CN108929315B - 3R-indolylmethyl-6S-methionine modified piperazine-2, 5-dione, and synthesis, activity and application thereof - Google Patents
3R-indolylmethyl-6S-methionine modified piperazine-2, 5-dione, and synthesis, activity and application thereof Download PDFInfo
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Abstract
The invention discloses the following formula(3R,6S) -3- (Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione of (1). Discloses a preparation method thereof and discloses the anti-tumor metastasis activity thereof, and thus the invention discloses the application thereof in preparing anti-tumor metastasis medicaments.
Description
Technical Field
The invention relates to (3R,6S) -3- (Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione. Relates to a preparation method thereof and relates to the anti-tumor metastasis activity thereof, so that the invention relates to the application thereof in anti-tumor metastasis medicaments. The invention belongs to the field of biological medicine.
Background
Tumors seriously threaten the health of human beings. In addition to the poor prognosis of patients with tumors by themselves, metastasis associated with tumors further worsens the prognosis of patients. For example, more than 90% of patients with tumors die from metastases. Because the existing antitumor drugs have no effect of resisting tumor metastasis, the clinical curative effect of tumor chemotherapy is not ideal. The invention relates to a medicament for resisting tumor metastasis, which is an urgent clinical need. The inventors have previously disclosed that diketopiperazines of the four S, S-, R, R-, R, S-and S, R-configurations inhibit migration and invasion of HCCLM3 (highly metastatic human liver cancer cells) at a concentration of 0.5. mu.M. Later, the inventors further disclosed that R, R-configured diketopiperazines inhibited tumor metastasis to the lung in C57BL/6 mice at a dose of 5. mu. mol/kg. However, the lowest effective dose is 5. mu. mol/kg. To reduce the minimum effective dose, the inventors have developed various modifications to the amino n-butyl group of the diketopiperazine in the S, R-configuration. After 3 years of exploration, it was found that acylation of the amino n-butyl group of the S, R-diketopiperazine with methionine-acylated amino n-hexanoic acid reduced the minimum effective dose of anti-tumor metastasis to 0.5. mu. mol/kg. Because the toxic and side effects of the medicine can disappear along with the reduction of the dosage, the reduction of the effective dosage by 10 times shows that the structure modification has outstanding technical effect. Based on these findings, the inventors have proposed the present invention.
Disclosure of Invention
In a first aspect of the invention there is provided (3R,6S) -3- (Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione of the formula.
The second aspect of the present invention provides a method for synthesizing (3R,6S) -3- (Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione, which comprises:
(1) D-Boc-Lys (Cbz) and L-Trp-OBzl are condensed to obtain D-Boc-Lys (Cbz) -D-Trp-OBzl;
(2) removing Boc from D-Boc-Lys (Cbz) -D-Trp-OBzl in ethyl acetate solution of hydrogen chloride to obtain D-Lys (Cbz) -D-Trp-OBzl;
(3) cyclizing D-Lys (Cbz) -D-Trp-OBzl in a saturated ethyl acetate solution of a 5% sodium bicarbonate water solution to obtain (3R,6S) -3- (benzyloxycarbonylamino n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (1);
(4) the compound 1 is subjected to hydrogenolysis to remove benzyloxycarbonyl to obtain (3R,6S) -3- (amino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-diketone (2);
(5) condensing amino methyl hexanoate and Boc-Met to obtain Boc-Met-amino methyl hexanoate (3);
(6) saponifying the compound 3 to remove methyl ester to obtain Boc-Met-amino-n-hexanoic acid (4);
(7) condensing the compound 2 and the compound 4 to obtain (3R,6S) -3- (Boc-Met-amino-n-hexanoyl amino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-diketone (5).
(8) The compound 5 is subjected to Boc removal in an ethyl acetate solution of hydrogen chloride to obtain (3R,6S) -3- (Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (6).
The third aspect of the present invention is to evaluate the activity of (3R,6S) -3- (Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione in inhibiting the metastasis of lung cancer in C57BL/6 mice.
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FIG. 1(3R,6S) -3- (Met-amino-N-hexanoylamino-N-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (6) synthetic route I) Dicyclohexylcarbodiimide (DCC), 1-hydroxybenzotriazole (HOBt), N-methylmorpholine (NMM); ii) a solution of hydrogen chloride in ethyl acetate; iii) ethyl acetate, 5% aqueous sodium bicarbonate; iv) Pd/C, H2(ii) a v) methanol, NaOH (2M).
