CN108918707A - A kind of method that liquid chromatograph mass spectrography measures glutaraldehyde content in water - Google Patents
A kind of method that liquid chromatograph mass spectrography measures glutaraldehyde content in water Download PDFInfo
- Publication number
- CN108918707A CN108918707A CN201810734386.7A CN201810734386A CN108918707A CN 108918707 A CN108918707 A CN 108918707A CN 201810734386 A CN201810734386 A CN 201810734386A CN 108918707 A CN108918707 A CN 108918707A
- Authority
- CN
- China
- Prior art keywords
- sample
- water
- glutaraldehyde
- measured
- mass spectrography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/067—Preparation by reaction, e.g. derivatising the sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
- G01N2030/146—Preparation by elimination of some components using membranes
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
A kind of method that liquid chromatograph mass spectrography measures glutaraldehyde content in water, it is characterised in that mainly include the following steps：Step 1: sample acquisition step：Obtain water sample to be measured；Step 2: pre-treatment step：Acetonitrile and 2,4-dinitrophenylhydrazine are added in water sample to be measured, mixes immediately, after room temperature reaction 30 minutes, obtains derivatization sample and is fitted into sample introduction bottle after membrane filtration, it is to be measured；Step 3: detecting step；Derivatization sample is subjected to liquid chromatography-mass spectrography detection；Step 4: calculating step：The content of glutaraldehyde in water sample to be measured is calculated.It solves the detection to glutaraldehyde content in source water and Drinking Water using the method for liquid chromatograph mass spectrography, there is simple and quick, high sensitivity, high repeatability and other advantages.
Description
Technical field
This application involves be glutaraldehyde content in a kind of liquid chromatograph mass spectrography measurement water method, belong to and drink
Water detection technique field.
Background technique
Glutaraldehyde is commonly used for disinfection sanitizer, organic synthesis intermediate, tanning extracts, oil-field flooding fungicide etc., to human body
Group is woven with moderate toxicity.Glutaraldehyde is one of the chemical substance of keyholed back plate at Britain's dangerous substance safety management, Britain's health at present
Safe administration department has formulated the safe contact standard of 0.2mg/kg for the occupation being necessarily exposed in glutaraldehyde working environment.
In European Union's daily necessities in the eco-label of unharmful substance, glutaraldehyde is listed in the toxic or nuisance being forbidden to use
Matter.《Standards for drinking water quality》（GB 5479-2006）Provide that its standard limited value is 0.07mg/L in appendix A.Currently, state
The standard method of glutaraldehyde in the inside and outside drinking water of detection not yet, existing report is primarily directed in thimerosal and leather penta 2
The detection of aldehyde, detection method mainly have titration and instrumental method.Titration is only applicable to the detection of high concentration glutaraldehyde solution, and
Titration end-point is not easy to grasp, and needs to configure pH meter to help directing terminal, and need using a large amount of hydroxylamine hydrochloride and second
Alcohol, there are certain influences for calibration of the by-product to glutaraldehyde concentration in reaction process.Such as following documents disclose correlator
Detection of the device method to glutaraldehyde.
Liaoning Normal University's journal (natural science edition), disclose within 2003 one it is entitled《Quantitative analysis by gas chromatography
The content of glutaraldehyde》Paper, author：Zhou Xiaoshuan, Xu Guiying.
Public Health and Preventive Medicine, disclose within 2011 one it is entitled《The measurement of gas-chromatography Internal standard curve method disappears
Glutaraldehyde in venom》Paper, author Wan Zhengyang.Using n,N-dimethylacetamide as internal standard, gas chromatography measurement disappears
Glutaraldehyde in venom, FFAP wide bore capillary column, FID detection, direct injected, sample volume 0.5 μ L, detection limit 0.04g/L.
