CN108913743A - A kind of extracting method of corn protein peptide - Google Patents
A kind of extracting method of corn protein peptide Download PDFInfo
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- CN108913743A CN108913743A CN201810860907.3A CN201810860907A CN108913743A CN 108913743 A CN108913743 A CN 108913743A CN 201810860907 A CN201810860907 A CN 201810860907A CN 108913743 A CN108913743 A CN 108913743A
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention discloses a kind of extracting methods of corn protein peptide, include the following steps, will obtain corn germ cake after maize germ degreasing, and corn germ cake is put into pulverizer and is crushed, and after crushing after 80-100 mesh, being configured to protein concentration is 8-12%（w/w）Corn germ cake mixed liquor, alkali protease Promod523P liquid is added, the every 10g enzymolysis liquid of additive amount adds 1.0-1.5 μ L, and enzymolysis liquid pH value is 8-9 at this time, 30-55 DEG C of temperature controlled water baths, constant temperature 40 minutes, the dissolution of albumen is carried out, forms protein dissolution liquid, then neutral proteinase is added in protein dissolution liquid, additive amount is that 10g protein dissolution liquid adds 0.7-1.5 μ L, and pH value is 4.2-5.2 at this time, continues to be kept for 35-45 DEG C of temperature 15 minutes.The present invention improves the quality and activity of product, and more safe and healthy, extraction process is mature, is suitble to promote.
Description
Technical field
The present invention relates to corn protein peptide extractive technique field more particularly to a kind of extracting methods of corn protein peptide.
Background technique
Corn is annual monoecism cross-pollinatd plant, and plant is tall and big, and stem is strong, is important cereal crops and feeding
Material crop and the highest crops of whole world total output, cultivated area and total output are only second to rice and wheat.Corn one
Directly all it is known as life prolonging food, protein rich in, fat, vitamin, microelement, cellulose etc. have exploitation high
Nutrition, high biological function food great potential.But since its heredity is complex, variation type is abundant, in routine
In breeding process there is excessive cycle, the coefficient of variation is excessive, influences the shortcomings that offspring development, and modern biotechnology breeding skill
Art not only overcomes disadvantages mentioned above and deficiency, while also improving breeding speed and quality.
Through retrieving, Patent No. CN201310457453.2 proposes a kind of side that albumen powder is extracted from corn germ cake
Method, with 85 DEG C of pasteurization in the patent, high temperature is easy that zein is made to lose activity at the very start, to influence product
Quality, for this purpose, the present invention proposes a kind of extracting method of corn protein peptide.
Summary of the invention
The purpose of the present invention is to solve disadvantage existing in the prior art, and a kind of corn protein peptide for proposing mentions
Take method.
To achieve the goals above, present invention employs following technical solutions：
A kind of extracting method of corn protein peptide, includes the following steps,
S1 will obtain corn germ cake after maize germ degreasing, by corn germ cake be put into pulverizer crush, after crushing by
It after 80-100 mesh, adds water and stirs uniformly, being configured to protein concentration is 8-12%（w/w）Corn germ cake mixed liquor, Pasteur
Disinfection, the corn germ cake mixed liquor A after being sterilized；
S2, by the NaOH solution tune pH value of solution of 0.09mol/l to 7.8-8.2 of the A in S1；
Compound protease is added in S3, and additive amount is the 0.05% of corn germ cake mixed liquor weight, slowly stirs at 30-50 DEG C of heat preservation
It mixes, mixing speed is that 15-22 turns/min, maintains 2h, is digested, obtain enzymolysis liquid B；
The enzymolysis liquid B that S4, S3 are obtained, is added alkali protease Promod523P liquid, and the every 10g enzymolysis liquid of additive amount adds 1.0-
1.5 μ L, enzymolysis liquid pH value is 8-9 at this time, 30-55 DEG C of temperature controlled water baths, constant temperature 40 minutes, carries out the dissolution of albumen, is formed
Protein dissolution liquid, is then added neutral proteinase in protein dissolution liquid, and additive amount is that 10g protein dissolution liquid adds 0.7-1.5 μ
L, pH value is 4.2-5.2 at this time, continues to be kept for 35-45 DEG C of temperature 15 minutes；
S5, will in S4 gained the enzyme deactivation in 100 DEG C of water-baths of protein dissolution liquid, is placed after enzyme deactivation ice water water-bath 1-2 minutes cooling
To 25 DEG C；
Protein dissolution liquid obtained by S5 is put into centrifuge centrifuge separation, centrifuge speed 4000r/min, centrifugation time by S6
10min takes clear liquid to use；
S7, clear liquid evaporate moisture by heating, obtain the sticky albumen powder of moisture content 18-22%, dry using 80 DEG C of baking ovens of temperature
Albumen peptide product is obtained after dry.
