CN108896679A - A kind of efficient liquid phase detection method of l-tyrosine - Google Patents
A kind of efficient liquid phase detection method of l-tyrosine Download PDFInfo
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- CN108896679A CN108896679A CN201810791820.5A CN201810791820A CN108896679A CN 108896679 A CN108896679 A CN 108896679A CN 201810791820 A CN201810791820 A CN 201810791820A CN 108896679 A CN108896679 A CN 108896679A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/52—Physical parameters
- G01N30/54—Temperature
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8818—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving amino acids
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Abstract
The present invention relates to a kind of efficient liquid phase detection method of l-tyrosine, chromatographic condition is：Using octadecylsilane chemically bonded silica chromatographic column；Using the mixed solution of phosphate buffer and methanol as mobile phase, and the volume ratio of phosphate buffer and methanol is 6: 3.5~4.5；Detection wavelength is 220~230nm；Column temperature is 25~35 DEG C；Flow rate of mobile phase：0.7~1.0mL/min.The method of the present invention can quickly and accurately determine the content of l-tyrosine, can reduce error, it is ensured that its is quality controllable, and it is easy to operate, testing cost is low, detection time is short, detection efficiency is high.
Description
Technical field
The invention belongs to chromatographic technology field more particularly to a kind of efficient liquid phase detection methods of l-tyrosine.
Background technique
L-tyrosine (Tyrosine, Tyr) belongs to aromatic series epoxide acid, in vivo can by phenylalanine it is inverted from,
It is the precursor for synthesizing neurotransmitter catecholamine, is a kind of indispensable amino acid of human body.L-tyrosine and its metabolite
Content have notable difference in the Diseases body fluid such as Healthy People and genetic disease, liver and kidney disease and malignant tumour.L-
The metabolic disorder of tyrosine can lead to the generation of a variety of genetic diseases, and can make one to know from experience and growth failure, mentally disabled occur
Phenomena such as, therefore generally start to pay attention to the addition of l-tyrosine in food both at home and abroad.Therefore, the detection hand of l-tyrosine is explored
Section is of great significance for the quality control of the food containing l-tyrosine.
The method of detection l-tyrosine mainly has automatic amino acid analyzer detection method, mass spectrography, nuclear magnetic resonance wave at present
Spectrometry and capillary electrophoresis etc., although these technologies using than wide, use expensive equipment, complex pretreatment expense
When, reagent consumption is big and analysis time is longer, detects insensitive when for mixing sample, detect limit for height, be not able to satisfy L- junket
Detection demand when histidine content is low.
Summary of the invention
For detecting in the prior art, expensive equipment existing for l-tyrosine, complex pretreatment, reagent consumption are big, analyze
The problem of time is long, mixing sample detection is insensitive, detection limit for height, the present invention provide a kind of efficient liquid phase detection of l-tyrosine
Method.
To achieve the above object of the invention, the embodiment of the present invention uses the following technical solution：
A kind of efficient liquid phase detection method of l-tyrosine, chromatographic condition are as follows：
Chromatographic column：Octadecylsilane chemically bonded silica chromatographic column；
Mobile phase：The mixed solution of phosphate buffer and methanol；The volume ratio of the phosphate buffer and methanol is 6
: 3.5~4.5；
Detection wavelength：220~230nm；
Column temperature is 25~35 DEG C；
Flow rate of mobile phase：0.7~1.0mL/min.
Chromatographic condition provided by this method can make sample chromatogram peak and impurity peaks reach good separating effect, Neng Gouzhun
Really determine the content of l-tyrosine in sample to be tested, even for mixing sample, this method still can in l-tyrosine into
The accurate quantitative detection of row, detection sensitivity is high, detection limit is low, convenient for process analysis and control containing l-tyrosine product
System.When sample size is more, high performance liquid chromatography of the present invention can detecte multiple samples within the same time
Product, and detection time is short, significantly improves the working efficiency of detection.
Preferably, the phosphate buffer be the potassium dihydrogen phosphate containing 4.1g/L, 2.8ml/L triethylamine it is water-soluble
Liquid.The solution is used for mobile phase, can enable l-tyrosine that can be able to good reservation on a column.Triethylamine is made
For a kind of buffer salt, the pH for keeping phosphate buffer can be assisted to stablize, moreover it is possible in conjunction with the silicone hydroxyl of stationary phase, hinder
The effect of sample medium alkaline compound and silicone hydroxyl, to reduce the hangover of sample to be tested neutral and alkali component.
