CN108902473A - A kind of application of sheep forage and Herba Origani Vulgaris quintessence oil - Google Patents

A kind of application of sheep forage and Herba Origani Vulgaris quintessence oil Download PDF

Info

Publication number
CN108902473A
CN108902473A CN201810590633.0A CN201810590633A CN108902473A CN 108902473 A CN108902473 A CN 108902473A CN 201810590633 A CN201810590633 A CN 201810590633A CN 108902473 A CN108902473 A CN 108902473A
Authority
CN
China
Prior art keywords
parts
sheep
quintessence oil
herba origani
vulgaris quintessence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810590633.0A
Other languages
Chinese (zh)
Inventor
焦婷
雷赵民
吴建平
梁建勇
冉福
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gansu Agricultural University
Original Assignee
Gansu Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gansu Agricultural University filed Critical Gansu Agricultural University
Priority to CN201810590633.0A priority Critical patent/CN108902473A/en
Publication of CN108902473A publication Critical patent/CN108902473A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention discloses the applications of a kind of sheep forage and Herba Origani Vulgaris quintessence oil, are related to field of feed.A kind of sheep forage of the invention includes the raw material of following parts by weight：60-90 parts of ensiling, 10-20 parts of corn, 2-6 parts of wheat bran, 2-6 parts of rapeseed meal, 2-6 parts of cotton dregs, 0.1-1 parts of 1% premix, 0.1-1 parts of calcium monohydrogen phosphate, 0.1-1 parts of mountain flour, 0.1-1 parts of salt, 0.1-0.5 parts of Herba Origani Vulgaris quintessence oil.The above-mentioned feed formula of design of the invention, by adjusting being formulated and add Herba Origani Vulgaris quintessence oil, obtained sheep forage is easy to digest, and silage corn blade epidermis is degradable by rumen microorganism, the part that mesophyll disappears increased significantly, and improve sheep digestion power, absorbability, sheep digestive disease is reduced, sheep health is promoted.

