CN108917255A - A kind of superelevation prompt drop Thawing Methods and device - Google Patents
A kind of superelevation prompt drop Thawing Methods and device Download PDFInfo
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- CN108917255A CN108917255A CN201810743821.2A CN201810743821A CN108917255A CN 108917255 A CN108917255 A CN 108917255A CN 201810743821 A CN201810743821 A CN 201810743821A CN 108917255 A CN108917255 A CN 108917255A
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000010257 thawing Methods 0.000 title claims abstract description 7
- 239000000523 sample Substances 0.000 claims abstract description 55
- 230000000087 stabilizing effect Effects 0.000 claims abstract description 11
- 230000009471 action Effects 0.000 claims abstract description 6
- 239000012472 biological sample Substances 0.000 claims abstract description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 27
- 229910052757 nitrogen Inorganic materials 0.000 claims description 15
- 238000001816 cooling Methods 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 6
- 230000007774 longterm Effects 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 238000005538 encapsulation Methods 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 239000004411 aluminium Substances 0.000 claims description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 2
- 229910052782 aluminium Inorganic materials 0.000 claims description 2
- 239000002504 physiological saline solution Substances 0.000 claims description 2
- 230000008676 import Effects 0.000 claims 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 238000003860 storage Methods 0.000 abstract description 3
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 9
- 239000012620 biological material Substances 0.000 description 7
- 230000006378 damage Effects 0.000 description 7
- 238000005138 cryopreservation Methods 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 208000027418 Wounds and injury Diseases 0.000 description 4
- 238000009835 boiling Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000009477 glass transition Effects 0.000 description 4
- 208000014674 injury Diseases 0.000 description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000013078 crystal Substances 0.000 description 3
- 230000008646 thermal stress Effects 0.000 description 3
- 210000005239 tubule Anatomy 0.000 description 3
- 238000003466 welding Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000004880 explosion Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- -1 polytetrafluoroethylene Polymers 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
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- 238000010276 construction Methods 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004031 devitrification Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 210000000887 face Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
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Classifications
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
- F25D3/10—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F28—HEAT EXCHANGE IN GENERAL
- F28D—HEAT-EXCHANGE APPARATUS, NOT PROVIDED FOR IN ANOTHER SUBCLASS, IN WHICH THE HEAT-EXCHANGE MEDIA DO NOT COME INTO DIRECT CONTACT
- F28D15/00—Heat-exchange apparatus with the intermediate heat-transfer medium in closed tubes passing into or through the conduit walls ; Heat-exchange apparatus employing intermediate heat-transfer medium or bodies
Abstract
A kind of superelevation prompt drop Thawing Methods and device, the glassy state storage for biological samples such as cell, tissues.Device includes chip assembly and outer box, and chip assembly is placed in outer box.Chip assembly is flat hollow structure, and inside is sample microcavity, for holding biological sample to be saved；Maximum two side external surface of area is carved with microchannel or micro-pillar array on chip assembly, is used for heat exchange.Outer box are all flat hollow structure, and inside is chip chamber for holding chip assembly；Chip chamber side is connected to working medium entrances by microwell array, pressure stabilizing cavity, and the other side is connected to sender property outlet；Microwell array is right against microchannel or the micro-pillar array on chip assembly surface, and there are a jet stream gaps therebetween.After working medium injection of the present invention, it is injected on chip assembly by microwell array, under the double action of microjet and microchannel, forms superpower surface heat exchanging effect, realize the superelevation prompt drop rewarming of inner sample.
Description
Technical field
The invention belongs to field of biomedicine technology, and in particular to a kind of ultrahigh speed of biological material vitrified cryo-conservation
Thawing Methods and device drop.
