CN108913132B - Preparation method of dual-emission carbon-based nanoprobe and product thereof - Google Patents
Preparation method of dual-emission carbon-based nanoprobe and product thereof Download PDFInfo
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Abstract
The invention discloses a preparation method of a dual-emission carbon-based nano probe prepared by an electrostatic interaction induced self-assembly method and application of a product of the dual-emission carbon-based nano probe in temperature detection inside and outside an organism, and belongs to the technical field of colorimetric fluorescence detection. The invention comprises the synthesis of blue fluorescent carbon dots (B-CDs) which are not temperature sensitive and have negative charges on the surface, the preparation of red fluorescent carbon dots (R-CDs) which have temperature sensitivity and have positive charges on the surface and the preparation of a dual-emission carbon-based fluorescent nano probe. The dual-emission carbon-based fluorescence nanoprobe obtained by the invention has different responsivities to different temperatures by utilizing the dual-emission carbon-based fluorescence nanoprobe, presents different composite fluorescence color changes, can be used for naked eye colorimetric detection of the temperature inside and outside an organism, has high sensitivity (0.93%/° C) and operation repeatability, and provides convenience for aspects of biological imaging, temperature sensing, environmental monitoring, food safety and the like.
Description
Technical Field
The invention particularly relates to a preparation method of a dual-emission carbon-based nano material for temperature sensing and application of the dual-emission carbon-based nano material in detection of temperature change in a biological environment, and belongs to the technical field of fluorescent nano materials.
Background
The temperature plays a crucial role in physiological and biochemical actions and processes in organisms, and the accurate measurement of the temperature plays a significant role, so that the temperature measurement technology is promoted from the traditional invasive temperature sensors, namely thermocouples and thermistors, to non-invasive nano thermometers. Among them, the fluorescence nano thermometer has received wide attention due to its high temporal and spatial resolution and the integrated function of imaging and temperature sensing, and has been successfully applied to the aspects of bio-imaging, temperature sensing, environmental monitoring or food safety.
At present, commonly used fluorescent nano materials include semiconductor quantum dots, metal nanoclusters, carbon nano materials, rare earth doped nano particles and the like. Research has demonstrated the ability of AuNCs to detect changes in cell temperature. However, in complex environments, these single-emission fluorescence nanothermometers have poor stability and cannot accurately reflect changes in local temperature and temperature gradients in nano-or sub-nano regions. This inherent limitation has prompted the development of new fluorescent nanothermometers. Compared with the single-emission fluorescence nanometer thermometer, the sensitivity and the stability of the double-emission fluorescence nanometer thermometer are greatly improved due to the existence of the self-reference substance and the improvement of the material performance.
Carbon Dots (CDs) are a class of fluorescent nanomaterials without heavy metals, and have been widely used in many fields, especially in biological imaging and sensors, due to their inherent advantages, such as low cost, good biocompatibility, good stability, etc. To date, it has been reported that CDs-based fluorescence nanothermometers (single or dual emission) have been used for accurate temperature detection. For example, the CDs/Au NCs dual-emission fluorescent nano hybrid material can realize accurate measurement of physiological temperature in the range of 25-45 ℃. However, compatibility and interference between the two substances become the primary considerations for binary heterogeneous nanocomposites (CDs/AuNCs and CDs/QDs). The emergence of the double-carbon-point nano material not only solves the problems, but also further reduces the toxicity and the preparation cost of the nano material.
At present, the technologies for preparing dual carbon dots (CDs/CDs) nano materials mainly include chemical bond crosslinking and template-based self-assembly. However, these processes are complex and uncontrollable, ultimately leading to a decrease in quantum efficiency. Therefore, there is a need for developing a new temperature sensing material with low toxicity, stability, low cost, and simple preparation process.
Disclosure of Invention
The invention aims to construct a blue light-emitting carbon dot (B-CDs) and red light-emitting carbon dot (O-CDs) composite double-fluorescent carbon-based nano probe by a self-assembly method induced by electrostatic interaction, and the probe has the characteristics of stable structure, environmental friendliness, simple preparation process and the like, and can be used for temperature detection in organisms.
In order to realize the purpose of the invention, the invention provides a preparation method of a dual-emission carbon-based nanoprobe for temperature sensing, which comprises the following specific steps:
dissolving the red and blue fluorescent carbon dots according to the mass ratio of (1-3) to 1 to prepare aqueous solution, stirring for 3-5h, removing unassembled carbon dots, collecting the solution, and drying to obtain solid, namely the dual-emission carbon-based nano probe.
