CN108872226A - A kind of reagent of quick colorimetric detection nitrate anion and nitrite anions - Google Patents
A kind of reagent of quick colorimetric detection nitrate anion and nitrite anions Download PDFInfo
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- CN108872226A CN108872226A CN201811000105.1A CN201811000105A CN108872226A CN 108872226 A CN108872226 A CN 108872226A CN 201811000105 A CN201811000105 A CN 201811000105A CN 108872226 A CN108872226 A CN 108872226A
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- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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Abstract
The present invention provides a kind of reagents of quick colorimetric detection NO3-N and NO2-N, the reagent is made of amino benzenes compounds and strong acid, detection reagent of the present invention shows high selectivity to NO3-N and NO2-N, to ensure that the nitrate anion that can be directed in the non-standard explosive of trace and nitrite anions of the invention are detected.3 s reach Glassless discoloration after this reagent is contacted with nitrate anion and nitrite anions, without complicated analytical equipment, to realize for nitrate anion and the inexpensive, instant of nitrite anions, trace detection.In addition, the preparation of reagents is easy, it is easy to operate, high sensitivity, it is at low cost, overcome it is in the prior art can not direct color comparison detection nitrate anion, the deficiencies of sensitivity is low, the reaction time is long, in the non-standard explosive of trace detection nitrate anion and nitrite anions effective technical support is provided.
Description
Technical field
The invention belongs to non-standard explosive detection field, a kind of quick colorimetric detection nitrate anion and nitrite anions are provided
Reagent.
Background technique
Nearly attack of terrorism in 20 years takes place frequently, and has seriously threatened the safety of life and property (Analytica of the global people
chimica acta,2015,893:1-13；Nanoscale, 2016,8：1305–1308；Forensic science
international,2014,242:228-235).Rather than standard explosive, since its is cheap, raw material is easy to get, be not easy by
Detection and become terrorist preferred manner (Talanta, 2016,161：219–227).Non- standard explosive is typically based on nothing
Machine high energy oxidizer such as nitrate, chlorate or perchlorate form (TrAC Trends in Analytical
Chemistry,2014,56:27-36).And nitrate (ammonium nitrate, potassium nitrate, sodium nitrate etc.) is used as most common agricultural fertilizer,
It is mixed with any fuel (gasoline, diesel oil, sugar, charcoal, flour etc.) can very easily prepare explosive (Aspects
of explosives detection.Elsevier,2011).Famous Boston marathon case of explosion is exactly to utilize within 2013
Black powder (potassium nitrate, sulphur and charcoal) carries out explosive attack activity, causes 3 people's death and more than 170 people injury (Forensic
Science International, 2014,242：228–235).In addition, find according to the statistics made by the departments concerned, it is most vehicle-mounted
Explosive attack be all using ammonium nitrate explosive, including appalling Oklahoma City's explosive attack event (Defence
Today,2008,4649:46-49).Notorious " Irish Republican Army " is mixed using ammonium nitrate and candy, in Northern Ireland
With London manufactured a large amount of attack of terrorism (Science and Justice, 2009,49:73–80).In addition, Spain
Ammonium nitrate-stone is widely used in terroristic organization's Etard (ETA), Colombian Revolutionary Armed Forces (FARC) and Palestinian extremist
Oil carry out attack of terrorism activity (Applied Magnetic Resonance, 2012,43:557-566).More it has been reported that
Nitrite anions can be used as a kind of non-standard explosive oxidant constituents (Aspects of Explosives Detection,
2009,11-26).Therefore, scientific research personnel develops many detection methods for nitro explosive.Such as, Malaysian university
M.R.Mahmoudian seminar successfully realize the detection to nitrate anion using electrochemical method, and its detection limit down to
0.4535 μM (Journal of Electroanalytical Chemistry, 2015,751：30–36).Plymouth, Britain
The Paul J.Worsfold seminar of university realizes the detection for nitrate anion and nitrite anions using chemiluminescent method
Limit respectively 0.02 μ g N/L and 0.02 μ g N/L (Luminescence, 2012,27.5:419-425).Japan Kagoshima university
Hitoshi Kodamatani seminar using liquid chromatography realize to river water, pond water, rainwater, commercial mineral water and
