CN108918843A - Prosthese aseptic loosening bone dissolves semi-quantitative detection method - Google Patents
Prosthese aseptic loosening bone dissolves semi-quantitative detection method Download PDFInfo
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- CN108918843A CN108918843A CN201810670495.7A CN201810670495A CN108918843A CN 108918843 A CN108918843 A CN 108918843A CN 201810670495 A CN201810670495 A CN 201810670495A CN 108918843 A CN108918843 A CN 108918843A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Abstract
The invention discloses a kind of prosthese aseptic loosening bones to dissolve semi-quantitative detection method, includes the following steps：S1. cell at the artificial limitans tissue of joint replacement patient's prosthese installation site is taken, is arranged to control group and experimental group, is all made of conditioned medium and is cultivated, enzyme is added dropwise after cultivating 6~15d in control group, observes the variation of TRAP positive multinucleated cells and counting.The present invention obtains the index that multiple pairs of prosthese aseptic loosenings have a major impact effect, the postoperative prosthese aseptic loosening of joint prosthesis can be early diagnosed, it is small to the wound of patient, it is easy to operate, interpretation of result is simple, good clinic popularization and application values, are of great significance for guiding clinical treatment.
Description
Technical field
The present invention relates to artificial joint replacement technical fields, more specifically, are related to a kind of prosthese aseptic loosening bone
Dissolve semi-quantitative detection method.
Background technique
Joint replacement is made using materials such as metal, high molecular polythene, ceramics according to formation of human synovial etc.
At artificial joint prosthesis, and it is implanted into human body by surgical technic and replaces diseased joints.Artificial joint replacement is that treatment bone closes
The effective ways for saving disease, are the critical treatment means of joint whole Terminal Disease and femoral neck fracture in elders.But artificial pass
Section material, modus operandi, individual patients factor etc. will affect the service life of joint prosthesis.Joint prosthesis is caused to use the longevity
Ordering shorter reason has very much, and summing up mainly has mechanical factor and biological factor.Wherein, biological factor is mainly people
Work joint wear product (metallic particles, metal ion etc.) can stimulate proinflammatory cytokine secretion bone resorption sex factor such as TNF-α, IL-6
It stimulates osteocyte to generate bone dissolution Deng, bone resorption sex factor, leads to prosthese aseptic loosening.At present generally between 10~15 years,
It would have to manually be overhauled.
Joint replacement is one of most successful orthopaedic surgery in the world, it is intended to promote the quality of life of patient.With
Social development and aging of population process accelerate, more and more patients select joint replacements.Prosthese aseptic loosening is
The operation major complications, and cause the true cause of disability rate height and both expensive.Therefore, implantation material aseptic loosening
Belong to the hot and difficult issue problem of medical domain research.
The aseptic loosening of prosthese, by multifactor impact.Aseptic loosening caused by Periprosthetic bone dissolves is artificial
One of postoperative most common failure cause of joint replacement, at present still not to the specific mechanism of aseptic loosening of prosthesis
It is fully apparent from, it may be possible to by many factors, such as wear particle, metal ion, fine motion, stress shielding, high fluid pressure factor
Cause, the Periprosthetic bone dissolution of wear debris induction is considered as the main reason for causing prosthese aseptic loosening.
The early diagnosis of prosthese aseptic loosening is conducive to early treatment, and existing detection method is detected dependent on iconography
Means are early diagnosed, such as X-ray, CT scan etc..X-ray examination is the dissolution of evaluation Periprosthetic bone and prosthetic loosening
Important method, but for without obvious bone dissolution sign prosthetic loosening be generally difficult to make diagnosis.Prosthese aseptic loosening mesh
Before only rely on imageological examination and occur clinical symptoms just carry out diagnosis and Severity, susceptibility is not high enough, accurately
It spends low, is not able to satisfy clinical diagnosis needs.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of prosthese aseptic loosening bones to dissolve sxemiquantitative
Detection method, susceptibility is high, has important clinical value to early diagnosis aseptic prosthetic loosening, energy half-quantitative detection bone is molten
Situation is solved, small to the wound of patient, easy to operate, interpretation of result is simple, good clinic popularization and application values, for
Guiding clinical treatment is of great significance.
The purpose of the present invention is achieved through the following technical solutions：A kind of prosthese aseptic loosening bone dissolution sxemiquantitative
Detection method, including：
S1, cell at the artificial limitans tissue of joint replacement patient's prosthese installation site is taken, is arranged to control group and reality
Group is tested, conditioned medium is all made of and is cultivated, enzyme is added dropwise after cultivating 6~15d in control group, and observation TRAP positive multicore is thin
The variation and counting of born of the same parents；
S2, the experimental group after cultivating in conditioned medium in step S1 is divided into II group of experiment I group and experiment, will tested
I group is containing CoCl2Solution and CrCl324~30h of the continuous culture of mixing liquid culture medium relaying of solution；II group is tested in old terms
After cultivating 24~30h in culture medium, the conditioned medium that old terms culture medium is changed to not metal ion is continued into culture 48
~60h, cell and the supernatant collected experiment I group respectively, test II group；
The secretory volume of S3, the rank protein in the supernatant that detecting step S2 is obtained respectively, OPG albumen, experiment with computing I
Group, experiment II group in RANK/OPG ratio；
The peripheral blood mononuclear cells of joint replacement patient in S4, acquisition step S1 detects patient peripheral's blood monocyte
In CD14+CD16+Monocyte ratio；
S5, the peripheral blood mononuclear cells for acquiring normal person detect the CD14 in Normal human peripheral's blood monocyte+CD16+It is single
Nucleus ratio；
S6, the detected value obtained according to step S1, S3, S4, S5, Comprehensive Evaluation joint replacement patient's prosthese aseptic pine
Dynamic bone dissolves situation.
Preferably, enzyme described in step S1 is Tartrate resistant acid phosphatase.
Preferably, in step S2, CoCl in the mixing liquid culture medium2The concentration of solution is 10~15mg/L, CrCl3It is molten
The concentration of liquid is 15~20mg/L.
Preferably, in step S2, CoCl in the mixing liquid culture medium2The concentration of solution is 11~14mg/L, CrCl3It is molten
The concentration of liquid is 16~18mg/L.
Preferably, in step S2, CoCl in the mixing liquid culture medium2The concentration of solution is 13mg/L, CrCl3Solution
Concentration is 17mg/L.
Preferably, in step S3, using in RANK kit, OPG the kit supernatant that detecting step S2 is obtained respectively
Rank protein, OPG albumen secretory volume.
Preferably, in step S4, using the CD14 in FCM analysis instrument detection patient peripheral's blood monocyte+CD16+
Monocyte ratio.
Preferably, in step S5, using the CD14 in FCM analysis instrument detection Normal human peripheral's blood monocyte+
CD16+Monocyte ratio.
The beneficial effects of the invention are as follows：
(1)CD14+CD16+Monocyte, although not high in human body proportion, is ground as inflammatory mononuclear cells
Study carefully and show to play a significant role in inflammatory reaction, Normal human peripheral is detected using FCM analysis instrument in this programme
Blood CD14+CD16+Monocyte ratio, and as an index for judging bone dissolution situation, it is time-consuming compared to other, multiple
Miscellaneous immune detection can get by simple method in a relatively short period of time and judge that prosthese aseptic loosening bone dissolves feelings
One important indicator of condition, improves detection efficiency, this has important clinical valence for early diagnosis aseptic prosthetic loosening
Value；Also, since RANKL/RANK/OPG signal path is to adjust osteoclast differentiation and mature major avenues of approach, this programme
The ratio of RANK/OPG is obtained as another important indicator for judging prosthese aseptic loosening bone dissolution situation, in conjunction with CD14+CD16+Monocyte ratio index improves the accuracy of judgement degree of bone dissolution situation, and passes through setting multiple groups known concentration ratio
Example CoCl2Solution and CrCl3Solution is intervened, and detects the RANK/OPG ratio of intervention group and non-intervention group, Neng Gouban respectively
Quantitative detection bone dissolves situation, and susceptibility is high, and disability rate is low, and cost is at low cost, improves the quality of life of patient.
(2) present invention obtains the index that multiple pairs of prosthese aseptic loosenings have a major impact effect, can early diagnose artificial
Prosthese aseptic loosening after kan setsu waza, small to the wound of patient, easy to operate, interpretation of result is simple, and good clinic pushes away
Wide application value, is of great significance for guiding clinical treatment.
Specific embodiment
Technical solution of the present invention is detailed further below, but protection scope of the present invention is not limited to following institute
It states.All features disclosed in this specification, or implicit disclosed all methods or in the process the step of, in addition to mutually exclusive
Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated,
It is replaced by other equivalent or with similar purpose alternative features.That is, unless specifically stated, each feature is a system
Arrange an example in equivalent or similar characteristics.