Henan preventive medicine magazine, disclose within 2004 one it is entitled《High performance liquid chromatography quickly analyzes glutaraldehyde and disappears
The content of venom》Paper, author Song Xin, Dong Lianjie.Using the glutaraldehyde in liquid chromatography detection thimerosal, after membrane filtration
Direct injected, chromatographic column YWG-C18 column, the methanol of mobile phase 50%, UV detector, wavelength 235nm, 25 DEG C of column temperature, sample volume
10 μ L, concentration limit 5.0mg/L.
Leather science and engineering disclose one in 2012《Gas chromatography measures the glutaraldehyde content in leather》By
Text, author Yin Honglei, Mao Shulu, Dai Jinlan.Glutaraldehyde, chromatographic column are measured using methylene chloride extraction Gas Chromatography-mass Spectrometry
DB-5MS, detector FID or MS, 1 μ L, GC/MS detection limit 0.3mg/L, GC/FID detection limit 2.0mg/L of sample volume.
Document disclosed above is not directed to glutaraldehyde content in measurement water.
Summary of the invention
Technical problems to be solved in this application：How the technology of in source water and Drinking Water glutaraldehyde content is detected
Problem.
The technical solution that the application takes：A kind of method that liquid chromatograph mass spectrography measures glutaraldehyde content in water, it is main
Include the following steps：
Step 1: sample acquisition step：Obtain water sample to be measured；
Step 2: pre-treatment step：Acetonitrile and 2,4-dinitrophenylhydrazine are added in water sample to be measured, mixes immediately, reacts at room temperature
After 30 minutes, obtains derivatization sample and be fitted into sample introduction bottle after membrane filtration, it is to be measured；
Step 3: detecting step；By derivatization sample carry out liquid chromatography-mass spectrography detection, the liquid-phase condition selected for：Mobile phase
A is the pure water of the ammonium acetate containing 2.5mmol/L, and Mobile phase B is acetonitrile, chromatographic column C18, gradient elution, flow velocity 0.50mL/min,
45 DEG C of column temperature, 5 μ L of sample volume, mobile phase reference gradient is shown in Table 1：
1. mobile phase reference gradient of table
The Mass Spectrometry Conditions selected for：Electrospray ionisation source, negative ion mode is quantitative using multiple-reaction monitoring mode, parent ion/son
Ion is 459.0/182.1 and 459.0/163.0, wherein 182.1 be quantitative daughter ion, removing cluster voltage is -90 V, collision energy
For -23.8V and -25.2V, entrance potential is -10V, and collision exit potential is -12V, -4500 V of ion spray voltage, ion source
Temperature is 500 DEG C, and gas curtain gas velocity is 20psi, and collision gas flow velocity is 6psi, and atomization gas flow velocity is 50psi, secondary air speed
For 50psi；
Step 4: calculating step：The content of glutaraldehyde in water sample to be measured is calculated.
The advantages of the application：It is solved using the method for liquid chromatograph mass spectrography in source water and Drinking Water
The detection of glutaraldehyde content has simple and quick, high sensitivity, high repeatability and other advantages.
Detailed description of the invention
Fig. 1 is glutaraldehyde total ion current figure.
Fig. 2 is influence of the molar ratio of DNPH and glutaraldehyde to reaction result.
Fig. 3 is influence of the derivatization time to reaction result.
