CN108893431A - A kind of bacterium and its application with high anti-oxidation function - Google Patents
A kind of bacterium and its application with high anti-oxidation function Download PDFInfo
- Publication number
- CN108893431A CN108893431A CN201810799319.3A CN201810799319A CN108893431A CN 108893431 A CN108893431 A CN 108893431A CN 201810799319 A CN201810799319 A CN 201810799319A CN 108893431 A CN108893431 A CN 108893431A
- Authority
- CN
- China
- Prior art keywords
- paenibacillus
- guilin
- oxidation
- guilinensis
- oxidation function
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Present invention firstly discovers that the bacterial strain of Paenibacillus bacillus genus has anti-oxidation function.The present invention isolates a Bacillus species, is verified by experiments, which has anti-oxidation function, can be used as the function stem of Oxidative Stress, its excellent antioxidant genes is transferred in other organisms, receptor can be made to obtain excellent degeneration-resistant border character.
Description
Technical field
The invention belongs to microorganisms technical field, in terms of being related to a kind of Guilin series bacillus as anti-oxidation function bacterial strain
Application.
Background technique
The change of the intracorporal redox state of biology all may cause toxic effect.If being had noxa by internal and external environment
Element stimulation can cause the macromolecular or a variety of of the excessively high perhaps Scavenging ability decline damage biological body of free-radical contents
Cellular component, such as cause protein oxidation, DNA mutation etc..
The bacterial strain with antioxygenic property is screened, its DNA damage repair ability is studied, and then be transformed into plant, helps
In cultivating the plant with anti-adversity, to adapt to extraneous continually changing climatic environment.
At present in the reported bacterial strain with oxidative stress resistance, has no and be related to Paenibacillus class gemma bar
The report of the bacterial strain of Pseudomonas.
Summary of the invention
The purpose of the present invention is be found to have the microorganism of high anti-oxidation function.
The inventors discovered that a kind of high anti-oxidation Guilin series bacillus；
The title of the bacterium is：Guilin series bacillus (Paenibacillus guilinensis H1-7)；
Deposit number：GDMCC No.60328.
High anti-oxidation Guilin series bacillus (Paenibacillus guilinensis H1-7) morphology of the invention,
Physiology and biochemistry is identified and entrusts Guangdong Province's Microbiological Culture Collection compared with nearly edge reference culture carries out Microbiological Characteristics
Center identification, the following are expert's conclusions：
High anti-oxidation Guilin of the present invention series bacillus (Paenibacillus guilinensis H1-7)
Flagellum picture under the electron microscope of Morphological Identification is as shown in Figure 1；
High anti-oxidation Guilin of the present invention series bacillus (Paenibacillus guilinensis H1-7)
There were significant differences for Microbiological Characteristics：
Comparing bacterial strain is nearly 53270 He of edge reference culture Paenibacillus vulneris CCUG of bacillus genus
Paenibacillus yunnanensis YN2；
Comparative experiments is shown：Present invention discover that high anti-oxidation Guilin series bacillus (Paenibacillus
Guilinensis H1-7) there were significant differences (table 1) with the Microbiological Characteristics of two above bacterial strain.
Table 1 and nearly edge strain microorganism Property comparison
Note："+" indicates positive, and "-" indicates negative
The identification of high anti-oxidation Guilin series bacillus anti-oxidation function
Guilin series bacillus (Paenibacillus guilinensis H1-7) of the invention is Paenibacillus
The gram-positive bacteria of category has good oxidation resistance：
1) in 20mM H2O2After impacting 10min, bacterial strain H1-7 is grown in the presence of reduction to a certain extent, and control strain
Escherichia coli (E.coli K12) do not grow (Fig. 2) then completely；
2) using Deinococcus radiodurans Deinococcus radiodurans as positive control, Guilin series bacillus
(Paenibacillus guilinensis H1-7) can be in 40mM H2O2Handle 10min and 30mM H2O2After handling 30min
Still grow (Fig. 3).
It is good to show that Guilin series bacillus of the invention (Paenibacillus guilinensis H1-7) has
Aoxidize resistance.
The category is rarely reported with anti-oxidation function.By the research to its mechanism, can further deepen people for
The understanding of anti-oxidation function mechanism of production constructs new genetically modified organism, it is made to obtain other merits.Guilin class gemma
Bacillus (the Paenibacillus guilinensis H1-7) species new as one, by for the cultivation of anti-oxidant crop and
The excavation of new antioxidant genes provides new resource.
