CN108853539A - A kind of low temperature plasma ozone sterilizer and sterilizing methods - Google Patents
A kind of low temperature plasma ozone sterilizer and sterilizing methods Download PDFInfo
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- CN108853539A CN108853539A CN201810155576.3A CN201810155576A CN108853539A CN 108853539 A CN108853539 A CN 108853539A CN 201810155576 A CN201810155576 A CN 201810155576A CN 108853539 A CN108853539 A CN 108853539A
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- Prior art keywords
- ozone
- cavity
- solenoid valve
- low temperature
- vacuum pump
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/14—Plasma, i.e. ionised gases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
- A61L2/06—Hot gas
- A61L2/07—Steam
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
- A61L2/202—Ozone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/10—Apparatus features
- A61L2202/13—Biocide decomposition means, e.g. catalysts, sorbents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/24—Medical instruments, e.g. endoscopes, catheters, sharps
Abstract
The invention discloses a kind of low temperature plasma ozone sterilizer, including cavity, for cavity inner wall by the fixed positive electrode plate of tetrafluoro fixing piece and layer frame, positive electrode plate is electrically connected plasma power supply, cavity ground connection；Instrument is placed in low temperature plasma ozone sterilizer, closed low temperature plasma ozone sterilizer by the present invention in implementing；Evacuated pressure -30pa arrives -50pa, cavity heating is carried out, in intracavitary generation plasma discharge, water vapour and ozone are added in mixing steam case, electric discharge generates OH base by mixed vapour, to sterilize to instrument, when intracavitary air pressure is in 150pa-200pa, interruption is discharged, diffusion sterilizes, it vacuumizes the air in cavity by the intracorporal ozone ingredient of ozone destroyer breaks down chamber, pulse vacuum carries out removal water vapour repeatedly, takes object after having dried；Instead of the overlong time of existing low temperature sterilizer, concentration is high, saturating power is weak, have corrosion and residual the phenomenon that, overall structure is simply clean, sterilizing thoroughly, no dead angle.
Description
Technical field
The present invention relates to medical equipment low temperature sterilizer fields, and in particular to a kind of low temperature plasma ozone sterilizer and
Sterilizing methods.
Background technique
At present medical equipment low temperature sterilizer using ethylene oxide go out mattress device, hydrogen peroxide low-temperature plasma sterilize
Device, low temperature formaldehyde go out mattress device and traditional gas fumigation method to sterilize, and reach sterilizing purpose, however ethylene oxide goes out, mattress device sterilizes
Overlong time, hydrogen peroxide low-temperature plasma sterilizer have corrosiveness to instrument, for a long time with can have shadow to instrument life
It rings, and low temperature formaldehyde goes out mattress device and gas fumigating system, a penetration power is weak, two high using concentration, meanwhile, after the above sterilizer sterilizing
Article inside have bactericidal agent residual phenomena, once patient will be caused not using on remaining article application human body
Repairable loss, it is therefore desirable to a kind of low temperature sterilizer and method.
Summary of the invention
It is an object of the invention to overcome the above-mentioned problems in the prior art, a kind of low temperature plasma ozone is provided
Sterilizer and sterilizing methods do not have harmful substance residual, and it is thorough to sterilize.
To realize above-mentioned technical purpose and the technique effect, the present invention is achieved through the following technical solutions：
A kind of low temperature plasma ozone sterilizer, including cavity, the cavity inner wall are fixed just by tetrafluoro fixing piece
Electrode plate and layer frame, the positive electrode plate are electrically connected plasma power supply, and the cavity is grounded, deployment instrument in the layer frame；
The cavity is communicated with vacuum pump, and the vacuum pump and cavity are connected with vacuum pump solenoid valve；
The cavity has also been sequentially communicated mixing steam solenoid valve and mixing steam case, and the mixing steam case air inlet connects
Steam heating case is passed to, the steam heating case is connected to inlet valve and ozone solenoid valve, the ozone solenoid valve control
Gas in ozone storage box enters steam heating case, and the ozone storage box air inlet is sequentially communicated ozone generator and oxygen
Pneumoelectric magnet valve, the oxygen solenoid valve control oxygen enter in ozone generator；
The inside cavity is equipped with pressure sensor and ozone meter.
