CN108872162A - A kind of analysis method of the detection carcinomebryonic antigen optical electro-chemistry aptamers based on heterojunction composite - Google Patents
A kind of analysis method of the detection carcinomebryonic antigen optical electro-chemistry aptamers based on heterojunction composite Download PDFInfo
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Abstract
The analysis method for the detection carcinomebryonic antigen optical electro-chemistry aptamers based on heterojunction composite that the invention discloses a kind of.G-C is synthesized first3N4/Bi2MoO6Heterojunction photovoltaic material, g-C3N4/Bi2MoO6Heterojunction photovoltaic material is supported on hetero-junctions surface by acting on capture gold nano modification aptamers nano-probe with π-π, finally using this compound as the photo electric sensitive element of detection CEA.At low cost in the photoelectricity DNA sensing that the present invention constructs, environmentally friendly, stability is good, and preparation process is simple, high sensitivity.
Description
Technical field
The present invention relates to optical electro-chemistry aptamers field of sensing technologies, are based on heterojunction composite more particularly to one kind
Detection carcinomebryonic antigen optical electro-chemistry aptamers analysis method.Specifically g-C3N4/Bi2MoO6Heterojunction photovoltaic material is base
Bottom material acts on capture gold nano by π-π and modifies CEA aptamers nano-probe, in this, as a kind of detection carcinomebryonic antigen
Optical electro-chemistry aptamers analysis method.
Background technique
Carcinoembryonic Antigen CEA is a kind of tumor associated antigen, when CEA apparent increase common colon cancer, gastric cancer, lung cancer, bile duct
Cancer；Also there is raising in liver cancer, breast cancer, oophoroma, cancer of pancreas.CEA detection after monitoring treatment with blood circulation CEA for holding
Continuing raised patient has critically important value, can prompt latent transfer and Residual Disease.
Currently, the detection method most common for carcinomebryonic antigen is chemoluminescence method and ELISA method.But these methods need
Certain instrument and equipment is wanted, its preparation process makes testing cost higher because needing enzyme, and detection time is long, is suitable for
The detection of a large amount of samples in self-contained laboratory.Optical electro-chemistry sensing technology is different from traditional Electroanalytical Chemistry, due to swashing
Hair energy and detection signal adhere to two different form of energy separately, therefore have higher sensitivity.Surface plasma resonance
（SPR）The physical basis of sensing technology is a kind of quantum photoelectric phenomenon as caused by light incidence metal surface.SPR effect is main
It is the accuracy height of measurement, fast response time, body including Localized field enhancement, photo-thermal effect and thermoelectronic effect the method advantage
Small and strong antijamming capability of product etc..
Summary of the invention
Based on background above, the purpose of the present invention is to provide a kind of detection carcinomebryonic antigen based on heterojunction composite
The analysis method of optical electro-chemistry aptamers.Its technical principle is to act on capture gold nano modification by π-π under excited by visible light
The composite material g-C of CEA aptamers nano-probe3N4/Bi2MoO6The absorption spectrum of light, nanometer gold surface local electricity can be expanded
Field intensity enhancing, decays with post plasma, and the conduction band of the energy transfer of aggregation to composite material, photoelectric current enhances.
Under ultraviolet excitation, the passivation of gold nano active surface, local electric field intensity is weak, and illumination is prevented to be mapped to composite material surface,
Photoelectric current reduces.