Further, the dissolution process is performed at room temperature.
Further, the drying mode is one of freeze drying, vacuum drying, reduced pressure boiling drying or normal pressure drying.
Furthermore, the drying mode is preferably freeze drying, and the drying temperature is-30 to-50 ℃.
Further, the drying time is 24-72 h.
Further, the mode for removing the unassembled carbon spots is dialysis, and the selected dialysis membrane is a 3500Da dialysis membrane.
Furthermore, the particle size distribution range of the obtained dual-emission carbon-based nano probe is 20-25 nm.
The preparation method of the red fluorescent carbon dot comprises the following steps:
preparing a carbon source solution, adding a nitrogen-containing compound, dissolving the mixture of the carbon source solution and the nitrogen-containing compound in an organic solvent, transferring the prepared solution to a reaction kettle, heating at the constant temperature of 160-200 ℃ for 6-10h, adding an alkaline solution, stirring, separating and removing supernatant, collecting precipitate, dissolving the precipitate in an acidic solution, stirring, separating and collecting precipitate, dissolving the precipitate in water, separating twice to remove precipitate, collecting supernatant, and drying to obtain the red fluorescent carbon dot.
Further, in the preparation process of the red fluorescent carbon dot, the molar ratio of the carbon source to the nitrogen-containing compound is 1: (5-10).
Further, in the preparation process of the red fluorescent carbon dot, the solvent is one or more than two of Dimethylformamide (DMF), dimethyl sulfoxide (DMSO) or methyl formamide.
Further, in the preparation process of the red fluorescent carbon dot, the alkaline solution is one of a sodium hydroxide solution or a potassium hydroxide solution, the concentration is 50mg/m L, and the volume is 40m L.
Further, in the preparation process of the red fluorescent carbon dot, the acid solution is one of a dilute hydrochloric acid solution, a dilute nitric acid solution or a dilute sulfuric acid solution, the concentration is 5%, and the volume is 50m L.
Further, in the preparation process of the red fluorescent carbon dots, the separation mode is centrifugal separation, wherein the centrifugal speed is 16000r/min, and the centrifugal time is 10 min.
Further, in the preparation process of the red fluorescent carbon dots, the drying mode is one of freeze drying, vacuum sealing drying or normal pressure drying.
Further, in the preparation process of the red fluorescent carbon dots, the carbon source is one or more than two of citric acid, p-phenylenediamine, terephthalic acid, o-phenylenediamine, m-phenylenediamine, melamine, glucose, fructose, sucrose, lactic acid, polyvinyl alcohol, polyacrylic acid, polyethyleneimine, chitosan, cellulose, lemon or shaddock peel.
Further, in the preparation process of the red fluorescent carbon dot, the carbon source is preferably citric acid, p-phenylenediamine, o-phenylenediamine, m-phenylenediamine or melamine.
Further, in the preparation process of the red fluorescent carbon dot, the concentration of the carbon source solution is 0.05-0.1g/m L.
Further, in the preparation process of the red fluorescent carbon dot, the nitrogen-containing compound is one or more than two of urea, thiourea, ethylenediamine, semicarbazide, triethylene tetramine, diphenylamino urea, tryptophan, glutathione, histidine, arginine, glycine or cysteine.
Further, in the preparation process of the red fluorescent carbon dot, the nitrogen-containing compound is preferably urea, thiourea, ethylenediamine or semicarbazide.
Furthermore, the particle size distribution range of the red fluorescent carbon dots is 3-5nm, the maximum emission wavelength is 580-590nm, the temperature response range is-20-120 ℃, and the temperature sensitivity is 0.87%/DEG C.
The preparation method of the blue fluorescent carbon dot comprises the following steps:
preparing a carbon source solution, adding a nitrogen-containing compound, transferring the mixture of the carbon source solution and the nitrogen-containing compound into a reaction kettle, and heating for 6-8h at constant temperature of 120-160 ℃; and taking out the product, filtering to remove large-particle precipitates, collecting supernatant, dialyzing for 72 hours by using a dialysis membrane, collecting dialyzed solution, and removing water solvent by rotary evaporation to obtain solid powder, namely blue fluorescent carbon dots (B-CDs).