Specific embodiments of the present invention are described more fully below, it should be noted that the embodiments described herein is served only for illustrating
Illustrate, is not intended to restrict the invention.In the following description, in order to provide a thorough understanding of the present invention, a large amount of spies are elaborated
Determine details.It will be apparent, however, to one skilled in the art that：This need not be carried out using these specific details
Invention.In other instances, in order to avoid obscuring the present invention, well known technology is not specifically described.
Embodiment 1
A kind of prosthese aseptic loosening bone dissolution semi-quantitative detection method, including：
S1, cell at the artificial limitans tissue of joint replacement patient's prosthese installation site is taken, is arranged to control group and reality
Group to be tested, conditioned medium is all made of and is cultivated, Tartrate resistant acid phosphatase is added dropwise after cultivating 12d in control group, it dyes,
Observe the variation of TRAP positive multinucleated cells and counting；
S2, the experimental group after cultivating in conditioned medium in step S1 is divided into II group of experiment I group and experiment, will tested
I group is containing CoCl2Solution and CrCl3The continuous culture 28h of mixing liquid culture medium relaying of solution；II group is tested in old terms culture
After cultivating 28h in base, the conditioned medium that old terms culture medium is changed to not metal ion is continued to cultivate 52h, is received respectively
Collection experiment I group, cell and the supernatant for testing II group；CoCl in the mixing liquid culture medium2The concentration of solution is 13mg/L,
CrCl3The concentration of solution is 17mg/L；
S3, be respectively adopted RANK kit, the rank protein in the supernatant that OPG kit detecting step S2 is obtained,
The secretory volume of OPG albumen, experiment with computing I group, experiment II group in RANK/OPG ratio；
The peripheral blood mononuclear cells of joint replacement patient in S4, acquisition step S1 is detected using FCM analysis instrument
CD14 in patient peripheral's blood monocyte+CD16+Monocyte ratio；
S5, the peripheral blood mononuclear cells for acquiring normal person detect normal human peripheral blood's monokaryon using FCM analysis instrument
CD14 in cell+CD16+Monocyte ratio；
S6, the detected value obtained according to step S1, S3, S4, S5, Comprehensive Evaluation joint replacement patient's prosthese aseptic pine
Dynamic bone dissolves situation.
Finally, whether being more than normal value, then judgment step by the ratio of the RANK/OPG calculated in first judgment step S3
S4 and S5 makees bone dissolution according to step S1 and qualitatively judges, and makees bone dissolution rational judgment according to step S3, S4, S5.
Embodiment 2
A kind of prosthese aseptic loosening bone dissolution semi-quantitative detection method, including：
S1, cell at the artificial limitans tissue of joint replacement patient's prosthese installation site is taken, is arranged to control group and reality
Group to be tested, conditioned medium is all made of and is cultivated, Tartrate resistant acid phosphatase is added dropwise after cultivating 15d in control group, it dyes,
Observe the variation of TRAP positive multinucleated cells and counting；
S2, the experimental group after cultivating in conditioned medium in step S1 is divided into II group of experiment I group and experiment, will tested
I group is containing CoCl2Solution and CrCl3The continuous culture of mixing liquid culture medium relaying of solution is for 24 hours；II group is tested in old terms culture
After cultivating 26h in base, the conditioned medium that old terms culture medium is changed to not metal ion is continued to cultivate 60h, is received respectively
Collection experiment I group, cell and the supernatant for testing II group；CoCl in the mixing liquid culture medium2The concentration of solution is 10mg/L,
CrCl3The concentration of solution is 15mg/L；
S3, be respectively adopted RANK kit, the rank protein in the supernatant that OPG kit detecting step S2 is obtained,
The secretory volume of OPG albumen, experiment with computing I group, experiment II group in RANK/OPG ratio；
The peripheral blood mononuclear cells of joint replacement patient in S4, acquisition step S1 is detected using FCM analysis instrument
CD14 in patient peripheral's blood monocyte+CD16+Monocyte ratio；
S5, the peripheral blood mononuclear cells for acquiring normal person detect normal human peripheral blood's monokaryon using FCM analysis instrument
CD14 in cell+CD16+Monocyte ratio；
S6, the detected value obtained according to step S1, S3, S4, S5, Comprehensive Evaluation joint replacement patient's prosthese aseptic pine
Dynamic bone dissolves situation.