Nitrate anion and nitrite anions in tap water detection (Journal of Chromatography A, 2009,1216:3163-
3167).The Shin-ichi Wakida seminar of state-run industrial technology comprehensive study institute of Japan utilizes capillary electrophoresis successfully
Realize detection (the Journal of Chromatography limited nitrite and nitrate detection down to 2.6 and 1.5 μM
A,2004,1051:185-191).The Andreas K Nussler seminar of Berlin, Germany University Hospital utilizes 2,3- diamino
Naphthalene can be reacted with nitrite anions to form 2,3- naphthalene nitre azoles and generating stronger fluorescence phenomenon realizes fluorescence to nitrite anions
Detection (Nature Protocols, 2006,1,2223-2226).The Jianguo Li seminar of University Of Suzhou utilizes water solubility
CdSe quantum dot Electrochemiluminescince successfully realize to nitrite anions detection (Luminescence, 2013,28:551–
556).In numerous detection methods, spectral technique is widely used because its detection limit is low and easy to operate, including ultraviolet-can
See analysis (Analytica chimica acta, 1994,299:81-90), chemiluminescence analysis (Fresenius'Journal
of Analytical Chemistry,2000,367.3:264-269.), fluorescence analysis (Analytical Letters,
2000,33:1869-1883), infrared spectroscopy (Journal of Plant Nutrition, 2000,23:79-90), Raman light
Spectrum (The Journal of Chemical Physics, 1999,110:2202-2207), molecule vacancy emission spectrum
(Analyst,1989,114.5:563-566).However these are using the method for chemical reaction detection nitrate anion, including the most often
Griess reagent is all by first detecting reducing nitrate radical again for the stronger nitrite anions of chemical activity
(Analytica chimica acta,1995,308:413-424).It is found according to extensive literature survey, only Israel is special
The Michael Gozin seminar of La Weifu university utilizes under molybdenum-copper catalysis system, and nitrate anion can be by sulfide oxidation at sulfoxide
Characteristic, be successfully realized fluorescence method directly detect nitrate anion purpose (Organic letters, 2011,13:5532-
5535).And colorimetric method is because its is easy to operate, visual result is reliable and becomes the most promising method of detection field.So
And regrettably, up to the present, there are no people directly to be detected to nitrate anion class with the method for chemical colorimetric.For
It can be realized the field quick detection analysis to suspicious nitrate anion class explosive, developing one kind being capable of direct color comparison detection nitric acid
The reagent of root becomes the world-famous puzzle for being badly in need of solving and most have challenge.
Based on this, the present invention develops the reagent of a kind of quick colorimetric detection nitrate anion and nitrite anions.The reagent is main
The nitrification ability of nitrate anion is improved using strong acid, and nitrate anion is more likely to nitrify the contraposition of strong electron-donating group, therefore chooses
A kind of amino benzenes compounds with strong electron-donating group, generate the nitration product of brown color.To reach quick colorimetric detection
The purpose of nitrate anion.In addition, this reagent it is quick, it is sensitive, highly selective and easy to operate, be conducive to promote and apply.
Summary of the invention
Present invention aims at provide the reagent of a kind of quick colorimetric detection nitrate anion and nitrite anions, the reagent is by benzene
Aminated compounds, strong acid and ultrapure water are made, and detection reagent of the present invention shows nitrate anion and nitrite anions extremely low
Detection limit (open hole detection limits 10 μM) and high selectivity, this guarantees the present invention can be for non-standard explosion raw material
In nitrate anion and nitrite anions ingredient detected.The reagent changes colour immediately after contacting with nitrate anion and nitrite anions, is not necessarily to
Complicated analytical equipment, to realize for nitrate anion and the inexpensive, instant of nitrite anions, trace detection.Reagent behaviour
Make simplicity, high sensitivity is at low cost.Overcome spy used in colorimetric detection nitrate anion in the prior art and nitrite anions method
The deficiencies of needle molecule synthesis is complicated, sensitivity is low, the reaction time is long provides effective skill for non-standard explosion raw material detection field
Art means.
