CN108926506A - The extracting method of flavonols component and its extract and application in roundpod jute leaf - Google Patents
The extracting method of flavonols component and its extract and application in roundpod jute leaf Download PDFInfo
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- CN108926506A CN108926506A CN201811129711.3A CN201811129711A CN108926506A CN 108926506 A CN108926506 A CN 108926506A CN 201811129711 A CN201811129711 A CN 201811129711A CN 108926506 A CN108926506 A CN 108926506A
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- roundpod jute
- jute leaf
- extract
- extracting method
- roundpod
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Links
- 235000011777 Corchorus aestuans Nutrition 0.000 title claims abstract description 82
- 235000010862 Corchorus capsularis Nutrition 0.000 title claims abstract description 82
- 240000000491 Corchorus aestuans Species 0.000 title claims abstract description 81
- 239000000284 extract Substances 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 47
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 150000002216 flavonol derivatives Chemical class 0.000 title claims abstract description 28
- 235000011957 flavonols Nutrition 0.000 title claims abstract description 28
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 claims abstract description 48
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 claims abstract description 47
- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 claims abstract description 46
- 238000000605 extraction Methods 0.000 claims abstract description 43
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 38
- OVSQVDMCBVZWGM-LQSBFMDOSA-N 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3-[(2r,3s,4r,5r,6s)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OVSQVDMCBVZWGM-LQSBFMDOSA-N 0.000 claims abstract description 26
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 claims abstract description 24
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 claims abstract description 24
- 235000005875 quercetin Nutrition 0.000 claims abstract description 24
- 229960001285 quercetin Drugs 0.000 claims abstract description 24
- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 claims abstract description 23
- 235000008777 kaempferol Nutrition 0.000 claims abstract description 23
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 claims abstract description 23
- 150000003254 radicals Chemical class 0.000 claims abstract description 17
- 238000010992 reflux Methods 0.000 claims abstract description 17
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 42
- 239000000243 solution Substances 0.000 claims description 33
- 235000019441 ethanol Nutrition 0.000 claims description 28
- 239000007864 aqueous solution Substances 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 230000001376 precipitating effect Effects 0.000 claims description 10
- -1 DPPH free radical Chemical class 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- 239000001301 oxygen Substances 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 4
- 239000002537 cosmetic Substances 0.000 claims description 4
- 235000013402 health food Nutrition 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 24
- 102000003425 Tyrosinase Human genes 0.000 abstract description 17
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- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 6
- 230000002000 scavenging effect Effects 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 230000007760 free radical scavenging Effects 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
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- 229960004889 salicylic acid Drugs 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 244000062250 Kaempferia rotunda Species 0.000 description 2
- 235000013422 Kaempferia rotunda Nutrition 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- LUJAXSNNYBCFEE-UHFFFAOYSA-N Quercetin 3,7-dimethyl ether Natural products C=1C(OC)=CC(O)=C(C(C=2OC)=O)C=1OC=2C1=CC=C(O)C(O)=C1 LUJAXSNNYBCFEE-UHFFFAOYSA-N 0.000 description 2
- PUTDIROJWHRSJW-UHFFFAOYSA-N Quercitrin Natural products CC1OC(Oc2cc(cc(O)c2O)C3=CC(=O)c4c(O)cc(O)cc4O3)C(O)C(O)C1O PUTDIROJWHRSJW-UHFFFAOYSA-N 0.000 description 2
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 2