Preferably, the volume ratio of the phosphate buffer and methanol is 6: 4.It can be obtained under the ratio more perfect
Peak shape.
Preferably, the Detection wavelength is 225nm.
Preferably, the column temperature is 30 DEG C.Column temperature range is close to room temperature, and being easy to control temperature makes its stabilization, and sample is at this
Separation is good in temperature range.
Preferably, the efficient liquid phase detection method of above-mentioned l-tyrosine includes the following steps：
Step a, the reference substance solution of l-tyrosine is prepared；
Step b, high performance liquid chromatography detection is carried out to the reference substance solution with the chromatographic condition, according to gained peak face
Product draws the standard curve of l-tyrosine reference substance, obtains concentration-peak area equation of linear regression of l-tyrosine reference substance；
Step c, sample to be tested is provided, the sample to be tested is detected with the chromatographic condition, the peak face that will be obtained
Product substitutes into the concentration-peak area equation of linear regression, calculates the content of l-tyrosine in the sample to be tested.
In terms of existing technologies, the present invention uses in the operating procedure of high performance liquid chromatography, the processing side of sample
Method is simple, operation is convenient, can be improved working efficiency；It is detected, is not needed using valuableness using high performance liquid chromatography
Instrument.
Specifically, l-tyrosine reference substance solution described in above-mentioned steps a is at least 5 parts, and concentration range is 50~150
μ g/mL, solvent are purified water.L-tyrosine in 0 DEG C of water solubility be 200 μ g/ml, and the increase of solubility with temperature and rise
Height, therefore select purified water as solvent, it may insure that l-tyrosine reference substance can sufficiently dissolve in preferred concentration range.
Purified water can be miscible with arbitrary proportion with mobile phase of the invention, and the purified water within the scope of sample volume will not be to mobile phase
Property have an impact, therefore using purified water as the solvent of standard solution.
Sample to be tested described in above-mentioned steps c is dissolved with purified water before detection, avoids introducing other impurities to experiment knot
Fruit generates interference.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples, in attached drawing：
Fig. 1 is the reference substance reference substance solution high-efficient liquid phase chromatogram of the embodiment of the present invention 1；
Fig. 2 is the product to be tested solution high-efficient liquid phase chromatogram of the embodiment of the present invention 1.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Embodiment 1
The embodiment of the invention provides a kind of efficient liquid phase detection method of l-tyrosine, the high performance liquid chromatographs of use
For Agilent 1100, chromatographic column is Perkin C18 column (5 μm, 4.6*250mm), and sampling volume is 20 μ l (quantitative loop).Including
Following steps：
1, l-tyrosine control is prepared by the concentration of 50 μ g/ml, 80 μ g/ml, 100 μ g/ml, 120 μ g/ml, 150 μ g/ml
Product solution；
2, the reference substance solution is detected with high performance liquid chromatography, chromatographic condition is：
Chromatographic column：Octadecylsilane chemically bonded silica chromatographic column；
Mobile phase：The potassium dihydrogen phosphate for weighing 4.1g is dissolved in 500ml purified water, adds 2.8ml triethylamine, mixes ultrasound,
With 0.22um organic membrane filter, then plus 500ml purified water, mix, phosphate buffer obtained, by phosphate buffer and methanol
By 6：4 volume ratio be mixed evenly to get；
Detection wavelength：225nm；
Column temperature：30℃；
Flow rate of mobile phase：1.0mL/min.
20 μ l of reference substance solution injection high performance liquid chromatograph is taken to be detected, the knot of reference substance solution concentration and peak area
Fruit is shown in Table 1.The theoretical cam curve of each chromatogram is greater than 5000, and good with miscellaneous peak separating degree, and wherein concentration is 101.20 μ g/ml
Reference substance solution chromatogram it is as shown in Figure 1.
Table 1
| Reference substance actual concentrations μ g/ml | Peak area |
| 50.60 | 1998.6 |
| 80.96 | 3133.6 |
| 101.20 | 3976.0 |
| 121.44 | 4749.6 |
| 151.80 | 5872.0 |
The standard curve that l-tyrosine reference substance is drawn according to reference substance concentration and corresponding peak area, with peak area for horizontal seat
Mark, using concentration as ordinate, obtains concentration-peak area equation of linear regression of l-tyrosine reference substance：Y=0.026x-
1.2519 R2=0.9997；
3, sample to be tested is provided, 50ml is settled to purified water dissolution, then with flowing 10 times of phase dilution, molten with reference substance
Under the identical testing conditions of liquid, the sample to be tested is detected, obtaining peak area is 3951.8, by the peak area generation
Enter above-mentioned concentration-peak area equation of linear regression, the content for calculating l-tyrosine in the sample to be tested is 50.7mg.