The reagent of a kind of quick colorimetric detection nitrate anion and nitrite anions of the present invention, which is by phenyl amines
Conjunction object, which is dissolved in the ultra-pure water solution of a certain concentration strong acid, to be made, wherein：
Amino benzenes compounds are that N- benzoyl replaces aniline, 2', 5'- diethoxy benzanilide, 3- amino -4- methoxy second
Oxygroup acetanil, 4,4'- diaminobenzene anilid or monoacetylaniline, and the content of amino benzenes compounds in the solution
For 10mg/L-40g/L；
Strong acid is perchloric acid, hydroiodic acid, hydrochloric acid or sulfuric acid, and the concentration of strong acid is 30%-98%；
Solvent is ultrapure water；
It is dissolved in the strong acid that ultrapure water diluted, stirs evenly using by amino benzenes compounds, time 20-30min, i.e.,
Obtain the reagent of colorimetric detection nitrate anion and nitrite anions.
The reagent is applied to nitric acid by the reagent of a kind of quick colorimetric detection nitrate anion and nitrite anions of the present invention
The specific method is as follows for the colorimetric detection of root and nitrite anions：
Prepare detection reagent：
Weigh 0.1mg, 0.2mg, 0.4mg, 0.6mg, 0.8mg, 1mg, 2mg, 4mg, 6mg, 8mg, 10mg, 40mg, 70mg,
The amino benzenes compounds of 100mg and 400mg are dissolved in the strong acid that ultrapure water diluted, that is, obtaining concentration is 10mg/L, 20mg/
L、40mg/L、60mg/L、80mg/L、100mg/L、200mg/L、400mg/L、600mg/L、800mg/L、1g/L、4g/L、7g/
L, aniline-strong acid solution of 10g/L and 40g/L；
By aniline-strong acid solution of various concentration with ultrapure water be diluted to strong acid concentration be 30%, 35%, 40%,
45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% and 98% to get arrive phenyl amines chemical combination
The concentration of object is 10mg/L-40g/L, and strong acid concentration is the detection reagent of 30%-98%；
The detection reagent of preparation is applied to the colorimetric detection of nitrate anion and nitrite anions：
Weigh KNO3Be dissolved in ultrapure water, be configured to nitrate anion concentration be 1M, 900mM, 800mM, 700mM,
600mM、500mM、400mM、300mM、200mM、100mM、90mM、80mM、70mM、60mM、50mM、40mM、30mM、20mM、
10mM、9mM、8mM、7mM、6mM、5mM、4mM、3mM、2mM、1mM、900μM、800μM、700μM、600μM、500μM、400μM、
300 μM, 200 μM, 100 μM, 90 μM and 80 μM of aqueous solution；
Weigh NaNO2Be dissolved in ultrapure water, be configured to nitrite anions concentration be 1M, 900mM, 800mM, 700mM,
600mM、500mM、400mM、300mM、200mM、100mM、90mM、80mM、70mM、60mM、50mM、40mM、30mM、20mM、
10mM、9mM、8mM、7mM、6mM、5mM、4mM、3mM、2mM、1mM、900μM、800μM、700μM、600μM、500μM、400μM、
300 μM, 200 μM and 100 μM of aqueous solution；
The detection reagent for measuring 300 μ L with liquid-transfering gun is separately added into nitrate anion or the Asia of 3 μ L various concentrations in 96 orifice plates
Nitrate solutions scan the color image of reaction front and back after 3s with flat bed scanner, measure the naked eye recognition detection limit of reagent；Together
When, comparison reaction front and back color change takes the Euclidean distance matched curve of RGB；
It is wiped respectively with sampling paper and takes micro KNO3、NaNO3、NH4NO3、Zn(NO3)2、NaNO2、NH4NO2、KClO3With
NH4Cl, 1-2 drop detection reagent in drop are observed color change, are determined whether containing nitrate or nitrite.
