CN108904883A - A kind of bone reparing biological material and preparation method thereof using abalone shells preparation - Google Patents
A kind of bone reparing biological material and preparation method thereof using abalone shells preparation Download PDFInfo
- Publication number
- CN108904883A CN108904883A CN201811158894.1A CN201811158894A CN108904883A CN 108904883 A CN108904883 A CN 108904883A CN 201811158894 A CN201811158894 A CN 201811158894A CN 108904883 A CN108904883 A CN 108904883A
- Authority
- CN
- China
- Prior art keywords
- bone
- preparation
- solution
- carboxymethyl chitosan
- collagen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/025—Other specific inorganic materials not covered by A61L27/04 - A61L27/12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Dermatology (AREA)
- General Health & Medical Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Veterinary Medicine (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Dispersion Chemistry (AREA)
- Materials For Medical Uses (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of bone reparing biological materials and preparation method thereof using abalone shells preparation, it uses natural polymer collagen, carboxymethyl chitosan, poly lactic-co-glycolic acid as organic substrate, abalone shells particle is as inorganic matrix, calcium source abundant is provided using its ingredient advantage, and provide multi-signal molecule, to promote the reparation of bone defect, bionical the bone impairment renovation material is made in conjunction with being freeze-dried through emulsion template method.Preparation process of the present invention is simple, reaction is mild, resulting materials combine organic and inorganic constituents and form imitative bone component, and freeze-drying is combined to form imitative bone structure by emulsion template method, the hole in material is set to be interconnected, mechanical performance and cell compatibility are good, have wide development and application prospect in bone tissue engineer field.
Description
Technical field
The invention belongs to field of compound material, and in particular to a kind of abalone shells bone reparing biological material and its bionical preparation side
Method.
Background technique
Bone is second of most common transplanting tissue in the world, and there are about 4,000,000 kinds of bone collections or bone substitute to be used for every year
The treatment of bone defect；However, the bone defect due to caused by wound, tumour, cancer, deformity, infection and arthritis, makes bone tissue
Treatment is seriously affected, also, the clinical demand of bone collection is still continuing growing.The appearance of bone tissue engineer is bone defect
Treatment provide possibility, key point is the successful preparation of biologic bracket material.
The porous support of conventional method synthesis is mainly prepared by materials such as metal, ceramics, is designed to natural bone group
Ingredient, structure and the property knitted consider insufficient.Thus, the bracket of these synthesis tends not to host's group with cell and surrounding
Good combination is knitted, and mechanical performance mismatches, surface microenvironment is unfriendly, and corrosion and abrasion, Bu Nengliang occur for the material of implantation
The bone tissue of repair deficiency well, so that therapeutic effect is undesirable.Therefore, the ingredient of bracket and structure are for bone tissue defect
It repairs of crucial importance.
Human body natural's bone is mainly collectively constituted by organic substrate and inorganic matrix on ingredient, branch of the organic substrate as bone
Frame assigns the good toughness of bone and elasticity and can account for about backbone weight with the crystallization and growth of Effective Regulation inorganic mineral
35%, including collagenous fiber beam, glycosaminoglycan and protein etc.；Inorganic matrix has bone centainly in conjunction with organic substrate
Hardness and brittleness, play the role of mechanical support, account for about the 65% of backbone weight, and main component is nanometer hydroxyapatite（Ca10
(PO4)6(OH)2, nHAP）Crystal grain.In addition, natural bone is a kind of material of three-dimensional compound porous structure, this structure can be thin
Growth, proliferation and the migration of born of the same parents provides effective space and carries out nutriment transport and metabolic waste discharge, is defective tissue
Repair and reconstruction provide mechanical support.Therefore, by by the benign combination of organic substrate and inorganic matrix（Ingredient imitates bone）And
Structure imitates bone, is expected to develop the biologic bracket material with more preferable performance.
The calcium carbonate that abalone shells are about 95% by volume fraction（CaCO3）Lamella composition, the biology through volume fraction about 5% are high
Molecule（Protein and polysaccharide etc.）Bonding, forms orderly organic-inorganic layered bionic structure, similar with nature bone, and abalone
Shell is from a wealth of sources, at low cost, with importantly, some researches show that, abalone shells can with synthesized human sclerous tissues it is main it is inorganic at
Divide hydroxyapatite, there is good bioactivity, biocompatibility and osteoconductive, and contain multi-signal in abalone shells
Molecule, such as bone morphogenetic protein can activate skeletonization bone marrow cell in vitro in vivo, promote osteoblastic proliferation and cell
Epimatrix（ECM）Generation and mineralising, this indicates that abalone shells have potential application in bone tissue engineer.
Collagen is the natural extracellular matrix of many body tissues（ECM）Ingredient is widely present in bone, skin, flesh
In the connective tissues such as tendon, account for about the 25-30% of total protein content；Meanwhile collagen participates in the Activities of cell, it can be thin
Under intracellular growth, differentiation, form generation and many pathological states, promoted by adjusting the dynamic interaction of cell and collagen
Into wound healing and regeneration；Also, collagen with its special structure, can be degradable biological property, it is extensive
Applied to bone tissue engineer.Carboxymethyl chitosan（CMCS）It is chitosan（CS）It is generated under alkaline condition with chloroacetate reaction
Product is a kind of polyampholyte.Carboxymethyl chitosan overcomes this poor disadvantage of dissolubility on the basis of chitosan,
It can completely dissolve in water, greatly promote, possess more in terms of antibacterial, antioxygenic property, biological degradability, biocompatibility
Good bioactivity, can speed up the generation of skeletonization.Poly lactic-co-glycolic acid（PLGA）It is polylactic acid（PLLA）With poly- hydroxyl second
Acid（PGA）It is modified by the methods of copolymerization, blending, plasticising to combine the advantage of the two performance, overcome respective bad
Gesture has good biocompatibility, biodegradability, can by adjusting the content of both polylactic acid and polyglycolic acid
To realize that polylactic-co-glycolic acid structural form, mechanical performance and degradation speed are controllable, catabolite can be converted into titanium dioxide
Carbon and water are excluded in vitro by body metabolism, and material has excellent processing performance, easy to form, in organizational project branch
The fields such as the preparation of frame generally use.
The present invention is bionical from ingredient and structure to prepare bone impairment renovation material, selected on ingredient water-soluble collagen and
Carboxymethyl chitosan, oil-soluble polylactic-co-glycolic acid select abalone shells particle as inorganic matrix as organic substrate, and
And emulsion template method combination Freeze Drying Technique is used, it is perfectly combined water-soluble organic substrate with oil-soluble organic group matter, makes
Obtain three-dimensional composite porous material.It utilizes collagen, the benign combination of carboxymethyl chitosan and polylactic-co-glycolic acid and inorganic base
The advantage of matter abalone shells particle components improves the physics and chemistry and bone formation performance of material, and being expected to, which becomes the novel treatment bone of one kind, lacks
The composite biological material of damage.
Summary of the invention
The bone reparing biological material and preparation method thereof that the purpose of the present invention is to provide a kind of to be prepared using abalone shells,
It is to realize by the capsomeric addition of abalone and the use of emulsion template method and construct shape in terms of ingredient imitates bone and structure imitates bone two
At the biomaterial for being suitable for Bone Defect Repari.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of bone reparing biological material prepared using abalone shells is using collagen, carboxymethyl chitosan, polylactic acid-
Hydroxyacetic acid combines through emulsion template method as inorganic matrix as organic substrate, abalone shells particle and is freeze-dried bionical preparation
The bone impairment renovation material；Wherein, abalone shells particle is also used as emulsifier, with promote water-soluble organic substrate collagen,
The mixing of carboxymethyl chitosan and oil-soluble organic group matter poly lactic-co-glycolic acid.
The abalone shells particle is to be sieved to be made through 200 mesh screens after crushing abalone shells.
