CN108918239A - A kind of preparation method of blood separation sensing membrane - Google Patents
A kind of preparation method of blood separation sensing membrane Download PDFInfo
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- CN108918239A CN108918239A CN201810672975.7A CN201810672975A CN108918239A CN 108918239 A CN108918239 A CN 108918239A CN 201810672975 A CN201810672975 A CN 201810672975A CN 108918239 A CN108918239 A CN 108918239A
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- preparation
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- conducting polymer
- reaction
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- 239000008280 blood Substances 0.000 title claims abstract description 38
- 210000004369 blood Anatomy 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 239000012528 membrane Substances 0.000 title claims abstract description 25
- 238000000926 separation method Methods 0.000 title claims abstract description 22
- 238000006243 chemical reaction Methods 0.000 claims abstract description 23
- 239000002322 conducting polymer Substances 0.000 claims abstract description 22
- 229920001940 conductive polymer Polymers 0.000 claims abstract description 22
- 239000000178 monomer Substances 0.000 claims abstract description 21
- 150000001450 anions Chemical class 0.000 claims abstract description 11
- 150000001768 cations Chemical class 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims description 7
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims description 6
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Thiophene Chemical compound C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 claims description 6
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 5
- 235000012489 doughnuts Nutrition 0.000 claims description 4
- 229910021592 Copper(II) chloride Inorganic materials 0.000 claims description 3
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 229930192474 thiophene Natural products 0.000 claims description 3
- 238000003828 vacuum filtration Methods 0.000 claims description 3
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 2
- 150000003233 pyrroles Chemical class 0.000 claims description 2
- 230000008021 deposition Effects 0.000 claims 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 34
- 238000000034 method Methods 0.000 description 11
- 238000001514 detection method Methods 0.000 description 9
- 239000000463 material Substances 0.000 description 7
- 102100026189 Beta-galactosidase Human genes 0.000 description 6
- 108010059881 Lactase Proteins 0.000 description 6
- 108010005774 beta-Galactosidase Proteins 0.000 description 6
- 229940116108 lactase Drugs 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- 239000011540 sensing material Substances 0.000 description 4
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 3
- 229910052804 chromium Inorganic materials 0.000 description 3
- 239000011651 chromium Substances 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000012742 biochemical analysis Methods 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 229920001661 Chitosan Polymers 0.000 description 1
- 239000004695 Polyether sulfone Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910000510 noble metal Inorganic materials 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Abstract
The present invention provides a kind of novel processing steps of blood separation sensing membrane, including：Prepare conducting polymer monomer solution；Prepare the water solution A containing reaction anion and reaction cation；Supporter is immersed in solution A and is filtered by vacuum, enters reaction solution in supporter duct；Then supporter is taken out, then is immersed in conducting polymer monomer solution, certain time taking-up is stood, it is dry, complete the preparation of film.Preparation method provided by the invention is easy to operate, low manufacture cost, has good large-scale production prospect.
Description
Technical field
The present invention relates to a kind of novel method for preparing blood separation sensing membrane, quick separating and progress for blood
In situ detection.
Background technique
The blood assay techniques of modern medical service depend on two processes of centrifugation extraction and biochemical analysis of blood mostly.More than
Process takes a long time and can not obtain testing result in situ.At present for this problem have research method propose by blood separation with
Two processes of biochemical analysis combine, and fusion membrane separation technique and biosensor technique construct a kind of blood and separate sensing membrane.
The membrane material of existing medical seperation film mainly includes polysulfones, polyether sulfone, cellulose, chitosan, polyacrylamide
Equal high-molecular organic materials.Above-mentioned material all has certain blood compatibility and separating effect, but cannot achieve weight in blood
Want the detection of index.And being usually used in biological sensing material includes noble metal, inorganic builder or combination materials.The above material has good
Good conductive catalytic effect, but often blood compatibility is poor and is not easy to form a film.Therefore, in the preparation of blood separation sensing membrane
In, how key is by the conductive catalytic activity phase of the film forming of organic film material, blood compatibility and inorganic sensing material
In conjunction with.Traditional organic film differs greatly with the preparation method of inorganic sensing material, is not available a kind of method realization organic film
Material is merged with inorganic sensing material.Therefore, suitable material is found, novel preparation method is designed, a step constructs blood
Sensing membrane is separated, is had great importance for shortening the Blood diagnosis time, improving clinical treatment efficiency.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation methods of novel blood separation sensing membrane, simplify film preparation stream
Journey reduces cost.
The technical scheme is that：A kind of preparation method of blood separation sensing membrane, specific step is as follows：
(1) preparation of reaction solution：Prepare conducting polymer monomer solution；Prepare containing reaction anion and reaction sun from
The water solution A of son；Wherein the conducting polymer monomer solution is one of pyrroles, thiophene or aniline solution, reaction yin
Ion is K3Fe(CN)6、KAu(CN)4Or K3Co(CN)6One of solution, the reaction cation is FeCl3、CuCl2Or
NiCl2One kind of solution；
(2) on doughnut supporter film preparation：Doughnut supporter one end is sealed, the other end connects vacuum
Pump；Supporter is immersed in solution A, vacuum filtration takes out after a certain period of time；Supporter is submerged into conducting polymer list again
In body aqueous solution, certain time taking-up is stood, the preparation process of film is completed, and it is dry, obtain blood separation sensing membrane.
