CN108904794A - The immunization method of live vaccines of hog cholera - Google Patents
The immunization method of live vaccines of hog cholera Download PDFInfo
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- CN108904794A CN108904794A CN201811146800.9A CN201811146800A CN108904794A CN 108904794 A CN108904794 A CN 108904794A CN 201811146800 A CN201811146800 A CN 201811146800A CN 108904794 A CN108904794 A CN 108904794A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5254—Virus avirulent or attenuated
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
- A61K2039/541—Mucosal route
- A61K2039/542—Mucosal route oral/gastrointestinal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
- A61K2039/552—Veterinary vaccine
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24311—Pestivirus, e.g. bovine viral diarrhea virus
- C12N2770/24334—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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Abstract
The invention belongs to animal doctor's technical field of epidemic prevention, disclose a kind of immunization method of live vaccines of hog cholera, include the following steps：A. piglet group to be immunized is cut off the water supply 2 ~ 4 hours, the purified water for measuring piglet group's daily drink amount 20% ~ 40% to be immunized is put into container, again according to the volume of purified water, 1 ~ 4g/L of food grade saleratus, 1 ~ 2g/L of skimmed milk power, 4 ~ 7g/L of oral glucose are added in a reservoir, are mixed；B. piglet group's progress live vaccines of hog cholera head is exempted from or two exempts from, the live vaccines of hog cholera of suitable RID dosage is added to the container, is mixed；C. mixture in container is poured into swinery drinks tank, is freely drunk for piglet group to be immunized, drinks and finish in 1 ~ 2 hour, observed after immunization piglet group 1h is disposed in time to occurring anaphylactoid piglet individually.Immunization method provided by the invention is time saving, laborsaving, reduces group's stress reaction caused by conventional live vaccines of hog cholera injecting immune, improves Immune efficiency, reduce epidemic prevention worker's working strength.
Description
Technical field
The invention belongs to animal doctor's technical field of epidemic prevention, are related to a kind of immunization method of live vaccines of hog cholera.
Background technique
Swine fever is the important epidemic disease for endangering pig breeding industry, is the most effective means of the prevention and control disease using Attenuate vaccine.It uses at present
Attenuate vaccine mostly use the mode of intramuscular injection immune, using when live vaccines of hog cholera head of the method to piglet group exempts from, two exempt from, usually
It needs first to concentrate swinery, time-consuming and laborious by being only immunized, situations such as human factor easily causes leakage to exempt from, excessive immune generation, to pig
Stress be strong caused by group, immune opportunity is improper to be also easy to be influenced by maternal antibody, influences immune effect.
Vaccine has irritation small by the way that alimentary canal is immune, and work efficiency is high, is suitble to group's application, and good immune effect etc. is excellent
Gesture.Swine fever is mostly to infect (having eaten contaminated feed or drinking-water) by alimentary canal, first in tonsillotome in the natural environment
It is proliferated, enters each target organ and tissue through blood after 16~18h, lead to epidemic disease occurrence and development.But have not yet to see epidemic disease living
Seedling through alimentary canal immunization method swinery application.
Summary of the invention
It is time saving the purpose of the present invention is to provide a kind of immunization method of live vaccines of hog cholera, it is laborsaving, it is living to reduce conventional swine fever
Group's stress reaction, improves Immune efficiency, reduces epidemic prevention worker's working strength caused by vaccine injection is immune.
To achieve the above object, the present invention uses following technical scheme：
A kind of immunization method of live vaccines of hog cholera, includes the following steps：
A. piglet group to be immunized is cut off the water supply 2~4 hours, measures the purifying of piglet group's daily drink amount 20%~40% to be immunized
Water is put into container, then according to purified water volume, in a reservoir add 1~4g/L of food grade saleratus, skimmed milk power 1~
2g/L, 4~7g/L of oral glucose are mixed；
B. piglet group's progress live vaccines of hog cholera head is exempted from or two exempts from, appearance will be added after the live vaccines of hog cholera dilution of suitable RID dosage
In device, mix；
C. mixture in container is poured into piglet group's drinks tank, freely drinks for piglet group to be immunized, is drunk in 1~2 hour
With finishing, observed after immunization piglet group 1h is disposed in time to occurring anaphylactoid piglet individually.