To achieve the above object, the technical solution adopted by the present invention is as follows：
A kind of analysis method of the detection carcinomebryonic antigen optical electro-chemistry aptamers based on heterojunction composite includes the following steps：
(1) g-C3N4Monomer preparation：1.0-5.0 g dicyanodiamines are weighed in crucible, are then put in Muffle furnace high temperature
Calcining, temperature is 100-800 DEG C, and keeps the temperature 1-6 h at this temperature；To after reaction, crucible cooled to room temperature
Afterwards, it is ground to powder, for use；
(2) composite material g-C3N4/Bi2MoO6Preparation：Weighing mass ratio is 1：8 g-C3N4 With Bi (NO3)5·5H2O is dissolved in
In deionized water, under constant stirring, Na is added2MoO4·2H2O forms white precipitate, persistently stirs 30 min；It will be above-mentioned outstanding
Turbid shifts in the reaction kettle of polytetrafluoroethylene (PTFE), and 6-24 h is heated at 160 DEG C；After reaction, it is naturally cooled to reaction kettle
Room temperature, gained yellow mercury oxide by for several times centrifugation, deionized water and dehydrated alcohol washing removal product in remaining ion, after
Sample is collected in dry 12 h, grinding at 60 DEG C.
(3) preparation of gold nano grain Au NPs：HAuCl4·6H2The water of 100ml is added in O, is heated to boiling, add
Trisodium citrate heats 15min, is cooled to room temperature；Wherein HAuCl4·6H2The mass ratio of O and trisodium citrate is 1：5.
(4) preparation of gold nano modification CEA aptamers nano-probe：CEA aptamers stoste and three (2- carboxyethyl) phosphines
The mass ratio of the material 1:100 mixing 1 hour；Again by 10 μ L, 10.0 μM of adaptation liquid solutions are added to 200 μ L Au NPs solution
In, 16 h are reacted at room temperature, and close nanogold with 1.0 wt % bovine serum albumin(BSA)s；Centrifugal dispersion is in PBS buffer solution
In.
(5) by composite material g-C3N4/Bi2MoO6, gold nano modifies CEA aptamers nano-probe and CEA is incubated for, point
Photocurrent response is not detected under ultraviolet light and visible light：Take 1-10 mgmL-1g-C3N4/Bi2MoO6Hetero-junctions is dripped in conduction
On the surface glass FTO, then take gold nano modification aptamers DNA chain nano-probe drop in above-mentioned FTO, respectively ultraviolet and can
To various concentration standard sample test light electric current under light-exposed irradiation；It is drawn according to the concentration of standard items and the relationship of photo-current intensity
Standard working curve, sample photo-current intensity corresponding concentration value on standard working curve are that the measurement of sample to be tested is dense
Degree.
Step（2）The composite material g-C3N4/Bi2MoO6In preparation, g-C3N4And Bi2MoO6Mass ratio be
25%, Bi (NO3)5·5H2O and Na2MoO4·2H2The molar ratio of O is 1:4, the reaction time is 6-24 hours.
Step（5）The ultraviolet light is the injection light of 365 nm, it is seen that light is the injection light of 550 nm.
Advantages of the present invention is as follows：
1 composite nano materials g-C provided by the present invention3N4/Bi2MoO6Raw material is easy to get, and preparation process is simple, low in cost.
Surface plasma resonance effect combination optical electro-chemistry is utilized in 2 present invention, can be detected at room temperature extremely low
The Carcinoembryonic Antigen CEA of concentration shows highly sensitive, stability height, the good feature of selectivity.
3 aptamer sensor structures and preparation process of the present invention are very simple, low in cost, before having a wide range of applications
Scape.
4 provide a kind of new approaches for the development of optical electro-chemistry sensing.
Detailed description of the invention
Fig. 1 composite material g-C3N4/Bi2MoO6Transmission electron microscope picture；
Fig. 2 composite material g-C3N4/Bi2MoO6X-ray photoelectron spectroscopy figure；
The analysis method schematic diagram of detection carcinomebryonic antigen optical electro-chemistry aptamers of the Fig. 3 based on heterojunction composite
The photoelectric response characteristic of the analysis method of detection carcinomebryonic antigen optical electro-chemistry aptamers of the Fig. 4 based on heterojunction composite
Curve；Wherein figure A is the photoelectric response characteristic curve under visible light, and figure B is the photoelectric response characteristic curve under ultraviolet light, schemes C
For the photoelectric respone standard working curve under visible light；Scheming D is the photoelectric respone standard working curve under ultraviolet light.
