CN108918413A - A kind of multi-wavelength blood coagulation test device - Google Patents
A kind of multi-wavelength blood coagulation test device Download PDFInfo
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- CN108918413A CN108918413A CN201810756161.1A CN201810756161A CN108918413A CN 108918413 A CN108918413 A CN 108918413A CN 201810756161 A CN201810756161 A CN 201810756161A CN 108918413 A CN108918413 A CN 108918413A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/066—Modifiable path; multiple paths in one sample
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Abstract
A kind of multi-wavelength blood coagulation test device is related to in-vitro diagnosis engineering device technique field, solves the problems, such as that structure is complicated is low with the efficiency of light energy utilization for blood coagulation test device in the prior art, which includes：Multiple light sources mechanism；The reflecting mirror collecting the light beam of light source mechanism transmitting and reflecting；The dodging device of the light beam even optical transport again of reflecting mirror reflection is received, light source mechanism is around coupling assembly；Receive the light beam of dodging device transmission and the splitting optical fiber of beam splitting；For receiving the measuring mechanism of the light beam through splitting optical fiber beam splitting, measuring mechanism obtains measuring signal using received light velocity measurement determinand and retransmits to controller；The controller of light source mechanism and measuring mechanism is connected, controller control light source mechanism switch receives measuring signal and exports after handling measuring signal.The switching of wavelength light of the present invention is not necessarily to motion driving mechanism, and light beam coupling is entered dodging device and splitting optical fiber by reflective mirror, the utilization rate of luminous energy has been significantly increased, and structure is simple.
Description
Technical field
The present invention relates to in-vitro diagnosis engineering device technique fields, and in particular to a kind of multi-wavelength blood coagulation test device.
Background technique
Automatic coagulation analyzer in Vehicles Collected from Market generally uses paramagnetic particle method and optical method to detect sample, wherein optics
Method has at low cost compared to paramagnetic particle method, and the advantages such as test item is complete, are sides used by most instrument manufacturer facility quotient
Method.Wherein, optical method can be divided into transmission optics method and scattering optical method according to optic test principle.
In the patent of Publication No. CN101213437B, use halogen lamp as light source, using optical filter and rotaring wheel structure,
The monochrome of different wave length is optically coupled into splitting optical fiber, the tail end irradiation sample of splitting optical fiber measures.Due to using 5
Kind optical filter, therefore when test same project, it can be used the light of different wave length as main wave and complementary wave simultaneously, to avoid this
Influence of the bottom to a certain specific wavelength.But what the switching of its each wavelength was realized by the switching of optical filter, therefore, it is also desirable to
Corresponding motion driving mechanism, structure is complicated.
In the patent of Publication No. CN107132225A, use LED as light source, the service life of LED is much larger than Publication No.
The service life of halogen lamp in the patent of CN101213437B first collects light beam using plurality of optical fibers, then passes through optical tunnel and plural number
The fiber-optic illuminated reaction cup of root still can be because of the intrinsic duty ratio of optical fiber during collecting light beam using plurality of optical fibers, drop
The utilization rate of low luminous energy.
Therefore, the existing technology needs to be improved and developed.
Summary of the invention
In order to solve the problems, such as that structure is complicated is low with the efficiency of light energy utilization for blood coagulation test device in the prior art, the present invention is mentioned
For a kind of multi-wavelength blood coagulation test device.
Used technical solution is as follows in order to solve the technical problem by the present invention：
A kind of multi-wavelength blood coagulation test device, including multiple light sources mechanism further include：
Connect the light source mechanism and the controller for controlling light source mechanism switch；
The reflecting mirror collecting the light beam of the light source mechanism transmitting and reflecting；
The dodging device that the light beam of the reflecting mirror reflection carries out even light and transmits is received, the light source mechanism is around even light
Device setting；
Receive the light beam of the dodging device transmission and the splitting optical fiber of beam splitting；
For receiving the measuring mechanism of the light beam through the splitting optical fiber beam splitting and connection controller, the measuring mechanism is used
In obtaining measuring signal using received light velocity measurement determinand, and measuring signal is sent to controller；
The controller is for receiving measuring signal and exporting after handling measuring signal.
Further, the splitting optical fiber includes a splitting optical fiber incidence end and multiple splitting optical fiber exit ends, even light
The incident end face of device with mirror parallel and is just being correspondingly arranged, and the outgoing end face of dodging device and splitting optical fiber incidence end connect
Connect, measuring mechanism includes multiple Measurement channels, splitting optical fiber exit end by optical fiber collimator connect measuring mechanism and with measurement
Channel corresponds.
Further, the light source mechanism includes the light source set gradually, condenser, collimating mirror, optical filter and coupling mirror,
The light beam of light source transmitting is incident on collimating mirror through condenser convergence, optical filter is incident on after the collimated mirror collimation of light beam, through filtering
Piece is incident on coupling mirror after filtering, be incident on reflecting mirror after the coupled mirror coupling of light beam.
Further, the light source is semiconductor light source.
Further, the light source mechanism further includes the radiating element for connecting light source.
