CN108865738A - One kind splitting formula out and quantitatively easily marks anti-skidding criss-cross method surface sampling culture dish - Google Patents

Abstract

Formula out, which is split, the invention discloses one kind quantitatively easily marks anti-skidding criss-cross method surface sampling culture dish, the culture dish includes ware bottom and ware lid, it can split set on the transversal crack mouth of the first ware downside wall and longitudinal crack mouth when stressed, it is detached from movable ware downside wall, the height of the first ware downside wall is caused to be lower than the plane of solid medium, therefore the operation of surface microorganism sampling is easier, when multiple culture dishes are stacked placement, first protrusion and the second protrusion can limit the relative movement of two adjacent culture dish, mark zone can make markers work more efficient, what graduation mark can indicate agar medium pours into volume, grid line can simplify the counting process of bacterium colony.

Description

One kind splitting formula out and quantitatively easily marks anti-skidding criss-cross method surface sampling culture dish

Technical field

Splitting formula out the present invention relates to a kind of experiment equipment, especially one kind quantitatively easily marks anti-skidding criss-cross method surface to sample Culture dish.

Background technique

Culture dish is one of most widely used experiment equipment in biology, is usually used in the objects such as packaging material for food, tableware The culture of body surface microorganism is tested, and the sampling of body surface microorganism usually wipes determinand body surface with sterile cotton swab Cotton swab or filter paper are perhaps transferred in buffer with aseptic filter paper abutting object under test surface later and are eluted, to reach by face To the purpose of sampling.But cotton swab or filter paper can not discharge microorganism collected completely, be easy to cause Bacterial diversity Incomplete culture；In addition the sampling of body surface microorganism needs sterile working, and cotton swab or filter paper are carrying out microorganism Acquisition and elution during, be easy pollution by environmental microorganism, be extremely difficult to the requirement of sterile working；These disadvantages It will cause the deviation of body surface microorganism testing result.In addition, the operating process of the above method is cumbersome, inefficiency, hold Easily cause the waste of human resources.Existing culture dish is mostly glass material, need to could be marked with permanent pen, but oiliness The person's handwriting of marking pen need to could be wiped with special organic solvent, therefore when multiple culture dishes are reused, the not side of label Just it be easy to cause and obscures.When the culture dish pours into agar medium, culture medium pour into volume be usually operator empirically Depending on, therefore the agar medium of different personnel pours into volume and may have larger difference, to influence relevant experimental result.When When the colony counts for the target culture that culture medium in culture dish is grown are huge, the counting process of bacterium colony is then comparatively laborious.

Summary of the invention

For disadvantages mentioned above, the purpose of the present invention is to provide a kind of structure be simple and convenient to operate to split formula out quantitative easy Mark anti-skidding criss-cross method surface sampling culture dish.The implementation of the culture dish can simplify the sampling of body surface microorganism Journey improves the accuracy of test result, improves working efficiency.