- OXGUCUVFOIWWQJ-XIMSSLRFSA-N acanthophorin B Natural products O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OXGUCUVFOIWWQJ-XIMSSLRFSA-N 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- OEKUVLQNKPXSOY-UHFFFAOYSA-N quercetin 3-O-beta-D-glucopyranosyl(1->3)-alpha-L-rhamnopyranosyl(1->6)-beta-d-galactopyranoside Natural products OC1C(O)C(C(O)C)OC1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OEKUVLQNKPXSOY-UHFFFAOYSA-N 0.000 description 2
- QPHXPNUXTNHJOF-UHFFFAOYSA-N quercetin-7-O-beta-L-rhamnopyranoside Natural products OC1C(O)C(O)C(C)OC1OC1=CC(O)=C2C(=O)C(O)=C(C=3C=C(O)C(O)=CC=3)OC2=C1 QPHXPNUXTNHJOF-UHFFFAOYSA-N 0.000 description 2
- OXGUCUVFOIWWQJ-HQBVPOQASA-N quercitrin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OXGUCUVFOIWWQJ-HQBVPOQASA-N 0.000 description 2
- 238000000825 ultraviolet detection Methods 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 1
- OTEKOJQFKOIXMU-UHFFFAOYSA-N 1,4-bis(trichloromethyl)benzene Chemical compound ClC(Cl)(Cl)C1=CC=C(C(Cl)(Cl)Cl)C=C1 OTEKOJQFKOIXMU-UHFFFAOYSA-N 0.000 description 1
- 235000012871 Arctostaphylos uva ursi Nutrition 0.000 description 1
- 244000139693 Arctostaphylos uva ursi Species 0.000 description 1
- 240000004792 Corchorus capsularis Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- OVSQVDMCBVZWGM-IDRAQACASA-N Hirsutrin Natural products O([C@H]1[C@H](O)[C@H](O)[C@H](O)[C@@H](CO)O1)C1=C(c2cc(O)c(O)cc2)Oc2c(c(O)cc(O)c2)C1=O OVSQVDMCBVZWGM-IDRAQACASA-N 0.000 description 1
- FVQOMEDMFUMIMO-UHFFFAOYSA-N Hyperosid Natural products OC1C(O)C(O)C(CO)OC1OC1C(=O)C2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 FVQOMEDMFUMIMO-UHFFFAOYSA-N 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 229940042795 hydrazides for tuberculosis treatment Drugs 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- GXMWXESSGGEWEM-UHFFFAOYSA-N isoquercitrin Natural products OCC(O)C1OC(OC2C(Oc3cc(O)cc(O)c3C2=O)c4ccc(O)c(O)c4)C(O)C1O GXMWXESSGGEWEM-UHFFFAOYSA-N 0.000 description 1
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- 238000002156 mixing Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- QEVHRUUCFGRFIF-MDEJGZGSSA-N reserpine Chemical compound O([C@H]1[C@@H]([C@H]([C@H]2C[C@@H]3C4=C(C5=CC=C(OC)C=C5N4)CCN3C[C@H]2C1)C(=O)OC)OC)C(=O)C1=CC(OC)=C(OC)C(OC)=C1 QEVHRUUCFGRFIF-MDEJGZGSSA-N 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 229960004441 tyrosine Drugs 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to crop extractive technique fields, extracting method and its extract and application more particularly, to flavonols component in a kind of roundpod jute leaf.The extracting method of flavonols component in the roundpod jute leaf includes the following steps: to carry out refluxing extraction to roundpod jute leaf using the solution of alcohol-containing as extracting solution.Extracting method of the present invention, can isoquercitin, Quercetin and Kaempferol of the depth extraction into roundpod jute leaf, high income, simple process, mild condition；Also, the extract extracted using extracting method of the present invention is acted synergistically by the isoquercitin of specific quantity, Quercetin and Kaempferol three, ability and tyrosinase activity inhibiting effect with excellent removing free radical.
Description
Technical field
The present invention relates to crop extractive technique fields, more particularly, to a kind of extraction side of flavonols component in roundpod jute leaf
Method and its extract and application.
Background technique
Jute belongs to annual dicotyledon, and blade is oval or lanceolar, and color is green or blush, contains
The chemical analysis such as flavonols, common flavonols have the components such as isoquercitin, Quercetin and Kaempferol, have anti-inflammatory, spasmolysis,
The important medical value such as relieving cough and asthma.
How above-mentioned flavonols component is extracted from jute, it is of crucial importance to deep development jute crop.
In view of this, the present invention is specifically proposed.
Summary of the invention
The first object of the present invention is to provide a kind of extracting method of flavonols component in roundpod jute leaf, the extraction side
Method can isoquercitin, Quercetin and Kaempferol of the depth extraction into roundpod jute leaf, the extracting method high income, technique letter
It is single, mild condition.