Further, the light source mechanism further includes the substrate for connecting light source and radiating element, is arranged in radiating element
The first temperature sensor that is interior and connecting controller and connection controller and the fan for radiating element refrigeration, the radiator
Part connects light source by substrate, and the first temperature sensor is used to detect the temperature signal of radiating element and is sent to controller, controls
Device processed is used to receive temperature signal and controls the switch and revolving speed of fan accordingly.
Further, the measuring mechanism includes reaction cup stowage receptacle, connection reaction cup stowage receptacle and is used to provide reaction
The thermostat of cup stowage receptacle institute calorific requirement, the light signal collection plate for connecting reaction cup stowage receptacle and controller, the reaction cup
Stowage receptacle includes a plurality of Measurement channel, and the quantity of Measurement channel is equal to the quantity and Measurement channel of the light beam through splitting optical fiber beam splitting
It is arranged in a one-to-one correspondence with the light beam of beam splitting, the detection of light signal collection plate is by every Measurement channel light beam to obtain measuring signal simultaneously
It is sent to controller.
Further, the thermostat includes existing positioned at the insulating layer of reaction cup stowage receptacle shell inner surface, setting
The heating device of reaction cup stowage receptacle lower surface, the thermal device for connecting reaction cup stowage receptacle and heating device, with
And the second temperature sensor in reaction cup stowage receptacle.
It further, include clamping device for clamping reaction cup in the reaction cup stowage receptacle, the reaction cup is used
In splendid attire determinand.
Further, the test device further includes：The test device further includes：Mounting base is mounted on installation bottom
The mirror unit of lens cone frame and connection lens cone frame on plate, the reflecting mirror, dodging device and light source mechanism are installed in
On mirror unit.
The beneficial effects of the invention are as follows：
1, for the switching of each wavelength light, the present invention is not necessarily to motion driving mechanism, but is controlled by controller each
The switch of a light source mechanism, light source mechanism receive any light source mechanism around dodging device setting, reflecting mirror and dodging device and pass
Defeated light beam, so that structure of the invention is simple.
2, light beam coupling is entered into dodging device and splitting optical fiber by using reflective mirror, rather than uses a plurality of optical fiber,
The problem of avoiding optical fiber duty ratio and reducing light transmissioning efficiency, has been significantly increased the utilization rate of luminous energy.
3, the structure of multi-wavelength blood coagulation test device is simplified, by using reflective mirror convenient for production and processing of the invention.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of multi-wavelength blood coagulation test device of the invention.
Fig. 2 is the structural schematic diagram of the light source mechanism in the present invention.
Fig. 3 is the structural schematic diagram of the light source mechanism and coupling mechanism in the present invention.
Fig. 4 is the structural schematic diagram of splitting optical fiber and measuring mechanism in the present invention.
Fig. 5 is the structural schematic diagram of the cross section of measuring mechanism in the present invention.
Fig. 6 is the structural schematic diagram of clamping device in the present invention.
Fig. 7 is circuit function schematic diagram of the invention.
In figure：1, light source mechanism, 1-1, light source lens barrel, 1-2, substrate, 1-3, coupling mirror, 1-4, rear spacer ring, 1-5, optical filtering
Piece, 1-6, middle spacer ring, 1-7, collimating mirror, 1-8, preceding spacer ring, 1-9, condenser, 1-10, light source, 1-11, condenser pressing ring, 1-
12, radiating element, 1-13, the first temperature sensor, 1-14, fan, 1-15, blower tray, 2, coupling mechanism are 2-1, reflective
Mirror, 2-2, lens cone frame, 2-3, mirror unit, 2-4, dodging device, 2-5, sleeve, 2-6, reflecting mirror pressing ring, 2-7, beam splitting light
Fibre, 2-71, splitting optical fiber exit end, 2-8, optical fiber collimator, 3, measuring mechanism, 3-1, reaction cup stowage receptacle, 3-11, measurement are logical
Road, 3-2, light signal collection plate, 3-21, photoelectric sensor, 3-22, primary amplifying circuit, 3-3, second temperature sensor, 3-4,
Insulating layer, 3-5, heating device, 3-6, thermal device, 3-7, reaction cup, 3-8, clamping device, 3-9, heat insulation loop, 4, control
Device processed, 4-1, fpga chip, 4-2,12 D/A chips, 4-3, light source driving circuit, 4-4, fan driving chip, 4-5,8 channels
16 A/D chips, 4-6, rear class amplifying circuit, 5, mounting base.
Specific embodiment
To better understand the objects, features and advantages of the present invention, with reference to the accompanying drawing and specific real
Applying mode, the present invention is further described in detail.
In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention, still, the present invention may be used also
To be implemented using other than the one described here other modes, therefore, protection scope of the present invention is not by described below
Specific embodiment limitation.
The description for being such as related to " first ", " second " in the present invention is used for description purposes only, and should not be understood as indicating
Or it implies its relative importance or implicitly indicates the quantity of indicated technical characteristic.