Preferably, in the S1, the concrete operations of the pasteurization are：Corn germ cake mixed liquor is put into 68-70
Corn germ cake mixed liquor of the rapid cooling to 4-5 DEG C, after being sterilized after heating 30min at a temperature of DEG C.
Preferably, in the S2, by the NaOH solution tune pH value of solution of 0.09mol/l to 8.0 of the A in S1.
Preferably, in the S4, alkali protease Promod523P liquid is added in the enzymolysis liquid B that S3 is obtained, and additive amount is every
10g enzymolysis liquid adds 1.25 μ L, and enzymolysis liquid pH value is 8.5 at this time, 42.5 DEG C of temperature controlled water baths, constant temperature 40 minutes, carries out egg
White dissolution forms protein dissolution liquid, and neutral proteinase is then added in protein dissolution liquid, and additive amount is 10g protein dissolution
Liquid adds 1.1 μ L, and pH value is 4.7 at this time, continues to be kept for 40 DEG C of temperature 15 minutes.
Preferably, in the S7, clear liquid evaporates moisture by heating, obtains the sticky albumen powder of moisture content 20%, then pass through
Albumen peptide product is obtained after 80 DEG C of baking oven drying of excess temperature.
Preferably, S5, will in S4 gained the enzyme deactivation in 100 DEG C of water-baths of protein dissolution liquid, after enzyme deactivation place ice water water-bath 2
Minute is cooled to 25 DEG C.
The extracting method of a kind of corn protein peptide proposed by the present invention, by the way that maize germ will be obtained after maize germ degreasing
Corn germ cake is put into pulverizer and crushed by the dregs of rice, after crushing after 80-100 mesh, is added water and stirred uniformly, is configured to albumen
Matter concentration is 8-12%（w/w）Corn germ cake mixed liquor, pasteurization, the corn germ cake mixed liquor A after being sterilized,
The concrete operations of pasteurization are：Rapid cooling after heating 30min at a temperature of corn germ cake mixed liquor is put into 68-70 DEG C
To 4-5 DEG C, the corn germ cake mixed liquor after being sterilized is easy to make protein denaturation, influences to produce when temperature is higher than 72 DEG C
The quality of object, the present invention improve the quality and activity of product, and more safe and healthy, extraction process is mature, is suitble to promote.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.
Embodiment one
A kind of extracting method of corn protein peptide proposed by the present invention, includes the following steps,
S1 will obtain corn germ cake after maize germ degreasing, and corn germ cake is put into pulverizer and is crushed, by 80 after crushing
It after mesh, adds water and stirs uniformly, being configured to protein concentration is 8%（w/w）Corn germ cake mixed liquor, pasteurization obtains
Corn germ cake mixed liquor A after disinfection；
S2, by the NaOH solution tune pH value of solution of 0.09mol/l to 7.8 of the A in S1；
Compound protease is added in S3, and additive amount is the 0.05% of corn germ cake mixed liquor weight, is slowly stirred at 30 DEG C of heat preservation,
Mixing speed is 15 turns/min, maintains 2h, is digested, obtain enzymolysis liquid B；
The enzymolysis liquid B that S4, S3 are obtained, is added alkali protease Promod523P liquid, and the every 10g enzymolysis liquid of additive amount adds 1.25 μ
L, enzymolysis liquid pH value is 8.5 at this time, 42.5 DEG C of temperature controlled water baths, constant temperature 40 minutes, carries out the dissolution of albumen, it is molten to form albumen
Liquid is solved, neutral proteinase is then added in protein dissolution liquid, additive amount is that 10g protein dissolution liquid adds 1.1 μ L, at this time pH value
It is 4.7, continues to be kept for 40 DEG C of temperature 15 minutes；
S5, will in S4 gained the enzyme deactivation in 100 DEG C of water-baths of protein dissolution liquid, place ice water water-bath 1 minute and be cooled to after enzyme deactivation
25℃；
Protein dissolution liquid obtained by S5 is put into centrifuge centrifuge separation, centrifuge speed 4000r/min, centrifugation time by S6
10min takes clear liquid to use；
S7, clear liquid evaporate moisture by heating, obtain the sticky albumen powder of moisture content 18%, using 80 DEG C of baking oven drying of temperature
After obtain albumen peptide product.