Therefore, Guilin series bacillus of the invention (Paenibacillus guilinensis H1-7), can be direct
As anti-oxidation function bacterial strain, its excellent antioxidant genes is transferred in other organisms, allows to obtain excellent resist
Inverse character；Meanwhile it may also be obtained more as new engineering strain by receiving external other excellent genes
More merits；In addition, carrying out the improvement of character by traditional mutation breeding means, more Optimalities can also be obtained
The bacterial strain of shape, while it aoxidizes resistance mechanism, promotes new DNA technique in environmental protection, biological prosthetic, Ren Leijian to research
The development important in inhibiting of health etc..
Detailed description of the invention
Flagellum figure under the Guilin Fig. 1 series bacillus (Paenibacillus guilinensis H1-7) electron microscope
Piece (flagellum is elongated, hollow).
Fig. 2, Fig. 3 are utilized respectively 10mM-40mM H2O2Guilin series bacillus after processing 10min and 30min
(Paenibacillus guilinensis H1-7) and control strain Escherichia coli (E.coli K12) and radiation hardness exception ball
The oxidation resistance of bacterium (Deinococcus radiodurans) compares.
Specific embodiment
The oxidation resistance assay of 1 Guilin series bacillus of embodiment (Paenibacillus guilinensis H1-7)
1, test method
(1) Guilin series bacillus of the present invention (Paenibacillus guilinensis H1-7) glycerol is protected
Hide the activation of pipe TGY plate streaking, 37 DEG C of inversion cultures；
(2) bacterium colony on picking plate, is inoculated into TGY fluid nutrient medium, and 37 DEG C, 200rpm, shaking flask culture overnight；
(3) OD is measured600, according to purpose OD600=0.1~0.2, it transfers in fresh TGY fluid nutrient medium, 37 DEG C, 200
Rpm, culture to OD600It is 0.6~0.8；
(4) it takes 100 μ L bacterium solutions to be added in 900 μ L PBS and is used as untreated samples, successively gradient dilution 10-1、10-2、10-3、
10-4、10-5、10-6For use；
(5) it takes 1mL bacterium solution (to take 5 pipes) in EP pipe, under the conditions of being protected from light, sequentially adds H2O2, so that final concentration of 0mM,
10mM, 20mM, 30mM, 40mM handle 10min and 30min respectively under the conditions of being protected from light；
(6) after 10min and 30min each sample of rapid gradient dilution to 10-1、10-2、10-3、10-4、10-5、
10-6；
(7) 8 μ L of point sample on plate, 37 DEG C of inversion cultures after plate is dried.
(8) simultaneously respectively with OD600Bacterial strain is as negative control for=0.6 Escherichia coli (E.coli K12), with OD600=
0.6 Deinococcus radiodurans D.radiodurans R1 is positive control, parallel laboratory test is carried out, in point on solid medium
Plate observation compares clump count to determine its oxidation resistance.Experimental bacteria and control bacterium have respectively carried out 3 parallel tests.
2, result
The result shows that bacterial strain Guilin of the present invention series bacillus (Paenibacillus guilinensis H1-7) exists
20mM H2O2After impacting 10min, bacterial strain H1-7 is grown in the presence of reduction to a certain extent, and control strain Escherichia coli
(E.coli K12) then cannot grow (Fig. 2, Fig. 3) completely, at the same using Deinococcus radiodurans D.radiodurans R1 as
Positive control, Guilin series bacillus (Paenibacillus guilinensis H1-7) can be in 40mM H2O2Handle 10min
With 30mM H2O2It is still grown after processing 30min, shows Guilin series bacillus (Paenibacillus of the invention
Guilinensis H1-7) there is preferable oxidation resistance.
Claims (3)
1. function stem of the Guilin series bacillus (Paenibacillus guilinensis H1-7) as Oxidative Stress
Application.
2. application described in claim 1 is the genetically modified organism that building has anti-oxidation function.