Further, the vacuum pump gas outlet is communicated with ozone decomposer.
Further, the positive electrode plate is mesh plate.
A kind of low temperature plasma ozone sterilization method, it is characterised in that：Include the following steps：
Step S1：Instrument is placed in layer frame, carry out being heated to after airtight cavity-degree Celsius；
Step S2：Oxygen solenoid valve is opened, oxygen generates ozone by ozone generator and enters in ozone storage box, when
When ozone concentration reaches setting concentration in ozone storage box, oxygen solenoid valve is closed；
Step S3：Pure water is entered in steam heating case by inlet valve, closes inlet valve after reaching setting water level, this
When heating steam heater box in water to-degree Celsius generate water vapour；
Step S4：Vacuum pump solenoid valve is opened, starting vacuum pump takes out negative pressure into cavity, when cavity internal pressure sensor sense
When should arrive vacuum degree and reaching -30pa to -50pa, vacuum pump solenoid valve and vacuum pump are closed；
Step S5：It opens plasma power supply and electric discharge is generated by positive electrode plate and cavity；
Step S6：Opening ozone solenoid valve enters the water vapour taken away on the water surface in steam heating case and enters mixing
In steam case, open mixing steam solenoid valve into cavity enter mixing steam, when chamber pressure reach pa-pa when interrupt etc. from
Sub- corona discharge discharges by water vapour and ozone generation-OH base, is diffused 50-65 minutes, carries out the mattress that goes out to instrument；
Step S7：It opens vacuum pump solenoid valve starting vacuum pump and takes out negative pressure into cavity, by the air in cavity by smelly
The intracorporal ozone ingredient of oxygen destroyer breaks down chamber repeats rapid step S2-S6, until apparatus sterilizing is qualified；
Step S8：It is vacuumized into cavity, greenhouse holding-degree in cavity, opens intake valve at this time and pass through high efficiency particulate air filter
Enter air to cavity after filtering, then vacuumizes, add air, remove out water vapour for several times repeatedly, it is dry to instrument
It is measured in cavity after ozone free with by ozone meter, takes out instrument after drying.
Further, in the step S6, steam solenoid valve is mixed as pulse unlatching, time and pulse number can be set,
Ozone concentration is measured by ozone meter in cavity.
Further, in the step S7, step S2-S6 in triplicate.
Income effect of the invention is：
Instrument is placed in low temperature plasma ozone sterilizer, closed low temperature plasma ozone sterilizer；It takes out true
Pneumatics power -30pa arrives -50pa, carries out cavity heating and is added to water vapour and ozone mixed in intracavitary generation plasma discharge
It closes in steam case, forms mixed vapour, to intracavitary addition mixed vapour, in this way, electric discharge generates OH base by mixed vapour, so as to right
Instrument sterilizes, and when intracavitary air pressure is in 150pa-200pa, interruption electric discharge, diffusion sterilizes, and vacuumizing will be in cavity
Air pass through the intracorporal ozone ingredient of ozone destroyer breaks down chamber.Make the return trip empty gas, and pulse vacuum carries out removal water vapour repeatedly, very
Empty drying instruments, after having dried, restoring chamber pressure sterilizing terminates, and takes object.
Instead of the overlong time of existing low temperature sterilizer, concentration is high, saturating power is weak, have corrosion and residual the phenomenon that, it is whole
Body structure is simply clean, and sterilizing is thorough, no dead angle.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, will be described below to embodiment required
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for ability
For the those of ordinary skill of domain, without creative efforts, it can also be obtained according to these attached drawings other attached
Figure.