Further, step b is specially：Piglet group's progress live vaccines of hog cholera head is exempted from or two exempt from, by every pig 750-
It is added to the container, mixes after the live vaccines of hog cholera dilution of 1500RID dosage.
Further, piglet is health pig in the piglet group to be immunized.
Compared with prior art, the beneficial effects of the present invention are：
1. the immunization method of live vaccines of hog cholera provided by the invention, simple to operation, without driving pig, pig is grabbed, it is time saving, it is laborsaving,
Group's stress reaction caused by conventional live vaccines of hog cholera injecting immune is reduced, Immune efficiency is improved, reduces epidemic prevention worker's work
Make intensity.
2. adding sodium bicarbonate in immunization method of the present invention to be conducive to neutralize gastric acid, the loss of vaccine vigor is reduced；Addition is de-
Rouge milk powder, oral glucose are conducive to protect the vigor of live vaccines of hog cholera, improve mouthfeel, and palatability is drunk in raising, compared to normal
Immunization method is advised, antibody generates level without significant difference, and the live vaccines of hog cholera for being suitable for Compact Develop is immune, is equally applicable
In the Swinery immunity of free-ranging.
Specific embodiment
The following examples are intended to illustrate the invention, but is not used to limit the scope of protection of the present invention.Unless otherwise specified, real
Apply the conventional means that technological means used in example is well known to those skilled in the art.Test method in following embodiments, such as
It is conventional method without special instruction.
The live vaccines of hog cholera used in following embodiments is the swine fever epidemic disease living of Shangqiu methylene blue bioengineering Co., Ltd production
Seedling, 10 part/bottles, every part 3000RID.
40 health wean ternary pigs are chosen, head is carried out when 35 age in days and is exempted from (weight is 6~8kg), when 70 age in days carries out two
Exempt from (22~28kg of weight), be divided into four groups, every group 10, first three groups use immunization method of the present invention, and detailed process is shown in embodiment 1
~3；4th group as a control group, and using musculi colli injecting immune, immunizing dose is every pig 1500RID.
Embodiment 1
It is 24 DEG C that first group of 10 piglet head, which exempts from environment temperature, and air humidity 58% is fine, and amount of drinking water is about 10000ml/
It.The immunization method of live vaccines of hog cholera, includes the following steps：
A1. piglet group to be immunized is cut off the water supply 2 hours, measures 2000ml purified water and be put into clean plastic containers, then in container
Middle addition food grade saleratus 2g, skimmed milk power 2g, oral glucose 8g are mixed；
B1. live vaccines of hog cholera head is carried out to piglet group to exempt from, after 1 bottle of live vaccines of hog cholera of 10ml diluted, take out 5ml (i.e.
Every pig 1500RID) it is added to the container, it mixes；
C1. mixture in container is poured into swinery drinks tank, freely drinks for piglet group to be immunized, has been drunk in 1 hour
Finish, observed after immunization piglet group 1h, no allergic reaction occurs.
Two exempt from environment temperature be 32 DEG C, air humidity 44% is fine, and amount of drinking water is about 30000ml/ days.Swine fever is living
The immunization method of vaccine, includes the following steps：
A2. piglet group to be immunized is cut off the water supply 2 hours, measures 6000ml purified water and be put into clean plastic containers, then in container
Middle addition food grade saleratus 24g, skimmed milk power 12g, oral glucose 42g are mixed；
B2. live vaccines of hog cholera two is carried out to piglet group to exempt from, after 1 bottle of live vaccines of hog cholera of 10ml diluted, take out 5ml (i.e.
Every pig 1500RID) it is added to the container, it mixes；
C2. mixture in container is poured into swinery drinks tank, freely drinks for piglet group to be immunized, is drunk in 1.5 hours
It finishes, observed after immunization piglet group 1h, no allergic reaction occurs.