Further, in the preparation process of the blue fluorescent carbon dots, a 0.22 μm filter membrane is used in the filtration mode, and the dialysis membrane is a 3500Da dialysis membrane.
Further, in the preparation process of the blue fluorescent carbon dot, the molar ratio of the carbon source to the nitrogen-containing compound is 1: (1-3).
Further, in the preparation process of the blue fluorescent carbon dot, the carbon source is one or more than two of citric acid, m-phenylenediamine, melamine, glucose, fructose, sucrose, lactic acid, polyvinyl alcohol, polyacrylic acid, polyethyleneimine, chitosan, cellulose, lemon or shaddock peel.
Further, in the preparation process of the blue fluorescent carbon dot, the carbon source is preferably citric acid, glucose, fructose or melamine.
Further, in the preparation process of the blue fluorescent carbon dot, the nitrogen-containing compound is one or more than two of urea, thiourea, ethylenediamine, semicarbazide, triethylene tetramine, diphenylamino urea, tryptophan, glutathione, histidine, arginine, glycine or cysteine.
Further, in the preparation process of the blue fluorescent carbon dot, the nitrogen-containing compound is preferably urea, thiourea, ethylenediamine or semicarbazide.
Further, in the preparation process of the blue fluorescent carbon dot, the concentration of the carbon source solution is 0.05-0.1g/m L.
The particle size distribution range of the blue fluorescent carbon dots (B-CDs) obtained by the steps is 1-3nm, and the maximum emission wavelength is 400-470 nm.
The double-emitting carbon-based nano probe prepared by the preparation method for the double-emitting carbon-based nano probe for temperature sensing is characterized in that the surface of a blue carbon dot is negatively charged, the surface of a red carbon dot is positively charged, and the two carbon dots can be spontaneously assembled to form double-carbon-dot nano particles through simple electrostatic interaction.
Further, the product has dual-emission properties, and emission peaks are respectively positioned at 440nm and 590 nm.
Further, the temperature sensitivity of the above product was 0.93%/deg.C.
Furthermore, the temperature response range of the product is-20-120 ℃.
The invention has the following beneficial technical effects:
1) the preparation method is simple and low in cost;
2) the particle sizes of the blue fluorescent carbon dots and the red fluorescent carbon dots are smaller and are in the range of 1-5 nm; the particle size distribution range of the dual-emission carbon-based nano probe is 20-25nm, the particle size of the product is uniform, and the product has good dispersibility;
3) the surface of the blue fluorescent carbon dot contains a large amount of hydroxyl and carboxyl, and is negatively charged, and the surface of the red fluorescent carbon dot contains a large amount of amino and is positively charged, so that the blue fluorescent carbon dot and the red fluorescent carbon dot can form stably existing dual-emission carbon-based nanoparticles in a simple electrostatic interaction induced self-assembly mode without adding a template or a carrier material;
4) the fluorescent material has strong dual-fluorescence emission, and the fluorescence quantum efficiency is 15.4%;
5) the double-emitting carbon-based nano temperature sensor can be applied to temperature detection, can be switched between a low temperature (15 ℃) and a high temperature (85 ℃) and has high repeatability (basically unchanged after being repeatedly used for 8 times) and sensitivity (0.93%/DEG C);
6) the product is used as a temperature sensing material, has a wide response range, obviously improves the sensitivity due to the introduction of a self-reference substance, has high repeated operability, and can be applied to non-contact detection of solution and intracellular temperature change.
Drawings
FIG. 1 is a TEM image of a red fluorescent carbon dot of example 1;
FIG. 2 is a TEM image of a blue fluorescent carbon dot of example 1;
FIG. 3 is a TEM image of the dual-emission carbon-based fluorescent nanomaterial of example 1;
FIG. 4 is a fluorescence spectrum of the product of example 1;
FIG. 5 is a graph showing fluorescence intensity of the temperature sensor of example 1 during temperature increase from 15 ℃ to 85 ℃;
FIG. 6 is a graph showing the reproducibility of the temperature sensor of example 1;
FIG. 7 is a graph showing fluorescence intensity during temperature increase from 15 ℃ to 85 ℃ of the temperature sensor of comparative example 1;
fig. 8 is a reproducibility test chart of the temperature sensor of comparative example 1.
Detailed Description
The invention is further described with reference to specific examples.