Please refer to Fig. 1-Fig. 7, the present invention provides a kind of multi-wavelength blood coagulation test device, such as Fig. 1, including：Transmitting
The light source mechanism 1 of light beam, the light beam for collecting the sending of light source mechanism 1 simultaneously irradiate the coupling mechanism 2 of determinand, measuring mechanism 3
And controller 4.Coupling mechanism 2 includes reflecting mirror 2-1, dodging device 2-4 and splitting optical fiber 2-7, and reflecting mirror 2-1 collects light source
The light beam and the reflected beams that mechanism 1 emits, dodging device 2-4 receive the light beam of reflecting mirror 2-1 reflection, carry out even light, then pass
Splitting optical fiber 2-7 is transported to, splitting optical fiber 2-7 receives the light beam of dodging device 2-4 transmission and beam splitting, is divided into several beams.Light source machine
The quantity of structure 1 be it is multiple, light source mechanism 1 is looped around outside dodging device 2-4, reflecting mirror 2-1 can receive any one/multiple light sources machine
The light beam that structure 1 transmits.Light source mechanism 1 and measuring mechanism 3 are connect with controller 4.Splitting optical fiber 2-7 is connect with measuring mechanism 3.
Measuring mechanism 3 is also used to utilize received light beam (beam splitting light for receiving the light beam through splitting optical fiber 2-7 beam splitting, measuring mechanism 3
The light beam of fine 2-7 beam splitting) measurement determinand obtain measuring signal, measuring signal be sent to controller 4.Controller is for receiving
The measuring signal that measuring mechanism 3 is sent, and exported after handling measuring signal.Controller 4 is also used to control light source mechanism 1
Switch.Light source mechanism 1 emits light beam, and reflecting mirror 2-1 receives the light beam that coupling light source mechanism 1 emits, and beam Propagation to even light fills
The even light of 2-4 is set, then is transmitted to splitting optical fiber 2-7, splitting optical fiber 2-7 is split light beam, and the light beam of beam splitting irradiates measuring machine
Structure 3, measuring mechanism 3 receive the light of splitting optical fiber 2-7 irradiation, are tested, obtain measuring signal and be sent to controller 4, control
Device 4 exports after handling measuring signal.
Switching for each wavelength light, the present invention is not necessarily to motion driving mechanism, but is controlled by controller 4 each
The switch of light source mechanism 1, light source mechanism 1 are arranged around dodging device 2-4, reflective mirror 2-1 and dodging device 2-4 receive any light
The light beam that source mechanism 1 transmits, so that structure of the invention is simple.The light beam for being issued light source mechanism 1 by using reflective mirror 2-1
It is coupled into dodging device 2-4, rather than uses a plurality of optical fiber or splitting optical fiber, the utilization of luminous energy has been significantly increased
Rate.In addition, simplifying the structure of multi-wavelength blood coagulation test device, by using reflective mirror 2-1 convenient for production and processing of the invention.
Reflecting mirror 2-1, dodging device 2-4 and splitting optical fiber 2-7 are arranged according to optical path, reflecting mirror 2-1 and multiple light sources machine
The optical axis of structure 1 is in certain fixed angle, and the reflecting surface of reflecting mirror 2-1 is parallel with the incident end face of dodging device 2-4.Preferably
Splitting optical fiber 2-7 is close to around dodging device 2-4 arranged at equal intervals, the outgoing end face of dodging device 2-4 in multiple light sources mechanism 1
Incidence end.
Specifically, multiple light sources mechanism 1 can launch the monochromatic light of different wave length, the light that each light source mechanism 1 issues
Wavelength is typically different, but each light source mechanism 1 usually only emits a kind of monochromatic light of wavelength, the machinery of multiple light sources mechanism 1
Size can be all the same.The incident end face of dodging device 2-4 is parallel with reflecting mirror 2-1 and is just correspondingly arranged.Light source mechanism 1 emits
With the light beam of 1 optical axis coincidence of light source mechanism after reflecting mirror 2-1 reflection, be just irradiated to the center of dodging device 2-4, because
This each light source mechanism 1 is interchangeable, when not needing some wavelength, can dismantle this light source mechanism 1.If the light of some wavelength
Energy is weaker, can install the light source mechanism 1 of this wavelength again.The light beam of these light source mechanisms 1 transmitting is successively through reflecting mirror 2-1
Reflection, the even light of dodging device 2-4, splitting optical fiber 2-7 import the light beam of these different wave lengths in measuring mechanism 3.Measuring mechanism 3
The light beam of these different wave lengths of irradiation is detected, measuring mechanism 3 obtains measuring signal, and measuring signal is transferred to controller 4,
Controller 4 externally exports after handling measuring signal, and signal conversion process is that measuring mechanism 3 detects optical signal and is converted to electricity
Signal is sent to controller 4, and controller 4 converts electrical signals to digital signal output, and wherein electric signal is that above-mentioned measurement is believed
Number.If a kind of multi-wavelength blood coagulation test device of the present invention is directly installed on blood coagulation analyzer, the controller 4 can will be digital
Signal is sent to the main control board in blood coagulation analyzer, or with the matching used computer of blood coagulation analyzer.