The preparation method of the bone reparing biological material includes the following steps：
（1）Collagen is dissolved in deionized water, magnetic agitation 12 hours, it is dissolved sufficiently, obtains 2.45wt%-9.8wt%
Collagen solution；
（2）Carboxymethyl chitosan is dissolved in deionized water, magnetic agitation 2-3 hours, dissolves it sufficiently, obtains the carboxylic first of 2wt%
Base chitosan solution；
（3）By step（1）Gained collagen solution and step（2）Gained carboxymethyl chitosan solution by volume 1:1 mixing,
Aqueous phase solution is made；
（4）Abalone shells particle is added to step（3）In gained aqueous phase solution, ultrasonic 5-10min is configured to 0.75wt%-
The particle dispersion mixing liquid of 3.0wt%；
（5）Poly lactic-co-glycolic acid is dissolved in methylene chloride, the oil-phase solution of 4wt% is prepared into；
（6）By particle dispersion mixing liquid and oil-phase solution by volume 5:2 are sufficiently mixed, and crosslinking agent is added and is fixed；
（7）By step（6）Gained mixed solution is at room temperature with 15 × 103The revolving speed of rpm emulsifies 1min, be then transferred to rapidly-
80 DEG C are pre-chilled 12 hours, and subsequent vacuum freeze drying 3d obtains the bone reparing biological material.
Step（6）Described in crosslinking agent be glutaraldehyde, additional amount is by contained aldehyde radical and carboxymethyl chitosan in glutaraldehyde
Molar ratio with amino total amount contained in collagen is 1:1 converts.
Remarkable advantage of the invention is：
（1）On raw material, the present invention combines synthesis macromolecule using natural polymer, overcomes the performance of single type material
Disadvantage adequately achieves the performance complement of every kind of material；
（2）On ingredient, the present invention selects organic substrate to combine with inorganic matrix, the bionical building biology material from bone component
Material makes resulting materials have the advantage of class bone composition；And under the induction of organic substrate, inorganic matrix is easier to crystallization life
Long, bioactivity is improved, and interface binding power and mechanical strength are improved；
（3）In structure, the present invention uses abalone shells particle（AS）Instead of conventional surfactant, obtained by emulsion template method
Imitative bone three-dimensional porous structure, constructs bone defect healing biomaterial；And emulsion template method can effectively have water solubility
Machine matrix with oil-soluble organic group matter is uniform mixes, overcome water soluble polymer and oil-soluble Polymer Solution be immiscible
Defect；
（4）By changing the amount of collagen, carboxymethyl chitosan, poly lactic-co-glycolic acid and crosslinking agent glutaraldehyde, can synthesize suitable
Biomaterial needed for symphysis object different parts, and by control polylactic acid and the ratio of polyglycolic acid, it can be achieved that poly-
The Properties Control of lactic-co-glycolic acid；
（5）Biomaterial prepared by the present invention has the porous structure being intertwined, and its satisfactory mechanical property, biological safety
Height, and have can plastic property, preparation condition is mild, simple process and low cost, and material is safe and non-toxic.
Detailed description of the invention
Fig. 1 is scanning electron microscope sem figure of the 2 gained Bone Defect Repari biologic bracket material of embodiment under different enlargement ratios,
In,（a）200 ×,（b）500 ×,（c）10000×；
Fig. 2 is scanning electron microscope sem figure of the 2 gained Bone Defect Repari biologic bracket material of comparative example under different enlargement ratios, wherein
（a）200 ×,（b）500 ×,（c）10000×；
Fig. 3 is the porosity comparative situation figure of Bone Defect Repari biologic bracket material obtained by embodiment and comparative example；
Fig. 4 is that the mechanical performance of Bone Defect Repari biologic bracket material obtained by embodiment and comparative example compares；
Fig. 5 is Bone Defect Repari biologic bracket material obtained by embodiment and comparative example and the cell Proliferation after osteoblast compound criteria
Rate.
Specific embodiment
In order to make content of the present invention easily facilitate understanding, With reference to embodiment to of the present invention
Technical solution is described further, but the present invention is not limited only to this.