Finally, whether being more than normal value, then judgment step by the ratio of the RANK/OPG calculated in first judgment step S3
S4 and S5 makees bone dissolution according to step S1 and qualitatively judges, and makees bone dissolution rational judgment according to step S3, S4, S5.
Embodiment 3
A kind of prosthese aseptic loosening bone dissolution semi-quantitative detection method, including：
S1, cell at the artificial limitans tissue of joint replacement patient's prosthese installation site is taken, is arranged to control group and reality
Group to be tested, conditioned medium is all made of and is cultivated, Tartrate resistant acid phosphatase is added dropwise after cultivating 8d in control group, it dyes,
Observe the variation of TRAP positive multinucleated cells and counting；
S2, the experimental group after cultivating in conditioned medium in step S1 is divided into II group of experiment I group and experiment, will tested
I group is containing CoCl2Solution and CrCl3The continuous culture 30h of mixing liquid culture medium relaying of solution；II group is tested in old terms culture
After cultivating 25h in base, the conditioned medium that old terms culture medium is changed to not metal ion is continued to cultivate 48h, is received respectively
Collection experiment I group, cell and the supernatant for testing II group；CoCl in the mixing liquid culture medium2The concentration of solution is 15mg/L,
CrCl3The concentration of solution is 18mg/L；
S3, be respectively adopted RANK kit, the rank protein in the supernatant that OPG kit detecting step S2 is obtained,
The secretory volume of OPG albumen, experiment with computing I group, experiment II group in RANK/OPG ratio；
The peripheral blood mononuclear cells of joint replacement patient in S4, acquisition step S1 is detected using FCM analysis instrument
CD14 in patient peripheral's blood monocyte+CD16+Monocyte ratio；
S5, the peripheral blood mononuclear cells for acquiring normal person detect normal human peripheral blood's monokaryon using FCM analysis instrument
CD14 in cell+CD16+Monocyte ratio；
S6, the detected value obtained according to step S1, S3, S4, S5, Comprehensive Evaluation joint replacement patient's prosthese aseptic pine
Dynamic bone dissolves situation.
Finally, whether being more than normal value, then judgment step by the ratio of the RANK/OPG calculated in first judgment step S3
S4 and S5 makees bone dissolution according to step S1 and qualitatively judges, and makees bone dissolution rational judgment according to step S3, S4, S5.
Embodiment 4
A kind of prosthese aseptic loosening bone dissolution semi-quantitative detection method, including：
S1, cell at the artificial limitans tissue of joint replacement patient's prosthese installation site is taken, is arranged to control group and reality
Group to be tested, conditioned medium is all made of and is cultivated, Tartrate resistant acid phosphatase is added dropwise after cultivating 13d in control group, it dyes,
Observe the variation of TRAP positive multinucleated cells and counting；
S2, the experimental group after cultivating in conditioned medium in step S1 is divided into II group of experiment I group and experiment, will tested
I group is containing CoCl2Solution and CrCl3The continuous culture 28h of mixing liquid culture medium relaying of solution；II group is tested in old terms culture
After cultivating 30h in base, the conditioned medium that old terms culture medium is changed to not metal ion is continued to cultivate 60h, is received respectively
Collection experiment I group, cell and the supernatant for testing II group；CoCl in the mixing liquid culture medium2The concentration of solution is 11mg/L,
CrCl3The concentration of solution is 16mg/L；
S3, be respectively adopted RANK kit, the rank protein in the supernatant that OPG kit detecting step S2 is obtained,
The secretory volume of OPG albumen, experiment with computing I group, experiment II group in RANK/OPG ratio；
The peripheral blood mononuclear cells of joint replacement patient in S4, acquisition step S1 is detected using FCM analysis instrument
CD14 in patient peripheral's blood monocyte+CD16+Monocyte ratio；
S5, the peripheral blood mononuclear cells for acquiring normal person detect normal human peripheral blood's monokaryon using FCM analysis instrument
CD14 in cell+CD16+Monocyte ratio；
S6, the detected value obtained according to step S1, S3, S4, S5, Comprehensive Evaluation joint replacement patient's prosthese aseptic pine
Dynamic bone dissolves situation.
Finally, whether being more than normal value, then judgment step by the ratio of the RANK/OPG calculated in first judgment step S3
S4 and S5 makees bone dissolution according to step S1 and qualitatively judges, and makees bone dissolution rational judgment according to step S3, S4, S5.