This reagent not only can detecte nitrate or nitrite in liquid, can also detect micro solid nitric acid salt
Or remnant nitrite.In addition, this reagent has excellent selectivity for common inorganic salts chaff interferent.
The reagent of a kind of quick colorimetric detection nitrate anion and nitrite anions of the present invention explodes mainly for non-standard
Nitrate anion and nitrite anions ingredient in object are detected, solve currently can not for nitrate anion ingredient carry out directly, quickly,
Visualization, low cost detection problem.
Compared with prior art, the beneficial effects of the invention are as follows：
1. reagent raw material used in the present invention can be commercially available by commercial market；
2. the detection NO3-N and NO2-N that detection reagent of the invention can be sensitive, highly selective；
3. detection reagent rapid reaction of the invention, 3s can be realized to nitrate anion and nitrite detection；
4. detection reagent detection nitrate anion of the invention and nitrite anions can be directly naked without complicated analytical equipment
Eye recognition detection；
5. determinand without being carried out any pre-processing by detection reagent of the invention, easy to operate, popularization is facilitated to answer
With the detection of this reagent limits low, strong interference immunity and the reaction time is short, and low cost, real-time colorimetric detection nitric acid may be implemented completely
The purpose of root and nitrite anions.
Detailed description of the invention
It for concentration of the aniline in 50% perchloric acid is 10mg/L- that Fig. 1, which is flat bed scanner of the present invention scanning N- benzoyl,
The detection reagent and 10mM KNO of 40g/L3The color change and reaction front and back color for reacting front and back subtract each other gained picture；
It for concentration of the aniline in 50% perchloric acid is 10mg/L-40g/ that Fig. 2, which is RGB data of the present invention processing N- benzoyl,
The detection reagent and 10mM KNO of L3Color change curve after reaction；
Fig. 3 is that the 3- amino -4- methoxyethoxy acetanil of RGB data of the present invention processing 4g/L is dissolved in 50%-
The KNO of gained reagent detection 10mM in the sulfuric acid of 98% concentration3Gained map, wherein illustration is the examination of flat bed scanner Scanning Detction
The color change and reaction front and back color of agent reaction front and back subtract each other gained picture；
Fig. 4 is that the 4,4'- diaminobenzene anilid of flat bed scanner of the present invention scanning 0.1g/L is dissolved in 50% hydrogen iodine
The KNO for the reagent detection 0-10mM being formulated in acid3Gained picture；
Fig. 5 is that the 4,4'- diaminobenzene anilid of RGB data of the present invention processing 0.1g/L is dissolved in 50% hydroiodic acid
Gained reagent detects the KNO of 0.8 μM of -10mM3Gained map；
Fig. 6 is dissolved in 30% hydrochloric acid for the monoacetylaniline that flat bed scanner of the present invention scans 0.01g/L and is formulated
Reagent detection 0-10mM NaNO2Gained picture；
Fig. 7 is that the monoacetylaniline of RGB data of the present invention processing 0.01g/L is dissolved in gained reagent detection in 30% hydrochloric acid
The NaNO of 1 μM of -10mM2Gained map.
Specific embodiment
Below by specific embodiment, the present invention is described further, but invention is not limited to these embodiments.
