modification key technology is very
Weakness, food special oil Biotechnology are badly in need of breaking through.
With the quickening pace of modern life, the irregular phenomenon of the diet of people is more serious, this number is also on constantly
Rise.Simultaneously as people's nutrient knowledge is enriched constantly, deepen continuously to the relationship understanding between diet and disease, especially food
Quality, health, awareness of safety are continuously improved, and people are also being continuously increased the vigilance of edible fat.People are in order to pursue health
Body, will be increasing to the market of low calorie fat.Therefore, research and development fat substitute low calorie fat tool
There is important meaning.
Summary of the invention
The technical problems to be solved by the invention: for fixed product is carried out by crosslinking agent, although durability reaches
Desired effect, but since anchor effect is excessively good, the release of amino acid is affected instead, while the feel of final products receives
The problem of influence, provides a kind of trans-fatty acid-free and smears rouge and preparation method thereof.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
A kind of trans-fatty acid-free smearing rouge, including following weight parts raw material composition:
100~200g edible oil, 8~15g dialycerides, 5~10g bamboo shoot edible fiber.
The edible oil is one of soybean oil, camellia oil or palm oil or a variety of.
The dialycerides are stirred to react after melting for the heating of the mixture of stearic acid and glycerol with immobilized lipase, and
It is stood at 80~90 DEG C, take upper oil phase and is evaporated under reduced pressure removal low-boiling-point substance and is obtained.
The molar ratio of the stearic acid and glycerol is 1:8~1:9.
The immobilized lipase is Novozym435, and dosage is the 5% of mixture quality.
The process that is stirred to react is to be stirred to react 40~50min at 50~60 DEG C with 300~400r/min.
The bamboo shoot edible fiber is to be stirred at 50~60 DEG C in bamboo shoots powder addition deionized water, and divided with quality
Number is that 20% sodium bicarbonate solution adjusts pH to 8~9, adds amylase insulated and stirred, sterilizes and be centrifuged point after reaction
From drying is made after collecting precipitating and being washed to neutrality.
The bamboo shoots powder is to pulverize after drying after bamboo shoots are smashed to pieces to moisture content 10~15%.
The amylase is alpha-amylase, and dosage is the 2% of bamboo shoots silty amount.
A kind of trans-fatty acid-free smears the preparation method of rouge, specific steps are as follows:
(1) it pulverizes after drying after smashing bamboo shoots to pieces to moisture content 10~15%, obtains bamboo shoots powder;
(2) it takes bamboo shoots powder to be added in deionized water to be stirred at 50~60 DEG C, and is that 20% sodium bicarbonate is molten with mass fraction
Liquid adjusts pH to 8~9, adds amylase insulated and stirred, sterilizes and be centrifugated after reaction, collects and precipitates and be washed to
It is dry after neutrality, obtain bamboo shoot edible fiber;
(3) it will be stirred to react after the heating fusing of the mixture of stearic acid and glycerol with immobilized lipase, and at 80~90 DEG C
It stands, takes upper oil phase and be evaporated under reduced pressure removal low-boiling-point substance, obtain dialycerides;
(4) edible oil, dialycerides, bamboo shoot edible fiber stir 40~60min at 60~80 DEG C, stand after cooling and 0
It is saved at~4 DEG C, obtains trans-fatty acid-free and smear rouge.
The present invention is compared with other methods, and advantageous effects are:
(1) the soluble hydrocolloid of the invention by extracting in bamboo shoots, apparent viscosity and moisture holding capacity with higher,
The properties of energy simulated fat in food system, generate preferable lubrication sense, and the imparting good texture of food reaches substitution food
Partial fat in product system, and three-dimensional net structure is formed to make edible oil become solid by self assembly mode with dialycerides
The plastic fat of state can be used for preventing hyperlipidemia and its relevant cardiovascular disease, can because that will not build up in vivo after intake
For pre- preventing obesity, so by as functional food special fat;
(2) the fine and smooth uniform, ductility of product of the present invention crystal and tackness are preferable, close with commercial prod, and store up at 4 DEG C
It still is able to maintain stabilization after depositing 3 months, can satisfy the daily needs to food color of people, and people can not be changed
Eating habit, reduce the intake of adipose energy, thus effectively prevent and mitigate hyperlipidemia, many chronic diseases such as obesity
The puzzlement of disease.