The second object of the present invention is to provide a kind of extracting method using flavonols component in the roundpod jute leaf and mentions
The extract obtained, the content of isoquercitin, Quercetin and Kaempferol is high in the extract, living with excellent free radical
Property remove effect and tyrosinase activity inhibiting effect, anti-inflammatory, anti-oxidant and Bearberry Extract can be played.
The third object of the present invention is to provide a kind of application of extract, and the extract has excellent removing
The ability of free radical all has preferable elimination effect to hydroxy radical, DPPH free radical and ultra-oxygen anion free radical, is function
It can property food, beauty product offer basis.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
The extracting method of flavonols component, includes the following steps: in roundpod jute leaf
Refluxing extraction is carried out to roundpod jute leaf using the solution of alcohol-containing as extracting solution.
The present invention extracts roundpod jute leaf using the solution of alcohol-containing as extracting solution, mainly in alcohol dissolution roundpod jute leaf
Isoquercitin, Quercetin and Kaempferol, when avoiding extracting using pure water, water-soluble substances are largely dissolved out, caused by extract
Object complicated component, purification difficult, and the problem that activity is relatively low.
Preferably, in the extracting solution, the volume fraction of alcohol is 60-90%.
In the present invention, in extracting solution, the volume fraction of alcohol is particularly significant for extraction process.When the volume fraction of alcohol is upper
It states in range, dissolution can be avoided water-soluble while guaranteeing to extract isoquercitin in roundpod jute leaf, Quercetin and Kaempferol
Substance keeps activity relatively low and increases purification difficulty.If the volume fraction of alcohol is too low, water-soluble substances are dissolved out in extract
Excessively, extract activity is relatively low, and causes difficulty to purification；Substance if the volume fraction of alcohol is excessively high, in extract
Structure is more easily damaged, so that active material is reduced.
Also, said extracted liquid is used, the isoquercitin, Quercetin and Kaempferol of specific quantity, three association can be extracted
There is different effects with cooperation.
Preferably, the solution of the alcohol-containing is the aqueous solution of alcohol-containing.
Preferably, the alcohol includes methanol and ethyl alcohol.It is furthermore preferred that the alcohol is methanol.
Dissolution isoquercitin, Quercetin and Kaempferol can be taken into account using methanol and ethyl alcohol, dissolve out a certain amount of isoquercitrin
After element, Quercetin and Kaempferol, triple interaction, the inhibition for maximizing the effect and tyrosinase of removing free radical is made
With.
Wherein, using the aqueous solution of the methanol of the score containing certain volume as extracting solution, the extract extracted is to certainly
It is excellent by the Scavenging activity of base and significant to the inhibiting effect of tyrosinase.
Preferably, the extracting solution is the aqueous solution for the methanol that volume fraction is 70%.
Using said extracted liquid, roundpod jute leaf is extracted, can extract to obtain the isoquercitin of specific quantity, Quercetin and
Kaempferol, three's synergistic effect, so that reachable to the Scavenging activity of hydroxy radical, ultra-oxygen anion free radical and DPPH free radical
To 97.61%, 62.66% and 88.15%.
Preferably, the extracting solution is the aqueous solution for the methanol that volume fraction is 90%.
Using said extracted liquid, roundpod jute leaf is extracted, the isoquercitin, the quercitrin that obtain specific matching amount can be extracted
Element and Kaempferol, three's synergistic effect is best to the inhibiting effect of tyrosinase activity, can reach 85.56%.
Preferably, the ratio of the roundpod jute leaf and extracting solution be 1g ﹕ (20-40) mL, preferably 1g ﹕ (25-35) mL, it is more excellent
It is selected as 1 ﹕ 30mL.
Preferably, the temperature of the refluxing extraction is 70-90 DEG C, preferably 75-85 DEG C, more preferably 80 DEG C.
The temperature of the refluxing extraction influences entire extraction process, comprehensively considers dissolution object, and avoid the molten of impurity
Equal many factors out；By cooperation said extracted temperature and extracting solution ingredient, can take into account specific in extraction dissolution roundpod jute leaf
Ingredient isoquercitin, Quercetin and Kaempferol, and avoid dissolving out other water-soluble substances, reduce activity.
Preferably, the time of the refluxing extraction is 1-3h, preferably 1.5-2.5h, more preferably 2h.
Preferably, after extraction, it is centrifuged off precipitating, collects liquid.It is furthermore preferred that the revolving speed of the centrifugation is 6000-
10000rpm/min, preferably 8000rpm/min；The time of the centrifugation is 5-15min, preferably 10min.
The present invention synchronizes by the way that specific testing conditions are arranged and detects isoquercitin in extract, Quercetin and kaempferia galamga
The content of phenol.Specifically, the phosphoric acid that mobile phase is the methanol that volume ratio is 55 ﹕ 45 and mass fraction is 0.5%；Flow velocity is
0.8mL/min, column temperature: 25 DEG C；Ultraviolet detection wavelength: 360nm；Sample volume: 20 μ L.
By external standard method, the isoquercitin of certain proportion and concentration, the hybrid standard liquid of Quercetin and Kaempferol are prepared, is adopted
It is measured with identical HPLC testing conditions, calculates the content of isoquercitin in extract, Quercetin and Kaempferol.
The present invention also provides what a kind of extracting method using flavonols component in the roundpod jute leaf was extracted to mention
Take object.
It include isoquercitin, Quercetin and Kaempferol in the extract.
Wherein, the concentration of the isoquercitin is 30-93 μ g/mL, and the concentration of the Quercetin is 1-5 μ g/mL, the mountain
How the concentration of phenol is 0.1-4 μ g/mL.
Said extracted object, for tyrosinase activity inhibiting rate up to 85.56%, to hydroxy radical, superoxide anion
The Scavenging activity of free radical and DPPH free radical can reach 97.61%, 62.66% and 88.15%, have excellent inhibition junket ammonia
The activity of sour enzyme and the effect of free-radical scavenging activity.
The present invention also provides a kind of application of extract in terms of removing free radical.
Preferably, the free radical include one of hydroxy radical, ultra-oxygen anion free radical and DPPH free radical or
It is a variety of.
Preferably, application of the extract in the health food and/or cosmetics that free radical is removed in preparation.
Extract can assign food and/or makeup as active constituent as described in the addition in food and/or cosmetics
The effect of the certain removing free radical of product.
Compared with prior art, the invention has the benefit that
(1) extracting method of the present invention, being capable of isoquercitin, Quercetin and kaempferia galamga of the depth extraction into roundpod jute leaf
Phenol, high income, simple process, mild condition；
(2) extracting method of the present invention, can take into account the isoquercitin extracted from roundpod jute leaf, Quercetin and
The amount of Kaempferol, so that the concentration of the isoquercitin is 30-93 μ g/mL, the concentration of the Quercetin is 1-5 μ g/mL, described
The concentration of Kaempferol is 0.1-4 μ g/mL；
(3) extract extracted using extracting method of the present invention, passes through the isoquercitin of specific quantity, quercitrin
Element and Kaempferol three synergistic effect, ability and tyrosinase activity inhibiting effect with excellent removing free radical are right
Hydroxy radical, DPPH free radical and ultra-oxygen anion free radical all have preferable elimination effect；
(4) extract of the present invention, which can be used for preparing, has effects that remove free radical and inhibit tyrosinase activity
The health food and/or cosmetics of effect.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 provides the extraction obtained with the extraction with aqueous solution roundpod jute leaf of the alcohol of the score containing different volumes for the embodiment of the present invention
Clearance rate of the object to hydroxy radical；
Fig. 2 provides the extraction obtained with the extraction with aqueous solution roundpod jute leaf of the alcohol of the score containing different volumes for the embodiment of the present invention
Clearance rate of the object to ultra-oxygen anion free radical；
Fig. 3 provides the extraction obtained with the extraction with aqueous solution roundpod jute leaf of the alcohol of the score containing different volumes for the embodiment of the present invention
Clearance rate of the object to DPPH free radical；
Fig. 4 provides the extraction obtained with the extraction with aqueous solution roundpod jute leaf of the alcohol of the score containing different volumes for the embodiment of the present invention
Inhibiting rate of the object to tyrosinase activity.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with the drawings and specific embodiments, but
Be it will be understood to those of skill in the art that it is following described embodiments are some of the embodiments of the present invention, rather than it is whole
Embodiment is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.Based on the embodiments of the present invention, ability
Domain those of ordinary skill every other embodiment obtained without making creative work, belongs to guarantor of the present invention
The range of shield.The person that is not specified actual conditions in embodiment, carries out according to conventional conditions or manufacturer's recommended conditions.Agents useful for same
Or production firm person is not specified in instrument, is the conventional products that can be obtained by commercially available purchase.