Transmission electron microscopy: tecnai GI F20U-TWIN transmission electron microscope (200KV accelerating voltage).
Fluorescence spectrometer, Horiba JobinYvon Fluoromax 4C-L (France) spectrophotometer
And (3) repeatability test: and controlling the temperature to be 15 ℃ by using an additional temperature sensing controller, measuring the fluorescence intensity of the double peaks of the nano probe, increasing the temperature to 85 ℃, measuring the fluorescence intensity of the double peaks of the nano probe, reducing the temperature to 15 ℃, measuring the fluorescence intensity, circularly repeating the temperature increase and reduction for 8 times, and processing the obtained data to obtain a repeatability test spectrogram.
Measuring an emission wavelength; measured using a fluorescence spectrometer.
And (3) particle size analysis: the analysis and statistics of the transmission electron microscope image can be obtained.
Example 1
Weighing 1g of citric acid and 2g of urea, dissolving in 20m of L N, N-Dimethylformamide (DMF), transferring the prepared solution into a reaction kettle, heating at a constant temperature of 200 ℃ for 8h to obtain a black viscous solution, adding 40m of L sodium hydroxide solution (50mg/m L) into the mixed solution, removing supernatant through centrifugation (16000r/min, 10min), collecting precipitate, dissolving in dilute hydrochloric acid solution (50m L, 5%), stirring for 10min, removing supernatant through centrifugation (16000r/min, 10min), collecting precipitate, dissolving in deionized water, centrifuging (16000r/min, 10min) twice to remove precipitate, collecting supernatant, freeze-drying at-35 ℃ for 72h to obtain red fluorescent carbon dots, re-dispersing the product in water, and obtaining a transmission electron microscope image of the product, wherein the particle size of the product is uniform, 3-5nm and has good dispersibility.
Weighing 1g of citric acid and 0.8 g of urea, dissolving in 10m L deionized water, transferring the solution into a reaction kettle, heating at a constant temperature of 120 ℃ for 8 hours to obtain a dark blue transparent solution, filtering the mixed solution by using a 0.22 mu m filter membrane to remove large particulate matters, dialyzing the filtered solution by using a 3500Da dialysis membrane, collecting dialysate, removing an aqueous solvent by rotary evaporation to obtain a product, and redispersing the product in water, wherein the transmission electron microscope picture of the product is shown in figure 2, the particle size of the product is uniform and is 1-3nm, and the product has good dispersibility.
Respectively weighing 10mg of red fluorescent carbon dot powder and 5mg of blue fluorescent carbon dot powder, dissolving the red fluorescent carbon dot powder and the blue fluorescent carbon dot powder in 5m L deionized water, stirring the solution at room temperature for 3 hours to ensure that two carbon dots are fully contacted, finally dialyzing the solution by using a 3500Da dialysis membrane, collecting the solution in a dialysis bag, freeze-drying the solution into powder to obtain a product, re-dispersing the product in an aqueous solution to form a temperature sensor with the concentration of 10mg/m L, and using a transmission electron microscope to show that the particle size of the product is uniform and is 15-20 nm.
The fluorescence property of the double-emission carbon-based material is tested by a fluorescence spectrometer, the result is shown in figure 4, the maximum emission wavelengths of the double-emission carbon-based material are respectively positioned at 590nm and 440nm and respectively correspond to the maximum emission wavelengths of a red fluorescence carbon dot and a blue fluorescence carbon dot, a 3m L aqueous solution of the double-emission carbon-based fluorescence nano material is taken to form a temperature sensor, a fluorescence spectrum instrument is used for testing the fluorescence intensity change of the double-emission carbon-based nano probe in the temperature rising process from 15 ℃ to 85 ℃, the sensitivity of the temperature is tested, the result is shown in figure 5, the average temperature change rate is-0.93%/° C, figure 6 is used for testing the stability of the temperature sensor, the product is repeatedly tested at 15 ℃ and 85 ℃, and the result shows that the temperature sensor can be switched between low temperature and high temperature, the corresponding fluorescence intensity at each temperature is basically kept unchanged, and the temperature can be repeatedly used for 8 times.