Specific embodiment
Bamboo shoots are fitted into tissue mashing machine and are smashed to pieces, the bamboo shoots slurries after smashing to pieces are placed in drying box, at 60~80 DEG C
Lower drying to moisture content is 10~15%, then is transferred in mortar 20~40min of grinding, crosses 60 meshes, obtains bamboo shoots powder, take 500~
800g bamboo shoots powder is added in 3.0~4.8L deionized water, stirs 20~30min at 50~60 DEG C with 300~400r/min,
It is that 20% sodium bicarbonate solution adjusts pH to 8~9 with mass fraction, 10~16g amylase, 3~5h of insulated and stirred, reaction is added
After be fitted into high-pressure sterilizing pot, 10~15min of sterilization treatment at 120~125 DEG C, then be transferred in centrifuge with 6000~
8000r/min is centrifugated 20~30min, collects precipitating, is washed with deionized and is precipitated to neutrality, and does at 60~70 DEG C
It is dry to constant weight, obtain bamboo shoot edible fiber, 1:8~1:9 is mixed in molar ratio with glycerol by stearic acid, takes 500~800g mixture
It is fitted into reaction kettle, is heated to mixture and is completely melt, add 25~40g immobilized lipase, with 300 at 50~60 DEG C
~400r/min is stirred to react 40~50min, stands 1~2h at 80~90 DEG C after reaction, takes upper oil phase and depressurize
Distillation removal low-boiling-point substance, obtains dialycerides, takes 100~200g edible oil, 8~15g dialycerides, 5~10g bamboo shoots diet fibre
Dimension stirs 40~60min at 60~80 DEG C with 200~300r/min, and 24~36h is stood after being cooled to room temperature, is placed in cold
Freeze in case, saved at 0~4 DEG C, obtains trans-fatty acid-free and smear rouge.
Bamboo shoots are fitted into tissue mashing machine and are smashed to pieces, the bamboo shoots slurries after smashing to pieces are placed in drying box, are done at 60 DEG C
Dry to moisture content is 10%, then is transferred in mortar and grinds 20min, cross 60 meshes, obtain bamboo shoots powder, take 500g bamboo shoots powder, 3.0L is added
In deionized water, 20min is stirred with 300r/min at 50 DEG C, is that 20% sodium bicarbonate solution adjusts pH to 8 with mass fraction,
10g amylase is added, insulated and stirred 3h is fitted into high-pressure sterilizing pot after reaction, the sterilization treatment 10min at 120 DEG C, then
It is transferred in centrifuge and 20min is centrifugated with 6000r/min, collect precipitating, be washed with deionized and be precipitated to neutrality, and 60
It dries at DEG C to constant weight, obtains bamboo shoot edible fiber, 1:8 is mixed in molar ratio with glycerol by stearic acid, and 500g mixture is taken to be packed into
It in reaction kettle, is heated to mixture and is completely melt, add 25g immobilized lipase, be stirred to react at 50 DEG C with 30r/min
40min stands 1h at 80 DEG C after reaction, takes upper oil phase and is evaporated under reduced pressure removal low-boiling-point substance, obtains dialycerides, take
100g edible oil, 8g dialycerides, 5g bamboo shoot edible fiber stir 40min at 60 DEG C with 200r/min, after being cooled to room temperature
It stands for 24 hours, is placed in household freezer, is saved at 0 DEG C, obtain trans-fatty acid-free and smear rouge.