The concentration of conducting polymer monomer solution described in preferred steps (1) is 0.01-0.1M.
The concentration that anion is reacted described in preferred steps (1) is 0.001-0.01M, and the concentration for reacting cation is
0.001-0.01M。
Environment temperature in preferred steps (2) is controlled at 25-60 DEG C；Vacuum pressure is 0.05-0.1MPa；The pumping of solution A
The filter time is 1-5min；The Immersion time of conducting polymer monomer solution is 1-3h；Dry is naturally dry or drying, drying
Temperature range is 40-60 DEG C.
It is preferred that blood separation sensing membrane with a thickness of 100-500nm.
Beneficial effect：
The present invention proposes a kind of novel processing step of blood separation sensing membrane.This method makes conducting polymer monomer and yin
Cationic step copolymerization, which is deposited in supporter duct, forms separation and sensing film layer.It is dense by control coagulation time, reaction solution
The conditions such as degree adjust membrane aperture, to achieve the purpose that retain haemocyte.The desired physiological substance in blood is sensed simultaneously
Detection.This method further simplifies the preparation flow of blood separation sensing membrane, and the film of preparation reaches the rejection of haemocyte
100%, 134.6mA M is up to the detection sensitivity of blood lactase acid-1。
Detailed description of the invention
Fig. 1 is a kind of profile scanning electron microscope of blood separation sensing membrane prepared by embodiment 1.
Specific embodiment
Embodiment 1
(1) chromium solution is prepared, prepares and contains K3Fe(CN)6And FeCl3The solion of solution, conducting polymer monomer water
Solution concentration is 0.1M, and reaction anion concentration is 0.01M, and reaction cation concn is 0.001M.
(2) supporter one end is sealed, the other end connects vacuum pump, and supporter is immersed in by environment temperature control at 35 DEG C
In solution A, taken out after 1min is filtered by vacuum, vacuum pressure 0.1MPa；Supporter is immersed in conducting polymer monomer water again
It is taken out after 1h in solution.The film naturally dry that will be prepared, film thickness reach 300nm.Profile scanning electron microscope such as Fig. 1 of prepared film
It is shown.100% is reached to the rejection of haemocyte based on the blood separation sensing membrane prepared under above-mentioned condition, to blood lactase acid
Detection in, reached 125.8mA M-1Sensitivity and 0.01mM detectable limit.
Embodiment 2
(1) thiophene solution is prepared, (CN) containing KAu is prepared4And CuCl2The solion of solution, conducting polymer monomer are water-soluble
Liquid concentration is 0.01M, and reaction anion and reaction cation concn are 0.001M.
(2) supporter one end is sealed, the other end connects vacuum pump, and supporter is immersed in by environment temperature control at 40 DEG C
In solution A, taken out after 2min is filtered by vacuum, vacuum pressure 0.05MPa；Supporter is immersed in conducting polymer monomer water again
It is taken out after 3h in solution.The film naturally dry that will be prepared, film thickness reach 100nm.Based on the blood separation prepared under above-mentioned condition
Sensing membrane has reached 100% to the rejection of haemocyte, in the detection to blood lactase acid, has reached 91.0mA M-1Sensitivity
And the detectable limit of 0.1mM.
Embodiment 3
(1) aniline solution is prepared, prepares and contains K3Co(CN)6And NiCl2The solion of solution, conducting polymer monomer water
Solution concentration is 0.1M, and reaction anion and reaction cation concn are 0.01M.
(2) supporter one end is sealed, the other end connects vacuum pump, and supporter is immersed in by environment temperature control at 60 DEG C
In solution A, taken out after 5min is filtered by vacuum, vacuum pressure 0.08MPa；, then supporter is immersed in conducting polymer monomer
It is taken out after 2h in aqueous solution.The film prepared is dried, drying temperature is 40 DEG C, and film thickness reaches 500nm.Based on being made under above-mentioned condition
Standby blood separation sensing membrane has reached 100% to the rejection of haemocyte, in the detection to blood lactase acid, has reached 79.6mA
M-1Sensitivity and 0.5mM detectable limit.
Embodiment 4
(1) chromium solution is prepared, prepares and contains K3Fe(CN)6And FeCl3The solion of solution, conducting polymer monomer water
Solution concentration is 0.01M, and reaction anion and reaction cation concn are 0.001M.
(2) supporter one end is sealed, the other end connects vacuum pump, and environment temperature control submerges supporter at 35 DEG C molten
In liquid A, taken out after 1min is filtered by vacuum, vacuum pressure 0.1MPa；Supporter is immersed in the water of monomer containing conducting polymer again
It is taken out after 1h in solution.The film prepared is dried, drying temperature is 60 DEG C, and film thickness reaches 100nm.Based on being prepared under above-mentioned condition
Blood separation sensing membrane 100% has been reached to the rejection of haemocyte, in the detection to blood lactase acid, reached 106.4mA
M-1Sensitivity and 0.05mM detectable limit.