Example 2
Weighing 0.6g of p-phenylenediamine and 2g of urea, dissolving in 20m of L N, N-Dimethylformamide (DMF), transferring the prepared solution into a reaction kettle, heating at the constant temperature of 200 ℃ for 8 hours to obtain a black viscous solution, adding 40m of L potassium hydroxide solution (50mg/m L) into the mixed solution, removing supernatant through centrifugation (16000r/min and 10min), collecting precipitate, dissolving in a dilute nitric acid solution (50m L and 5%), stirring for 10 minutes, removing supernatant through centrifugation (16000r/min and 10min), collecting precipitate, dissolving in deionized water, centrifuging (16000r/min and 10min) twice to remove precipitate, collecting supernatant, and freeze-drying at-50 ℃ for 45 hours to obtain the red fluorescent carbon dot.
Weighing 1g of glucose and 0.5g of urea, dissolving in 10m L deionized water, transferring the solution into a reaction kettle, heating at the constant temperature of 120 ℃ for 8 hours to obtain a blue transparent solution, filtering the mixed solution by using a 0.22 mu m filter membrane to remove large particulate matters, dialyzing the filtered solution by using a 3500Da dialysis membrane, collecting dialysate, and removing a water solvent by rotary evaporation to obtain a product.
Weighing 6mg of red luminescent carbon dot powder and 2mg of blue luminescent carbon dot powder respectively, dissolving in 5m L deionized water, stirring the solution at room temperature for 3 hours so that the two carbon dots are fully contacted, finally dialyzing the solution by using a 3500Da dialysis membrane, collecting the solution in a dialysis bag, freeze-drying the solution into powder, namely obtaining a product, and re-dispersing the product in an aqueous solution to form the temperature sensor with the concentration of 10mg/m L.
Example 3
Weighing 0.5g of o-phenylenediamine and 2g of ethylenediamine, dissolving in 20m L of dimethyl sulfoxide (DMSO), transferring the prepared solution into a reaction kettle, heating at the constant temperature of 200 ℃ for 8 hours to obtain a black viscous solution, adding 40m L of sodium hydroxide solution (50mg/m L) into the mixed solution, removing supernatant through centrifugation (16000r/min, 10min), collecting precipitate, dissolving in dilute hydrochloric acid solution (50m L, 5%), stirring for 10min, removing supernatant through centrifugation (16000r/min, 10min), collecting precipitate, dissolving in deionized water, centrifuging (16000r/min, 10min) twice to remove precipitate, collecting supernatant, and performing vacuum drying to obtain the red fluorescent carbon dot.
Weighing 1g of fructose and 0.5g of urea, dissolving in 10m L deionized water, transferring the solution into a reaction kettle, heating at the constant temperature of 120 ℃ for 8 hours to obtain a blue-green transparent solution, filtering the mixed solution by using a 0.22 mu m filter membrane to remove large particulate matters, dialyzing the filtered solution by using a 3500Da dialysis membrane, collecting dialysate, and removing a water solvent by rotary evaporation to obtain a product.
Respectively weighing 10mg of red luminous carbon dot powder and 5mg of blue luminous carbon dot powder, dissolving the red luminous carbon dot powder and the blue luminous carbon dot powder in 5m L deionized water, stirring the solution at room temperature for 3 hours so that the two carbon dots are fully contacted, finally dialyzing the solution by using a 3500Da dialysis membrane, collecting the solution in a dialysis bag, freeze-drying the solution into powder, namely obtaining a product, and re-dispersing the product in an aqueous solution to form the temperature sensor with the concentration of 10mg/m L.
Comparative example 1: properties of the product obtained in the absence of a self-reference substance
Weighing 0.6g of p-phenylenediamine and 2g of urea, dissolving in 20m of L N, N-Dimethylformamide (DMF), transferring the prepared solution into a reaction kettle, heating at the constant temperature of 200 ℃ for 8 hours to obtain a black viscous solution, adding 40m of L sodium hydroxide solution (50mg/m L) into the mixed solution, removing supernatant through centrifugation (16000r/min, 10min), collecting precipitate, dissolving in dilute hydrochloric acid solution (50m L, 5%), stirring for 10min, removing supernatant through centrifugation (16000r/min, 10min), collecting precipitate, dissolving in deionized water, centrifuging (16000r/min, 10min) twice to remove precipitate, collecting supernatant, and freeze-drying at-50 ℃ for 35 hours to obtain the red fluorescent carbon dot.
50mg of red fluorescent carbon dot powder is weighed and dissolved in 5m L deionized water, then the solution is stirred for 3 hours at room temperature to form a temperature sensor with the concentration of 10mg/m L, the transmission electron microscope of the temperature sensor is shown in figure 1, and the particle size of the product is uniform and is 3-5 nm.