Description

A kind of application of sheep forage and Herba Origani Vulgaris quintessence oil
Technical field
The present invention relates to the applications of field of feed more particularly to a kind of sheep forage and Herba Origani Vulgaris quintessence oil.
Background technique
Herba Origani Vulgaris quintessence oil Essential Oil (EO) is a kind of volatile plant essence extracted in the flower and leaf of wild marjoram grass Oil, main ingredient are phenol type substances, and carvacrol accounts for 79.6%, and cymene accounts for 8.7%, and thymol accounts for 2.5%, terpene Product alkene 2.1%.In addition also containing terpenes, linalool, terpene alcohol, aliphatic ketone and cyclic ethers classes such as α-terpinene, laurene, cumic acids Compound etc..
Currently, sheep feed formulation is unreasonable, easily leads to sheep digestive system and generate various diseases, such as indigestion, tumor Gaseous distention, grainsick and sheep absorption difference etc., it is low that meat of a sheep amount lacks quality, influences income.
Summary of the invention
In view of this, main purpose is solution the embodiment of the invention provides the application of a kind of sheep forage and Herba Origani Vulgaris quintessence oil The certainly low and nondigestible problem of sheep forage nutrition.
In order to achieve the above objectives, invention broadly provides following technical solutions：
On the one hand, the embodiment of the invention provides a kind of sheep forages comprising the raw material of following parts by weight：
60-90 parts of ensiling, 10-20 parts of corn, 2-6 parts of wheat bran, 2-6 parts of rapeseed meal, 2-6 parts of cotton dregs, 1% premix 0.1- 1 part, 0.1-1 parts of calcium monohydrogen phosphate, 0.1-1 parts of mountain flour, 0.1-1 parts of salt, 0.1-0.5 parts of Herba Origani Vulgaris quintessence oil.
Preferably, the feed includes the raw material of following parts by weight：
65-80 parts of ensiling, 12-18 parts of corn, 2-4 parts of wheat bran, 2-4 parts of rapeseed meal, 2-5 parts of cotton dregs, 1% premix 0.1-0.5 parts, 0.1-0.5 parts of calcium monohydrogen phosphate, 0.1-0.5 parts of mountain flour, 0-0.5 parts of salt, 0.1-0.4 parts of Herba Origani Vulgaris quintessence oil.
Preferably, the feed includes the raw material of following parts by weight：
70-75 parts of ensiling, 13-15 parts of corn, 4 parts of wheat bran, 3-4 parts of rapeseed meal, 3-4 parts of cotton dregs, 1% premix 0.1- 0.3 part, 0.3-0.4 parts of calcium monohydrogen phosphate, 0.3-0.4 parts of mountain flour, 0.2-0.3 parts of salt, 0.24-0.43 parts of Herba Origani Vulgaris quintessence oil.
Preferably, the feed includes the raw material of following parts by weight：72-75 parts of ensiling, 13-15 parts of corn, wheat bran 4 Part, 3-4 parts of rapeseed meal, 3-4 parts of cotton dregs, 0.1-0.3 parts of 1% premix, 0.3-0.4 parts of calcium monohydrogen phosphate, mountain flour 0.3-0.4 Part, 0.2-0.3 parts of salt, 0.2 part of Herba Origani Vulgaris quintessence oil.
Preferably, the feed includes the raw material of following parts by weight：72-75 parts of ensiling, 13-15 parts of corn, wheat bran 4 Part, 3-4 parts of rapeseed meal, 3-4 parts of cotton dregs, 0.1-0.3 parts of 1% premix, 0.3-0.4 parts of calcium monohydrogen phosphate, mountain flour 0.3-0.4 Part, 0.2-0.3 parts of salt, 0.4 part of Herba Origani Vulgaris quintessence oil.
On the other hand, the application the embodiment of the invention provides a kind of Herba Origani Vulgaris quintessence oil in preparation sheep forage, the ox It is above-mentioned Herba Origani Vulgaris quintessence oil to essential oil.
Compared with prior art, the beneficial effects of the invention are as follows：
The above-mentioned feed formula of design of the invention, by adjusting being formulated and adding Herba Origani Vulgaris quintessence oil, obtained sheep forage It is easy to digest, and the silage corn leaf surface waxes layer in feed is destroyed, and the part that mesophyll disappears increased significantly, and mention High sheep digestion power, absorbability reduce sheep digestive disease, promote sheep health.
Detailed description of the invention
Fig. 1 is the histogram that the addition EO that the embodiment of the present invention 5 provides influences ensilage nutrient digestibility；
Fig. 2 is the histogram that the addition EO that the embodiment of the present invention 5 provides influences sheep rumen liquid pH；
Fig. 3 is the addition EO of the offer of the embodiment of the present invention 5 to sheep rumen liquid NH3The column that-N concentration (mg/100mL) influences Shape figure；
Fig. 4 is the column that the addition EO that the embodiment of the present invention 5 provides influences sheep rumen liquid VFA concentration (mmol/L) Figure；
Fig. 5 A- Fig. 5 F is that the different amounts of EO of addition that the embodiment of the present invention 5 provides sweeps the degradation of silage corn leaf tissue Retouch electron microscope；
Fig. 6 A- Fig. 6 F is that silage corn fringe hide fiber structure drops in the different amounts of EO of addition that the embodiment of the present invention 5 provides The scanning electron microscope (SEM) photograph of solution.
Specific embodiment
For further illustrate the present invention to reach the technical means and efficacy that predetermined goal of the invention is taken, below with compared with Good embodiment, to specific embodiment, technical solution, feature and its effect applied according to the present invention, detailed description is as follows. The special characteristic, structure or feature in multiple embodiments in following the description can be combined by any suitable form.
Embodiment 1 (feed formula)
A kind of sheep forage, the raw material including following parts by weight：