Specific embodiment
A kind of method that liquid chromatograph mass spectrography measures glutaraldehyde content in water, it is characterised in that mainly include as follows
Step：
Step 1: sample acquisition step：Obtain water sample to be measured；
Step 2: pre-treatment step：Acetonitrile and 2,4-dinitrophenylhydrazine are added in water sample to be measured, mixes immediately, reacts at room temperature
After 30 minutes, obtains derivatization sample and be fitted into sample introduction bottle after membrane filtration, it is to be measured；
Step 3: detecting step；By derivatization sample carry out liquid chromatography-mass spectrography detection, the liquid-phase condition selected for：Mobile phase
A is the pure water of the ammonium acetate containing 2.5mmol/L, and Mobile phase B is acetonitrile, chromatographic column C18, gradient elution, flow velocity 0.50mL/min,
45 DEG C of column temperature, 5 μ L of sample volume, mobile phase reference gradient is shown in Table 1：
1. mobile phase reference gradient of table
The Mass Spectrometry Conditions selected for：Electrospray ionisation source, negative ion mode is quantitative using multiple-reaction monitoring mode, parent ion/son
Ion is 459.0/182.1 and 459.0/163.0, wherein 182.1 be quantitative daughter ion, removing cluster voltage is -90 V, collision energy
For -23.8V and -25.2V, entrance potential is -10V, and collision exit potential is -12V, -4500 V of ion spray voltage, ion source
Temperature is 500 DEG C, and gas curtain gas velocity is 20psi, and collision gas flow velocity is 6psi, and atomization gas flow velocity is 50psi, secondary air speed
For 50psi；
Step 4: calculating step：The content of glutaraldehyde in water sample to be measured is calculated.
Preferably, it if water sample to be measured collected in sample acquisition step is tap water, then after obtaining water sample to be measured, also wraps
The step of including to water sample dechlorination to be measured processing, the dechlorination processing step, according to 1:2% ascorbic acid solution is added in 1000 ratio
It is handled.
In sample pre-treatments step：Water sample sampling amount is 200 μ L, and acetonitrile additional amount is 700 μ L, and 2,4-dinitrophenylhydrazine is dense
Degree is 0.12mmol/L, and additional amount is 100 μ L.
In sample pre-treatments step：In 0 ~ 100 μ g/L concentration range when measurement, to guarantee that derivatization is complete, 2,4- bis-
The molar ratio of nitrophenyl hydrazine and glutaraldehyde dosage need to be greater than 60.
Specific detection method and experimental data is described below.
One, material and method.
1, instrument and reagent.
Liquid chromatogram-tandem mass spectrum combined instrument：Ultrahigh-pressure liquid chromatograph model LC-30A（For example, SHIMADZU company
The liquid chromatogram of production-tandem mass spectrum combined instrument）, triple quadrupole mass spectrometer model API 4000+（For example, American AB
The triple quadrupole mass spectrometer of SCIEX company production）, it is furnished with the source ESI；Chromatographic column：GL InertSustainSwift C18
（1.9μm 2.1mm×100mm）；Pipettor：1000μL,100μL,200μL；Vortex mixer（For example, the industry of upper Nereid section has
The vortex mixer of limit company production）；Electronic balance：0.1mg.
Glutaraldehyde：50%（In water）, analyze pure；2,4 dinitrophenyl hydrazine（DNPH）：It analyzes pure；Glutaraldehyde-DNPH：100μ
G/ml, in acetonitrile；Acetonitrile：Chromatographically pure；Ammonium acetate：Chromatographically pure；Filter membrane：0.45 μm of hydrophilic PTFE syringe filter；Ascorbic acid：
It analyzes pure；Perchloric acid：It analyzes pure；Ultrapure water：18.2MΩ•cm.
2, standard solution and preparation of reagents.
The 2,4 dinitrophenyl hydrazine of 0.12mmol/L：2.3780g 2,4-dinitrophenylhydrazine is weighed, it is molten with 20% perchloric acid
Solution, and it is settled to 100mL, concentration 120mmol/L.100 μ L are drawn in 100mL volumetric flask, are settled to 20% perchloric acid
Scale, concentration 0.12mmol/L.
Glutaraldehyde standard solution：50% glutaraldehyde water solution is drawn into 100 μ L into 100mL volumetric flask, pure water is diluted to
Scale, concentration 500mg/L, as standard reserving solution.Then stock solution is used with the standard that ultrapure water is diluted to 500 μ g/L
The standard solution is further diluted according to requirement of experiment and obtains the standard series of various concentration by liquid.
3, instrument condition.
（1）Liquid-phase condition.