Detailed Description
To further illustrate the invention, a series of examples are given below. These examples are purely illustrative and are intended to be a detailed description of the invention only and should not be taken as limiting the invention.
EXAMPLE 1 preparation of D-Boc-Lys (Cbz) -L-Trp-OBzl
To a solution of 1.90g (5.0mmol) of D-Boc-Lys (Cbz) and 20mL of dried Tetrahydrofuran (THF) were added 0.68g (5.0mmol) of 1-hydroxybenzotriazole (HOBt) and 1.24g (6.0mmol) of Dicyclohexylcarbodiimide (DCC) under ice-cooling and stirred for 30 minutes to obtain reaction solution A. 1.47g (5.0mmol) of L-Trp-OBzl was dissolved in 20mL of dry THF, and N-methylmorpholine (NMM) was added to adjust the pH to 9 to obtain reaction solution B. The reaction solution B was added to the reaction solution A and reacted at room temperature for 12 hours, and TLC (methylene chloride/methanol, 40/1) showed completion of the reaction. The reaction mixture was filtered, the filtrate was concentrated under reduced pressure, and the residue was dissolved in 50mL of ethyl acetate. The resulting solution was sequentially saturated NaHCO3Aqueous solution (25 mL. times.3), saturated aqueous NaCl solution (25 mL. times.3), 5% KHSO4Aqueous wash (25 mL. times.3), saturated aqueous NaCl wash (25 mL. times.3), saturated aqueous NaHCO3An aqueous wash (25 mL. times.3) and a saturated aqueous NaCl wash (25 mL. times.3). Anhydrous Na for ethyl acetate layer2SO4Drying for 12 hours. Filtering off Na2SO4The filtrate was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (dichloromethane/methanol, 40/1) to give 2.94g (90%) of the title compound as a colorless solid. ESI-MS (M/z):657[ M + H]+。
EXAMPLE 2 preparation of D-Lys (Cbz) -L-Trp-OBzl
To 2.62g (4.0mmol) of D-Boc-Lys (Cbz) -L-Trp-OBzl was slowly added 30mL of a solution of hydrogen chloride in ethyl acetate (4M) under ice-bath and stirred for 4 hours, TLC (dichloromethane/methanol, 40/1) showed the reaction was complete. The reaction mixture was concentrated under reduced pressure, and the residue was dissolved in 30mL of anhydrous ethyl acetate, and the resulting solution was concentrated under reduced pressure, and the residue was dissolved in 30mL of anhydrous ethyl acetate. This operation was repeated three times. The residue was suspended with 30mL of anhydrous ether by means of a sonicator and after standing the ether was removed to give 2.07g (93%) of the title compound as a yellow powder. ESI-MS (M/z):557[ M + H ]]+。
EXAMPLE 3 preparation of (3R,6S) -3- (benzyloxycarbonylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (1)
A solution of 1.95g (3.5mmol) D-Lys (Cbz) -L-Trp-OBzl and 50mL ethyl acetate was treated with saturated NaHCO3After the aqueous solution was sufficiently washed (25 mL. times.3), the ethyl acetate layer was stirred at 80 ℃ for 56 hours. TLC (dichloromethane/methanol, 20/1) showed the reaction was complete. The reaction mixture was allowed to stand well at room temperature and filtered to give 0.72g (46%) of the title compound as a colorless solid. ESI-MS (M/z):449[ M + H ]]+。
EXAMPLE 4 preparation of (3R,6S) -3- (amino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (2)
To a solution of 0.67g (1.5mmol) of (3R,6S) -3- (benzyloxycarbonylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (1) in 10mL of Dimethylformamide (DMF) was added 0.07g of Pd/C (10%) and the mixture was taken up in H at room temperature2TLC (dichloromethane/methanol, 20/1) showed the reaction was complete for 48 hours. Pd/C was filtered off from the reaction mixture, and the filtrate was concentrated under reduced pressure to give 0.48g (95%) of the title compound as a colorless solid. ESI-MS (M/z) 315[ M + H ]]+。
EXAMPLE 5 preparation of amino-n-hexanoic acid methyl ester