In embodiment of the present invention, as shown in Fig. 2, light source mechanism 1 includes：Light source lens barrel 1-1, substrate 1-2, coupling mirror 1-
3, rear spacer ring 1-4, optical filter 1-5, middle spacer ring 1-6, collimating mirror 1-7, preceding spacer ring 1-8, condenser 1-9, condenser pressing ring 1-11,
Light source 1-10, radiating element 1-12 and the first temperature sensor 1-13.Each light source mechanism 1 is made of above-mentioned component, due to
The wavelength of light source 1-10 used is different, and coupling mirror 1-3, collimating mirror 1-7 and the surface condenser 1-9 institute's film plating layer and light source 1-10 are sent out
Out Wavelength matched, to improve the transmitance of light beam.
Light source 1-10 and condenser pressing ring 1-11 is arranged in light source lens barrel 1-1 on the left side of substrate 1-2, substrate 1-2, from the right side
To a left side set gradually condenser pressing ring 1-11, condenser 1-9, preceding spacer ring 1-8, collimating mirror 1-7, middle spacer ring 1-6, optical filter 1-5,
Spacer ring 1-4 and coupling mirror 1-3 afterwards, coupling mirror 1-3, rear spacer ring 1-4, optical filter 1-5, middle spacer ring 1-6, collimating mirror 1-7, preceding spacer ring
1-8, condenser 1-9, condenser pressing ring 1-11 and light source 1-10 are respectively positioned in light source lens barrel 1-1, coupling mirror 1-3, rear spacer ring 1-
4, optical filter 1-5, middle spacer ring 1-6, preceding spacer ring 1-8 and condenser pressing ring 1-11 are all connected with light source lens barrel 1-1, condenser pressing ring 1-
11 fixed condenser 1-9.Every kind of light emitting diode is high power light emitting diode, and calorific value is larger, therefore, by above-mentioned five
The light source 1-10 of kind different wave length is welded on substrate 1-2, and substrate 1-2 makes (such as aluminium base using the higher material of thermal coefficient
Plate), in this way, the heat transfer that light source 1-10 is generated when working gives substrate 1-2.Substrate 1-2 is tightly bonded with radiating element 1-12,
Gap between radiating element 1-12 and substrate 1-2 can be filled with heat-conducting silicone grease, and is mounted with inside radiating element 1-12
One temperature sensor 1-13 the first temperature sensor 1-13 and dissipates in order to quickly detect the temperature of radiating element 1-12
Gap between thermal device 1-12 is equally filled with heat-conducting silicone grease.
Specifically, multiple light sources mechanism 1 is usually arranged as five, and the optical filter 1-5 in embodiment selects narrowband to filter
Mating plate, half-wave bandwidth are 8~12nm, and cut-off depth is greater than 4, therefore, the different waves of used five kind in embodiment of the present invention
Long light source 1-10 is either wave band and the matched monochromatic source of above-mentioned five kinds of optical filter 1-5, are also possible to comprising above-mentioned five
The broadband secondary color light source of kind optical filter 1-5 central wavelength.In embodiment of the present invention, light source 1-10 is semiconductor light source,
Such as light emitting diode, it can also be other semiconductor light sources certainly.Now using one of light emitting diode as light source 1-10
And its corresponding optical path is illustrated, and the light emitting anger of light emitting diode is bigger, and about 120 °, condenser 1-9 adjacent thereto
The light issued to light emitting diode is converged and is collected, what the collimating mirror 1-7 at the rear condenser 1-9 collected condenser 1-9
It after light beam is collimated, injects optical filter 1-5 and filters, then penetrate light beam after the coupling mirror 1-3 at the rear optical filter 1-5 coupling
Out, that is, the transmitting of light source mechanism 1 is completed, light beam is incident on reflective mirror 2-1.Since the half-wave bandwidth of optical filter 1-5 is narrow, together
When provide that the angle of its incident light is not greater than its specified incident angle.The half-wave of optical filter 1-5 in embodiment of the present invention
Bandwidth is 8~12nm, and incident angle is ± 7 °,
Since the light stability of light emitting diode is greatly influenced by temperature, therefore, it is necessary to the first temperature sensor 1-13
Precision it is unsuitable too low, at least should be less than 0.5 DEG C, the first temperature sensor 1-13 selected in the present invention is platinum resistance, essence
Degree is 0.3 DEG C, and the first temperature sensor 1-13 connection controller 4, the first temperature sensor 1-13 is by the radiating element 1- of acquisition
The controller 4 that 12 temperature feedback is given.
Multiple light sources 1-10 is at least three：Including first light source, second light source and third light source, first light source hair
Freezing method tests the light beam to be tested of wavelength used out, and second light source issues the light to be tested that Chromogenic assay tests wavelength used
Beam, third light source issue the light beam to be tested that immunoturbidimetry tests wavelength used.Specifically, first light source shines for 660nm
Diode, second light source are 405nm light emitting diode, and third light source is 575nm light emitting diode.
