CN108931511A - The fluorescence detection device and method of ultraviolet LED - Google Patents
The fluorescence detection device and method of ultraviolet LED Download PDFInfo
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- CN108931511A CN108931511A CN201810563113.0A CN201810563113A CN108931511A CN 108931511 A CN108931511 A CN 108931511A CN 201810563113 A CN201810563113 A CN 201810563113A CN 108931511 A CN108931511 A CN 108931511A
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 17
- 230000005284 excitation Effects 0.000 claims abstract description 54
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000010586 diagram Methods 0.000 description 3
- 229930195730 Aflatoxin Natural products 0.000 description 2
- 239000005409 aflatoxin Substances 0.000 description 2
- 210000001367 artery Anatomy 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 206010037660 Pyrexia Diseases 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000002211 ultraviolet spectrum Methods 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6423—Spectral mapping, video display
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06146—Multisources for homogeneisation, as well sequential as simultaneous operation
- G01N2201/06153—Multisources for homogeneisation, as well sequential as simultaneous operation the sources being LED's
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
- G01N2201/0625—Modulated LED
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/069—Supply of sources
- G01N2201/0696—Pulsed
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- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention relates to a kind of fluorescence detection device of ultraviolet LED and methods, it includes for placing the sample placement rack to fluorescence detection sample and being used to support the sample rack of the sample placement rack, the fluorescent light source of excitation light source and the image acquisition device for acquiring image are also provided as on the sample rack, the fluorescent light source is located at the surface of sample placement rack, and the ultraviolet light of fluorescent light source transmitting, which can be aligned, to be radiated on sample placement rack；It further include the drive electric power unit for driving fluorescent light source, the drive electric power unit can control the luminous frequency of fluorescent light source, and the luminous frequency of fluorescent light source and the frequency of exposure ratio of image acquisition device is made to be positive integer times, to reduce the stray light in fluorescence detection.Structure of the invention is compact, can effectively reduce the influence of stray light in fluorescence detection, improves detection efficiency, securely and reliably.
Description
Technical field
The present invention relates to a kind of fluorescence detection device and method, the fluorescence detection device of especially a kind of ultraviolet LED and side
Method belongs to the technical field of fluorescence detection.
Background technique
In the prior art, early stage ultraviolet spectrometer majority uses low pressure mercury lamp for light source, or using the arteries and veins lasted a long time
Xenon lamp is rushed, but all there is the disadvantages of pollution, volume is big, energy consumption is high and fever is serious.With the development of science and technology, at present
Start to issue using small molecule OLED light source corresponding using small in size, the excitation light source of LED light source at low cost as detector
The exciting light of wavelength and some strength is used for fluorescence detection.Use UVLED as excitation light source with certain spoke in addition, also having
It penetrates power, certain spectral region, certain illumination frequency and issues food of the ultraviolet light irradiation containing aflatoxins, inspire glimmering
Light, to achieve the purpose that detect aflatoxins.But when UV LED is as fluorescence excitaton source, output power is small, bandwidth and color
Temperature is also unstable, causes the underpower of UV-LED and the stray light of fluorescent image.
Summary of the invention
The purpose of the present invention is overcoming the deficiencies in the prior art, a kind of fluorescence detection device of ultraviolet LED is provided
And method, it is compact-sized, the influence of stray light in fluorescence detection can be effectively reduced, improves detection efficiency, securely and reliably.
According to technical solution provided by the invention, the fluorescence detection device of the ultraviolet LED, including for placing to fluorescence
The sample placement rack of test sample and the sample rack for being used to support the sample placement rack, also set on the sample rack
It sets as the fluorescent light source of excitation light source and the image acquisition device for acquiring image, the fluorescent light source is located at sample placement
The surface of seat, the ultraviolet light of fluorescent light source transmitting, which can be aligned, to be radiated on sample placement rack；
It further include the drive electric power unit for driving fluorescent light source, the drive electric power unit can control fluorescent light source
Luminous frequency, and the luminous frequency of fluorescent light source and the frequency of exposure ratio of image acquisition device is made to be positive integer times, to reduce
Stray light in fluorescence detection.
Described image collector includes the colour TV camera with CCD acquisition memory, and image acquisition device is also and with figure
As the calculating mechatronics of processing capacity.
The drive electric power unit includes driving power for connecting with fluorescence power supply power end and drives for controlling
The impulse generator of dynamic power supply power supply frequency.
The drive electric power unit includes driving power for connecting with fluorescence power supply power end and drives for controlling
The impulse generator of dynamic power supply power supply frequency, the impulse generator are connect with computer, and computer capacity controls impulse generator
Pulse generation frequency and amplitude.
The fluorescent light source includes several excitation lighting sources being uniformly distributed in lampshade, the excitation lighting source packet
It includes as the ultraviolet LED group of excitation light source and for the white LED light source as lighting source；
The ultraviolet LED group include mutually independent first ultraviolet LED excitation light source, the second ultraviolet LED excitation light source with
And third ultraviolet LED excitation light source, the first ultraviolet LED excitation light source emitting ultraviolet light line wavelength be 200nm~285nm, second
Laser LED light source (13) emitting ultraviolet light line wavelength is 365nm~385nm, and third ultraviolet LED excitation light source emits ultraviolet light wave
A length of 405nm.
Light source collector bracket is set on the sample rack, and fluorescent light source and image acquisition device are assemblied in the light
On source collector bracket.
A kind of fluorescence detection method of ultraviolet LED, including for place to fluorescence detection sample sample placement rack and
It is used to support the sample rack of the sample placement rack, the fluorescent light source of excitation light source is also provided as on the sample rack
And the image acquisition device for acquiring image, the fluorescent light source are located at the surface of sample placement rack, fluorescent light source transmitting
Ultraviolet light can be aligned and be radiated on sample placement rack；
It further include the drive electric power unit for driving fluorescent light source, the drive electric power unit can control fluorescent light source
Luminous frequency, and the luminous frequency of fluorescent light source and the frequency of exposure ratio of image acquisition device is made to be positive integer times, to reduce
Stray light in fluorescence detection.
The drive electric power unit includes driving power for connecting with fluorescence power supply power end and drives for controlling
The impulse generator of dynamic power supply power supply frequency.
The fluorescent light source includes several excitation lighting sources being uniformly distributed in lampshade, the excitation lighting source packet
It includes as the ultraviolet LED group of excitation light source and for the white LED light source as lighting source；
The ultraviolet LED group include mutually independent first ultraviolet LED excitation light source, the second ultraviolet LED excitation light source with
And third ultraviolet LED excitation light source, the first ultraviolet LED excitation light source emitting ultraviolet light line wavelength be 200nm~285nm, second
Laser LED light source emitting ultraviolet light line wavelength is 365nm~385nm, and third ultraviolet LED excitation light source transmitting ultraviolet wavelength is
405nm。
Light source collector bracket is set on the sample rack, and fluorescent light source and image acquisition device are assemblied in the light
On source collector bracket.
Advantages of the present invention：Drive electric power unit can control the luminous frequency of fluorescent light source, and make the hair of fluorescent light source
The frequency of exposure ratio of light frequency and image acquisition device is positive integer times, to reduce the stray light in fluorescence detection, is improved
Detection efficiency, securely and reliably.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention.
Fig. 2 is the distribution schematic diagram of the excitation lighting source in fluorescent light source of the present invention.
Fig. 3 be ultraviolet LED fluorescence intensity of the present invention, in image acquisition device CCD switch time cyclic graph.
Description of symbols：1- computer, 2- impulse generator, 3- driving power, 4- sample placement rack, 5- fluorescence light
Source, 6- image acquisition device, 7- support connecting plate, 8- light source collector bracket, 9- sample rack, 10- lampshade, 11- white light LEDs light
Source, the first ultraviolet LED of 12- excitation light source, 13- the second ultraviolet LED excitation light source and 14- third ultraviolet LED excitation light source.
Specific embodiment
Below with reference to specific drawings and examples, the invention will be further described.
As shown in Figure 1：In order to effectively reduce the influence of stray light in fluorescence detection, detection efficiency, the present invention are improved
Including for placing the sample placement rack 4 to fluorescence detection sample and being used to support the sample rack of the sample placement rack 4
9, the fluorescent light source 5 of excitation light source and the image acquisition device for acquiring image are also provided as on the sample rack 9
6, the fluorescent light source 5 is located at the surface of sample placement rack 4, and the ultraviolet light that fluorescent light source 5 emits, which can be aligned, to be radiated at
On sample placement rack 4；
It further include the drive electric power unit for driving fluorescent light source 5, the drive electric power unit can control fluorescent light source 5
Luminous frequency, and make fluorescent light source 5 luminous frequency and image acquisition device 6 frequency of exposure ratio be positive integer times, with
Reduce the stray light in fluorescence detection.
Specifically, sample placement rack 4 is normally at the center of sample rack 9, and light source is arranged on the sample rack 9 and adopts
Storage bracket 8, fluorescent light source 5 and image acquisition device 6 are assemblied on the light source collector bracket 8, i.e. light source storage bracket 8
L-shaped, fluorescent light source 5, image acquisition device 6 are mounted on the support connecting plate 7 of 8 upper end of light source collector bracket, light source acquisition
The vertical length direction of device bracket 8 is vertical with sample rack 9.
Fluorescent light source 5 is located at the surface of sample placement rack 4, and the ultraviolet source that fluorescent light source 5 emits, which can compare, to be radiated at
On sample placement rack 4, when placing when the product of fluorescence detection on sample placement rack 4, fluorescent light source 5 can treat fluorescence detection
Product is irradiated excitation fluorescence, using image acquisition device 6 can to the product image to fluorescence detection on sample placement rack 4 into
Row acquisition.
When fluorescent light source 5 works, need to be driven using drive electric power unit, drive electric power unit can control fluorescence light
The luminous frequency in source 5 is positive integer times when making frequency of exposure ratio of the luminous frequency of fluorescent light source 5 with image acquisition device 6,
To reduce the stray light in fluorescence detection.
Further, described image collector 6 includes the colour TV camera with CCD acquisition memory, image acquisition device 6
Also it is electrically connected with the computer 1 with image-capable.
In the embodiment of the present invention, the image of fluorescence reaction can be acquired using image acquisition device 6, computer 1 can adopt image
Storage 6 acquire fluorescent image carry out real-time display, and can pass through the common technological means of the art to fluorescent image into
Row image procossing is specifically known to those skilled in the art says to process of image procossing etc., and details are not described herein again.
Further, the drive electric power unit include driving power 3 for being connect with 5 power end of fluorescence power supply and
For controlling the impulse generator 2 of 3 frequency of supply of driving power.
In the embodiment of the present invention, existing common circuit form is can be used in driving power 3, impulse generator 2, as long as energy
Realize the control to 5 luminous frequency of fluorescent light source, details are not described herein again.In addition, impulse generator 2 can also and computer
1 connection, can control the pulse generation frequency and amplitude of impulse generator 2 by computer 1.It is, of course, also possible to be sent out by pulse
The adjusting knob etc. of raw device 2 itself, is able to achieve and pulse generation frequency, the amplitude of impulse generator 2 is adjusted, specially originally
Known to technical field personnel say, details are not described herein again.
Further, the fluorescent light source 5 includes several excitation lighting sources being uniformly distributed in lampshade 10, described to swash
Sending out lighting source includes as the ultraviolet LED group of excitation light source and for the white LED light source 11 as lighting source；
The ultraviolet LED group includes mutually independent first ultraviolet LED excitation light source 12, the second ultraviolet LED excitation light source
13 and third ultraviolet LED excitation light source 14,12 emitting ultraviolet light line wavelength of the first ultraviolet LED excitation light source be 200nm~
285nm, 13 emitting ultraviolet light line wavelength of second laser LED light source are 365nm~385nm, and third ultraviolet LED excitation light source 14 is sent out
Penetrating ultraviolet wavelength is 405nm.
As shown in Fig. 2, for the present invention specific implementation when fluorescent light source 5 schematic diagram, wherein white LED light source 11 with
Ultraviolet LED group is evenly distributed in lampshade 10 as excitation lighting source, excitation lighting source.When it is implemented, every type
The ultraviolet LED excitation light source power supply process of type is mutually indepedent, can be more to provide the ultraviolet light of different light intensity and different luminous frequencies
Be conducive to use the detection ultraviolet light of different-waveband, light intensity, frequency instead for different test objects.In order to guarantee the uniform of light source
Property, can arrange LED light source according to the arrangement mode of Fig. 2, can also select square, diamond shape etc. arrangement mode, but must protect
Demonstrate,prove the uniformity of light source.In order to keep stable luminous intensity, the ultraviolet LED of wave band of the same race should select same batch products, and light source
Quantity must not be very few, it is proposed that the LED quantity of every kind of wavelength is no less than 3.