Bamboo shoots are fitted into tissue mashing machine and are smashed to pieces, the bamboo shoots slurries after smashing to pieces are placed in drying box, are done at 70 DEG C
Dry to moisture content is 12%, then is transferred in mortar and grinds 30min, cross 60 meshes, obtain bamboo shoots powder, take 600g bamboo shoots powder, 4L is added and goes
In ionized water, 25min is stirred with 360r/min at 55 DEG C, is that 20% sodium bicarbonate solution adjusts pH to 8.5 with mass fraction,
12g amylase is added, insulated and stirred 4h is fitted into high-pressure sterilizing pot after reaction, the sterilization treatment 12min at 120 DEG C, then
It is transferred in centrifuge and 25min is centrifugated with 7000r/min, collect precipitating, be washed with deionized and be precipitated to neutrality, and 65
It dries at DEG C to constant weight, obtains bamboo shoot edible fiber, 1:8 is mixed in molar ratio with glycerol by stearic acid, and 600g mixture is taken to be packed into
It in reaction kettle, is heated to mixture and is completely melt, add 30g immobilized lipase, it is anti-with 300r/min stirring at 55 DEG C
45min is answered, stands 1h at 85 DEG C after reaction, take upper oil phase and is evaporated under reduced pressure removal low-boiling-point substance, dialycerides is obtained, takes
150g edible oil, 10g dialycerides, 8g bamboo shoot edible fiber stir 50min at 70 DEG C with 240r/min, are cooled to room temperature
After stand 30h, be placed in household freezer, saved at 2 DEG C, obtain trans-fatty acid-free smear rouge.
Bamboo shoots are fitted into tissue mashing machine and are smashed to pieces, the bamboo shoots slurries after smashing to pieces are placed in drying box, are done at 80 DEG C
Dry to moisture content is 15%, then is transferred in mortar and grinds 40min, cross 60 meshes, obtain bamboo shoots powder, take 800g bamboo shoots powder, 4.8L is added
In deionized water, 30min is stirred with 400r/min at 60 DEG C, is that 20% sodium bicarbonate solution adjusts pH to 9 with mass fraction,
16g amylase is added, insulated and stirred 5h is fitted into high-pressure sterilizing pot after reaction, the sterilization treatment 15min at 125 DEG C, then
It is transferred in centrifuge and 30min is centrifugated with 8000r/min, collect precipitating, be washed with deionized and be precipitated to neutrality, and 70
It dries at DEG C to constant weight, obtains bamboo shoot edible fiber, 1:9 is mixed in molar ratio with glycerol by stearic acid, and 800g mixture is taken to be packed into
It in reaction kettle, is heated to mixture and is completely melt, add 40g immobilized lipase, it is anti-with 400r/min stirring at 60 DEG C
50min is answered, stands 2h at 90 DEG C after reaction, take upper oil phase and is evaporated under reduced pressure removal low-boiling-point substance, dialycerides is obtained, takes
200g edible oil, 15g dialycerides, 10g bamboo shoot edible fiber stir 60min at 80 DEG C with 200~300r/min, cooling
36h is stood after to room temperature, is placed in household freezer, is saved at 4 DEG C, trans-fatty acid-free is obtained and smears rouge.
The smearing rouge that trans-fatty acid-free prepared by the present invention smears rouge and the production of Guangzhou company is detected, specifically
Testing result such as following table table 1:
(1) solid fats content (SFC) measurement of rouge is smeared: using the hard fat of low field pulse nmr determination sample
Content.
Smear the determination of physical appearance of rouge: holding oiliness (OBC) is used to characterize the ability of combination.
The smearing rouge for taking 1mL to melt weighs gross weight after 4 DEG C of refrigerators keep 1h in the 10mL centrifuge tube for having weighed (a),
It is denoted as b.Then 15min is centrifuged with 9 × 103r/min, stands and draw the smearing rouge being precipitated and weigh remaining sample quality, is denoted as
c.OBC=(1-(b-c)/(b-a)) × 100% crystallization formed time (CFT) be used to characterize smear rouge formed gel energy
Power.It takes 1mL to smear rouge in 2mL centrifuge tube, 1h is kept under the conditions of 90 DEG C to eliminate crystallization memory, then takes out and is placed in room temperature
Under, it is inverted centrifuge tube, starts timing, the time required to record sample solidifies completely.
(3) stability (MS) is used to characterize the mechanical stability for smearing rouge.1mL is taken to smear rouge in 2mL centrifuge tube, 4
DEG C refrigerator takes out after placing 12h, and 1.3 × 103r/min is centrifuged 15min.If thering is edible oil precipitation to illustrate that smearing rouge is unstable, mark
It is denoted as "-", otherwise is to stablize, is labeled as "+".