4, precision is investigated
It takes the 20 μ l of reference substance solution that concentration is 101.20 μ g/ml to inject high performance liquid chromatograph, repeats 6 needles, gained peak
Area is respectively 3987.3,3956.5,3968.7,3976.0,3973.4,3962.9, average value 3970.8, and RSD is
0.28%.Illustrate that a kind of efficient liquid phase detection method precision of l-tyrosine provided in this embodiment is good.
5, the rate of recovery is investigated
The dissolution of sample to be tested 50.5mg purified water is settled to 50ml, with 10 times of phase dilution of flowing；By sample to be tested
50.5mg and l-tyrosine reference substance 9.36mg is settled to 50ml with purified water dissolution jointly, with 10 times of phase dilution of flowing.It will be upper
Two parts of sample feedings are stated, are detected with the identical testing conditions of reference substance solution, and record peak area.
The peak area that the sample of reference substance is added is 4689.5, and calculating content is 120.68 μ g/ml, the peak face of direct injected
Product 3976.0, calculating content are 102.12 μ g/ml, the rate of recovery 99.1%.Illustrate a kind of l-tyrosine provided in this embodiment
The efficient liquid phase detection method rate of recovery it is good.
6, quantitative limit
The detection of quantitative limit is carried out using the high performance liquid chromatograph of UV1100 model, the peak height of baseline noise is
0.03mv, according to the measuring method of quantitative limit S/N=10, quantitative limit is that peak height about injects liquid phase color in high 10 times of baseline noise
The reference substance amount of spectrometer.Reference substance 25mg/25ml is weighed, when diluting 250000 times, peak height is about 0.3mv, and reference substance is dense at this time
Degree is 0.004 μ g/ml, i.e., is quantitatively limited to 0.004 μ g/ml.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (8)
1. a kind of efficient liquid phase detection method of l-tyrosine, which is characterized in that chromatographic condition is as follows：
Chromatographic column：Octadecylsilane chemically bonded silica chromatographic column；
Mobile phase：The mixed solution of phosphate buffer and methanol；The volume ratio of the phosphate buffer and methanol is 6: 3.5
~4.5；
Detection wavelength：220~230nm；
Column temperature is 25~35 DEG C；
Flow rate of mobile phase：0.7~1.0mL/min.
2. the efficient liquid phase detection method of l-tyrosine according to claim 1, which is characterized in that the phosphate-buffered
Liquid is the aqueous solution of the potassium dihydrogen phosphate containing 4.1g/L, 2.8ml/L triethylamine.
3. the efficient liquid phase detection method of l-tyrosine according to claim 2, which is characterized in that the phosphate-buffered
The volume ratio of liquid and methanol is 6: 4.
4. the efficient liquid phase detection method of l-tyrosine according to claim 1, which is characterized in that the Detection wavelength is
225nm。
5. the efficient liquid phase detection method of l-tyrosine according to claim 1, which is characterized in that the column temperature is 30
℃。
6. the efficient liquid phase detection method of described in any item l-tyrosine according to claim 1~5, which is characterized in that including
Following steps：
Step a, the reference substance solution of l-tyrosine is prepared；
Step b, high performance liquid chromatography detection is carried out to the reference substance solution with the chromatographic condition, is drawn according to gained peak area
The standard curve of l-tyrosine reference substance processed obtains concentration-peak area equation of linear regression of l-tyrosine reference substance；
Step c, sample to be tested is provided, the sample to be tested is detected with the chromatographic condition, the peak area generation that will be obtained
Enter the concentration-peak area equation of linear regression, calculates the content of l-tyrosine in the sample to be tested.
7. the efficient liquid phase detection method of l-tyrosine according to claim 6, which is characterized in that L- described in step a
Tyrosine reference substance solution is at least 5 parts, and concentration range is 50~150 μ g/mL, and solvent is purified water.
8. the efficient liquid phase detection method of l-tyrosine according to claim 6, which is characterized in that described in step c to
Sample is dissolved with purified water before detection.
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