When it is implemented, driving power 3 is the power supply of fluorescent light source 5, impulse generator 2 controls driving power 3
Frequency of supply f, to obtain the luminous frequency f of fluorescent light source 5, driving power 3 need to have at least three ports (or with it is ultraviolet
The consistent port number of LED wave band quantity), to guarantee to control the luminous frequency and luminous intensity of fluorescent light source 5 respectively, protect simultaneously
Light source pulse formula is demonstrate,proved to shine.
According to the above description, the fluorescence detection method of ultraviolet LED of the present invention, including for placing to fluorescence detection sample
Sample placement rack 4 and the sample rack 9 for being used to support the sample placement rack 4, are also provided as on the sample rack 9
The fluorescent light source 5 of excitation light source and image acquisition device 6 for acquiring image, the fluorescent light source 5 are located at sample placement rack 4
Surface, the ultraviolet light that fluorescent light source 5 emits can be aligned and is radiated on sample placement rack 4；
It further include the drive electric power unit for driving fluorescent light source 5, the drive electric power unit can control fluorescent light source 5
Luminous frequency, and make fluorescent light source 5 luminous frequency and image acquisition device 6 frequency of exposure ratio be positive integer times, with
Reduce the stray light in fluorescence detection.
In the embodiment of the present invention, organic fluorescent substance mainly shows its fluorescence in ultraviolet spectra and mainly in visible light
It is shown in spectrum, the typical life of fluorescent state is 10-9~10-8Second.According to Beer-Lambert law, fluorescence intensity IFIt is proportional to strong
Degree is I0Incident light and material concentration M product, i.e. IF∝M·I0.Fluorescence intensity I under different timeNWith image acquisition device
CCD frequency of exposure f in 6CCD(t) with the fluorescence radiation frequency f of fluorescent light source 5LED(t) related.As shown in figure 3, the two frequency is not
Matching causes bandwidth and colour temperature is unstable.Further, since fluorescent light source 5 is LED module, Chang Yinwei pulse-modulated signal duty
It is smaller to cause the LED opening time short, and then generate mould group and phenomena such as flashing and brightness irregularities occur.
Therefore, fluorescent light source pulse modulation method mainly includes two aspects in the embodiment of the present invention：
1), the pulse width to be shone using pulse-width modulation method periodically-varied LED, i.e. duty ratio, work as refreshing frequency
When reaching 60Hz or more, human eye can not perceive the shake of each specific LED pixel.It similarly, can by adjusting refreshing frequency
To guarantee that image acquisition device 6 can not perceive the shake of LED pixel.To obtain stable ultraviolet LED luminous intensity, it is necessary to assure
The occupied time t of unblocked level of impulse generator 2 and fluorescent light source 5LEDHave compared with big space rate；Therefore, it needs to pass through arteries and veins first
It rushes generator 2 and obtains the occupied time t of different type LED light source unblocked levelLEDAnd shared by each impulse modulation period
Time tpulse；Then, judge the occupied time t of the LED unblocked levelLEDWhether the impulse modulation period is greater than
Occupied time tpulseHalf, if so, by by tpulseIncrease until tLED≤0.5*tpulse.If it is not, being not necessarily to
Adjust tpulse。
2) it, is also required to the frequency of exposure f of CCD in adjustment image acquisition device 6 simultaneouslyCCD(t) it is sent out with the fluorescence of fluorescent light source 5
Light frequency fLED(t) periodic ratio n.According to different fluorescence detection object modification CCD frequency of exposure fCCD(t) and fluorescent light source
5 fluorescence radiation frequency fLED(t) periodic ratio n,
N=fLED(t)/fCCD(t)
I.e. when n is integer, the CCD exposure image moment is the instantaneous maximum power of fluorescent light source 5 in image acquisition device 6
Moment can reduce the influence of stray light in fluorescence detection, guarantee detection efficiency；In addition, n can also be according to different applications
The functional relation of sin or cos is presented in occasion, specifically can according to need and is selected, details are not described herein again.
Claims (10)
1. a kind of fluorescence detection device of ultraviolet LED, it is characterized in that：Including for placing the sample placement to fluorescence detection sample
Seat (4) and the sample rack (9) for being used to support the sample placement rack (4), are also provided as on the sample rack (9)
The fluorescent light source (5) of excitation light source and image acquisition device (6) for acquiring image, the fluorescent light source (5) are located at sample
The surface of placement rack (4), the ultraviolet light of fluorescent light source (5) transmitting, which can be aligned, to be radiated on sample placement rack (4)；
It further include the drive electric power unit for driving fluorescent light source (5), the drive electric power unit can control fluorescent light source (5)
Luminous frequency, and make fluorescent light source (5) luminous frequency and image acquisition device (6) frequency of exposure ratio be positive integer
Times, to reduce the stray light in fluorescence detection.