Embodiment two
A kind of extracting method of corn protein peptide proposed by the present invention, includes the following steps,
S1 will obtain corn germ cake after maize germ degreasing, by corn germ cake be put into pulverizer crush, after crushing by
It after 80-100 mesh, adds water and stirs uniformly, being configured to protein concentration is 12%（w/w）Corn germ cake mixed liquor, Pasteur disappears
Poison, the corn germ cake mixed liquor A after being sterilized；
S2, by the NaOH solution tune pH value of solution of 0.09mol/l to 8.2 of the A in S1；
Compound protease is added in S3, and additive amount is the 0.05% of corn germ cake mixed liquor weight, is slowly stirred at 50 DEG C of heat preservation,
Mixing speed is 22 turns/min, maintains 2h, is digested, obtain enzymolysis liquid B；
The enzymolysis liquid B that S4, S3 are obtained, is added alkali protease Promod523P liquid, and the every 10g enzymolysis liquid of additive amount adds 1.25 μ
L, enzymolysis liquid pH value is 8.5 at this time, 42.5 DEG C of temperature controlled water baths, constant temperature 40 minutes, carries out the dissolution of albumen, it is molten to form albumen
Liquid is solved, neutral proteinase is then added in protein dissolution liquid, additive amount is that 10g protein dissolution liquid adds 1.1 μ L, at this time pH value
It is 4.7, continues to be kept for 40 DEG C of temperature 15 minutes；
S5, will in S4 gained the enzyme deactivation in 100 DEG C of water-baths of protein dissolution liquid, is placed after enzyme deactivation ice water water-bath 1-2 minutes cooling
To 25 DEG C；
Protein dissolution liquid obtained by S5 is put into centrifuge centrifuge separation, centrifuge speed 4000r/min, centrifugation time by S6
10min takes clear liquid to use；
S7, clear liquid evaporate moisture by heating, obtain the sticky albumen powder of moisture content 22%, using 80 DEG C of baking oven drying of temperature
After obtain albumen peptide product.
Embodiment three
A kind of extracting method of corn protein peptide proposed by the present invention, includes the following steps,
S1 will obtain corn germ cake after maize germ degreasing, and corn germ cake is put into pulverizer and is crushed, by 90 after crushing
It after mesh, adds water and stirs uniformly, being configured to protein concentration is 10%（w/w）Corn germ cake mixed liquor, pasteurization obtains
Corn germ cake mixed liquor A after to disinfection；
S2, by the NaOH solution tune pH value of solution of 0.09mol/l to 8.0 of the A in S1；
Compound protease is added in S3, and additive amount is the 0.05% of corn germ cake mixed liquor weight, is slowly stirred at 40 DEG C of heat preservation,
Mixing speed is 18 turns/min, maintains 2h, is digested, obtain enzymolysis liquid B；
The enzymolysis liquid B that S4, S3 are obtained, is added alkali protease Promod523P liquid, and the every 10g enzymolysis liquid of additive amount adds 1.25 μ
L, enzymolysis liquid pH value is 8.5 at this time, 42.5 DEG C of temperature controlled water baths, constant temperature 40 minutes, carries out the dissolution of albumen, it is molten to form albumen
Liquid is solved, neutral proteinase is then added in protein dissolution liquid, additive amount is that 10g protein dissolution liquid adds 1.1 μ L, at this time pH value
It is 4.7, continues to be kept for 40 DEG C of temperature 15 minutes；
S5, will in S4 gained the enzyme deactivation in 100 DEG C of water-baths of protein dissolution liquid, is placed after enzyme deactivation ice water water-bath 1.5 minutes cooling
To 25 DEG C；
Protein dissolution liquid obtained by S5 is put into centrifuge centrifuge separation, centrifuge speed 4000r/min, centrifugation time by S6
10min takes clear liquid to use；
S7, clear liquid evaporate moisture by heating, obtain the sticky albumen powder of moisture content 20%, using 80 DEG C of baking oven drying of temperature
After obtain albumen peptide product.