Background technique
Cryo-conservation is unique effective means of the biomaterials long-term preservations such as current numerous cells, tissue.Biomaterial
Although can be saved in long-term safety under ultralow temperature, in cooling (by room temperature to ultralow temperature) and rewarming (by ultralow temperature to room temperature)
Link is very likely by serious damage, i.e., so-called low temperature injury.About the mechanism of low temperature injury, educational circles is generally accepted carefully
The main reason for damage of eucaryotic cell structure caused by outer ice-crystal growth intracellular and solute concentration variation etc. are low temperature injuries.
" glassy state storage method " is a kind of ideal Cryopreservation proposed in recent years, is referred to by promoting biology
Glass transition (forming non-crystal glassy state) occurs in temperature-fall period for material, and intraor extracellular ice-crystal growth is avoided to cause
Low temperature injury.But the glass transition of biomaterial needs the rate of temperature fall of superelevation, only specifically faces when rate of temperature fall is greater than
Glass transition could occur for biomaterial when boundary's rate of temperature fall；Moreover, rewarming rate also must be high during rewarming
Just it is avoided that recrystallization phenomenon occurs for vitrified solution in critical rewarming rate.
For the drop rewarming rate of superelevation needed for obtaining glass transition, existing glassy state storage method is usually using straw
(French mini-Straw, 0.25ml) carries micro-example and direct plunge into Liquid Nitrogen (- 196 DEG C), by between sample and liquid nitrogen
The huge temperature difference and liquid nitrogen tubule surface vaporization driving sample cool down rapidly；In rewarming link, then by tubule
It direct plunges into water bath with thermostatic control (30~40 DEG C), is rapidly heated with the temperature difference driving sample between sample and warm water, on this basis,
Vajta et al. improves the structure of tubule, proposes open stretched thin-tube method (Open Pulled Straw, OPS), gained
To rate of temperature fall greatly improve.Reubinnoff also applies this method and is successfully made stem cell vitrifying low temperature for the first time
It saves, follow-up study is continuously improved this method, seal type stretched thin-tube method (Closed successively occurs
Pulled Straw, CPS), micro- suction nozzle method (Micropipette), the methods of Cryotop and Cryotip.These vitrifyings are low
Warm store method and device are applied successfully in some fields, but there are still larger deficiencies, including following several respects.
1) heat exchange efficiency is limited.Existing cool-down method is all essentially the pool boiling by sample in liquid nitrogen
(Pool boiling) obtains higher rate of temperature fall, but can generate a large amount of liquid nitrogen vapor during pool boiling and be wrapped in sample
Around product, heat-insulated effect is generated to further cooling, therefore heat exchange efficiency is lower；Existing Thawing Methods generally pass through sample
The temperature difference between rewarming liquid drives heating, and heat exchange efficiency is lower, and as sample temperature gos up, the temperature difference reduces, rewarming speed
Rate decays rapidly, it is easy to the problems such as generating devitrification.
2) sample volume is limited serious.Sample is in hemisphere or cylindrical body in existing method, with the increasing of sample volume
Add, the heat-transfer path of sample interior increases sharply, and can be obtained maximum rate of temperature fall and is greatly reduced, and sample interior drops
Warm uniformity cannot guarantee that.
3) manual operations is relied on, treatment process is difficult to ensure standardization and consistency, and also heavy dependence operates processing result
Personnel's experience and technology.
Summary of the invention
It is an object of the invention to overcome the problems in existing vitrifying cryo-conservation technology, providing one kind be can be realized fastly
The method and device of the mild rewarming of prompt drop.
In order to solve the above technical problems, the present invention uses following technical scheme：
A kind of superelevation prompt drop rewarming apparatus, including chip assembly and outer box, chip assembly are located in outer box；Chip assembly is
Flat hollow structure, inside is sample microcavity, for holding biological sample to be saved；On chip assembly simultaneously be arranged sample into
Outlet is connected to sample microcavity, for biological sample to be filled or recycled into chip；The maximum two sides appearance of area on chip assembly
Microchannel or micro-pillar array are carved in face, are used for heat exchange；Outer box are all flat hollow structure, and inside is chip chamber for holding core
Piece component；Chip chamber side is connected to working medium entrances by microwell array, pressure stabilizing cavity, and the other side is connected to sender property outlet；Microwell array
It is right against microchannel or the micro-pillar array on chip assembly surface, and there are a jet stream gaps therebetween.