3. application described in claim 1 is the application in terms of the anti-oxidation function character of mutation breeding and improvement plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810799319.3A CN108893431A (en) | 2018-07-19 | 2018-07-19 | A kind of bacterium and its application with high anti-oxidation function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810799319.3A CN108893431A (en) | 2018-07-19 | 2018-07-19 | A kind of bacterium and its application with high anti-oxidation function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108893431A true CN108893431A (en) | 2018-11-27 |
Family
ID=64351318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810799319.3A Pending CN108893431A (en) | 2018-07-19 | 2018-07-19 | A kind of bacterium and its application with high anti-oxidation function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108893431A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20170001397A (en) * | 2015-06-26 | 2017-01-04 | 충남대학교산학협력단 | Novel Paenibacillus sp. RMKS 72, fermented ginseng using the same and composition having anti-oxidant activity comprising extract of fermented ginseng using the same |
| CN108865945A (en) * | 2018-07-19 | 2018-11-23 | 中国农业科学院生物技术研究所 | Herbicide-resistant 2,4-D Guilin series bacillus and its application |
-
2018
- 2018-07-19 CN CN201810799319.3A patent/CN108893431A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20170001397A (en) * | 2015-06-26 | 2017-01-04 | 충남대학교산학협력단 | Novel Paenibacillus sp. RMKS 72, fermented ginseng using the same and composition having anti-oxidant activity comprising extract of fermented ginseng using the same |
| CN108865945A (en) * | 2018-07-19 | 2018-11-23 | 中国农业科学院生物技术研究所 | Herbicide-resistant 2,4-D Guilin series bacillus and its application |
Non-Patent Citations (4)
| Title |
|---|
| RAZA ET AL.: "Optimization,purification,characterization and antioxidant activity of an extracellular polysaccharide produced by Paenibacillus polymyxa SQR-21", 《BIORESOURCE TECHNOLOGY》 * |
| WANG ET AL.: "Production and characterization of exopolysaccharides and antioxidant from Paenibacillus sp. TKU023", 《NEW BIOTECHNOLOGY》 * |
| 赵文鹏: "类芽孢杆菌B28抗氧化物质paenibactin的分离提纯、活性及结构鉴定", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
| 陈金苹: "类芽孢杆菌ZC01胞外多糖的制备及活性研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bodhankar et al. | Maize seed endophytic bacteria: dominance of antagonistic, lytic enzyme-producing Bacillus spp. | |
| CN111254093B (en) | Bacillus belgii 229-15 and application thereof | |
| Xu et al. | Isolation and characterization of Bacillus subtilis strain 1-L-29, an endophytic bacteria from Camellia oleifera with antimicrobial activity and efficient plant-root colonization | |
| Deepa et al. | Plant growth-promoting activity in newly isolated Bacillus thioparus (NII-0902) from Western ghat forest, India | |
| CN106148480A (en) | A kind of super resistance to selenium antibacterial research method to the reduction of selenite | |
| CN103571770B (en) | A kind of Efficient peanut rhizobiumleguminosarstrain strain and application thereof | |
| CN107893047B (en) | Petroleum aromatic hydrocarbon degrading bacterium and application thereof | |
| Ahmed | How Chromium-Resistant Bacteria Can Improve Corn Growth in Chromium-Contaminated Growing Medium. | |
| Padden et al. | Indigo-reducing Clostridium isatidis isolated from a variety of sources, including a 10th-century viking dye vat | |
| Tran et al. | Characterization of bacteria type strain Bacillus. spp isolated from extracellular polymeric substance harvested in seafood wastewater | |
| Khatun et al. | Estimation of heavy metal tolerance and antibiotic susceptibility of Bacillus cereus isolated from municipal solid waste | |
| Ragavi et al. | Phenotypic and molecular characterization of endophytic bacteria isolated from banana | |
| Ahmed et al. | Diversity of Rhizobium leguminosarum bv. Viceae strains isolated from different schemes in Shendi area | |
| Poonam et al. | Bacterial degradation and decolorization of textile dyes by newly isolated Lysobacter sp. | |
| Wafula et al. | Isolation and characterization of bacillus species from soil in Ngere tea catchment area of Murang’a county, Kenya | |
| CN108410778B (en) | Method for quickly and efficiently separating bacterial ear blight bacteria of rice | |
| CN108893431A (en) | A kind of bacterium and its application with high anti-oxidation function | |
| Zhang et al. | Development of a strain-specific quantification method for monitoring Bacillus amyloliquefaciens TF28 in the rhizospheric soil of soybean | |
| RU2488630C1 (en) | STRAIN Micrococcus luteus, HAVING CATALASE ACTIVITY AND PERFORMING TRANSFORMATIONS OF ORGANIC REMAINS OF NATURAL ORIGIN | |
| Altaf et al. | In vitro biofilm development and enhanced rhizosphere colonization of Triticum aestivum by fluorescent Pseudomonas sp | |
| Deepthi et al. | In vitro study on the effect of heavy metals on PGPR microbes from two different soils and their growth efficiency on Oryza sativa (L.). | |
| CN107217016A (en) | One plant has the endophytic Bacillus bacterial strain ZY122 for suppressing rice sheath blight disease and its application | |
| George et al. | Isolation, Identification and Characterisation of Endophytic Bacteria in Biophytum sensitivum (L.) DC | |
| Ramachandiran et al. | Identification and characterization of gut associated bacteria in Epilachna vigintioctopunctata Fab.(Coleoptera: Coccinellidae) | |
| Javed et al. | Isolation, molecular identification and characterization of boron-tolerant bacterial strains from sewage treatment pond of Islamabad, Pakistan |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |