CN108904552A - The preparation method of triterpenes components in a kind of fuling peel - Google Patents
The preparation method of triterpenes components in a kind of fuling peel Download PDFInfo
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- CN108904552A CN108904552A CN201810773863.0A CN201810773863A CN108904552A CN 108904552 A CN108904552 A CN 108904552A CN 201810773863 A CN201810773863 A CN 201810773863A CN 108904552 A CN108904552 A CN 108904552A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/10—Antioedematous agents; Diuretics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a kind of preparation methods of triterpenes components in fuling peel, and steps are as follows：The Poria Peel methanol cold soaking of 60-70% is extracted 1-3 times, each 1-3d, combined extract is concentrated under reduced pressure to give medicinal extract；It is extracted with ethyl acetate 2-4 times after medicinal extract is dissolved with suitable water, combining extraction liquid, is concentrated under reduced pressure, obtains acetic acid ethyl ester extract；It is successively 65 with volume ratio by ethyl acetate portion through silica gel chromatograph post separation:1,45:1,25:1,15:1,5:1 chloroform-methanol elution, collects 15:1 elution fraction, is concentrated to obtain the final product.The preparation method can be enriched with triterpenes components in fuling peel, have diuresis, oxidation resistant effect, can be used for drug preparation.In addition, this method is easy to operate, can implement on a large scale.
Description
Technical field
The present invention relates to a kind of preparation methods of triterpenes components in fuling peel.
Background technique
Poria cocosPoria cocos (Schw.) Wolf is also known as Fu Tu, Fu spirit, Yun Ling, Song Ling etc., belongs on taxology
Aphyllophorales Aphyllophorales, Polyporaceae Polyporaceae, Poria cocos belong to Wolfiporia.Be mainly distributed on Yunnan,
Hubei, Anhui, Shandong, Hebei, Henan etc. save.Its dry sclerotia is known as " PORIA ALBA ", and the crust of sclerotium is fuling peel.To go through version
《Chinese Pharmacopoeia》It is recorded.Poria cocos is the traditional Chinese medicine in China, mild-natured, sweet in flavor, light, return heart, lung, spleen, kidney channel；With Li Shui
The effect of excreting dampness, strengthening spleen and middle warmer, calming heart and tranquilizing mind is the raw material of a variety of prescriptions and Chinese patent drug, has " ten medicines, nine Poria cocos " one to say.With Fu
Siberian cocklebur is compared, and fuling peel is more good at inducing diuresis for removing edema.Poria cocos is used as medicine, first recorded in《Sheng Nong's herbal classic》, it is classified as top grade, claims its " long term usage peace
Soul is reposed, not hungry macrobiosis ".Record about fuling peel mainly has：《Traditional Chinese Medicine in Sichuan will》Record fuling peel " mild-natured, slightly sweet flavor, nothing
Poison.Enter kidney, bladder two passes through ".《Detailed outline》It records fuling peel " main oedema general edema, channell, loosing the interspaces of skin and muscles ".《Medical center bun is wanted》Record Fu
Siberian cocklebur skin " water of row skin ".
The chemical component of Poria cocos mainly has pachymose, fuling triterpene（Pachymic acid, native not acid etc.）, natural gum, protein and rouge
Fat acid etc..And fuling peel master acids containing triterpene and carbohydrate content, wherein Triterpene acids wide variety.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation methods of triterpenes components in fuling peel.
The present invention is realized by following technical solution：
The preparation method of triterpenes components, includes the following steps in a kind of fuling peel：By the methanol of Poria Peel 60-70%
Cold soaking extracts 1-3 times, and each 1-3d, combined extract is concentrated under reduced pressure to give medicinal extract；Second is used after medicinal extract is dissolved with suitable water
Acetoacetic ester extracts 2-4 times, combining extraction liquid, is concentrated under reduced pressure, obtains acetic acid ethyl ester extract；By ethyl acetate portion through silica gel color
Post separation is composed, is successively 65 with volume ratio:1,45:1,25:1,15:1,5:1 chloroform-methanol elution, collects 15:1 elution portion
Point, it is concentrated to obtain the final product.
Preferably, in the preparation method, 65% methanol cold soaking of Poria Peel is extracted.
Preferably, in the preparation method, methanol cold soaking is extracted 2 times.
Preferably, in the preparation method, 2d is extracted every time.
Preferably, in the preparation method, 1kg fuling peel is extracted using 5-9L solvent.
Preferably, in the preparation method, 1g medicinal extract 8-10mL water dissolves.
The technology of the present invention effect：
The preparation method can be enriched with triterpenes components in fuling peel, have diuresis, oxidation resistant effect, can be used for drug system
It is standby.In addition, this method is easy to operate, can implement on a large scale.