When the superelevation prompt drop rewarming apparatus is for when saving the solid samples such as histotomy, the chip assembly to be can be used
The structure of on-fixed encapsulation, including upper layer chip and lower layer chip, and the outer surface of upper layer chip and lower layer chip are carved with micro-
The inner surface of channel or micro-pillar array, upper layer chip and lower layer chip is then adjacent to sample to be saved and forms sandwich structure, sets
In the outer box.
The chip assembly is made of highly heat-conductive materials such as copper, aluminium, and the inner surface contacted with sample is silver-plated, thickness of coating
Greater than 1 micron；The depth-to-width ratio of microchannel or micro-pillar array is greater than 1, and groundwork thickness is less than 0.5 millimeter.
The microchannel or micro-pillar array are rectangle or trapezoidal flat microchannel array, or are micro- cylindrical-array, micro- square column
Array, micro- triangular prism array, hydraulic characteristic size are respectively less than 1 millimeter, and array format can be parallel array or staggered.
The outer box material is soft or hard polytetrafluoroethylene (PTFE), and brittle temperature is lower than subzero 200 degrees Celsius.
The working medium includes cooling working medium and rewarming working medium, wherein cooling working medium is liquid nitrogen, temperature is Celsius lower than subzero 196
Degree, rewarming working medium are the alcohol water blend of 75~90% concentration, or are physiological saline, and temperature is 30 to 40 degrees Celsius.
A kind of superelevation prompt drop Thawing Methods, include the following steps：
1) cell suspending liquid to be saved is injected in the microcavity of chip assembly, or biological tissue section to be saved is set
Sandwich structure is formed between the chip of the upper and lower.
2) chip assembly is placed in outer box, it is ensured that the microchannel of chip assembly outer surface or micro-pillar array and outer box it is micro-
Hole array position face.
3) the working medium high speed that cools down is imported to the working medium entrances of outer box, the working medium that cools down is distributed in micropore through pressure stabilizing cavity even action
Array forms microjet impact on the microchannel on chip assembly surface or micro-pillar array, then the sender property outlet stream through outer box
Out, the heat in chip assembly and sample is taken away, sample high speed is promoted to cool down.
4) after the completion of cooling down, outer box is transferred in liquid nitrogen container together with chip assembly and sample and carry out long-term preservation.
5) when needing using sample, outer box are taken out from liquid nitrogen container, and rewarming working medium high speed is imported to the work of outer box
Matter entrance, rewarming working medium form microjet through pressure stabilizing cavity and microwell array and act on chip assembly surface, promote chip assembly
With internal sample rapid rewarming.
6) chip is taken out from outer box, and cell suspending liquid or biological tissue are recycled from chip.
Compared with prior art, the beneficial effects of the invention are as follows：
1) heat exchange efficiency is promoted, and drop rewarming rate is high.The present invention comprehensively utilizes microjet and Thermal Performance of Micro Channels principle, passes through
Multiple heat hydrodynamic interaction under minute yardstick generates high heat exchanger efficiency, and relatively existing pool boiling technology has larger excellent
Gesture is more advantageous to the drop of superelevation needed for realizing vitrifying cryo-conservation, rewarming rate.
2) uniformity of temperature profile, thermal stress is small in sample.The present invention passes through the design of pressure stabilizing cavity and microwell array, realizes drop
Warm working medium (liquid nitrogen) and rewarming working medium uniformly act on chip surface, cool down equal with the Temperature Distribution in sample during rewarming
It is even, avoid damage caused by temperature gradient and thermal stress in biological sample.