Embodiment 1
（1）It takes 1.225g collagen to be dissolved in deionized water, magnetic agitation 12 hours, dissolves it sufficiently, be formulated as
The collagen solution of 2.45wt%；
（2）It takes 0.3g carboxymethyl chitosan to be dissolved in deionized water, 2-3 hour of magnetic agitation, dissolves it sufficiently, be configured to
The carboxymethyl chitosan sugar aqueous solution of 2wt%, as aqueous phase solution；
（3）By step（1）Gained collagen solution and step（2）Gained carboxymethyl chitosan solution by volume 1:1 mixing,
Aqueous phase solution is made（Continuous phase）；
（4）0.1125g abalone shells particle is taken, is added into above-mentioned aqueous phase solution, ultrasonic 5-10min is configured to 0.75wt%'s
Particle dispersion mixing liquid, as continuous phase；
（5）It takes 0.8g poly lactic-co-glycolic acid to be dissolved in 20mL methylene chloride, is prepared into the poly lactic-co-glycolic acid of 4wt%
Solution is also dispersed phase when identical as oil；
（6）With continuous phase and disperse phase volume than 5:2 ratio is mixed, and appropriate crosslinking agent glutaraldehyde is added thereto
（The amount of glutaraldehyde is added by mole of contained amino total amount in contained aldehyde radical in glutaraldehyde and carboxymethyl chitosan and collagen
Than being 1:1 converts）, with the macromolecular fixed in lotion continuous phase；
（7）By above-mentioned mixed liquor 15 × 10 in high speed disperser3Rpm emulsifies 1min, is then transferred to -80 DEG C of pre-coolings 12 rapidly
Hour, then by freeze drier vacuum freeze drying 3d, Bone Defect Repari biomaterial is prepared, is designated as C+S1.
Embodiment 2
（1）It takes 1.225g collagen to be dissolved in deionized water, magnetic agitation 12 hours, dissolves it sufficiently, be formulated as
The collagen solution of 2.45wt%；
（2）It takes 0.3g carboxymethyl chitosan to be dissolved in deionized water, 2-3 hour of magnetic agitation, dissolves it sufficiently, be configured to
The carboxymethyl chitosan sugar aqueous solution of 2wt%, as aqueous phase solution；
（3）By step（1）Gained collagen solution and step（2）Gained carboxymethyl chitosan solution by volume 1:1 mixing,
Aqueous phase solution is made（Continuous phase）；
（4）0.45g abalone shells particle is taken, is added into above-mentioned aqueous phase solution, ultrasonic 5-10min is configured to the grain of 3.0wt%
Sub- dispersion mixing liquid, as continuous phase；
（5）It takes 0.8g poly lactic-co-glycolic acid to be dissolved in 20mL methylene chloride, is prepared into the poly lactic-co-glycolic acid of 4wt%
Solution is also dispersed phase while as oily phase；
（6）With continuous phase and disperse phase volume than 5:2 ratio is mixed, and appropriate crosslinking agent glutaraldehyde is added thereto
（The amount of glutaraldehyde is added by mole of contained amino total amount in contained aldehyde radical in glutaraldehyde and carboxymethyl chitosan and collagen
Than being 1:1 converts）, with the macromolecular fixed in lotion continuous phase；
（7）By above-mentioned mixed liquor 15 × 10 in high speed disperser3Rpm emulsifies 1min, is then transferred to -80 DEG C of pre-coolings 12 rapidly
Hour, then by freeze drier vacuum freeze drying 3d, Bone Defect Repari biomaterial is prepared, is designated as C+S3.
Comparative example 1
（1）It takes 0.3g carboxymethyl chitosan to be dissolved in deionized water, 2-3 hour of magnetic agitation, dissolves it sufficiently, be configured to
The carboxymethyl chitosan sugar aqueous solution of 2wt%, as aqueous phase solution；
（2）0.1125g abalone shells particle is taken, is added into above-mentioned carboxymethyl chitosan sugar aqueous solution, ultrasonic 5-10min, is prepared
At the particle dispersion mixing liquid of 0.75wt%, as continuous phase；
（3）It takes 0.8g poly lactic-co-glycolic acid to be dissolved in 20mL methylene chloride, is prepared into the poly lactic-co-glycolic acid of 4wt%
Solution is also dispersed phase when identical as oil；
（4）With continuous phase and disperse phase volume than 5:2 ratio is mixed, and appropriate crosslinking agent glutaraldehyde is added thereto
（It is 1 that the amount that glutaraldehyde is added, which presses contained aldehyde radical and the amino-containing molar ratio of institute in carboxymethyl chitosan in glutaraldehyde,:1 is changed
It calculates）, with the macromolecular fixed in lotion continuous phase；
（5）By above-mentioned mixed liquor 15 × 10 in high speed disperser3Rpm emulsifies 1min, is then transferred to -80 DEG C of pre-coolings 12 rapidly
Hour, then by freeze drier vacuum freeze drying 3d, Bone Defect Repari biomaterial is prepared, is designated as S1.