Anti-skidding criss-cross method surface sampling culture dish is easily quantitatively marked for splitting formula out, to reach the purpose, the present invention is adopted Technical solution is：One kind splitting formula out and quantitatively easily marks anti-skidding criss-cross method surface sampling culture dish, including ware bottom and relaxation The ware lid being covered on ware bottom, it is characterised in that：The ware lid includes tegmental wall and at least one surround the ware holded up from tegmental wall Lid side wall, the ware bottom includes bottom wall and at least one surround the first ware downside wall holded up from bottom wall；First ware Downside wall is equipped at least one circle transversal crack mouth, and the transversal crack choma was around described first ware downside wall one week, the cross Circle plane to crackle mouth is parallel with the plane of the bottom wall；The first ware downside wall is by the transversal crack mouth point It is divided into movable ware downside wall and fixed ware downside wall；The interconnecting piece of the movable ware downside wall and the fixation ware downside wall It is divided into transversal crack wall, the thickness of the transversal crack wall is less than the first ware downside wall；The movable ware downside wall is equipped with extremely A few longitudinal crack mouth perpendicular to the transversal crack mouth, the movable ware downside wall of longitudinal crack mouth two sides pass through vertical It is connected to crackle wall, the thickness of the longitudinal crack wall is less than the first ware downside wall；The diameter of the ware lid side wall is greater than described First ware downside wall；The diameter of the bottom wall is greater than the tegmental wall；The height of the activity ware downside wall and the fixed ware bottom The sum of height of side wall, less than the height of the ware lid side wall；The bottom wall is additionally provided at least one first protrusion, the tegmental wall It is additionally provided at least one second protrusion；In the outer surface of the tegmental wall, it is equipped at least one mark zone, the mark zone is by covering Wall surface is formed through frosted processing；The first ware downside wall is additionally provided at least one graduation mark, and the graduation mark is surround First ware downside wall one week, the circular flat of the graduation mark is parallel with the plane of the bottom wall；In the bottom wall, it is equipped with cross Bottom wall is divided into the region of several squares or approximating square, institute by the grid line of perpendicular orthogonal crossover, the grid line State the area equation of square area.

Compared with the prior art, the beneficial effects of the invention are as follows：The longitudinal crack wall and transversal crack wall of the culture dish Thickness very little, be easy to rupture under external force, when applying force to the movable ware downside wall, the first ware bottom can be made Side wall splits at longitudinal crack mouth and transversal crack mouth, with removal activity ware downside wall, and then makes the height of the first ware downside wall Degree decreases below the plane of solid medium, and the solid culture base plane is directly covered in determinand body surface when use Face can reach the purpose of sampling microorganisms from surface of object.Therefore the structure of the culture dish is simple, easy to use, Ke Yijian The process for changing sampling microorganisms from surface of object overcomes the problems, such as to be easy to be polluted by environmental microorganism in sampling process, improve simultaneously Test result accuracy and working efficiency, when multiple culture dishes are stacked placement, the first protrusion and the second protrusion can To limit the relative movement of two adjacent culture dish, mark zone can make markers work more efficient, and graduation mark can indicate that agar is trained That supports base pours into volume, and grid line can simplify the counting process of bacterium colony.

Detailed description of the invention

Illustrate technical solution of the present invention in order to clearer, it below will be to attached drawing needed in embodiment description Make brief introduction, it should be apparent that, drawings in the following description are only some embodiments of the invention, general for this field For logical technical staff, without creative efforts, it can also be obtained according to these attached drawings other attached drawings.

Fig. 1 is to split the quantitative structural schematic diagram for easily marking anti-skidding criss-cross method surface sampling culture dish of formula out；Fig. 2 is provided at The schematic diagram of the grid line of the bottom wall；Fig. 3 is the cross-sectional view of the movable ware downside wall；Fig. 4 is first ware The partial enlargement diagram of downside wall；Respectively number is respectively in figure：

1 is ware lid, and 2 be ware bottom, and 3 be tegmental wall, and 4 be bottom wall, and 5 be ware lid side wall, and 7 be the first ware downside wall, and 71 be movable ware bottom Side wall, 72 be transversal crack mouth, and 73 be transversal crack wall, and 74 be longitudinal crack mouth, and 75 be longitudinal crack wall, and 76 be fixed ware bottom Side wall, 10 be the second protrusion, and 11 be the first protrusion, and 20 be frosted mark zone, and 22 be graduation mark, and 23 be grid line.

Specific embodiment

It is right below in conjunction with the drawings and specific embodiments in order to be more clear the objectives, technical solutions, and advantages of the present invention The present invention is described in further detail.It should be appreciated that described embodiment is only used to explain the present invention, it is all to be based on Simplified or equivalent variations, all belongs to the scope of protection of the present invention made by technical solution of the present invention.

In the discussion of specific embodiment, the formula out of splitting quantitatively easily marks anti-skidding criss-cross method surface sampling culture dish Shape preferably selects circle, and still, those skilled in the art should be understood that it is suitable for such as oval Shape, rectangle or other shapes suitable for culture dish.

Formula out, which is split, the invention discloses one kind quantitatively easily marks anti-skidding criss-cross method surface sampling culture dish, including ware bottom 2 The ware lid 1 being covered on ware bottom 2 with relaxation, the ware lid 1 includes tegmental wall 3 and at least one surround the ware holded up from tegmental wall 3 Lid side wall 5, the ware bottom 2 includes bottom wall 4 and at least one surround the first ware downside wall 7 holded up from bottom wall 4；Described One ware downside wall 7 is equipped at least one circle transversal crack mouth 72, and the transversal crack mouth 72 is around the first ware downside wall 7 one Week, the circle plane of the transversal crack mouth 72 are parallel with the plane of the bottom wall 4；The first ware downside wall 7 is described Transversal crack mouth 72 be divided into movable ware downside wall 71 and fixed ware downside wall 76；The movable ware downside wall 71 with it is described Fixation ware downside wall 76 coupling part be transversal crack wall 73, the thickness of the transversal crack wall 73 is less than the first ware bottom side Wall 7；The movable ware downside wall 71 be equipped at least one perpendicular to the transversal crack mouth 72 longitudinal crack mouth 74, it is described The movable ware downside wall 71 of 74 two sides of longitudinal crack mouth is connected by longitudinal crack wall 75, and the thickness of the longitudinal crack wall 75 is small In the first ware downside wall 7.Features described above has determined that the formula out of splitting quantitatively easily marks anti-skidding criss-cross method surface sampling culture dish Basic structure, the wherein thickness very little of longitudinal crack wall 75 and transversal crack wall 73 is easy to rupture under external force, makes the One ware downside wall 7 splits at longitudinal crack mouth 74 and transversal crack mouth 72, and movable ware downside wall 71 can be from fixed ware at this time It is detached from downside wall 76.And the space that the first ware downside wall 7 and bottom wall 4 are formed is injected as culture inner cavity when cultivating inner cavity When enough agar mediums（The liquid level of the culture medium is higher than the transversal crack mouth 72）, solidified to the culture medium Afterwards, the movable ware downside wall 71 is applied force to, makes the first ware downside wall 7 in longitudinal crack mouth 74 and transversal crack mouth 72 Place splits, and with removal activity ware downside wall 71, and then the height of fixed ware downside wall 76 is made to decrease below the agar culture The agar medium plane is directly covered in object under test surface when use, that is, can reach body surface by the plane of base The purpose of microbe sampling avoids and carries out the tedious steps eluted again after microorganism acquisition, pole using cotton swab or filter paper Big simplify the process of sampling microorganisms from surface of object, and overcomes and be easy to be polluted by environmental microorganism in sampling process Problem, while improving test result accuracy and working efficiency.

It is split in the quantitative embodiment for easily marking anti-skidding criss-cross method surface sampling culture dish of formula out described, the bottom wall 4 Diameter be greater than the tegmental wall 3 diameter, the setting of this feature, can limit when ware lid 1 and ware bottom 2 fastening when, ware lid side wall 5 Bottom wall 4 can only be supported in.In addition, the height of the activity ware downside wall 71 and the sum of the height of the fixed ware downside wall 76, Less than the height of the ware lid side wall 5, this feature can limit activity ware downside wall 71 and lid when ware lid 1 and the fastening of ware bottom 2 There are certain gaps between wall 3, prevent the agar medium from contacting with tegmental wall 3.

The culture dish further include at least one be located at the bottom wall 4 the first protrusion 11 and at least one be located at Second protrusion 10 of the tegmental wall 3.First protrusion 11 is identical as the shape of the second protrusion 10.First protrusion 11 and the The effect of two protrusions 10 is embodied in, and when multiple culture dishes are arranged to stacked structure, the first of a culture dish is convex It plays 11 to cooperate with the second protrusion 10 of adjacent culture dish, one culture dish can be limited relative to the adjacent culture dish It is mobile.

In the outer surface of the tegmental wall 3, it is equipped at least one mark zone 20, the mark zone 20 is passed through by 3 surface of tegmental wall Frosted processing is formed；The rough surface of the mark zone 20, common pencil, wax crayon, white board marker etc. can be in mark zones 20 Apparent person's handwriting is formed, and the person's handwriting is washed with water and can easily be removed, and keeps the markers work of the culture dish more square Just efficiently.

The first ware downside wall 7 is additionally provided at least one graduation mark 22, and the graduation mark 22 is around the first ware bottom Side wall 7 one weeks, the circular flat of the graduation mark 22 was parallel with the plane of the bottom wall 4；The graduation mark 22 can indicate Agar medium pours into volume, to avoid pouring into the difference of volume because of personal experience's difference bring.

In the bottom wall 4, the grid line 23 equipped with vertical and horizontal orthogonal crossover, bottom wall 4 is divided by the grid line 23 The region of several squares or approximating square, the area equation of the square area；When the culture medium institute in culture dish When the colony counts of the target culture of growth are huge, the bacterium colony count results in a square area can be used, multiplied by phase The multiple answered（In the embodiment shown in Figure 2, the side length of the square area is 1 centimetre, agar medium in ware bottom 2 Diameter is 10 centimetres, then its area is 78.5 square centimeters, so multiple is 78.5）To indicate all clump counts of culture dish, Therefore the counting process of bacterium colony is simplified.

Above by description of listed embodiment, the basic ideas and basic principles of the present invention are elaborated.Obviously, institute The embodiment of description is only a part of the embodiments of the present invention, and non-limiting exhaustion.Based on the embodiments of the present invention, originally Field those of ordinary skill all other embodiment obtained under the premise of not making the creative labor, belongs to this hair Bright protection scope.

Claims (1)

1. one kind is split, formula out is quantitative easily to mark anti-skidding criss-cross method surface sampling culture dish, including ware bottom and relaxation to be covered in ware bottom On ware lid, it is characterised in that：The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up from tegmental wall, described Ware bottom include bottom wall and at least one from bottom wall around the first ware downside wall for holding up；The first ware downside wall is equipped with extremely Few circle transversal crack mouth, the transversal crack choma was around described first ware downside wall one week, the circle of the transversal crack mouth It is parallel with the plane of the bottom wall to enclose plane；The first ware downside wall is divided into movable ware bottom by the transversal crack mouth Side wall and fixed ware downside wall；The coupling part of the movable ware downside wall and the fixation ware downside wall is transversal crack Wall, the thickness of the transversal crack wall is less than the first ware downside wall；The movable ware downside wall be equipped at least one perpendicular to The movable ware downside wall of the longitudinal crack mouth of the transversal crack mouth, longitudinal crack mouth two sides is connected by longitudinal crack wall It connects, the thickness of the longitudinal crack wall is less than the first ware downside wall；The diameter of the ware lid side wall is greater than first ware bottom side Wall；The diameter of the bottom wall is greater than the tegmental wall；The height of the activity ware downside wall and the height of the fixed ware downside wall The sum of, less than the height of the ware lid side wall；The bottom wall is additionally provided at least one first protrusion, and the tegmental wall is additionally provided at least One second protrusion；In the outer surface of the tegmental wall, it is equipped at least one mark zone, the mark zone is by wall surfaces through grinding Sand processing is formed；The first ware downside wall is additionally provided at least one graduation mark, and the graduation mark is around the first ware bottom side Wall one week, the circular flat of the graduation mark was parallel with the plane of the bottom wall；In the bottom wall, it is equipped with vertical and horizontal orthogonal crossover Grid line, the grid line by bottom wall be divided into several square or approximating square region, the square region The area equation in domain.