Specific embodiment
Essentiality content of the invention is specifically introduced below with reference to embodiment.
Embodiment 1
The preparation method of triterpenes components, includes the following steps in a kind of fuling peel：By Poria Peel with 65% methanol cold soaking
It extracts 2 times, each 2d, combined extract is concentrated under reduced pressure to give medicinal extract；Extracted after medicinal extract is dissolved with suitable water with ethyl acetate
It takes 3 times, combining extraction liquid, is concentrated under reduced pressure, obtains acetic acid ethyl ester extract；By ethyl acetate portion through silica gel chromatograph post separation,
It is successively 65 with volume ratio:1,45:1,25:1,15:1,5:1 chloroform-methanol elution, collects 15:1 elution fraction, concentration are
?.
Wherein, 1kg fuling peel is extracted using 7L solvent, and 1g medicinal extract 9mL water dissolves.
Embodiment 2
The preparation method of triterpenes components, includes the following steps in a kind of fuling peel：By Poria Peel with 60% methanol cold soaking
It extracts 1 time, each 1d, combined extract is concentrated under reduced pressure to give medicinal extract；Extracted after medicinal extract is dissolved with suitable water with ethyl acetate
It takes 2 times, combining extraction liquid, is concentrated under reduced pressure, obtains acetic acid ethyl ester extract；By ethyl acetate portion through silica gel chromatograph post separation,
It is successively 65 with volume ratio:1,45:1,25:1,15:1,5:1 chloroform-methanol elution, collects 15:1 elution fraction, concentration are
?.
Wherein, 1kg fuling peel is extracted using 5L solvent, and 1g medicinal extract 8mL water dissolves.
Embodiment 3
The preparation method of triterpenes components, includes the following steps in a kind of fuling peel：By Poria Peel with 70% methanol cold soaking
It extracts 3 times, each 3d, combined extract is concentrated under reduced pressure to give medicinal extract；Extracted after medicinal extract is dissolved with suitable water with ethyl acetate
It takes 4 times, combining extraction liquid, is concentrated under reduced pressure, obtains acetic acid ethyl ester extract；By ethyl acetate portion through silica gel chromatograph post separation,
It is successively 65 with volume ratio:1,45:1,25:1,15:1,5:1 chloroform-methanol elution, collects 15:1 elution fraction, concentration are
?.
Wherein, 1kg fuling peel is extracted using 9L solvent, and 1g medicinal extract 10mL water dissolves.
The preparation method can be enriched with triterpenes components in fuling peel, have diuresis, oxidation resistant effect, can be used for medicine
Product preparation.In addition, this method is easy to operate, can implement on a large scale.
Claims (6)
1. the preparation method of triterpenes components in a kind of fuling peel, it is characterised in that include the following steps：Poria Peel is used
The methanol cold soaking of 60-70% extracts 1-3 times, and each 1-3d, combined extract is concentrated under reduced pressure to give medicinal extract；By medicinal extract with suitable
It is extracted with ethyl acetate 2-4 times after water dissolution, combining extraction liquid is concentrated under reduced pressure, obtains acetic acid ethyl ester extract；By ethyl acetate
Part is successively 65 with volume ratio through silica gel chromatograph post separation:1,45:1,25:1,15:1,5:1 chloroform-methanol elution, is received
Collection 15:1 elution fraction, is concentrated to obtain the final product.
2. preparation method according to claim 1, it is characterised in that：65% methanol cold soaking of Poria Peel is extracted.
3. preparation method according to claim 2, it is characterised in that：Methanol cold soaking extracts 2 times.
4. preparation method according to claim 3, it is characterised in that：2d is extracted every time.
5. preparation method according to claim 1, it is characterised in that：1kg fuling peel is extracted using 5-9L solvent.
6. preparation method according to claim 1, it is characterised in that：1g medicinal extract 8-10mL water dissolves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810773863.0A CN108904552A (en) | 2018-07-15 | 2018-07-15 | The preparation method of triterpenes components in a kind of fuling peel |
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| CN201810773863.0A CN108904552A (en) | 2018-07-15 | 2018-07-15 | The preparation method of triterpenes components in a kind of fuling peel |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111494393A (en) * | 2019-01-30 | 2020-08-07 | 中国科学院微生物研究所 | Preparation method and application of poria peel triterpene composition |
-
2018
- 2018-07-15 CN CN201810773863.0A patent/CN108904552A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111494393A (en) * | 2019-01-30 | 2020-08-07 | 中国科学院微生物研究所 | Preparation method and application of poria peel triterpene composition |
| CN111494393B (en) * | 2019-01-30 | 2021-05-07 | 中国科学院微生物研究所 | Preparation method and application of poria peel triterpene composition |
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