Embodiment 1
At room temperature, weigh 0.1mg, 0.2mg, 0.4mg, 0.6mg, 0.8mg, 1mg, 2mg, 4mg, 6mg, 8mg, 10mg,
The N- benzoyl of 40mg, 70mg, 100mg and 400mg are dissolved in respectively in the perchloric acid that 10mL concentration is 70% for aniline, stirring
Uniformly obtain the reagent of quick colorimetric detection nitrate anion and nitrite anions；
By the KNO of prepared various concentration reagent and 1M3The flat-bed scanning figure of front and back is reacted as shown in Figure 1, can by Fig. 1
To find out：Reagent before reaction gradually becomes buff from colourless as N- benzoyl replaces the increase of concentration of aniline, and reacts
Solution afterwards gradually becomes brown color from faint yellow；In order to intuitively compare the variation of reaction front and back color, reaction is utilized
Color afterwards, which is directly subtracted each other, reacts shown in 1 third of the picture row of preceding color, it can be seen that：After reaction with react before solution dyeing
As N- benzoyl is gradually brownish black by blue black discoloration for the increase of concentration of aniline；And further after reaction and instead
The Euclidean distance for answering preceding solution colour is ordinate, and N- benzoyl is that abscissa mapping can be seen that for concentration of aniline：N- benzene first
The concentration range of anilid can will become apparent from shown in the variation diagram 2 of Euclidean distance in 10mg/L-40g/L.
Embodiment 2
At room temperature, 98% concentrated sulfuric acid is diluted to 50% with ultrapure water, 55%, 60%, 65%, 70%, 75%, 80%,
85%, 90% and 95%, the 3- amino -4- methoxyethoxy acetanil for then weighing 40mg is dissolved in 10mL various concentration
Sulfuric acid in, stir evenly to get arrive quick colorimetric detection nitrate anion and nitrite anions reagent；
By the KNO of prepared different sulfuric acid concentration reagents and 1M3React flat-bed scanning figure such as Fig. 3 (illustration) institute of front and back
Show, it can be seen that：Reagent before reaction gradually becomes faint yellow from colourless with the increase of sulfuric acid concentration, when sulfuric acid concentration is
Color reaches most deep when 70%, is then gradually become colorless by faint yellow with the increase of sulfuric acid concentration；And the solution after reacting
Gradually become brown color from faint yellow, then gradually becomes faint yellow from brown color with the increase of sulfuric acid concentration；In order to
The further variation of comparison reaction front and back solution colour, using the Euclidean distance of reaction front and back as ordinate, the concentration of sulfuric acid is
Abscissa maps (as shown in Figure 3), it can be seen that：Sulfuric acid concentration is evident that the change of Euclidean distance in 50%-98%
Change.
Embodiment 3
At room temperature, 57% hydroiodic acid is diluted to 50% with ultrapure water, 4, the 4'- diamino benzoyl for then weighing 1mg replaces
Aniline is dissolved in 50% hydroiodic acid of 10mL, stirs evenly the examination for obtaining quick colorimetric detection nitrate anion and nitrite anions
Agent；
By prepared detection reagent and 10mM, 9mM, 8mM, 7mM, 6mM, 5mM, 4mM, 3mM, 2mM, 1mM, 900 μM, 800 μ
M、700μM、600μM、500μM、400μM、300μM、200μM、100μM、90μM、80μM、70μM、60μM、50μM、40μM、30μ
M, 20 μM, 10 μM, 9 μM, 8 μM, 7 μM, 6 μM, 5 μM, 4 μM, 3 μM, 2 μM, 1 μM, 0.9 μM, 0.8 μM and 0 μM of KNO3Ultrapure water
It is as shown in Figure 4 must to react front and back color change with flat-bed scanning for solution reaction；As seen from Figure 4：With KNO3The increasing of concentration
Solubilization liquid gradually becomes yellow from colourless, works as KNO3Concentration be 10 μM can naked eye find out color change；In order to be more accurate
The variation of comparison reaction front and back solution colour, using the Euclidean distance before and after reacting as ordinate, KNO3Concentration be abscissa make
Scheme (as shown in Figure 5), it can be seen that：With KNO3The increase Euclidean distance of concentration gradually increases；Fig. 5 by Euclidean distance with
KNO3Concentration variation carry out exponential fitting can be seen that：Euclidean distance and KNO3Concentration shows extraordinary exponential relationship, from
And utilize Euclidean distance to KNO3Carry out quantitative analysis.