Fig. 1 is the principle schematic diagram of sterilizer of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts all other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figure 1, the present invention is a kind of low temperature plasma ozone sterilizer, including cavity 1,1 inner wall of cavity pass through
The fixed positive electrode plate 2 of tetrafluoro fixing piece 3,4 and layer frame 6, positive electrode plate 2 are electrically connected plasma power supply 14, and cavity 1 is grounded, layer
Deployment instrument 5 on frame 6；
Cavity 1 is communicated with vacuum pump 8, and vacuum pump 8 and cavity 1 are connected with vacuum pump solenoid valve 7；
Cavity 1 has also been sequentially communicated mixing steam solenoid valve 22 and mixing steam case 21, and mixing 21 air inlet of steam case connects
Steam heating case 20 is passed to, steam heating case 20 is connected to inlet valve 19 and ozone solenoid valve 18, and ozone solenoid valve 18 is controlled
Gas in ozone storage box 17 processed enters steam heating case 20, and 17 air inlet of ozone storage box is sequentially communicated ozone generator
16 and oxygen solenoid valve 15, oxygen solenoid valve 15 controls oxygen and enters in ozone generator 16；
Pressure sensor 12 and ozone meter 13 are equipped with inside cavity 1.
One preference of present embodiment is,.
One preference of present embodiment is that 8 gas outlet of vacuum pump is communicated with ozone decomposer 9.
One preference of present embodiment is that positive electrode plate 2 is mesh plate.
A kind of low temperature plasma ozone sterilization method, includes the following steps：
Step S1：Instrument 5 is placed in layer frame 6, carries out being heated to 50-60 degrees Celsius after airtight cavity 1；
Step S2：Oxygen solenoid valve 15 is opened, oxygen generates ozone by ozone generator 16 and enters ozone storage box
In 17, when ozone concentration reaches setting concentration in ozone storage box 17, oxygen solenoid valve 15 is closed；
Step S3：Pure water is entered in steam heating case 20 by inlet valve 19, closes water inlet after reaching setting water level
Valve 19, the at this time water in heating steam heater box 20 to 60-70 degrees Celsius of generation water vapour；
Step S4：Vacuum pump solenoid valve 7 is opened, starting vacuum pump 8 takes out negative pressure into cavity 1, when cavity internal pressure senses
When device 12 senses that vacuum degree reaches -30pa to -50pa, vacuum pump solenoid valve 7 and vacuum pump 8 are closed；
Step S5：It opens plasma power supply 14 and electric discharge is generated by positive electrode plate 2 and cavity 1；
Step S6：Opening ozone solenoid valve 18 enters the water vapour taken away on the water surface in steam heating case 20 and enters
It mixes in steam case 21, opens mixing steam solenoid valve 22 and enter mixing steam into cavity 1, when chamber pressure reaches 150pa-
Plasma power supply 14 is interrupted when 200pa to discharge, and is discharged by water vapour and ozone generation-OH base, is diffused 5-10 minutes, to device
Tool carries out the mattress that goes out；
Step S7：It opens the starting vacuum pump 8 of vacuum pump solenoid valve 7 and takes out negative pressure into cavity, the air in cavity is passed through
Ozone decomposer 9 decomposes the ozone ingredient in cavity 1, rapid step S2-S6 is repeated, until apparatus sterilizing is qualified；
Step S8：It is vacuumized into cavity, greenhouse keeps 50-65 degree in cavity, opens intake valve 11 at this time and passes through efficiently
Enter air to cavity after filter filtering, then vacuumize, add air, remove out water vapour for several times repeatedly, to device
Tool 5 is dry and is measured in cavity 1 after ozone free by ozone meter 13, takes out instrument after drying.
One preference of present embodiment is, in step S6, mixes steam solenoid valve 22 as pulse unlatching, time and arteries and veins
Jig frequency number can be set, and ozone concentration is measured by ozone meter 13 in cavity.
One preference of present embodiment is, in step S7, step S2-S6 in triplicate.
One of the present embodiment has particular application as：
Instrument is placed in low temperature plasma ozone sterilizer, closed low temperature plasma ozone sterilizer；It takes out true
Pneumatics power -30pa arrives -50pa, carries out cavity 1 and heats, in intracavitary generation plasma discharge, water vapour and ozone are added to
It mixes in steam case 21, forms mixed vapour, to intracavitary addition mixed vapour, in this way, electric discharge generates OH base by mixed vapour, with
Just it sterilizes to instrument 5, when the air pressure in cavity 1 is in 150pa-200pa, interruption electric discharge, diffusion sterilizes, vacuumizes
By the air in cavity 1 by the ozone ingredient in the decomposition cavity 1 of ozone decomposer 9.Make the return trip empty gas, and pulse vacuum is moved repeatedly
Except water vapour, it is dried in vacuo instrument 5, after having dried, restoring chamber pressure sterilizing terminates, and takes object.
In aforesaid operations, compare traditional approach, high, saturating instead of overlong time, the concentration of existing low temperature sterilizer
Power is weak, has the phenomenon that corrosion and residual, and overall structure is simply clean, and sterilizing is thorough, no dead angle.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Specific features described in conjunction with this embodiment or example, structure, material live feature and are contained at least one implementation of the invention
In example or example.In the present specification, schematic expression of the above terms may not refer to the same embodiment or example.
Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples to close
Suitable mode combines.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (6)
1. a kind of low temperature plasma ozone sterilizer, it is characterised in that：Including cavity (1), cavity (1) inner wall passes through four
Fluorine fixing piece (3,4) fixed positive electrode plate (2) and layer frame (6), the positive electrode plate (2) are electrically connected plasma power supply (14),
The cavity (1) is grounded, deployment instrument (5) on the layer frame (6)；
The cavity (1) is communicated with vacuum pump (8), and the vacuum pump (8) and cavity (1) are connected with vacuum pump solenoid valve (7)；
The cavity (1) has also been sequentially communicated mixing steam solenoid valve (22) and mixing steam case (21), the mixing steam case
(21) air inlet is connected to steam heating case (20), and the steam heating case (20) is connected to inlet valve (19) and ozone electricity
Magnet valve (18), the gas that the ozone solenoid valve (18) controls in ozone storage box (17) enter steam heating case (20), institute
It states ozone storage box (17) air inlet and is sequentially communicated ozone generator (16) and oxygen solenoid valve (15), the oxygen solenoid valve
(15) control oxygen enters in ozone generator (16)；
Pressure sensor (12) and ozone meter (13) are equipped with inside the cavity (1).
2. a kind of low temperature plasma ozone sterilizer according to claim 1, it is characterised in that：The vacuum pump (8)
Gas outlet is communicated with ozone decomposer (9).
3. a kind of low temperature plasma ozone sterilizer according to claim 1, it is characterised in that：The positive electrode plate
It (2) is mesh plate.
4. a kind of low temperature plasma ozone sterilization method of any one of -3 sterilizers, feature exist according to claim 1
In：Include the following steps：
Step S1：Instrument (5) is placed on layer frame (6), carries out being heated to 50-60 degrees Celsius after airtight cavity (1)；
Step S2：It opens oxygen solenoid valve (15), oxygen generates ozone by ozone generator (16) and enters ozone storage box
(17) it in, when ozone storage box (17) interior ozone concentration reaches setting concentration, closes oxygen solenoid valve (15)；
Step S3：It is inner that pure water by inlet valve (19) enters steam heating case (20), closes water inlet after reaching setting water level
Valve (19), the at this time inner water of heating steam heater box (20) to 60-70 degrees Celsius of generation water vapour；
Step S4：It opens vacuum pump solenoid valve (7), negative pressure is taken out in starting vacuum pump (8) into cavity (1), when cavity internal pressure passes
When sensor (12) senses that vacuum degree reaches -30pa to -50pa, vacuum pump solenoid valve (7) and vacuum pump (8) are closed；
Step S5：It opens plasma power supply (14) and generates electric discharge by positive electrode plate (2) and cavity (1)；
Step S6：Opening ozone solenoid valve (18) enters the water vapour taken away on the water surface in steam heating case (20) and enters
It mixes in steam case (21), opens mixing steam solenoid valve (22) and enter mixing steam into cavity (1), when chamber pressure reaches
Plasma power supply (14) electric discharge is interrupted when 150pa-200pa, is discharged by water vapour and ozone generation-OH base, is diffused 5-10
Minute, the mattress that goes out is carried out to instrument；
Step S7：It opens vacuum pump solenoid valve (7) starting vacuum pump (8) and takes out negative pressure into cavity, the air in cavity is passed through
Ozone decomposer (9) decomposes the ozone ingredient in cavity (1), rapid step S2-S6 is repeated, until apparatus sterilizing is qualified；
Step S8：It is vacuumized into cavity, greenhouse keeps 50-65 degree in cavity, opens intake valve (11) at this time and passes through efficient mistake
Enter air to cavity after filter filtering, then vacuumize, add air, remove out water vapour for several times repeatedly, to instrument
(5) dry and after measuring cavity (1) interior ozone free by ozone meter (13), take out it is dry after instrument.