60 parts of ensiling, 10 parts of corn, 2 parts of wheat bran, 6 parts of rapeseed meal, 6 parts of cotton dregs, 0.1 part of 1% premix, calcium monohydrogen phosphate 0.1 part, 0.1 part of mountain flour, 0.1 part of salt, 1 part of Herba Origani Vulgaris quintessence oil.
Embodiment 2 (feed formula)
A kind of sheep forage, the raw material including following parts by weight：
90 parts of ensiling, 20 parts of corn, 6 parts of wheat bran, 6 parts of rapeseed meal, 6 parts of cotton dregs, 1 part of 1% premix, calcium monohydrogen phosphate 1 Part, 1 part of mountain flour, 1 part of salt, 10 parts of Herba Origani Vulgaris quintessence oil.
Embodiment 3 (feed formula)
A kind of sheep forage, the raw material including following parts by weight：
73 parts of ensiling, 14.4 parts of corn, 4 parts of wheat bran, 3.6 parts of rapeseed meal, 3.9 parts of cotton dregs, 0.29 part of 1% premix, phosphorus 0.31 part of sour hydrogen calcium, 0.31 part of mountain flour, 0.28 part of salt, 4 parts of Herba Origani Vulgaris quintessence oil.
Embodiment 4 (feed formula)
A kind of sheep forage, the raw material including following parts by weight：
73 parts of ensiling, 14.4 parts of corn, 4 parts of wheat bran, 3.6 parts of rapeseed meal, 3.9 parts of cotton dregs, 0.29 part of 1% premix, phosphorus 0.31 part of sour hydrogen calcium, 0.31 part of mountain flour, 0.28 part of salt, 7 parts of Herba Origani Vulgaris quintessence oil.
Embodiment 5 (feed quality of Herba Origani Vulgaris quintessence oil embodiment 1-3 and the influence to Ruminal Fermentation of Sheep characteristic)
Experimental animal is progress rumen fistula operation in 3 months in advance, installs 3 healthy ram (Germany's beauty of rumen fistula Sharp slave ♂ × Lintao sheep ♀ 2 generation of hybridization), average weight 53.68kg, it is 39.8 DEG C that rectum, which measures mean body temperature,；
The Herba Origani Vulgaris quintessence oil additive that the present invention selects is purchased from Ralco company of the U.S.；Its main component and composition：Containing 1.13% Herba Origani Vulgaris quintessence oil (diameter<It is 5 microns, uniform in size, have a small amount of olive oil it is micro- it is molten wherein)+1.87% herbal medicine packet of+97% carrier).
Use Herba Origani Vulgaris quintessence oil additive (EO) in test, if 3 processing, 3 levels are added in each processing, i.e. 0g, 4g with 7g respectively throws 3 repetitions of every processing in 3 examination sheep cuds, totally 3 Fistulated sheeps respectively, and number is 1,2,3 (such as 1 institute of table respectively Show)；The error that difference and different times silage corn attenuation degree difference may cause is digested to eliminate experimental animal individual, Test uses Latin―Square design, and every 3 phases of examination sheep point add different additive amount in turn；Experimental design is shown in Table 1.
1 experimental design of table
Feeding management：Divide 3 groups for examination sheep, every group 3, is placed in colony house raising.Fistulated sheep feeds the (morning 9 twice daily:00 With afternoon 5:00), special messenger is responsible for, free water.
Fistulated sheep diet：Fistulated sheep diet is according to People's Republic of China's agricultural industry criteria mutton sheep feeding standard (NY/ T816-2004 ram hogg(et) nutritional requirements (weight 55kg, daily gain 50g) is formulated in), and experimental period is for trying Fistulated sheep For the essence that feeding is prepared slightly than being 3 ︰ 7 (concentrate supplement+silage corn, 1.6kg/ head/d), wherein ensilage quality is good, is in Yellow green has sour taste, soft.It is formed and trophic level is shown in Table 2.
Table 2 tests diet composition and trophic level (%, air bells dry basis)
The preparation of sample：Before every wheel formal test starts, the random several points acquisition whole corn silage sample in silage silo will Fresh sample is taken back stir 60~80s with high-speed mixer after laboratory prescinds immediately.After crushing, point 3 parts, a part is used In dress Nylon Bag, each packed 4g of nylon, 3 repetitions, i.e. 3 Nylon Bags in every examination sheep cud are numbered, such 3 respectively Examination sheep needs 36g altogether, while eliminating systematic error not fill the empty Nylon Bag of any sample as control；A part of ensiling sample freezing It saves, for measuring wherein VFA (acetic acid, propionic acid, butyric acid) and NH3-N；65 DEG C of Part III drying, measurement wherein DM, CP, ADF and NDF content.
Intact maize leaf and Sui Pideng corn tissue are several after selection silage fermentation, make each group selected Surface of second order product is sufficiently large, can cut the sample block of 3 1cm × 1cm sizes, i.e. 3 repetitions in same tissue.
Rumen Nylon Bag：Nylon Bag selects the nylon cloth of 38 μm of eyelet (400 mesh), is tailored into 6.5cm × 9.5cm size Nylon Bag and nylon rope is housed, dissipate side with burning hot.
Nylon Bag weighing：It will be placed in air dry oven (65 DEG C) 2h by the Nylon Bag of number, after wearing nylon gloves taking-up It is put into dry 30min, weighing in drying basin rapidly and records weight.It is placed in baking oven 30min again, takes out dry 30min, then claim Weight.The difference of weight is less than 0.002g twice.Otherwise it repeats the above steps, until constant weight.
It fills sample and puts bag：1cm × 1cm the corn tissue that accurately weighs 4g or so fresh sample to be measured with template and will shear (blade and fringe skin sample) is fitted into the Nylon Bag numbered in advance, is slowly packed into Nylon Bag, tie-down sack, and 7 one group of Nylon Bag It ties up on a preprepared key ring, 7 sacks is thrown in cud by cud fistula, key chain sheep body outside fistula To fix and facilitate taking-up Nylon Bag on rope.