Specific embodiment
Below by specific implementation example, technical scheme is described further, but this cannot be limited with this
The range of invention.
Embodiment 1
1. g-C3N4Preparation
It weighs 2.0 g dicyanodiamines to be placed in crucible, and 550 DEG C of 4 h of calcining is heated to 1.0 DEG C/min in Muffle furnace.
To after reaction, after crucible cooled to room temperature, be ground to powder, for use.
2.g-C3N4/Bi2MoO6The preparation of compound
Weigh 0.5 g g-C3N4It is distributed to containing 2 mmol Bi (NO3)5·5H2In the 25 mL distilled water of O.It is being vigorously stirred feelings
Under condition, by 0.121g Na2MoO4·2H2O is added in above-mentioned solution, and obtained solution is then transferred to poly- the four of 50 mL
Hydro-thermal reaction 12 hours at 160 DEG C are heated in vinyl fluoride.After band naturally cools to room temperature, yellow solid production is collected by centrifugation
Object is washed three times respectively with water and ethyl alcohol, dry.G-C can be seen from Fig. 13N4And Bi2MoO6It is combined with each other well,
Enhance the separation of electron hole.
3. the preparation of gold nano grain Au NPs
While stirring, HAuCl4·6H2O (1 mL, 1%;W/w) be added into the secondary water of 100 mL, then plus
The sodium citrate of 2.5 mL 1% (w/w), 15 min of continuous heating are added into above-mentioned mixed liquor to boiling for heat.Color is from sallow
Become claret, be then centrifuged for separating, is saved at 4 DEG C.
4. the preparation of nanogold and aptamers nano-probe compound
CEA aptamers stoste and three (2- carboxyethyl) phosphines press the mass ratio of the material 1:100 mixing 1 hour.Above-mentioned adaptation liquid solution
(10 μ L, 10.0 μM) are added to 200 μ L Au NPs solution and react 16 h at room temperature, and use bovine serum albumin(BSA)
(1.0 wt %) closes nanogold.5 min are centrifuged under 10 000 rpm revolving speeds, and are scattered in (10 in PBS buffer solution
mM, pH 7.4)。
5. optical electro-chemistry aptamers analysis method detects CEA
Fig. 3 is the signal of the analysis method of the detection carcinomebryonic antigen optical electro-chemistry aptamers the present invention is based on heterojunction composite
Figure.Weigh the g-C of above-mentioned preparation3N4/Bi2MoO61 mg mL of compound-1Uniformly it is coated onto 28.26 mm of FTO effective area2, room temperature
It is dry.10 μ L gold nanos and aptamers nano-probe compound are added on above-mentioned electrode, after being incubated for 1 hour, are buffered with PBS
Liquid washs 3 times.Secondly, a series of CEA standard sample of 10 μ L is added is incubated for 1 h at 37 DEG C, washed 4 times with PBS buffer solution.
The electrode prepared detects photoelectricity using three-electrode system at ultraviolet (365 nm) and visible light (550 nm) irradiation respectively
Stream.Standard working curve is drawn according to the relationship that photocurrent values and CEA concentration change, as shown in Figure 4.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (9)
1. a kind of analysis method of the detection carcinomebryonic antigen optical electro-chemistry aptamers based on heterojunction composite：Its feature exists
In：G-C is synthesized first3N4/Bi2MoO6Heterojunction photovoltaic material, g-C3N4/Bi2MoO6Heterojunction photovoltaic material with π-π by making
It is supported on hetero-junctions surface with capture gold nano modification aptamers nano-probe, finally using this compound as detection carcinomebryonic antigen
The photo electric sensitive element of CEA.
2. a kind of detection carcinomebryonic antigen optical electro-chemistry aptamers based on heterojunction composite according to claim 1
Analysis method：It is characterized in that：Specifically include following steps：
(1) g-C3N4Monomer preparation；
(2) heterojunction photovoltaic material g-C3N4/Bi2MoO6Preparation.