Embodiment 4
At room temperature, 38% hydrochloric acid is diluted to 30% with ultrapure water, the monoacetylaniline for then weighing 0.1mg is dissolved in
In 30% hydrochloric acid of 10mL, the reagent for obtaining quick colorimetric detection nitrate anion and nitrite anions is stirred evenly；
By prepared detection reagent and 10mM, 9mM, 8mM, 7mM, 6mM, 5mM, 4mM, 3mM, 2mM, 1mM, 900 μM, 800 μ
M、700μM、600μM、500μM、400μM、300μM、200μM、100μM、90μM、80μM、70μM、60μM、50μM、40μM、30μ
M, 20 μM, 10 μM, 9 μM, 8 μM, 7 μM, 6 μM, 5 μM, 4 μM, 3 μM, 2 μM, 1 μM and 0 μM of NaNO2Ultra-pure water solution reaction, is used
Flat-bed scanning reaction front and back color change is as shown in Figure 6；As seen from Figure 6：With NaNO2The increase solution of concentration gradually by
It is colourless to become yellow；The variation of reaction front and back solution colour is compared in order to be more accurate, is to react the Euclidean distance of front and back
Ordinate, NaNO2Concentration be abscissa map (as shown in Figure 7), it can be seen that：With NaNO2The increase Euclidean distance of concentration
It gradually increases；Fig. 7 by Euclidean distance with NaNO2Concentration variation carry out exponential fitting can be seen that：Euclidean distance with
NaNO2Concentration shows extraordinary exponential relationship, so as to utilize Euclidean distance to NaNO2Carry out quantitative analysis.
Embodiment 5
At room temperature, the perchloric acid that concentration is 70% is diluted to 60% with ultrapure water, then weighs the N- benzoyl of 0.4mg
It is dissolved in 60% perchloric acid of 10mL for aniline, stirs evenly the examination for obtaining quick colorimetric detection nitrate anion and nitrite anions
Agent；
Micro KNO is wiped with sampling paper3After solid residue, after sampling paper center dropwise addition 1-2 drop detection reagent, 3s
This it appears that：Sampling paper center quickly can become yellow from colourless；It proves former containing non-standard explosive in object to be checked
Expect nitrate.
Embodiment 6
At room temperature, concentration is diluted to 40% with ultrapure water for 57% hydroiodic acid, then weighs the 2' of 0.1mg, 5'- diethyl
Oxygroup benzanilide is dissolved in 40% hydroiodic acid of 10mL, is stirred evenly and is obtained quick colorimetric detection nitrate anion and nitrous
The reagent of acid group；
Micro NaNO is wiped with sampling paper3After solid residue, 1-2 drop detection reagent, 3s is added dropwise at sampling paper center
Afterwards this it appears that：Sampling paper center quickly can become yellow from colourless, it was demonstrated that contain non-standard explosive in object to be checked
Raw material nitrate.
Embodiment 7
At room temperature, the hydrochloric acid that concentration is 38% is diluted to 30% with ultrapure water, then weighs 4, the 4'- diamino of 6mg
Benzanilide is dissolved in 38% hydrochloric acid of 10mL, is stirred evenly and is obtained quick colorimetric detection nitrate anion and nitrite anions
Reagent；
Micro NH is wiped with sampling paper4NO3After solid residue, 1-2 drop detection reagent, 3s is added dropwise at sampling paper center
Afterwards this it appears that：Sampling paper center quickly can become yellow from colourless, it was demonstrated that contain non-standard explosive in object to be checked
Raw material nitrate.
Embodiment 8
At room temperature, concentration is diluted to 75% with ultrapure water for 98% concentrated sulfuric acid, then weighs the 3- amino -4- first of 8mg
Oxygen Ethoxyacetyl is dissolved in 75% sulfuric acid of 10mL for aniline, is stirred evenly and is obtained quick colorimetric detection nitrate anion and Asia
The reagent of nitrate anion；
Micro Zn (NO is wiped with sampling paper3)2After solid residue, 1-2 drop detection reagent is added dropwise at sampling paper center,
After 3s this it appears that：Sampling paper center quickly can become yellow from colourless, it was demonstrated that explode in object to be checked containing non-standard
Raw material nitrite.