The dispensing of Herba Origani Vulgaris quintessence oil：Different additive 4g and 7g packet is accurately weighed in paper, is pressed while launching Nylon Bag It is put into different sheep rumens respectively according to experimental design by rumen fistula, then breaks paper release additives with tweezers.
The time of placement：(native grass is degraded in cud after 48h, and it is dry by residence time 48h of the Nylon Bag in cud Substance rumen digestibility tends towards stability), Nylon Bag is taken out after 48h to be rinsed.
Sample acquisition：The Nylon Bag for indicating fresh sample taking-up is reinstated 39 DEG C of warm water threads together with nylon rope one gently to rinse, Until clarification of water.It is put into 65 DEG C of air dry oven and dries after cleaning, until constant weight, the Nylon Bag after constant weight is received Then collection collects the residue of sack in every examination sheep cud, 3 of each sample duplicate 3 bags of residues is mixed, are crushed, mistake 0.45mm sieve, the content for DM, CP, ADF and NDF in residue to be measured.
It is put immediately into cold water after being taken out equipped with the Nylon Bag of maize leaf and fringe, terminates fermentation.It is thin with 39 DEG C of warm water Stream gently rinses, until Nylon Bag surface is clean, it is beautiful to collect the 1cm × 1cm being equipped in advance in Nylon Bag after lumen fermentation 48h Rice tissue-like sheet, finds out according to the number of Nylon Bag and is derived from 3 different additive levels (0g, 4g and 7g) of same maize leaf The sample prescription of processing, clear water take back laboratory after cleaning, the variation of its fibrocyte wall construction are observed under scanning electron microscope.
Rumen fluid acquisition：While taking out other samples, rumen fluid 50mL is extracted from examination sheep cud by fistula, immediately Measure the pH value of rumen fluid.Then with 4 layers of filtered through gauze, the rumen fluid collection of impurity is filtered off in sampling bottle, is put into refrigerator (- 20 DEG C) in freezen protective, for measuring the NH in rumen fluid3- N and VFA.
Sample index determining：
Dry matter uses oven drying method；Crude protein is measured using Micro-kjoldahl method；Acid detergent fiber and neutrality are washed Fiber is washed to measure using Van soest method.Measure respectively DM, CP, NDF and ADF ruminal digestion rate DMD, CPD, NDFD and ADFD。
The measurement of lumen fermentation characteristic：
The measurement of rumen fluid pH value：It is measured using HI98103 data type pen type acidometer.
The measurement of NH3-N concentration in rumen fluid：It is measured according to the kind improved colorimetric method of Feng ancestor.The mark that this test measures Directrix curve is y=0.3815x-0.0084 (R2=0.9988).In formula：Y is extinction value, and x is ammonia nitrogen concentration (mg/100mL). This test rumen fluid sampling amount is 2mL, 5 times is diluted, with 721 type spectrophotometric colos, wavelength 700nm.
The measurement of VFA concentration in rumen fluid：Cud is measured using Agilent (Agilent1100), high performance liquid chromatograph The content of VFA in liquid.Test method and chromatographic condition refer to Bai Xue.
The maize leaf fibre structure observation of fermentation front and back：Using stereomicroscope (Discovery.V12) and scanning electron microscope (S-3400N) observation silage corn tissue fibers degradation situation.
Data statistic analysis：After all data are pre-processed with Excel 2013, carried out using 19.0 software package of SPSS The one-factor analysis of variance when significant difference, carries out Multiple range test using Duncan method, with P<0.05 sentences as the significance of difference Disconnected standard；It is mapped with SigmaPlot 12.5.
As a result with analysis：
It will be seen from figure 1 that NDFD and ADFD, after adding Herba Origani Vulgaris quintessence oil, 4g group and 7g group silage corn are compared with 0g group phase Than significantly improving (P<0.05), and occur first increasing the trend reduced afterwards, 10.41% and has been respectively increased in 4g group NDFD, ADFD 9.60% and 9.76% has been respectively increased in 13.69%, 7g group, it can be seen that 4g group increases the most obvious；And 4g group and 7g group it Between be not significantly different (P>0.05).Not significant (the P of difference between DMD and CPD is respectively handled>0.05), but totally also occur after first rising The trend of drop.
Figure it is seen that examination Liquid in sheep rumen pH value 7g group is significantly higher than 0g group and 4g group (P after addition Herba Origani Vulgaris quintessence oil< 0.05) not significant (P of difference, and between 0g group and 4g group>0.05).
As seen from Figure 3, with the increase of Herba Origani Vulgaris quintessence oil additive amount, NH3Decreasing trend, 7g group is totally presented in-N concentration (P is significantly reduced than 0g group and 4g group<0.05).
Fig. 4 is shown, adds 4g and 7g Herba Origani Vulgaris quintessence oil sheep rumen liquid TVFA and propionic acid is significantly higher than control group, acetic acid/the third It is sour then show opposite variation (P<0.05), acetic acid and the not significant (P of butyric acid content difference between different disposal>0.05).
