CN108927528A - A method of nanometer platinum particles are prepared by template of Auricularia polysaccharide - Google Patents
A method of nanometer platinum particles are prepared by template of Auricularia polysaccharide Download PDFInfo
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- CN108927528A CN108927528A CN201810729495.XA CN201810729495A CN108927528A CN 108927528 A CN108927528 A CN 108927528A CN 201810729495 A CN201810729495 A CN 201810729495A CN 108927528 A CN108927528 A CN 108927528A
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- auricularia polysaccharide
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/05—Metallic powder characterised by the size or surface area of the particles
- B22F1/054—Nanosized particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/06—Metallic powder characterised by the shape of the particles
- B22F1/065—Spherical particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/07—Metallic powder characterised by particles having a nanoscale microstructure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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Abstract
A method of nanometer platinum particles are prepared by template of Auricularia polysaccharide, its ratio for being mainly 1:1 in distilled water and Auricularia polysaccharide mass ratio, compound concentration is the Auricularia polysaccharide solution of 1mg/mL, it is uniformly mixed in the ratio that the molar ratio of Auricularia polysaccharide solution and Tetrachloroplatinate potassium solution is 1:300~600, it puts it into metal bath, 300~500rpm, reacts 3h at 40 DEG C~55 DEG C, solution slowly becomes Dark grey from colourless, to obtain a nanometer platinum particles.The present invention has many advantages, such as that reaction condition is mild, easy to operate, raw material is environmentally friendly and dispersion stabilization is good.
Description
Technical field
The invention belongs to technical field of metal material, in particular to a kind of preparation method of nano material.
Background technique
Enzyme is a kind of natural biocatalyst, has good catalytic efficiency and selectivity.However, native enzyme have at
The disadvantages of this height, easy in inactivation, which has limited its applications in many aspects.Noble metal nano particles are as a kind of important nanometer
Material is capable of the catalytic activity of Mimetic enzyme, is widely used in various detections.Result of study shows that noble metal is received
The partial size of rice corpuscles is smaller, and specific surface area is bigger, and active site is more, and catalytic activity is bigger.Therefore, your small gold of partial size prepared
Belong to nanoparticle with the catalytic activity of Mimetic enzyme, has a good application prospect.
Currently, template is a kind of method of very important preparation nanometer platinum particles.The characteristics of template is either
The chemical reaction occurred in liquid phase or gas phase can carry out in the effective coverage of template, therefore, can regulate and control a nanometer platinum grain
The partial size and pattern of son, prevent Platinum Nanoparticles particle aggregation.According to the difference of limit threshold energy power, template is divided into hard template method and soft mode
Plate method.Hard template method has structure more single, and prepared nanoparticle pattern changes less disadvantage；Soft template method has
The simple advantage of operating method, it is template, common lysozyme, trypsase and ox that soft template method, which generallys use polymer substance,
Though the protein molecules such as seralbumin can prepare mono-dispersed nano platinum particles, the method has cost of material high
Disadvantage, and the macromolecules such as polyethyleneimine can not natural degradation, and it is toxic.Therefore, it is also desirable to further develop newly
Prepare the soft template of nanometer platinum particles.
Summary of the invention
Mild, operation that the purpose of the present invention is to provide a kind of preparation conditions is easy, raw material environmental protection is with Auricularia polysaccharide
The method of template preparation nanometer platinum particles.The present invention mainly prepares Auricularia polysaccharide solution first, utilizes the reduction of Auricularia polysaccharide
Property reducing metal platinum presoma, obtains the nanometer platinum particles of uniform particle sizes.
Technical scheme is as follows:
(1) ratio for being 1:1 in the mass ratio of distilled water and Auricularia polysaccharide is configured to the Auricularia polysaccharide that concentration is 1mg/mL
Solution；
(2) it is uniformly mixed in the ratio that the molar ratio of Auricularia polysaccharide solution and Tetrachloroplatinate potassium solution is 1:300~600,
It putting it into metal bath, 300~500rpm, 3h is reacted at 40~55 DEG C, solution slowly becomes Dark grey from colourless, thus
To nanometer platinum particles.