Further, sample is human plasma, generally by human whole blood and sodium citrate according to 9：1 volume example mixing,
Prepared using centrifugation, at this point, the composition of the main coagulation factor in blood plasma be factor I, II, V, VII, VIII,
IX, X, XI, XII, XIII；Anticoagulant composition is mainly Antithrombin III；(fibrin degradation produces the product FDP of fibrinolytic system
Object) and d-dimer.
When freezing method reagent is added, after sample is mixed with reagent, final product is the fibrin for being crosslinked shape, herein
In reaction process, when mixture of the light beam through sample and reagent, show as that transmitted light is more and more weaker, and scattering light is increasingly
By force, when test sample freezing method project, it is contemplated that the influence that sample background generates, dominant wavelength 660nm, complementary wave are a length of
800nm。
When Chromogenic assay reagent is added, after sample is mixed with reagent, Antithrombin III inhibits the part in reagent solidifying
Hemase activity, remaining blood coagulation enzyme-to-substrate start to react, and product is p-NA (p-nitrophenol), are in yellow.It reacted herein
The mixture of Cheng Zhong, sample and reagent is increasing to the absorbance of specific wavelength light beam (such as 405nm or other ultraviolet bands),
And absorbance and concentration are directly proportional, that is, meet Lambert-Beer's law, dominant wavelength 405nm, a length of 340nm of complementary wave.
When immunoturbidimetry reagent is added, after sample is mixed with reagent, antigen leads to the glue in solution in conjunction with antibody
Newborn particle is assembled, and in this reaction process, when light beam is through the mixture of sample and reagent, shows as transmitted light and gets over
Come it is weaker, when test sample immunoturbidimetry project, it is contemplated that sample background generate influence, dominant wavelength 575nm, complementary wave
A length of 800nm.
As shown in figure 3, coupling mechanism 2 includes reflective mirror 2-1, dodging device 2-4 and splitting optical fiber 2-7.
Dodging device 2-4, reflecting mirror 2-1 and light source mechanism 1 are installed in mirror unit 2-3.Mirror unit 2-3 and peace
Dress bottom plate 5 is connected and is fixed by lens cone frame 2-2.Reflecting mirror 2-1 is screwed in by way of screw thread in mirror unit 2-3, and
It is adjusted at a distance from dodging device 2-4 incident end face, reflecting mirror pressing ring 2-6 screws in mirror unit 2- also by the mode of screw thread
In 3, and compress reflecting mirror 2-1.In the incident end face insertion mirror unit 2-3 of dodging device 2-4, meanwhile, outside dodging device 2-4
The sleeve 2-5 of side is threaded into mirror unit 2-3, and fixed dodging device 2-4.Splitting optical fiber 2-7
Including a splitting optical fiber incidence end and multiple splitting optical fiber exit end 2-71.The incident end face face of dodging device 2-4 reflects
The outgoing end face of mirror 2-1, dodging device 2-4 and splitting optical fiber incidence end in contact.Splitting optical fiber exit end 2-71 passes through optical fiber standard
Straight device 2-8 is connected with measuring mechanism 3, and multiple splitting optical fiber exit end 2-71 and multiple Measurement channel 3-11 mono- hereafter are a pair of
It answers, optical fiber collimator 2-8 can converge the splitting optical fiber exit end 2-71 light projected, and irradiate in a substantially parallel manner
To measuring mechanism 3.
Light beam is projected the reflecting mirror 2-1 of irradiation in its rear by coupling mirror 1-3, and reflecting mirror 2-1 issues light source mechanism 1
Light reflects and enters the incident end face of dodging device 2-4, is greater than even light in the spot size that dodging device 2-4 incident end face is formed
Device 2-4 incidence end after light beam carries out multiple total reflection in dodging device 2-4, is penetrated from the outgoing end face of dodging device 2-4
Out, the total reflection number occurred in dodging device 2-4 is more, and the energy of the light projected from the outgoing end face of dodging device 2-4 is got over
Uniformly, the cross section of the dodging device 2-4 in apparatus of the present invention is hexagon.
In embodiment of the present invention, reflecting mirror 2-1 at a distance from the incident end face of dodging device 2-4 be adjusted, guarantee with
The light beam of 1 optical axis coincidence of light source mechanism is reflected into the center of dodging device 2-4 incident end face, reflecting mirror 2-1 by reflecting mirror 12d
The reflecting mirror pressing ring 2-6 stationary mirror 2-1 at rear.
Light source mechanism 1 matches fan 1-14 and blower tray 1-15 in Fig. 3, and fan 1-14 and blower tray 1-15 connect
It connects, blower tray 1-15 is connect with mounting base 5.Radiating element 1-12 is freezed by fan 1-14, fan 1-14 refrigeration
The revolving speed of itself can also be detected and adjust simultaneously, fan 1-14 is controlled by controller 4, controls its switch and revolving speed, control
Device 4 processed adjusts the operation of fan 1-14 according to the temperature of the first temperature sensor 1-13 radiating element 1-12 fed back in real time and turns
Speed.Namely the radiating mode of substrate 1-2 is the form that fan 1-14 is combined with radiating element 1-12, but is not limited only to such shape
Formula, it is possible to use semiconductor chilling plate replaces radiating element 1-12.