Embodiment 9
At room temperature, concentration is diluted to 60% with ultrapure water for 70% perchloric acid, then weighs the monoacetylaniline of 10mg
It is dissolved in 60% perchloric acid of 10mL, stirs evenly the reagent for obtaining quick colorimetric detection nitrate anion and nitrite anions；
Micro NaNO is wiped with sampling paper2After solid residue, 1-2 drop detection reagent, 3s is added dropwise at sampling paper center
Afterwards this it appears that：Sampling paper center quickly can become yellow from colourless, it was demonstrated that contain non-standard explosive in object to be checked
Raw material nitrite.
Embodiment 10
At room temperature, the hydrochloric acid that concentration is 38% is diluted to 35% with ultrapure water, then weighs the 2' of 0.4mg, 5'- diethyl
Oxygroup benzanilide is dissolved in 35% hydrochloric acid of 10mL, is stirred evenly and is obtained quick colorimetric detection nitrate anion and nitrous acid
The reagent of root；
Micro NH is wiped with sampling paper4NO2After solid residue, 1-2 drop detection reagent, 3s is added dropwise at sampling paper center
Afterwards this it appears that：Sampling paper center quickly can become yellow from colourless, it was demonstrated that contain non-standard explosive in object to be checked
Raw material nitrate.
Embodiment 11
At room temperature, the hydroiodic acid that concentration is 57% is diluted to 55% with ultrapure water, then weighs the 3- amino-of 0.2mg
4- methoxyethoxy acetanil is dissolved in 55% hydroiodic acid of 10mL, is stirred evenly and is obtained quick colorimetric detection nitric acid
The reagent of root and nitrite anions；
Micro KClO is wiped with sampling paper3After solid residue, 1-2 drop detection reagent, 3s is added dropwise at sampling paper center
Post-sampling paper center is without any color change, it was demonstrated that without containing non-standard explosion raw material nitrate or nitrous acid in object to be checked
Salt.
Embodiment 12
At room temperature, the hydroiodic acid that concentration is 57% is diluted to 45% with ultrapure water, then weighs the N- benzoyl of 0.6mg
It is dissolved in 45% hydroiodic acid of 10mL for aniline, stirs evenly the examination for obtaining quick colorimetric detection nitrate anion and nitrite anions
Agent；
Micro NH is wiped with sampling paper4After Cl solid residue, 1-2 drop detection reagent, 3s is added dropwise at sampling paper center
Post-sampling paper center is without any color change, it was demonstrated that without containing non-standard explosion raw material nitrate or nitrous acid in object to be checked
Salt.
Although above embodiments describe the present invention, it should be appreciated that in the premise without prejudice to spirit of the invention
Under, the reagent component ratio in the present invention can be transferred, and it is equally applicable for the hypochlorite test in other fields, and these
Variation also belongs to the scope of the present invention.
Claims (1)
1. a kind of reagent of quick colorimetric detection nitrate anion and nitrite anions, it is characterised in that the reagent by amino benzenes compounds,
Strong acid and ultrapure water are made, wherein：
Amino benzenes compounds are that N- benzoyl replaces aniline, 2', 5'- diethoxy benzanilide, 3- amino -4- methoxyethoxy
Acetanil, 4,4'- diaminobenzene anilid or monoacetylaniline, the content of amino benzenes compounds in the solution are
10mg/L-40g/L；
Strong acid is perchloric acid, hydroiodic acid, hydrochloric acid or sulfuric acid, and the concentration of strong acid is 30%-98%；
Solvent is ultrapure water；
It is dissolved in the strong acid that ultrapure water diluted, stirs evenly using by amino benzenes compounds, time 20-30min is to get arriving
The reagent of colorimetric detection nitrate anion and nitrite anions.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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