Mobile phase A is pure water（Ammonium acetate containing 2.5mmol/L）, Mobile phase B is acetonitrile, gradient elution, initial organic phase
Ratio 50%, flow velocity 0.50mL/min, 45 DEG C of column temperature, 5 μ L of sample volume.Mobile phase reference gradient is shown in Table 1.
1 mobile phase reference gradient of table.
（2）Mass Spectrometry Conditions.
ESI negative ion mode, using multiple-reaction monitoring（MRM）Mode is quantitative, and glutaraldehyde-DNPH parent ion/daughter ion is
459.0/182.1 and 459.0/163.0, remove cluster voltage（DP）For -90 V, collision energy（CE）For -23.8V and -25.2V, enter
Mouth voltage（EP）For -10V, exit potential is collided（CXP）For -12V, ion spray voltage（IS）- 4500 V, ion source temperature
It (TEM) is 500 DEG C, gas curtain gas velocity（CUR）For 20psi, collision gas flow velocity（CAD）For 6psi, atomization gas flow velocity（GS1）For
50psi, secondary air speed（GS2）For 50psi.Wherein, 182.1 be quantitative daughter ion.
4, the drafting of standard curve.
The standard curve that drafting low concentration and high concentration are applicable in respectively.Compound concentration 0,0.5,1.0,2.0,5.0,10,
25, the glutaraldehyde standard series of 50,70 μ g/L, according to carrying out analysis measurement after corresponding operating condition derivatization.Low concentration graticule
Range is 0.50-10.00 μ g/L, and high concentration graticule range is 5.00-70.00 μ g/L.
5, the acquisition and pre-treatment of sample.
The acquisition of sample：With cleaning, dry Brown Glass Brown glass bottles and jars only sampling.It is remaining due to containing in water when acquiring originally water sample
Chlorine is added ascorbic acid and carries out dechlorination processing after sampling（By 1：1000 are added 2% ascorbic acid solution）.
Sample pre-treatments：200 μ L water samples to be measured are drawn in vial, 700 μ L acetonitriles and 100 μ L are added
The 2,4-dinitrophenylhydrazine of 0.12mmol/L, mixes immediately, reacts at room temperature 30min.With 0.45 μm of PVDF membrane filtration, it is packed into
In 2mL sample injection bottle, analyzed to upper machine.
Two, conclusion.
1, the optimization of testing conditions.
Glutaraldehyde-the DNPH of 200 μ g/L is prepared with 50% acetonitrile solution, sample introduction is pumped using needle, establishes and optimizes mass spectrum
Condition.Liquid chromatogram is connected, optimizes liquid-phase condition by changing eluent gradient.Glutaraldehyde appearance time 2.79min, is shown in Fig. 1.
（1）The selection of mobile phase.
Acetonitrile-pure water, acetonitrile-pure water is respectively adopted（Ammonium acetate containing 2.5mmol/L）As mobile phase, gradient journey is set
Sequence is tested, as a result, it has been found that the response of target compound improves 1 times after the ammonium acetate of 2.5mmol/L is added in pure water.Finally
Select acetonitrile-pure water（Ammonium acetate containing 2.5mmol/L）As mobile phase.
（2）Influence of the dosage of DNPH to result.
By reaction equation it is found that 1 mole of glutaraldehyde needs 2 moles of DNPH reactions, derivating agent dosage is too small, can make to react endless
Entirely.Using the glutaraldehyde of 100 μ g/L as aimed concn, different amounts of DNPH is added, so that the molar ratio of DNPH and glutaraldehyde is distinguished
It is 3,6,12,24,30,60,120,180, reacts 30 minutes at room temperature, the peak area of Self -adaptive object, as a result such as Fig. 2.
The result shows that using the glutaraldehyde of 100 μ g/L as aimed concn, it, can when the molar ratio of DNPH and glutaraldehyde reaches 60
Derivatization is complete, and product peak area is not further added by substantially.