3) favorable expandability has a wide range of application.Since chip in the present invention and sample are in flat structure, and surface heat exchanging is imitated
Rate is uniform, drop heat transfer during rewarming be approximately one dimensional heat transfer (i.e. heat transfer is concentrated mainly on thickness direction, and with width
Spend unrelated with length direction), thus can flexible Application different in width or length chip acquisition different volumes sample ultrahigh speed
Rewarming drops.
4) drop rewarming process consistency is good.The effect of device and method of the present invention does not depend on manual operations, is conducive to
Realization process consistency.
Detailed description of the invention
Fig. 1 is the perspective view of the explosion of the superelevation prompt drop rewarming apparatus overall structure in the embodiment of the present invention；
Fig. 2 is chip modular construction perspective view of the explosion in the superelevation prompt drop rewarming apparatus in the embodiment of the present invention；
Fig. 3 is the diagrammatic cross-section of the superelevation prompt drop rewarming apparatus overall structure in the embodiment of the present invention；
Fig. 4 is based on the cooling and rewarming rate test result in the embodiment of the present invention.
In figure, 1 is chip assembly, and 1a is the upper layer chip for constituting chip assembly, and 1b be the lower layer chip of composition chip assembly, 11
It is sample export for sample inlet, 12,13 be sample microcavity, and 2 be outer box, and 21 be chip chamber, and 22 be microwell array, and 23 be pressure stabilizing
Chamber, 24 be working medium entrances, and 25 be sender property outlet.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, not
For limiting the present invention.
As shown in Figure 1, the superelevation prompt drop rewarming apparatus in the present embodiment includes chip assembly 1 and outer box 2.Chip assembly 1
It is formed by chip 1a and 1b by laser welding, as shown in Fig. 2, wherein 1a is with a thickness of 1 millimeter, 1b is with a thickness of 1.5 millimeters, material
It is copper.Parallel microchannels array is carved in a side surface of chip 1a, and channel depth is 0.75 millimeter, and width is 0.25 millimeter,
Channel wall thickness is 0.25 millimeter；Same micro channel array is carved in a side surface of chip 1b, and it is 20 millis that width is carved in the other side
Rice, the square groove that length is 10 millimeters, depth is 0.5 millimeter, and the slot both ends are all connected with the through-hole through chip.In welding fabrication
Before, chip 1a and 1b are silver-plated, and thickness of coating is 1 micron.After welding, the square groove is formed for storing cell suspending liquid
Microcavity 13, the through-hole being connected to microcavity 13 is then respectively as sample inlet 11 and outlet 12.
Outer box 2 are similarly flat hollow, and material is polytetrafluoroethylene (PTFE).And outer box are in symmetrical structure, including three A, B, C couple
Title face, as shown in figure 3, being only described herein with regard to one side feature.Outer box inner core piece chamber 21 is used to hold chip assembly 1,
And chip chamber 21 slightly greater height than chip assembly 1 thickness, after being packed into chip assembly 1 above chip with Jet stream array it
Between formed jet stream gap.Chip chamber 21 is connected to working medium entrances 24, left and right two with pressure stabilizing cavity 23 by the microwell array 22 of upper and lower sides
Side is connected to sender property outlet 25.
When work, cell suspending liquid to be saved enters the sample microcavity 13 of chip assembly 1 from biomaterial entrance 11,
Under the restriction effect in 13 space of sample microcavity, biomaterial is present in sample microcavity 13 with form of liquid film.Subsequent chip assembly 1
It is placed into the chip chamber 21 of outer box 2, and liquid nitrogen is injected to 24 high speed of working medium entrances of outer box by working medium drive system.?
Under the action of pressure stabilizing cavity 23, liquid nitrogen is injected in the micro- of chip assembly 1 uniformly across microwell array 22 in the form of Jet stream array
On channel array.Under the double action of microjet and microchannel, superelevation and uniform surface heat exchanging effect are formed, by chip battle array
The heat of column 1 and wherein cell suspending liquid is taken away rapidly, is discharged with liquid nitrogen through sender property outlet 25, cell suspending liquid fast cooling.