The roundpod jute leaf of various embodiments of the present invention picks the roundpod jute leaf planted in Changsha Experimental Base, long fruit kind, leaf age
60d。
The part device information that the present invention uses is as follows:
Liquid chromatograph: Dionex Ultimate 3000；
C18 column: hermo Hypersil BDS C18 column (250mm × 4.6mm, 5 μm)；
UV detector: VWD-3400 UV detector.
Embodiment 1
A kind of extracting method of flavonols component in roundpod jute leaf is present embodiments provided, steps are as follows:
(1) roundpod jute leaf is cleaned, roundpod jute leaf powder is made in 50 DEG C of drying, crushing, and the average grain diameter of the roundpod jute leaf powder is
0.35±0.02mm；
(2) it weighs 1g roundpod jute leaf powder and sets the aqueous solution for being placed in the methanol that 30mL volume fraction is 70% in a round bottom flask
In, after 80 DEG C of condition heating and refluxing extraction 2h, it is cooled to room temperature；
(3) substance after extraction is centrifuged 10min in 8000r/min, removes precipitating, collect liquid to get extract.
Embodiment 2
A kind of extracting method of flavonols component in roundpod jute leaf is present embodiments provided, steps are as follows:
(1) roundpod jute leaf is cleaned, roundpod jute leaf powder is made in 50 DEG C of drying, crushing, and the average grain diameter of the roundpod jute leaf powder is
0.35±0.02mm；
(2) it weighs 1g roundpod jute leaf powder and sets the aqueous solution for being placed in the methanol that 30mL volume fraction is 60% in a round bottom flask
In, after 80 DEG C of condition heating and refluxing extraction 2h, it is cooled to room temperature；
(3) substance after extraction is centrifuged 10min in 8000r/min, removes precipitating, collect liquid to get extract.
Embodiment 3
A kind of extracting method of flavonols component in roundpod jute leaf is present embodiments provided, steps are as follows:
(1) roundpod jute leaf is cleaned, roundpod jute leaf powder is made in 50 DEG C of drying, crushing, and the average grain diameter of the roundpod jute leaf powder is
0.35±0.02mm；
(2) it weighs 1g roundpod jute leaf powder and sets the aqueous solution for being placed in the methanol that 30mL volume fraction is 80% in a round bottom flask
In, after 80 DEG C of condition heating and refluxing extraction 2h, it is cooled to room temperature；
(3) substance after extraction is centrifuged 10min in 8000r/min, removes precipitating, collect liquid to get extract.
Embodiment 4
A kind of extracting method of flavonols component in roundpod jute leaf is present embodiments provided, steps are as follows:
(1) roundpod jute leaf is cleaned, roundpod jute leaf powder is made in 50 DEG C of drying, crushing, and the average grain diameter of the roundpod jute leaf powder is
0.35±0.02mm；
(2) it weighs 1g roundpod jute leaf powder and sets the aqueous solution for being placed in the methanol that 30mL volume fraction is 90% in a round bottom flask
In, after 80 DEG C of condition heating and refluxing extraction 2h, it is cooled to room temperature；
(3) substance after extraction is centrifuged 10min in 8000r/min, removes precipitating, collect liquid to get extract.
Embodiment 5
A kind of extracting method of flavonols component in roundpod jute leaf is present embodiments provided, steps are as follows:
(1) roundpod jute leaf is cleaned, roundpod jute leaf powder is made in 50 DEG C of drying, crushing, and the average grain diameter of the roundpod jute leaf powder is
0.35±0.02mm；
(2) it weighs 1g roundpod jute leaf powder and sets the aqueous solution for being placed in the ethyl alcohol that 30mL volume fraction is 60% in a round bottom flask
In, after 80 DEG C of condition heating and refluxing extraction 2h, it is cooled to room temperature；
(3) substance after extraction is centrifuged 10min in 8000r/min, removes precipitating, collect liquid to get extract.