2. the fluorescence detection device of ultraviolet LED according to claim 1, it is characterized in that：Described image collector (6) includes
Colour TV camera with CCD acquisition memory, image acquisition device (6) are also electric with the computer (1) with image-capable
Connection.
3. the fluorescence detection device of ultraviolet LED according to claim 1, it is characterized in that：The drive electric power unit includes
Driving power (3) for being connect with fluorescence power supply (5) power end and the pulse for controlling driving power (3) frequency of supply
Generator (2).
4. the fluorescence detection device of ultraviolet LED according to claim 2, it is characterized in that：The drive electric power unit includes
Driving power (3) for being connect with fluorescence power supply (5) power end and the pulse for controlling driving power (3) frequency of supply
Generator (2), the impulse generator (2) connect with computer (1), and computer (1) can control the pulse of impulse generator (2)
Occurrence frequency and amplitude.
5. the fluorescence detection device of ultraviolet LED according to claim 1, it is characterized in that：The fluorescent light source (5) is if include
The dry excitation lighting source being uniformly distributed in lampshade (10), the excitation lighting source includes as the ultraviolet of excitation light source
LED group and for the white LED light source (11) as lighting source；
The ultraviolet LED group includes mutually independent first ultraviolet LED excitation light source (12), the second ultraviolet LED excitation light source
(13) and third ultraviolet LED excitation light source (14), first ultraviolet LED excitation light source (12) emitting ultraviolet light line wavelength are
200nm~285nm, second laser LED light source (13) emitting ultraviolet light line wavelength are 365nm~385nm, and third ultraviolet LED swashs
It is 405nm that light emitting source (14), which emits ultraviolet wavelength,.
6. the fluorescence detection device of ultraviolet LED according to claim 1, it is characterized in that：It is set on the sample rack (9)
Light source collector bracket (8) is set, fluorescent light source (5) and image acquisition device (6) are assemblied in the light source collector bracket (8)
On.
7. a kind of fluorescence detection method of ultraviolet LED, it is characterized in that：Including for placing the sample placement to fluorescence detection sample
Seat (4) and the sample rack (9) for being used to support the sample placement rack (4), are also provided as on the sample rack (9)
The fluorescent light source (5) of excitation light source and image acquisition device (6) for acquiring image, the fluorescent light source (5) are located at sample
The surface of placement rack (4), the ultraviolet light of fluorescent light source (5) transmitting, which can be aligned, to be radiated on sample placement rack (4)；
It further include the drive electric power unit for driving fluorescent light source (5), the drive electric power unit can control fluorescent light source (5)
Luminous frequency, and make fluorescent light source (5) luminous frequency and image acquisition device (6) frequency of exposure ratio be positive integer
Times, to reduce the stray light in fluorescence detection.
8. the fluorescence detection method of ultraviolet LED according to claim 7, it is characterized in that：The drive electric power unit includes using
In the driving power (3) being connect with fluorescence power supply (5) power end and for controlling driving power (3) frequency of supply pulse hair
Raw device (2).
9. the fluorescence detection method of ultraviolet LED according to claim 7, it is characterized in that：The fluorescent light source (5) includes several
The excitation lighting source being uniformly distributed in lampshade (10), the excitation lighting source include the ultraviolet LED as excitation light source
Group and for the white LED light source (11) as lighting source；
The ultraviolet LED group includes mutually independent first ultraviolet LED excitation light source (12), the second ultraviolet LED excitation light source
(13) and third ultraviolet LED excitation light source (14), first ultraviolet LED excitation light source (12) emitting ultraviolet light line wavelength are
200nm~285nm, second laser LED light source (13) emitting ultraviolet light line wavelength are 365nm~385nm, and third ultraviolet LED swashs
It is 405nm that light emitting source (14), which emits ultraviolet wavelength,.
10. the fluorescence detection method of ultraviolet LED according to claim 7, it is characterized in that：It is set on the sample rack (9)
Light source collector bracket (8) is set, fluorescent light source (5) and image acquisition device (6) are assemblied in the light source collector bracket (8)
On.
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