As shown in Figure 4 and Figure 5, measuring mechanism 3 includes thermostat, light signal collection plate 3-2 and reaction cup to measuring mechanism 3
Stowage receptacle 3-1, thermostat include insulating layer 3-4, heating device 3-5, thermal device 3-6 and second temperature sensor 3-
3.Measuring mechanism further includes reaction cup stowage receptacle 3-1 and multiple reaction cup 3-7 for containing determinand.Reaction cup loads
There is Measurement channel 3-11 in seat 3-1, it can placing response cup 3-7 in Measurement channel 3-11.It is any one in Measurement channel 3-11
Item can be used as the Measurement channel 3-11 of reference, here as reference Measurement channel 3-11 only one, as reference
Measurement channel 3-11 be not put into reaction cup 3-7 when the invention works, remaining Measurement channel 3-11 is put into reaction cup when work
3-7.Second temperature sensor 3-3 is inserted into the inside of reaction cup stowage receptacle 3-1, for detecting and controlling reaction cup stowage receptacle 3-1
Internal temperature.
The shell inner surface of reaction cup stowage receptacle 3-1 is insulating layer 3-4, prevents heat losses into air, reduces air
Flow the influence generated to reaction cup stowage receptacle 3-1.(outer lower face) pastes heating device 3- below reaction cup stowage receptacle 3-1
5, heating device 3-5 can quickly transfer heat to reaction cup stowage receptacle 3-1.Thermal device 3-6 and reaction cup stowage receptacle
3-1 and heating device 3-5 is close to, and prevents second temperature sensor 3-3 from damaging and reaction cup stowage receptacle 3-1 is caused overheat occur now
As.Reaction cup stowage receptacle 3-1 is separated with mounting base 5 by heat insulation loop 3-9, prevents the heat transfer of reaction cup stowage receptacle 3-1 extremely
Bottom plate 1a.Light signal collection plate 3-2 includes multiple photoelectric sensor 3-21, multiple photoelectric sensor 3-21 and reaction cup stowage receptacle
Measurement channel 3-11 in 3-1 connection, photoelectric sensor 3-21 and reaction cup stowage receptacle 3-1 is corresponded.Each measurement in Fig. 4
The rear of channel 3-11, and that direction and optical fiber collimator 2-8 face is photoelectric sensor 3-21.Reaction cup stowage receptacle in Fig. 4
The quantity of 3-1 is 2, and corresponding light signal collection plate 3-2 is also 2.Light signal collection plate 3-2 connection controller 4.Measuring machine
Optical signal is become electric signal by the light signal collection plate 3-2 in structure 3, and electric signal is transferred to the controller 4, Mei Geguang of connection
The information of electric transducer 3-21 detection is transmitted to controller 4, and using electric signal as measuring signal, controller 4 becomes electric signal
It is externally exported for digital signal.In embodiment of the present invention, two light signal collection plate 3-2 can be identical, and optical signal is adopted
Collecting plate 3-2 includes multiple photoelectric sensor 3-21 and multiple primary amplifying circuit 3-22.
Optical fiber collimator 2-8 the splitting optical fiber exit end 2-71 light projected converge and in a substantially parallel manner according to
It is mapped in measuring mechanism 3, is specially irradiated in the Measurement channel 3-11 of reaction cup stowage receptacle 3-1.Sample and reagent are placed on instead
After answering in glass 3-7, reaction cup 3-7 is put into Measurement channel 3-11, and the directional light irradiation that splitting optical fiber exit end 2-71 is projected is anti-
The mixture of the sample and reagent in glass 3-7 is answered, transmitted light is received by photoelectric sensor 3-21.Measurement channel 3- as reference
11 are not put into reaction cup 3-7 at work, but still record optical signal, remaining Measurement channel 3-11 records sample and reagent reaction
When optical signal, due to these signals can pass through light signal collection plate 3-2 and controller 4, inevitably bring interference signal,
At this point, can will be done as the Measurement channel 3-11 optical signal collected of reference and remaining Measurement channel 3-11 optical signal acquired
Difference removes interference signal.
The material of reaction cup stowage receptacle 3-1 is aluminium alloy, when reaction cup 3-7 intercalation reaction cup stowage receptacle 3-1, by air
It bathes and the reactant of sample and reagent in reaction cup 3-7 is heated, in embodiment of the present invention, the wall of cup of reaction cup 3-7 and anti-
The distance of a glass stowage receptacle 3-1 instrument connection (reaction cup stowage receptacle 3-1 instrument connection is used for placing response cup 3-7) inner wall is answered to be less than
0.1mm.Reaction cup stowage receptacle 3-1 instrument connection is located in Measurement channel 3-11.