The fluorescence property of the single-emission carbon-based material is tested by a fluorescence spectrometer, the result is shown in fig. 4, the maximum emission wavelength of the single-emission carbon-based material is 590nm, 3m L of aqueous solution of the double-emission carbon-based fluorescent nano material is used for forming a temperature sensor, a fluorescence spectrometer is used for testing the change of the fluorescence intensity of the double-emission carbon-based nano probe in the temperature rising process from 15 ℃ to 85 ℃, the sensitivity of the temperature is tested, the result is shown in fig. 7, the average temperature change rate is-0.87%/° c, fig. 8 is used for testing the stability of the temperature sensor, the product is repeatedly tested at 15 ℃ and 85 ℃, and the result shows that compared with the temperature sensor in example 1, the stability of the temperature sensor is influenced by low/high temperature switching, the corresponding fluorescence intensities at different temperatures are changed, and the repeatability is poor.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (4)
1. A method for preparing a dual emission carbon-based nanoprobe for temperature sensing, the method comprising the steps of: dissolving the red and blue fluorescent carbon dots according to the mass ratio of (1-3) to (1) to prepare an aqueous solution, stirring for 3-5h, removing unassembled carbon dots, collecting the solution, and drying to obtain a solid, namely the dual-emission carbon-based nano probe, wherein the dual-emission carbon-based nano probe is obtained by self-assembling the red and blue fluorescent carbon dots through electrostatic interaction induction; wherein the content of the first and second substances,
the preparation method of the red fluorescent carbon dots comprises the following steps: preparing a carbon source solution, adding a nitrogen-containing compound, dissolving the mixture of the carbon source solution and the nitrogen-containing compound in an organic solvent, transferring the prepared solution into a reaction kettle, heating at the constant temperature of 160-200 ℃ for 6-10h, adding an alkaline solution, stirring, separating and removing a supernatant, collecting a precipitate, dissolving the precipitate in an acidic solution, stirring, separating and collecting the precipitate, dissolving the precipitate in water, separating twice to remove the precipitate, collecting the supernatant, and drying to obtain a red fluorescent carbon dot;
the preparation method of the blue fluorescent carbon dot comprises the following steps: preparing a carbon source solution, adding a nitrogen-containing compound, transferring the mixture of the carbon source solution and the nitrogen-containing compound into a reaction kettle, and heating for 6-8h at constant temperature of 120-160 ℃; taking out the product, filtering to remove large-particle precipitates, collecting supernatant, dialyzing for 72 hours by using a dialysis membrane, collecting dialyzed solution, and removing water solvent by rotary evaporation to obtain solid powder, namely the blue fluorescent carbon dots;
the carbon source used in the preparation process of the red fluorescent carbon dots is citric acid, p-phenylenediamine, o-phenylenediamine, m-phenylenediamine or melamine; the nitrogen-containing compound is urea, thiourea, ethylenediamine or semicarbazide; the organic solvent is one or more than two of dimethylformamide, dimethyl sulfoxide or methyl formamide; the alkaline solution is one of a sodium hydroxide solution or a potassium hydroxide solution; the acid solution is one of a dilute hydrochloric acid solution, a dilute nitric acid solution or a dilute sulfuric acid solution;
the carbon source used in the preparation process of the blue fluorescent carbon dots is citric acid, glucose, fructose or melamine; the nitrogen-containing compound is urea, thiourea, ethylenediamine or semicarbazide;
the molar ratio of the carbon source to the nitrogen-containing compound in the red fluorescent carbon dot preparation process is 1 (5-10), the molar ratio of the carbon source to the nitrogen-containing compound in the blue fluorescent carbon dot preparation process is 1 (1-3), and the concentration of the carbon source in the two carbon dot preparation processes is 0.05-0.1g/m L.
2. The method of claim 1, wherein the removing of the unassembled carbon spots is dialysis, and the drying is one of freeze drying, vacuum drying, boiling drying under reduced pressure, or drying under atmospheric pressure.
3. The dual emission carbon-based nanoprobe prepared by the method for preparing the dual emission carbon-based nanoprobe for temperature sensing according to claim 1 or 2.
4. Use of the dual emission carbon-based nanoprobe of claim 3 for temperature sensing.
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