5.2mL of thionyl chloride was added dropwise to 52mL of methanol under ice-cooling, stirred for 30 minutes, and 0.26g (2.0mmol) of amino-n-hexanoic acid was added to the pellets. The reaction mixture was stirred at room temperature for 24 hours. TLC (dichloromethane/methanol, 3/1) showed the reaction was complete. The reaction mixture was concentrated under reduced pressure, the residue was dissolved in 30mL of methanol, and the resulting solution was concentrated under reduced pressure. This operation was repeated three times. Addition of the residue30mL of anhydrous ether were thoroughly suspended by means of a sonicator and the ether was removed to give 0.27g (94%) of the title compound as a colorless powder. ESI-MS (M/e) 145[ M + H]+。
EXAMPLE 6 preparation of Boc-Met-amino-n-hexanoic acid methyl ester (3)
From 0.37g (1.5mmol) Boc-Met and 0.22g (1.5mmol) methyl aminohexanoate, 0.51g (90%) of the title compound was obtained as colorless solid by the method of example 1. ESI-MS (M/z):411[ M + H]+。
EXAMPLE 7 preparation of Boc-Met-amino-n-hexanoic acid (4)
0.45g (1.2mmol) Boc-Met-amino-n-hexanoic acid methyl ester (3) was dissolved in 8mL methanol, the pH was adjusted to 12 with aqueous NaOH (2M) in ice bath, stirred at room temperature for 4 h, and TLC (dichloromethane/methanol, 20/1) showed completion of the reaction. The reaction mixture was saturated with KHSO in ice bath4Adjusting pH of the aqueous solution to 7, concentrating under reduced pressure, and adding saturated KHSO to the aqueous phase4The aqueous solution was adjusted to pH 2 and extracted thoroughly three times with 10mL of ethyl acetate. The ethyl acetate layer was washed three times with 10mL of a saturated aqueous NaCl solution to give a solution pH of 7, and then with anhydrous Na2SO4Drying for 12 hours. Filtering to remove Na2SO4The filtrate was concentrated under reduced pressure to give 0.41g (94%) of the title compound as a colorless solid. ESI-MS (M/z):397[ M + H]+。
EXAMPLE 8 preparation of (3R,6S) -3- (Boc-Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (5)
To a solution of 0.36g (1.0mmol) of Boc-Met-amino-n-hexanoic acid (4) and 5mL of anhydrous DMF was added 0.14g (1.0mmol) of HOBt and 0.25g (1.2mmol) of DCC under ice-bath, and stirred for 30 minutes to obtain reaction solution A. 0.31g (1.0mmol) of (3R,6S) -3- (amino-N-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (2) was dissolved in 5mL of anhydrous DMF, and pH was adjusted to 9 with N-methylmorpholine to give reaction solution B. Reaction B was added to reaction A and stirred at room temperature for 12 hours, and TLC (dichloromethane/methanol, 10/1) showed completion of the reaction. The reaction mixture was filtered, the filtrate was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (dichloromethane/methanol, 50/1) to give 0.12g (18%) of the title compound as a colorless solid. ESI-MS (M/z):693[ M + H]+;1H NMR(300MHz,DMSO-d6)δ/ppm=10.887(s,1H),8.031(d,J=2.1Hz,1H),7.884(s,1H),7.755(t,J=5.4Hz,1H),7.674(t,J=5.4Hz,1H),7.569(d,J=7.8Hz,1H),7.317(d,J=7.8Hz,1H),7.045(m,2H),6.947(td,J1=8.1Hz,J2=0.9Hz,1H),4.071(m,1H),4.000(m,1H),3.287(dd,J1=14.4Hz,J2=4.2Hz,1H),3.008(m,6H),2.412(m,2H),2.028(s,3H),1.994(m,4H),1.777(m,2H),1.377(m,15H),1.181(m,6H)。
EXAMPLE 9 preparation of (3R,6S) -3- (Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (6)
From 0.07g (0.1mmol) of (3R,6S) -3- (Boc-Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (5) according to the method of example 2, 0.05g (91%) of the title compound are obtained as colorless solid. ESI-MS (M/z):559[ M + H]+;Mp 146-148℃;(c ═ 0.1, methanol); IR (cm)-1):3223,3078,2924,2851,1654,1543,1443,1326,1237,1096,740;1H NMR(300MHz,DMSO-d6)δ/ppm=10.922(s,1H),8.500(t,J=5.4Hz,1H),8.234(s,2H),8.042(d,J=1.9Hz,1H),7.847(s,1H),7.720(t,J=5.4Hz,1H),7.568(d,J=7.8Hz,1H),7.320(d,J=7.8Hz,1H),7.043(m,2H),6.947(td,J1=8.1Hz,J2=0.9Hz,1H),4.073(m,1H),3.791(m,1H),3.255(dd,J1=14.4Hz,J2=4.2Hz,1H),3.059(m,6H),2.481(m,2H),2.058(s,3H),1.994(m,4H),1.438(m,6H),1.229(m,6H)。