It is clamping device 3-8 above reaction cup stowage receptacle 3-1 instrument connection, as shown in fig. 6, it is fixed on the top of instrument connection,
The clamping device 3-8 selected in embodiment of the present invention is extension spring, is the cross section of clamping device 3-8 shown in Fig. 6, when
When reaction cup 3-7 is put into the instrument connection in reaction cup stowage receptacle 3-1, clamping device 3-8 is squeezed, generates deformation, right at this time
Reaction cup 3-7 generates power F to the left, to make the top edge of instrument connection of the reaction cup 3-7 in reaction cup stowage receptacle 3-1.
Such as Fig. 7, controller includes fpga chip 4-1,12 D/A chip 4-2, light source driving circuit 4-3, fan driving core
Piece 4-4,16,8 channel A/D chip 4-5 and rear class amplifying circuit 4-6.Present embodiment invention has 20 photoelectric sensor 3-
21, each photoelectric sensor 3-21 are attached to a primary amplifying circuit 3-22, and photoelectric sensor 3-21 with its follow just
Grade amplifying circuit 3-22 is welded on light signal collection plate 3-2, and electric signal is transferred to control by each primary amplifying circuit 3-22
Rear class amplifying circuit 4-6 in device 4, in light signal collection plate 3-2, the primary amplification that each photoelectric sensor 3-21 is followed is electric
The gain of road 3-22 is all the same, and still, the gain of rear class amplifying circuit 4-6 is transformed into different increasings with the switching of wavelength
Benefit.Amplified 20 road electric signal is sent to 16,38 channels A/D chip 4-5, electric signal conversion by rear class amplifying circuit 4-6
It is sent to fpga chip 4-1 for digital signal, digital signal can be sent to the master control borad of blood coagulation analyzer by fpga chip 4-1
Or host computer.
Another function of fpga chip 4-1 is to drive the light source 1-10 on-off of five kinds of different wave lengths, and fpga chip 4-1 connects
It meets 5 12 D/A chip 4-2 and 5 light source driving circuit 4-3, fpga chip 4-1 and digital signal is sent to 12 D/A cores
Piece 4-2, this 12 D/A chip 4-2 convert digital signals into analog signal and are sent to light source driving circuit 4-3, in the present invention
Light source driving circuit 4-3 be constant current source chip.The driving current value and on off operating mode of each light source can be independently arranged, FPGA
Chip 4-1 connection the first temperature sensor 1-13, and acquire the temperature data of the first temperature sensor 1-13, fpga chip 4-1
By connecting fan driving chip 4-4 driving fan 1-14.340nm light source, 405nm light source, 575nm light source in Fig. 7,
That 660nm light source and 800nm light source indicate is the light source 1-10 of five light source mechanisms 1 of present embodiment.
The present invention is that the light source of five kinds of different wave lengths is passed through the monochromatic light that corresponding optical path is launched, and passes through reflection
Mirror 2-1 is reflected into dodging device 2-4, is entered back into splitting optical fiber 2-7 after even light, and splitting optical fiber exit end 2-71 is by five kinds
The monochromatic light exposure reaction cup 3-7 of different wave length, since each instrument connection of measuring mechanism 3 is within a respective testing time
A project can only be tested, and this test item needs the light of a kind of wavelength or another wavelength, to avoid different wave length
Influence each other between monochromatic light, then the monochromatic light of five kinds of different wave lengths must time-division illumination, i.e., in a certain order and frequency
Successively light on and off, i.e., in one cycle, there are five types of the samples and reagent in the monochromatic light time-division illumination reaction cup 3-7 of different wave length
Mixture, photoelectric sensor 3-21 acquires the monochromatic optical signal of five kinds of different wave lengths simultaneously, and by the optical signal of five kinds of wavelength
The electric signal for amplifying and being converted to 16 A/D chip 4-5 of electric signal and 8 channels is converted to digital signal, is sent to FPGA
Chip 4-1, finally by fpga chip 4-1 by the digital signal of 5 kinds of different wave lengths be sent to blood coagulation analyzer master control borad or on
Position machine, master control borad or host computer are analyzed according to signal needed for test item selection, in this way, each survey can be achieved in the present invention
It measures channel 3-11 and detects any test item.
Claims (10)
1. a kind of multi-wavelength blood coagulation test device, including multiple light sources mechanism (1), which is characterized in that further include：
Connect the light source mechanism (1) and the controller (4) for controlling light source mechanism (1) switch；
The reflecting mirror (2-1) collecting the light beam of the light source mechanism (1) transmitting and reflecting；
Receive the dodging device (2-4) that the light beam of the reflecting mirror (2-1) reflection carries out even light and transmits, the light source mechanism
(1) it is arranged around dodging device (2-4)；
Receive the light beam of the dodging device (2-4) transmission and the splitting optical fiber (2-7) of beam splitting；
For receiving the measuring mechanism (3) of the light beam through the splitting optical fiber (2-7) beam splitting and connection controller (4), the survey
Measuring mechanism (3) is used to obtain measuring signal using received light velocity measurement determinand, and measuring signal is sent to controller
(4)；
The controller (4) is for receiving measuring signal and exporting after handling measuring signal.