In 0 ~ 100 μ g/L concentration range when measurement, to guarantee that derivatization is complete, 2,4-dinitrophenylhydrazine and glutaraldehyde are used
The molar ratio of amount need to be greater than 60.
（3）Influence of the derivatization time to result.
The glutaraldehyde standard solution for preparing 25 μ g/L, is added same amount of DNPH, has studied the reaction time in 120 min
Influence to result, the peak area of Self -adaptive object, as a result such as Fig. 3.
The result shows that with the extension of reaction time, product peak area is gradually increased, derivatization reaction reaches after 20 min
To balance, product concentration is held essentially constant.For the applicability for guaranteeing this method, derivative 30 min of selection of time.
2, standard curve and detection limit.
（1）Standard curve.
With preparing glutaric dialdehyde standard series, upper machine testing after derivatization.The results show that glutaraldehyde has in prepared range
Preferable linear, related coefficient is greater than 0.999.
（2）Detection limit.
The glutaraldehyde standard solution of 0.50 μ g/L of compound concentration is measured in parallel 7 times, calculates standard deviation S, method detection limit
MDL=3.14*S, Determination Limit RQL=4*MDL.0.08 μ g/L of this method detection limit, 0.32 μ g/L of Determination Limit.
3, preci-sion and accuracy.
（1）Precision.
The mark-on synthesized slit into pure water prepares 1,10,50 μ g/L3 concentration respectively, replication 7 times, opposite to mark
Quasi- deviation is 1.06 ~ 4.51%.
（2）Accuracy.
Local raw water, output water are detected respectively according to the method established herein, are as a result below detection limit.
It is separately added into the standard solution of 1,10,20 μ g/L3 concentration in source water and output water, replication 7 times, adds
Marking the rate of recovery is 99.5% ~ 105.8%.
Three, conclusion.
The liquid chromatography-mass spectrography detection method for establishing glutaraldehyde in water, using 2,4-dinitrophenylhydrazine（DNPH）It is derivative
Change processing, selects InertSustainSwift（1.9μm 2.1mm×100mm）Chromatographic column, acetonitrile-pure water（Containing 2.5mmol/L
Ammonium acetate）As mobile phase, ESI negative ion mode, multiple-reaction monitoring（MRM）Mode is quantitative, direct injected, 5 μ of sample volume
L, quantified by external standard method.This method is linearly good in 0.50 ~ 70 μ g/L, and related coefficient is greater than 0.999,0.08 μ g/L of detection limit, surveys
Fix 0.32 μ g/L of limit, recovery of standard addition 99.5% ~ 105.8%.This method is simple and quick, high sensitivity, and favorable reproducibility is suitable for
The measurement of glutaraldehyde content in source water and Drinking Water.
Claims (4)
1. a kind of method of glutaraldehyde content in liquid chromatograph mass spectrography measurement water, it is characterised in that main includes following step
Suddenly：
Step 1: sample acquisition step：Obtain water sample to be measured；
Step 2: pre-treatment step：Acetonitrile and 2,4-dinitrophenylhydrazine are added in water sample to be measured, mixes immediately, reacts at room temperature
After 30 minutes, obtains derivatization sample and be fitted into sample introduction bottle after membrane filtration, it is to be measured；
Step 3: detecting step；By derivatization sample carry out liquid chromatography-mass spectrography detection, the liquid-phase condition selected for：Mobile phase
A is the pure water of the ammonium acetate containing 2.5mmol/L, and Mobile phase B is acetonitrile, chromatographic column C18, gradient elution, flow velocity 0.50mL/min,
45 DEG C of column temperature, 5 μ L of sample volume, mobile phase reference gradient is shown in Table 1：
1. mobile phase reference gradient of table：
The Mass Spectrometry Conditions selected for：Electrospray ionisation source, negative ion mode is quantitative using multiple-reaction monitoring mode, parent ion/son
Ion is 459.0/182.1 and 459.0/163.0, wherein 182.1 be quantitative daughter ion, removing cluster voltage is -90 V, collision energy
For -23.8V and -25.2V, entrance potential is -10V, and collision exit potential is -12V, -4500 V of ion spray voltage, ion source
Temperature is 500 DEG C, and gas curtain gas velocity is 20psi, and collision gas flow velocity is 6psi, and atomization gas flow velocity is 50psi, secondary air speed
For 50psi；
Step 4: calculating step：The content of glutaraldehyde in water sample to be measured is calculated.