When needed after the save is completed by sample rewarming, then rewarming alcoholic solution high speed is injected to the working medium of outer box by working medium drive system
Entrance 24 equally promotes sample rapid rewarming under the double action of microjet and microchannel.Since cell suspending liquid film has
Biggish specific surface area, therefore it is easy to get higher cooling and rewarming rate.Moreover, either surface is changed during drop rewarming
Thermal resistance or internal thermal conduction resistance are all approached in different parts and are uniformly distributed, and form one dimensional heat transfer effect, the temperature difference in sample
Thermal stress is small.
Test results are shown in figure 4 for the drop rewarming of device, it is seen that arrives subzero 130 across 0 degree Celsius when sample is in cooling
Section (the i.e. so-called dangerous temperature section) used time only 0.2 second is spent, average rate of temperature fall is up to 39000 DEG C/min；During rewarming
It is about 0.19 second across the dangerous temperature section used time, average too 41000 degree DEG C/min of rewarming rate.It is provided according to disclosed document
Expect, about 23000 DEG C/min of rate of temperature fall of the best Cryotop device of the drop rewarming performance applied on Present clinical, rewarming speed
About 42000 DEG C/min of rate, sample load is at 1 microlitre or less.Comparison shows that the present invention can be realized preferably drop rewarming performance,
And sample load (100 microlitres) is significantly larger than Cryotop, can be suitable for further types of sample, and to operating process and
The requirement of operative skill substantially reduces, and has larger performance advantage compared with the prior art.
The micro channel array of chip surface described in the present embodiment can transform to micro-pillar array, further increase surface heat exchanging
Uniformity；The form of microtrabeculae can be cylinder, square column or triangular prism etc..
When the preservation for the continuous sheet such as cornea, skin, ovary tissue or strip object, chip described in the present embodiment
The form of on-fixed encapsulation can be used in component.
Those of ordinary skill in the art will understand that the embodiments described herein, which is to help reader, understands this hair
Bright principle, it should be understood that protection scope of the present invention is not limited to such specific embodiments and embodiments.This field
Those of ordinary skill disclosed the technical disclosures can make the various other tools for not departing from essence of the invention according to the present invention
Body variations and combinations, these variations and combinations are still within the scope of the present invention.
Claims (7)
1. a kind of superelevation prompt drop rewarming apparatus, it is characterised in that：The superelevation prompt drop rewarming apparatus includes chip assembly and outer box,
Chip assembly is located in outer box；Chip assembly is flat hollow structure, and inside is sample microcavity, for holding biology to be saved
Sample；Sample inlet and outlet are set simultaneously on chip assembly, are connected to sample microcavity, for biological sample to be filled or recycled into chip
Product；Maximum two side external surface of area is carved with microchannel or micro-pillar array on chip assembly, is used for heat exchange；Outer box are all flat
Hollow structure, inside are chip chamber for holding chip assembly；Chip chamber side is connected to working medium by microwell array, pressure stabilizing cavity and enters
Mouthful, the other side is connected to sender property outlet；Microwell array is right against microchannel or the micro-pillar array on chip assembly surface, and therebetween
There are a jet stream gaps.
2. superelevation prompt drop rewarming apparatus according to claim 1, it is characterised in that：When the superelevation prompt drop rewarming apparatus is used for
When saving the non-liquid sample such as histotomy, the chip assembly can be used the structure of on-fixed encapsulation, including upper layer chip and
Lower layer chip, and microchannel or micro-pillar array, upper layer chip and lower layer's core are carved in the outer surface of upper layer chip and lower layer chip
The inner surface of piece is then adjacent to sample to be saved and forms sandwich structure, is placed in the outer box.
3. superelevation prompt drop rewarming apparatus according to claim 1, it is characterised in that：The chip assembly by copper, aluminium is contour leads
Hot material is made, and the inner surface contacted with sample is silver-plated, and thickness of coating is greater than 1 micron；The deep width of microchannel or micro-pillar array
Than being greater than 1, groundwork thickness is less than 0.5 millimeter.