Embodiment 5
(1) chromium solution is prepared, prepares and contains K3Fe(CN)6And FeCl3The solion of solution, conducting polymer monomer water
Solution concentration is 0.1M, and reaction anion and reaction cation concn are 0.01M.
(2) supporter one end is sealed, the other end connects vacuum pump, and supporter is immersed in by environment temperature control at 35 DEG C
In solution A, taken out after 1min is filtered by vacuum, vacuum pressure 0.1MPa；Supporter is immersed in conducting polymer monomer water again
It is taken out after 1h in solution.The film naturally dry that will be prepared, film thickness reach 500nm.Based on the blood separation prepared under above-mentioned condition
Sensing membrane has reached 100% to the rejection of haemocyte, in the detection to blood lactase acid, has reached 134.6mA M-1Sensitivity
And the detectable limit of 0.01mM.
Claims (9)
1. a kind of preparation method of blood separation sensing membrane, specific step is as follows：
(1) preparation of reaction solution：Prepare conducting polymer monomer solution；It prepares containing reaction anion and reacts cation
Water solution A；Wherein the conducting polymer monomer solution is one of pyrroles, thiophene or aniline solution, reacts anion
For K3Fe(CN)6、KAu(CN)4Or K3Co(CN)6One of solution, the reaction cation is FeCl3、CuCl2Or NiCl2It is molten
One kind of liquid；
(2) deposition film on doughnut supporter：Doughnut supporter one end is sealed, the other end connects vacuum pump, will
Supporter is immersed in solution A, and vacuum filtration certain time takes out；Supporter is submerged into conducting polymer monomer solution again
In, certain time taking-up is stood, the preparation process of film is completed, and it is dry, obtain blood separation sensing membrane.
2. preparation method according to claim 1, it is characterised in that conducting polymer monomer solution is dense in step (1)
Degree is 0.01-0.1M.
3. preparation method according to claim 1, it is characterised in that the concentration of reaction anion is 0.001- in step (1)
0.01M, the concentration for reacting cation is 0.001-0.01M.
4. preparation method according to claim 1, it is characterised in that environment temperature control is at 25-60 DEG C in step (2).
5. preparation method according to claim 1, it is characterised in that vacuum pressure is 0.05-0.1MPa in step (2).
6. preparation method according to claim 1, it is characterised in that the vacuum filtration time in step (2) is 1-5min.
7. preparation method according to claim 1, it is characterised in that the time of repose in step (2) is 1-3h.
8. preparation method according to claim 1, it is characterised in that drying in step (2) is naturally dry or drying,
Drying temperature range is 40-60 DEG C.
9. preparation method according to claim 1, it is characterised in that blood separate sensing membrane with a thickness of 100-500nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810672975.7A CN108918239B (en) | 2018-06-26 | 2018-06-26 | Preparation method of blood separation sensing membrane |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810672975.7A CN108918239B (en) | 2018-06-26 | 2018-06-26 | Preparation method of blood separation sensing membrane |
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| Publication Number | Publication Date |
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| CN108918239A true CN108918239A (en) | 2018-11-30 |
| CN108918239B CN108918239B (en) | 2021-06-22 |
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| CN201810672975.7A Active CN108918239B (en) | 2018-06-26 | 2018-06-26 | Preparation method of blood separation sensing membrane |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105013335A (en) * | 2015-06-29 | 2015-11-04 | 天津工业大学 | Polymer conductive composite membrane and preparation method thereof |
| CN104437135B (en) * | 2014-12-29 | 2016-03-16 | 中科院广州化学有限公司 | A kind of hydrophilic polymer modified fibre compound forward osmosis membrane and method for making thereof and application |
| CN105628756A (en) * | 2015-12-30 | 2016-06-01 | 南京工业大学 | Preparation method of blood synchronous separation and sensing film |
-
2018
- 2018-06-26 CN CN201810672975.7A patent/CN108918239B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104437135B (en) * | 2014-12-29 | 2016-03-16 | 中科院广州化学有限公司 | A kind of hydrophilic polymer modified fibre compound forward osmosis membrane and method for making thereof and application |
| CN105013335A (en) * | 2015-06-29 | 2015-11-04 | 天津工业大学 | Polymer conductive composite membrane and preparation method thereof |
| CN105628756A (en) * | 2015-12-30 | 2016-06-01 | 南京工业大学 | Preparation method of blood synchronous separation and sensing film |
Non-Patent Citations (2)
| Title |
|---|
| 储震宇 等: "用于血液分离与检测同步的分离传感膜的制备", 《2016年中国-欧盟医药生物膜科学与技术研讨会论文集》 * |
| 周磊: "聚乙酰苯胺-普鲁士蓝杂化膜材料的制备及其在过氧化氢检测中的应用", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
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| CN108918239B (en) | 2021-06-22 |
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