Add influence of the EO to silage corn leaf fiber structural degradation：As shown in figure 5, can be seen that from Fig. 5 A to 5B The silage corn leaf surface waxes layer for being not added with EO is degraded by rumen microorganism to be destroyed, no epidermal hair.In the stereoscopic Electronic Speculum of Fig. 5 A Under be apparent that the thicker presentation yellow in mesophyll part, reticular structure is covered by mesophyll.In conjunction with Fig. 5 B it is found that being not added with In the case where EO, silage corn blade surface layer is more rough, is in nature, is partially digested and sponges, but mesophyll, vein Structural integrity.
By Fig. 5 C to 5D it is found that silage corn leaf surface waxes layer is destroyed, no epidermal hair after addition 4g EO, own Epidermis is decomposed by rumen microorganism substantially, and the part that mesophyll disappears increased significantly, and mesophyll part becomes thin and transparent.? Under the stereoscopic Electronic Speculum of Fig. 5 C, it can be clearly seen that entire blade is thin and transparent, and reticular structure is high-visible.In Fig. 5 D scanning electron microscope Under, occur palisade tissue completely and be partially destroyed spongy tissue occur, cell arrangement is in disorder, and the histocyte wall being destroyed is clear It is clear as it can be seen that vein structural integrity.
By Fig. 5 E to 5F it is found that epidermal cell, palisade tissue, spongy tissue are destroyed and molten completely after addition 7g EO Solution, only remains netted vein frame, and vein structure becomes thin and transparent.
Add influence of the EO to silage corn fringe hide fiber structural degradation:By Fig. 6 A to 6B it is found that being not added with the ensiling of EO Corncob epidermis face wax coat is degraded by rumen microorganism and is destroyed, no epidermal hair, and part epidermal cell destroys, the table not destroyed Cells line is neat, is in nature extended state, mesophyll, vein structural integrity.From Fig. 6 A as it can be seen that corncob epidermis face is smooth And it is transparent, part mesophyll disappears between some veins.From Fig. 6 B it is found that corncob skin mesophyll part has disappeared, it is left surface Coarse vein structure arranges in disorder.
By Fig. 6 C to 6D it is found that the silage corn fringe epidermis chrotoplast of addition 4g EO is destroyed, palisade tissue occurs and portion Divide and be destroyed, palisade tissue cell wall is damaged, and vein profile is complete.From Fig. 6 C as it can be seen that the part that mesophyll disappears on fringe skin becomes larger And quantity increases.From Fig. 6 D it is found that mesophyll part has disappeared between vein, vein rough surface is uneven.
By Fig. 6 E to 6F it is found that silage corn fringe epidermis chrotoplast, palisade tissue, the spongy tissue of addition 7g EO are complete It is destroyed and dissolves, only surplus parallel shape vein frame, and arrange in disorder.
Add influence of the Herba Origani Vulgaris quintessence oil EO to DMD, CPD, NDFD and ADFD
After the EO for adding different level in present invention test, sheep does not have DMD, CPD, NDFD, ADFD of silage corn Make a significant impact (P>0.05), but after addition 4g DMD and CPD increases than control group, has been respectively increased 0.46%, 2.98%.These are all related with the mechanism of action of EO, and EO can stimulate animal appetite, effectively activation digestive ferment, make the sticky of chyme Degree changes, and nutriment in feed is promoted to fully absorb.Enzymatic activity increases in animal alimentary canal, improves the benefit of feed With rate, to improve the utilization rate of nutrient in feed.In addition, EO has special ethereal oil fragrance, it can also play and lure The effect of food, special fragrance pass to the nerve center of animal brain by olfactory organ, cause point of glandula digestive in alimentary canal Secrete digestive juice.The present invention is to study sheep, it is unobvious to the degradation difference of feed nutrient by addition different level EO, This may be related with the mechanism of action of animal species, the pitch-based sphere of EO and different animals.
Add influence of the EO to sheep rumen liquid pH concentration：Rumen fluid ph is to reflect ruminant tumor gastric fermentation level most Generally regular variation can be presented with feed type in time after feeding and diet in intuitive index, and variation range substantially exists Between 5.0 to 7.5.In present invention experiment, EO is added in 48h, (P is not made a significant impact on sheep rumen liquid pH value> 0.05) after, adding EO, the sheep rumen liquid pH of 4g is substantially less than 7g (P<0.05).The change of each processing group examination Liquid in sheep rumen pH value Change and also declines with horizontal increase of additive, and after adding EO, pH value is first reduced and is increased afterwards.This may be with the feeding of sheep Measure related, although diet or will affect sheep rumen liquid pH using additive, influence of the feed intake to rumen fluid ph is more bright It is aobvious.
It is increased afterwards in addition, EO first reduces the variation of sheep rumen liquid pH value with the horizontal increase of additive；In addition in body For same additive capacity in outer culture, with the extension of incubation time, medium pH value is gradually decreased, and all normal Rumen Internal Environment level in.