Comparative example 2
（1）It takes 0.3g carboxymethyl chitosan to be dissolved in deionized water, 2-3 hour of magnetic agitation, dissolves it sufficiently, be configured to
The carboxymethyl chitosan sugar aqueous solution of 2wt%, as aqueous phase solution；
（2）0.45g abalone shells particle is taken, is added into above-mentioned carboxymethyl chitosan sugar aqueous solution, ultrasonic 5-10min is configured to
The particle dispersion mixing liquid of 3.0wt%, as continuous phase；
（3）It takes 0.8g poly lactic-co-glycolic acid to be dissolved in 20mL methylene chloride, is prepared into the poly lactic-co-glycolic acid of 4wt%
Solution is also dispersed phase when identical as oil；
（4）With continuous phase and disperse phase volume than 5:2 ratio is mixed, and appropriate crosslinking agent glutaraldehyde is added thereto
（It is 1 that the amount that glutaraldehyde is added, which presses contained aldehyde radical and the amino-containing molar ratio of institute in carboxymethyl chitosan in glutaraldehyde,:1 is changed
It calculates）, with the macromolecular fixed in lotion continuous phase；
（5）By above-mentioned mixed liquor 15 × 10 in high speed disperser3Rpm emulsifies 1min, is then transferred to -80 DEG C of pre-coolings 12 rapidly
Hour, then by freeze drier vacuum freeze drying 3d, Bone Defect Repari biomaterial is prepared, is designated as S3.
Fig. 1,2 are respectively embodiment 2 and 2 gained Bone Defect Repari biologic bracket material sweeping under different enlargement ratios of comparative example
Retouch Electronic Speculum SEM figure.It can be seen that two kinds of materials all have interconnected hole and complete structure, but 10000 × times
Under rate, abalone shells particle is high-visible in Bone Defect Repari biologic bracket material obtained by comparative example, and the biology of Bone Defect Repari obtained by embodiment
Only visible a small amount of abalone shells particle in timbering material, illustrates the addition of collagen so that abalone shell particles are more completely fitted into
In organic substrate.
Fig. 3 is the porosity comparative situation figure of Bone Defect Repari biologic bracket material obtained by embodiment and comparative example.By can in figure
See, due to the addition of collagen, the percentage that organic substrate accounts for entire matrix increases, and makes the resulting Bone Defect Repari biology of embodiment
The hole of timbering material becomes larger.
Fig. 4 is that the mechanical performance of Bone Defect Repari biologic bracket material obtained by embodiment and comparative example compares.It can be seen that plus
Enter collagen, inorganic matrix is closer in conjunction with organic substrate, so as to make the machine of Bone Defect Repari biologic bracket material obtained by embodiment
Tool performance boost.
Fig. 5 is that Bone Defect Repari biologic bracket material obtained by embodiment and comparative example and the cell after osteoblast compound criteria increase
Grow rate.It can be seen that the cell compatibility of Bone Defect Repari biologic bracket material obtained by embodiment is preferable compared with comparative example, this
Mainly due to the organic substrate that collagen is natural bone tissue, more advantage on ingredient, therefore the cell phase of material can be improved
Capacitive.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (6)
1. a kind of bone reparing biological material prepared using abalone shells, it is characterised in that：Using collagen, carboxymethyl chitosan
Sugar, poly lactic-co-glycolic acid are as organic substrate, and abalone shells particle is as inorganic matrix and emulsifier, through emulsion template method knot
Conjunction freeze-drying is bionical to prepare the bone impairment renovation material；
The abalone shells particle is to be sieved to be made through 200 mesh screens after crushing abalone shells.