(3) preparation of gold nano grain Au NPs；
(4) gold nano grain of preparation is mixed with mercapto-modified aptamers DNA chain, preparation gold nano modifies CEA aptamers
Nano-probe；
(5) by composite material g-C3N4/Bi2MoO6, gold nano modifies CEA aptamers nano-probe and CEA is incubated for, respectively in purple
Photocurrent response is detected under outer light and visible light.
3. a kind of detection carcinomebryonic antigen optical electro-chemistry aptamers based on heterojunction composite according to claim 2
Analysis method：It is characterized in that：The heterojunction photovoltaic material g-C3N4/Bi2MoO6Preparation includes the following steps：
(1) g-C3N4Monomer preparation：The dicyanodiamine of 1.0 ~ 5.0 g is weighed in crucible, Muffle furnace high temperature is then put in and forges
It burns, and keeps the temperature 1-6 h at this temperature；To after reaction, after crucible cooled to room temperature, be ground to powder, for use；
(2) g-C is weighed3N4 With Bi (NO3)5·5H2O is dissolved in deionized water, under constant stirring, Na is added2MoO4·
2H2O forms white precipitate, persistently stirs 30 min；In the reaction kettle that above-mentioned suspension is shifted to polytetrafluoroethylene (PTFE), at 160 DEG C
Heat 6-24 h；After reaction, to reaction kettle cooled to room temperature, gained yellow mercury oxide is by centrifugation, deionization for several times
Remaining ion in water and dehydrated alcohol washing removal product, after at 60 DEG C dry 12 h, grinding collect sample.
4. analysis method according to claim 3, it is characterised in that：Bi (the NO3)5·5H2O and Na2MoO4·
2H2The molar ratio of O is 1:4.
5. analysis method according to claim 3, it is characterised in that：Step（1）Described in calcination temperature be 100-800
℃。
6. analysis method according to claim 3, it is characterised in that：Step（2）Middle g-C3N4 With Bi (NO3)5·5H2O
Mass ratio is 1:8.
7. a kind of detection carcinomebryonic antigen optical electro-chemistry aptamers based on heterojunction composite according to claim 2
Analysis method：It is characterized in that：The step（3）The preparation of gold nano grain Au NPs is specially：HAuCl4·6H2O is added
The water of 100ml is heated to boiling, and adds trisodium citrate, heats 15min, is cooled to room temperature；Wherein HAuCl4·6H2O with
The mass ratio of trisodium citrate is 1：5.
8. a kind of detection carcinomebryonic antigen optical electro-chemistry aptamers based on heterojunction composite according to claim 2
Analysis method：It is characterized in that：The step（4）Specially：CEA aptamers stoste and three (2- carboxyethyl) phosphine substances
Measure ratio 1:100 mixing 1 hour；Again by 10 μ L, 10.0 μM of adaptation liquid solutions are added in 200 μ L Au NPs solution, in room
Temperature is lower to react 16 h, and closes nanogold with 1.0 wt % bovine serum albumin(BSA)s；Centrifugal dispersion is in PBS buffer solution.