Embodiment 6
A kind of extracting method of flavonols component in roundpod jute leaf is present embodiments provided, steps are as follows:
(1) roundpod jute leaf is cleaned, roundpod jute leaf powder is made in 50 DEG C of drying, crushing, and the average grain diameter of the roundpod jute leaf powder is
0.35±0.02mm；
(2) it weighs 1g roundpod jute leaf powder and sets the aqueous solution for being placed in the ethyl alcohol that 30mL volume fraction is 70% in a round bottom flask
In, after 80 DEG C of condition heating and refluxing extraction 2h, it is cooled to room temperature；
(3) substance after extraction is centrifuged 10min in 8000r/min, removes precipitating, collect liquid to get extract.
Embodiment 7
A kind of extracting method of flavonols component in roundpod jute leaf is present embodiments provided, steps are as follows:
(1) roundpod jute leaf is cleaned, roundpod jute leaf powder is made in 50 DEG C of drying, crushing, and the average grain diameter of the roundpod jute leaf powder is
0.35±0.02mm；
(2) it weighs 1g roundpod jute leaf powder and sets the aqueous solution for being placed in the ethyl alcohol that 30mL volume fraction is 80% in a round bottom flask
In, after 80 DEG C of condition heating and refluxing extraction 2h, it is cooled to room temperature；
(3) substance after extraction is centrifuged 10min in 8000r/min, removes precipitating, collect liquid to get extract.
Embodiment 8
A kind of extracting method of flavonols component in roundpod jute leaf is present embodiments provided, steps are as follows:
(1) roundpod jute leaf is cleaned, roundpod jute leaf powder is made in 50 DEG C of drying, crushing, and the average grain diameter of the roundpod jute leaf powder is
0.35±0.02mm；
(2) it weighs 1g roundpod jute leaf powder and sets the aqueous solution for being placed in the ethyl alcohol that 30mL volume fraction is 90% in a round bottom flask
In, after 80 DEG C of condition heating and refluxing extraction 2h, it is cooled to room temperature；
(3) substance after extraction is centrifuged 10min in 8000r/min, removes precipitating, collect liquid to get extract.
Comparative example 1
The preparation method of 1 reference implementation example 1 of comparative example, difference are: using pure water as extracting solution, Extracting temperature 80
℃。
Experimental example 1
For the difference of comparative illustration various embodiments of the present invention and the extracting method of comparative example, to using each embodiment and ratio
The ingredient of the extract extracted compared with the extracting method of example is tested, and test result is shown in Table 1, and test method is as follows:
Hybrid standard liquid is prepared: accurately weighing isoquercitin 2.2mg, Quercetin 2.0mg, Kaempferol 1.8mg, mixing, first
Alcoholic solution ultrasonic dissolution, is settled to 25mL, 0.45 μm of membrane filtration, and appropriate extension rate is used as HPLC and tests and analyzes control；
The equation of linear regression difference of the peak area Y that titer is tested and concentration X is as follows:
Isoquercitin: Y=1.1923X+0.9108, R2=0.9965；
Quercetin: Y=0.7164X+0.3155, R2=0.9990；
Kaempferol: Y=0.8433X+0.3758, R2=0.9991.
The extract for taking each embodiment and comparative example to be prepared respectively takes 0.45 μm of membrane filtration of 1mL, using HPLC
It tests and analyzes；
HPLC testing conditions: mobile phase: V (methanol) ﹕ V (0.5% phosphoric acid)=55 ﹕ 45；Flow velocity: 0.8mL/min；Column temperature:
25℃；Ultraviolet detection wavelength: 360nm；Sample volume: 20 μ L.