EO is added to sheep rumen liquid NH3The influence of-N concentration：Protein is one of most important nutrient of animal, dynamic There is very important biological function in object.Crude protein in daily ration is the main source of ammonia in cud, nitrogenous in diet After substance enters ruminant tumor gastric, can under the action of rumen microorganism, be degraded into oligopeptides, free amino acid and Ammonia etc., while rumen microorganism can also make full use of free amino acid and ammonia of degradation etc. to close under energy supply sufficiency At mycoprotein.Therefore, pass through total nitrogen, NH in measurement ruminant tumor gastric juice3- N, urea nitrogen and albumen nitrogen concentration are to evaluation Ruminant tumor gastric fermentation level, nitrogen substance degradation rate synthesize intensity etc. with microprotein and have very important significance. In present invention experiment, addition EO is to examination Liquid in sheep rumen NH3- N concentration has reduction examination sheep tumor all without generating significant impact Gastric juice NH3The trend of-N concentration mean value, and 2 addition processing groups try Liquid in sheep rumen NH3- N concentration does not add processing substantially below Group examination sheep.This may be the effects of having growth promotion, proliferation to rumen microorganism due to additive and increase activity, to make tumor The NH that stomach microorganism can more preferably be generated using ruminal degradation3Synthetic animalcule albumen matter, so there is addition group examination sheep cud Liquid NH3The lower phenomenon of-N concentration；In addition thymol acts on rumen microorganism and then influences the deamination work of amino acid With.
Add influence of the EO to sheep rumen liquid VFA：The concentration and many feed additions of VFA are generated in ruminant tumor gastric Agent close relation, the present invention experiment in add various dose EO to sheep rumen liquid TVFA, acetic acid, propionic acid, butyric acid and Second/the third does not make a significant impact (P>0.05), but add EO after be improved TVFA, acetic acid, propionic acid, butyric acid it is dense Degree and reduction second/third ratio trend (P>0.05).This illustrates that the other compositions of origanum oil may also play lumen fermentation Regulating and controlling effect.VFA be feed in cud by the important final product of microbial fermentation, in addition to being influenced by daily ration characteristic, also by tumor The influence of stomach microorganism growth.Therefore, promote the growth of rumen microorganism, feed lumen fermentation speed can be made to accelerate, to make VFA content increases.
The influence that addition EO degrades to silage corn fibre structure：Feed addition containing main functional component Herba Origani Vulgaris quintessence oil Plant complex phenolic can maintain the balance of microorganism system in cud in agent EO, and the degradation of plant cell wall is in microorganism It is completed jointly in group.It is fine by cell wall of the stereoscopic and scanning electron microscope to silage corn blade and fringe skin in present invention experiment The direct observation for tieing up structure digestible degree, being not added with the silage corn blade of EO, only surface wax coat is degraded by rumen microorganism It destroying, partial mesh topology is covered by mesophyll, and blade surface layer is more rough, and it is in nature, is partially digested and sponges, leaf Meat, vein structural integrity.After adding 4g EO, silage corn leaf surface waxes layer is destroyed, and all epidermises are by the micro- life of cud Object decomposes, and the part that mesophyll disappears increased significantly, and mesophyll part becomes thin and transparent, and after adding 7g EO, epidermis Cell, palisade tissue, spongy tissue are destroyed and dissolve completely, only remain netted vein frame, and vein structure become it is thin and It is transparent.Illustrate the degradation for plant cell wall, it may be possible to since micropopulation has played effect.
The present invention is obtained by the research of embodiment 5 to draw a conclusion：
1) (P is not made a significant impact on DMD, CPD, NDFD, ADFD after adding EO>0.05), but DMD and CPD is with adding The increase appearance of dosage first increases the trend (P reduced afterwards>0.05)；But it is on the rise with the increase of additive amount；Pass through addition Additive discovery, addition EO can be enhanced the abilities of digestive and absorption to nutriment, improve Stock genetics and breeding and feed conversion Rate, but it is unobvious to coarse-fibred digestion and absorption effect.
2) pH value for adding 7g EO sheep rumen liquid, which is significantly higher than, to be not added with.The pH value of sheep rumen liquid is all in variation model Within enclosing, guarantee normal Rumen Internal Environment.
EO test group is added after 48h to examination Liquid in sheep rumen NH3- N concentration does not generate significant impact (P>0.05), but Have the tendency that reducing mean value.
The EO for adding various dose is significant without generating to sheep rumen liquid TVFA, acetic acid, propionic acid, butyric acid and second/the third Influence (P>0.05).
3) addition Herba Origani Vulgaris quintessence oil EO has very good effect to the degradation of silage corn tissue fibers structure.It is not added with to ensiling jade The fibre structure digestible degree of rice is lighter, and addition 4g and 7g is partial digested to the fibre structure of silage corn, the portion that mesophyll disappears Point increase and area becomes larger；Even only remaining vein frame, and vein structure becomes thin and transparent.
Place, those skilled in the art can not select from the prior art to the greatest extent in the embodiment of the present invention.
Disclosed above is only a specific embodiment of the invention, but scope of protection of the present invention is not limited thereto, is appointed What those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, answer It is included within the scope of the present invention.Therefore, protection scope of the present invention should be with above-mentioned scope of protection of the claims It is quasi-.