4. superelevation prompt drop rewarming apparatus according to claim 1, it is characterised in that：The microchannel or micro-pillar array are square
Shape or trapezoidal flat microchannel array, or be micro- cylindrical-array, micro- square column array, micro- triangular prism array, hydraulic characteristic size is equal
Less than 1 millimeter, array format can be parallel array or staggered.
5. superelevation prompt drop rewarming apparatus according to claim 1, it is characterised in that：The outer box material is that soft or hard is poly-
Tetrafluoroethene, brittle temperature are lower than subzero 200 degrees Celsius.
6. superelevation prompt drop rewarming apparatus according to claim 1, it is characterised in that：The working medium includes cooling working medium and rewarming
Working medium, wherein cooling working medium is liquid nitrogen, temperature is lower than subzero 196 degrees Celsius, and rewarming working medium is the alcohol water of 75~90% concentration
Solution, or be physiological saline, temperature is 30 to 40 degrees Celsius.
7. a kind of superelevation prompt drop Thawing Methods, which is characterized in that include the following steps：
1) cell suspending liquid to be saved is injected in the microcavity of chip assembly, or biological tissue section to be saved is placed in
Sandwich structure is formed between layer and lower layer chip；
2) chip assembly is placed in outer box, it is ensured that the micropore battle array of the microchannel of chip assembly outer surface or micro-pillar array and outer box
Column position face；
3) the working medium high speed that cools down is imported to the working medium entrances of outer box, the working medium that cools down is distributed in micropore battle array through pressure stabilizing cavity even action
Column form microjet impact on the microchannel on chip assembly surface or micro-pillar array, then the sender property outlet outflow through outer box,
The heat in chip assembly and sample is taken away, sample high speed is promoted to cool down；
4) after the completion of cooling down, outer box is transferred in liquid nitrogen container together with chip assembly and sample and carry out long-term preservation；
5) when needing using sample, outer box are taken out from liquid nitrogen container, and the working medium that rewarming working medium high speed imports outer box is entered
Mouthful, rewarming working medium forms microjet through pressure stabilizing cavity and microwell array and acts on chip assembly surface, promotes chip assembly and interior
The sample rapid rewarming in portion；
6) chip is taken out from outer box, and cell suspending liquid or biological tissue are recycled from chip.
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| CN201810743821.2A CN108917255A (en) | 2018-07-09 | 2018-07-09 | A kind of superelevation prompt drop Thawing Methods and device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810743821.2A CN108917255A (en) | 2018-07-09 | 2018-07-09 | A kind of superelevation prompt drop Thawing Methods and device |
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| CN201810743821.2A Pending CN108917255A (en) | 2018-07-09 | 2018-07-09 | A kind of superelevation prompt drop Thawing Methods and device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111146544A (en) * | 2019-12-30 | 2020-05-12 | 电子科技大学 | Efficient cooling structure for small high-power millimeter wave device |
| WO2021129312A1 (en) * | 2019-12-24 | 2021-07-01 | 上海明悦医疗科技有限公司 | Carrier, vacuumizing device and tissue cryopreservation system |
| CN113403191A (en) * | 2021-06-09 | 2021-09-17 | 上海理工大学 | Modularized low-temperature preservation and rapid rewarming device for biological samples |
| CN115777684A (en) * | 2021-09-10 | 2023-03-14 | 深圳拜尔洛克生物技术有限公司 | Device for cryopreservation or thawing recovery of biological tissue |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113403191A (en) * | 2021-06-09 | 2021-09-17 | 上海理工大学 | Modularized low-temperature preservation and rapid rewarming device for biological samples |
| CN115777684A (en) * | 2021-09-10 | 2023-03-14 | 深圳拜尔洛克生物技术有限公司 | Device for cryopreservation or thawing recovery of biological tissue |
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