2. a kind of preparation method of bone reparing biological material as described in claim 1, it is characterised in that：Include the following steps：
（1）Collagen is dissolved in deionized water, magnetic agitation 12 hours, it is dissolved sufficiently, obtains collagen solution；
（2）Carboxymethyl chitosan is dissolved in deionized water, magnetic agitation 2-3 hours, it is dissolved sufficiently, obtains carboxymethyl chitosan
Sugar juice；
（3）By step（1）Gained collagen solution and step（2）Gained carboxymethyl chitosan solution by volume 1:1 mixing,
Aqueous phase solution is made；
（4）Abalone shells particle is added to step（3）In gained aqueous phase solution, ultrasonic 5-10min is configured to 0.75wt%-
The particle dispersion mixing liquid of 3.0wt%；
（5）Poly lactic-co-glycolic acid is dissolved in methylene chloride, the oil-phase solution of 4wt% is prepared into；
（6）Particle dispersion mixing liquid is sufficiently mixed in proportion with oil-phase solution, and crosslinking agent is added and is fixed；
（7）By step（6）Gained mixed solution is at room temperature with 15 × 103The revolving speed of rpm emulsifies 1min, be then transferred to rapidly-
80 DEG C are pre-chilled 12 hours, and subsequent vacuum freeze drying 3d obtains the bone reparing biological material.
3. preparation method according to claim 2, it is characterised in that：Step（1）The concentration of middle gained collagen solution
For 2.45wt% -9.8wt%.
4. preparation method according to claim 2, it is characterised in that：Step（2）Middle gained carboxymethyl chitosan solution
Concentration is 2wt%.
5. preparation method according to claim 2, it is characterised in that：Step（6）Middle particle dispersion mixing liquid mixes with oil
The volume ratio of liquid mixing is 5:2.
6. preparation method according to claim 2, it is characterised in that：Step（6）Described in crosslinking agent be glutaraldehyde, add
Entering amount by the molar ratio of contained amino total amount in aldehyde radical contained in glutaraldehyde and carboxymethyl chitosan and collagen is 1:1 carries out
Conversion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811158894.1A CN108904883A (en) | 2018-09-30 | 2018-09-30 | A kind of bone reparing biological material and preparation method thereof using abalone shells preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811158894.1A CN108904883A (en) | 2018-09-30 | 2018-09-30 | A kind of bone reparing biological material and preparation method thereof using abalone shells preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108904883A true CN108904883A (en) | 2018-11-30 |
Family
ID=64409982
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811158894.1A Pending CN108904883A (en) | 2018-09-30 | 2018-09-30 | A kind of bone reparing biological material and preparation method thereof using abalone shells preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108904883A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113368310A (en) * | 2021-06-22 | 2021-09-10 | 山东大学 | Preparation method of abalone shell particle micro-nano hybrid bone repair scaffold |
| CN114425103A (en) * | 2022-04-06 | 2022-05-03 | 中国科学院苏州纳米技术与纳米仿生研究所 | Bionic biogel and preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101125223A (en) * | 2007-09-27 | 2008-02-20 | 天津大学 | Method for preparing calcium phosphate cement/chitosan-gelatine composite porous holder |
| WO2008107484A3 (en) * | 2007-03-07 | 2009-09-03 | Coloplast A/S | Fistula plug comprising ecm |
| CN101856515A (en) * | 2010-06-04 | 2010-10-13 | 西南大学 | Method for preparing artificial bone from chitosan and shell powder serving as raw materials |
| CN104707179A (en) * | 2015-03-26 | 2015-06-17 | 福州大学 | Oil-soluble/water-soluble organic-inorganic three-phase porous micro-nanometer composite bone repair material |
| KR20160131584A (en) * | 2015-05-08 | 2016-11-16 | 조선대학교산학협력단 | Biomaterials using abalone shell and method thereof |
| CN107362392A (en) * | 2017-07-18 | 2017-11-21 | 福州大学 | A kind of Nano-hydroxyapatite/Carboxymethyl Chitosan/polylactic-co-glycolic acid micro-nano hydridization carried stent and its bionics method for preparation |
-
2018