Claims (6)

1. a kind of sheep forage, which is characterized in that the feed includes the raw material of following parts by weight：
60-90 parts of ensiling, 10-20 parts of corn, 2-6 parts of wheat bran, 2-6 parts of rapeseed meal, 2-6 parts of cotton dregs, 0.1-1 parts of 1% premix, 0.1-1 parts of calcium monohydrogen phosphate, 0.1-1 parts of mountain flour, 0.1-1 parts of salt, 0.1-0.5 parts of Herba Origani Vulgaris quintessence oil.
2. a kind of sheep forage as described in claim 1, which is characterized in that the feed includes the raw material of following parts by weight：
65-80 parts of ensiling, 12-18 parts of corn, 2-4 parts of wheat bran, 2-4 parts of rapeseed meal, 2-5 parts of cotton dregs, 1% premix 0.1-0.5 Part, 0.1-0.5 parts of calcium monohydrogen phosphate, 0.1-0.5 parts of mountain flour, 0-0.5 parts of salt, 0.1-0.4 parts of Herba Origani Vulgaris quintessence oil.
3. a kind of sheep forage as described in claim 1, which is characterized in that the feed includes the raw material of following parts by weight：
70-75 parts of ensiling, 13-15 parts of corn, 4 parts of wheat bran, 3-4 parts of rapeseed meal, 3-4 parts of cotton dregs, 0.1-0.3 parts of 1% premix, 0.3-0.4 parts of calcium monohydrogen phosphate, 0.3-0.4 parts of mountain flour, 0.2-0.3 parts of salt, 0.24-0.43 parts of Herba Origani Vulgaris quintessence oil.
4. a kind of sheep forage as described in claim 1, which is characterized in that the feed includes the raw material of following parts by weight： 72-75 parts of ensiling, 13-15 parts of corn, 4 parts of wheat bran, 3-4 parts of rapeseed meal, 3-4 parts of cotton dregs, 0.1-0.3 parts of 1% premix, phosphoric acid 0.3-0.4 parts of hydrogen calcium, 0.3-0.4 parts of mountain flour, 0.2-0.3 parts of salt, 0.2 part of Herba Origani Vulgaris quintessence oil.
5. a kind of sheep forage as described in claim 1, which is characterized in that the feed includes the raw material of following parts by weight： 72-75 parts of ensiling, 13-15 parts of corn, 4 parts of wheat bran, 3-4 parts of rapeseed meal, 3-4 parts of cotton dregs, 0.1-0.3 parts of 1% premix, phosphoric acid 0.3-0.4 parts of hydrogen calcium, 0.3-0.4 parts of mountain flour, 0.2-0.3 parts of salt, 0.4 part of Herba Origani Vulgaris quintessence oil.
6. a kind of application of Herba Origani Vulgaris quintessence oil in preparation sheep forage, which is characterized in that the Herba Origani Vulgaris quintessence oil is claim 1-5 Described in any item Herba Origani Vulgaris quintessence oils.
CN201810590633.0A 2018-06-09 2018-06-09 A kind of application of sheep forage and Herba Origani Vulgaris quintessence oil Pending CN108902473A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810590633.0A CN108902473A (en) 2018-06-09 2018-06-09 A kind of application of sheep forage and Herba Origani Vulgaris quintessence oil