2. a kind of multi-wavelength blood coagulation test device as described in claim 1, which is characterized in that splitting optical fiber (2-7) packet
Include a splitting optical fiber incidence end and multiple splitting optical fiber exit ends (2-71), the incident end face of dodging device (2-4) and reflection
Mirror (2-1) is parallel and is just correspondingly arranged, and the outgoing end face of dodging device (2-4) is connect with splitting optical fiber incidence end, measuring mechanism
It (3) include multiple Measurement channels (3-11), splitting optical fiber exit end (2-71) connects measuring mechanism by optical fiber collimator (2-8)
(3) and with Measurement channel (3-11) it corresponds.
3. a kind of multi-wavelength blood coagulation test device as described in claim 1, which is characterized in that the light source mechanism (1) includes
Light source (1-10), condenser (1-19), collimating mirror (1-7), optical filter (1-5) and the coupling mirror (1-3) set gradually, light source
The light beam of (1-10) transmitting is incident on collimating mirror (1-7) after condenser (1-19) convergence, after the collimated mirror of light beam (1-7) collimation
It is incident on optical filter (1-5), is incident on coupling mirror (1-3) after optical filter (1-5) optical filtering, the coupled mirror of light beam (1-3) coupling
After be incident on reflecting mirror (2-1).
4. a kind of multi-wavelength blood coagulation test device as claimed in claim 3, which is characterized in that the light source (1-10) is partly to lead
Body light source.
5. a kind of multi-wavelength blood coagulation test device as claimed in claim 3, which is characterized in that the light source mechanism (1) is also wrapped
Include the radiating element (1-12) of connection light source (1-10).
6. a kind of multi-wavelength blood coagulation test device as claimed in claim 5, which is characterized in that the light source mechanism (1) is also wrapped
The substrate (1-2) of connection light source (1-10) and radiating element (1-12) is included, is arranged in radiating element (1-12) and connection controls
The first temperature sensor (1-13) of device (4) and connection controller (4) and the fan (1- for radiating element (1-12) refrigeration
14), the radiating element (1-12) is by substrate (1-2) connection light source (1-10), and the first temperature sensor (1-13) is for visiting
It surveys the temperature signal of radiating element (1-12) and is sent to controller (4), controller (4) is for receiving temperature signal and controlling accordingly
The switch and revolving speed of fan (1-14) processed.
7. a kind of multi-wavelength blood coagulation test device as described in claim 1, which is characterized in that the measuring mechanism (3) includes
Reaction cup stowage receptacle (3-1) connects reaction cup stowage receptacle (3-1) and for providing reaction cup stowage receptacle (3-1) institute calorific requirement
Thermostat, the light signal collection plate (3-2) for connecting reaction cup stowage receptacle (3-1) and controller (4), the reaction cup stowage receptacle
(3-1) includes a plurality of Measurement channel (3-11), and the quantity of Measurement channel (3-11) is equal to the light beam through splitting optical fiber (2-7) beam splitting
Quantity and Measurement channel (3-11) and the light beam of beam splitting be arranged in a one-to-one correspondence, light signal collection plate (3-2) detection pass through every
Measurement channel (3-11) light beam is to obtain measuring signal and be sent to controller (4).
8. a kind of multi-wavelength blood coagulation test device as claimed in claim 7, which is characterized in that the thermostat includes being located at
The insulating layer (3-4) of reaction cup stowage receptacle (3-1) shell inner surface is arranged in reaction cup stowage receptacle (3-1) outer lower face
Heating device (3-5), the thermal device (3-6) for connecting reaction cup stowage receptacle (3-1) and heating device (3-5), with
And it is located at the second temperature sensor (3-3) in reaction cup stowage receptacle (3-1).
9. a kind of multi-wavelength blood coagulation test device as described in claim 1, which is characterized in that the reaction cup stowage receptacle (3-
It 1) include for clamping the clamping device of reaction cup (3-7) (3-8), the reaction cup (3-7) is for containing determinand.
10. a kind of multi-wavelength blood coagulation test device as described in claim 1, which is characterized in that the test device further includes：
Mounting base (5), the lens cone frame (2-2) being mounted on mounting base (5) and the mirror unit for connecting lens cone frame (2-2)
(2-3), the reflecting mirror (2-1), dodging device (2-4) and light source mechanism (1) are installed on mirror unit (2-3).
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| CN201810756161.1A CN108918413A (en) | 2018-07-11 | 2018-07-11 | A kind of multi-wavelength blood coagulation test device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810756161.1A CN108918413A (en) | 2018-07-11 | 2018-07-11 | A kind of multi-wavelength blood coagulation test device |
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| CN108918413A true CN108918413A (en) | 2018-11-30 |
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| CN109709345A (en) * | 2018-12-29 | 2019-05-03 | 迪瑞医疗科技股份有限公司 | One kind two is to beam splitting type blood coagulation test device |
| CN113994209A (en) * | 2019-07-31 | 2022-01-28 | 深圳迈瑞生物医疗电子股份有限公司 | Sample detection module and sample analyzer |
| WO2022059524A1 (en) * | 2020-09-16 | 2022-03-24 | コニカミノルタ株式会社 | Photometer |
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Application publication date: 20181130 |