The invention has the following advantages over the prior art:
1, preparation process is simple, low in cost, and reaction condition is mild.
2, the morphological rules of obtained nano platinum particle, uniform particle sizes, stability are good.
3, selected template itself has very high biocompatibility, and product can be used for field of biomedicine.
Detailed description of the invention
Fig. 1 is Auricularia polysaccharide-nano platinum particle TEM figure that the embodiment of the present invention 1 obtains.
Fig. 2 is 1 Mimetic enzyme optimum temperature figure of present example.
Fig. 3 is 1 Mimetic enzyme Optimal pH figure of present example.
Fig. 4 is that present example 1 is applied to linear graph between the glucose absorbance and concentration of detection various concentration.
Fig. 5 is Auricularia polysaccharide-nano platinum particle TEM figure that the embodiment of the present invention 2 obtains.
Fig. 6 is Auricularia polysaccharide-nano platinum particle TEM figure that the embodiment of the present invention 3 obtains.
Specific embodiment
Embodiment 1
It takes Auricularia polysaccharide to be dissolved in distilled water, is configured to the Auricularia polysaccharide solution that concentration is 1mg/mL, Auricularia polysaccharide is molten
Liquid and Tetrachloroplatinate potassium solution are put into metal bath after mixing in the ratio of 1:300, and control revolving speed is 500rpm, and temperature is 40 DEG C
Under the conditions of react 3h, solution slowly becomes Dark grey from colourless, i.e., acquisition Auricularia polysaccharide-nanometer platinum particles.
Morphology characterization is carried out to nanometer platinum grain word using transmission electron microscope, as shown in Figure 1, the diameter of nanometer platinum particles
For 0.99nm or so, morphological rules, in spherical.
For the catalytic activity for studying Platinum Nanoparticles particle simulation peroxidase, following experiment is set:
1) the 1000 μ L of buffer solution of identical pH is added in 1 sample of Example, 50 μ L；It is protected at different temperatures with metal bath
300 μ L of TMB is added in warm 10min thereto, keeps the temperature 10min；4) H is added2O250 μ L (0.03M) react under set temperature
10min；Later in ultraviolet lower measurement absorbance, the absorbance value at 652nm wavelength is observed, obtains absorbance with the variation of T
Curve, as shown in Figure 2.
2) the 1000 μ L of buffer solution of different pH is added in 1 sample of Example, 50 μ L；It is protected at the same temperature with metal bath
300 μ L of TMB is added in warm 10min thereto, keeps the temperature 10min；4) H is added2O250 μ L (0.03M) react under set temperature
10min；Later in ultraviolet lower measurement absorbance, the absorbance value at 652nm wavelength is observed, obtains absorbance with the variation of pH
Curve, as shown in Figure 3.
The optimum condition of Platinum Nanoparticles particle simulation peroxidase is obtained by the experiment.
It can be applied to detection concentration of glucose for research Platinum Nanoparticles particle simulation peroxidase, following experiment is set:
Take 100 μ L of glucose oxidase (GOx), be separately added into the 200 μ L of glucose of various concentration (0.04mM-1mM) in
2mL centrifuge tube heats 30min at 50 DEG C of metal bath；PH=4 is added in another centrifuge tube in 1 sample of another Example, 200 μ L
300 μ L of Acetic acid-sodium acetate buffer, add 700 μ L TMB solution, heat 15min at 25 DEG C of metal bath optimum temperature；It will
Above-mentioned TMB solution is added separately in each glucose solution, reacts 30min at 25 DEG C of metal bath；
Absorbance is measured at 652nm using ultraviolet, as shown in figure 4, when concentration of glucose is between 0.04-1mM, extinction
It is between degree and concentration good linear relationship (y=0.45803x+0.05257), therefore illustrates to simulate peroxide using the nanoparticle
The method sensitivity that compound enzyme detects glucose is higher.