Embodiment 5
A kind of prosthese aseptic loosening bone dissolution semi-quantitative detection method, including：
S1, cell at the artificial limitans tissue of joint replacement patient's prosthese installation site is taken, is arranged to control group and reality
Group to be tested, conditioned medium is all made of and is cultivated, Tartrate resistant acid phosphatase is added dropwise after cultivating 14d in control group, it dyes,
Observe the variation of TRAP positive multinucleated cells and counting；
S2, the experimental group after cultivating in conditioned medium in step S1 is divided into II group of experiment I group and experiment, will tested
I group is containing CoCl2Solution and CrCl3The continuous culture of mixing liquid culture medium relaying of solution is for 24 hours；II group is tested in old terms culture
After cultivating 29h in base, the conditioned medium that old terms culture medium is changed to not metal ion is continued to cultivate 55h, is received respectively
Collection experiment I group, cell and the supernatant for testing II group；CoCl in the mixing liquid culture medium2The concentration of solution is 14mg/L,
CrCl3The concentration of solution is 18mg/L；
S3, be respectively adopted RANK kit, the rank protein in the supernatant that OPG kit detecting step S2 is obtained,
The secretory volume of OPG albumen, experiment with computing I group, experiment II group in RANK/OPG ratio；
The peripheral blood mononuclear cells of joint replacement patient in S4, acquisition step S1 is detected using FCM analysis instrument
CD14 in patient peripheral's blood monocyte+CD16+Monocyte ratio；
S5, the peripheral blood mononuclear cells for acquiring normal person detect normal human peripheral blood's monokaryon using FCM analysis instrument
CD14 in cell+CD16+Monocyte ratio；
S6, the detected value obtained according to step S1, S3, S4, S5, Comprehensive Evaluation joint replacement patient's prosthese aseptic pine
Dynamic bone dissolves situation.
Finally, whether being more than normal value, then judgment step by the ratio of the RANK/OPG calculated in first judgment step S3
S4 and S5 makees bone dissolution according to step S1 and qualitatively judges, and makees bone dissolution rational judgment according to step S3, S4, S5.
Embodiment 6
A kind of prosthese aseptic loosening bone dissolution semi-quantitative detection method, including：
S1, cell at the artificial limitans tissue of joint replacement patient's prosthese installation site is taken, is arranged to control group and reality
Group to be tested, conditioned medium is all made of and is cultivated, Tartrate resistant acid phosphatase is added dropwise after cultivating 10d in control group, it dyes,
Observe the variation of TRAP positive multinucleated cells and counting；
S2, the experimental group after cultivating in conditioned medium in step S1 is divided into II group of experiment I group and experiment, will tested
I group is containing CoCl2Solution and CrCl3The continuous culture 27h of mixing liquid culture medium relaying of solution；II group is tested in old terms culture
After cultivating 26h in base, the conditioned medium that old terms culture medium is changed to not metal ion is continued to cultivate 58h, is received respectively
Collection experiment I group, cell and the supernatant for testing II group；CoCl in the mixing liquid culture medium2The concentration of solution is 13mg/L,
CrCl3The concentration of solution is 18mg/L；
S3, be respectively adopted RANK kit, the rank protein in the supernatant that OPG kit detecting step S2 is obtained,
The secretory volume of OPG albumen, experiment with computing I group, experiment II group in RANK/OPG ratio；
The peripheral blood mononuclear cells of joint replacement patient in S4, acquisition step S1 is detected using FCM analysis instrument
CD14 in patient peripheral's blood monocyte+CD16+Monocyte ratio；
S5, the peripheral blood mononuclear cells for acquiring normal person detect normal human peripheral blood's monokaryon using FCM analysis instrument
CD14 in cell+CD16+Monocyte ratio；
S6, the detected value obtained according to step S1, S3, S4, S5, Comprehensive Evaluation joint replacement patient's prosthese aseptic pine
Dynamic bone dissolves situation.
Finally, whether being more than normal value, then judgment step by the ratio of the RANK/OPG calculated in first judgment step S3
S4 and S5 makees bone dissolution according to step S1 and qualitatively judges, and makees bone dissolution rational judgment according to step S3, S4, S5.
The above is only a preferred embodiment of the present invention, it should be understood that the present invention is not limited to described herein
Form should not be regarded as an exclusion of other examples, and can be used for other combinations, modifications, and environments, and can be at this
In the text contemplated scope, modifications can be made through the above teachings or related fields of technology or knowledge.And those skilled in the art institute into
Capable modifications and changes do not depart from the spirit and scope of the present invention, then all should be in the protection scope of appended claims of the present invention
It is interior.