Embodiment 2
It is 24 DEG C that second group of 10 piglet head, which exempts from environment temperature, and air humidity 58% is fine, and amount of drinking water is about 10000ml/
It.The immunization method of live vaccines of hog cholera, includes the following steps：
A1. piglet group to be immunized is cut off the water supply 2 hours, measures 3000ml purified water and be put into clean plastic containers, then in container
Middle addition food grade saleratus 7.5g, skimmed milk power 4.5g, oral glucose 16.5g are mixed；
B1. live vaccines of hog cholera head is carried out to piglet group to exempt from, after 1 bottle of live vaccines of hog cholera of 10ml diluted, take out 2.5ml
(i.e. every pig 750RID) is added to the container, and is mixed；
C1. mixture in container is poured into swinery drinks tank, freely drinks for piglet group to be immunized, has been drunk in 1 hour
Finish, observed after immunization piglet group 1h, no allergic reaction occurs.
Two exempt from environment temperature be 32 DEG C, air humidity 44% is fine, and amount of drinking water is about 30000ml/ days.Swine fever is living
The immunization method of vaccine, includes the following steps：
A2. piglet group to be immunized is cut off the water supply 2 hours, measures 9000ml purified water and be put into clean plastic containers, then in container
Middle addition food grade saleratus 27g, skimmed milk power 14g, oral glucose 55g are mixed；
B2. live vaccines of hog cholera two is carried out to piglet group to exempt from, after 1 bottle of live vaccines of hog cholera of 10ml diluted, take out 2.5ml
(i.e. every pig 750RID) is added to the container, and is mixed；
C2. mixture in container is poured into swinery drinks tank, freely drinks for piglet group to be immunized, is drunk in 1.5 hours
It finishes, observed after immunization piglet group 1h, no allergic reaction occurs.
Embodiment 3
It is 24 DEG C that 10 piglet head of third group, which exempt from environment temperature, and air humidity 58% is fine, and amount of drinking water is about 10000ml/
It.The immunization method of live vaccines of hog cholera, includes the following steps：
A1. piglet group to be immunized is cut off the water supply 4 hours, measures 4000ml purified water and be put into clean plastic containers, then in container
Middle addition food grade saleratus 10g, skimmed milk power 6g, oral glucose 20g are mixed；
B1. live vaccines of hog cholera head is carried out to piglet group to exempt from, after 1 bottle of live vaccines of hog cholera of 10ml diluted, take out 4ml (i.e.
Every pig 1200RID) it is added to the container, it mixes；
C1. mixture in container is poured into swinery drinks tank, freely drinks for piglet group to be immunized, is drunk in 1.5 hours
It finishes, observed after immunization piglet group 1h, no allergic reaction occurs.
Two exempt from environment temperature be 32 DEG C, air humidity 44% is fine, and amount of drinking water is about 30000ml/ days.Swine fever is living
The immunization method of vaccine, includes the following steps：
A2. piglet group to be immunized is cut off the water supply 2 hours, measures 12000ml purified water and be put into clean plastic containers, then in container
Middle addition food grade saleratus 40g, skimmed milk power 16g, oral glucose 80g are mixed；
B2. live vaccines of hog cholera two is carried out to piglet group to exempt from, after 1 bottle of live vaccines of hog cholera of 10ml diluted, take out 4ml (i.e.
Every pig 750RID) it is added to the container, it mixes；
C2. mixture in container is poured into swinery drinks tank, freely drinks for piglet group to be immunized, has been drunk in 1 hour
Finish, observed after immunization piglet group 1h, no allergic reaction occurs.
The detection of 4 hog cholera antibody of embodiment
In second it is immune after 7d, 14d, 28d, 42d and 56d, using Ai Deshi antibody against swine fever virus detection kit detection the
One group, second group, third group and the hog cholera antibody for injecting control group.
1 hog cholera antibody blocking rate result of table
Remarks：Blocking rate >=40% is the positive；Blocking rate < 30% is feminine gender；Blocking rate is suspicious between 30-40%.