5. a kind of low temperature plasma ozone sterilization method according to claim 4, it is characterised in that：The step S6
In, steam solenoid valve (22) are mixed as pulse unlatching, and time and pulse number can be set, and ozone concentration is measured by ozone in cavity
Meter (13) measures.
6. a kind of low temperature plasma ozone sterilization method according to claim 4, it is characterised in that：The step S7
In, step S2-S6 in triplicate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810155576.3A CN108853539A (en) | 2018-02-23 | 2018-02-23 | A kind of low temperature plasma ozone sterilizer and sterilizing methods |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810155576.3A CN108853539A (en) | 2018-02-23 | 2018-02-23 | A kind of low temperature plasma ozone sterilizer and sterilizing methods |
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| Publication Number | Publication Date |
|---|---|
| CN108853539A true CN108853539A (en) | 2018-11-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810155576.3A Pending CN108853539A (en) | 2018-02-23 | 2018-02-23 | A kind of low temperature plasma ozone sterilizer and sterilizing methods |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110464858A (en) * | 2019-07-15 | 2019-11-19 | 老肯医疗科技股份有限公司 | A kind of cabin for hydrogen peroxide low-temperature plasma sterilizer |
| CN110693692A (en) * | 2019-10-09 | 2020-01-17 | 华中科技大学 | Plasma activated mist preparation device |
| CN115161166A (en) * | 2022-07-27 | 2022-10-11 | 安徽大学 | Experimental device for low temperature plasma inactivation microorganism |
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| CN103083698A (en) * | 2013-02-16 | 2013-05-08 | 淄博康元医疗器械有限公司 | Ternary complex sterilizing method and device |
| CN105999332A (en) * | 2016-07-07 | 2016-10-12 | 连云港佑源医药设备制造有限公司 | Low-temperature plasma formaldehyde sterilizer and formaldehyde removing method |
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| US5184046A (en) * | 1990-09-28 | 1993-02-02 | Abtox, Inc. | Circular waveguide plasma microwave sterilizer apparatus |
| JPH11505166A (en) * | 1995-06-07 | 1999-05-18 | ジェイコブ，アディル | Dry sterilization method and apparatus for medical equipment |
| CN1153668A (en) * | 1995-10-27 | 1997-07-09 | 庄臣及庄臣医药有限公司 | Vapor sterilization using inorganic hydrogen peroxide complexes |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110464858A (en) * | 2019-07-15 | 2019-11-19 | 老肯医疗科技股份有限公司 | A kind of cabin for hydrogen peroxide low-temperature plasma sterilizer |
| CN110693692A (en) * | 2019-10-09 | 2020-01-17 | 华中科技大学 | Plasma activated mist preparation device |
| CN115161166A (en) * | 2022-07-27 | 2022-10-11 | 安徽大学 | Experimental device for low temperature plasma inactivation microorganism |
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