The extracting method of a kind of corn protein peptide proposed by the present invention, by the way that maize germ will be obtained after maize germ degreasing
Corn germ cake is put into pulverizer and crushed by the dregs of rice, after crushing after 80-100 mesh, is added water and stirred uniformly, is configured to albumen
Matter concentration is 8-12%（w/w）Corn germ cake mixed liquor, pasteurization, the corn germ cake mixed liquor A after being sterilized,
The concrete operations of pasteurization are：Rapid cooling after heating 30min at a temperature of corn germ cake mixed liquor is put into 68-70 DEG C
To 4-5 DEG C, the corn germ cake mixed liquor after being sterilized is easy to make protein denaturation, influences to produce when temperature is higher than 72 DEG C
The quality of object, the present invention improve the quality and activity of product, and more safe and healthy, extraction process is mature, is suitble to promote.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of extracting method of corn protein peptide, which is characterized in that include the following steps,
S1 will obtain corn germ cake after maize germ degreasing, by corn germ cake be put into pulverizer crush, after crushing by
It after 80-100 mesh, adds water and stirs uniformly, being configured to protein concentration is 8-12%（w/w）Corn germ cake mixed liquor, Pasteur
Disinfection, the corn germ cake mixed liquor A after being sterilized；
S2, by the NaOH solution tune pH value of solution of 0.09mol/l to 7.8-8.2 of the A in S1；
Compound protease is added in S3, and additive amount is the 0.05% of corn germ cake mixed liquor weight, slowly stirs at 30-50 DEG C of heat preservation
It mixes, mixing speed is that 15-22 turns/min, maintains 2h, is digested, obtain enzymolysis liquid B；
The enzymolysis liquid B that S4, S3 are obtained, is added alkali protease Promod523P liquid, and the every 10g enzymolysis liquid of additive amount adds 1.0-
1.5 μ L, enzymolysis liquid pH value is 8-9 at this time, 30-55 DEG C of temperature controlled water baths, constant temperature 40 minutes, carries out the dissolution of albumen, is formed
Protein dissolution liquid, is then added neutral proteinase in protein dissolution liquid, and additive amount is that 10g protein dissolution liquid adds 0.7-1.5 μ
L, pH value is 4.2-5.2 at this time, continues to be kept for 35-45 DEG C of temperature 15 minutes；
S5, will in S4 gained the enzyme deactivation in 100 DEG C of water-baths of protein dissolution liquid, is placed after enzyme deactivation ice water water-bath 1-2 minutes cooling
To 25 DEG C；
Protein dissolution liquid obtained by S5 is put into centrifuge centrifuge separation, centrifuge speed 4000r/min, centrifugation time by S6
10min takes clear liquid to use；
S7, clear liquid evaporate moisture by heating, obtain the sticky albumen powder of moisture content 18-22%, dry using 80 DEG C of baking ovens of temperature
Albumen peptide product is obtained after dry.
2. a kind of extracting method of corn protein peptide according to claim 1, which is characterized in that in the S1, described bar
Family name disinfection concrete operations be：At a temperature of corn germ cake mixed liquor is put into 68-70 DEG C heat 30min after rapid cooling extremely
4-5 DEG C, the corn germ cake mixed liquor after being sterilized.
3. a kind of extracting method of corn protein peptide according to claim 1, which is characterized in that, will be in S1 in the S2
The A NaOH solution tune pH value of solution of 0.09mol/l to 8.0.
4. a kind of extracting method of corn protein peptide according to claim 1, which is characterized in that in the S4, S3 is obtained
Enzymolysis liquid B, alkali protease Promod523P liquid is added, the every 10g enzymolysis liquid of additive amount adds 1.25 μ L, at this time enzymolysis liquid pH
Value is 8.5,42.5 DEG C of temperature controlled water baths, constant temperature 40 minutes, carries out the dissolution of albumen, protein dissolution liquid is formed, then in egg
Neutral proteinase is added in white lysate, additive amount is that 10g protein dissolution liquid adds 1.1 μ L, and pH value is 4.7 at this time, after continuation of insurance
It holds 40 DEG C of temperature 15 minutes.
5. a kind of extracting method of corn protein peptide according to claim 1, which is characterized in that in the S7, clear liquid warp
Crossing heating evaporates moisture, obtains the sticky albumen powder of moisture content 20%, produces using protein peptides are obtained after 80 DEG C of baking oven drying of temperature
Product.
6. a kind of extracting method of corn protein peptide according to claim 1, which is characterized in that S5, will in S4 gained
The enzyme deactivation in 100 DEG C of water-baths of protein dissolution liquid places ice water water-bath 2 minutes after enzyme deactivation and is cooled to 25 DEG C.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111321190A (en) * | 2020-03-26 | 2020-06-23 | 安徽顺鑫盛源生物食品有限公司 | Processing method of soybean protein peptide with pressure reduction function |
| CN112592951A (en) * | 2020-12-31 | 2021-04-02 | 新疆希普生物科技股份有限公司 | Corn protein peptide and extraction method thereof |
| CN112791103A (en) * | 2020-12-31 | 2021-05-14 | 四平华科生物技术有限责任公司 | Deer blood product and preparation method thereof |
-
2018
- 2018-08-01 CN CN201810860907.3A patent/CN108913743A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111321190A (en) * | 2020-03-26 | 2020-06-23 | 安徽顺鑫盛源生物食品有限公司 | Processing method of soybean protein peptide with pressure reduction function |
| CN112592951A (en) * | 2020-12-31 | 2021-04-02 | 新疆希普生物科技股份有限公司 | Corn protein peptide and extraction method thereof |
| CN112791103A (en) * | 2020-12-31 | 2021-05-14 | 四平华科生物技术有限责任公司 | Deer blood product and preparation method thereof |
| CN112592951B (en) * | 2020-12-31 | 2023-03-21 | 新疆希普生物科技股份有限公司 | Corn protein peptide and extraction method thereof |
| CN112791103B (en) * | 2020-12-31 | 2024-04-19 | 四平华科生物技术有限责任公司 | Deer blood product and preparation method thereof |
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