Embodiment 2
It takes Auricularia polysaccharide to be dissolved in distilled water, is configured to the Auricularia polysaccharide solution that concentration is 1mg/mL, Auricularia polysaccharide is molten
Liquid and Tetrachloroplatinate potassium solution are put into metal bath after mixing in the ratio of 1:500, and control revolving speed is 400rpm, and temperature is 50 DEG C
Under the conditions of react 3h, solution slowly becomes Dark grey from colourless, i.e., acquisition Auricularia polysaccharide-nanometer platinum particles.
Morphology characterization is carried out to nanometer platinum particles using transmission electron microscope, as shown in figure 5, the diameter of nanometer platinum particles
For 1.19nm or so, morphological rules, in spherical.
Embodiment 3
It takes Auricularia polysaccharide to be dissolved in distilled water, is configured to the Auricularia polysaccharide solution that concentration is 1mg/mL, Auricularia polysaccharide is molten
Liquid and Tetrachloroplatinate potassium solution are put into metal bath after mixing in the ratio of 1:600, and control revolving speed is 300rpm, and temperature is 55 DEG C
Under the conditions of react 3h, solution slowly becomes Dark grey from colourless, i.e., acquisition Auricularia polysaccharide-nanometer platinum particles.
Morphology characterization is carried out to nanometer platinum particles using transmission electron microscope, as shown in fig. 6, the diameter of nanometer platinum particles
For 1.58nm or so, morphological rules, in spherical.
Claims (1)
1. a kind of method for preparing nanometer platinum particles as template using Auricularia polysaccharide, it is characterised in that:
(1) ratio for being 1:1 in distilled water and Auricularia polysaccharide mass ratio, compound concentration are the Auricularia polysaccharide solution of 1mg/mL；
It (2) is the ratio of 1:300~600 in the molar ratio of Auricularia polysaccharide and Tetrachloroplatinate potassium solution, the agaric of step (1) is more
Sugar juice and Tetrachloroplatinate potassium solution are uniformly mixed, and are put into metal bath, 300~500rpm, 3h, solution are reacted at 40~55 DEG C
Slowly become Dark grey from colourless, to obtain a nanometer platinum particles.
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| CN201810729495.XA CN108927528A (en) | 2018-07-05 | 2018-07-05 | A method of nanometer platinum particles are prepared by template of Auricularia polysaccharide |
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| CN201810729495.XA CN108927528A (en) | 2018-07-05 | 2018-07-05 | A method of nanometer platinum particles are prepared by template of Auricularia polysaccharide |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009044146A1 (en) * | 2007-10-02 | 2009-04-09 | University Of York | Metal nanoparticles |
| WO2010010123A1 (en) * | 2008-07-23 | 2010-01-28 | Universita' Degli Studi Di Trieste | Three-dimensional nanocomposite materials consisting of a polysaccharidic matrix and metallic nanoparticles, preparation and use thereof |
| CN101733411A (en) * | 2009-12-15 | 2010-06-16 | 武汉大学 | Preparation method of biocompatible Pt nanoparticle |
| CN107486562A (en) * | 2017-11-09 | 2017-12-19 | 西北师范大学 | Plant-derived polyoses extract reducing metal salt prepares method and the application of metal nano material |
-
2018
- 2018-07-05 CN CN201810729495.XA patent/CN108927528A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009044146A1 (en) * | 2007-10-02 | 2009-04-09 | University Of York | Metal nanoparticles |
| WO2010010123A1 (en) * | 2008-07-23 | 2010-01-28 | Universita' Degli Studi Di Trieste | Three-dimensional nanocomposite materials consisting of a polysaccharidic matrix and metallic nanoparticles, preparation and use thereof |
| CN101733411A (en) * | 2009-12-15 | 2010-06-16 | 武汉大学 | Preparation method of biocompatible Pt nanoparticle |
| CN107486562A (en) * | 2017-11-09 | 2017-12-19 | 西北师范大学 | Plant-derived polyoses extract reducing metal salt prepares method and the application of metal nano material |
Non-Patent Citations (2)
| Title |
|---|
| 邓兰青等: "多糖模板调控纳米无机晶体生长", 《人工晶体学报》 * |
| 郑晓凤等: "桔梗多糖软模板法制备纳米硒的抗氧化及抗肿瘤活性研究", 《微量元素与健康研究》 * |
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