2. the method for glutaraldehyde content, feature in a kind of liquid chromatograph mass spectrography measurement water according to claim 1
It is that water sample to be measured collected in sample acquisition step such as is tap water, then further includes to water to be measured after obtaining water sample to be measured
The step of sample dechlorination is handled, the dechlorination processing step, according to 1:1000 ratio is added 2% ascorbic acid solution and is handled.
3. the method for glutaraldehyde content, feature in a kind of liquid chromatograph mass spectrography measurement water according to claim 1
It is in sample pre-treatments step：Water sample sampling amount is 200 μ L, and acetonitrile additional amount is 700 μ L, and 2,4-dinitrophenylhydrazine concentration is
0.12mmol/L, additional amount are 100 μ L.
4. the method for glutaraldehyde content, feature in a kind of liquid chromatograph mass spectrography measurement water according to claim 1
It is in sample pre-treatments step：In 0 ~ 100 μ g/L concentration range when measurement, 2,4-dinitrophenylhydrazine and glutaraldehyde dosage
Molar ratio need to be greater than 60.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810734386.7A CN108918707A (en) | 2018-07-06 | 2018-07-06 | A kind of method that liquid chromatograph mass spectrography measures glutaraldehyde content in water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810734386.7A CN108918707A (en) | 2018-07-06 | 2018-07-06 | A kind of method that liquid chromatograph mass spectrography measures glutaraldehyde content in water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108918707A true CN108918707A (en) | 2018-11-30 |
Family
ID=64425475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810734386.7A Pending CN108918707A (en) | 2018-07-06 | 2018-07-06 | A kind of method that liquid chromatograph mass spectrography measures glutaraldehyde content in water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108918707A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113567565A (en) * | 2020-04-29 | 2021-10-29 | 江苏先声药业有限公司 | Method for detecting glutaraldehyde in amoxicillin |
| CN113740457A (en) * | 2021-09-03 | 2021-12-03 | 广东省中鼎检测技术有限公司 | Detection method for rapidly testing glutaraldehyde in consumer product |
| WO2022035990A1 (en) * | 2020-08-11 | 2022-02-17 | Saudi Arabian Oil Company | Systems and methods for the direct measurement of glutaraldehyde based biocide concentration in seawater |
| CN115219627A (en) * | 2022-07-18 | 2022-10-21 | 宁波市产品食品质量检验研究院(宁波市纤维检验所) | Method for detecting acetaldehyde content in leather |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1538169A (en) * | 2003-04-16 | 2004-10-20 | 中国科学院大连化学物理研究所 | Method of quantitative analysing glutaruldehyde by internal cabel method |
-
2018
- 2018-07-06 CN CN201810734386.7A patent/CN108918707A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1538169A (en) * | 2003-04-16 | 2004-10-20 | 中国科学院大连化学物理研究所 | Method of quantitative analysing glutaruldehyde by internal cabel method |
Non-Patent Citations (3)
| Title |
|---|
| 严坤平 等: "高效液相色谱法测定血红蛋白氧载体中残留的戊二醛", 《分析化学研究简报》 * |
| 宋新 等: "高效液相色谱法快速分析戊二醛消毒液的含量", 《河南预防医学杂志》 * |
| 王妙飞 等: "高效液相色谱法快速分析口香糖中戊二醇的残留量", 《现代食品科技》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113567565A (en) * | 2020-04-29 | 2021-10-29 | 江苏先声药业有限公司 | Method for detecting glutaraldehyde in amoxicillin |
| WO2022035990A1 (en) * | 2020-08-11 | 2022-02-17 | Saudi Arabian Oil Company | Systems and methods for the direct measurement of glutaraldehyde based biocide concentration in seawater |