Testing result shows that drinking-water is immune to generate immune response with injecting immune, 14 days generation swine fevers after immune
Protection antibody, antibody level reached maximum protection at 42 days and continue to maintain, it is seen that two kinds of immunization method antibody generate horizontal
Without significant difference；But present invention drinking-water is immune not to be had to drive pig, grabs pig, stimulates small, raising Immune efficiency and epidemic prevention worker's work
Intensity, method is simple to operation, is suitble to group's epidemic prevention application.
The embodiment of the above, only presently preferred embodiments of the present invention, is only used to explain the present invention, not limit
The scope of the present invention processed to those of ordinary skill in the art certainly can be according to skill disclosed in this specification
Art content makes other embodiments easily by way of replacing or changing, therefore all made in the principle of the present invention
Changes and improvements etc., should be included in scope of the present invention patent.
Claims (3)
1. a kind of immunization method of live vaccines of hog cholera, which is characterized in that include the following steps：
A. piglet group to be immunized is cut off the water supply 2 ~ 4 hours, the purified water for measuring piglet group's daily drink amount 20% ~ 40% to be immunized is put
Enter in container, then according to the volume of purified water, adds 1 ~ 4g/L of food grade saleratus, 1 ~ 2g/L of skimmed milk power, mouth in a reservoir
4 ~ 7g/L of glucose is taken, is mixed；
B. piglet group's progress live vaccines of hog cholera head is exempted from or two exempts from, appearance will be added after the live vaccines of hog cholera dilution of suitable RID dosage
In device, mix；
C. mixture in container is poured into piglet group's drinks tank, freely drinks for piglet group to be immunized, is drunk in 1 ~ 2 hour
It finishes, observed after immunization piglet group 1h is disposed in time to occurring anaphylactoid piglet individually.
2. the immunization method of live vaccines of hog cholera according to claim 1, which is characterized in that step b is specially：To piglet group
Progress live vaccines of hog cholera head exempts from or two exempt from, and will be added to the container after the live vaccines of hog cholera dilution of every pig 750-1500RID dosage,
It mixes.
3. the immunization method of live vaccines of hog cholera according to claim 1, which is characterized in that in the piglet group to be immunized
Piglet is health pig.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201811146800.9A CN108904794A (en) | 2018-09-29 | 2018-09-29 | The immunization method of live vaccines of hog cholera |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811146800.9A CN108904794A (en) | 2018-09-29 | 2018-09-29 | The immunization method of live vaccines of hog cholera |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114868703A (en) * | 2022-05-05 | 2022-08-09 | 杭州市原种场 | Method for preventing and controlling swine fever infection of live pigs |
Citations (3)
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|---|---|---|---|---|
| CN102000332A (en) * | 2010-11-16 | 2011-04-06 | 南京农业大学 | Composite adjuvant for duck bird flu oral mucosal immunization |
| WO2014003536A1 (en) * | 2012-06-26 | 2014-01-03 | Biovalence Sdn. Bhd. | Dosage regime of fusion compounds |
| CN106310250A (en) * | 2015-06-19 | 2017-01-11 | 上海市农业科学院 | Swine fever oral attenuated freezing-dry vaccine and preparation method thereof and freeze-drying protective agent |
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2018
- 2018-09-29 CN CN201811146800.9A patent/CN108904794A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102000332A (en) * | 2010-11-16 | 2011-04-06 | 南京农业大学 | Composite adjuvant for duck bird flu oral mucosal immunization |
| WO2014003536A1 (en) * | 2012-06-26 | 2014-01-03 | Biovalence Sdn. Bhd. | Dosage regime of fusion compounds |
| CN106310250A (en) * | 2015-06-19 | 2017-01-11 | 上海市农业科学院 | Swine fever oral attenuated freezing-dry vaccine and preparation method thereof and freeze-drying protective agent |
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| 元进英: "《制药工艺学》", 30 June 2007, 化学工业出版社 * |
| 王永芬等: "《动物生物制品技术》", 31 August 2011, 中国农业大学出版社 * |
| 邢兰君: "秋冬季节猪易流行的传染病及防治-上", 《河北畜牧兽医》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114868703A (en) * | 2022-05-05 | 2022-08-09 | 杭州市原种场 | Method for preventing and controlling swine fever infection of live pigs |
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