9. a kind of detection carcinomebryonic antigen optical electro-chemistry aptamers based on heterojunction composite according to claim 2
Analysis method：It is characterized in that：The step（5）Specially：Take 1-10 mgmL-1g-C3N4/Bi2MoO6Hetero-junctions drop exists
On the surface electro-conductive glass FTO, then take gold nano modification aptamers DNA chain nano-probe drop in above-mentioned FTO, respectively ultraviolet
With under radiation of visible light to various concentration standard sample test light electric current；According to the relationship of the concentration of standard items and photo-current intensity
Standard working curve is drawn, sample photo-current intensity corresponding concentration value on standard working curve is the survey of sample to be tested
Determine concentration.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111693585A (en) * | 2020-06-29 | 2020-09-22 | 大连工业大学 | Semiconductor nano biosensor and preparation method thereof |
| CN111912887A (en) * | 2020-06-22 | 2020-11-10 | 江苏中江材料技术研究院有限公司 | Preparation method of photoelectrochemical ofloxacin aptamer sensor based on hydrotalcite/graphite-like phase carbon nitride heterojunction |
| CN112213499A (en) * | 2020-10-12 | 2021-01-12 | 闽江学院 | Beta-catenin photoelectric immune analysis method based on Z-type tungsten trioxide-nanogold-indium zinc sulfide heterojunction |
| CN114199956A (en) * | 2021-11-02 | 2022-03-18 | 东莞理工学院 | Photoelectric sensor for detecting human prostate specific antigen by immune reaction |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105126919A (en) * | 2015-08-24 | 2015-12-09 | 福州大学 | Compound visible photocatalyst, and preparation method and application thereof |
| CN105738352A (en) * | 2016-02-25 | 2016-07-06 | 济南大学 | Preparing method and application of photoelectrochemistry ractopamine sensor based on bi-metal co-doped two-dimensional manometer material |
| CN107262131A (en) * | 2017-07-17 | 2017-10-20 | 河海大学 | A kind of visible light-responded Bi3O4Cl/g‑C3N4The preparation method and application of heterojunction material |
-
2018
- 2018-05-09 CN CN201810438016.9A patent/CN108872162B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105126919A (en) * | 2015-08-24 | 2015-12-09 | 福州大学 | Compound visible photocatalyst, and preparation method and application thereof |
| CN105738352A (en) * | 2016-02-25 | 2016-07-06 | 济南大学 | Preparing method and application of photoelectrochemistry ractopamine sensor based on bi-metal co-doped two-dimensional manometer material |
| CN107262131A (en) * | 2017-07-17 | 2017-10-20 | 河海大学 | A kind of visible light-responded Bi3O4Cl/g‑C3N4The preparation method and application of heterojunction material |
Non-Patent Citations (3)
| Title |
|---|
| HAIPING LI等: "Synthesis and characterization of g-C3N4/Bi2MoO6 heterojunctions with enhanced visible light photocatalytic activity", 《APPLIED CATALYSIS B: ENVIRONMENTAL》 * |
| TINGTING WU等: "Label-free photoelectrochemical immunosensor for carcinoembryonic antigen detection based on g-C3N4 nanosheets hybridized with Zn0.1Cd0.9S nanocrystals", 《SENSORS AND ACTUATORS B》 * |
| ZAN DAI等: "Crystal defect engineering of Aurivillius Bi2MoO6 by Ce doping for increased reactive species production in photocatalysis", 《AMERICAN CHEMICAL SOCIENTY》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111912887A (en) * | 2020-06-22 | 2020-11-10 | 江苏中江材料技术研究院有限公司 | Preparation method of photoelectrochemical ofloxacin aptamer sensor based on hydrotalcite/graphite-like phase carbon nitride heterojunction |
| CN111912887B (en) * | 2020-06-22 | 2023-02-17 | 江苏中江材料技术研究院有限公司 | Preparation method of photoelectrochemical ofloxacin aptamer sensor based on hydrotalcite/graphite-like phase carbon nitride heterojunction |
| CN111693585A (en) * | 2020-06-29 | 2020-09-22 | 大连工业大学 | Semiconductor nano biosensor and preparation method thereof |
| CN111693585B (en) * | 2020-06-29 | 2022-08-02 | 大连工业大学 | Semiconductor nano biosensor and preparation method thereof |
| CN112213499A (en) * | 2020-10-12 | 2021-01-12 | 闽江学院 | Beta-catenin photoelectric immune analysis method based on Z-type tungsten trioxide-nanogold-indium zinc sulfide heterojunction |
| CN114199956A (en) * | 2021-11-02 | 2022-03-18 | 东莞理工学院 | Photoelectric sensor for detecting human prostate specific antigen by immune reaction |
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