EXAMPLE 10 preparation of Boc-amino-n-hexanoic acid
To a solution of 0.26g (2.0mmol) of aminon-hexanoic acid in 5mL of distilled water was added 0.58g (2.6mmol) (Boc) with stirring2Solution of O with 5mL dioxane. The resulting solution was adjusted to pH 9 with an aqueous solution of NaOH (2M) in ice bath. After stirring for 30 minutes in an ice bath, the mixture was stirred at room temperature and pumped down by a water pump. The pH was monitored during stirring and kept at 9 all the time until TLC (dichloromethane/methanol, 3/1) indicated completion of the reaction. The reaction mixture was saturated with KHSO under ice-bath4Adjusting pH of the aqueous solution to 7, and concentrating under reduced pressure. The aqueous phase is saturated KHSO4Adjusting pH to 2 with water solution, washing with 10mL ethyl acetate for three times, washing with 10mL saturated NaCl water solution for three times to make solution pH 7, and adding anhydrous Na2SO4Drying for 12 hours. Filtering to remove Na2SO4The filtrate was concentrated under reduced pressure to give 0.41g (89%) of the title compound as a colorless solid. ESI-MS (M/e):232[ M + H]+。
EXAMPLE 11 preparation of (3R,6S) -3- (Boc-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (7)
From 0.28g (1.2mmol) Boc-amino-n-hexanoic acid and 0.38g (1.2mmol) (3R,6S) -3- (amino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (2) 0.12g (19%) of the title compound was obtained as a colorless solid using the method of example 8. ESI-MS (M/z):528[ M + H]+;1H NMR(300MHz,DMSO-d6)δ/ppm=10.903(s,1H),8.046(d,J=1.8Hz,1H),7.851(s,1H),7.686(t,J=5.4Hz,1H),7.556(d,J=7.8Hz,1H),7.314(d,J=7.8Hz,1H),7.039(m,2H),6.943(td,J1=7.8Hz,J2=0.6Hz,1H),6.758(t,J=5.4Hz,1H),4.068(m,1H),3.252(dd,J1=14.4Hz,J2=4.2Hz,1H),2.924(m,6H),1.995(t,J=7.2Hz,2H),1.292(m,21H)。
EXAMPLE 12 preparation of (3R,6S) -3- (amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (8)
From 0.11g (0.2mmol) (3R,6S) -3- (Boc-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (7) 0.08g (96%) of the title compound was obtained as colorless solid by the method of example 2. ESI-MS (M/z):428[ M + H]+;1H NMR(300MHz,DMSO-d6)δ/ppm=10.924(s,1H),8.071(s,1H),7.866(s,1H),7.729(t,J=5.4Hz,1H),7.568(d,J=7.5Hz,1H),7.321(d,J=7.5Hz,1H),7.044(m,2H),6.949(t,J=7.5Hz,1H),4.070(m,1H),3.239(dd,J1=14.4Hz,J2=3.6Hz,1H),2.991(m,4H),2.694(m,2H),2.021(t,J=7.5Hz,2H),1.467(m,6H),1.240(m,6H)。
EXAMPLE 13 determination of the anti-metastatic Activity of Compound 6
The assay model was inoculated with Lewis mouse lung carcinoma cells (LLC, purchased from ATCC) in DMEM medium10% inactivated fetal bovine serum, 1X 105U/L penicillin and 100mg/L streptomycin), and the cells are enriched by passage every two days according to an adherent cell culture method. Digesting the cells when the cells are in good growth state and in logarithmic growth phase, and adjusting the cell density to 1 × 10 with physiological saline7one/mL. Staining with placental blue to count viable cells>95 percent. Inbred C57BL/6 male mice (SPF grade, body weight 20. + -.2 g) were taken and left-handed mice fixed. The right anterior limb axillary skin of the mouse was disinfected with 75% ethanol. The LLC tumor cell suspension is injected subcutaneously into the axilla of a mouse with a 1mL sterile syringe held in the right hand, and 0.2mL is injected into each mouse. After the mice are inoculated for 10 days, tumors with the diameter of about 4-5mm grow out, namely the tumor source. The Lewis lung cancer tumor-bearing mice are inoculated for 10 days and anesthetized by ether, and then the cervical vertebrae are removed for killing. Soaking