Constituent content result in the extract of 1 different disposal of table
Experimental example 2
For the difference of comparative illustration various embodiments of the present invention and the extracting method of comparative example, to using each embodiment and ratio
The clearance rate of free radical is tested compared with the extract that the extracting method of example is extracted, test result such as Fig. 1-3 and table 2
Shown, test method is as follows:
The active measuring method of scavenging hydroxyl: the extract 2mL for taking each embodiment and comparative example to be prepared respectively
In test tube, the FeSO of 6mmol/L is sequentially added4, 6mmol/L H2O2Each 2mL of solution, shakes up, is stored at room temperature 10min；Again plus
Enter 6mmol/L salicylic acid solution 2mL, shake up, stands 30min, survey absorbance at 510nm；Blank control is set simultaneously；
Clearance rate: Scavenging action to hydroxyl free radical (%)=[1- (Ai-Aj)/A0] × 100% is calculated according to formula
Wherein, Ai FeSO4+H2O2+ extract sample+each 2mL of salicylic acid absorbance；Aj is FeSO4+H2O2+ sample+
The absorbance of each 2mL of water；A0 is FeSO4+H2O2The absorbance of each 2mL of+water+salicylic acid.
It removes the active measuring method of ultra-oxygen anion free radical: taking what each embodiment and comparative example be prepared to mention respectively
It takes object 0.2mL to be added into the Tris-HCl buffer (50mmol/L, pH=8.20) equipped with 5.7mL, is kept the temperature in 25 DEG C
Then 10min is added 0.1mL pyrogallol (6mmol/L, being preheated to 25 DEG C), uses spectrophotometry after shaking up at once
Absorbance when meter measurement reaction 1min at 320nm；Blank control is set simultaneously；
Clearance rate: ultra-oxygen anion free radical clearance rate (%)=[(Ae-As)/Ae] × 100% is calculated according to formula
Wherein, As is absorbance when Tris-HCl+ sample+Pyrogallol determination reacts 1min；Ae is Tris-HCl+ water
+ Pyrogallol determination reacts absorbance when 1min.
The measuring method of free radical scavenging: the extract for taking each embodiment and comparative example to be prepared respectively
In DPPH (hexichol is for bitter taste hydrazides free radical) liquid of 1mL to the 0.2mmol/L that 1mL is housed, 30min, 517nm are stood at room temperature
Place surveys absorbance；Blank control is set simultaneously；
Clearance rate: DPPH free radical scavenging activity (%)=[1- (Am-An)/Ac] × 100% is calculated according to formula
Wherein, Am is the absorbance of 1mLDPPH+1mL sample；An is the absorbance of 1mL dehydrated alcohol+1mL sample；Ac is
The absorbance of 1mLDPPH+1mL dehydrated alcohol.
The free-radical scavenging activity test result of the extract of 2 different disposal of table
| Number | Scavenging action to hydroxyl free radical (%) | DPPH clearance rate (%) | Superoxide anion clearance rate (%) |
| Embodiment 1 | 97.61 | 88.15 | 62.66 |
| Embodiment 2 | 86.23 | 84.74 | 28.21 |
| Embodiment 3 | 88.08 | 86.19 | 20.91 |
| Embodiment 4 | 66.07 | 84.50 | 6.42 |
| Embodiment 5 | 55.74 | 82.02 | 41.90 |
| Embodiment 6 | 95.80 | 85.27 | 43.61 |
| Embodiment 7 | 62.92 | 88.63 | 35.21 |
| Embodiment 8 | 78.56 | 85.39 | 46.26 |
| Comparative example 1 | 95.96 | 84.84 | 1.35 |
Experimental example 3
For the difference of comparative illustration various embodiments of the present invention and the extracting method of comparative example, to using each embodiment and ratio
The inhibiting rate of tyrosinase activity is tested compared with the extract that the extracting method of example is extracted, test result such as Fig. 4 and
Shown in table 4, test method is as follows:
L-tyrosine (1.5mmol/L), phosphate buffer PBS (0.2mol/L, pH are measured according to using in table 3 respectively
=6.8) and the obtained extract sample of each embodiment and comparative example is managed in (T1, T2, T3, T4) in 4 EP, is mixed, 37 DEG C of perseverances
It after warm 10min, is separately added into T2, T4 tyrosinase (200U/mL), reacts 10min, survey absorbance at 475nm.