Claims (8)
1. a kind of prosthese aseptic loosening bone dissolves semi-quantitative detection method, which is characterized in that including：
S1, cell at the artificial limitans tissue of joint replacement patient's prosthese installation site is taken, is arranged to control group and experimental group,
It is all made of conditioned medium to be cultivated, enzyme is added dropwise after cultivating 6~15d in control group, observes the change of TRAP positive multinucleated cells
Change and counts；
S2, the experimental group after cultivating in conditioned medium in step S1 is divided into II group of experiment I group and experiment, I group will be tested
Containing CoCl2Solution and CrCl324~30h of the continuous culture of mixing liquid culture medium relaying of solution；II group is tested in old terms culture
In base cultivate 24~30h after, by the conditioned medium that old terms culture medium is changed to not metal ion continue culture 48~
60h, cell and the supernatant collected experiment I group respectively, test II group；
The secretory volume of S3, the rank protein in the supernatant that detecting step S2 is obtained respectively, OPG albumen, experiment with computing I group, reality
Test the ratio of the RANK/OPG in II group；
The peripheral blood mononuclear cells of joint replacement patient in S4, acquisition step S1 detects in patient peripheral's blood monocyte
CD14+CD16+Monocyte ratio；
S5, the peripheral blood mononuclear cells for acquiring normal person detect the CD14 in Normal human peripheral's blood monocyte+CD16+Monokaryon is thin
Born of the same parents' ratio；
S6, the detected value obtained according to step S1, S3, S4, S5, Comprehensive Evaluation joint replacement patient's prosthese aseptic loosening
Bone dissolves situation.
2. prosthese aseptic loosening bone according to claim 1 dissolves semi-quantitative detection method, which is characterized in that step S1
Described in enzyme be Tartrate resistant acid phosphatase.
3. prosthese aseptic loosening bone according to claim 1 dissolves semi-quantitative detection method, which is characterized in that step S2
In, CoCl in the mixing liquid culture medium2The concentration of solution is 10~15mg/L, CrCl3The concentration of solution is 15~20mg/L.
4. prosthese aseptic loosening bone according to claim 1 or 3 dissolves semi-quantitative detection method, which is characterized in that step
In rapid S2, CoCl in the mixing liquid culture medium2The concentration of solution is 11~14mg/L, CrCl3The concentration of solution be 16~
18mg/L。
5. prosthese aseptic loosening bone according to claim 4 dissolves semi-quantitative detection method, which is characterized in that step S2
In, CoCl in the mixing liquid culture medium2The concentration of solution is 13mg/L, CrCl3The concentration of solution is 17mg/L.
6. prosthese aseptic loosening bone according to claim 1 dissolves semi-quantitative detection method, which is characterized in that step S3
In, using point of rank protein, OPG albumen in RANK kit, OPG the kit supernatant that detecting step S2 is obtained respectively
The amount of secreting.
7. prosthese aseptic loosening bone according to claim 1 dissolves semi-quantitative detection method, which is characterized in that step S4
In, using the CD14 in FCM analysis instrument detection patient peripheral's blood monocyte+CD16+Monocyte ratio.
8. prosthese aseptic loosening bone according to claim 1 dissolves semi-quantitative detection method, which is characterized in that step S5
In, using the CD14 in FCM analysis instrument detection Normal human peripheral's blood monocyte+CD16+Monocyte ratio.
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Citations (2)
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| US20120258123A1 (en) * | 2011-04-08 | 2012-10-11 | Zora Biosciences Oy | Biomarkers for sensitive detection of statin-induced muscle toxicity |
| CN103575877A (en) * | 2012-07-30 | 2014-02-12 | 上海交通大学医学院附属第九人民医院 | Kit for diagnosing artificial hip joint aseptic loosening |
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2018
- 2018-06-26 CN CN201810670495.7A patent/CN108918843A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120258123A1 (en) * | 2011-04-08 | 2012-10-11 | Zora Biosciences Oy | Biomarkers for sensitive detection of statin-induced muscle toxicity |
| CN103575877A (en) * | 2012-07-30 | 2014-02-12 | 上海交通大学医学院附属第九人民医院 | Kit for diagnosing artificial hip joint aseptic loosening |
Non-Patent Citations (2)
| Title |
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| 姚浩群: "金属离子与金属颗粒生物学活性实验研究", 《万方学位论文数据库》 * |
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