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810590633.0A CN108902473A (en) 2018-06-09 2018-06-09 A kind of application of sheep forage and Herba Origani Vulgaris quintessence oil

Publications (1)

Publication Number Publication Date
CN108902473A true CN108902473A (en) 2018-11-30

Family

ID=64419102

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810590633.0A Pending CN108902473A (en) 2018-06-09 2018-06-09 A kind of application of sheep forage and Herba Origani Vulgaris quintessence oil

Country Status (1)

Country Link
CN (1) CN108902473A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110692821A (en) * 2019-10-21 2020-01-17 安徽农业大学 Feed additive for improving rumen fermentation efficiency of cattle
CN110771742A (en) * 2019-10-21 2020-02-11 安徽农业大学 Feed additive for reducing methane emission of rumen of cattle and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106234786A (en) * 2016-10-10 2016-12-21 江苏省农业科学院 Lamb fattening TMR fermented feed and preparation method thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106234786A (en) * 2016-10-10 2016-12-21 江苏省农业科学院 Lamb fattening TMR fermented feed and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
C.J.WANG等: "Influences of flavomycin, ropadiar, and saponin on nutrient digestibility, rumen fermentation, and methane emission from sheep", 《ANIMAL FEED SCIENCE AND TECHNOLOGY》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110692821A (en) * 2019-10-21 2020-01-17 安徽农业大学 Feed additive for improving rumen fermentation efficiency of cattle
CN110771742A (en) * 2019-10-21 2020-02-11 安徽农业大学 Feed additive for reducing methane emission of rumen of cattle and preparation method thereof
CN110771742B (en) * 2019-10-21 2022-12-20 安徽农业大学 Feed additive for reducing methane emission of rumen of cattle and preparation method thereof
CN110692821B (en) * 2019-10-21 2022-12-20 安徽农业大学 Feed additive for improving rumen fermentation efficiency of cattle

Similar Documents

Publication Publication Date Title
Rocha Filho et al. Can spineless forage cactus be the queen of forage crops in dryland areas?
JP2022511565A (en) New composition
CN101455270A (en) Plant fermentation feedstuff
CN109601743A (en) A method of production high quality pork feed is prepared using feed mulberry
CN110074268A (en) A kind of low ratio High-Forage Diets and preparation method thereof promoting fattening lamb weight gain
Aregheore et al. Effects of Erythrina variegata, Gliricidia sepium and Leucaena leucocephala on dry matter intake and nutrient digestibility of maize stover, before and after spraying with molasses
CN108902473A (en) A kind of application of sheep forage and Herba Origani Vulgaris quintessence oil
Sarwar et al. Influence of nitrogen fertilization and stage of maturity of mottgrass (Pennisetum purpureum) on its composition, dry matter intake, ruminal characteristics and digestion kinetics in cannulated buffalo bulls
Dixon et al. Effects of oilseed meals and grain–urea supplements fed infrequently on digestion in sheep: 2. Cereal straw diets
Pathoummalangsy et al. Effects of supplementation with rumen fermentable carbohydrate and sources of'bypass' protein on feed intake, digestibility and N retention in growing goats fed a basal diet of foliage of Tithonia diversifolia
Beauchemin Effects of ingestive and ruminative mastication on digestion of forage by cattle
Zhao et al. Effects of rice straw particle size on chewing activity, feed intake, rumen fermentation and digestion in goats
Moreira et al. Palatability and digestibility of horse diets containing increasing levels of citrus pulp
Arowolo et al. The effect of forage theoretical cut lengths on chewing activity, rumen fermentation, dissolved gases, and methane emissions in goats
Castro et al. Effect of either once or twice daily concentrate supplementation of wheat straw on voluntary intake and digestion in sheep
CN106998753A (en) The cow producing milk efficiency of raising
Habib et al. Effect of protein supplements of varying degradability on growth rate, wool yield and wool quality in grazing lambs
KR20070112994A (en) A probiotic complex comprising organic metabolites and the preparing process thereof
Milis et al. Effects of main protein, non-forage fibre and forage source on digestibility, N balance and energy value of sheep rations
Ferreira et al. Effect of maize processing on diet selection in cows
Wang et al. Dietary supplementation of fat increased milk fat percentage without affecting ruminal characteristics in Holstein cows in a warm tropical environment
da Silva et al. Protein-energy supplementation for lambs: Feed intake, ingestive behavior, rumen parameters and nutrient digestibility
Zendrato et al. The utilization of cassava by-products into complete ration on performances and feed digestibility of weaning male crossbred Landrace pigs
CN109123120A (en) A method of sheep feed is prepared using Jasmine slag
Ogunbosoye et al. The utilization of some tropical browse plants by pregnant West African dwarf goats in southern Nigeria

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20181130

WD01 Invention patent application deemed withdrawn after publication