1 trans-fatty acid-free of table smears rouge performance characterization
Trans-fatty acid-free smearing rouge stability prepared by the present invention, holding oiliness are good as shown in Table 1, can wrap up more liquid
Oil, to make that product surface is smooth, texture is soft, delicate mouthfeel.
Claims (10)
1. a kind of trans-fatty acid-free smears rouge, which is characterized in that formed including following weight parts raw material:
100~200g edible oil, 8~15g dialycerides, 5~10g bamboo shoot edible fiber.
2. a kind of trans-fatty acid-free as described in claim 1 smears rouge, which is characterized in that the edible oil be soybean oil,
One of camellia oil or palm oil are a variety of.
3. a kind of trans-fatty acid-free as described in claim 1 smears rouge, which is characterized in that the dialycerides are stearic acid
It is stirred to react with after the mixture heating fusing of glycerol with immobilized lipase, and is stood at 80~90 DEG C, take upper oil phase
And it is evaporated under reduced pressure removal low-boiling-point substance and is made.
4. a kind of trans-fatty acid-free as claimed in claim 3 smears rouge, which is characterized in that the stearic acid and glycerol rub
You are than being 1:8~1:9.
5. a kind of trans-fatty acid-free as claimed in claim 3 smears rouge, which is characterized in that the immobilized lipase is
Novozym435, dosage are the 5% of mixture quality.
6. a kind of trans-fatty acid-free as claimed in claim 3 smears rouge, which is characterized in that it is described be stirred to react process be
40~50min is stirred to react with 300~400r/min at 50~60 DEG C.
7. a kind of trans-fatty acid-free as described in claim 1 smears rouge, which is characterized in that the bamboo shoot edible fiber is bamboo
Bamboo shoot powder is added in deionized water and is stirred at 50~60 DEG C, and is that 20% sodium bicarbonate solution adjusts pH to 8 with mass fraction
~9, amylase insulated and stirred is added, sterilizes and is centrifugated after reaction, is collected dry after precipitating and being washed to neutrality
It is made.
8. a kind of trans-fatty acid-free as claimed in claim 7 smears rouge, which is characterized in that the bamboo shoots powder is smashed to pieces for bamboo shoots
It pulverizes after drying afterwards to moisture content 10~15%.
9. a kind of trans-fatty acid-free as claimed in claim 7 smears rouge, which is characterized in that the amylase is alphalise starch
Enzyme, dosage are the 2% of bamboo shoots silty amount.
10. a kind of trans-fatty acid-free smears the preparation method of rouge as claimed in any one of claims 1 to 9 wherein, feature exists
In specific steps are as follows:
(1) it pulverizes after drying after smashing bamboo shoots to pieces to moisture content 10~15%, obtains bamboo shoots powder;
(2) it takes bamboo shoots powder to be added in deionized water to be stirred at 50~60 DEG C, and is that 20% sodium bicarbonate is molten with mass fraction
Liquid adjusts pH to 8~9, adds amylase insulated and stirred, sterilizes and be centrifugated after reaction, collects and precipitates and be washed to
It is dry after neutrality, obtain bamboo shoot edible fiber;
(3) it will be stirred to react after the heating fusing of the mixture of stearic acid and glycerol with immobilized lipase, and at 80~90 DEG C
It stands, takes upper oil phase and be evaporated under reduced pressure removal low-boiling-point substance, obtain dialycerides;
(4) edible oil, dialycerides, bamboo shoot edible fiber stir 40~60min at 60~80 DEG C, stand after cooling and 0
It is saved at~4 DEG C, obtains trans-fatty acid-free and smear rouge.
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CN102892301A (en) * | 2010-05-14 | 2013-01-23 | 邦奇油类公司 | Shortening compositions and methods of making and using the same |
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CN103361387A (en) * | 2013-07-25 | 2013-10-23 | 华南理工大学 | Production method for coproducing unsaturated monoglyceride by using diglyceride enzyme method |
CN104799207A (en) * | 2014-01-23 | 2015-07-29 | 天津亨天利化学有限公司 | Bamboo shoot offcut dietary fiber powder preparation method |
CN106480116A (en) * | 2016-06-08 | 2017-03-08 | 江南大学 | A kind of method of enzymatic clarification 1,3 distearyl acid 2 oleins |
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