3 tyrosinase activity of table inhibits reaction solution when measurement to form
Inhibitory activity against tyrosinase, inhibiting rate=[1- (AT4-AT3)/(AT2-AT1)] × 100% are calculated according to formula
Wherein, AT1 is the absorbance for the reaction solution for not being loaded product and not adding tyrosinase；AT2 is not to be loaded product and plus junket
The absorbance of the reaction solution of propylhomoserin enzyme；AT3 is sample-adding product and does not add the absorbance of the reaction solution of tyrosinase；AT4 is sample-adding product
With the absorbance for the reaction solution for adding tyrosinase.
The extract of 4 different disposal of table inhibits tyrosinase activity test result
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (10)
1. the extracting method of flavonols component in roundpod jute leaf, which comprises the steps of:
Refluxing extraction is carried out to roundpod jute leaf using the solution of alcohol-containing as extracting solution.
2. the extracting method of flavonols component in roundpod jute leaf according to claim 1, which is characterized in that the extracting solution
In, the volume fraction of alcohol is 60-90%.
3. the extracting method of flavonols component in roundpod jute leaf according to claim 1, which is characterized in that preferred, institute
Stating alcohol includes methanol and ethyl alcohol；
Preferably, the solution of the alcohol-containing is the aqueous solution of alcohol-containing.
4. the extracting method of flavonols component in roundpod jute leaf according to claim 1, which is characterized in that the extracting solution
For the aqueous solution for the methanol that volume fraction is 70%.
5. the extracting method of flavonols component in roundpod jute leaf according to claim 1, which is characterized in that the roundpod jute leaf
Ratio with extracting solution is 1g ﹕ (20-40) mL；
Preferably, the ratio of the roundpod jute leaf and extracting solution is 1g ﹕ (25-35) mL.
6. the extracting method of flavonols component in roundpod jute leaf according to claim 1, which is characterized in that the reflux mentions
The temperature taken is 70-90 DEG C；
Preferably, the temperature of the refluxing extraction is 75-85 DEG C；
Preferably, the time of the refluxing extraction is 1-3h；
Preferably, after extraction, it is centrifuged off precipitating, collects liquid；
It is furthermore preferred that the revolving speed of the centrifugation is 6000-10000rpm/min, the time of the centrifugation is 5-15min.
7. the extraction extracted using the extracting method of flavonols component in roundpod jute leaf described in any one of claims 1-6
Object；
It preferably, include isoquercitin, Quercetin and Kaempferol in the extract.
8. the extract that the extracting method of flavonols component is extracted in roundpod jute leaf according to claim 7, special
Sign is that the concentration of the isoquercitin is 30-93 μ g/mL, and the concentration of the Quercetin is 1-5 μ g/mL, the Kaempferol
Concentration is 0.1-4 μ g/mL.
9. application of the extract as claimed in claim 7 in terms of removing free radical；
Preferably, the free radical includes one of hydroxy radical, ultra-oxygen anion free radical and DPPH free radical or a variety of.
10. application of the extract according to claim 9 in terms of removing free radical, which is characterized in that the extract
Application in the health food and/or cosmetics that free radical is removed in preparation.
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| CN103300278A (en) * | 2012-03-08 | 2013-09-18 | 三得利控股株式会社 | Composition comprising imidazole peptide and quercetin glycoside |
| CN107949394A (en) * | 2015-08-19 | 2018-04-20 | 韩国生命工学研究院 | It is used to prevent or treats metabolic syndrome or for oxidation resistant composition comprising black soya bean leaf extract and from its separated flavonol glycosides is as active component |
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2018
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| CN101690739A (en) * | 2009-06-30 | 2010-04-07 | 长春师范学院 | Preparation method of dogbane leaf extractive |
| CN101940605A (en) * | 2010-08-30 | 2011-01-12 | 周小江 | Chinese loropetalum leaf total flavonol extract and medical application thereof |
| CN102028724A (en) * | 2010-12-09 | 2011-04-27 | 江南大学 | Method for preparing total flavone extracts from roundpod jute leaves |
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| CN107949394A (en) * | 2015-08-19 | 2018-04-20 | 韩国生命工学研究院 | It is used to prevent or treats metabolic syndrome or for oxidation resistant composition comprising black soya bean leaf extract and from its separated flavonol glycosides is as active component |
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