in 75% ethanol for 10min, sterilizing, and removing tumor on clean bench. Well-grown tumor tissue was selected, minced in a sterile plate, and placed in a tissue homogenizer made of glass. Adding physiological saline with the temperature of 4 ℃ according to the ratio of the tumor mass to the volume of the physiological saline of 1 to 3(g to mL), and lightly grinding to prepare the cell suspension. The cell suspension is screened by 200-mesh cells to prepare single cell suspension. Adjusting the cell density of the single cell suspension to 1.5X 10 with physiological saline7one/mL. Staining with placental blue to count viable cells>95 percent. Left-handed inbred C57BL/6 male mice were fixed and their right anterior limb axillary skin was disinfected with 75% ethanol. The tumor cell suspension was injected subcutaneously into the mouse axilla with a 1mL sterile syringe in the right hand, 0.2mL each. 10 days after inoculation, the mice developed tumors of 4-5mm in diameter, and the inoculated mice were randomly grouped by the measured tumor volume. Each group had 12 mice. Mice on day 11 of tumor inoculation were orally administered 1 dose per day for 12 consecutive days with either a 20 μmol/kg/day (dose) normal saline solution of the putative anti-tumor metastasis peptide Arg-Gly-Asp-Ser (RGDS) or compound 8 (dose 5 μmol/kg/day) or compound 6 (dose 0.5 μmol/kg/day) or 10 mL/kg/day. The next day of the last administration, cervical spine was removed by ether anesthesia, and the lungs of the mice were taken and the number of tumor nodules that metastasized in the tumor lungs was calculated. Statistical analysis of the data was performed using the t-test. The results are shown in Table 1. At a dose of 0.5. mu. mol/kg Compound 6 is not onlyEffectively inhibit tumor lung metastasis, and the activity is not significantly different from RGDS which is 40 times higher in dosage and compound 8 which is 10 times higher in dosage. These data indicate that the present invention has significant technical effects.
TABLE 1 antitumor metastatic Activity of Compound 6
a) P <0.01 to saline, p >0.05 to RGDS and compound 8; n is 12.
Claims (3)
2. a process for the preparation of (3R,6S) -3- (Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione as claimed in claim 1, which comprises:
(1) D-Boc-Lys (Cbz) and L-Trp-OBzl are condensed to obtain D-Boc-Lys (Cbz) -L-Trp-OBzl;
(2) removing Boc from D-Boc-Lys (Cbz) -L-Trp-OBzl in ethyl acetate solution of hydrogen chloride to obtain D-Lys (Cbz) -L-Trp-OBzl;
(3) washing the ethyl acetate solution of D-Lys (Cbz) -L-Trp-OBzl with saturated sodium bicarbonate water solution and cyclizing to obtain (3R,6S) -3- (benzyloxycarbonylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (1);
(4) the compound 1 is subjected to hydrogenolysis to remove benzyloxycarbonyl to obtain (3R,6S) -3- (amino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-diketone (2);
(5) condensing amino methyl hexanoate and Boc-Met to obtain Boc-Met-amino methyl hexanoate (3);
(6) saponifying the compound 3 to remove methyl ester to obtain Boc-Met-amino-n-hexanoic acid (4);
(7) condensing the compound 2 and the compound 4 to obtain (3R,6S) -3- (Boc-Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-diketone (5);
(8) the compound 5 is subjected to Boc removal in an ethyl acetate solution of hydrogen chloride to obtain (3R,6S) -3- (Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione (6).
3. The use of (3R,6S) -3- (Met-amino-n-hexanoylamino-n-butyl) -6- (indole-3-methyl) -piperazine-2, 5-dione according to claim 1 for the preparation of a medicament for the treatment of tumor metastasis.
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CN108929315A (en) | 2018-12-04 |
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