| US11692979B2 (en) | 2020-08-11 | 2023-07-04 | Saudi Arabian Oil Company | Systems and methods for the direct measurement of glutaraldehyde based biocide concentration in seawater |
| CN113740457A (en) * | 2021-09-03 | 2021-12-03 | 广东省中鼎检测技术有限公司 | Detection method for rapidly testing glutaraldehyde in consumer product |
| CN115219627A (en) * | 2022-07-18 | 2022-10-21 | 宁波市产品食品质量检验研究院(宁波市纤维检验所) | Method for detecting acetaldehyde content in leather |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108918707A (en) | A kind of method that liquid chromatograph mass spectrography measures glutaraldehyde content in water | |
| Harrington et al. | Method to reduce the memory effect of mercury in the analysis of fish tissue using inductively coupled plasma mass spectrometry | |
| CN104569256B (en) | A kind of method adopting 3 kinds of OIT antiseptics in liquid chromatography tandom mass spectrometry determination water base adhesive | |
| Yang et al. | Sensitive determination of mercury by a miniaturized spectrophotometer after in situ single-drop microextraction | |
| CN104614466B (en) | The assay method of antiseptic in tobacco juice for electronic smoke | |
| CN105510480B (en) | The detection method and application of 2 methylimidazoles and 4 methylimidazoles in bakery | |
| CN106950306B (en) | Method for determining content of cysteine in compound amino acid injection | |
| CN109696499A (en) | A kind of nitrosamine Sensitive Determination method in the water based on high resolution mass spec | |
| da Rocha et al. | Speciation of mercury using capillary electrophoresis coupled to volatile species generation-inductively coupled plasma mass spectrometryPresented at the 2001 European Winter Conference on Plasma Spectrochemistry, Lillehammer, Norway, February 4–8, 2001. | |
| CN103344724A (en) | Analysis method of mancozeb | |
| CN112649552A (en) | Method for measuring selenium form by using high performance liquid inductively coupled plasma mass spectrometry | |
| CN104502468B (en) | The detection method of ethylene thiourea in plasthetics | |
| CN104502477B (en) | Organic analytical approach in a kind of trichloroacetaldehyde Waste Sulfuric Acid | |
| Kolar et al. | Chemically prepared silver electrode for determination of N-acetyl-L-cysteine by flow-injection potentiometry | |
| CN109696509B (en) | Method for detecting dimethyl sulfate residue in medicine by liquid chromatography-mass spectrometry | |
| CN108020627B (en) | Method for determining residual quantity of three phenoxy carboxylic acid pesticides in tobacco by ultra-high performance synthetic phase chromatography-tandem mass spectrometry | |
| CN106814144B (en) | Method for determining and analyzing content of dimethyl sulfate in dimethyl fumarate | |
| CN107543878A (en) | A kind of method that 3 kinds of OIT preservatives in cigarette paper are determined using Liquid Chromatography-Tandem Mass Spectrometry | |
| CN104634889B (en) | On-chip derivatization measures the method for Formaldehyde In Cosmetics | |
| CN108414460B (en) | Method for determining content of cannabidiol based on silver nano ultraviolet-visible spectrophotometry | |
| Ensafi et al. | Sensitive spectrophotometric kinetic determination of osmium by catalysis of the pyrogallol red-bromate reaction | |
| CN105424853A (en) | LC-MS/MS kit for detecting nicotine and its metabolites in saliva | |
| CN103175930B (en) | A kind of HPLC analytical method measuring sodium sulphite content | |
| CN105606740A (en) | Detection method for maleic hydrazide residual amount in tobacco and tobacco products | |
| CN110261513A (en) | The Gas Chromatographic Method of cumarin in a kind of edible essence |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181130 |