- 2018-09-30 CN CN201811158894.1A patent/CN108904883A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008107484A3 (en) * | 2007-03-07 | 2009-09-03 | Coloplast A/S | Fistula plug comprising ecm |
| CN101125223A (en) * | 2007-09-27 | 2008-02-20 | 天津大学 | Method for preparing calcium phosphate cement/chitosan-gelatine composite porous holder |
| CN101856515A (en) * | 2010-06-04 | 2010-10-13 | 西南大学 | Method for preparing artificial bone from chitosan and shell powder serving as raw materials |
| CN104707179A (en) * | 2015-03-26 | 2015-06-17 | 福州大学 | Oil-soluble/water-soluble organic-inorganic three-phase porous micro-nanometer composite bone repair material |
| KR20160131584A (en) * | 2015-05-08 | 2016-11-16 | 조선대학교산학협력단 | Biomaterials using abalone shell and method thereof |
| CN107362392A (en) * | 2017-07-18 | 2017-11-21 | 福州大学 | A kind of Nano-hydroxyapatite/Carboxymethyl Chitosan/polylactic-co-glycolic acid micro-nano hydridization carried stent and its bionics method for preparation |
Non-Patent Citations (1)
| Title |
|---|
| CHEN TAIYING: "Preparation and charactorization of alginate/HACC/oyster shell powder biocomposite scaffolds for potential bone tissue engineering application", 《RSC ADVANCES》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113368310A (en) * | 2021-06-22 | 2021-09-10 | 山东大学 | Preparation method of abalone shell particle micro-nano hybrid bone repair scaffold |
| CN114425103A (en) * | 2022-04-06 | 2022-05-03 | 中国科学院苏州纳米技术与纳米仿生研究所 | Bionic biogel and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ige et al. | Natural products: a minefield of biomaterials | |
| Torgbo et al. | Bacterial cellulose-based scaffold materials for bone tissue engineering | |
| Zhang et al. | Stem cell-friendly scaffold biomaterials: applications for bone tissue engineering and regenerative medicine | |
| Shaheen et al. | Effect of cellulose nanocrystals on scaffolds comprising chitosan, alginate and hydroxyapatite for bone tissue engineering | |
| Yuan et al. | A novel bovine serum albumin and sodium alginate hydrogel scaffold doped with hydroxyapatite nanowires for cartilage defects repair | |
| Xing et al. | Chitin-hydroxyapatite-collagen composite scaffolds for bone regeneration | |
| Sah et al. | Soluble eggshell membrane: a natural protein to improve the properties of biomaterials used for tissue engineering applications | |
| Muthukumar et al. | Collagen/chitosan porous bone tissue engineering composite scaffold incorporated with Ginseng compound K | |
| Li et al. | Collagen-based implants reinforced by chitin fibres in a goat shank bone defect model | |
| Thomas et al. | Polymer composites, biocomposites | |
| Lalzawmliana et al. | Marine organisms as a source of natural matrix for bone tissue engineering | |
| Chuysinuan et al. | Injectable eggshell-derived hydroxyapatite-incorporated fibroin-alginate composite hydrogel for bone tissue engineering | |
| CN107737367A (en) | The vertebrae minimally-invasive of the bone cement combined using calcium phosphate is treated | |
| JP2008513100A (en) | Porous biomaterial-filler composite and process for producing the same | |
| Munhoz et al. | Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats | |
| CN101098718A (en) | Chitosan compositions | |
| JP2012512684A5 (en) | ||
| CN100381181C (en) | Step-degrdeable bioactive implanting material comprising growth promotion factor | |
| Chicatun et al. | Collagen/chitosan composite scaffolds for bone and cartilage tissue engineering | |
| Cortizo et al. | Biodegradable polymers for bone tissue engineering | |
| Chen et al. | Mineralized self-assembled silk fibroin/cellulose interpenetrating network aerogel for bone tissue engineering | |
| CN108904883A (en) | A kind of bone reparing biological material and preparation method thereof using abalone shells preparation | |
| CN102085389A (en) | Preparation method of injectable bone repair material | |
| Khiabani et al. | A review of hydrogel systems based on poly (N-isopropyl acrylamide) for use in the engineering of bone tissues | |
| Wang et al. | Sustainable aquaculture side-streams derived hybrid biocomposite for bone tissue engineering |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181130 |
|
| RJ01 | Rejection of invention patent application after publication |